Rroxscaffold_5G00332880

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004649
Physical Location & Seq
Forward (+)
233745 .. 252890
19146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332880.1

Sequence Viewer

Length: 3747 bp
ATGGCGGACTCGGCAGGGACGACCCTGATGGATCTGATAACGGCGGACCCTTCCACAGTATCAGCTTCCACCTCCTCAACGACGTCGTCTTCCTCTTCCGCGCCTCCTCCTCCTCCTCCTTATGCGGCTTCACCCCCACCGCCCAGAGGCACCAGCCCCGGCTCCGCTCTCGGAAAGCCTGCTGTCGAGAAGAGGTCCAAGAGGGCTGCCCTTATGCAGATCCAGAACGACACCATCTCTGCCGCCAAAGCTGCTCTCAATCCCGTTCGCACCAACATCATCATGCCCCAGAAGCACAGGCACAAGCAGAAAAAGCCTGTTTCATATGCTCAACTTGCAAGGAGCATTCACGAACTAGCTGCCGCCTCCGATCAGAAAAGTTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTCGCCGTCTACAATTCTGTTGACCCCTCTGTCGCCCCTTCTCTTCTCATGCTGAATCAGCAGTGTGAGGATAAGAGCGTTCTGCGATATGTCTATTATTACTTGGCCAGGATTTTATCAGATACTGGTGCACAAGGTGTAACCACAGGCGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTGTTGGAGGGGTAACTAGAGCTGATGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAGGCTTCAAATGCTGATGCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCTCCATCAACAAATTCTGAAATTTTGTCCAAATTGTATGAAATTGTGTTTGGCATTCTTGATAAGGTTGCTGATGGCCCGCAAAAACGTAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAGGAGTTTATCATCCGAAGTAACTTGCAATATGCTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTGTCATTTGCTGATCCTGTTGCTGTAAGGCATGCTTTGGAAATTCTGTCTGAGCTAGCCACAAAAGACCCTTATGCAGTTGCGATGGGCTTAGGGAAACATGCAGAGCCTGGAGGGGCTTTGCAGGATGTTCTTCATTTGCATGATGTTCTTGCTAGAGTTGCTCTAGCTAGGTTGTGTTATACAATATCAAGAGCACGAGTATTGGATGACAGGGCTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTCTGTTTTGAGGCAATCTTATGTATATTGGGAAAACACGATAACACAGAAAGGACTGATGATCGAGCTGCCGGATGGTATCGATTAACTAGAGAGATTCTCAAGTTGCCAGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGTTCAAAAGACAAGGCGTCCACAACCCCTTATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCAGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTCGGGCTGCCGCATTTGCTCTAGGCATACAGGATATCGACGAATCAGTGCATGTTAACACATTTTCTGAGGCTCCTGATTCACGCGAGATAGATAGTAGTGAAGCCTCACATCCTGAGAGTATCCGAAGAACCTCTTCCTTACCTACTGGGGTAGGTGGCAAGGATACAATAGCTAGTTTGTTAGCTTCATTAATGGAAGTTGTGCGGACAACTGTGGCATGCGAGTGTGTTTATGTTCGAGCAATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCACATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTATCTGATCCAGCTTGGCCAGCCACGCTCTTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCTGTCACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCCCCGGGGAAGATCGATGCTGATGTACTACAACTACTGTGGAAGACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGGCTTACATCAGTTGACAGGGTATCTGCATCTGATCCAAAGGCAGCCCTGGCTTTGCAGAGATTAGTACAAGCAGCAGTATGGTTTCTTGGAGAAAATGCAAACTATGCTGCTTCTGAATATGCTTGGGAATCCGCAACCCCTCCTGGTACTGCATTGATGATGCTAGATGCAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCGGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCACAGGGCGGAGTGCAGTCTCAGTTTTCTGACATGCATCCCAGTAATGGAGAAGACCAAGGAGCTAGTGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACGGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCGAAACTTATCGACATCTATCGCACCAGGCACAATATTAGTGCATCAACTGGTTTGAGTGATCCAGCTGTTGCTACTGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCTGTAGAGCCTGATGTGCTTGATGATGACCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGACGGACTGTTAGGAAATAATGCACCAGCAATGAGCAGGGTTAATGAATTTCTAGCTGGTGCTGGAACTGATGCTCCTGATGTTGATGAGGAAAACATCATCTCTAGGCCGTCAGTTGGTTACGATGATATGTGGGCAAAGACCCTTTTAGAGACTTCAGAACTGGAGGAAGAAGACGCGAGGTCATCCGGGACATCTTCACCTGAATCAACAGGCTCGGTCGAAACTTCCATATCATCGCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGATATAGCAATCCATCTATGGGGGGTCCCTCATTCAATGAGGGTTTAGGATCTCCAATTAGAGAAGAGCCTCCGCCATACTCATCACCTGCAACGCAAAGATTTGAGTCGTTTGAGAATCCCTTAGCTGGGCATGGATCCCAGAGTTTTGGATCCCAAGATGACGAACGCATTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACAGCCGGAGAGGAGGTTGATATCGAGTACGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCTGGGAGGGATGGGAAAATGGCTGGTCTGGTCCCTGTCCTCTATGTTATGGGCTTGCTTGCGGTACCTGGAAGTTTGAGTCTCCAGTCTGGTGCCACATTAGTGAATCTCCTGGTACATCCACATTTTTTGAGCAGTGGGAGTTCCGTGAGAATCAGCCAGGATGCTGGTCATTGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1248

Amino Acids

134.67

Weight (kDa)

5.55

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1151 - 1192 7.5e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3349
AasI GACNNNNNNGTC 1 cut(s) 446
AatII GACGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 2633
Acc36I ACCTGC 1 cut(s) 3349
Acc65I GGTACC 1 cut(s) 3628
AccB1I GGYRCC 3 cut(s) 149, 3628, 3656
AccB7I CCANNNNNTGG 1 cut(s) 2102
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 426
AccII CGCG 3 cut(s) 101, 1608, 3128
AcoI YGGCCR 3 cut(s) 522, 1862, 3230
AcsI RAATTY 8 cut(s) 695, 751, 759, 1011, 2459, 2853, 2996, 3493
AcuI CTGAAG 1 cut(s) 3090
AcyI GRCGYC 2 cut(s) 83, 1411
AdeI CACNNNGTG 1 cut(s) 554
AflII CTTAAG 1 cut(s) 2675
AgsI TTSAA 8 cut(s) 681, 724, 965, 1207, 1400, 1879, 1946, 3289
AhdI GACNNNNNGTC 2 cut(s) 1410, 3130
AjiI CACGTC 1 cut(s) 406
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AleI CACNNNNGTG 1 cut(s) 3665
AloI GAACNNNNNNTCC 2 cut(s) 3007, 3039
Alw21I GWGCWC 3 cut(s) 550, 910, 1168
Alw26I GTCTC 3 cut(s) 2499, 3095, 3650
Alw44I GTGCAC 1 cut(s) 546
AlwNI CAGNNNCTG 3 cut(s) 542, 1079, 2888
Ama87I CYCGRG 1 cut(s) 1975
AoxI GGCC 8 cut(s) 522, 814, 1475, 1862, 2551, 2762, 3056, 3230
ApaLI GTGCAC 1 cut(s) 546
ApoI RAATTY 8 cut(s) 695, 751, 759, 1011, 2459, 2853, 2996, 3493
AseI ATTAAT 1 cut(s) 1715
Asp700I GAANNNNTTC 2 cut(s) 1468, 1657
Asp718I GGTACC 1 cut(s) 3628
AspLEI GCGC 2 cut(s) 103, 2634
AspS9I GGNCC 7 cut(s) 46, 195, 815, 2036, 2762, 3278, 3595
AsuC2I CCSGG 4 cut(s) 159, 1976, 1977, 3139
AsuHPI GGTGA 6 cut(s) 123, 409, 2444, 2957, 3141, 3330
AsuNHI GCTAGC 1 cut(s) 1024
AvaI CYCGRG 1 cut(s) 1975
AvaII GGWCC 5 cut(s) 46, 195, 2036, 3278, 3595
BaeGI GKGCMC 2 cut(s) 550, 2608
BalI TGGCCA 2 cut(s) 524, 1864
BamHI GGATCC 5 cut(s) 573, 928, 1234, 3389, 3404
BanI GGYRCC 3 cut(s) 149, 3628, 3656
BauI CACGAG 1 cut(s) 1167
BbsI GAAGAC 6 cut(s) 81, 715, 922, 2021, 2535, 3129
Bbv12I GWGCWC 3 cut(s) 550, 910, 1168
BceAI ACGGC 4 cut(s) 57, 407, 3043, 3245
BcgI CGANNNNNNTGC 4 cut(s) 341, 375, 2536, 2570
BciVI GTATCC 2 cut(s) 1655, 1681
BclI TGATCA 2 cut(s) 1816, 2619
BcnI CCSGG 4 cut(s) 159, 1976, 1977, 3139
BcoDI GTCTC 3 cut(s) 2499, 3095, 3650
BfmI CTRYAG 4 cut(s) 669, 2351, 2472, 2889
BfrI CTTAAG 1 cut(s) 2675
BfuAI ACCTGC 1 cut(s) 3349
BfuI GTATCC 2 cut(s) 1655, 1681
BglI GCCNNNNNGGC 2 cut(s) 2443, 2761
BglII AGATCT 2 cut(s) 1196, 2426
BmcAI AGTACT 1 cut(s) 1482
Bme18I GGWCC 5 cut(s) 46, 195, 2036, 3278, 3595
BmeRI GACNNNNNGTC 2 cut(s) 1410, 3130
BmeT110I CYCGRG 1 cut(s) 1975
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 7 cut(s) 46, 195, 815, 2036, 2762, 3278, 3595
BmrI ACTGGG 3 cut(s) 598, 1680, 2511
BmtI GCTAGC 1 cut(s) 1028
BmuI ACTGGG 3 cut(s) 598, 1680, 2511
BpiI GAAGAC 6 cut(s) 81, 715, 922, 2021, 2535, 3129
BplI GAGNNNNNCTC 2 cut(s) 1332, 1364
BpmI CTGGAG 5 cut(s) 1101, 1905, 3134, 3264, 3632
Bpu10I CCTNAGC 2 cut(s) 1060, 3376
BpuEI CTTGAG 2 cut(s) 1334, 2385
BpuMI CCSGG 4 cut(s) 159, 1976, 1977, 3139
Bsa29I ATCGAT 2 cut(s) 1330, 1986
BsaHI GRCGYC 2 cut(s) 83, 1411
BsaXI ACNNNNNCTCC 6 cut(s) 724, 754, 3007, 3037, 3498, 3528
Bse3DI GCAATG 3 cut(s) 1773, 2391, 2985
BseCI ATCGAT 2 cut(s) 1330, 1986
BseMI GCAATG 3 cut(s) 1773, 2391, 2985
BseMII CTCAG 5 cut(s) 1011, 1214, 1581, 1629, 2510
BseRI GAGGAG 7 cut(s) 64, 96, 99, 102, 105, 1217, 3524
BseSI GKGCMC 2 cut(s) 550, 2608
BseX3I CGGCCG 1 cut(s) 3230
BseYI CCCAGC 4 cut(s) 940, 1238, 2376, 3380
BsgI GTGCAG 2 cut(s) 1526, 2510
Bsh1236I CGCG 3 cut(s) 101, 1608, 3128
Bsh1285I CGRYCG 2 cut(s) 3171, 3233
BshFI GGCC 8 cut(s) 524, 816, 1477, 1864, 2553, 2764, 3058, 3232
BshNI GGYRCC 3 cut(s) 149, 3628, 3656
BshVI ATCGAT 2 cut(s) 1330, 1986
BsiEI CGRYCG 2 cut(s) 3171, 3233
BsiHKAI GWGCWC 3 cut(s) 550, 910, 1168
BsiHKCI CYCGRG 1 cut(s) 1975
BsiSI CCGG 6 cut(s) 159, 1320, 1976, 3138, 3233, 3504
BslFI GGGAC 6 cut(s) 31, 859, 2556, 3154, 3264, 3581
BsmAI GTCTC 3 cut(s) 2499, 3095, 3650
BsmFI GGGAC 6 cut(s) 31, 859, 2556, 3154, 3264, 3581
BsmI GAATGC 3 cut(s) 345, 792, 2363
BsnI GGCC 8 cut(s) 524, 816, 1477, 1864, 2553, 2764, 3058, 3232
BsoBI CYCGRG 1 cut(s) 1975
Bsp1286I GDGCHC 4 cut(s) 550, 910, 1168, 2608
BspANI GGCC 8 cut(s) 524, 816, 1477, 1864, 2553, 2764, 3058, 3232
BspCNI CTCAG 5 cut(s) 1012, 1213, 1582, 1630, 2509
BspDI ATCGAT 2 cut(s) 1330, 1986
BspFNI CGCG 3 cut(s) 101, 1608, 3128
BspMI ACCTGC 1 cut(s) 3349
BspOI GCTAGC 1 cut(s) 1028
BspQI GCTCTTC 2 cut(s) 2664, 3312
BspT107I GGYRCC 3 cut(s) 149, 3628, 3656
BspTI CTTAAG 1 cut(s) 2675
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 3 cut(s) 1773, 2391, 2985
BssNI GRCGYC 2 cut(s) 83, 1411
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 2533, 2941
Bst2BI CACGAG 1 cut(s) 1167
Bst4CI ACNGT 5 cut(s) 58, 956, 1738, 2010, 2958
Bst6I CTCTTC 7 cut(s) 100, 185, 465, 1663, 1944, 2664, 3312
BstACI GRCGYC 2 cut(s) 83, 1411
BstAFI CTTAAG 1 cut(s) 2675
BstAPI GCANNNNNTGC 1 cut(s) 2222
BstDEI CTNAG 9 cut(s) 902, 911, 1020, 1060, 1200, 1590, 1638, 2496, 3376
BstEII GGTNACC 1 cut(s) 2068
BstFNI CGCG 3 cut(s) 101, 1608, 3128
BstHHI GCGC 2 cut(s) 103, 2634
BstMAI GTCTC 3 cut(s) 2499, 3095, 3650
BstMCI CGRYCG 2 cut(s) 3171, 3233
BstNSI RCATGY 6 cut(s) 1004, 1073, 1577, 1746, 1904, 2512
BstPI GGTNACC 1 cut(s) 2068
BstSFI CTRYAG 4 cut(s) 669, 2351, 2472, 2889
BstSLI GKGCMC 2 cut(s) 550, 2608
BstUI CGCG 3 cut(s) 101, 1608, 3128
BstV2I GAAGAC 6 cut(s) 81, 715, 922, 2021, 2535, 3129
BstXI CCANNNNNNTGG 2 cut(s) 3401, 3731
BstZI CGGCCG 1 cut(s) 3230
Bsu15I ATCGAT 2 cut(s) 1330, 1986
BsuI GTATCC 2 cut(s) 1655, 1681
BsuRI GGCC 8 cut(s) 524, 816, 1477, 1864, 2553, 2764, 3058, 3232
BsuTUI ATCGAT 2 cut(s) 1330, 1986
BtgZI GCGATG 2 cut(s) 1067, 3171
BtrI CACGTC 1 cut(s) 406
BtsI GCAGTG 4 cut(s) 485, 2052, 3468, 3706
BtsIMutI CAGTG 6 cut(s) 485, 1575, 2052, 2374, 3468, 3706
BveI ACCTGC 1 cut(s) 3349
CaiI CAGNNNCTG 3 cut(s) 542, 1079, 2888
CfoI GCGC 2 cut(s) 103, 2634
Cfr13I GGNCC 7 cut(s) 46, 195, 815, 2036, 2762, 3278, 3595
Cfr9I CCCGGG 1 cut(s) 1975
ClaI ATCGAT 2 cut(s) 1330, 1986
CseI GACGC 2 cut(s) 1400, 3134
CspCI CAANNNNNGTGG 4 cut(s) 1991, 2026, 3675, 3710
DdeI CTNAG 9 cut(s) 902, 911, 1020, 1060, 1200, 1590, 1638, 2496, 3376
DraIII CACNNNGTG 1 cut(s) 554
DrdI GACNNNNNNGTC 1 cut(s) 446
DriI GACNNNNNGTC 2 cut(s) 1410, 3130
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 3 cut(s) 522, 1862, 3230
EagI CGGCCG 1 cut(s) 3230
Eam1104I CTCTTC 7 cut(s) 100, 185, 465, 1663, 1944, 2664, 3312
Eam1105I GACNNNNNGTC 2 cut(s) 1410, 3130
EarI CTCTTC 7 cut(s) 100, 185, 465, 1663, 1944, 2664, 3312
EciI GGCGGA 4 cut(s) 20, 59, 2499, 3315
EclXI CGGCCG 1 cut(s) 3230
Eco130I CCWWGG 2 cut(s) 2533, 2941
Eco147I AGGCCT 1 cut(s) 2553
Eco32I GATATC 2 cut(s) 1558, 3520
Eco47I GGWCC 5 cut(s) 46, 195, 2036, 3278, 3595
Eco52I CGGCCG 1 cut(s) 3230
Eco57I CTGAAG 1 cut(s) 3090
Eco88I CYCGRG 1 cut(s) 1975
Eco91I GGTNACC 1 cut(s) 2068
EcoO109I RGGNCCY 1 cut(s) 3278
EcoO65I GGTNACC 1 cut(s) 2068
EcoRV GATATC 2 cut(s) 1558, 3520
EcoT14I CCWWGG 2 cut(s) 2533, 2941
EcoT22I ATGCAT 1 cut(s) 2514
ErhI CCWWGG 2 cut(s) 2533, 2941
FalI AAGNNNNNCTT 4 cut(s) 988, 1020, 1642, 1674
FaqI GGGAC 6 cut(s) 31, 859, 2556, 3154, 3264, 3581
FauI CCCGC 2 cut(s) 825, 2324
FauNDI CATATG 2 cut(s) 325, 736
FbaI TGATCA 2 cut(s) 1816, 2619
FblI GTMKAC 1 cut(s) 426
FspI TGCGCA 1 cut(s) 2633
GlaI GCGC 2 cut(s) 102, 2633
GsaI CCCAGC 4 cut(s) 944, 1242, 2380, 3384
GsuI CTGGAG 5 cut(s) 1101, 1905, 3134, 3264, 3632
HaeIII GGCC 8 cut(s) 524, 816, 1477, 1864, 2553, 2764, 3058, 3232
HapII CCGG 6 cut(s) 159, 1320, 1976, 3138, 3233, 3504
HgaI GACGC 2 cut(s) 1400, 3134
HhaI GCGC 2 cut(s) 103, 2634
Hin1I GRCGYC 2 cut(s) 83, 1411
Hin6I GCGC 2 cut(s) 101, 2632
HinP1I GCGC 2 cut(s) 101, 2632
HincII GTYRAC 3 cut(s) 439, 1579, 2131
HindII GTYRAC 3 cut(s) 439, 1579, 2131
HindIII AAGCTT 1 cut(s) 717
HpaI GTTAAC 1 cut(s) 1579
HpaII CCGG 6 cut(s) 159, 1320, 1976, 3138, 3233, 3504
HphI GGTGA 6 cut(s) 123, 409, 2444, 2957, 3141, 3330
Hpy166II GTNNAC 8 cut(s) 427, 439, 548, 1415, 1579, 1802, 2131, 3227
Hpy8I GTNNAC 8 cut(s) 427, 439, 548, 1415, 1579, 1802, 2131, 3227
Hpy99I CGWCG 3 cut(s) 85, 88, 1565
HpyAV CCTTC 6 cut(s) 60, 465, 718, 1369, 1377, 3222
HpyCH4III ACNGT 5 cut(s) 58, 956, 1738, 2010, 2958
HpyCH4IV ACGT 3 cut(s) 83, 405, 826
HpyF3I CTNAG 9 cut(s) 902, 911, 1020, 1060, 1200, 1590, 1638, 2496, 3376
HpySE526I ACGT 3 cut(s) 83, 405, 826
Hsp92I GRCGYC 2 cut(s) 83, 1411
HspAI GCGC 2 cut(s) 101, 2632
KflI GGGWCCC 1 cut(s) 3278
KpnI GGTACC 1 cut(s) 3632
Ksp22I TGATCA 2 cut(s) 1816, 2619
KspAI GTTAAC 1 cut(s) 1579
LguI GCTCTTC 2 cut(s) 2664, 3312
LmnI GCTCC 7 cut(s) 167, 342, 745, 1600, 2030, 2537, 3028
MaeII ACGT 3 cut(s) 83, 405, 826
MaeIII GTNAC 6 cut(s) 556, 625, 881, 1933, 2068, 3068
MbiI CCGCTC 1 cut(s) 167
MhlI GDGCHC 4 cut(s) 550, 910, 1168, 2608
MlsI TGGCCA 2 cut(s) 524, 1864
MluNI TGGCCA 2 cut(s) 524, 1864
MlyI GAGTC 5 cut(s) 2, 1257, 1531, 3368, 3652
MmeI TCCRAC 2 cut(s) 598, 2008
Mox20I TGGCCA 2 cut(s) 524, 1864
Mph1103I ATGCAT 1 cut(s) 2514
MroXI GAANNNNTTC 2 cut(s) 1468, 1657
MscI TGGCCA 2 cut(s) 524, 1864
MslI CAYNNNNRTG 6 cut(s) 281, 1110, 1747, 2414, 2643, 3665
Msp20I TGGCCA 2 cut(s) 524, 1864
MspA1I CMGCKG 3 cut(s) 2376, 2840, 2888
MspCI CTTAAG 1 cut(s) 2675
MspI CCGG 6 cut(s) 159, 1320, 1976, 3138, 3233, 3504
Mva1269I GAATGC 3 cut(s) 345, 792, 2363
MvnI CGCG 3 cut(s) 101, 1608, 3128
NciI CCSGG 4 cut(s) 159, 1976, 1977, 3139
NdeI CATATG 2 cut(s) 325, 736
NheI GCTAGC 1 cut(s) 1024
NmuCI GTSAC 1 cut(s) 1933
NsbI TGCGCA 1 cut(s) 2633
NsiI ATGCAT 1 cut(s) 2514
NspI RCATGY 6 cut(s) 1004, 1073, 1577, 1746, 1904, 2512
OliI CACNNNNGTG 1 cut(s) 3665
PaeI GCATGC 3 cut(s) 1004, 1746, 1904
PaqCI CACCTGC 1 cut(s) 3349
PasI CCCWGGG 1 cut(s) 3566
PceI AGGCCT 1 cut(s) 2553
PciSI GCTCTTC 2 cut(s) 2664, 3312
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 3 cut(s) 345, 792, 2363
PdmI GAANNNNTTC 2 cut(s) 1468, 1657
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 2102
PfoI TCCNGGA 3 cut(s) 931, 1230, 3137
PleI GAGTC 5 cut(s) 2, 1256, 1530, 3367, 3651
PpsI GAGTC 5 cut(s) 2, 1256, 1530, 3367, 3651
PpuMI RGGWCCY 1 cut(s) 3278
PshBI ATTAAT 1 cut(s) 1715
Psp5II RGGWCCY 1 cut(s) 3278
PspEI GGTNACC 1 cut(s) 2068
PspFI CCCAGC 4 cut(s) 940, 1238, 2376, 3380
PspPI GGNCC 7 cut(s) 46, 195, 815, 2036, 2762, 3278, 3595
PspPPI RGGWCCY 1 cut(s) 3278
PstNI CAGNNNCTG 3 cut(s) 542, 1079, 2888
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 3 cut(s) 2376, 2840, 2888
RseI CAYNNNNRTG 6 cut(s) 281, 1110, 1747, 2414, 2643, 3665
SapI GCTCTTC 2 cut(s) 2664, 3312
Sau96I GGNCC 7 cut(s) 46, 195, 815, 2036, 2762, 3278, 3595
ScaI AGTACT 1 cut(s) 1482
SchI GAGTC 5 cut(s) 2, 1257, 1531, 3368, 3652
SduI GDGCHC 4 cut(s) 550, 910, 1168, 2608
SfcI CTRYAG 4 cut(s) 669, 2351, 2472, 2889
SinI GGWCC 5 cut(s) 46, 195, 2036, 3278, 3595
SmaI CCCGGG 1 cut(s) 1977
SmiMI CAYNNNNRTG 6 cut(s) 281, 1110, 1747, 2414, 2643, 3665
SmlI CTYRAG 3 cut(s) 1349, 2400, 2675
SmoI CTYRAG 3 cut(s) 1349, 2400, 2675
SphI GCATGC 3 cut(s) 1004, 1746, 1904
SseBI AGGCCT 1 cut(s) 2553
SspI AATATT 1 cut(s) 2809
StuI AGGCCT 1 cut(s) 2553
StyI CCWWGG 2 cut(s) 2533, 2941
TaaI ACNGT 5 cut(s) 58, 956, 1738, 2010, 2958
TaiI ACGT 3 cut(s) 86, 408, 829
TaqII GACCGA 1 cut(s) 3157
TatI WGTACW 4 cut(s) 1213, 1480, 1996, 2182
TauI GCSGC 5 cut(s) 128, 245, 365, 1535, 2399
TscAI CASTG 6 cut(s) 485, 1575, 2059, 2374, 3475, 3706
TseFI GTSAC 1 cut(s) 1933
Tsp45I GTSAC 1 cut(s) 1933
TspGWI ACGGA 3 cut(s) 2678, 2967, 3700
TspMI CCCGGG 1 cut(s) 1975
TspRI CASTG 6 cut(s) 485, 1575, 2059, 2374, 3475, 3706
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 2102
Vha464I CTTAAG 1 cut(s) 2675
VneI GTGCAC 1 cut(s) 546
VpaK11BI GGWCC 5 cut(s) 46, 195, 2036, 3278, 3595
VspI ATTAAT 1 cut(s) 1715
XapI RAATTY 8 cut(s) 695, 751, 759, 1011, 2459, 2853, 2996, 3493
XceI RCATGY 6 cut(s) 1004, 1073, 1577, 1746, 1904, 2512
XcmI CCANNNNNNNNNTGG 2 cut(s) 585, 2540
XmaI CCCGGG 1 cut(s) 1975
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 1468, 1657
ZraI GACGTC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 1482
Zsp2I ATGCAT 1 cut(s) 2514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.