Rw4G000720

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004649
Physical Location & Seq
Reverse (-)
1423748 .. 1443180
19433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000720.1

Sequence Viewer

Length: 3648 bp
ATGGCGGACTCGTCAGGGACGACCCTGATGGATCTGATAACGGCGGACCCTTCCACAGTATCAGCTTCCACCTCCTCAACGACGTCGTCTTCGTCTTCGTCTTCCGCGCCTCCTCCTCCTCCTCCTTATGCGGCTTCACCCCCACCGCCCAGAGGCACCAGCCCCGGCTCCGCTCTCGGAAAGCCTGCTGTCGAGAAGAGGTCCAAGAGGGCTGCGCTTATGCAGATCCAGAACGACACCATCTCTGCCGCCAAAGCTGCTCTCAATCCCGTTCGCACCAACATCATCATGCCCCAGAAGCACAGGCACAAGCAGAAAAAGCCTGTTTCATATGCTCAACTTGCAAGGAGCATTCACGAACTAGCTGCCGCCTCCGATCAGAAAAGTTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTCGCCGTCTACAATTCTGTTGACCCCTCTGTCGCCCCTTCTCTTCTCATGCTGAATCAGCAGTGTGAGGATAAGAGCGTTCTGCGATATGTCTATTATTACTTGGCCAGGATTTTATCAGATACTGGTGCACAAGGTGTAACCACAGGCGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTATTGGAGGGGTAACTAGAGCTGACGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAAGCTTCAAATGCTGATGCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCTCCATCAACAAATTCTGAAATTTTGTCCAAATTGTATGAAATTGTGTTTGGCATTCTTGAGAAGGTTGCTGATGGCCCGCAAAAACGCAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAAGAGTTTATCATCCGAAGTAACTTGCAATATGCTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTGTCATTTGCTGATCCTGTTGCTGTAAGGCATGCTTTGGAAATTCTGTCTGAGCTAGCCACAAAAGACCCGTATGCAGTTGCAATGGGCTTAGGGAAACATGCAGAGCCTGGAGGGGCTTTGCAGGATGTTCTCCATTTGCATGATGTTCTTGCTAGAGTTGCTCTAGCTAGGTTGTGTTATACAATATCAAGAGCACGAGCATTGGATGACAGGCCTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTCTGTTTTGAGGCAATCTTATGTATATTGGGAAAACACGATAACACAGAAAGGACTGATGATCGAGCTGCTGGATGGTATCGATTAACTAGAGAGATTCTCAAGTTGCCAGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGTTCAAAAGACAAGGCGTCCACAACCCCTTATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCAGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTCGGGCTGCCGCATTTGCTCTAGGCATACAGGATATCGACGAATCAGTGCATGTTAACACATTTTCTGAGACTCCTGATTCACGCGAGATAGATAGTAGTGAAGCCTCACATCCTGAGAGTATCCGAAGAACCTCTTCCTTACCTACTGGGGTAGGTGGCAAGGATACAATAGCTAGTTTGTTAGCTTCATTAATGGAAGTTGTGCGGACAACTGTGGCATGCGAGTGTGTTTATGTTCGAGCAATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCACATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTTTCTGATCCGGCTTGGCCAGCCACGCTCTTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCCGTCACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCCCCGGGGAAGATCGATGCTGATGTACTACAACTACTGTGGAAGACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGGCTTACATCAGTTGACAGGGTATCTGCATCTGATCCAAAGGCAGCCCTGGCTTTACAGAGATTAGTACAAGCAGCAGTAAGCATCTTAATGACCAGTTGGTTTCTTGGAGAAAATGCAAACTATGCTGCTTCTGAATATGCTTGGGAATCCGCAACCCCTCCTGGTACCGCATTGATGATGCTAGATGCAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCACAGGGCGGAGTGCAGTCTCAGTTTTCTGACATGCATCCCAGTAATGGAGAAGACCAAGGAGCTAGTGGCACAGGCCTTGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTTTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCGAAACTTATCGACATCTATCGCACCAGGCATAATATTAGTGCATCAACTGGTTTGAGTGATCCAGCTGTTGCTACTGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCTGTAGAGCCTGATGTGCTTGATGATGACCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGACGGACTGTTAGGAAATAATGCACCAGCAATGAGCAGGGTTAATGAATTTCTAGCTGGTGCTGGAACTGATGCTCCTGATGTTGATGAGGAAAACATCATCTCTAGGCCGTCAGTTGGTTACGATGATATGTGGGCAAAGACCCTTTTAGAGACTTCAGAACTGGAGGAAGAAGACGCGAGGTCATCCGGGACATCTTCACCTGAATCAACAGGCTCGGTCGAAACTTCCATATCATCGCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGATATAGCAATCCATCTATGGGGGGTCCCTCATTCAATGAGGGTTTAGGATCTCCAATTAGAGAAGAGCCTCCGCCATACTCATCACCTGCAATGCAAAGATTTGAGTCGTTTGAGAATCCCTTAGCTGGGCATGGATCCCAGAGTTTTGGATCCCAAGATGACGAACGCAGTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACAGCTGGAGAGGAGGTTGATATCGAGTACGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCCGGGAGGGATGGGAAAATGGCTGGTCTGGTCCCTGTCCTCTATGTTAGTCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1215

Amino Acids

131.43

Weight (kDa)

5.48

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1159 - 1200 7.2e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3373
AasI GACNNNNNNGTC 1 cut(s) 452
AatII GACGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 2657
Acc36I ACCTGC 1 cut(s) 3373
Acc65I GGTACC 1 cut(s) 2288
AccB1I GGYRCC 2 cut(s) 155, 2288
AccB7I CCANNNNNTGG 1 cut(s) 2108
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 432
AccII CGCG 3 cut(s) 107, 1614, 3152
AcoI YGGCCR 4 cut(s) 528, 1868, 1935, 3254
AcsI RAATTY 8 cut(s) 701, 757, 765, 1017, 2483, 2877, 3020, 3517
AcuI CTGAAG 1 cut(s) 3114
AcyI GRCGYC 2 cut(s) 83, 1417
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 8 cut(s) 651, 959, 1221, 1488, 2004, 2190, 2290, 3551
AflII CTTAAG 1 cut(s) 2699
AgsI TTSAA 8 cut(s) 687, 730, 971, 1213, 1406, 1885, 1952, 3313
AhdI GACNNNNNGTC 2 cut(s) 1416, 3154
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 8 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2284, 2822
AjuI GAANNNNNNNTTGG 2 cut(s) 777, 809
AloI GAACNNNNNNTCC 2 cut(s) 3031, 3063
Alw21I GWGCWC 3 cut(s) 556, 916, 1174
Alw26I GTCTC 3 cut(s) 1592, 2523, 3119
Alw44I GTGCAC 1 cut(s) 552
AlwNI CAGNNNCTG 3 cut(s) 548, 1085, 2912
Ama87I CYCGRG 2 cut(s) 1981, 3590
ApaLI GTGCAC 1 cut(s) 552
ApoI RAATTY 8 cut(s) 701, 757, 765, 1017, 2483, 2877, 3020, 3517
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AseI ATTAAT 1 cut(s) 1721
Asp700I GAANNNNTTC 2 cut(s) 1474, 1663
Asp718I GGTACC 1 cut(s) 2288
AspLEI GCGC 3 cut(s) 109, 217, 2658
AspS9I GGNCC 7 cut(s) 46, 201, 821, 2042, 2786, 3302, 3619
AsuC2I CCSGG 6 cut(s) 165, 1982, 1983, 3163, 3591, 3592
AsuHPI GGTGA 6 cut(s) 129, 415, 2468, 2981, 3165, 3354
AsuNHI GCTAGC 1 cut(s) 1030
AvaI CYCGRG 2 cut(s) 1981, 3590
AvaII GGWCC 5 cut(s) 46, 201, 2042, 3302, 3619
BaeGI GKGCMC 2 cut(s) 556, 2632
BalI TGGCCA 2 cut(s) 530, 1870
BamHI GGATCC 5 cut(s) 579, 934, 1240, 3413, 3428
BanI GGYRCC 2 cut(s) 155, 2288
BauI CACGAG 1 cut(s) 1173
BbsI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2559, 3153
Bbv12I GWGCWC 3 cut(s) 556, 916, 1174
BceAI ACGGC 5 cut(s) 57, 413, 1922, 3067, 3269
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 8 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2286, 2824
BciVI GTATCC 2 cut(s) 1661, 1687
BclI TGATCA 2 cut(s) 1822, 2643
BcnI CCSGG 6 cut(s) 165, 1982, 1983, 3163, 3591, 3592
BcoDI GTCTC 3 cut(s) 1592, 2523, 3119
BfmI CTRYAG 4 cut(s) 675, 2375, 2496, 2913
BfrI CTTAAG 1 cut(s) 2699
BfuAI ACCTGC 1 cut(s) 3373
BfuI GTATCC 2 cut(s) 1661, 1687
BglI GCCNNNNNGGC 2 cut(s) 2467, 2785
BglII AGATCT 2 cut(s) 1202, 2450
BmcAI AGTACT 1 cut(s) 1488
Bme18I GGWCC 5 cut(s) 46, 201, 2042, 3302, 3619
BmeRI GACNNNNNGTC 2 cut(s) 1416, 3154
BmeT110I CYCGRG 2 cut(s) 1981, 3590
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 7 cut(s) 46, 201, 821, 2042, 2786, 3302, 3619
BmrI ACTGGG 3 cut(s) 604, 1686, 2535
BmtI GCTAGC 1 cut(s) 1034
BmuI ACTGGG 3 cut(s) 604, 1686, 2535
BpiI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2559, 3153
BplI GAGNNNNNCTC 2 cut(s) 1338, 1370
BpmI CTGGAG 5 cut(s) 1107, 1911, 3158, 3288, 3549
Bpu10I CCTNAGC 2 cut(s) 1066, 3400
BpuEI CTTGAG 3 cut(s) 824, 1340, 2409
BpuMI CCSGG 6 cut(s) 165, 1982, 1983, 3163, 3591, 3592
Bsa29I ATCGAT 2 cut(s) 1336, 1992
BsaHI GRCGYC 2 cut(s) 83, 1417
BsaXI ACNNNNNCTCC 6 cut(s) 730, 760, 3031, 3061, 3522, 3552
Bse3DI GCAATG 5 cut(s) 1065, 1779, 2415, 3009, 3375
BseBI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2286, 2824
BseCI ATCGAT 2 cut(s) 1336, 1992
BseMI GCAATG 5 cut(s) 1065, 1779, 2415, 3009, 3375
BseMII CTCAG 5 cut(s) 1017, 1220, 1587, 1635, 2534
BseRI GAGGAG 7 cut(s) 64, 102, 105, 108, 111, 1223, 3548
BseSI GKGCMC 2 cut(s) 556, 2632
BseX3I CGGCCG 1 cut(s) 3254
BseYI CCCAGC 4 cut(s) 946, 1244, 2400, 3404
BsgI GTGCAG 2 cut(s) 1532, 2534
Bsh1236I CGCG 3 cut(s) 107, 1614, 3152
Bsh1285I CGRYCG 2 cut(s) 3195, 3257
BshNI GGYRCC 2 cut(s) 155, 2288
BshVI ATCGAT 2 cut(s) 1336, 1992
BsiEI CGRYCG 2 cut(s) 3195, 3257
BsiHKAI GWGCWC 3 cut(s) 556, 916, 1174
BsiHKCI CYCGRG 2 cut(s) 1981, 3590
BsiSI CCGG 6 cut(s) 165, 1862, 1982, 3162, 3257, 3591
BslFI GGGAC 6 cut(s) 31, 865, 2580, 3178, 3288, 3605
BsmAI GTCTC 3 cut(s) 1592, 2523, 3119
BsmFI GGGAC 6 cut(s) 31, 865, 2580, 3178, 3288, 3605
BsmI GAATGC 3 cut(s) 351, 798, 2387
BsoBI CYCGRG 2 cut(s) 1981, 3590
Bsp1286I GDGCHC 4 cut(s) 556, 916, 1174, 2632
BspCNI CTCAG 5 cut(s) 1018, 1219, 1588, 1636, 2533
BspDI ATCGAT 2 cut(s) 1336, 1992
BspFNI CGCG 3 cut(s) 107, 1614, 3152
BspMI ACCTGC 1 cut(s) 3373
BspOI GCTAGC 1 cut(s) 1034
BspQI GCTCTTC 2 cut(s) 2688, 3336
BspT107I GGYRCC 2 cut(s) 155, 2288
BspTI CTTAAG 1 cut(s) 2699
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 5 cut(s) 1065, 1779, 2415, 3009, 3375
BssNI GRCGYC 2 cut(s) 83, 1417
BssSI CACGAG 1 cut(s) 1173
BssT1I CCWWGG 3 cut(s) 2557, 2578, 2965
Bst2BI CACGAG 1 cut(s) 1173
Bst2UI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2286, 2824
Bst4CI ACNGT 5 cut(s) 58, 962, 1744, 2016, 2982
Bst6I CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2688, 3336
BstACI GRCGYC 2 cut(s) 83, 1417
BstAFI CTTAAG 1 cut(s) 2699
BstAPI GCANNNNNTGC 1 cut(s) 2246
BstC8I GCNNGC 9 cut(s) 186, 426, 723, 824, 1008, 1032, 1750, 1872, 1908
BstDEI CTNAG 9 cut(s) 908, 917, 1026, 1066, 1206, 1596, 1644, 2520, 3400
BstEII GGTNACC 1 cut(s) 2074
BstFNI CGCG 3 cut(s) 107, 1614, 3152
BstHHI GCGC 3 cut(s) 109, 217, 2658
BstMAI GTCTC 3 cut(s) 1592, 2523, 3119
BstMCI CGRYCG 2 cut(s) 3195, 3257
BstNI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2286, 2824
BstNSI RCATGY 6 cut(s) 1010, 1079, 1583, 1752, 1910, 2536
BstPI GGTNACC 1 cut(s) 2074
BstSFI CTRYAG 4 cut(s) 675, 2375, 2496, 2913
BstSLI GKGCMC 2 cut(s) 556, 2632
BstUI CGCG 3 cut(s) 107, 1614, 3152
BstV2I GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2559, 3153
BstXI CCANNNNNNTGG 1 cut(s) 3425
BstZI CGGCCG 1 cut(s) 3254
Bsu15I ATCGAT 2 cut(s) 1336, 1992
BsuI GTATCC 2 cut(s) 1661, 1687
BsuTUI ATCGAT 2 cut(s) 1336, 1992
BtgZI GCGATG 1 cut(s) 3195
BtrI CACGTC 1 cut(s) 412
BtsI GCAGTG 3 cut(s) 491, 2058, 3492
BtsIMutI CAGTG 5 cut(s) 491, 1581, 2058, 2398, 3492
BveI ACCTGC 1 cut(s) 3373
Cac8I GCNNGC 9 cut(s) 186, 426, 723, 824, 1008, 1032, 1750, 1872, 1908
CaiI CAGNNNCTG 3 cut(s) 548, 1085, 2912
CfoI GCGC 3 cut(s) 109, 217, 2658
Cfr13I GGNCC 7 cut(s) 46, 201, 821, 2042, 2786, 3302, 3619
Cfr9I CCCGGG 2 cut(s) 1981, 3590
ClaI ATCGAT 2 cut(s) 1336, 1992
CseI GACGC 2 cut(s) 1406, 3158
Csp6I GTAC 8 cut(s) 650, 958, 1220, 1487, 2003, 2189, 2289, 3550
CspCI CAANNNNNGTGG 2 cut(s) 1997, 2032
CviQI GTAC 8 cut(s) 650, 958, 1220, 1487, 2003, 2189, 2289, 3550
DdeI CTNAG 9 cut(s) 908, 917, 1026, 1066, 1206, 1596, 1644, 2520, 3400
DraIII CACNNNGTG 1 cut(s) 560
DrdI GACNNNNNNGTC 1 cut(s) 452
DriI GACNNNNNGTC 2 cut(s) 1416, 3154
DseDI GACNNNNNNGTC 1 cut(s) 452
EaeI YGGCCR 4 cut(s) 528, 1868, 1935, 3254
EagI CGGCCG 1 cut(s) 3254
Eam1104I CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2688, 3336
Eam1105I GACNNNNNGTC 2 cut(s) 1416, 3154
EarI CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2688, 3336
EciI GGCGGA 4 cut(s) 20, 59, 2523, 3339
EclXI CGGCCG 1 cut(s) 3254
Eco130I CCWWGG 3 cut(s) 2557, 2578, 2965
Eco147I AGGCCT 2 cut(s) 1192, 2577
Eco32I GATATC 2 cut(s) 1564, 3544
Eco47I GGWCC 5 cut(s) 46, 201, 2042, 3302, 3619
Eco52I CGGCCG 1 cut(s) 3254
Eco57I CTGAAG 1 cut(s) 3114
Eco88I CYCGRG 2 cut(s) 1981, 3590
Eco91I GGTNACC 1 cut(s) 2074
EcoO109I RGGNCCY 1 cut(s) 3302
EcoO65I GGTNACC 1 cut(s) 2074
EcoRII CCWGG 8 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2284, 2822
EcoRV GATATC 2 cut(s) 1564, 3544
EcoT14I CCWWGG 3 cut(s) 2557, 2578, 2965
EcoT22I ATGCAT 1 cut(s) 2538
ErhI CCWWGG 3 cut(s) 2557, 2578, 2965
FalI AAGNNNNNCTT 4 cut(s) 994, 1026, 1648, 1680
FaqI GGGAC 6 cut(s) 31, 865, 2580, 3178, 3288, 3605
FauI CCCGC 2 cut(s) 831, 2348
FauNDI CATATG 2 cut(s) 331, 742
FbaI TGATCA 2 cut(s) 1822, 2643
FblI GTMKAC 1 cut(s) 432
FspI TGCGCA 1 cut(s) 2657
GlaI GCGC 3 cut(s) 108, 216, 2657
GsaI CCCAGC 4 cut(s) 950, 1248, 2404, 3408
GsuI CTGGAG 5 cut(s) 1107, 1911, 3158, 3288, 3549
HapII CCGG 6 cut(s) 165, 1862, 1982, 3162, 3257, 3591
HgaI GACGC 2 cut(s) 1406, 3158
HhaI GCGC 3 cut(s) 109, 217, 2658
Hin1I GRCGYC 2 cut(s) 83, 1417
Hin6I GCGC 3 cut(s) 107, 215, 2656
HinP1I GCGC 3 cut(s) 107, 215, 2656
HincII GTYRAC 3 cut(s) 445, 1585, 2137
HindII GTYRAC 3 cut(s) 445, 1585, 2137
HindIII AAGCTT 2 cut(s) 681, 723
HpaI GTTAAC 1 cut(s) 1585
HpaII CCGG 6 cut(s) 165, 1862, 1982, 3162, 3257, 3591
HphI GGTGA 6 cut(s) 129, 415, 2468, 2981, 3165, 3354
Hpy166II GTNNAC 8 cut(s) 433, 445, 554, 1421, 1585, 1808, 2137, 3251
Hpy8I GTNNAC 8 cut(s) 433, 445, 554, 1421, 1585, 1808, 2137, 3251
Hpy99I CGWCG 3 cut(s) 85, 88, 1571
HpyAV CCTTC 7 cut(s) 60, 471, 724, 802, 1375, 1383, 3246
HpyCH4III ACNGT 5 cut(s) 58, 962, 1744, 2016, 2982
HpyCH4IV ACGT 3 cut(s) 83, 411, 645
HpyF3I CTNAG 9 cut(s) 908, 917, 1026, 1066, 1206, 1596, 1644, 2520, 3400
HpySE526I ACGT 3 cut(s) 83, 411, 645
Hsp92I GRCGYC 2 cut(s) 83, 1417
HspAI GCGC 3 cut(s) 107, 215, 2656
KflI GGGWCCC 1 cut(s) 3302
KpnI GGTACC 1 cut(s) 2292
Ksp22I TGATCA 2 cut(s) 1822, 2643
KspAI GTTAAC 1 cut(s) 1585
LguI GCTCTTC 2 cut(s) 2688, 3336
LmnI GCTCC 6 cut(s) 173, 348, 751, 2036, 2561, 3052
MaeII ACGT 3 cut(s) 83, 411, 645
MaeIII GTNAC 6 cut(s) 562, 631, 887, 1939, 2074, 3092
MbiI CCGCTC 1 cut(s) 173
MhlI GDGCHC 4 cut(s) 556, 916, 1174, 2632
MlsI TGGCCA 2 cut(s) 530, 1870
MluNI TGGCCA 2 cut(s) 530, 1870
MlyI GAGTC 5 cut(s) 2, 1263, 1537, 1594, 3392
MmeI TCCRAC 1 cut(s) 2014
Mox20I TGGCCA 2 cut(s) 530, 1870
Mph1103I ATGCAT 1 cut(s) 2538
MroXI GAANNNNTTC 2 cut(s) 1474, 1663
MscI TGGCCA 2 cut(s) 530, 1870
MslI CAYNNNNRTG 6 cut(s) 287, 1116, 1753, 2210, 2438, 2667
Msp20I TGGCCA 2 cut(s) 530, 1870
MspA1I CMGCKG 4 cut(s) 2400, 2864, 2912, 3527
MspCI CTTAAG 1 cut(s) 2699
MspI CCGG 6 cut(s) 165, 1862, 1982, 3162, 3257, 3591
Mva1269I GAATGC 3 cut(s) 351, 798, 2387
MvaI CCWGG 8 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2286, 2824
MvnI CGCG 3 cut(s) 107, 1614, 3152
NciI CCSGG 6 cut(s) 165, 1982, 1983, 3163, 3591, 3592
NdeI CATATG 2 cut(s) 331, 742
NheI GCTAGC 1 cut(s) 1030
NmuCI GTSAC 1 cut(s) 1939
NsbI TGCGCA 1 cut(s) 2657
NsiI ATGCAT 1 cut(s) 2538
NspI RCATGY 6 cut(s) 1010, 1079, 1583, 1752, 1910, 2536
PaeI GCATGC 3 cut(s) 1010, 1752, 1910
PaqCI CACCTGC 1 cut(s) 3373
PceI AGGCCT 2 cut(s) 1192, 2577
PciSI GCTCTTC 2 cut(s) 2688, 3336
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 89
PctI GAATGC 3 cut(s) 351, 798, 2387
PdmI GAANNNNTTC 2 cut(s) 1474, 1663
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 2108
PfoI TCCNGGA 3 cut(s) 937, 1236, 3161
PleI GAGTC 5 cut(s) 2, 1262, 1536, 1594, 3391
PpsI GAGTC 5 cut(s) 2, 1262, 1536, 1594, 3391
PpuMI RGGWCCY 1 cut(s) 3302
PshBI ATTAAT 1 cut(s) 1721
Psp5II RGGWCCY 1 cut(s) 3302
Psp6I CCWGG 8 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2284, 2822
PspEI GGTNACC 1 cut(s) 2074
PspFI CCCAGC 4 cut(s) 946, 1244, 2400, 3404
PspGI CCWGG 8 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2284, 2822
PspPI GGNCC 7 cut(s) 46, 201, 821, 2042, 2786, 3302, 3619
PspPPI RGGWCCY 1 cut(s) 3302
PstNI CAGNNNCTG 3 cut(s) 548, 1085, 2912
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 4 cut(s) 2400, 2864, 2912, 3527
RsaI GTAC 8 cut(s) 651, 959, 1221, 1488, 2004, 2190, 2290, 3551
RsaNI GTAC 8 cut(s) 650, 958, 1220, 1487, 2003, 2189, 2289, 3550
RseI CAYNNNNRTG 6 cut(s) 287, 1116, 1753, 2210, 2438, 2667
SapI GCTCTTC 2 cut(s) 2688, 3336
Sau96I GGNCC 7 cut(s) 46, 201, 821, 2042, 2786, 3302, 3619
ScaI AGTACT 1 cut(s) 1488
SchI GAGTC 5 cut(s) 2, 1263, 1537, 1594, 3392
SduI GDGCHC 4 cut(s) 556, 916, 1174, 2632
SfcI CTRYAG 4 cut(s) 675, 2375, 2496, 2913
SinI GGWCC 5 cut(s) 46, 201, 2042, 3302, 3619
SmaI CCCGGG 2 cut(s) 1983, 3592
SmiMI CAYNNNNRTG 6 cut(s) 287, 1116, 1753, 2210, 2438, 2667
SmlI CTYRAG 4 cut(s) 803, 1355, 2424, 2699
SmoI CTYRAG 4 cut(s) 803, 1355, 2424, 2699
SphI GCATGC 3 cut(s) 1010, 1752, 1910
SseBI AGGCCT 2 cut(s) 1192, 2577
SspI AATATT 1 cut(s) 2833
StuI AGGCCT 2 cut(s) 1192, 2577
StyI CCWWGG 3 cut(s) 2557, 2578, 2965
TaaI ACNGT 5 cut(s) 58, 962, 1744, 2016, 2982
TaiI ACGT 3 cut(s) 86, 414, 648
TaqII GACCGA 1 cut(s) 3181
TatI WGTACW 4 cut(s) 1219, 1486, 2002, 2188
TauI GCSGC 4 cut(s) 134, 251, 371, 1541
TscAI CASTG 5 cut(s) 491, 1581, 2065, 2398, 3499
TseFI GTSAC 1 cut(s) 1939
Tsp45I GTSAC 1 cut(s) 1939
TspGWI ACGGA 1 cut(s) 2991
TspMI CCCGGG 2 cut(s) 1981, 3590
TspRI CASTG 5 cut(s) 491, 1581, 2065, 2398, 3499
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 2108
Vha464I CTTAAG 1 cut(s) 2699
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 5 cut(s) 46, 201, 2042, 3302, 3619
VspI ATTAAT 1 cut(s) 1721
XapI RAATTY 8 cut(s) 701, 757, 765, 1017, 2483, 2877, 3020, 3517
XceI RCATGY 6 cut(s) 1010, 1079, 1583, 1752, 1910, 2536
XcmI CCANNNNNNNNNTGG 2 cut(s) 591, 2564
XmaI CCCGGG 2 cut(s) 1981, 3590
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 2 cut(s) 1474, 1663
ZraI GACGTC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 1488
Zsp2I ATGCAT 1 cut(s) 2538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.