Rh4BG001700

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004649
Physical Location & Seq
Forward (+)
297760 .. 323721
25962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG001700.1

Sequence Viewer

Length: 3633 bp
ATGGCGGACTCGTCAGGGACGACCCTGATGGATCTGATAACGGCGGACCCTTCCACAGTATCAGCTTCCACCTCCTCAACGACGTCGTCTTCGTCTTCGTCTTCGTCTTCCGCGCCTCCTCCTCCTCCTCCTTATGCGGCTTCACCCCCACCGCCCAGAGGCACCAGCCCCGGCTCCGCTCTCGGAAAGCCTGCTGTCGAGAAGAGGTCCAAGAGGGCTGCGCTTATGCAGATCCAGAACGACACCATCTCTGCCGCCAAAGCTGCTCTCAATCCCGTTCGCACCAACATCATCATGCCCCAGAAGCACAGGCACAAGCAGAAAAAGCCTGTTTCATATGCTCAACTTGCAAGGAGCATTCACGAACTAGCTGCTTCCTCCGATCAGAAAAGTTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTCGCCGTCTACAATTCTGTTGACCCCTCTGTCGCCCCTTCTCTTCTCATGCTCAATCAGCAGTGTGAGGATAAGAGTGTTCTGCGATATGTCTATTATTACTTGGCCAGGATTTTATCAGATACTGGTGCACAAGGTGTAACCACAGGTGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTGTTGGAGGGGTAACTAGAGCTGATGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAGGCTTCAAATGCTGATGCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCTCCATCAACAAATTCTGAAATTTTGTCCAAATTGTATGAAATTGTGTTTGGCATTCTTGATAAGGTTGCTGATGGCCCGCAAAAACGCAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAAGAGTTTATCATCCGAAGTAACTTGCAATATTCTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTGTCATTTGCTGATCCTGTTGCTGTGAGGCATGCTTTGGAAATTCTGTCTGAGTTAGCCACAAAAGACCCTTATGCAGTTGTGATGGGCTTAGGGAAACATGCAGAGCCTGGAGGGGCTTTGCAGGATGTTCTCCACTTGCATGATGTTCTTGCTAGAGTTGCTCTAGCTAAGTTGTGTTATACAATTTCAAGAGCGAGAGCATTGGATGACAGGACTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTCTGCTTTGAGGCAATCTTATGTATATTGGGAAAACATGATAACACAGAAAGGACTGATGATCGAGCTGCTGGGTGGTACCGATTAACTAGAGAGATTCTCAAGTTGCCAGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGTTCAAAAGACGAGGCGTCCACAGCCCCTTATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCAGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTCGGGCTGCTGCATTTGCTCTAGGCATACAGGATATCGACGAATCAGTGCATGTTAATACATTTTCTGAGACTCTTGATTCACGCGAGATAGATGGTAGTGAAGCCTCACATCCTGAGAGTATCCGAAGAACCTCTTCCTTACCTACAGGGGTAGGTGGCAAGGATACAATAGCTAGTTTGTTAGCTTCATTAATGGAAGTTGTGCGGACAACTGTGGCATGCGAGTGTGTTTATGTTCGAGCAATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCGCATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTTTCTGATCCAGCTTGGCCAGCCACGCTCTTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCCGTTACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCCCCCGGGAAGATCGATGCTGATGTACTACAACTACTGTGGAAGACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGCCTTACATCAGTTGACAGGGTATCTGCATCTGATCCAAAGGCAGCCCTGGCCTTACAGAGATTAGTACAAGCAGCAGTATGGTTCCTTGGAGAAAATGCAAATTATGCTGCTTCTGAATATGCTTGGGAATCTGCAACCCCTCCTGGTACCGCATTGATGATGCTAGATACAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCAGAGGGCGGAGTGCAGTCTCAGTTTTCGGAGATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAGTGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCAAAACTTATCGACATCTATCGCACTAGGCACAATATTAGTCCATCAACTGGTTTGAGTGATCCAGCTGTTGCTACTGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCTGTAGAGCCTGATGCGCTTGATGATGACCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGACGGACTGTTAGGAAATAATGCACCAGCAATGAGCAGGGTTAATGAATTTCTAGCCGGTGCTGGAACTGATGCTCCTGATGTTGATGAGGAAAACATCATCTCTAGGCCGTCAGTTGGTTACGATGATATGTGGGCAAAGACCCTATTAGAGACTTCAGAACTGGAGGAAGAAGACGCGAGGTCATCTGGGACATCTTCACCTGAATCAACAGGCTCGGTCGAAACTTCCATATCATCGCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGATATAGCAATCCATCTATGGGGGGTCCCTCATTCAGTGAGGGTTTAGGATCTCCAATTAGAGAAGAGCCTCCGCCATACTCATCACCTGCAACGCAAAGATTTGAGTCGTTTGAGAATCCCTTAGCCCCCAGATCCCAGAGTTTTGGATCCCAAGATGACGAACGCGTTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACAGCTGGAGAGGAGGTTGATATCGAGTACGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCTGGGAGGGATGGGAAAATGGCTGGTCTGGTCCCAGTCCTCTATGTTAATCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1210

Amino Acids

130.98

Weight (kDa)

5.45

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1154 - 1195 7.2e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3361
AasI GACNNNNNNGTC 1 cut(s) 458
AatII GACGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 2645
Acc36I ACCTGC 1 cut(s) 3361
Acc65I GGTACC 2 cut(s) 1338, 2276
AccB1I GGYRCC 3 cut(s) 161, 1338, 2276
AccB7I CCANNNNNTGG 2 cut(s) 2114, 2834
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 438
AccII CGCG 4 cut(s) 113, 1620, 3140, 3432
AcoI YGGCCR 4 cut(s) 534, 1874, 1941, 3242
AcsI RAATTY 8 cut(s) 707, 763, 771, 1023, 2471, 2865, 3008, 3502
AcuI CTGAAG 1 cut(s) 3102
AcyI GRCGYC 2 cut(s) 83, 1423
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 9 cut(s) 657, 965, 1227, 1340, 1494, 2010, 2196, 2278, 3536
AfiI CCNNNNNNNGG 9 cut(s) 158, 2114, 2534, 2712, 2834, 3077, 3284, 3455, 3580
AflII CTTAAG 1 cut(s) 2687
AflIII ACRYGT 1 cut(s) 3430
AgsI TTSAA 8 cut(s) 693, 736, 977, 1173, 1219, 1412, 1891, 1958
AhdI GACNNNNNGTC 2 cut(s) 1422, 3142
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 9 cut(s) 536, 943, 1090, 1242, 2113, 2175, 2272, 2544, 3574
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AloI GAACNNNNNNTCC 2 cut(s) 3019, 3051
Alw21I GWGCWC 2 cut(s) 562, 922
Alw26I GTCTC 3 cut(s) 1598, 2511, 3107
Alw44I GTGCAC 1 cut(s) 558
AlwNI CAGNNNCTG 3 cut(s) 554, 1091, 2900
Ama87I CYCGRG 1 cut(s) 1988
ApaLI GTGCAC 1 cut(s) 558
ApoI RAATTY 8 cut(s) 707, 763, 771, 1023, 2471, 2865, 3008, 3502
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AseI ATTAAT 1 cut(s) 1727
Asp700I GAANNNNTTC 2 cut(s) 1480, 1669
Asp718I GGTACC 2 cut(s) 1338, 2276
AspLEI GCGC 4 cut(s) 115, 223, 2646, 2917
AspS9I GGNCC 8 cut(s) 46, 207, 827, 2048, 2128, 2774, 3290, 3604
AsuC2I CCSGG 3 cut(s) 171, 1989, 1990
AsuHPI GGTGA 6 cut(s) 135, 421, 2456, 2969, 3153, 3342
AvaI CYCGRG 1 cut(s) 1988
AvaII GGWCC 5 cut(s) 46, 207, 2048, 3290, 3604
BaeGI GKGCMC 2 cut(s) 562, 2620
BalI TGGCCA 2 cut(s) 536, 1876
BamHI GGATCC 4 cut(s) 585, 940, 1246, 3413
BanI GGYRCC 3 cut(s) 161, 1338, 2276
BbsI GAAGAC 9 cut(s) 81, 87, 93, 99, 727, 934, 2033, 2547, 3141
Bbv12I GWGCWC 2 cut(s) 562, 922
BceAI ACGGC 5 cut(s) 57, 419, 1928, 3055, 3257
BcgI CGANNNNNNTGC 4 cut(s) 353, 387, 2548, 2582
BciT130I CCWGG 9 cut(s) 538, 945, 1092, 1244, 2115, 2177, 2274, 2546, 3576
BciVI GTATCC 2 cut(s) 1667, 1693
BclI TGATCA 2 cut(s) 1828, 2631
BcnI CCSGG 3 cut(s) 171, 1989, 1990
BcoDI GTCTC 3 cut(s) 1598, 2511, 3107
BfmI CTRYAG 5 cut(s) 681, 1680, 2363, 2484, 2901
BfrI CTTAAG 1 cut(s) 2687
BfuAI ACCTGC 1 cut(s) 3361
BfuI GTATCC 2 cut(s) 1667, 1693
BglI GCCNNNNNGGC 2 cut(s) 2455, 2773
BglII AGATCT 2 cut(s) 1208, 2438
BmcAI AGTACT 1 cut(s) 1494
Bme18I GGWCC 5 cut(s) 46, 207, 2048, 3290, 3604
BmeRI GACNNNNNGTC 2 cut(s) 1422, 3142
BmeT110I CYCGRG 1 cut(s) 1988
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 8 cut(s) 46, 207, 827, 2048, 2128, 2774, 3290, 3604
BmrI ACTGGG 3 cut(s) 610, 2523, 3602
BmuI ACTGGG 3 cut(s) 610, 2523, 3602
BpiI GAAGAC 9 cut(s) 81, 87, 93, 99, 727, 934, 2033, 2547, 3141
BplI GAGNNNNNCTC 2 cut(s) 1344, 1376
BpmI CTGGAG 5 cut(s) 1113, 1917, 3146, 3276, 3534
Bpu10I CCTNAGC 2 cut(s) 1072, 3388
BpuEI CTTGAG 2 cut(s) 1346, 2397
BpuMI CCSGG 3 cut(s) 171, 1989, 1990
Bsa29I ATCGAT 1 cut(s) 1998
BsaHI GRCGYC 2 cut(s) 83, 1423
BsaXI ACNNNNNCTCC 6 cut(s) 736, 766, 3019, 3049, 3507, 3537
Bsc4I CCNNNNNNNGG 9 cut(s) 158, 2114, 2534, 2712, 2834, 3077, 3284, 3455, 3580
Bse118I RCCGGY 1 cut(s) 3017
Bse1I ACTGG 9 cut(s) 559, 605, 1490, 2322, 2529, 2839, 2866, 3129, 3608
Bse3DI GCAATG 3 cut(s) 1785, 2403, 2997
BseBI CCWGG 9 cut(s) 538, 945, 1092, 1244, 2115, 2177, 2274, 2546, 3576
BseCI ATCGAT 1 cut(s) 1998
BseGI GGATG 9 cut(s) 610, 885, 1114, 1195, 1645, 1809, 2524, 3550, 3589
BseLI CCNNNNNNNGG 9 cut(s) 158, 2114, 2534, 2712, 2834, 3077, 3284, 3455, 3580
BseMI GCAATG 3 cut(s) 1785, 2403, 2997
BseMII CTCAG 5 cut(s) 1023, 1226, 1593, 1641, 2522
BseNI ACTGG 9 cut(s) 559, 605, 1490, 2322, 2529, 2839, 2866, 3129, 3608
BseRI GAGGAG 7 cut(s) 64, 108, 111, 114, 117, 1229, 3533
BseSI GKGCMC 2 cut(s) 562, 2620
BseX3I CGGCCG 1 cut(s) 3242
BseYI CCCAGC 4 cut(s) 952, 1250, 1331, 2388
BsgI GTGCAG 2 cut(s) 1538, 2522
Bsh1236I CGCG 4 cut(s) 113, 1620, 3140, 3432
Bsh1285I CGRYCG 2 cut(s) 3183, 3245
BshNI GGYRCC 3 cut(s) 161, 1338, 2276
BshVI ATCGAT 1 cut(s) 1998
BsiEI CGRYCG 2 cut(s) 3183, 3245
BsiHKAI GWGCWC 2 cut(s) 562, 922
BsiHKCI CYCGRG 1 cut(s) 1988
BsiSI CCGG 4 cut(s) 171, 1989, 3018, 3245
BslFI GGGAC 6 cut(s) 31, 871, 2568, 3166, 3276, 3590
BslI CCNNNNNNNGG 9 cut(s) 158, 2114, 2534, 2712, 2834, 3077, 3284, 3455, 3580
BsmAI GTCTC 3 cut(s) 1598, 2511, 3107
BsmFI GGGAC 6 cut(s) 31, 871, 2568, 3166, 3276, 3590
BsmI GAATGC 3 cut(s) 357, 804, 2375
BsoBI CYCGRG 1 cut(s) 1988
Bsp1286I GDGCHC 3 cut(s) 562, 922, 2620
BspCNI CTCAG 5 cut(s) 1024, 1225, 1594, 1642, 2521
BspDI ATCGAT 1 cut(s) 1998
BspFNI CGCG 4 cut(s) 113, 1620, 3140, 3432
BspMI ACCTGC 1 cut(s) 3361
BspQI GCTCTTC 2 cut(s) 2676, 3324
BspT107I GGYRCC 3 cut(s) 161, 1338, 2276
BspTI CTTAAG 1 cut(s) 2687
BsrBI CCGCTC 1 cut(s) 179
BsrDI GCAATG 3 cut(s) 1785, 2403, 2997
BsrFI RCCGGY 1 cut(s) 3017
BsrI ACTGG 9 cut(s) 559, 605, 1490, 2322, 2529, 2839, 2866, 3129, 3608
BssAI RCCGGY 1 cut(s) 3017
BssNI GRCGYC 2 cut(s) 83, 1423
BssT1I CCWWGG 2 cut(s) 2215, 2953
Bst2UI CCWGG 9 cut(s) 538, 945, 1092, 1244, 2115, 2177, 2274, 2546, 3576
Bst4CI ACNGT 5 cut(s) 58, 968, 1750, 2022, 2970
Bst6I CTCTTC 6 cut(s) 197, 477, 1675, 1956, 2676, 3324
BstACI GRCGYC 2 cut(s) 83, 1423
BstAFI CTTAAG 1 cut(s) 2687
BstAPI GCANNNNNTGC 1 cut(s) 2234
BstC8I GCNNGC 8 cut(s) 192, 432, 729, 830, 1014, 1756, 1878, 1914
BstEII GGTNACC 1 cut(s) 2080
BstF5I GGATG 9 cut(s) 610, 885, 1114, 1195, 1645, 1809, 2524, 3550, 3589
BstFNI CGCG 4 cut(s) 113, 1620, 3140, 3432
BstHHI GCGC 4 cut(s) 115, 223, 2646, 2917
BstMAI GTCTC 3 cut(s) 1598, 2511, 3107
BstMCI CGRYCG 2 cut(s) 3183, 3245
BstNI CCWGG 9 cut(s) 538, 945, 1092, 1244, 2115, 2177, 2274, 2546, 3576
BstNSI RCATGY 5 cut(s) 1016, 1085, 1589, 1758, 1916
BstPI GGTNACC 1 cut(s) 2080
BstSFI CTRYAG 5 cut(s) 681, 1680, 2363, 2484, 2901
BstSLI GKGCMC 2 cut(s) 562, 2620
BstUI CGCG 4 cut(s) 113, 1620, 3140, 3432
BstV2I GAAGAC 9 cut(s) 81, 87, 93, 99, 727, 934, 2033, 2547, 3141
BstXI CCANNNNNNTGG 1 cut(s) 3410
BstZI CGGCCG 1 cut(s) 3242
Bsu15I ATCGAT 1 cut(s) 1998
BsuI GTATCC 2 cut(s) 1667, 1693
BsuTUI ATCGAT 1 cut(s) 1998
BtgZI GCGATG 1 cut(s) 3183
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 9 cut(s) 610, 885, 1114, 1195, 1645, 1809, 2524, 3550, 3589
BtsI GCAGTG 3 cut(s) 497, 2064, 3477
BtsIMutI CAGTG 6 cut(s) 497, 1587, 2064, 2386, 3307, 3477
BveI ACCTGC 1 cut(s) 3361
Cac8I GCNNGC 8 cut(s) 192, 432, 729, 830, 1014, 1756, 1878, 1914
CaiI CAGNNNCTG 3 cut(s) 554, 1091, 2900
CfoI GCGC 4 cut(s) 115, 223, 2646, 2917
Cfr10I RCCGGY 1 cut(s) 3017
Cfr13I GGNCC 8 cut(s) 46, 207, 827, 2048, 2128, 2774, 3290, 3604
Cfr9I CCCGGG 1 cut(s) 1988
ClaI ATCGAT 1 cut(s) 1998
CseI GACGC 2 cut(s) 1412, 3146
Csp6I GTAC 9 cut(s) 656, 964, 1226, 1339, 1493, 2009, 2195, 2277, 3535
CspCI CAANNNNNGTGG 2 cut(s) 2003, 2038
CviQI GTAC 9 cut(s) 656, 964, 1226, 1339, 1493, 2009, 2195, 2277, 3535
DraIII CACNNNGTG 1 cut(s) 566
DrdI GACNNNNNNGTC 1 cut(s) 458
DriI GACNNNNNGTC 2 cut(s) 1422, 3142
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 4 cut(s) 534, 1874, 1941, 3242
EagI CGGCCG 1 cut(s) 3242
Eam1104I CTCTTC 6 cut(s) 197, 477, 1675, 1956, 2676, 3324
Eam1105I GACNNNNNGTC 2 cut(s) 1422, 3142
EarI CTCTTC 6 cut(s) 197, 477, 1675, 1956, 2676, 3324
EciI GGCGGA 4 cut(s) 20, 59, 2511, 3327
EclXI CGGCCG 1 cut(s) 3242
Eco130I CCWWGG 2 cut(s) 2215, 2953
Eco147I AGGCCT 1 cut(s) 2565
Eco32I GATATC 2 cut(s) 1570, 3529
Eco47I GGWCC 5 cut(s) 46, 207, 2048, 3290, 3604
Eco52I CGGCCG 1 cut(s) 3242
Eco57I CTGAAG 1 cut(s) 3102
Eco88I CYCGRG 1 cut(s) 1988
Eco91I GGTNACC 1 cut(s) 2080
EcoO109I RGGNCCY 1 cut(s) 3290
EcoO65I GGTNACC 1 cut(s) 2080
EcoRII CCWGG 9 cut(s) 536, 943, 1090, 1242, 2113, 2175, 2272, 2544, 3574
EcoRV GATATC 2 cut(s) 1570, 3529
EcoT14I CCWWGG 2 cut(s) 2215, 2953
EcoT22I ATGCAT 1 cut(s) 2526
ErhI CCWWGG 2 cut(s) 2215, 2953
FalI AAGNNNNNCTT 2 cut(s) 1654, 1686
FaqI GGGAC 6 cut(s) 31, 871, 2568, 3166, 3276, 3590
FauI CCCGC 2 cut(s) 837, 2336
FauNDI CATATG 2 cut(s) 337, 748
FbaI TGATCA 2 cut(s) 1828, 2631
FblI GTMKAC 1 cut(s) 438
FokI GGATG 9 cut(s) 617, 872, 1121, 1202, 1632, 1816, 2511, 3557, 3596
FspI TGCGCA 1 cut(s) 2645
GlaI GCGC 4 cut(s) 114, 222, 2645, 2916
GsaI CCCAGC 4 cut(s) 956, 1254, 1335, 2392
GsuI CTGGAG 5 cut(s) 1113, 1917, 3146, 3276, 3534
HapII CCGG 4 cut(s) 171, 1989, 3018, 3245
HgaI GACGC 2 cut(s) 1412, 3146
HhaI GCGC 4 cut(s) 115, 223, 2646, 2917
Hin1I GRCGYC 2 cut(s) 83, 1423
Hin6I GCGC 4 cut(s) 113, 221, 2644, 2915
HinP1I GCGC 4 cut(s) 113, 221, 2644, 2915
HincII GTYRAC 2 cut(s) 451, 2143
HindII GTYRAC 2 cut(s) 451, 2143
HindIII AAGCTT 1 cut(s) 729
HpaII CCGG 4 cut(s) 171, 1989, 3018, 3245
HphI GGTGA 6 cut(s) 135, 421, 2456, 2969, 3153, 3342
Hpy166II GTNNAC 6 cut(s) 439, 451, 560, 1427, 2143, 3239
Hpy8I GTNNAC 6 cut(s) 439, 451, 560, 1427, 2143, 3239
Hpy99I CGWCG 3 cut(s) 85, 88, 1577
HpyAV CCTTC 6 cut(s) 60, 477, 730, 1381, 1389, 3234
HpyCH4III ACNGT 5 cut(s) 58, 968, 1750, 2022, 2970
HpyCH4IV ACGT 2 cut(s) 83, 417
HpySE526I ACGT 2 cut(s) 83, 417
Hsp92I GRCGYC 2 cut(s) 83, 1423
HspAI GCGC 4 cut(s) 113, 221, 2644, 2915
KflI GGGWCCC 1 cut(s) 3290
KpnI GGTACC 2 cut(s) 1342, 2280
Ksp22I TGATCA 2 cut(s) 1828, 2631
LguI GCTCTTC 2 cut(s) 2676, 3324
LmnI GCTCC 6 cut(s) 179, 354, 757, 2042, 2549, 3040
MaeII ACGT 2 cut(s) 83, 417
MaeIII GTNAC 6 cut(s) 568, 637, 893, 1945, 2080, 3080
MbiI CCGCTC 1 cut(s) 179
MhlI GDGCHC 3 cut(s) 562, 922, 2620
MlsI TGGCCA 2 cut(s) 536, 1876
MluI ACGCGT 1 cut(s) 3430
MluNI TGGCCA 2 cut(s) 536, 1876
MlyI GAGTC 5 cut(s) 2, 1269, 1543, 1600, 3380
MmeI TCCRAC 2 cut(s) 610, 2020
Mox20I TGGCCA 2 cut(s) 536, 1876
Mph1103I ATGCAT 1 cut(s) 2526
MroXI GAANNNNTTC 2 cut(s) 1480, 1669
MscI TGGCCA 2 cut(s) 536, 1876
MslI CAYNNNNRTG 5 cut(s) 293, 1122, 1759, 2426, 2655
Msp20I TGGCCA 2 cut(s) 536, 1876
MspA1I CMGCKG 4 cut(s) 2388, 2852, 2900, 3512
MspCI CTTAAG 1 cut(s) 2687
MspI CCGG 4 cut(s) 171, 1989, 3018, 3245
Mva1269I GAATGC 3 cut(s) 357, 804, 2375
MvaI CCWGG 9 cut(s) 538, 945, 1092, 1244, 2115, 2177, 2274, 2546, 3576
MvnI CGCG 4 cut(s) 113, 1620, 3140, 3432
NciI CCSGG 3 cut(s) 171, 1989, 1990
NdeI CATATG 2 cut(s) 337, 748
NsbI TGCGCA 1 cut(s) 2645
NsiI ATGCAT 1 cut(s) 2526
NspI RCATGY 5 cut(s) 1016, 1085, 1589, 1758, 1916
PaeI GCATGC 3 cut(s) 1016, 1758, 1916
PaqCI CACCTGC 1 cut(s) 3361
PasI CCCWGGG 1 cut(s) 3575
PceI AGGCCT 1 cut(s) 2565
PciSI GCTCTTC 2 cut(s) 2676, 3324
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 89
PctI GAATGC 3 cut(s) 357, 804, 2375
PdmI GAANNNNTTC 2 cut(s) 1480, 1669
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 2 cut(s) 2114, 2834
PfoI TCCNGGA 2 cut(s) 943, 1242
PleI GAGTC 5 cut(s) 2, 1268, 1542, 1600, 3379
PpsI GAGTC 5 cut(s) 2, 1268, 1542, 1600, 3379
PpuMI RGGWCCY 1 cut(s) 3290
PshBI ATTAAT 1 cut(s) 1727
Psp5II RGGWCCY 1 cut(s) 3290
Psp6I CCWGG 9 cut(s) 536, 943, 1090, 1242, 2113, 2175, 2272, 2544, 3574
PspEI GGTNACC 1 cut(s) 2080
PspFI CCCAGC 4 cut(s) 952, 1250, 1331, 2388
PspGI CCWGG 9 cut(s) 536, 943, 1090, 1242, 2113, 2175, 2272, 2544, 3574
PspPI GGNCC 8 cut(s) 46, 207, 827, 2048, 2128, 2774, 3290, 3604
PspPPI RGGWCCY 1 cut(s) 3290
PstNI CAGNNNCTG 3 cut(s) 554, 1091, 2900
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 4 cut(s) 2388, 2852, 2900, 3512
RsaI GTAC 9 cut(s) 657, 965, 1227, 1340, 1494, 2010, 2196, 2278, 3536
RsaNI GTAC 9 cut(s) 656, 964, 1226, 1339, 1493, 2009, 2195, 2277, 3535
RseI CAYNNNNRTG 5 cut(s) 293, 1122, 1759, 2426, 2655
SapI GCTCTTC 2 cut(s) 2676, 3324
Sau96I GGNCC 8 cut(s) 46, 207, 827, 2048, 2128, 2774, 3290, 3604
ScaI AGTACT 1 cut(s) 1494
SchI GAGTC 5 cut(s) 2, 1269, 1543, 1600, 3380
SduI GDGCHC 3 cut(s) 562, 922, 2620
SfcI CTRYAG 5 cut(s) 681, 1680, 2363, 2484, 2901
SinI GGWCC 5 cut(s) 46, 207, 2048, 3290, 3604
SmaI CCCGGG 1 cut(s) 1990
SmiMI CAYNNNNRTG 5 cut(s) 293, 1122, 1759, 2426, 2655
SmlI CTYRAG 3 cut(s) 1361, 2412, 2687
SmoI CTYRAG 3 cut(s) 1361, 2412, 2687
SphI GCATGC 3 cut(s) 1016, 1758, 1916
SseBI AGGCCT 1 cut(s) 2565
SspI AATATT 2 cut(s) 905, 2821
StuI AGGCCT 1 cut(s) 2565
StyI CCWWGG 2 cut(s) 2215, 2953
TaaI ACNGT 5 cut(s) 58, 968, 1750, 2022, 2970
TaiI ACGT 2 cut(s) 86, 420
TaqI TCGA 9 cut(s) 198, 1324, 1572, 1774, 1998, 2094, 2796, 3183, 3531
TaqII GACCGA 1 cut(s) 3169
TatI WGTACW 4 cut(s) 1225, 1492, 2008, 2194
TauI GCSGC 2 cut(s) 140, 257
TscAI CASTG 6 cut(s) 497, 1587, 2071, 2386, 3307, 3484
TspGWI ACGGA 1 cut(s) 2979
TspMI CCCGGG 1 cut(s) 1988
TspRI CASTG 6 cut(s) 497, 1587, 2071, 2386, 3307, 3484
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 2 cut(s) 2114, 2834
Vha464I CTTAAG 1 cut(s) 2687
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 5 cut(s) 46, 207, 2048, 3290, 3604
VspI ATTAAT 1 cut(s) 1727
XapI RAATTY 8 cut(s) 707, 763, 771, 1023, 2471, 2865, 3008, 3502
XceI RCATGY 5 cut(s) 1016, 1085, 1589, 1758, 1916
XcmI CCANNNNNNNNNTGG 2 cut(s) 597, 2552
XmaI CCCGGG 1 cut(s) 1988
XmiI GTMKAC 1 cut(s) 438
XmnI GAANNNNTTC 2 cut(s) 1480, 1669
ZraI GACGTC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 1494
Zsp2I ATGCAT 1 cut(s) 2526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.