Rh4AG001900

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004649
Physical Location & Seq
Forward (+)
298841 .. 318880
20040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG001900.1

Sequence Viewer

Length: 3627 bp
ATGGCGGACTCGTCAGGGACGACCCTGATGGATCTGATAACGGCGGACCCTTCCACAGTATCAGCTTCCACCTCCTCAACGACGTCGTCTTCGTCTTCGTCTTCCGCGCCTCCTCCTCCTCCTCCTTATGCGGCTTCACCCCCACCGCCCAGAGGCACCAGCCCCGGCTCCGCTCTCGGAAAGCCTGCTGTCGAGAAGAGGTCCAAGAGGGCTGCGCTTATGCAGATCCAGAACGACACCATCTCTGCCGCCAAAGCTGCTCTCAATCCCGTTCGCACCAACATCATCATGCCCCAGAAGCACAGGCACAAGCAGAAAAAGCCTGTTTCATATGCTCAACTTGCAAGGAGCATTCACGAACTTGCTGCTTCCTCCGATCAGAAAAGTTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTCGCCGTCTACAATTCTGTTGACCCCTCTGTCGCCCCTTCTCTTCTCATGCTCAATCAGCAGTGTGAGGATAAGAGTGTTCTGCGATATGTCTATTATTACTTGGCCAGGATTTTATCAGATACTGGTGCACAAGGTGTAACCACAGGTGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTGTTGGAGGGGTAACTAGAGCTGATGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAGGCTTCAAATGCTGATGCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCTCCATCAACAAATTCTGAAATTTTGTCCAAATTGTATGAAATTGTGTTTGGCATTCTTGATAAGGTTGCTGATGGCCCGCAAAAACGCAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAAGAGTTTATCATCCGAAGTAACTTGCAATATGCTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTGTCATTTGCTGATCCTGTTGCTGTGAGGCATGCTTTGGAAATTCTGTCTGAGTTAGCCACAAAAGACCCTTATGCAGTTGTGATGGGCTTAGGGAAACATGCAGAGCCTGGAGGGGCTTTGCAGGATGTTCTCCACTTGCATGATGTTCTTGCTAGAGTTGCTCTAGCTAAGTTGTGTTATACAATTTCAAGAGCGAGAGCATTGGATGACAGGCCTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTCTGCTTTGAGGCAATCTTATGTATATTGGGAAAACATGATAACACAGAAAGGACTGATGATCGAGCTGCTGGGTGGTACCGATTAACTAGAGAGATTCTCAAGTTGCCAGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGTTCAAAAGACGAGGCGTCCACAGCCCCTTATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCAGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTCGGGCTGCTGCATTTGCTCTAGGCATACAGGATATCGACGAATCAGTGCATGTTAATACATTTTCTGAGACTCTTGATTCACGCGAGATAGATGGTAGTGAAGCCTCACATCCTGAGAGTATCCGAAGAACCTCTTCCTTACCTACAGGGGTAGGTGGCAAGGATACAATAGCTAGTTTGTTAGCTTCATTAATGGAAGTTGTCCGGACAACTGTGGCATGCGAGTGTGTTTATGTTCGAGCAATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCGCATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTTTCTGATCCAGCTTGGCCAGCCACGCTCTTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCCGTTACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCCCCCGGGAAGATCGATGCTGATGTACTACAACTACTGTGGAAGACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGCCTTACATCAGTTGACAGGGTATCTGCATCTGATCCAAAGGCAGCCCTGGCCTTACAGAGATTAGTACAAGCAGCAGTATGGTTCCTTGGAGAAAATGCAAATTATGCTGCTTCTGAATATGCTTGGGAATCTGCAACCCCTCCTGGTACTGCATTGATGATGCTAGATACAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCAGAGGGCGGAGTGCAGTCTCAGTTTTCTGAGATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAGTGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCAAAACTTATCGACATCTATCGCACTAGGCACAATATTAGTCCATCAACTGGTTTGAGTGATCCAGCTGTTGCTACTGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCTGTAGAGCCTGATGCGCTTGATGATGATCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGACGGACTGTTAGGAAATAATGCACCAGCAATGAGCAGGGTTAATGAATTTCTAGCCGGTGCTGGAACTGATGCTCCTGATGTTGATGAGGAAAACATCATCTCTAGGCCGTCAGTTGGTTACGATGATATGTGGGCAAAGACCCTATTAGAGACTTCAGAACTGGAGGAAGAAGACGCGAGGTCATCTGGGACATCTTCACCTGAATCAACAGGCTCGGTCGAAACTTCCATATCATCGCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGAAATAGCAATCCATCTATGGGGGGTCCCTCATTCAGTGAGGGTTTAGGATCTCCAATTAGAGAAGAGCCTCCGCCATACTCGTCACCTGCAATGCAAAGCTTTGAGTCGTTTGAGAATCCCTTAGCCCCCAGATCCCAGAGTTTTGGATCCCAAGATGACGAACGCGTTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACAGCTGGAGAGGAGGTTGATATCGAGTACGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCTGGGAGGGATGGGAAAATGGCTGGTCTGGTCCCAGTCCTCTATGTTAATCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1208

Amino Acids

130.69

Weight (kDa)

5.42

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1152 - 1193 7.2e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3355
AasI GACNNNNNNGTC 1 cut(s) 452
AatII GACGTC 1 cut(s) 86
Acc16I TGCGCA 1 cut(s) 2639
Acc36I ACCTGC 1 cut(s) 3355
Acc65I GGTACC 1 cut(s) 1332
AccB1I GGYRCC 2 cut(s) 155, 1332
AccB7I CCANNNNNTGG 2 cut(s) 2108, 2828
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 432
AccII CGCG 4 cut(s) 107, 1614, 3134, 3426
AccIII TCCGGA 1 cut(s) 1734
AcoI YGGCCR 4 cut(s) 528, 1868, 1935, 3236
AcsI RAATTY 8 cut(s) 701, 757, 765, 1017, 2465, 2859, 3002, 3496
AcuI CTGAAG 1 cut(s) 3096
AcyI GRCGYC 2 cut(s) 83, 1417
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 9 cut(s) 651, 959, 1221, 1334, 1488, 2004, 2190, 2272, 3530
AfiI CCNNNNNNNGG 9 cut(s) 152, 2108, 2528, 2706, 2828, 3071, 3278, 3449, 3574
AflII CTTAAG 1 cut(s) 2681
AflIII ACRYGT 1 cut(s) 3424
AgsI TTSAA 8 cut(s) 687, 730, 971, 1167, 1213, 1406, 1885, 1952
AhdI GACNNNNNGTC 2 cut(s) 1416, 3136
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 9 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2266, 2538, 3568
AjuI GAANNNNNNNTTGG 2 cut(s) 777, 809
AloI GAACNNNNNNTCC 2 cut(s) 3013, 3045
Alw21I GWGCWC 2 cut(s) 556, 916
Alw26I GTCTC 3 cut(s) 1592, 2505, 3101
Alw44I GTGCAC 1 cut(s) 552
AlwNI CAGNNNCTG 3 cut(s) 548, 1085, 2894
Ama87I CYCGRG 1 cut(s) 1982
Aor13HI TCCGGA 1 cut(s) 1734
ApaLI GTGCAC 1 cut(s) 552
ApoI RAATTY 8 cut(s) 701, 757, 765, 1017, 2465, 2859, 3002, 3496
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AseI ATTAAT 1 cut(s) 1721
Asp700I GAANNNNTTC 2 cut(s) 1474, 1663
Asp718I GGTACC 1 cut(s) 1332
AspLEI GCGC 4 cut(s) 109, 217, 2640, 2911
AspS9I GGNCC 8 cut(s) 46, 201, 821, 2042, 2122, 2768, 3284, 3598
AsuC2I CCSGG 3 cut(s) 165, 1983, 1984
AsuHPI GGTGA 6 cut(s) 129, 415, 2450, 2963, 3147, 3336
AvaI CYCGRG 1 cut(s) 1982
AvaII GGWCC 5 cut(s) 46, 201, 2042, 3284, 3598
BaeGI GKGCMC 2 cut(s) 556, 2614
BalI TGGCCA 2 cut(s) 530, 1870
BamHI GGATCC 4 cut(s) 579, 934, 1240, 3407
BanI GGYRCC 2 cut(s) 155, 1332
BbsI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2541, 3135
Bbv12I GWGCWC 2 cut(s) 556, 916
BceAI ACGGC 5 cut(s) 57, 413, 1922, 3049, 3251
BcgI CGANNNNNNTGC 4 cut(s) 347, 381, 2542, 2576
BciT130I CCWGG 9 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2268, 2540, 3570
BciVI GTATCC 2 cut(s) 1661, 1687
BclI TGATCA 2 cut(s) 1822, 2625
BcnI CCSGG 3 cut(s) 165, 1983, 1984
BcoDI GTCTC 3 cut(s) 1592, 2505, 3101
BfmI CTRYAG 5 cut(s) 675, 1674, 2357, 2478, 2895
BfrI CTTAAG 1 cut(s) 2681
BfuAI ACCTGC 1 cut(s) 3355
BfuI GTATCC 2 cut(s) 1661, 1687
BglI GCCNNNNNGGC 2 cut(s) 2449, 2767
BglII AGATCT 2 cut(s) 1202, 2432
BmcAI AGTACT 1 cut(s) 1488
Bme18I GGWCC 5 cut(s) 46, 201, 2042, 3284, 3598
BmeRI GACNNNNNGTC 2 cut(s) 1416, 3136
BmeT110I CYCGRG 1 cut(s) 1982
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 8 cut(s) 46, 201, 821, 2042, 2122, 2768, 3284, 3598
BmrI ACTGGG 3 cut(s) 604, 2517, 3596
BmuI ACTGGG 3 cut(s) 604, 2517, 3596
BpiI GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2541, 3135
BplI GAGNNNNNCTC 2 cut(s) 1338, 1370
BpmI CTGGAG 5 cut(s) 1107, 1911, 3140, 3270, 3528
Bpu10I CCTNAGC 2 cut(s) 1066, 3382
BpuEI CTTGAG 2 cut(s) 1340, 2391
BpuMI CCSGG 3 cut(s) 165, 1983, 1984
Bsa29I ATCGAT 1 cut(s) 1992
BsaBI GATNNNNATC 1 cut(s) 2919
BsaHI GRCGYC 2 cut(s) 83, 1417
BsaWI WCCGGW 1 cut(s) 1734
BsaXI ACNNNNNCTCC 6 cut(s) 730, 760, 3013, 3043, 3501, 3531
Bsc4I CCNNNNNNNGG 9 cut(s) 152, 2108, 2528, 2706, 2828, 3071, 3278, 3449, 3574
Bse118I RCCGGY 1 cut(s) 3011
Bse1I ACTGG 9 cut(s) 553, 599, 1484, 2316, 2523, 2833, 2860, 3123, 3602
Bse3DI GCAATG 4 cut(s) 1779, 2397, 2991, 3357
Bse8I GATNNNNATC 1 cut(s) 2919
BseAI TCCGGA 1 cut(s) 1734
BseBI CCWGG 9 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2268, 2540, 3570
BseCI ATCGAT 1 cut(s) 1992
BseGI GGATG 9 cut(s) 604, 879, 1108, 1189, 1639, 1803, 2518, 3544, 3583
BseJI GATNNNNATC 1 cut(s) 2919
BseLI CCNNNNNNNGG 9 cut(s) 152, 2108, 2528, 2706, 2828, 3071, 3278, 3449, 3574
BseMI GCAATG 4 cut(s) 1779, 2397, 2991, 3357
BseMII CTCAG 6 cut(s) 1017, 1220, 1587, 1635, 2502, 2516
BseNI ACTGG 9 cut(s) 553, 599, 1484, 2316, 2523, 2833, 2860, 3123, 3602
BseRI GAGGAG 7 cut(s) 64, 102, 105, 108, 111, 1223, 3527
BseSI GKGCMC 2 cut(s) 556, 2614
BseX3I CGGCCG 1 cut(s) 3236
BseYI CCCAGC 4 cut(s) 946, 1244, 1325, 2382
BsgI GTGCAG 2 cut(s) 1532, 2516
Bsh1236I CGCG 4 cut(s) 107, 1614, 3134, 3426
Bsh1285I CGRYCG 2 cut(s) 3177, 3239
BshNI GGYRCC 2 cut(s) 155, 1332
BshVI ATCGAT 1 cut(s) 1992
BsiEI CGRYCG 2 cut(s) 3177, 3239
BsiHKAI GWGCWC 2 cut(s) 556, 916
BsiHKCI CYCGRG 1 cut(s) 1982
BsiSI CCGG 5 cut(s) 165, 1735, 1983, 3012, 3239
BslFI GGGAC 6 cut(s) 31, 865, 2562, 3160, 3270, 3584
BslI CCNNNNNNNGG 9 cut(s) 152, 2108, 2528, 2706, 2828, 3071, 3278, 3449, 3574
BsmAI GTCTC 3 cut(s) 1592, 2505, 3101
BsmFI GGGAC 6 cut(s) 31, 865, 2562, 3160, 3270, 3584
BsmI GAATGC 3 cut(s) 351, 798, 2369
BsoBI CYCGRG 1 cut(s) 1982
Bsp1286I GDGCHC 3 cut(s) 556, 916, 2614
Bsp13I TCCGGA 1 cut(s) 1734
BspCNI CTCAG 6 cut(s) 1018, 1219, 1588, 1636, 2503, 2515
BspDI ATCGAT 1 cut(s) 1992
BspEI TCCGGA 1 cut(s) 1734
BspFNI CGCG 4 cut(s) 107, 1614, 3134, 3426
BspMI ACCTGC 1 cut(s) 3355
BspQI GCTCTTC 2 cut(s) 2670, 3318
BspT107I GGYRCC 2 cut(s) 155, 1332
BspTI CTTAAG 1 cut(s) 2681
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 4 cut(s) 1779, 2397, 2991, 3357
BsrFI RCCGGY 1 cut(s) 3011
BsrI ACTGG 9 cut(s) 553, 599, 1484, 2316, 2523, 2833, 2860, 3123, 3602
BssAI RCCGGY 1 cut(s) 3011
BssNI GRCGYC 2 cut(s) 83, 1417
BssT1I CCWWGG 2 cut(s) 2209, 2947
Bst2UI CCWGG 9 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2268, 2540, 3570
Bst4CI ACNGT 5 cut(s) 58, 962, 1744, 2016, 2964
Bst6I CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2670, 3318
BstACI GRCGYC 2 cut(s) 83, 1417
BstAFI CTTAAG 1 cut(s) 2681
BstAPI GCANNNNNTGC 1 cut(s) 2228
BstC8I GCNNGC 8 cut(s) 186, 426, 723, 824, 1008, 1750, 1872, 1908
BstEII GGTNACC 1 cut(s) 2074
BstF5I GGATG 9 cut(s) 604, 879, 1108, 1189, 1639, 1803, 2518, 3544, 3583
BstFNI CGCG 4 cut(s) 107, 1614, 3134, 3426
BstHHI GCGC 4 cut(s) 109, 217, 2640, 2911
BstMAI GTCTC 3 cut(s) 1592, 2505, 3101
BstMCI CGRYCG 2 cut(s) 3177, 3239
BstNI CCWGG 9 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2268, 2540, 3570
BstNSI RCATGY 5 cut(s) 1010, 1079, 1583, 1752, 1910
BstPI GGTNACC 1 cut(s) 2074
BstSFI CTRYAG 5 cut(s) 675, 1674, 2357, 2478, 2895
BstSLI GKGCMC 2 cut(s) 556, 2614
BstUI CGCG 4 cut(s) 107, 1614, 3134, 3426
BstV2I GAAGAC 8 cut(s) 81, 87, 93, 721, 928, 2027, 2541, 3135
BstXI CCANNNNNNTGG 1 cut(s) 3404
BstZI CGGCCG 1 cut(s) 3236
Bsu15I ATCGAT 1 cut(s) 1992
BsuI GTATCC 2 cut(s) 1661, 1687
BsuTUI ATCGAT 1 cut(s) 1992
BtgZI GCGATG 1 cut(s) 3177
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 9 cut(s) 604, 879, 1108, 1189, 1639, 1803, 2518, 3544, 3583
BtsI GCAGTG 3 cut(s) 491, 2058, 3471
BtsIMutI CAGTG 6 cut(s) 491, 1581, 2058, 2380, 3301, 3471
BveI ACCTGC 1 cut(s) 3355
Cac8I GCNNGC 8 cut(s) 186, 426, 723, 824, 1008, 1750, 1872, 1908
CaiI CAGNNNCTG 3 cut(s) 548, 1085, 2894
CfoI GCGC 4 cut(s) 109, 217, 2640, 2911
Cfr10I RCCGGY 1 cut(s) 3011
Cfr13I GGNCC 8 cut(s) 46, 201, 821, 2042, 2122, 2768, 3284, 3598
Cfr9I CCCGGG 1 cut(s) 1982
ClaI ATCGAT 1 cut(s) 1992
CseI GACGC 2 cut(s) 1406, 3140
Csp6I GTAC 9 cut(s) 650, 958, 1220, 1333, 1487, 2003, 2189, 2271, 3529
CspCI CAANNNNNGTGG 2 cut(s) 1997, 2032
CviQI GTAC 9 cut(s) 650, 958, 1220, 1333, 1487, 2003, 2189, 2271, 3529
DraIII CACNNNGTG 1 cut(s) 560
DrdI GACNNNNNNGTC 1 cut(s) 452
DriI GACNNNNNGTC 2 cut(s) 1416, 3136
DseDI GACNNNNNNGTC 1 cut(s) 452
EaeI YGGCCR 4 cut(s) 528, 1868, 1935, 3236
EagI CGGCCG 1 cut(s) 3236
Eam1104I CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2670, 3318
Eam1105I GACNNNNNGTC 2 cut(s) 1416, 3136
EarI CTCTTC 6 cut(s) 191, 471, 1669, 1950, 2670, 3318
EciI GGCGGA 4 cut(s) 20, 59, 2505, 3321
EclXI CGGCCG 1 cut(s) 3236
Eco130I CCWWGG 2 cut(s) 2209, 2947
Eco147I AGGCCT 2 cut(s) 1192, 2559
Eco32I GATATC 2 cut(s) 1564, 3523
Eco47I GGWCC 5 cut(s) 46, 201, 2042, 3284, 3598
Eco52I CGGCCG 1 cut(s) 3236
Eco57I CTGAAG 1 cut(s) 3096
Eco88I CYCGRG 1 cut(s) 1982
Eco91I GGTNACC 1 cut(s) 2074
EcoO109I RGGNCCY 1 cut(s) 3284
EcoO65I GGTNACC 1 cut(s) 2074
EcoRII CCWGG 9 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2266, 2538, 3568
EcoRV GATATC 2 cut(s) 1564, 3523
EcoT14I CCWWGG 2 cut(s) 2209, 2947
EcoT22I ATGCAT 1 cut(s) 2520
ErhI CCWWGG 2 cut(s) 2209, 2947
FalI AAGNNNNNCTT 2 cut(s) 1648, 1680
FaqI GGGAC 6 cut(s) 31, 865, 2562, 3160, 3270, 3584
FauI CCCGC 2 cut(s) 831, 2330
FauNDI CATATG 2 cut(s) 331, 742
FbaI TGATCA 2 cut(s) 1822, 2625
FblI GTMKAC 1 cut(s) 432
FokI GGATG 9 cut(s) 611, 866, 1115, 1196, 1626, 1810, 2505, 3551, 3590
FspI TGCGCA 1 cut(s) 2639
GlaI GCGC 4 cut(s) 108, 216, 2639, 2910
GsaI CCCAGC 4 cut(s) 950, 1248, 1329, 2386
GsuI CTGGAG 5 cut(s) 1107, 1911, 3140, 3270, 3528
HapII CCGG 5 cut(s) 165, 1735, 1983, 3012, 3239
HgaI GACGC 2 cut(s) 1406, 3140
HhaI GCGC 4 cut(s) 109, 217, 2640, 2911
Hin1I GRCGYC 2 cut(s) 83, 1417
Hin6I GCGC 4 cut(s) 107, 215, 2638, 2909
HinP1I GCGC 4 cut(s) 107, 215, 2638, 2909
HincII GTYRAC 2 cut(s) 445, 2137
HindII GTYRAC 2 cut(s) 445, 2137
HindIII AAGCTT 3 cut(s) 723, 3358, 3621
HpaII CCGG 5 cut(s) 165, 1735, 1983, 3012, 3239
HphI GGTGA 6 cut(s) 129, 415, 2450, 2963, 3147, 3336
Hpy166II GTNNAC 6 cut(s) 433, 445, 554, 1421, 2137, 3233
Hpy8I GTNNAC 6 cut(s) 433, 445, 554, 1421, 2137, 3233
Hpy99I CGWCG 3 cut(s) 85, 88, 1571
HpyAV CCTTC 6 cut(s) 60, 471, 724, 1375, 1383, 3228
HpyCH4III ACNGT 5 cut(s) 58, 962, 1744, 2016, 2964
HpyCH4IV ACGT 2 cut(s) 83, 411
HpySE526I ACGT 2 cut(s) 83, 411
Hsp92I GRCGYC 2 cut(s) 83, 1417
HspAI GCGC 4 cut(s) 107, 215, 2638, 2909
KflI GGGWCCC 1 cut(s) 3284
Kpn2I TCCGGA 1 cut(s) 1734
KpnI GGTACC 1 cut(s) 1336
Ksp22I TGATCA 2 cut(s) 1822, 2625
LguI GCTCTTC 2 cut(s) 2670, 3318
LmnI GCTCC 6 cut(s) 173, 348, 751, 2036, 2543, 3034
MaeII ACGT 2 cut(s) 83, 411
MaeIII GTNAC 7 cut(s) 562, 631, 887, 1939, 2074, 3074, 3342
MbiI CCGCTC 1 cut(s) 173
MhlI GDGCHC 3 cut(s) 556, 916, 2614
MlsI TGGCCA 2 cut(s) 530, 1870
MluI ACGCGT 1 cut(s) 3424
MluNI TGGCCA 2 cut(s) 530, 1870
MlyI GAGTC 5 cut(s) 2, 1263, 1537, 1594, 3374
MmeI TCCRAC 2 cut(s) 604, 2014
Mox20I TGGCCA 2 cut(s) 530, 1870
Mph1103I ATGCAT 1 cut(s) 2520
MroI TCCGGA 1 cut(s) 1734
MroXI GAANNNNTTC 2 cut(s) 1474, 1663
MscI TGGCCA 2 cut(s) 530, 1870
MslI CAYNNNNRTG 5 cut(s) 287, 1116, 1753, 2420, 2649
Msp20I TGGCCA 2 cut(s) 530, 1870
MspA1I CMGCKG 4 cut(s) 2382, 2846, 2894, 3506
MspCI CTTAAG 1 cut(s) 2681
MspI CCGG 5 cut(s) 165, 1735, 1983, 3012, 3239
Mva1269I GAATGC 3 cut(s) 351, 798, 2369
MvaI CCWGG 9 cut(s) 532, 939, 1086, 1238, 2109, 2171, 2268, 2540, 3570
MvnI CGCG 4 cut(s) 107, 1614, 3134, 3426
NciI CCSGG 3 cut(s) 165, 1983, 1984
NdeI CATATG 2 cut(s) 331, 742
NmuCI GTSAC 1 cut(s) 3342
NsbI TGCGCA 1 cut(s) 2639
NsiI ATGCAT 1 cut(s) 2520
NspI RCATGY 5 cut(s) 1010, 1079, 1583, 1752, 1910
PaeI GCATGC 3 cut(s) 1010, 1752, 1910
PaqCI CACCTGC 1 cut(s) 3355
PasI CCCWGGG 1 cut(s) 3569
PceI AGGCCT 2 cut(s) 1192, 2559
PciSI GCTCTTC 2 cut(s) 2670, 3318
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 89
PctI GAATGC 3 cut(s) 351, 798, 2369
PdmI GAANNNNTTC 2 cut(s) 1474, 1663
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 2 cut(s) 2108, 2828
PfoI TCCNGGA 2 cut(s) 937, 1236
PleI GAGTC 5 cut(s) 2, 1262, 1536, 1594, 3373
PpsI GAGTC 5 cut(s) 2, 1262, 1536, 1594, 3373
PpuMI RGGWCCY 1 cut(s) 3284
PshBI ATTAAT 1 cut(s) 1721
Psp5II RGGWCCY 1 cut(s) 3284
Psp6I CCWGG 9 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2266, 2538, 3568
PspEI GGTNACC 1 cut(s) 2074
PspFI CCCAGC 4 cut(s) 946, 1244, 1325, 2382
PspGI CCWGG 9 cut(s) 530, 937, 1084, 1236, 2107, 2169, 2266, 2538, 3568
PspPI GGNCC 8 cut(s) 46, 201, 821, 2042, 2122, 2768, 3284, 3598
PspPPI RGGWCCY 1 cut(s) 3284
PstNI CAGNNNCTG 3 cut(s) 548, 1085, 2894
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 4 cut(s) 2382, 2846, 2894, 3506
RsaI GTAC 9 cut(s) 651, 959, 1221, 1334, 1488, 2004, 2190, 2272, 3530
RsaNI GTAC 9 cut(s) 650, 958, 1220, 1333, 1487, 2003, 2189, 2271, 3529
RseI CAYNNNNRTG 5 cut(s) 287, 1116, 1753, 2420, 2649
SapI GCTCTTC 2 cut(s) 2670, 3318
Sau96I GGNCC 8 cut(s) 46, 201, 821, 2042, 2122, 2768, 3284, 3598
ScaI AGTACT 1 cut(s) 1488
SchI GAGTC 5 cut(s) 2, 1263, 1537, 1594, 3374
SduI GDGCHC 3 cut(s) 556, 916, 2614
SfcI CTRYAG 5 cut(s) 675, 1674, 2357, 2478, 2895
SinI GGWCC 5 cut(s) 46, 201, 2042, 3284, 3598
SmaI CCCGGG 1 cut(s) 1984
SmiMI CAYNNNNRTG 5 cut(s) 287, 1116, 1753, 2420, 2649
SmlI CTYRAG 3 cut(s) 1355, 2406, 2681
SmoI CTYRAG 3 cut(s) 1355, 2406, 2681
SphI GCATGC 3 cut(s) 1010, 1752, 1910
SseBI AGGCCT 2 cut(s) 1192, 2559
SspI AATATT 1 cut(s) 2815
StuI AGGCCT 2 cut(s) 1192, 2559
StyI CCWWGG 2 cut(s) 2209, 2947
TaaI ACNGT 5 cut(s) 58, 962, 1744, 2016, 2964
TaiI ACGT 2 cut(s) 86, 414
TaqI TCGA 9 cut(s) 192, 1318, 1566, 1768, 1992, 2088, 2790, 3177, 3525
TaqII GACCGA 1 cut(s) 3163
TatI WGTACW 4 cut(s) 1219, 1486, 2002, 2188
TauI GCSGC 2 cut(s) 134, 251
TscAI CASTG 6 cut(s) 491, 1581, 2065, 2380, 3301, 3478
TseFI GTSAC 1 cut(s) 3342
Tsp45I GTSAC 1 cut(s) 3342
TspGWI ACGGA 1 cut(s) 2973
TspMI CCCGGG 1 cut(s) 1982
TspRI CASTG 6 cut(s) 491, 1581, 2065, 2380, 3301, 3478
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 2 cut(s) 2108, 2828
Vha464I CTTAAG 1 cut(s) 2681
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 5 cut(s) 46, 201, 2042, 3284, 3598
VspI ATTAAT 1 cut(s) 1721
XapI RAATTY 8 cut(s) 701, 757, 765, 1017, 2465, 2859, 3002, 3496
XceI RCATGY 5 cut(s) 1010, 1079, 1583, 1752, 1910
XcmI CCANNNNNNNNNTGG 2 cut(s) 591, 2546
XmaI CCCGGG 1 cut(s) 1982
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 2 cut(s) 1474, 1663
ZraI GACGTC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 1488
Zsp2I ATGCAT 1 cut(s) 2520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.