Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

5407043 .. 5415553

Gene Structure 8511 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g06000.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGTGGAGGCTGAAAGTTTCAGAAGGAGGAAGCGATGGTAGTGATCGACGGCTCACAAGTGTGAATAATCACATCGGAAGACAGTACTGGGAGTTCTACCGGAATCTCGGAACACCGGAAGAGAGAGCTCAGGTGGAAGAAGCTCGGCTTGAGTTTCGTAACAATCGTTTTCGATCCAAACATAGTTCCGATCTGTTAATGAGACTTCAGTTTGCGAAGGAGAAAGAAAGTGATCGAATGGAGCTACCAGCTCAGGTGAAAGTGAAAAGTGAAGACGAAATTACTGAGGAAGTAGTAACAACCACGTTGAGGAGGGGGTTGAAATTTTACTCGACTCTTCAGACTGAGGATGGTTTCTGGCCGGGTGATTACGGCGGTCCTTTGTTTCTTCTACCTGGCTTGGTTATTGGATTAGCAGTAACAGGCGCTCTTGATGCAATCTTGTCACACCAGCATCACAAGGAGATCTGCCGCTATATTTACAACCATCAGAACAAAGATGGAGGTTGGGGACTGCATATAGAAGGGCCAAGCACAATGTTTTGTACAGCTCTATCCTATGTGAGTCTGAGACTGCTCGGAGAGGAGATGGACGGCGGCGACGATGGCGCCATGGAGAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCAGCATCCCATCTTGGGGAAAGATGTGGCTTTCAGTACTTGGTGTATATGAATGGGATGGTAACAATCCACTGCCTCCTGAATTGTGGCTTTTACCTGATTTTCTTCCAGTACACCCAGGTCGAATGTGGTGTCACTGTCGGATGGTATACCTGCCTATGTCATATTTATACGGCAAGAGATTTGTTGGTCCTATCAATGGCCTTATTTTATCCCTCAGAAGAGAGCTTTATACCTTTCCCTATCACCTCACTGATTGGGATCAGGCAAGAAATCTATGTGCCAAGGAGGATTTGTATTACCCACATCCCATTTTACAGGACGTACTGTGGGGGTCTCTTCATAAGGTTGGGGAACCTCTTCTCATGAGATGGCCATTTTCTAAGCTCAGGCAGAAAGCTCTCACCACTGTAATGGAACACATTCACTATGAGGATGAAAACACTCAGTATATCTGTATTGGACCTGTCAATAAGGTACTAAACATGATCTGTTGTTGGGTGGAGGATCCGAATTCAGAAGCATACAAGTGTCATCTTTTGAGAATAAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAACGGATCTCAACTCTGGGATGTTGCTTTGGCTGTTCAAGCGATCCTTTCTACCAACCTTGTGAATGAATATGGTTCCGTGCTTAAGAAGGCAAACGGTTTCATCAAAAACACACAAGTCAGGAGAGACAGCTATGGCGATCTTAGTTACTGGTACCGTCACATTTCTAAGGGAGGATGGCCCTTTTCTACTCCAGACAATGGCTGGCCTGTCTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTCATTATCACGTATGCCCCGGGAAATTGTGGGAGAAGCAATAGCAGCTGATCAACTGCATGATGCTGTTAATGTGGTATTATCCCTACAGAACAGCAATGGAGGGTTTGCATCATATGAGCTCACAAGATCCTATCCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCATTATTGATTATCAGTACGTGGAATGCACATCAGCAGCAATCAAAGGCCTTAAAGCATTCATGAATTTATATCCATGGCATCGGAAGAAAGAAATAGAAGCGTGCATTGGAAAGGCAGTTCAGTTCATCGAGAGCATACAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGTTTAGTGGCTAGTGGCAAGACCTACCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTATCCAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTCCTTTCATGCCAAAACAAGGTATACACAAATCTAGAAGGAAACAAACCACATATAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGCGAAAGGGATCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAAATGAAGAACGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAGAAATTGCATGATTAGTTACTCCGCTTACAGAAATATCTTCCCAATCTGGGCACTTGGAGAGTATCGAAGCCGGGTATTGGTACCTCACAAATCATCATTCAAAAGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

772

Amino Acids

88.07

Weight (kDa)

7.82

Isoelectric Point (pI)

45.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	144 - 397	1.6e-37	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	420 - 757	7.4e-53	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	816
Acc65I	GGTACC	2 cut(s)	1404, 2284
AccB1I	GGYRCC	3 cut(s)	608, 1404, 2284
AccB7I	CCANNNNNTGG	1 cut(s)	1451
AccI	GTMKAC	2 cut(s)	804, 2019
AciI	CCGC	4 cut(s)	375, 472, 597, 2226
AcoI	YGGCCR	2 cut(s)	359, 1028
AcsI	RAATTY	3 cut(s)	323, 1168, 1744
AcuI	CTGAAG	3 cut(s)	191, 323, 1500
AcyI	GRCGYC	1 cut(s)	609
AfiI	CCNNNNNNNGG	9 cut(s)	309, 670, 671, 854, 1451, 1638, 2014, 2251, 2281
AflII	CTTAAG	1 cut(s)	1334
AgsI	TTSAA	6 cut(s)	322, 1289, 1489, 2087, 2200, 2305
AjnI	CCWGG	3 cut(s)	394, 772, 1637
AjuI	GAANNNNNNNTTGG	2 cut(s)	1770, 1802
AleI	CACNNNNGTG	1 cut(s)	59
AloI	GAACNNNNNNTCC	2 cut(s)	1782, 1814
Alw21I	GWGCWC	2 cut(s)	130, 1623
Alw26I	GTCTC	5 cut(s)	196, 565, 996, 1371, 1468
Ama87I	CYCGRG	1 cut(s)	1518
AoxI	GGCC	7 cut(s)	359, 527, 856, 1028, 1430, 1457, 1726
ApeKI	GCWGC	4 cut(s)	1493, 1544, 1715, 2129
ApoI	RAATTY	3 cut(s)	323, 1168, 1744
ArsI	GACNNNNNNTTYG	2 cut(s)	772, 804
Asp700I	GAANNNNTTC	2 cut(s)	1014, 1077
Asp718I	GGTACC	2 cut(s)	1404, 2284
AspLEI	GCGC	2 cut(s)	428, 611
AspS9I	GGNCC	5 cut(s)	377, 527, 845, 1118, 1431
AsuC2I	CCSGG	4 cut(s)	363, 1519, 1520, 2276
AsuHPI	GGTGA	5 cut(s)	268, 377, 632, 893, 1051
AvaI	CYCGRG	1 cut(s)	1518
AvaII	GGWCC	3 cut(s)	377, 845, 1118
BaeGI	GKGCMC	1 cut(s)	2257
BalI	TGGCCA	1 cut(s)	1030
BamHI	GGATCC	2 cut(s)	1162, 2107
BanI	GGYRCC	3 cut(s)	608, 1404, 2284
BanII	GRGCYC	2 cut(s)	130, 1623
BbsI	GAAGAC	3 cut(s)	85, 279, 2188
Bbv12I	GWGCWC	2 cut(s)	130, 1623
BbvI	GCAGC	4 cut(s)	1505, 1556, 1727, 2141
BceAI	ACGGC	4 cut(s)	65, 388, 610, 844
BcgI	CGANNNNNNTGC	2 cut(s)	2101, 2135
BciT130I	CCWGG	3 cut(s)	396, 774, 1639
BclI	TGATCA	3 cut(s)	1549, 1650, 2142
BcnI	CCSGG	4 cut(s)	363, 1519, 1520, 2276
BcoDI	GTCTC	5 cut(s)	196, 565, 996, 1371, 1468
BfaI	CTAG	2 cut(s)	1902, 2030
BfmI	CTRYAG	1 cut(s)	1586
BfoI	RGCGCY	2 cut(s)	429, 612
BfrI	CTTAAG	1 cut(s)	1334
BfuAI	ACCTGC	1 cut(s)	816
BglII	AGATCT	1 cut(s)	465
BisI	GCNGC	6 cut(s)	472, 598, 1494, 1545, 1716, 2130
BlsI	GCNGC	6 cut(s)	473, 599, 1495, 1546, 1717, 2131
BmcAI	AGTACT	2 cut(s)	86, 693
Bme1390I	CCNGG	7 cut(s)	363, 396, 774, 1519, 1520, 1639, 2276
Bme18I	GGWCC	3 cut(s)	377, 845, 1118
BmeT110I	CYCGRG	1 cut(s)	1518
BmgT120I	GGNCC	5 cut(s)	377, 527, 845, 1118, 1431
BmiI	GGNNCC	7 cut(s)	610, 1011, 1164, 1327, 1406, 2109, 2286
BmrFI	CCNGG	7 cut(s)	363, 396, 774, 1519, 1520, 1639, 2276
BmrI	ACTGGG	1 cut(s)	97
BmsI	GCATC	7 cut(s)	424, 463, 669, 1552, 1619, 1768, 2131
BmuI	ACTGGG	1 cut(s)	97
BpiI	GAAGAC	3 cut(s)	85, 279, 2188
BpmI	CTGGAG	1 cut(s)	1428
Bpu10I	CCTNAGC	3 cut(s)	129, 252, 1043
BpuEI	CTTGAG	1 cut(s)	170
BpuMI	CCSGG	4 cut(s)	363, 1519, 1520, 2276
BsaAI	YACGTR	2 cut(s)	1511, 1699
BsaBI	GATNNNNATC	1 cut(s)	915
BsaHI	GRCGYC	1 cut(s)	609
BsaI	GGTCTC	1 cut(s)	996
BsaJI	CCNNGG	8 cut(s)	612, 643, 772, 939, 1517, 1518, 1637, 1754
BsaWI	WCCGGW	2 cut(s)	99, 115
BsaXI	ACNNNNNCTCC	2 cut(s)	935, 965
Bsc4I	CCNNNNNNNGG	9 cut(s)	309, 670, 671, 854, 1451, 1638, 2014, 2251, 2281
Bse1I	ACTGG	4 cut(s)	92, 764, 1406, 2065
Bse3DI	GCAATG	1 cut(s)	1603
Bse8I	GATNNNNATC	1 cut(s)	915
BseBI	CCWGG	3 cut(s)	396, 774, 1639
BseDI	CCNNGG	8 cut(s)	612, 643, 772, 939, 1517, 1518, 1637, 1754
BseGI	GGATG	9 cut(s)	355, 660, 718, 804, 961, 1096, 1276, 1433, 2069
BseJI	GATNNNNATC	1 cut(s)	915
BseLI	CCNNNNNNNGG	9 cut(s)	309, 670, 671, 854, 1451, 1638, 2014, 2251, 2281
BseMI	GCAATG	1 cut(s)	1603
BseMII	CTCAG	9 cut(s)	143, 266, 276, 336, 560, 886, 1057, 1115, 1479
BseNI	ACTGG	4 cut(s)	92, 764, 1406, 2065
BseRI	GAGGAG	3 cut(s)	325, 599, 663
BseSI	GKGCMC	1 cut(s)	2257
BseXI	GCAGC	4 cut(s)	1505, 1556, 1727, 2141
BseYI	CCCAGC	1 cut(s)	1983
BshFI	GGCC	7 cut(s)	361, 529, 858, 1030, 1432, 1459, 1728
BshNI	GGYRCC	3 cut(s)	608, 1404, 2284
BsiHKAI	GWGCWC	2 cut(s)	130, 1623
BsiHKCI	CYCGRG	1 cut(s)	1518
BsiSI	CCGG	5 cut(s)	100, 116, 362, 1519, 2275
BslFI	GGGAC	1 cut(s)	525
BslI	CCNNNNNNNGG	9 cut(s)	309, 670, 671, 854, 1451, 1638, 2014, 2251, 2281
BsmAI	GTCTC	5 cut(s)	196, 565, 996, 1371, 1468
BsmFI	GGGAC	1 cut(s)	525
BsmI	GAATGC	2 cut(s)	1709, 1736
BsnI	GGCC	7 cut(s)	361, 529, 858, 1030, 1432, 1459, 1728
Bso31I	GGTCTC	1 cut(s)	996
BsoBI	CYCGRG	1 cut(s)	1518
Bsp1286I	GDGCHC	3 cut(s)	130, 1623, 2257
Bsp1407I	TGTACA	2 cut(s)	545, 1472
Bsp19I	CCATGG	2 cut(s)	612, 1754
BspACI	CCGC	4 cut(s)	375, 472, 597, 2226
BspANI	GGCC	7 cut(s)	361, 529, 858, 1030, 1432, 1459, 1728
BspCNI	CTCAG	9 cut(s)	142, 265, 277, 337, 561, 885, 1056, 1114, 1478
BspHI	TCATGA	2 cut(s)	1020, 1740
BspLI	GGNNCC	7 cut(s)	610, 1011, 1164, 1327, 1406, 2109, 2286
BspMI	ACCTGC	1 cut(s)	816
BspQI	GCTCTTC	1 cut(s)	1933
BspT107I	GGYRCC	3 cut(s)	608, 1404, 2284
BspTI	CTTAAG	1 cut(s)	1334
BspTNI	GGTCTC	1 cut(s)	996
BsrDI	GCAATG	1 cut(s)	1603
BsrGI	TGTACA	2 cut(s)	545, 1472
BsrI	ACTGG	4 cut(s)	92, 764, 1406, 2065
BssECI	CCNNGG	8 cut(s)	612, 643, 772, 939, 1517, 1518, 1637, 1754
BssNAI	GTATAC	2 cut(s)	805, 2020
BssNI	GRCGYC	1 cut(s)	609
BssT1I	CCWWGG	3 cut(s)	612, 939, 1754
Bst1107I	GTATAC	2 cut(s)	805, 2020
Bst2UI	CCWGG	3 cut(s)	396, 774, 1639
Bst4CI	ACNGT	7 cut(s)	84, 644, 794, 984, 1066, 1349, 1409
Bst6I	CTCTTC	6 cut(s)	114, 342, 871, 999, 1020, 1933
BstACI	GRCGYC	1 cut(s)	609
BstAFI	CTTAAG	1 cut(s)	1334
BstAUI	TGTACA	2 cut(s)	545, 1472
BstBAI	YACGTR	2 cut(s)	1511, 1699
BstC8I	GCNNGC	2 cut(s)	1457, 1783
BstDSI	CCRYGG	3 cut(s)	612, 643, 1754
BstF5I	GGATG	9 cut(s)	355, 660, 718, 804, 961, 1096, 1276, 1433, 2069
BstH2I	RGCGCY	2 cut(s)	429, 612
BstHHI	GCGC	2 cut(s)	428, 611
BstMAI	GTCTC	5 cut(s)	196, 565, 996, 1371, 1468
BstMWI	GCNNNNNNNGC	6 cut(s)	434, 606, 1289, 1544, 1963, 2075
BstNI	CCWGG	3 cut(s)	396, 774, 1639
BstNSI	RCATGY	1 cut(s)	1947
BstSCI	CCNGG	7 cut(s)	361, 394, 772, 1517, 1518, 1637, 2274
BstSFI	CTRYAG	1 cut(s)	1586
BstSLI	GKGCMC	1 cut(s)	2257
BstV1I	GCAGC	4 cut(s)	1505, 1556, 1727, 2141
BstV2I	GAAGAC	3 cut(s)	85, 279, 2188
BstX2I	RGATCY	5 cut(s)	465, 1162, 1256, 1628, 2107
BstXI	CCANNNNNNTGG	1 cut(s)	1069
BstYI	RGATCY	5 cut(s)	465, 1162, 1256, 1628, 2107
BstZ17I	GTATAC	2 cut(s)	805, 2020
BsuRI	GGCC	7 cut(s)	361, 529, 858, 1030, 1432, 1459, 1728
BtgI	CCRYGG	3 cut(s)	612, 643, 1754
BtgZI	GCGATG	1 cut(s)	48
BtsCI	GGATG	9 cut(s)	355, 660, 718, 804, 961, 1096, 1276, 1433, 2069
BtsI	GCAGTG	1 cut(s)	725
BtsIMutI	CAGTG	5 cut(s)	725, 790, 906, 1062, 2058
BveI	ACCTGC	1 cut(s)	816
Cac8I	GCNNGC	2 cut(s)	1457, 1783
CciI	TCATGA	2 cut(s)	1020, 1740
CfoI	GCGC	2 cut(s)	428, 611
Cfr13I	GGNCC	5 cut(s)	377, 527, 845, 1118, 1431
Cfr9I	CCCGGG	1 cut(s)	1518
DinI	GGCGCC	1 cut(s)	610
EaeI	YGGCCR	2 cut(s)	359, 1028
Eam1104I	CTCTTC	6 cut(s)	114, 342, 871, 999, 1020, 1933
EarI	CTCTTC	6 cut(s)	114, 342, 871, 999, 1020, 1933
Ecl136II	GAGCTC	2 cut(s)	128, 1621
Eco130I	CCWWGG	3 cut(s)	612, 939, 1754
Eco147I	AGGCCT	1 cut(s)	1728
Eco24I	GRGCYC	2 cut(s)	130, 1623
Eco31I	GGTCTC	1 cut(s)	996
Eco32I	GATATC	1 cut(s)	1678
Eco47I	GGWCC	3 cut(s)	377, 845, 1118
Eco53kI	GAGCTC	2 cut(s)	128, 1621
Eco57I	CTGAAG	3 cut(s)	191, 323, 1500
Eco88I	CYCGRG	1 cut(s)	1518
EcoICRI	GAGCTC	2 cut(s)	128, 1621
EcoRI	GAATTC	1 cut(s)	1168
EcoRII	CCWGG	3 cut(s)	394, 772, 1637
EcoRV	GATATC	1 cut(s)	1678
EcoT14I	CCWWGG	3 cut(s)	612, 939, 1754
EcoT38I	GRGCYC	2 cut(s)	130, 1623
EgeI	GGCGCC	1 cut(s)	610
EheI	GGCGCC	1 cut(s)	610
ErhI	CCWWGG	3 cut(s)	612, 939, 1754
FalI	AAGNNNNNCTT	4 cut(s)	132, 164, 1281, 1313
FaqI	GGGAC	1 cut(s)	525
FauNDI	CATATG	1 cut(s)	1615
FbaI	TGATCA	3 cut(s)	1549, 1650, 2142
FblI	GTMKAC	2 cut(s)	804, 2019
Fnu4HI	GCNGC	6 cut(s)	472, 598, 1494, 1545, 1716, 2130
FokI	GGATG	9 cut(s)	362, 647, 725, 811, 948, 1103, 1283, 1440, 2076
FriOI	GRGCYC	2 cut(s)	130, 1623
Fsp4HI	GCNGC	6 cut(s)	472, 598, 1494, 1545, 1716, 2130
FspBI	CTAG	2 cut(s)	1902, 2030
GlaI	GCGC	2 cut(s)	427, 610
GluI	GCNGC	6 cut(s)	472, 598, 1494, 1545, 1716, 2130
GsaI	CCCAGC	1 cut(s)	1987
GsuI	CTGGAG	1 cut(s)	1428
HaeII	RGCGCY	2 cut(s)	429, 612
HaeIII	GGCC	7 cut(s)	361, 529, 858, 1030, 1432, 1459, 1728
HapII	CCGG	5 cut(s)	100, 116, 362, 1519, 2275
HhaI	GCGC	2 cut(s)	428, 611
Hin1I	GRCGYC	1 cut(s)	609
Hin6I	GCGC	2 cut(s)	426, 609
HinP1I	GCGC	2 cut(s)	426, 609
HinfI	GANTC	6 cut(s)	103, 334, 565, 634, 1973, 2193
HpaII	CCGG	5 cut(s)	100, 116, 362, 1519, 2275
HphI	GGTGA	5 cut(s)	268, 377, 632, 893, 1051
Hpy166II	GTNNAC	4 cut(s)	769, 805, 2020, 2056
Hpy188III	TCNNGA	9 cut(s)	431, 734, 1021, 1372, 1445, 1741, 1810, 1826, 2030
Hpy8I	GTNNAC	4 cut(s)	769, 805, 2020, 2056
Hpy99I	CGWCG	2 cut(s)	51, 605
HpyAV	CCTTC	8 cut(s)	17, 211, 518, 613, 1333, 1475, 1483, 2027
HpyCH4III	ACNGT	7 cut(s)	84, 644, 794, 984, 1066, 1349, 1409
HpyCH4IV	ACGT	4 cut(s)	305, 978, 1510, 1698
HpyF10VI	GCNNNNNNNGC	6 cut(s)	434, 606, 1289, 1544, 1963, 2075
HpySE526I	ACGT	4 cut(s)	305, 978, 1510, 1698
Hsp92I	GRCGYC	1 cut(s)	609
HspAI	GCGC	2 cut(s)	426, 609
KasI	GGCGCC	1 cut(s)	608
KpnI	GGTACC	2 cut(s)	1408, 2288
Ksp22I	TGATCA	3 cut(s)	1549, 1650, 2142
LguI	GCTCTTC	1 cut(s)	1933
LmnI	GCTCC	1 cut(s)	241
Lsp1109I	GCAGC	4 cut(s)	1505, 1556, 1727, 2141
LweI	GCATC	7 cut(s)	424, 463, 669, 1552, 1619, 1768, 2131
MaeI	CTAG	2 cut(s)	1902, 2030
MaeII	ACGT	4 cut(s)	305, 978, 1510, 1698
MflI	RGATCY	5 cut(s)	465, 1162, 1256, 1628, 2107
MhlI	GDGCHC	3 cut(s)	130, 1623, 2257
MlsI	TGGCCA	1 cut(s)	1030
MluCI	AATT	9 cut(s)	279, 323, 737, 1168, 1524, 1744, 2082, 2146, 2206
MluNI	TGGCCA	1 cut(s)	1030
Mly113I	GGCGCC	1 cut(s)	609
MlyI	GAGTC	3 cut(s)	328, 574, 2202
MmeI	TCCRAC	2 cut(s)	776, 1623
Mox20I	TGGCCA	1 cut(s)	1030
MroXI	GAANNNNTTC	2 cut(s)	1014, 1077
MscI	TGGCCA	1 cut(s)	1030
MseI	TTAA	4 cut(s)	197, 1335, 1569, 1731
MslI	CAYNNNNRTG	3 cut(s)	59, 1067, 1183
Msp20I	TGGCCA	1 cut(s)	1030
MspA1I	CMGCKG	3 cut(s)	1478, 1547, 1661
MspCI	CTTAAG	1 cut(s)	1334
MspI	CCGG	5 cut(s)	100, 116, 362, 1519, 2275
MspR9I	CCNGG	7 cut(s)	363, 396, 774, 1519, 1520, 1639, 2276
Mva1269I	GAATGC	2 cut(s)	1709, 1736
MvaI	CCWGG	3 cut(s)	396, 774, 1639
MwoI	GCNNNNNNNGC	6 cut(s)	434, 606, 1289, 1544, 1963, 2075
NarI	GGCGCC	1 cut(s)	609
NciI	CCSGG	4 cut(s)	363, 1519, 1520, 2276
NcoI	CCATGG	2 cut(s)	612, 1754
NdeI	CATATG	1 cut(s)	1615
NlaIV	GGNNCC	7 cut(s)	610, 1011, 1164, 1327, 1406, 2109, 2286
NmeAIII	GCCGAG	1 cut(s)	124
NmuCI	GTSAC	3 cut(s)	444, 788, 1409
NspI	RCATGY	1 cut(s)	1947
OliI	CACNNNNGTG	1 cut(s)	59
PagI	TCATGA	2 cut(s)	1020, 1740
PceI	AGGCCT	1 cut(s)	1728
PciSI	GCTCTTC	1 cut(s)	1933
PcsI	WCGNNNNNNNCGW	2 cut(s)	163, 600
PctI	GAATGC	2 cut(s)	1709, 1736
PdmI	GAANNNNTTC	2 cut(s)	1014, 1077
PfeI	GAWTC	3 cut(s)	103, 634, 1973
PflMI	CCANNNNNTGG	1 cut(s)	1451
PkrI	GCNGC	6 cut(s)	473, 599, 1495, 1546, 1717, 2131
PleI	GAGTC	3 cut(s)	328, 573, 2201
PluTI	GGCGCC	1 cut(s)	612
PpsI	GAGTC	3 cut(s)	328, 573, 2201
Ppu21I	YACGTR	2 cut(s)	1511, 1699
Psp124BI	GAGCTC	2 cut(s)	130, 1623
Psp6I	CCWGG	3 cut(s)	394, 772, 1637
PspFI	CCCAGC	1 cut(s)	1983
PspGI	CCWGG	3 cut(s)	394, 772, 1637
PspN4I	GGNNCC	7 cut(s)	610, 1011, 1164, 1327, 1406, 2109, 2286
PspPI	GGNCC	5 cut(s)	377, 527, 845, 1118, 1431
PsrI	GAACNNNNNNTAC	2 cut(s)	77, 109
PsuI	RGATCY	5 cut(s)	465, 1162, 1256, 1628, 2107
PvuII	CAGCTG	3 cut(s)	1478, 1547, 1661
RseI	CAYNNNNRTG	3 cut(s)	59, 1067, 1183
SacI	GAGCTC	2 cut(s)	130, 1623
SapI	GCTCTTC	1 cut(s)	1933
SaqAI	TTAA	4 cut(s)	197, 1335, 1569, 1731
SatI	GCNGC	6 cut(s)	472, 598, 1494, 1545, 1716, 2130
Sau96I	GGNCC	5 cut(s)	377, 527, 845, 1118, 1431
ScaI	AGTACT	2 cut(s)	86, 693
SchI	GAGTC	3 cut(s)	328, 574, 2202
ScrFI	CCNGG	7 cut(s)	363, 396, 774, 1519, 1520, 1639, 2276
SduI	GDGCHC	3 cut(s)	130, 1623, 2257
SfaNI	GCATC	7 cut(s)	424, 463, 669, 1552, 1619, 1768, 2131
SfcI	CTRYAG	1 cut(s)	1586
SfoI	GGCGCC	1 cut(s)	610
SinI	GGWCC	3 cut(s)	377, 845, 1118
SmaI	CCCGGG	1 cut(s)	1520
SmiMI	CAYNNNNRTG	3 cut(s)	59, 1067, 1183
SmlI	CTYRAG	2 cut(s)	149, 1334
SmoI	CTYRAG	2 cut(s)	149, 1334
Sse9I	AATT	9 cut(s)	279, 323, 737, 1168, 1524, 1744, 2082, 2146, 2206
SseBI	AGGCCT	1 cut(s)	1728
SsiI	CCGC	4 cut(s)	375, 472, 597, 2226
SspDI	GGCGCC	1 cut(s)	608
SspMI	CTAG	2 cut(s)	1902, 2030
SstI	GAGCTC	2 cut(s)	130, 1623
StuI	AGGCCT	1 cut(s)	1728
StyD4I	CCNGG	7 cut(s)	361, 394, 772, 1517, 1518, 1637, 2274
StyI	CCWWGG	3 cut(s)	612, 939, 1754
TaaI	ACNGT	7 cut(s)	84, 644, 794, 984, 1066, 1349, 1409
TaiI	ACGT	4 cut(s)	308, 981, 1513, 1701
TaqI	TCGA	9 cut(s)	46, 172, 235, 332, 778, 1809, 1971, 2125, 2269
TasI	AATT	9 cut(s)	279, 323, 737, 1168, 1524, 1744, 2082, 2146, 2206
TatI	WGTACW	5 cut(s)	84, 545, 691, 766, 1472
TauI	GCSGC	2 cut(s)	474, 600
TfiI	GAWTC	3 cut(s)	103, 634, 1973
Tru1I	TTAA	4 cut(s)	197, 1335, 1569, 1731
Tru9I	TTAA	4 cut(s)	197, 1335, 1569, 1731
TscAI	CASTG	5 cut(s)	732, 797, 913, 1069, 2065
TseFI	GTSAC	3 cut(s)	444, 788, 1409
TseI	GCWGC	4 cut(s)	1493, 1544, 1715, 2129
Tsp45I	GTSAC	3 cut(s)	444, 788, 1409
TspGWI	ACGGA	3 cut(s)	1269, 1318, 2178
TspMI	CCCGGG	1 cut(s)	1518
TspRI	CASTG	5 cut(s)	732, 797, 913, 1069, 2065
Van91I	CCANNNNNTGG	1 cut(s)	1451
Vha464I	CTTAAG	1 cut(s)	1334
VpaK11BI	GGWCC	3 cut(s)	377, 845, 1118
XapI	RAATTY	3 cut(s)	323, 1168, 1744
XbaI	TCTAGA	1 cut(s)	2029
XceI	RCATGY	1 cut(s)	1947
XcmI	CCANNNNNNNNNTGG	2 cut(s)	780, 1452
XmaI	CCCGGG	1 cut(s)	1518
XmiI	GTMKAC	2 cut(s)	804, 2019
XmnI	GAANNNNTTC	2 cut(s)	1014, 1077
XspI	CTAG	2 cut(s)	1902, 2030
ZrmI	AGTACT	2 cut(s)	86, 693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.