Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

13171774 .. 13188810

Gene Structure 17037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G006360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAACAGATGGTAATCCATGGCTCACAAGTGTGAATAATCACATCGGGAGACAATTTTGGGAGTTCGACCCAAATCTCGGAACACTGGAAGAGCGAGCTCAGGTGGAAAAAGCACGTCTTGAGTTCGTAACAATCGTTTTCGATCTAAACATATTTGCAAAGGAGAAAGAAAGTGATCGAATAGAGCTACCAACTCAGGTGAAAATAAAAAGTGAAGAAGAAATTAATGAAGAAGTAGTAACAACTGCATTGAGGAGGGGGTTGAGATTTTACTCGACTCTTCAGGATGAGGATGGTTTTTGGCCTGGTGACTGTGGCGGTCCTCTGTTTCTTCTACCTGGCTTGAACAAAGATTGTGGTTGGGGACTGCATATAGAAGGGCCAAGTACATGGTTTTGTACAGCTTTGTCCTATGTGAGTTTGAGATTGCTCGGAGAGGAGATGGATGGCGGTGATGGCGGCATGGAGAAGGCAAGAAAATGGATTCTTCACCTGCGTGGAGGAGTTACCACCATCCCATCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATGAATGGGATAGTAACAATCCACTGCCTCCTGAACTGTGGCTTTTACCGTACTTTCTCCCAGTACATCCAGGTCGAATGTGGTGTCACTGTAGGATGGTATACCTGCCCATGTCATATTTATACGGCAAGAGATTTGTTGGTCCCATCAACGGCCTTATTTTATCCCTCAGAACAGAGCTTTATACCTTTCCCTATCACCTGATTGATTGGGATCAAGCAAGAAATCTATGCACCAAGGAGGATTTGTATTACCCACATCCCATTTTACAGGACGTGCTGTGGGGGTGTCTTCATAAGGTTGGGGAACCTCTTCTCATGAGATGGCCATTCTCTAACCTCAGGCAAAAAGCTCTCACCACTGTGATGGACCACGTACACTATGAGGATGAAAACACTCAGTATATCTGCATTGGAGCTGTCAATAAGGTAATAAACATGATTTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCATACAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCCCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTCTCTACCAACCTTGTGAATGAATATGGTTCCGTGCTTAAGAAGGCGCACTATTTCATAAAAAAAACACAAGCTAGAGACAGCTATGGCAATCTGCGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCTAGACAATGGTTGGCCTGCTTCAGATTGGACAGCTGAAGGTTTGAAGCACTCTTATTATCACGTATGCCCTGCAAAATTGTGGGAGAAGATAGCAGCTGATCAACTGCTTGATGCTGTTAATCTAGTATTATCCCTACAGAACAGCAATCGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATACTCAATCCAGCTGAAACATTTGGAGATATCATGATTGATTATCAGTATGTGGAATGCACATCATCAGCAGTTGAAGGCCTTAATGCATTCATGAAGTTATATCCAGGGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGACTGGTGGCAAGACCTATCAGACAAGTAATAGCAGTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTCTCATGCCAAAACCAGGTAAAGGGGCGTAACACAAATCTCGTAGGAAACAAACCACATATAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAGACGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAAAAACTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTCACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

703

Amino Acids

80.42

Weight (kDa)

6.48

Isoelectric Point (pI)

45.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	125 - 367	1.9e-26	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	380 - 702	3e-35	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	528
Acc36I	ACCTGC	2 cut(s)	528, 696
Acc65I	GGTACC	1 cut(s)	1281
AccB1I	GGYRCC	1 cut(s)	1281
AccI	GTMKAC	1 cut(s)	684
AciI	CCGC	5 cut(s)	345, 477, 486, 1302, 2070
AclWI	GGATC	6 cut(s)	804, 1050, 1131, 1144, 1494, 1951
AcoI	YGGCCR	1 cut(s)	908
AcsI	RAATTY	1 cut(s)	1048
AcuI	CTGAAG	4 cut(s)	32, 293, 1326, 1377
AfaI	GTAC	7 cut(s)	415, 427, 573, 635, 648, 960, 1283
AfiI	CCNNNNNNNGG	9 cut(s)	104, 550, 551, 734, 1146, 1147, 1509, 1849, 1855
AflII	CTTAAG	1 cut(s)	1214
AgsI	TTSAA	7 cut(s)	373, 1077, 1169, 1366, 1595, 1931, 2044
AjiI	CACGTC	2 cut(s)	143, 859
AjnI	CCWGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
AleI	CACNNNNGTG	3 cut(s)	56, 522, 944
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	2 cut(s)	70, 1248
AlwI	GGATC	6 cut(s)	804, 1050, 1131, 1144, 1494, 1951
AoxI	GGCC	7 cut(s)	329, 407, 736, 908, 1307, 1334, 1597
ApeKI	GCWGC	2 cut(s)	1415, 1973
ApoI	RAATTY	1 cut(s)	1048
ArsI	GACNNNNNNTTYG	2 cut(s)	652, 684
AseI	ATTAAT	1 cut(s)	252
Asp700I	GAANNNNTTC	1 cut(s)	894
Asp718I	GGTACC	1 cut(s)	1281
AspLEI	GCGC	1 cut(s)	1225
AspS9I	GGNCC	6 cut(s)	347, 407, 725, 952, 1308, 1951
AsuHPI	GGTGA	7 cut(s)	238, 347, 491, 509, 773, 931, 1955
AvaII	GGWCC	4 cut(s)	347, 725, 952, 1951
AxyI	CCTNAGG	1 cut(s)	923
BaeGI	GKGCMC	1 cut(s)	2101
BalI	TGGCCA	1 cut(s)	910
BamHI	GGATCC	1 cut(s)	1136
BanI	GGYRCC	1 cut(s)	1281
BanII	GRGCYC	1 cut(s)	127
BbsI	GAAGAC	2 cut(s)	866, 2032
Bbv12I	GWGCWC	1 cut(s)	127
BbvI	GCAGC	2 cut(s)	1427, 1985
BceAI	ACGGC	2 cut(s)	724, 751
BcgI	CGANNNNNNTGC	2 cut(s)	1945, 1979
BciT130I	CCWGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
BclI	TGATCA	2 cut(s)	1420, 1986
BcoDI	GTCTC	2 cut(s)	70, 1248
BfaI	CTAG	3 cut(s)	1251, 1322, 1445
BfmI	CTRYAG	3 cut(s)	673, 1457, 2074
BfrI	CTTAAG	1 cut(s)	1214
BfuAI	ACCTGC	2 cut(s)	528, 696
BisI	GCNGC	3 cut(s)	487, 1416, 1974
BlsI	GCNGC	3 cut(s)	488, 1417, 1975
BmcAI	AGTACT	1 cut(s)	573
Bme1390I	CCNGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
Bme18I	GGWCC	4 cut(s)	347, 725, 952, 1951
BmgBI	CACGTC	2 cut(s)	143, 859
BmgT120I	GGNCC	6 cut(s)	347, 407, 725, 952, 1308, 1951
BmiI	GGNNCC	7 cut(s)	727, 891, 1138, 1207, 1283, 1952, 1953
BmrFI	CCNGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
BmrI	ACTGGG	1 cut(s)	638
BmsI	GCATC	4 cut(s)	1423, 1490, 1639, 1975
BmuI	ACTGGG	1 cut(s)	638
BpiI	GAAGAC	2 cut(s)	866, 2032
Bpu10I	CCTNAGC	1 cut(s)	126
BpuEI	CTTGAG	1 cut(s)	167
BsaAI	YACGTR	2 cut(s)	958, 1384
BsaBI	GATNNNNATC	2 cut(s)	39, 795
BsaJI	CCNNGG	3 cut(s)	44, 819, 1625
BsaXI	ACNNNNNCTCC	2 cut(s)	815, 845
Bsc4I	CCNNNNNNNGG	9 cut(s)	104, 550, 551, 734, 1146, 1147, 1509, 1849, 1855
Bse1I	ACTGG	5 cut(s)	117, 644, 1283, 1741, 1909
Bse21I	CCTNAGG	1 cut(s)	923
Bse8I	GATNNNNATC	2 cut(s)	39, 795
BseBI	CCWGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
BseDI	CCNNGG	3 cut(s)	44, 819, 1625
BseJI	GATNNNNATC	2 cut(s)	39, 795
BseLI	CCNNNNNNNGG	9 cut(s)	104, 550, 551, 734, 1146, 1147, 1509, 1849, 1855
BseMII	CTCAG	6 cut(s)	140, 236, 582, 766, 937, 995
BseNI	ACTGG	5 cut(s)	117, 644, 1283, 1741, 1909
BseRI	GAGGAG	3 cut(s)	295, 479, 543
BseSI	GKGCMC	1 cut(s)	2101
BseXI	GCAGC	2 cut(s)	1427, 1985
BseYI	CCCAGC	2 cut(s)	1146, 1818
BshFI	GGCC	7 cut(s)	331, 409, 738, 910, 1309, 1336, 1599
BshNI	GGYRCC	1 cut(s)	1281
BsiHKAI	GWGCWC	1 cut(s)	127
BslFI	GGGAC	3 cut(s)	405, 711, 1964
BslI	CCNNNNNNNGG	9 cut(s)	104, 550, 551, 734, 1146, 1147, 1509, 1849, 1855
BsmAI	GTCTC	2 cut(s)	70, 1248
BsmFI	GGGAC	3 cut(s)	405, 711, 1964
BsmI	GAATGC	2 cut(s)	1580, 1607
BsnI	GGCC	7 cut(s)	331, 409, 738, 910, 1309, 1336, 1599
Bsp1286I	GDGCHC	2 cut(s)	127, 2101
Bsp1407I	TGTACA	1 cut(s)	425
Bsp143I	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
Bsp19I	CCATGG	1 cut(s)	44
BspACI	CCGC	5 cut(s)	345, 477, 486, 1302, 2070
BspANI	GGCC	7 cut(s)	331, 409, 738, 910, 1309, 1336, 1599
BspCNI	CTCAG	6 cut(s)	139, 235, 581, 765, 936, 994
BspHI	TCATGA	3 cut(s)	900, 1551, 1611
BspLI	GGNNCC	7 cut(s)	727, 891, 1138, 1207, 1283, 1952, 1953
BspMI	ACCTGC	2 cut(s)	528, 696
BspPI	GGATC	6 cut(s)	804, 1050, 1131, 1144, 1494, 1951
BspQI	GCTCTTC	2 cut(s)	111, 1768
BspT107I	GGYRCC	1 cut(s)	1281
BspTI	CTTAAG	1 cut(s)	1214
BsrGI	TGTACA	1 cut(s)	425
BsrI	ACTGG	5 cut(s)	117, 644, 1283, 1741, 1909
BssECI	CCNNGG	3 cut(s)	44, 819, 1625
BssMI	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
BssNAI	GTATAC	1 cut(s)	685
BssT1I	CCWWGG	2 cut(s)	44, 819
Bst1107I	GTATAC	1 cut(s)	685
Bst2UI	CCWGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
Bst4CI	ACNGT	6 cut(s)	341, 621, 633, 674, 946, 1286
Bst6I	CTCTTC	4 cut(s)	111, 312, 900, 1768
BstAFI	CTTAAG	1 cut(s)	1214
BstAPI	GCANNNNNTGC	1 cut(s)	1658
BstAUI	TGTACA	1 cut(s)	425
BstBAI	YACGTR	2 cut(s)	958, 1384
BstC8I	GCNNGC	2 cut(s)	123, 1338
BstDEI	CTNAG	8 cut(s)	126, 222, 568, 752, 923, 981, 1296, 1797
BstDSI	CCRYGG	1 cut(s)	44
BstHHI	GCGC	1 cut(s)	1225
BstKTI	GATC	9 cut(s)	172, 205, 799, 1045, 1139, 1423, 1502, 1959, 1989
BstMAI	GTCTC	2 cut(s)	70, 1248
BstMBI	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
BstMWI	GCNNNNNNNGC	5 cut(s)	483, 1169, 1658, 1798, 1919
BstNI	CCWGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
BstNSI	RCATGY	2 cut(s)	1656, 1782
BstSCI	CCNGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
BstSFI	CTRYAG	3 cut(s)	673, 1457, 2074
BstSLI	GKGCMC	1 cut(s)	2101
BstV1I	GCAGC	2 cut(s)	1427, 1985
BstV2I	GAAGAC	2 cut(s)	866, 2032
BstX2I	RGATCY	2 cut(s)	1136, 1499
BstXI	CCANNNNNNTGG	2 cut(s)	417, 949
BstYI	RGATCY	2 cut(s)	1136, 1499
BstZ17I	GTATAC	1 cut(s)	685
Bsu36I	CCTNAGG	1 cut(s)	923
BsuRI	GGCC	7 cut(s)	331, 409, 738, 910, 1309, 1336, 1599
BtgI	CCRYGG	1 cut(s)	44
BtrI	CACGTC	2 cut(s)	143, 859
BtsI	GCAGTG	1 cut(s)	605
BtsIMutI	CAGTG	5 cut(s)	110, 605, 670, 942, 1902
BveI	ACCTGC	2 cut(s)	528, 696
Cac8I	GCNNGC	2 cut(s)	123, 1338
CciI	TCATGA	3 cut(s)	900, 1551, 1611
CfoI	GCGC	1 cut(s)	1225
Cfr13I	GGNCC	6 cut(s)	347, 407, 725, 952, 1308, 1951
CsiI	ACCWGGT	1 cut(s)	1848
Csp6I	GTAC	7 cut(s)	414, 426, 572, 634, 647, 959, 1282
CspCI	CAANNNNNGTGG	2 cut(s)	364, 399
CviQI	GTAC	7 cut(s)	414, 426, 572, 634, 647, 959, 1282
DdeI	CTNAG	8 cut(s)	126, 222, 568, 752, 923, 981, 1296, 1797
DpnI	GATC	9 cut(s)	171, 204, 798, 1044, 1138, 1422, 1501, 1958, 1988
DpnII	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
EaeI	YGGCCR	1 cut(s)	908
Eam1104I	CTCTTC	4 cut(s)	111, 312, 900, 1768
EarI	CTCTTC	4 cut(s)	111, 312, 900, 1768
EciI	GGCGGA	2 cut(s)	1317, 2059
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	2 cut(s)	44, 819
Eco147I	AGGCCT	1 cut(s)	1599
Eco24I	GRGCYC	1 cut(s)	127
Eco32I	GATATC	1 cut(s)	1549
Eco47I	GGWCC	4 cut(s)	347, 725, 952, 1951
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	4 cut(s)	32, 293, 1326, 1377
Eco81I	CCTNAGG	1 cut(s)	923
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	1 cut(s)	1951
EcoRI	GAATTC	1 cut(s)	1048
EcoRII	CCWGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
EcoRV	GATATC	1 cut(s)	1549
EcoT14I	CCWWGG	2 cut(s)	44, 819
EcoT22I	ATGCAT	1 cut(s)	1609
EcoT38I	GRGCYC	1 cut(s)	127
ErhI	CCWWGG	2 cut(s)	44, 819
FalI	AAGNNNNNCTT	2 cut(s)	129, 161
FaqI	GGGAC	3 cut(s)	405, 711, 1964
FauNDI	CATATG	1 cut(s)	1486
FbaI	TGATCA	2 cut(s)	1420, 1986
FblI	GTMKAC	1 cut(s)	684
Fnu4HI	GCNGC	3 cut(s)	487, 1416, 1974
FriOI	GRGCYC	1 cut(s)	127
Fsp4HI	GCNGC	3 cut(s)	487, 1416, 1974
FspBI	CTAG	3 cut(s)	1251, 1322, 1445
GlaI	GCGC	1 cut(s)	1224
GluI	GCNGC	3 cut(s)	487, 1416, 1974
GsaI	CCCAGC	2 cut(s)	1150, 1822
HaeIII	GGCC	7 cut(s)	331, 409, 738, 910, 1309, 1336, 1599
HhaI	GCGC	1 cut(s)	1225
Hin6I	GCGC	1 cut(s)	1223
HinP1I	GCGC	1 cut(s)	1223
HinfI	GANTC	5 cut(s)	304, 511, 1080, 1808, 2037
HphI	GGTGA	7 cut(s)	238, 347, 491, 509, 773, 931, 1955
Hpy166II	GTNNAC	3 cut(s)	685, 961, 1900
Hpy188I	TCNGA	9 cut(s)	22, 107, 461, 755, 1047, 1054, 1345, 1635, 1755
Hpy188III	TCNNGA	9 cut(s)	73, 146, 311, 614, 901, 1077, 1322, 1552, 1612
Hpy8I	GTNNAC	3 cut(s)	685, 961, 1900
HpyAV	CCTTC	8 cut(s)	7, 17, 398, 490, 1213, 1321, 1352, 1589
HpyCH4III	ACNGT	6 cut(s)	341, 621, 633, 674, 946, 1286
HpyCH4IV	ACGT	4 cut(s)	142, 858, 957, 1383
HpyF10VI	GCNNNNNNNGC	5 cut(s)	483, 1169, 1658, 1798, 1919
HpyF3I	CTNAG	8 cut(s)	126, 222, 568, 752, 923, 981, 1296, 1797
HpySE526I	ACGT	4 cut(s)	142, 858, 957, 1383
HspAI	GCGC	1 cut(s)	1223
KflI	GGGWCCC	1 cut(s)	1951
KpnI	GGTACC	1 cut(s)	1285
Ksp22I	TGATCA	2 cut(s)	1420, 1986
Kzo9I	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
LguI	GCTCTTC	2 cut(s)	111, 1768
LmnI	GCTCC	1 cut(s)	998
Lsp1109I	GCAGC	2 cut(s)	1427, 1985
LweI	GCATC	4 cut(s)	1423, 1490, 1639, 1975
MabI	ACCWGGT	1 cut(s)	1848
MaeI	CTAG	3 cut(s)	1251, 1322, 1445
MaeII	ACGT	4 cut(s)	142, 858, 957, 1383
MalI	GATC	9 cut(s)	171, 204, 798, 1044, 1138, 1422, 1501, 1958, 1988
MboI	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
MflI	RGATCY	2 cut(s)	1136, 1499
MhlI	GDGCHC	2 cut(s)	127, 2101
MlsI	TGGCCA	1 cut(s)	910
MluCI	AATT	6 cut(s)	80, 249, 1048, 1397, 1926, 1990
MluNI	TGGCCA	1 cut(s)	910
MlyI	GAGTC	2 cut(s)	298, 2046
MmeI	TCCRAC	1 cut(s)	1494
Mox20I	TGGCCA	1 cut(s)	910
Mph1103I	ATGCAT	1 cut(s)	1609
MroXI	GAANNNNTTC	1 cut(s)	894
MscI	TGGCCA	1 cut(s)	910
MseI	TTAA	5 cut(s)	252, 1215, 1440, 1602, 1730
MslI	CAYNNNNRTG	5 cut(s)	56, 522, 944, 947, 1063
Msp20I	TGGCCA	1 cut(s)	910
MspA1I	CMGCKG	3 cut(s)	1355, 1418, 1532
MspCI	CTTAAG	1 cut(s)	1214
MspR9I	CCNGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
Mva1269I	GAATGC	2 cut(s)	1580, 1607
MvaI	CCWGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
MwoI	GCNNNNNNNGC	5 cut(s)	483, 1169, 1658, 1798, 1919
NcoI	CCATGG	1 cut(s)	44
NdeI	CATATG	1 cut(s)	1486
NdeII	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
NlaIV	GGNNCC	7 cut(s)	727, 891, 1138, 1207, 1283, 1952, 1953
NmuCI	GTSAC	3 cut(s)	335, 668, 1286
NsiI	ATGCAT	1 cut(s)	1609
NspI	RCATGY	2 cut(s)	1656, 1782
OliI	CACNNNNGTG	3 cut(s)	56, 522, 944
PagI	TCATGA	3 cut(s)	900, 1551, 1611
PaqCI	CACCTGC	1 cut(s)	528
PceI	AGGCCT	1 cut(s)	1599
PciSI	GCTCTTC	2 cut(s)	111, 1768
PcsI	WCGNNNNNNNCGW	1 cut(s)	159
PctI	GAATGC	2 cut(s)	1580, 1607
PdmI	GAANNNNTTC	1 cut(s)	894
PfeI	GAWTC	3 cut(s)	511, 1080, 1808
PkrI	GCNGC	3 cut(s)	488, 1417, 1975
PleI	GAGTC	2 cut(s)	298, 2045
PpsI	GAGTC	2 cut(s)	298, 2045
Ppu21I	YACGTR	2 cut(s)	958, 1384
PpuMI	RGGWCCY	1 cut(s)	1951
PshBI	ATTAAT	1 cut(s)	252
Psp124BI	GAGCTC	1 cut(s)	127
Psp5II	RGGWCCY	1 cut(s)	1951
Psp6I	CCWGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
PspFI	CCCAGC	2 cut(s)	1146, 1818
PspGI	CCWGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
PspN4I	GGNNCC	7 cut(s)	727, 891, 1138, 1207, 1283, 1952, 1953
PspPI	GGNCC	6 cut(s)	347, 407, 725, 952, 1308, 1951
PspPPI	RGGWCCY	1 cut(s)	1951
PsuI	RGATCY	2 cut(s)	1136, 1499
PvuII	CAGCTG	3 cut(s)	1355, 1418, 1532
RsaI	GTAC	7 cut(s)	415, 427, 573, 635, 648, 960, 1283
RsaNI	GTAC	7 cut(s)	414, 426, 572, 634, 647, 959, 1282
RseI	CAYNNNNRTG	5 cut(s)	56, 522, 944, 947, 1063
SacI	GAGCTC	1 cut(s)	127
SapI	GCTCTTC	2 cut(s)	111, 1768
SaqAI	TTAA	5 cut(s)	252, 1215, 1440, 1602, 1730
SatI	GCNGC	3 cut(s)	487, 1416, 1974
Sau3AI	GATC	9 cut(s)	169, 202, 796, 1042, 1136, 1420, 1499, 1956, 1986
Sau96I	GGNCC	6 cut(s)	347, 407, 725, 952, 1308, 1951
ScaI	AGTACT	1 cut(s)	573
SchI	GAGTC	2 cut(s)	298, 2046
ScrFI	CCNGG	6 cut(s)	333, 366, 654, 1510, 1626, 1850
SduI	GDGCHC	2 cut(s)	127, 2101
SexAI	ACCWGGT	1 cut(s)	1848
SfaNI	GCATC	4 cut(s)	1423, 1490, 1639, 1975
SfcI	CTRYAG	3 cut(s)	673, 1457, 2074
SinI	GGWCC	4 cut(s)	347, 725, 952, 1951
SmiMI	CAYNNNNRTG	5 cut(s)	56, 522, 944, 947, 1063
SmlI	CTYRAG	2 cut(s)	146, 1214
SmoI	CTYRAG	2 cut(s)	146, 1214
Sse9I	AATT	6 cut(s)	80, 249, 1048, 1397, 1926, 1990
SseBI	AGGCCT	1 cut(s)	1599
SsiI	CCGC	5 cut(s)	345, 477, 486, 1302, 2070
SspMI	CTAG	3 cut(s)	1251, 1322, 1445
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	1 cut(s)	1599
StyD4I	CCNGG	6 cut(s)	331, 364, 652, 1508, 1624, 1848
StyI	CCWWGG	2 cut(s)	44, 819
TaaI	ACNGT	6 cut(s)	341, 621, 633, 674, 946, 1286
TaiI	ACGT	4 cut(s)	145, 861, 960, 1386
TaqI	TCGA	8 cut(s)	93, 168, 205, 302, 658, 1471, 1806, 1969
TasI	AATT	6 cut(s)	80, 249, 1048, 1397, 1926, 1990
TatI	WGTACW	4 cut(s)	413, 425, 571, 646
TauI	GCSGC	1 cut(s)	489
TfiI	GAWTC	3 cut(s)	511, 1080, 1808
Tru1I	TTAA	5 cut(s)	252, 1215, 1440, 1602, 1730
Tru9I	TTAA	5 cut(s)	252, 1215, 1440, 1602, 1730
TscAI	CASTG	5 cut(s)	117, 612, 677, 949, 1909
TseFI	GTSAC	3 cut(s)	335, 668, 1286
TseI	GCWGC	2 cut(s)	1415, 1973
Tsp45I	GTSAC	3 cut(s)	335, 668, 1286
TspGWI	ACGGA	2 cut(s)	1198, 2015
TspRI	CASTG	5 cut(s)	117, 612, 677, 949, 1909
Vha464I	CTTAAG	1 cut(s)	1214
VpaK11BI	GGWCC	4 cut(s)	347, 725, 952, 1951
VspI	ATTAAT	1 cut(s)	252
XapI	RAATTY	1 cut(s)	1048
XbaI	TCTAGA	1 cut(s)	1321
XceI	RCATGY	2 cut(s)	1656, 1782
XcmI	CCANNNNNNNNNTGG	1 cut(s)	660
XmiI	GTMKAC	1 cut(s)	684
XmnI	GAANNNNTTC	1 cut(s)	894
XspI	CTAG	3 cut(s)	1251, 1322, 1445
ZrmI	AGTACT	1 cut(s)	573
Zsp2I	ATGCAT	1 cut(s)	1609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.