Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

13626046 .. 13644654

Gene Structure 18609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ36959

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAAGAGATGGTAACCCATGGCTCACAAGTGTGAATAATCACATCGGGAGACAATTCTGGGAGTTCGACCCAAATCTCGGAACATCGGAAGAGCGAGCTCAGGTGGAAAAAGTACGTCTTGAGTTTCGTAACAATCGTTTTCGAACCGAACATAGTTCTGATCTATTAATGAGACTTCAATTTGCAAAGGAGAAAGAAAGTGATCCAATGAAGCTACCAGTTCAGGTGAAAATAAAAAGTGAAGAAGAAATTAATGAAGAAGTAGTAACAGCCACATTGAGGAGGGGGTTGAGATTTTACTCGACTCTTCAGGCTGAGGATGGTTTTTGGCCTGGTGACTATGGTGGTCCTCTGTTTCTTCTACTTGGCTTGGTTATTGGATTATCTGTAACAGGGGCCCTTGATGCAATCTTGCCACACGAACATTCAAAGGAGATCCGCCGCTATCTTTATAACCATCAGAACAAAGATGGAGGTTGGGGACTGCATATAGAAGGGCGAAGCACAATGTTTTGTACAGCTCTGTCCTATGTGAGTTTGAGATTGCTCGGAGAGGAGATGGACGGCGGTGATGGCGCCATGGAGAAGGGAAGAAAATGGATTCTTCACCGTGGAGGAGTTACTAACATCCCATCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATGAATGGGACGGTAACAATCCACTGCCTCCTGAATTGTGGCTTCTACCGTACTTTCTCCCAGTACACCCAGGTCGAATGTGGTGTCACTGTAGGATGGTGTACCTGCCTATGTCATATTTATACGGCAACAGATTTGTTGGTCCCATCAACGGCCTTGATTTATCCCTCAGAAGAGAGCTTTATACCCTTCCCTATCACCTGATTGATTGGGATGAAGCAAGAAATCTATGCGCCAAGGAGGATTTGTATTACCCACATCCCATTTTACAGGACGTGTTGTGGGGCTGTCTTCATAAGGTTGGGGAACCTCTTCTCATGAGATGGCCATTGTCTAACCTCAGGCAAAAAGCTCTCACCACTGTAATGGACCACATACACTATGAGGATGAAAACACTCAGTATATCTGCATTGGACCTGTCAATAAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCCGAATTCAGAAGCATACAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCTCAGTGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTTTCTACCAACCTTGTGAATGAATATGGTTCTGTGCTTAAGAAGGCGCACTATTTCATCAAAAATACGCAAGTTAGGAGAGACAGCTATGGCAATCTTAGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCCAGACAATGGTTGGCCTGTCTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTATTATCACGTATGCCCTGCGAAATTGTGGGAGACGCCATAGCAGCTGATCAACTGCATGATGCTGTTAATGTAGTACTATCCTTACAGGTACAACTTGACCTTGTGACATCTTACAGTACAATCGCAAAATATTTATGTAATCACAAGAGCAATGACATGTATTTTATTTCTTTGACTCAACAGAACAGCAATGGAGGGTTTGCTTCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCATTATTGATTATCAGTACGTGGAATGCACATCAGCAGCAGTTGAAGGCCTTAATGCATTCATGAAATTATATCCAGGGCATCGGAATAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATACAACTGCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTAGTGGCAAGACCTATCAGACAAGTATTAGCATTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTTTCATGCCAAAACAAGGGTGGAAGGAATCCTATCCCCTTGCATCAAGCAGCAAAGGTATTGATTAATTCACAGATGGAAAATGGAGATTTCCCACAACAGGTATTTGTTATTGTAAATACTGTACAAGGTTTCAGAGGCATAACATTACAATTTACAACCTTGGACTTGCTATGTTTATTTCAACAGCAAATCATGGGAGTCTTCAACAAAAATTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTGGAGTTGGAGAGTATCGAAACCGGGTACTGGTACTTCACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

799

Amino Acids

91.21

Weight (kDa)

6.46

Isoelectric Point (pI)

43.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	146 - 405	2.1e-35	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	557 - 786	3.2e-27	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	480
Acc36I	ACCTGC	1 cut(s)	810
Acc65I	GGTACC	1 cut(s)	1398
AccB1I	GGYRCC	2 cut(s)	602, 1398
AccB7I	CCANNNNNTGG	1 cut(s)	1445
AciI	CCGC	5 cut(s)	466, 469, 594, 1419, 2322
AclWI	GGATC	6 cut(s)	224, 457, 1151, 1164, 1258, 1713
AcoI	YGGCCR	1 cut(s)	1022
AcsI	RAATTY	1 cut(s)	1162
AcuI	CTGAAG	3 cut(s)	32, 320, 1494
AcyI	GRCGYC	2 cut(s)	603, 1530
AflII	CTTAAG	1 cut(s)	1328
AflIII	ACRYGT	2 cut(s)	972, 1653
AgsI	TTSAA	8 cut(s)	206, 456, 1191, 1283, 1483, 1814, 2273, 2296
AjiI	CACGTC	1 cut(s)	973
AjnI	CCWGG	4 cut(s)	358, 766, 1727, 1843
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	5 cut(s)	70, 193, 1365, 1462, 1521
AlwI	GGATC	6 cut(s)	224, 457, 1151, 1164, 1258, 1713
AoxI	GGCC	7 cut(s)	356, 423, 850, 1022, 1424, 1451, 1816
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	4 cut(s)	1487, 1538, 1805, 2138
ApoI	RAATTY	1 cut(s)	1162
ArsI	GACNNNNNNTTYG	2 cut(s)	766, 798
AseI	ATTAAT	3 cut(s)	194, 279, 2154
Asp700I	GAANNNNTTC	1 cut(s)	1008
Asp718I	GGTACC	1 cut(s)	1398
AspLEI	GCGC	3 cut(s)	605, 932, 1339
AspS9I	GGNCC	7 cut(s)	374, 423, 424, 839, 1066, 1112, 1425
AsuC2I	CCSGG	1 cut(s)	2378
AsuHPI	GGTGA	6 cut(s)	265, 374, 608, 626, 887, 1045
AsuII	TTCGAA	1 cut(s)	169
AvaII	GGWCC	4 cut(s)	374, 839, 1066, 1112
AxyI	CCTNAGG	1 cut(s)	1037
BaeGI	GKGCMC	2 cut(s)	427, 2353
BalI	TGGCCA	1 cut(s)	1024
BamHI	GGATCC	1 cut(s)	1156
BanI	GGYRCC	2 cut(s)	602, 1398
BanII	GRGCYC	2 cut(s)	127, 427
BarI	GAAGNNNNNNTAC	1 cut(s)	2373
BbsI	GAAGAC	2 cut(s)	980, 2284
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	342
BbvI	GCAGC	4 cut(s)	1499, 1550, 1817, 2150
BceAI	ACGGC	3 cut(s)	607, 838, 865
BciT130I	CCWGG	4 cut(s)	360, 768, 1729, 1845
BclI	TGATCA	2 cut(s)	1543, 1740
BcnI	CCSGG	1 cut(s)	2378
BcoDI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 1521
BfaI	CTAG	1 cut(s)	1992
BfmI	CTRYAG	2 cut(s)	787, 2326
BfoI	RGCGCY	1 cut(s)	606
BfrI	CTTAAG	1 cut(s)	1328
BfuAI	ACCTGC	1 cut(s)	810
BisI	GCNGC	5 cut(s)	469, 1488, 1539, 1806, 2139
BlsI	GCNGC	5 cut(s)	470, 1489, 1540, 1807, 2140
BmcAI	AGTACT	2 cut(s)	687, 1572
Bme1390I	CCNGG	5 cut(s)	360, 768, 1729, 1845, 2378
Bme18I	GGWCC	4 cut(s)	374, 839, 1066, 1112
BmgBI	CACGTC	1 cut(s)	973
BmgT120I	GGNCC	7 cut(s)	374, 423, 424, 839, 1066, 1112, 1425
BmiI	GGNNCC	7 cut(s)	424, 425, 604, 841, 1005, 1158, 1400
BmrFI	CCNGG	5 cut(s)	360, 768, 1729, 1845, 2378
BmrI	ACTGGG	1 cut(s)	752
BmsI	GCATC	4 cut(s)	421, 1546, 1858, 2140
BmuI	ACTGGG	1 cut(s)	752
BpiI	GAAGAC	2 cut(s)	980, 2284
BpmI	CTGGAG	1 cut(s)	1422
Bpu10I	CCTNAGC	2 cut(s)	126, 342
Bpu14I	TTCGAA	1 cut(s)	169
BpuEI	CTTGAG	1 cut(s)	167
BpuMI	CCSGG	1 cut(s)	2378
BsaAI	YACGTR	2 cut(s)	1505, 1789
BsaHI	GRCGYC	2 cut(s)	603, 1530
BsaJI	CCNNGG	7 cut(s)	44, 606, 637, 766, 933, 1844, 2250
BsaXI	ACNNNNNCTCC	2 cut(s)	929, 959
Bse1I	ACTGG	4 cut(s)	245, 758, 1400, 2388
Bse21I	CCTNAGG	1 cut(s)	1037
Bse3DI	GCAATG	2 cut(s)	1654, 1693
BseBI	CCWGG	4 cut(s)	360, 768, 1729, 1845
BseDI	CCNNGG	7 cut(s)	44, 606, 637, 766, 933, 1844, 2250
BseGI	GGATG	8 cut(s)	352, 654, 798, 916, 955, 1090, 1270, 1427
BseMI	GCAATG	2 cut(s)	1654, 1693
BseMII	CTCAG	8 cut(s)	140, 333, 696, 880, 1051, 1109, 1268, 1473
BseNI	ACTGG	4 cut(s)	245, 758, 1400, 2388
BseRI	GAGGAG	3 cut(s)	322, 596, 657
BseSI	GKGCMC	2 cut(s)	427, 2353
BseXI	GCAGC	4 cut(s)	1499, 1550, 1817, 2150
BseYI	CCCAGC	2 cut(s)	1260, 2073
BshFI	GGCC	7 cut(s)	358, 425, 852, 1024, 1426, 1453, 1818
BshNI	GGYRCC	2 cut(s)	602, 1398
BsiHKAI	GWGCWC	1 cut(s)	127
BsiSI	CCGG	1 cut(s)	2377
BslFI	GGGAC	3 cut(s)	522, 719, 825
BsmAI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 1521
BsmBI	CGTCTC	1 cut(s)	1521
BsmFI	GGGAC	3 cut(s)	522, 719, 825
BsmI	GAATGC	2 cut(s)	1799, 1826
BsnI	GGCC	7 cut(s)	358, 425, 852, 1024, 1426, 1453, 1818
Bsp119I	TTCGAA	1 cut(s)	169
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	3 cut(s)	127, 427, 2353
Bsp1407I	TGTACA	3 cut(s)	542, 1466, 2212
Bsp143I	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
Bsp19I	CCATGG	2 cut(s)	44, 606
BspACI	CCGC	5 cut(s)	466, 469, 594, 1419, 2322
BspANI	GGCC	7 cut(s)	358, 425, 852, 1024, 1426, 1453, 1818
BspCNI	CTCAG	8 cut(s)	139, 334, 695, 879, 1050, 1108, 1267, 1472
BspHI	TCATGA	2 cut(s)	1014, 1830
BspLI	GGNNCC	7 cut(s)	424, 425, 604, 841, 1005, 1158, 1400
BspMI	ACCTGC	1 cut(s)	810
BspPI	GGATC	6 cut(s)	224, 457, 1151, 1164, 1258, 1713
BspQI	GCTCTTC	2 cut(s)	111, 2023
BspT104I	TTCGAA	1 cut(s)	169
BspT107I	GGYRCC	2 cut(s)	602, 1398
BspTI	CTTAAG	1 cut(s)	1328
BsrDI	GCAATG	2 cut(s)	1654, 1693
BsrGI	TGTACA	3 cut(s)	542, 1466, 2212
BsrI	ACTGG	4 cut(s)	245, 758, 1400, 2388
BssECI	CCNNGG	7 cut(s)	44, 606, 637, 766, 933, 1844, 2250
BssMI	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
BssNI	GRCGYC	2 cut(s)	603, 1530
BssT1I	CCWWGG	4 cut(s)	44, 606, 933, 2250
Bst2UI	CCWGG	4 cut(s)	360, 768, 1729, 1845
Bst4CI	ACNGT	8 cut(s)	638, 710, 747, 788, 1060, 1403, 1613, 2212
Bst6I	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 2023
BstACI	GRCGYC	2 cut(s)	603, 1530
BstAFI	CTTAAG	1 cut(s)	1328
BstAPI	GCANNNNNTGC	1 cut(s)	1877
BstAUI	TGTACA	3 cut(s)	542, 1466, 2212
BstBAI	YACGTR	2 cut(s)	1505, 1789
BstBI	TTCGAA	1 cut(s)	169
BstC8I	GCNNGC	1 cut(s)	123
BstDSI	CCRYGG	3 cut(s)	44, 606, 637
BstEII	GGTNACC	1 cut(s)	38
BstF5I	GGATG	8 cut(s)	352, 654, 798, 916, 955, 1090, 1270, 1427
BstH2I	RGCGCY	1 cut(s)	606
BstHHI	GCGC	3 cut(s)	605, 932, 1339
BstKTI	GATC	8 cut(s)	190, 232, 465, 1159, 1253, 1546, 1721, 1743
BstMAI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 1521
BstMBI	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
BstMWI	GCNNNNNNNGC	6 cut(s)	431, 600, 1283, 1538, 1877, 2053
BstNI	CCWGG	4 cut(s)	360, 768, 1729, 1845
BstNSI	RCATGY	3 cut(s)	1657, 1875, 2037
BstPI	GGTNACC	1 cut(s)	38
BstSCI	CCNGG	5 cut(s)	358, 766, 1727, 1843, 2376
BstSFI	CTRYAG	2 cut(s)	787, 2326
BstSLI	GKGCMC	2 cut(s)	427, 2353
BstV1I	GCAGC	4 cut(s)	1499, 1550, 1817, 2150
BstV2I	GAAGAC	2 cut(s)	980, 2284
BstX2I	RGATCY	4 cut(s)	462, 1156, 1250, 1718
BstXI	CCANNNNNNTGG	1 cut(s)	1063
BstYI	RGATCY	4 cut(s)	462, 1156, 1250, 1718
Bsu36I	CCTNAGG	1 cut(s)	1037
BsuRI	GGCC	7 cut(s)	358, 425, 852, 1024, 1426, 1453, 1818
BtgI	CCRYGG	3 cut(s)	44, 606, 637
BtrI	CACGTC	1 cut(s)	973
BtsCI	GGATG	8 cut(s)	352, 654, 798, 916, 955, 1090, 1270, 1427
BtsI	GCAGTG	1 cut(s)	719
BtsIMutI	CAGTG	4 cut(s)	719, 784, 1056, 1262
BveI	ACCTGC	1 cut(s)	810
Cac8I	GCNNGC	1 cut(s)	123
CciI	TCATGA	2 cut(s)	1014, 1830
CfoI	GCGC	3 cut(s)	605, 932, 1339
Cfr13I	GGNCC	7 cut(s)	374, 423, 424, 839, 1066, 1112, 1425
CseI	GACGC	1 cut(s)	1538
DinI	GGCGCC	1 cut(s)	604
DpnI	GATC	8 cut(s)	189, 231, 464, 1158, 1252, 1545, 1720, 1742
DpnII	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
EaeI	YGGCCR	1 cut(s)	1022
Eam1104I	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 2023
EarI	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 2023
EciI	GGCGGA	3 cut(s)	455, 1434, 2311
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	4 cut(s)	44, 606, 933, 2250
Eco147I	AGGCCT	1 cut(s)	1818
Eco24I	GRGCYC	2 cut(s)	127, 427
Eco32I	GATATC	1 cut(s)	1768
Eco47I	GGWCC	4 cut(s)	374, 839, 1066, 1112
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	3 cut(s)	32, 320, 1494
Eco81I	CCTNAGG	1 cut(s)	1037
Eco91I	GGTNACC	1 cut(s)	38
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	2 cut(s)	423, 424
EcoO65I	GGTNACC	1 cut(s)	38
EcoRI	GAATTC	1 cut(s)	1162
EcoRII	CCWGG	4 cut(s)	358, 766, 1727, 1843
EcoRV	GATATC	1 cut(s)	1768
EcoT14I	CCWWGG	4 cut(s)	44, 606, 933, 2250
EcoT22I	ATGCAT	1 cut(s)	1828
EcoT38I	GRGCYC	2 cut(s)	127, 427
EgeI	GGCGCC	1 cut(s)	604
EheI	GGCGCC	1 cut(s)	604
ErhI	CCWWGG	4 cut(s)	44, 606, 933, 2250
Esp3I	CGTCTC	1 cut(s)	1521
FalI	AAGNNNNNCTT	4 cut(s)	129, 161, 1275, 1307
FaqI	GGGAC	3 cut(s)	522, 719, 825
FauNDI	CATATG	1 cut(s)	1705
FbaI	TGATCA	2 cut(s)	1543, 1740
Fnu4HI	GCNGC	5 cut(s)	469, 1488, 1539, 1806, 2139
FokI	GGATG	8 cut(s)	359, 641, 805, 923, 942, 1097, 1277, 1434
FriOI	GRGCYC	2 cut(s)	127, 427
Fsp4HI	GCNGC	5 cut(s)	469, 1488, 1539, 1806, 2139
FspBI	CTAG	1 cut(s)	1992
GlaI	GCGC	3 cut(s)	604, 931, 1338
GluI	GCNGC	5 cut(s)	469, 1488, 1539, 1806, 2139
GsaI	CCCAGC	2 cut(s)	1264, 2077
GsuI	CTGGAG	1 cut(s)	1422
HaeII	RGCGCY	1 cut(s)	606
HaeIII	GGCC	7 cut(s)	358, 425, 852, 1024, 1426, 1453, 1818
HapII	CCGG	1 cut(s)	2377
HgaI	GACGC	1 cut(s)	1538
HhaI	GCGC	3 cut(s)	605, 932, 1339
Hin1I	GRCGYC	2 cut(s)	603, 1530
Hin6I	GCGC	3 cut(s)	603, 930, 1337
HinP1I	GCGC	3 cut(s)	603, 930, 1337
HinfI	GANTC	7 cut(s)	331, 628, 1194, 1672, 2063, 2116, 2289
HpaII	CCGG	1 cut(s)	2377
HphI	GGTGA	6 cut(s)	265, 374, 608, 626, 887, 1045
Hpy166II	GTNNAC	2 cut(s)	763, 799
Hpy188III	TCNNGA	8 cut(s)	73, 146, 728, 1015, 1191, 1439, 1831, 1900
Hpy8I	GTNNAC	2 cut(s)	763, 799
HpyCH4III	ACNGT	8 cut(s)	638, 710, 747, 788, 1060, 1403, 1613, 2212
HpyCH4IV	ACGT	4 cut(s)	142, 972, 1504, 1788
HpyF10VI	GCNNNNNNNGC	6 cut(s)	431, 600, 1283, 1538, 1877, 2053
HpySE526I	ACGT	4 cut(s)	142, 972, 1504, 1788
Hsp92I	GRCGYC	2 cut(s)	603, 1530
HspAI	GCGC	3 cut(s)	603, 930, 1337
KasI	GGCGCC	1 cut(s)	602
KpnI	GGTACC	1 cut(s)	1402
Ksp22I	TGATCA	2 cut(s)	1543, 1740
Kzo9I	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
LguI	GCTCTTC	2 cut(s)	111, 2023
Lsp1109I	GCAGC	4 cut(s)	1499, 1550, 1817, 2150
LweI	GCATC	4 cut(s)	421, 1546, 1858, 2140
MaeI	CTAG	1 cut(s)	1992
MaeII	ACGT	4 cut(s)	142, 972, 1504, 1788
MalI	GATC	8 cut(s)	189, 231, 464, 1158, 1252, 1545, 1720, 1742
MboI	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
MflI	RGATCY	4 cut(s)	462, 1156, 1250, 1718
MhlI	GDGCHC	3 cut(s)	127, 427, 2353
MlsI	TGGCCA	1 cut(s)	1024
MluNI	TGGCCA	1 cut(s)	1024
Mly113I	GGCGCC	1 cut(s)	603
MlyI	GAGTC	3 cut(s)	325, 1666, 2298
MmeI	TCCRAC	2 cut(s)	1713, 2341
Mox20I	TGGCCA	1 cut(s)	1024
Mph1103I	ATGCAT	1 cut(s)	1828
MroXI	GAANNNNTTC	1 cut(s)	1008
MscI	TGGCCA	1 cut(s)	1024
MseI	TTAA	7 cut(s)	194, 279, 1329, 1563, 1821, 1985, 2154
MslI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
Msp20I	TGGCCA	1 cut(s)	1024
MspA1I	CMGCKG	3 cut(s)	1472, 1541, 1751
MspCI	CTTAAG	1 cut(s)	1328
MspI	CCGG	1 cut(s)	2377
MspR9I	CCNGG	5 cut(s)	360, 768, 1729, 1845, 2378
Mva1269I	GAATGC	2 cut(s)	1799, 1826
MvaI	CCWGG	4 cut(s)	360, 768, 1729, 1845
MwoI	GCNNNNNNNGC	6 cut(s)	431, 600, 1283, 1538, 1877, 2053
NarI	GGCGCC	1 cut(s)	603
NciI	CCSGG	1 cut(s)	2378
NcoI	CCATGG	2 cut(s)	44, 606
NdeI	CATATG	1 cut(s)	1705
NdeII	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
NlaIV	GGNNCC	7 cut(s)	424, 425, 604, 841, 1005, 1158, 1400
NmuCI	GTSAC	4 cut(s)	362, 782, 1403, 1600
NsiI	ATGCAT	1 cut(s)	1828
NspI	RCATGY	3 cut(s)	1657, 1875, 2037
NspV	TTCGAA	1 cut(s)	169
OliI	CACNNNNGTG	1 cut(s)	56
PagI	TCATGA	2 cut(s)	1014, 1830
PceI	AGGCCT	1 cut(s)	1818
PciI	ACATGT	1 cut(s)	1653
PciSI	GCTCTTC	2 cut(s)	111, 2023
PcsI	WCGNNNNNNNCGW	1 cut(s)	160
PctI	GAATGC	2 cut(s)	1799, 1826
PdmI	GAANNNNTTC	1 cut(s)	1008
PfeI	GAWTC	4 cut(s)	628, 1194, 2063, 2116
PflMI	CCANNNNNTGG	1 cut(s)	1445
PkrI	GCNGC	5 cut(s)	470, 1489, 1540, 1807, 2140
PleI	GAGTC	3 cut(s)	325, 1666, 2297
PluTI	GGCGCC	1 cut(s)	606
PpsI	GAGTC	3 cut(s)	325, 1666, 2297
Ppu21I	YACGTR	2 cut(s)	1505, 1789
PscI	ACATGT	1 cut(s)	1653
PshBI	ATTAAT	3 cut(s)	194, 279, 2154
PsiI	TTATAA	1 cut(s)	480
Psp124BI	GAGCTC	1 cut(s)	127
Psp6I	CCWGG	4 cut(s)	358, 766, 1727, 1843
PspEI	GGTNACC	1 cut(s)	38
PspFI	CCCAGC	2 cut(s)	1260, 2073
PspGI	CCWGG	4 cut(s)	358, 766, 1727, 1843
PspN4I	GGNNCC	7 cut(s)	424, 425, 604, 841, 1005, 1158, 1400
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	7 cut(s)	374, 423, 424, 839, 1066, 1112, 1425
PsuI	RGATCY	4 cut(s)	462, 1156, 1250, 1718
PvuII	CAGCTG	3 cut(s)	1472, 1541, 1751
RseI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
SacI	GAGCTC	1 cut(s)	127
SapI	GCTCTTC	2 cut(s)	111, 2023
SaqAI	TTAA	7 cut(s)	194, 279, 1329, 1563, 1821, 1985, 2154
SatI	GCNGC	5 cut(s)	469, 1488, 1539, 1806, 2139
Sau3AI	GATC	8 cut(s)	187, 229, 462, 1156, 1250, 1543, 1718, 1740
Sau96I	GGNCC	7 cut(s)	374, 423, 424, 839, 1066, 1112, 1425
ScaI	AGTACT	2 cut(s)	687, 1572
SchI	GAGTC	3 cut(s)	325, 1666, 2298
ScrFI	CCNGG	5 cut(s)	360, 768, 1729, 1845, 2378
SduI	GDGCHC	3 cut(s)	127, 427, 2353
SfaNI	GCATC	4 cut(s)	421, 1546, 1858, 2140
SfcI	CTRYAG	2 cut(s)	787, 2326
SfoI	GGCGCC	1 cut(s)	604
SfuI	TTCGAA	1 cut(s)	169
SinI	GGWCC	4 cut(s)	374, 839, 1066, 1112
SmiMI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
SmlI	CTYRAG	2 cut(s)	146, 1328
SmoI	CTYRAG	2 cut(s)	146, 1328
SseBI	AGGCCT	1 cut(s)	1818
SsiI	CCGC	5 cut(s)	466, 469, 594, 1419, 2322
SspDI	GGCGCC	1 cut(s)	602
SspI	AATATT	1 cut(s)	1628
SspMI	CTAG	1 cut(s)	1992
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	1 cut(s)	1818
StyD4I	CCNGG	5 cut(s)	358, 766, 1727, 1843, 2376
StyI	CCWWGG	4 cut(s)	44, 606, 933, 2250
TaaI	ACNGT	8 cut(s)	638, 710, 747, 788, 1060, 1403, 1613, 2212
TaiI	ACGT	4 cut(s)	145, 975, 1507, 1791
TaqI	TCGA	7 cut(s)	93, 169, 329, 772, 1899, 2061, 2371
TatI	WGTACW	7 cut(s)	542, 685, 760, 1466, 1570, 1613, 2212
TauI	GCSGC	1 cut(s)	471
TfiI	GAWTC	4 cut(s)	628, 1194, 2063, 2116
Tru1I	TTAA	7 cut(s)	194, 279, 1329, 1563, 1821, 1985, 2154
Tru9I	TTAA	7 cut(s)	194, 279, 1329, 1563, 1821, 1985, 2154
TscAI	CASTG	4 cut(s)	726, 791, 1063, 1262
TseFI	GTSAC	4 cut(s)	362, 782, 1403, 1600
TseI	GCWGC	4 cut(s)	1487, 1538, 1805, 2138
Tsp45I	GTSAC	4 cut(s)	362, 782, 1403, 1600
TspRI	CASTG	4 cut(s)	726, 791, 1063, 1262
Van91I	CCANNNNNTGG	1 cut(s)	1445
Vha464I	CTTAAG	1 cut(s)	1328
VpaK11BI	GGWCC	4 cut(s)	374, 839, 1066, 1112
VspI	ATTAAT	3 cut(s)	194, 279, 2154
XapI	RAATTY	1 cut(s)	1162
XceI	RCATGY	3 cut(s)	1657, 1875, 2037
XcmI	CCANNNNNNNNNTGG	3 cut(s)	774, 1446, 2105
XmnI	GAANNNNTTC	1 cut(s)	1008
XspI	CTAG	1 cut(s)	1992
ZrmI	AGTACT	2 cut(s)	687, 1572
Zsp2I	ATGCAT	1 cut(s)	1828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.