Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Reverse (-)

52827614 .. 52836257

Gene Structure 8644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009437

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAAGAGATGGTAACCCATGGCTCACAAGTGTGAATAATCACATCGGGAGACAATTCTGGGAGTTCGACCCGAATTTCGGAACACCAGAAGAGCGAGCTCAGGTGGAAAAAGCACGTCTTGAGTTTCGTTACAATCGTTTTCAATCCAAACATAGTTCTGATATATTAATGAGACTTCAGTTTGCAAAGGAGAAAGAAAGTGATCGAATGGAGCTACCACCTCAGGTGAAAATAAAAAGTGAAGAAGAAATTAATGAAGAAGAAGTAGCAACCACATTAAGAAGGGGGTTGAGATTTTACTCGACTCTTCAGGCTGAGGATGGTTTCTGGCCTGGTGACTATGGCGGTCCTTTGTTTCTTCTACCTGGCTTGGTTATTGGATTATCTGTAACAGGGGCCCTTGATGCAATCTTGCCACACGAGCATTCAAAGGAGATCCGCCGCTATCTTTATTACCATCAGAACAAAGATGGAGGTTGGGGACTGCATATAGAAGGGCCAAGTACAATGTTTTGTACAGCTCTGTCCTATGTGAGTCTGAGATTGCTCGGAGAGGAGATGGACGGCGGTGATGGCGCCATGGAGAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCACCATCCCATCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATGAATGGAATGGTAACAATCCACTGCCTCCTGAATTGTGGCTTTTACCTTACTTTCTCCCAGTACACCCAGGTCGAATGTGGTGTCACTGCAGGATGGTATACCTGCCCATGTCATATTTATACGGCAAGAGATTTGTTGGTCCCATCAACGGCCTTATTTTATCCCTCAGAAGAGAGCTTTATACCTTTCCCTATCACCTGATTGATTGGGACGAAGCAAGAAATCTATGCGCCAAGGAGGATTTGTACTACCCACATCCCATTTTACAGGACGTGCTGTGGGGGTGTCTTCATAAGGTTGGGGAACCTCTTCTAATGAGATGGCCATTGTCTAAGCTCAGGCAAAAAGCTCTCACCACCGTAATGGACCACATACACTATGAGGATGAAAACACTCAGTATATCTGCATTGGACCTGTCAATAAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCATATAAGTGTCATCTTTCAAGGATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCCCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTTTCTACCAACCTTGTGAATGAATATGGTTCCGTGCTTAAGAAGGCGCACTATTTCATCAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTTAGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCCAGACAATGGTTGGCCTGTCTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTGCTATCACATATGCCCTGTGAAATTGTGGGAGAAGCAATATCAGCTGATCAACTGCATGATGCTGTTAATGTAGTATTATCCCTACAGAACAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCGTTATTGATTATCAGTACGTGGAATGCACATCAGCAGCAGTTGAAGGCCTTAAAGCATTCATGAAATTATATCCAGGGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATCCAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTAGCGGCAAGACCTATCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTGTCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTTTCATGCCAAAACAAGGTATACACAAATCTCGAAGGGAACAAACCACATATTGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGAGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAAATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAAAAATTGCATGATTAGTTACTCGGGCTACAGAAATATTTTTCCTATCTGGGCACTTGGAGAGTATCGAAACCGGGTACTGCTACTTCACAAATGTATCAATCAAATAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

770

Amino Acids

87.97

Weight (kDa)

6.67

Isoelectric Point (pI)

45.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	147 - 406	2.4e-36	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	418 - 756	1e-52	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	810
Acc65I	GGTACC	1 cut(s)	1398
AccB1I	GGYRCC	2 cut(s)	602, 1398
AccB7I	CCANNNNNTGG	1 cut(s)	1445
AccI	GTMKAC	2 cut(s)	798, 2013
AciI	CCGC	6 cut(s)	372, 466, 469, 594, 1419, 1899
AclWI	GGATC	7 cut(s)	457, 1164, 1202, 1245, 1258, 1617, 2101
AcoI	YGGCCR	1 cut(s)	1022
AcsI	RAATTY	2 cut(s)	100, 1162
AcuI	CTGAAG	4 cut(s)	32, 188, 320, 1494
AcyI	GRCGYC	1 cut(s)	603
AfiI	CCNNNNNNNGG	9 cut(s)	104, 664, 665, 848, 1260, 1261, 1445, 1632, 2008
AflII	CTTAAG	1 cut(s)	1328
AgsI	TTSAA	8 cut(s)	170, 456, 1191, 1283, 1483, 1718, 2081, 2194
AjiI	CACGTC	2 cut(s)	143, 973
AjnI	CCWGG	5 cut(s)	358, 391, 766, 1631, 1747
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	5 cut(s)	70, 193, 1365, 1462, 2094
AlwI	GGATC	7 cut(s)	457, 1164, 1202, 1245, 1258, 1617, 2101
Ama87I	CYCGRG	1 cut(s)	2218
AoxI	GGCC	8 cut(s)	356, 423, 524, 850, 1022, 1424, 1451, 1720
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	3 cut(s)	1487, 1709, 2123
ApoI	RAATTY	2 cut(s)	100, 1162
ArsI	GACNNNNNNTTYG	4 cut(s)	86, 118, 766, 798
AseI	ATTAAT	2 cut(s)	194, 279
Asp700I	GAANNNNTTC	1 cut(s)	1008
Asp718I	GGTACC	1 cut(s)	1398
AspLEI	GCGC	3 cut(s)	605, 932, 1339
AspS9I	GGNCC	8 cut(s)	374, 423, 424, 524, 839, 1066, 1112, 1425
AsuC2I	CCSGG	1 cut(s)	2270
AsuHPI	GGTGA	7 cut(s)	265, 374, 608, 626, 887, 1045, 2105
AsuNHI	GCTAGC	1 cut(s)	1895
AvaI	CYCGRG	1 cut(s)	2218
AvaII	GGWCC	4 cut(s)	374, 839, 1066, 1112
AxyI	CCTNAGG	1 cut(s)	249
BaeGI	GKGCMC	2 cut(s)	427, 2251
BaeI	ACNNNNGTAYC	2 cut(s)	2275, 2308
BalI	TGGCCA	1 cut(s)	1024
BamHI	GGATCC	1 cut(s)	1250
BanI	GGYRCC	2 cut(s)	602, 1398
BanII	GRGCYC	2 cut(s)	127, 427
BarI	GAAGNNNNNNTAC	2 cut(s)	2265, 2297
BauI	CACGAG	1 cut(s)	446
BbsI	GAAGAC	2 cut(s)	980, 2182
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	342
BbvI	GCAGC	3 cut(s)	1499, 1721, 2135
BceAI	ACGGC	3 cut(s)	607, 838, 865
BcgI	CGANNNNNNTGC	2 cut(s)	2095, 2129
BciT130I	CCWGG	5 cut(s)	360, 393, 768, 1633, 1749
BclI	TGATCA	3 cut(s)	1543, 1644, 2136
BcnI	CCSGG	1 cut(s)	2270
BcoDI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 2094
BfaI	CTAG	1 cut(s)	1896
BfmI	CTRYAG	3 cut(s)	787, 1580, 2224
BfoI	RGCGCY	1 cut(s)	606
BfrI	CTTAAG	1 cut(s)	1328
BfuAI	ACCTGC	1 cut(s)	810
BisI	GCNGC	5 cut(s)	469, 1488, 1710, 1900, 2124
BlsI	GCNGC	5 cut(s)	470, 1489, 1711, 1901, 2125
BmcAI	AGTACT	1 cut(s)	687
Bme1390I	CCNGG	6 cut(s)	360, 393, 768, 1633, 1749, 2270
Bme18I	GGWCC	4 cut(s)	374, 839, 1066, 1112
BmeT110I	CYCGRG	1 cut(s)	2218
BmgBI	CACGTC	2 cut(s)	143, 973
BmgT120I	GGNCC	8 cut(s)	374, 423, 424, 524, 839, 1066, 1112, 1425
BmiI	GGNNCC	8 cut(s)	424, 425, 604, 841, 1005, 1252, 1321, 1400
BmrFI	CCNGG	6 cut(s)	360, 393, 768, 1633, 1749, 2270
BmrI	ACTGGG	1 cut(s)	752
BmsI	GCATC	6 cut(s)	421, 1546, 1613, 1762, 1818, 2125
BmtI	GCTAGC	1 cut(s)	1899
BmuI	ACTGGG	1 cut(s)	752
BpiI	GAAGAC	2 cut(s)	980, 2182
BpmI	CTGGAG	1 cut(s)	1422
Bpu10I	CCTNAGC	3 cut(s)	126, 342, 1037
BpuEI	CTTGAG	1 cut(s)	167
BpuMI	CCSGG	1 cut(s)	2270
BsaAI	YACGTR	1 cut(s)	1693
BsaHI	GRCGYC	1 cut(s)	603
BsaI	GGTCTC	1 cut(s)	2094
BsaJI	CCNNGG	6 cut(s)	44, 606, 637, 766, 933, 1748
BsaXI	ACNNNNNCTCC	4 cut(s)	929, 959, 1659, 1689
Bsc4I	CCNNNNNNNGG	9 cut(s)	104, 664, 665, 848, 1260, 1261, 1445, 1632, 2008
Bse1I	ACTGG	3 cut(s)	758, 1400, 2059
Bse21I	CCTNAGG	1 cut(s)	249
Bse3DI	GCAATG	1 cut(s)	1597
BseBI	CCWGG	5 cut(s)	360, 393, 768, 1633, 1749
BseDI	CCNNGG	6 cut(s)	44, 606, 637, 766, 933, 1748
BseGI	GGATG	9 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 1809, 2063
BseLI	CCNNNNNNNGG	9 cut(s)	104, 664, 665, 848, 1260, 1261, 1445, 1632, 2008
BseMI	GCAATG	1 cut(s)	1597
BseMII	CTCAG	9 cut(s)	140, 263, 333, 557, 696, 880, 1051, 1109, 1473
BseNI	ACTGG	3 cut(s)	758, 1400, 2059
BseRI	GAGGAG	2 cut(s)	596, 657
BseSI	GKGCMC	2 cut(s)	427, 2251
BseXI	GCAGC	3 cut(s)	1499, 1721, 2135
BseYI	CCCAGC	2 cut(s)	1260, 1977
BshFI	GGCC	8 cut(s)	358, 425, 526, 852, 1024, 1426, 1453, 1722
BshNI	GGYRCC	2 cut(s)	602, 1398
BsiHKAI	GWGCWC	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	2218
BsiSI	CCGG	1 cut(s)	2269
BslFI	GGGAC	3 cut(s)	522, 825, 923
BslI	CCNNNNNNNGG	9 cut(s)	104, 664, 665, 848, 1260, 1261, 1445, 1632, 2008
BsmAI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 2094
BsmFI	GGGAC	3 cut(s)	522, 825, 923
BsmI	GAATGC	3 cut(s)	451, 1703, 1730
BsnI	GGCC	8 cut(s)	358, 425, 526, 852, 1024, 1426, 1453, 1722
Bso31I	GGTCTC	1 cut(s)	2094
BsoBI	CYCGRG	1 cut(s)	2218
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	3 cut(s)	127, 427, 2251
Bsp1407I	TGTACA	2 cut(s)	542, 1466
Bsp19I	CCATGG	2 cut(s)	44, 606
BspACI	CCGC	6 cut(s)	372, 466, 469, 594, 1419, 1899
BspANI	GGCC	8 cut(s)	358, 425, 526, 852, 1024, 1426, 1453, 1722
BspCNI	CTCAG	9 cut(s)	139, 262, 334, 558, 695, 879, 1050, 1108, 1472
BspHI	TCATGA	1 cut(s)	1734
BspLI	GGNNCC	8 cut(s)	424, 425, 604, 841, 1005, 1252, 1321, 1400
BspMAI	CTGCAG	1 cut(s)	791
BspMI	ACCTGC	1 cut(s)	810
BspOI	GCTAGC	1 cut(s)	1899
BspPI	GGATC	7 cut(s)	457, 1164, 1202, 1245, 1258, 1617, 2101
BspQI	GCTCTTC	2 cut(s)	111, 1927
BspT107I	GGYRCC	2 cut(s)	602, 1398
BspTI	CTTAAG	1 cut(s)	1328
BspTNI	GGTCTC	1 cut(s)	2094
BsrDI	GCAATG	1 cut(s)	1597
BsrGI	TGTACA	2 cut(s)	542, 1466
BsrI	ACTGG	3 cut(s)	758, 1400, 2059
BssECI	CCNNGG	6 cut(s)	44, 606, 637, 766, 933, 1748
BssNAI	GTATAC	2 cut(s)	799, 2014
BssNI	GRCGYC	1 cut(s)	603
BssSI	CACGAG	1 cut(s)	446
BssT1I	CCWWGG	3 cut(s)	44, 606, 933
Bst1107I	GTATAC	2 cut(s)	799, 2014
Bst2BI	CACGAG	1 cut(s)	446
Bst2UI	CCWGG	5 cut(s)	360, 393, 768, 1633, 1749
Bst4CI	ACNGT	3 cut(s)	638, 1060, 1403
Bst6I	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 1927
BstACI	GRCGYC	1 cut(s)	603
BstAFI	CTTAAG	1 cut(s)	1328
BstAPI	GCANNNNNTGC	1 cut(s)	1781
BstAUI	TGTACA	2 cut(s)	542, 1466
BstBAI	YACGTR	1 cut(s)	1693
BstC8I	GCNNGC	2 cut(s)	123, 1897
BstDSI	CCRYGG	3 cut(s)	44, 606, 637
BstEII	GGTNACC	1 cut(s)	38
BstF5I	GGATG	9 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 1809, 2063
BstH2I	RGCGCY	1 cut(s)	606
BstHHI	GCGC	3 cut(s)	605, 932, 1339
BstMAI	GTCTC	5 cut(s)	70, 193, 1365, 1462, 2094
BstMWI	GCNNNNNNNGC	8 cut(s)	431, 448, 600, 1283, 1538, 1781, 1957, 2069
BstNI	CCWGG	5 cut(s)	360, 393, 768, 1633, 1749
BstNSI	RCATGY	2 cut(s)	1779, 1941
BstPI	GGTNACC	1 cut(s)	38
BstSCI	CCNGG	6 cut(s)	358, 391, 766, 1631, 1747, 2268
BstSFI	CTRYAG	3 cut(s)	787, 1580, 2224
BstSLI	GKGCMC	2 cut(s)	427, 2251
BstV1I	GCAGC	3 cut(s)	1499, 1721, 2135
BstV2I	GAAGAC	2 cut(s)	980, 2182
BstX2I	RGATCY	3 cut(s)	462, 1250, 1622
BstXI	CCANNNNNNTGG	1 cut(s)	1063
BstYI	RGATCY	3 cut(s)	462, 1250, 1622
BstZ17I	GTATAC	2 cut(s)	799, 2014
Bsu36I	CCTNAGG	1 cut(s)	249
BsuRI	GGCC	8 cut(s)	358, 425, 526, 852, 1024, 1426, 1453, 1722
BtgI	CCRYGG	3 cut(s)	44, 606, 637
BtrI	CACGTC	2 cut(s)	143, 973
BtsCI	GGATG	9 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 1809, 2063
BtsI	GCAGTG	2 cut(s)	719, 784
BtsIMutI	CAGTG	3 cut(s)	719, 784, 2052
BveI	ACCTGC	1 cut(s)	810
Cac8I	GCNNGC	2 cut(s)	123, 1897
CciI	TCATGA	1 cut(s)	1734
CfoI	GCGC	3 cut(s)	605, 932, 1339
Cfr13I	GGNCC	8 cut(s)	374, 423, 424, 524, 839, 1066, 1112, 1425
DinI	GGCGCC	1 cut(s)	604
EaeI	YGGCCR	1 cut(s)	1022
Eam1104I	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 1927
EarI	CTCTTC	6 cut(s)	25, 111, 339, 865, 1014, 1927
EciI	GGCGGA	2 cut(s)	455, 1434
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	3 cut(s)	44, 606, 933
Eco147I	AGGCCT	1 cut(s)	1722
Eco24I	GRGCYC	2 cut(s)	127, 427
Eco31I	GGTCTC	1 cut(s)	2094
Eco32I	GATATC	1 cut(s)	1672
Eco47I	GGWCC	4 cut(s)	374, 839, 1066, 1112
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	4 cut(s)	32, 188, 320, 1494
Eco81I	CCTNAGG	1 cut(s)	249
Eco88I	CYCGRG	1 cut(s)	2218
Eco91I	GGTNACC	1 cut(s)	38
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	2 cut(s)	423, 424
EcoO65I	GGTNACC	1 cut(s)	38
EcoRI	GAATTC	1 cut(s)	1162
EcoRII	CCWGG	5 cut(s)	358, 391, 766, 1631, 1747
EcoRV	GATATC	1 cut(s)	1672
EcoT14I	CCWWGG	3 cut(s)	44, 606, 933
EcoT38I	GRGCYC	2 cut(s)	127, 427
EgeI	GGCGCC	1 cut(s)	604
EheI	GGCGCC	1 cut(s)	604
ErhI	CCWWGG	3 cut(s)	44, 606, 933
FalI	AAGNNNNNCTT	4 cut(s)	129, 161, 1275, 1307
FaqI	GGGAC	3 cut(s)	522, 825, 923
FauNDI	CATATG	2 cut(s)	1506, 1609
FbaI	TGATCA	3 cut(s)	1543, 1644, 2136
FblI	GTMKAC	2 cut(s)	798, 2013
Fnu4HI	GCNGC	5 cut(s)	469, 1488, 1710, 1900, 2124
FokI	GGATG	9 cut(s)	359, 641, 805, 942, 1097, 1277, 1434, 1796, 2070
FriOI	GRGCYC	2 cut(s)	127, 427
Fsp4HI	GCNGC	5 cut(s)	469, 1488, 1710, 1900, 2124
FspBI	CTAG	1 cut(s)	1896
GlaI	GCGC	3 cut(s)	604, 931, 1338
GluI	GCNGC	5 cut(s)	469, 1488, 1710, 1900, 2124
GsaI	CCCAGC	2 cut(s)	1264, 1981
GsuI	CTGGAG	1 cut(s)	1422
HaeII	RGCGCY	1 cut(s)	606
HaeIII	GGCC	8 cut(s)	358, 425, 526, 852, 1024, 1426, 1453, 1722
HapII	CCGG	1 cut(s)	2269
HhaI	GCGC	3 cut(s)	605, 932, 1339
Hin1I	GRCGYC	1 cut(s)	603
Hin6I	GCGC	3 cut(s)	603, 930, 1337
HinP1I	GCGC	3 cut(s)	603, 930, 1337
HinfI	GANTC	5 cut(s)	331, 562, 628, 1967, 2187
HpaII	CCGG	1 cut(s)	2269
HphI	GGTGA	7 cut(s)	265, 374, 608, 626, 887, 1045, 2105
Hpy166II	GTNNAC	4 cut(s)	763, 799, 2014, 2050
Hpy188III	TCNNGA	8 cut(s)	73, 146, 728, 1439, 1735, 1804, 1820, 2024
Hpy8I	GTNNAC	4 cut(s)	763, 799, 2014, 2050
HpyCH4III	ACNGT	3 cut(s)	638, 1060, 1403
HpyCH4IV	ACGT	3 cut(s)	142, 972, 1692
HpyF10VI	GCNNNNNNNGC	8 cut(s)	431, 448, 600, 1283, 1538, 1781, 1957, 2069
HpySE526I	ACGT	3 cut(s)	142, 972, 1692
Hsp92I	GRCGYC	1 cut(s)	603
HspAI	GCGC	3 cut(s)	603, 930, 1337
KasI	GGCGCC	1 cut(s)	602
KpnI	GGTACC	1 cut(s)	1402
Ksp22I	TGATCA	3 cut(s)	1543, 1644, 2136
LguI	GCTCTTC	2 cut(s)	111, 1927
LmnI	GCTCC	1 cut(s)	238
Lsp1109I	GCAGC	3 cut(s)	1499, 1721, 2135
LweI	GCATC	6 cut(s)	421, 1546, 1613, 1762, 1818, 2125
MaeI	CTAG	1 cut(s)	1896
MaeII	ACGT	3 cut(s)	142, 972, 1692
MflI	RGATCY	3 cut(s)	462, 1250, 1622
MhlI	GDGCHC	3 cut(s)	127, 427, 2251
MlsI	TGGCCA	1 cut(s)	1024
MluNI	TGGCCA	1 cut(s)	1024
Mly113I	GGCGCC	1 cut(s)	603
MlyI	GAGTC	3 cut(s)	325, 571, 2196
MmeI	TCCRAC	2 cut(s)	1617, 1837
Mox20I	TGGCCA	1 cut(s)	1024
MroXI	GAANNNNTTC	1 cut(s)	1008
MscI	TGGCCA	1 cut(s)	1024
MseI	TTAA	7 cut(s)	194, 279, 305, 1329, 1563, 1725, 1889
MslI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
Msp20I	TGGCCA	1 cut(s)	1024
MspA1I	CMGCKG	3 cut(s)	1472, 1541, 1655
MspCI	CTTAAG	1 cut(s)	1328
MspI	CCGG	1 cut(s)	2269
MspR9I	CCNGG	6 cut(s)	360, 393, 768, 1633, 1749, 2270
Mva1269I	GAATGC	3 cut(s)	451, 1703, 1730
MvaI	CCWGG	5 cut(s)	360, 393, 768, 1633, 1749
MwoI	GCNNNNNNNGC	8 cut(s)	431, 448, 600, 1283, 1538, 1781, 1957, 2069
NarI	GGCGCC	1 cut(s)	603
NciI	CCSGG	1 cut(s)	2270
NcoI	CCATGG	2 cut(s)	44, 606
NdeI	CATATG	2 cut(s)	1506, 1609
NheI	GCTAGC	1 cut(s)	1895
NlaIV	GGNNCC	8 cut(s)	424, 425, 604, 841, 1005, 1252, 1321, 1400
NmuCI	GTSAC	3 cut(s)	362, 782, 1403
NspI	RCATGY	2 cut(s)	1779, 1941
OliI	CACNNNNGTG	1 cut(s)	56
PagI	TCATGA	1 cut(s)	1734
PceI	AGGCCT	1 cut(s)	1722
PciSI	GCTCTTC	2 cut(s)	111, 1927
PcsI	WCGNNNNNNNCGW	1 cut(s)	160
PctI	GAATGC	3 cut(s)	451, 1703, 1730
PdmI	GAANNNNTTC	1 cut(s)	1008
PfeI	GAWTC	2 cut(s)	628, 1967
PflMI	CCANNNNNTGG	1 cut(s)	1445
PkrI	GCNGC	5 cut(s)	470, 1489, 1711, 1901, 2125
PleI	GAGTC	3 cut(s)	325, 570, 2195
PluTI	GGCGCC	1 cut(s)	606
PpsI	GAGTC	3 cut(s)	325, 570, 2195
Ppu21I	YACGTR	1 cut(s)	1693
PshBI	ATTAAT	2 cut(s)	194, 279
Psp124BI	GAGCTC	1 cut(s)	127
Psp6I	CCWGG	5 cut(s)	358, 391, 766, 1631, 1747
PspEI	GGTNACC	1 cut(s)	38
PspFI	CCCAGC	2 cut(s)	1260, 1977
PspGI	CCWGG	5 cut(s)	358, 391, 766, 1631, 1747
PspN4I	GGNNCC	8 cut(s)	424, 425, 604, 841, 1005, 1252, 1321, 1400
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	8 cut(s)	374, 423, 424, 524, 839, 1066, 1112, 1425
PstI	CTGCAG	1 cut(s)	791
PsuI	RGATCY	3 cut(s)	462, 1250, 1622
PvuII	CAGCTG	3 cut(s)	1472, 1541, 1655
RseI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
SacI	GAGCTC	1 cut(s)	127
SapI	GCTCTTC	2 cut(s)	111, 1927
SaqAI	TTAA	7 cut(s)	194, 279, 305, 1329, 1563, 1725, 1889
SatI	GCNGC	5 cut(s)	469, 1488, 1710, 1900, 2124
Sau96I	GGNCC	8 cut(s)	374, 423, 424, 524, 839, 1066, 1112, 1425
ScaI	AGTACT	1 cut(s)	687
SchI	GAGTC	3 cut(s)	325, 571, 2196
ScrFI	CCNGG	6 cut(s)	360, 393, 768, 1633, 1749, 2270
SduI	GDGCHC	3 cut(s)	127, 427, 2251
SfaNI	GCATC	6 cut(s)	421, 1546, 1613, 1762, 1818, 2125
SfcI	CTRYAG	3 cut(s)	787, 1580, 2224
SfoI	GGCGCC	1 cut(s)	604
SinI	GGWCC	4 cut(s)	374, 839, 1066, 1112
SmiMI	CAYNNNNRTG	3 cut(s)	56, 1061, 1177
SmlI	CTYRAG	2 cut(s)	146, 1328
SmoI	CTYRAG	2 cut(s)	146, 1328
SseBI	AGGCCT	1 cut(s)	1722
SsiI	CCGC	6 cut(s)	372, 466, 469, 594, 1419, 1899
SspDI	GGCGCC	1 cut(s)	602
SspI	AATATT	1 cut(s)	2233
SspMI	CTAG	1 cut(s)	1896
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	1 cut(s)	1722
StyD4I	CCNGG	6 cut(s)	358, 391, 766, 1631, 1747, 2268
StyI	CCWWGG	3 cut(s)	44, 606, 933
TaaI	ACNGT	3 cut(s)	638, 1060, 1403
TaiI	ACGT	3 cut(s)	145, 975, 1695
TaqI	TCGA	9 cut(s)	93, 232, 329, 772, 1803, 1965, 2025, 2119, 2263
TatI	WGTACW	6 cut(s)	530, 542, 685, 760, 945, 1466
TauI	GCSGC	2 cut(s)	471, 1902
TfiI	GAWTC	2 cut(s)	628, 1967
Tru1I	TTAA	7 cut(s)	194, 279, 305, 1329, 1563, 1725, 1889
Tru9I	TTAA	7 cut(s)	194, 279, 305, 1329, 1563, 1725, 1889
TscAI	CASTG	3 cut(s)	726, 791, 2059
TseFI	GTSAC	3 cut(s)	362, 782, 1403
TseI	GCWGC	3 cut(s)	1487, 1709, 2123
Tsp45I	GTSAC	3 cut(s)	362, 782, 1403
TspGWI	ACGGA	1 cut(s)	1312
TspRI	CASTG	3 cut(s)	726, 791, 2059
Van91I	CCANNNNNTGG	1 cut(s)	1445
Vha464I	CTTAAG	1 cut(s)	1328
VpaK11BI	GGWCC	4 cut(s)	374, 839, 1066, 1112
VspI	ATTAAT	2 cut(s)	194, 279
XapI	RAATTY	2 cut(s)	100, 1162
XceI	RCATGY	2 cut(s)	1779, 1941
XcmI	CCANNNNNNNNNTGG	3 cut(s)	774, 1446, 1820
XmiI	GTMKAC	2 cut(s)	798, 2013
XmnI	GAANNNNTTC	1 cut(s)	1008
XspI	CTAG	1 cut(s)	1896
ZrmI	AGTACT	1 cut(s)	687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.