Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

13030357 .. 13052195

Gene Structure 21839 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G006340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAAGAGATGGTAATCCATGGCTCACAAGTGTGAATAATCACATCGGGAGACAATTCTGGGAGTTCGACCCGAATCTCGGAACACCGGAAGAGCGAGTTCAAGTGGAAAAGCACGTCTTAAGTTTCATAACAATCGTTTTCGATCCAAACATATTTGCAAAGGAGAAAGAAAGTGATCGAATGGAGCTACCAGCTCAGGTGAAAATAAAAAGTGAAGAAGAAATTAATGAAGAAGTAGTAGCAACCACATTGAGGAGGGGGTTGAGATTTTACTCGACTCTTCAGGCTGAGGATGGTTTATGGCCTGATGACCTTGGCGGTCCTTTGTTTCTTCTACCTGGCTTGGTTATTGGATTATCTGTAACAGGGGACCTTGATGCAATCTTGTCACACGAGCATTCAAAGGAGATCCGCCGCTATCTTTATAACCATCAGAACAAAGATGGTGGATGGGGACTCCATATAGAAGGGCCAAGTACAATGTTTTGTACAGCTCTGTCCTATGTGAGTCTGAGATTGCTCGGAGAGGAGATGGACGGCGGTGAGGACACCATGGAGAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCACCATCCCATCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATGAATGGGATGGTAACAATCCACTGCCTCCTGAATTGTGGCTTTTACCTTACTTTCTCCCAGTACACCCAGGTCGAATGTGGTGTCATTGTCGGATGGTATACCTTCCCATGTCATATTTATACGGCAAAAGATTTGTTGGTCCCATCGACGGCCTTATTCTATCCCTCAGAAGAGAGCTTTATACCCTTCCCTATCACTTGATTGATTGGGACGAAGCAAGAAATCTATGCGCCAAGGAGGATTTGTATTATCCACATCCCATTTTACAGGACGTGCTCTGGGGGTGTCTTCATAAGGTTGGGGAACCTCTTCTCATGAGATGGCCATTGTCTAACCTCAGGCAAAAAGCTCTCAACACTGTAATGGACCACATACACTATGAGGATGAAAACACTCAGTATATCTGCATTGGACCTGTCAATAAGGTACTAAACATGATCTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCATATAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCCCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTTTCTACCAACCTTGTGAATGAATATGGTTCCGTGCTTAAGAAGGCGCACTATTTCATAAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTTAGTTACTGGTACCGTCACATCTCTAAGGGCGGATGGCCCTTCTCCACTCAAGACAATGGTTGGCCTGTCTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTATTATCACGTTTGCCCTGCGAAATTGTGGGAGAAGCAATAGCAGCTGATCAACTGTATGATGCTGTTAATGTTGTATTATCCCTACAGAACAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCGTTATTGATTATCAGTACGTGGAATGCACATCAGCAGCAGTTGAATGCCTTAAAGCATTCATGAAATTATATCCAGAGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATGCAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTATTGGCTAGTGGCAAGACCTACCTGACAAGTAACAGCATTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTTTCATGCCAAAACAAGGTATACACAAATCTCGAAGGGAACAAACCACATATAGTGAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATTAATTCACAGATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGTGTCTTCAGCAAAAATTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTGGAGAGTATCGAAACCGGGTACTGGTACTTCACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

754

Amino Acids

86.08

Weight (kDa)

5.91

Isoelectric Point (pI)

45.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	103 - 397	1.4e-37	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	409 - 747	1e-53	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	453
Acc65I	GGTACC	1 cut(s)	1371
AccB1I	GGYRCC	1 cut(s)	1371
AccI	GTMKAC	2 cut(s)	771, 1986
AciI	CCGC	6 cut(s)	345, 439, 442, 567, 1392, 2193
AclWI	GGATC	7 cut(s)	164, 430, 1137, 1218, 1231, 1590, 2074
AcoI	YGGCCR	1 cut(s)	995
AcsI	RAATTY	1 cut(s)	1135
AcuI	CTGAAG	4 cut(s)	32, 293, 1467, 2149
AflII	CTTAAG	2 cut(s)	145, 1301
AgsI	TTSAA	7 cut(s)	128, 429, 1164, 1256, 1456, 1691, 2054
AjiI	CACGTC	2 cut(s)	142, 946
AjnI	CCWGG	3 cut(s)	364, 739, 1604
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	1 cut(s)	951
Alw26I	GTCTC	3 cut(s)	70, 1338, 1435
AlwI	GGATC	7 cut(s)	164, 430, 1137, 1218, 1231, 1590, 2074
AoxI	GGCC	6 cut(s)	329, 497, 823, 995, 1397, 1424
ApeKI	GCWGC	4 cut(s)	1460, 1511, 1682, 2096
ApoI	RAATTY	1 cut(s)	1135
ArsI	GACNNNNNNTTYG	2 cut(s)	739, 771
AseI	ATTAAT	2 cut(s)	252, 2112
Asp700I	GAANNNNTTC	1 cut(s)	981
Asp718I	GGTACC	1 cut(s)	1371
AspLEI	GCGC	2 cut(s)	905, 1312
AspS9I	GGNCC	8 cut(s)	347, 397, 497, 812, 1039, 1085, 1398, 2074
AsuC2I	CCSGG	1 cut(s)	2243
AsuHPI	GGTGA	4 cut(s)	238, 581, 599, 2078
AvaII	GGWCC	6 cut(s)	347, 397, 812, 1039, 1085, 2074
AxyI	CCTNAGG	1 cut(s)	1010
BaeGI	GKGCMC	1 cut(s)	2224
BalI	TGGCCA	1 cut(s)	997
BamHI	GGATCC	1 cut(s)	1223
BanI	GGYRCC	1 cut(s)	1371
BarI	GAAGNNNNNNTAC	1 cut(s)	2238
BauI	CACGAG	1 cut(s)	419
BbsI	GAAGAC	2 cut(s)	953, 2155
Bbv12I	GWGCWC	1 cut(s)	951
BbvCI	CCTCAGC	1 cut(s)	315
BbvI	GCAGC	4 cut(s)	1472, 1523, 1694, 2108
BceAI	ACGGC	3 cut(s)	580, 811, 838
BcgI	CGANNNNNNTGC	2 cut(s)	2068, 2102
BciT130I	CCWGG	3 cut(s)	366, 741, 1606
BclI	TGATCA	2 cut(s)	1516, 1617
BcnI	CCSGG	1 cut(s)	2243
BcoDI	GTCTC	3 cut(s)	70, 1338, 1435
BfaI	CTAG	1 cut(s)	1869
BfmI	CTRYAG	2 cut(s)	1553, 2197
BfrI	CTTAAG	2 cut(s)	145, 1301
BisI	GCNGC	5 cut(s)	442, 1461, 1512, 1683, 2097
BlsI	GCNGC	5 cut(s)	443, 1462, 1513, 1684, 2098
BmcAI	AGTACT	1 cut(s)	660
Bme1390I	CCNGG	4 cut(s)	366, 741, 1606, 2243
Bme18I	GGWCC	6 cut(s)	347, 397, 812, 1039, 1085, 2074
BmgBI	CACGTC	2 cut(s)	142, 946
BmgT120I	GGNCC	8 cut(s)	347, 397, 497, 812, 1039, 1085, 1398, 2074
BmiI	GGNNCC	8 cut(s)	398, 814, 978, 1225, 1294, 1373, 2075, 2076
BmrFI	CCNGG	4 cut(s)	366, 741, 1606, 2243
BmrI	ACTGGG	1 cut(s)	725
BmsI	GCATC	5 cut(s)	394, 1519, 1586, 1735, 2098
BmuI	ACTGGG	1 cut(s)	725
BpiI	GAAGAC	2 cut(s)	953, 2155
Bpu10I	CCTNAGC	2 cut(s)	222, 315
BpuEI	CTTGAG	1 cut(s)	1395
BpuMI	CCSGG	1 cut(s)	2243
BsaAI	YACGTR	1 cut(s)	1666
BsaBI	GATNNNNATC	1 cut(s)	39
BsaJI	CCNNGG	6 cut(s)	44, 340, 579, 610, 739, 906
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	4 cut(s)	902, 932, 1632, 1662
Bse1I	ACTGG	4 cut(s)	731, 1373, 2032, 2253
Bse21I	CCTNAGG	1 cut(s)	1010
Bse3DI	GCAATG	1 cut(s)	1570
Bse8I	GATNNNNATC	1 cut(s)	39
BseBI	CCWGG	3 cut(s)	366, 741, 1606
BseDI	CCNNGG	6 cut(s)	44, 340, 579, 610, 739, 906
BseJI	GATNNNNATC	1 cut(s)	39
BseMI	GCAATG	1 cut(s)	1570
BseMII	CTCAG	8 cut(s)	236, 306, 530, 669, 853, 1024, 1082, 1446
BseNI	ACTGG	4 cut(s)	731, 1373, 2032, 2253
BseRI	GAGGAG	3 cut(s)	295, 569, 630
BseSI	GKGCMC	1 cut(s)	2224
BseXI	GCAGC	4 cut(s)	1472, 1523, 1694, 2108
BseYI	CCCAGC	2 cut(s)	1233, 1950
BshFI	GGCC	6 cut(s)	331, 499, 825, 997, 1399, 1426
BshNI	GGYRCC	1 cut(s)	1371
BsiHKAI	GWGCWC	1 cut(s)	951
BsiSI	CCGG	2 cut(s)	113, 2242
BslFI	GGGAC	5 cut(s)	410, 495, 798, 896, 2087
BsmAI	GTCTC	3 cut(s)	70, 1338, 1435
BsmFI	GGGAC	5 cut(s)	410, 495, 798, 896, 2087
BsmI	GAATGC	4 cut(s)	424, 1676, 1697, 1703
BsnI	GGCC	6 cut(s)	331, 499, 825, 997, 1399, 1426
Bsp1286I	GDGCHC	2 cut(s)	951, 2224
Bsp1407I	TGTACA	2 cut(s)	515, 1439
Bsp19I	CCATGG	2 cut(s)	44, 579
BspACI	CCGC	6 cut(s)	345, 439, 442, 567, 1392, 2193
BspANI	GGCC	6 cut(s)	331, 499, 825, 997, 1399, 1426
BspCNI	CTCAG	8 cut(s)	235, 307, 531, 668, 852, 1023, 1081, 1445
BspHI	TCATGA	2 cut(s)	987, 1707
BspLI	GGNNCC	8 cut(s)	398, 814, 978, 1225, 1294, 1373, 2075, 2076
BspPI	GGATC	7 cut(s)	164, 430, 1137, 1218, 1231, 1590, 2074
BspQI	GCTCTTC	2 cut(s)	111, 1900
BspT107I	GGYRCC	1 cut(s)	1371
BspTI	CTTAAG	2 cut(s)	145, 1301
BsrDI	GCAATG	1 cut(s)	1570
BsrGI	TGTACA	2 cut(s)	515, 1439
BsrI	ACTGG	4 cut(s)	731, 1373, 2032, 2253
BssECI	CCNNGG	6 cut(s)	44, 340, 579, 610, 739, 906
BssNAI	GTATAC	2 cut(s)	772, 1987
BssSI	CACGAG	1 cut(s)	419
BssT1I	CCWWGG	4 cut(s)	44, 340, 579, 906
Bst1107I	GTATAC	2 cut(s)	772, 1987
Bst2BI	CACGAG	1 cut(s)	419
Bst2UI	CCWGG	3 cut(s)	366, 741, 1606
Bst4CI	ACNGT	4 cut(s)	611, 1033, 1376, 1524
Bst6I	CTCTTC	6 cut(s)	25, 111, 312, 838, 987, 1900
BstAFI	CTTAAG	2 cut(s)	145, 1301
BstAPI	GCANNNNNTGC	2 cut(s)	1754, 2175
BstAUI	TGTACA	2 cut(s)	515, 1439
BstBAI	YACGTR	1 cut(s)	1666
BstC8I	GCNNGC	1 cut(s)	1784
BstDSI	CCRYGG	3 cut(s)	44, 579, 610
BstHHI	GCGC	2 cut(s)	905, 1312
BstMAI	GTCTC	3 cut(s)	70, 1338, 1435
BstMWI	GCNNNNNNNGC	6 cut(s)	1256, 1511, 1754, 1930, 2042, 2175
BstNI	CCWGG	3 cut(s)	366, 741, 1606
BstNSI	RCATGY	3 cut(s)	1752, 1786, 1914
BstSCI	CCNGG	4 cut(s)	364, 739, 1604, 2241
BstSFI	CTRYAG	2 cut(s)	1553, 2197
BstSLI	GKGCMC	1 cut(s)	2224
BstV1I	GCAGC	4 cut(s)	1472, 1523, 1694, 2108
BstV2I	GAAGAC	2 cut(s)	953, 2155
BstX2I	RGATCY	3 cut(s)	435, 1223, 1595
BstYI	RGATCY	3 cut(s)	435, 1223, 1595
BstZ17I	GTATAC	2 cut(s)	772, 1987
Bsu36I	CCTNAGG	1 cut(s)	1010
BsuRI	GGCC	6 cut(s)	331, 499, 825, 997, 1399, 1426
BtgI	CCRYGG	3 cut(s)	44, 579, 610
BtrI	CACGTC	2 cut(s)	142, 946
BtsI	GCAGTG	1 cut(s)	692
BtsIMutI	CAGTG	3 cut(s)	692, 1029, 2025
Cac8I	GCNNGC	1 cut(s)	1784
CciI	TCATGA	2 cut(s)	987, 1707
CfoI	GCGC	2 cut(s)	905, 1312
Cfr13I	GGNCC	8 cut(s)	347, 397, 497, 812, 1039, 1085, 1398, 2074
CspCI	CAANNNNNGTGG	2 cut(s)	454, 489
EaeI	YGGCCR	1 cut(s)	995
Eam1104I	CTCTTC	6 cut(s)	25, 111, 312, 838, 987, 1900
EarI	CTCTTC	6 cut(s)	25, 111, 312, 838, 987, 1900
EciI	GGCGGA	3 cut(s)	428, 1407, 2182
Eco130I	CCWWGG	4 cut(s)	44, 340, 579, 906
Eco32I	GATATC	1 cut(s)	1645
Eco47I	GGWCC	6 cut(s)	347, 397, 812, 1039, 1085, 2074
Eco57I	CTGAAG	4 cut(s)	32, 293, 1467, 2149
Eco81I	CCTNAGG	1 cut(s)	1010
EcoO109I	RGGNCCY	2 cut(s)	397, 2074
EcoRI	GAATTC	1 cut(s)	1135
EcoRII	CCWGG	3 cut(s)	364, 739, 1604
EcoRV	GATATC	1 cut(s)	1645
EcoT14I	CCWWGG	4 cut(s)	44, 340, 579, 906
ErhI	CCWWGG	4 cut(s)	44, 340, 579, 906
FalI	AAGNNNNNCTT	4 cut(s)	128, 160, 1248, 1280
FaqI	GGGAC	5 cut(s)	410, 495, 798, 896, 2087
FauNDI	CATATG	1 cut(s)	1582
FbaI	TGATCA	2 cut(s)	1516, 1617
FblI	GTMKAC	2 cut(s)	771, 1986
Fnu4HI	GCNGC	5 cut(s)	442, 1461, 1512, 1683, 2097
Fsp4HI	GCNGC	5 cut(s)	442, 1461, 1512, 1683, 2097
FspBI	CTAG	1 cut(s)	1869
GlaI	GCGC	2 cut(s)	904, 1311
GluI	GCNGC	5 cut(s)	442, 1461, 1512, 1683, 2097
GsaI	CCCAGC	2 cut(s)	1237, 1954
HaeIII	GGCC	6 cut(s)	331, 499, 825, 997, 1399, 1426
HapII	CCGG	2 cut(s)	113, 2242
HhaI	GCGC	2 cut(s)	905, 1312
Hin6I	GCGC	2 cut(s)	903, 1310
HinP1I	GCGC	2 cut(s)	903, 1310
HinfI	GANTC	7 cut(s)	100, 304, 483, 535, 601, 1167, 1940
HpaII	CCGG	2 cut(s)	113, 2242
HphI	GGTGA	4 cut(s)	238, 581, 599, 2078
Hpy166II	GTNNAC	4 cut(s)	736, 772, 1987, 2023
Hpy8I	GTNNAC	4 cut(s)	736, 772, 1987, 2023
Hpy99I	CGWCG	1 cut(s)	824
HpyCH4III	ACNGT	4 cut(s)	611, 1033, 1376, 1524
HpyCH4IV	ACGT	4 cut(s)	141, 945, 1477, 1665
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1256, 1511, 1754, 1930, 2042, 2175
HpySE526I	ACGT	4 cut(s)	141, 945, 1477, 1665
HspAI	GCGC	2 cut(s)	903, 1310
KflI	GGGWCCC	1 cut(s)	2074
KpnI	GGTACC	1 cut(s)	1375
Ksp22I	TGATCA	2 cut(s)	1516, 1617
LguI	GCTCTTC	2 cut(s)	111, 1900
LmnI	GCTCC	1 cut(s)	211
Lsp1109I	GCAGC	4 cut(s)	1472, 1523, 1694, 2108
LweI	GCATC	5 cut(s)	394, 1519, 1586, 1735, 2098
MaeI	CTAG	1 cut(s)	1869
MaeII	ACGT	4 cut(s)	141, 945, 1477, 1665
MaeIII	GTNAC	8 cut(s)	388, 414, 619, 683, 1364, 1376, 1892, 2186
MflI	RGATCY	3 cut(s)	435, 1223, 1595
MhlI	GDGCHC	2 cut(s)	951, 2224
MlsI	TGGCCA	1 cut(s)	997
MluCI	AATT	9 cut(s)	80, 249, 704, 1135, 1491, 1712, 2049, 2113, 2173
MluNI	TGGCCA	1 cut(s)	997
MlyI	GAGTC	3 cut(s)	298, 477, 544
MmeI	TCCRAC	2 cut(s)	743, 1590
Mox20I	TGGCCA	1 cut(s)	997
MroXI	GAANNNNTTC	1 cut(s)	981
MscI	TGGCCA	1 cut(s)	997
MseI	TTAA	6 cut(s)	146, 252, 1302, 1536, 1698, 2112
MslI	CAYNNNNRTG	3 cut(s)	56, 1034, 1150
Msp20I	TGGCCA	1 cut(s)	997
MspA1I	CMGCKG	3 cut(s)	1445, 1514, 1628
MspCI	CTTAAG	2 cut(s)	145, 1301
MspI	CCGG	2 cut(s)	113, 2242
MspR9I	CCNGG	4 cut(s)	366, 741, 1606, 2243
Mva1269I	GAATGC	4 cut(s)	424, 1676, 1697, 1703
MvaI	CCWGG	3 cut(s)	366, 741, 1606
MwoI	GCNNNNNNNGC	6 cut(s)	1256, 1511, 1754, 1930, 2042, 2175
NciI	CCSGG	1 cut(s)	2243
NcoI	CCATGG	2 cut(s)	44, 579
NdeI	CATATG	1 cut(s)	1582
NlaIV	GGNNCC	8 cut(s)	398, 814, 978, 1225, 1294, 1373, 2075, 2076
NmuCI	GTSAC	2 cut(s)	414, 1376
NspI	RCATGY	3 cut(s)	1752, 1786, 1914
OliI	CACNNNNGTG	1 cut(s)	56
PaeI	GCATGC	1 cut(s)	1786
PagI	TCATGA	2 cut(s)	987, 1707
PciSI	GCTCTTC	2 cut(s)	111, 1900
PctI	GAATGC	4 cut(s)	424, 1676, 1697, 1703
PdmI	GAANNNNTTC	1 cut(s)	981
PfeI	GAWTC	4 cut(s)	100, 601, 1167, 1940
PkrI	GCNGC	5 cut(s)	443, 1462, 1513, 1684, 2098
PleI	GAGTC	3 cut(s)	298, 477, 543
PpsI	GAGTC	3 cut(s)	298, 477, 543
Ppu21I	YACGTR	1 cut(s)	1666
PpuMI	RGGWCCY	2 cut(s)	397, 2074
PshBI	ATTAAT	2 cut(s)	252, 2112
PsiI	TTATAA	1 cut(s)	453
Psp5II	RGGWCCY	2 cut(s)	397, 2074
Psp6I	CCWGG	3 cut(s)	364, 739, 1604
PspFI	CCCAGC	2 cut(s)	1233, 1950
PspGI	CCWGG	3 cut(s)	364, 739, 1604
PspN4I	GGNNCC	8 cut(s)	398, 814, 978, 1225, 1294, 1373, 2075, 2076
PspPI	GGNCC	8 cut(s)	347, 397, 497, 812, 1039, 1085, 1398, 2074
PspPPI	RGGWCCY	2 cut(s)	397, 2074
PsuI	RGATCY	3 cut(s)	435, 1223, 1595
PvuII	CAGCTG	3 cut(s)	1445, 1514, 1628
RseI	CAYNNNNRTG	3 cut(s)	56, 1034, 1150
SapI	GCTCTTC	2 cut(s)	111, 1900
SaqAI	TTAA	6 cut(s)	146, 252, 1302, 1536, 1698, 2112
SatI	GCNGC	5 cut(s)	442, 1461, 1512, 1683, 2097
Sau96I	GGNCC	8 cut(s)	347, 397, 497, 812, 1039, 1085, 1398, 2074
ScaI	AGTACT	1 cut(s)	660
SchI	GAGTC	3 cut(s)	298, 477, 544
ScrFI	CCNGG	4 cut(s)	366, 741, 1606, 2243
SduI	GDGCHC	2 cut(s)	951, 2224
SfaNI	GCATC	5 cut(s)	394, 1519, 1586, 1735, 2098
SfcI	CTRYAG	2 cut(s)	1553, 2197
SinI	GGWCC	6 cut(s)	347, 397, 812, 1039, 1085, 2074
SmiMI	CAYNNNNRTG	3 cut(s)	56, 1034, 1150
SmlI	CTYRAG	3 cut(s)	145, 1301, 1410
SmoI	CTYRAG	3 cut(s)	145, 1301, 1410
SphI	GCATGC	1 cut(s)	1786
Sse9I	AATT	9 cut(s)	80, 249, 704, 1135, 1491, 1712, 2049, 2113, 2173
SsiI	CCGC	6 cut(s)	345, 439, 442, 567, 1392, 2193
SspMI	CTAG	1 cut(s)	1869
StyD4I	CCNGG	4 cut(s)	364, 739, 1604, 2241
StyI	CCWWGG	4 cut(s)	44, 340, 579, 906
TaaI	ACNGT	4 cut(s)	611, 1033, 1376, 1524
TaiI	ACGT	4 cut(s)	144, 948, 1480, 1668
TasI	AATT	9 cut(s)	80, 249, 704, 1135, 1491, 1712, 2049, 2113, 2173
TatI	WGTACW	5 cut(s)	503, 515, 658, 733, 1439
TauI	GCSGC	1 cut(s)	444
TfiI	GAWTC	4 cut(s)	100, 601, 1167, 1940
Tru1I	TTAA	6 cut(s)	146, 252, 1302, 1536, 1698, 2112
Tru9I	TTAA	6 cut(s)	146, 252, 1302, 1536, 1698, 2112
TscAI	CASTG	3 cut(s)	699, 1036, 2032
TseFI	GTSAC	2 cut(s)	414, 1376
TseI	GCWGC	4 cut(s)	1460, 1511, 1682, 2096
Tsp45I	GTSAC	2 cut(s)	414, 1376
TspGWI	ACGGA	1 cut(s)	1285
TspRI	CASTG	3 cut(s)	699, 1036, 2032
Vha464I	CTTAAG	2 cut(s)	145, 1301
VpaK11BI	GGWCC	6 cut(s)	347, 397, 812, 1039, 1085, 2074
VspI	ATTAAT	2 cut(s)	252, 2112
XapI	RAATTY	1 cut(s)	1135
XceI	RCATGY	3 cut(s)	1752, 1786, 1914
XcmI	CCANNNNNNNNNTGG	1 cut(s)	747
XmiI	GTMKAC	2 cut(s)	771, 1986
XmnI	GAANNNNTTC	1 cut(s)	981
XspI	CTAG	1 cut(s)	1869
ZrmI	AGTACT	1 cut(s)	660

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.