Rroxscaffold_5G00342320

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005243
Physical Location & Seq
Forward (+)
11127451 .. 11130560
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342320.1

Sequence Viewer

Length: 1278 bp
ATGAGATGGCCATTGTCTAACCTCAGGCAAAAAGCTCTCACCACTGTGATGGACCACATACACTACGAGGATGAAAACACTCAGTATATCTGCATTGGAGCTGTCAATAAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCGTACAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATCGAATGGATCTCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTCTCTACCAACCTTGTGAATGAATATGGTTCCGTGCTTAAGAAGGCGCACTATTTCATCAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTGCGTTACTGGTACCGTCACATATCTAAGGGCGGATGGCCCTTCTCCACTATAGACAATGGTTGGCCTGCTTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTATTATCACGTATGCCCTGCGAAATAGTGGGAGAAGCAATAGCAGCTGATCAACTGCTTGATGCTGTTAATGTAGTATTATCCCTACAGAACAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATAATCAATCCAGCTGAAACATTTGGAGATATCATGACTGATTATCAGTATGTGGAATGCACATCATCAGCAGTTGAAGGCCTTAATGCATTCATGAAATTATATCCAGGGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATACAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTGGTGGCAAGACCTATCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTCTCATGCCAAAACAAGGTATACACAAATCTCGAAGGGAACAAACCACATATAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAGATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAAAAACTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTGGAGAGTATCGAAACCGGGTATTGGTACTTCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

425

Amino Acids

47.96

Weight (kDa)

6.79

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 80 - 418 3.7e-53 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 999
AciI CCGC 2 cut(s) 405, 1206
AclWI GGATC 5 cut(s) 150, 235, 244, 603, 1087
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 1 cut(s) 148
AcuI CTGAAG 2 cut(s) 429, 480
AfaI GTAC 5 cut(s) 114, 161, 386, 454, 1266
AfiI CCNNNNNNNGG 3 cut(s) 618, 994, 1261
AflII CTTAAG 1 cut(s) 314
AgsI TTSAA 6 cut(s) 177, 269, 469, 704, 1067, 1180
AjnI CCWGG 2 cut(s) 617, 733
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 7 cut(s) 35, 101, 363, 458, 527, 641, 975
AluI AGCT 7 cut(s) 35, 101, 363, 458, 527, 641, 975
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 5 cut(s) 150, 235, 244, 603, 1087
AoxI GGCC 4 cut(s) 8, 410, 437, 706
ApeKI GCWGC 3 cut(s) 473, 524, 1109
ApoI RAATTY 1 cut(s) 148
Asp718I GGTACC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 3 cut(s) 52, 411, 1087
AsuC2I CCSGG 1 cut(s) 1256
AsuHPI GGTGA 2 cut(s) 31, 1091
AvaII GGWCC 2 cut(s) 52, 1087
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 10
BanI GGYRCC 1 cut(s) 384
BarI GAAGNNNNNNTAC 1 cut(s) 1251
BbsI GAAGAC 1 cut(s) 1168
BbvI GCAGC 3 cut(s) 485, 536, 1121
BccI CCATC 7 cut(s) 43, 203, 402, 803, 1038, 1129, 1235
BcgI CGANNNNNNTGC 2 cut(s) 1081, 1115
BciT130I CCWGG 2 cut(s) 619, 735
BclI TGATCA 2 cut(s) 529, 1122
BcnI CCSGG 1 cut(s) 1256
BcoDI GTCTC 1 cut(s) 351
BfmI CTRYAG 3 cut(s) 423, 566, 1210
BfrI CTTAAG 1 cut(s) 314
BisI GCNGC 3 cut(s) 474, 525, 1110
BlsI GCNGC 3 cut(s) 475, 526, 1111
Bme1390I CCNGG 3 cut(s) 619, 735, 1256
Bme18I GGWCC 2 cut(s) 52, 1087
BmgT120I GGNCC 3 cut(s) 52, 411, 1087
BmiI GGNNCC 4 cut(s) 307, 386, 1088, 1089
BmrFI CCNGG 3 cut(s) 619, 735, 1256
BmsI GCATC 4 cut(s) 532, 599, 748, 1111
BpiI GAAGAC 1 cut(s) 1168
BpuMI CCSGG 1 cut(s) 1256
BsaAI YACGTR 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 734
Bsc4I CCNNNNNNNGG 3 cut(s) 618, 994, 1261
Bse1I ACTGG 2 cut(s) 386, 1045
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 583
BseBI CCWGG 2 cut(s) 619, 735
BseDI CCNNGG 1 cut(s) 734
BseGI GGATG 4 cut(s) 76, 256, 413, 1049
BseLI CCNNNNNNNGG 3 cut(s) 618, 994, 1261
BseMI GCAATG 1 cut(s) 583
BseMII CTCAG 2 cut(s) 37, 95
BseNI ACTGG 2 cut(s) 386, 1045
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 3 cut(s) 485, 536, 1121
BseYI CCCAGC 2 cut(s) 246, 963
BshFI GGCC 4 cut(s) 10, 412, 439, 708
BshNI GGYRCC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 1255
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 3 cut(s) 618, 994, 1261
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 2 cut(s) 689, 716
BsnI GGCC 4 cut(s) 10, 412, 439, 708
Bsp1286I GDGCHC 1 cut(s) 1237
Bsp1407I TGTACA 1 cut(s) 452
Bsp143I GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
BspACI CCGC 2 cut(s) 405, 1206
BspANI GGCC 4 cut(s) 10, 412, 439, 708
BspCNI CTCAG 2 cut(s) 36, 94
BspHI TCATGA 2 cut(s) 660, 720
BspLI GGNNCC 4 cut(s) 307, 386, 1088, 1089
BspPI GGATC 5 cut(s) 150, 235, 244, 603, 1087
BspQI GCTCTTC 1 cut(s) 913
BspT107I GGYRCC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 583
BsrGI TGTACA 1 cut(s) 452
BsrI ACTGG 2 cut(s) 386, 1045
BssECI CCNNGG 1 cut(s) 734
BssMI GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
BssNAI GTATAC 1 cut(s) 1000
Bst1107I GTATAC 1 cut(s) 1000
Bst2UI CCWGG 2 cut(s) 619, 735
Bst4CI ACNGT 2 cut(s) 46, 389
Bst6I CTCTTC 1 cut(s) 913
BstAFI CTTAAG 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 767
BstAUI TGTACA 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 4 cut(s) 23, 81, 399, 942
BstF5I GGATG 4 cut(s) 76, 256, 413, 1049
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 7 cut(s) 145, 230, 239, 532, 611, 1095, 1125
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
BstMWI GCNNNNNNNGC 5 cut(s) 269, 524, 767, 943, 1055
BstNI CCWGG 2 cut(s) 619, 735
BstNSI RCATGY 2 cut(s) 765, 927
BstSCI CCNGG 3 cut(s) 617, 733, 1254
BstSFI CTRYAG 3 cut(s) 423, 566, 1210
BstSLI GKGCMC 1 cut(s) 1237
BstV1I GCAGC 3 cut(s) 485, 536, 1121
BstV2I GAAGAC 1 cut(s) 1168
BstX2I RGATCY 2 cut(s) 236, 608
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 2 cut(s) 236, 608
BstZ17I GTATAC 1 cut(s) 1000
Bsu36I CCTNAGG 1 cut(s) 23
BsuRI GGCC 4 cut(s) 10, 412, 439, 708
BtsCI GGATG 4 cut(s) 76, 256, 413, 1049
BtsIMutI CAGTG 2 cut(s) 42, 1038
Cac8I GCNNGC 1 cut(s) 441
CciI TCATGA 2 cut(s) 660, 720
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 52, 411, 1087
Csp6I GTAC 5 cut(s) 113, 160, 385, 453, 1265
CviQI GTAC 5 cut(s) 113, 160, 385, 453, 1265
DdeI CTNAG 4 cut(s) 23, 81, 399, 942
DpnI GATC 7 cut(s) 144, 229, 238, 531, 610, 1094, 1124
DpnII GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
EaeI YGGCCR 1 cut(s) 8
Eam1104I CTCTTC 1 cut(s) 913
EarI CTCTTC 1 cut(s) 913
EciI GGCGGA 2 cut(s) 420, 1195
Eco147I AGGCCT 1 cut(s) 708
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 52, 1087
Eco57I CTGAAG 2 cut(s) 429, 480
Eco81I CCTNAGG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 1087
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 617, 733
EcoRV GATATC 1 cut(s) 658
EcoT22I ATGCAT 1 cut(s) 718
FaqI GGGAC 1 cut(s) 1100
FauNDI CATATG 1 cut(s) 595
FbaI TGATCA 2 cut(s) 529, 1122
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 3 cut(s) 474, 525, 1110
FokI GGATG 4 cut(s) 83, 263, 420, 1056
Fsp4HI GCNGC 3 cut(s) 474, 525, 1110
GlaI GCGC 1 cut(s) 324
GluI GCNGC 3 cut(s) 474, 525, 1110
GsaI CCCAGC 2 cut(s) 250, 967
HaeIII GGCC 4 cut(s) 10, 412, 439, 708
HapII CCGG 1 cut(s) 1255
HhaI GCGC 1 cut(s) 325
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HinfI GANTC 3 cut(s) 180, 953, 1173
HpaII CCGG 1 cut(s) 1255
HphI GGTGA 2 cut(s) 31, 1091
Hpy166II GTNNAC 2 cut(s) 1000, 1036
Hpy188I TCNGA 5 cut(s) 147, 154, 448, 744, 900
Hpy188III TCNNGA 6 cut(s) 177, 661, 721, 790, 806, 1010
Hpy8I GTNNAC 2 cut(s) 1000, 1036
HpyAV CCTTC 6 cut(s) 313, 424, 455, 463, 698, 1007
HpyCH4III ACNGT 2 cut(s) 46, 389
HpyCH4IV ACGT 1 cut(s) 490
HpyCH4V TGCA 9 cut(s) 93, 203, 223, 590, 687, 716, 770, 1102, 1191
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 524, 767, 943, 1055
HpyF3I CTNAG 4 cut(s) 23, 81, 399, 942
HpySE526I ACGT 1 cut(s) 490
HspAI GCGC 1 cut(s) 323
KflI GGGWCCC 1 cut(s) 1087
KpnI GGTACC 1 cut(s) 388
Ksp22I TGATCA 2 cut(s) 529, 1122
Kzo9I GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
LguI GCTCTTC 1 cut(s) 913
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 3 cut(s) 485, 536, 1121
LweI GCATC 4 cut(s) 532, 599, 748, 1111
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 3 cut(s) 377, 389, 1199
MalI GATC 7 cut(s) 144, 229, 238, 531, 610, 1094, 1124
MboI GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
MboII GAAGA 4 cut(s) 218, 757, 930, 1168
MflI RGATCY 2 cut(s) 236, 608
MhlI GDGCHC 1 cut(s) 1237
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 4 cut(s) 148, 725, 1062, 1126
MluNI TGGCCA 1 cut(s) 10
MlyI GAGTC 1 cut(s) 1182
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 32, 61, 133, 287, 575
Mox20I TGGCCA 1 cut(s) 10
Mph1103I ATGCAT 1 cut(s) 718
MscI TGGCCA 1 cut(s) 10
MseI TTAA 4 cut(s) 315, 549, 711, 875
MslI CAYNNNNRTG 2 cut(s) 44, 47
Msp20I TGGCCA 1 cut(s) 10
MspA1I CMGCKG 3 cut(s) 458, 527, 641
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 1 cut(s) 1255
MspR9I CCNGG 3 cut(s) 619, 735, 1256
Mva1269I GAATGC 2 cut(s) 689, 716
MvaI CCWGG 2 cut(s) 619, 735
MwoI GCNNNNNNNGC 5 cut(s) 269, 524, 767, 943, 1055
NciI CCSGG 1 cut(s) 1256
NdeI CATATG 1 cut(s) 595
NdeII GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
NlaIV GGNNCC 4 cut(s) 307, 386, 1088, 1089
NmuCI GTSAC 1 cut(s) 389
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 2 cut(s) 765, 927
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 2 cut(s) 660, 720
PceI AGGCCT 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 913
PctI GAATGC 2 cut(s) 689, 716
PfeI GAWTC 2 cut(s) 180, 953
PkrI GCNGC 3 cut(s) 475, 526, 1111
PleI GAGTC 1 cut(s) 1181
PpsI GAGTC 1 cut(s) 1181
Ppu21I YACGTR 1 cut(s) 491
PpuMI RGGWCCY 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 1087
Psp6I CCWGG 2 cut(s) 617, 733
PspFI CCCAGC 2 cut(s) 246, 963
PspGI CCWGG 2 cut(s) 617, 733
PspN4I GGNNCC 4 cut(s) 307, 386, 1088, 1089
PspPI GGNCC 3 cut(s) 52, 411, 1087
PspPPI RGGWCCY 1 cut(s) 1087
PsuI RGATCY 2 cut(s) 236, 608
PvuII CAGCTG 3 cut(s) 458, 527, 641
RsaI GTAC 5 cut(s) 114, 161, 386, 454, 1266
RsaNI GTAC 5 cut(s) 113, 160, 385, 453, 1265
RseI CAYNNNNRTG 2 cut(s) 44, 47
SapI GCTCTTC 1 cut(s) 913
SaqAI TTAA 4 cut(s) 315, 549, 711, 875
SatI GCNGC 3 cut(s) 474, 525, 1110
Sau3AI GATC 7 cut(s) 142, 227, 236, 529, 608, 1092, 1122
Sau96I GGNCC 3 cut(s) 52, 411, 1087
SchI GAGTC 1 cut(s) 1182
ScrFI CCNGG 3 cut(s) 619, 735, 1256
SduI GDGCHC 1 cut(s) 1237
SfaNI GCATC 4 cut(s) 532, 599, 748, 1111
SfcI CTRYAG 3 cut(s) 423, 566, 1210
SinI GGWCC 2 cut(s) 52, 1087
SmiMI CAYNNNNRTG 2 cut(s) 44, 47
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 4 cut(s) 148, 725, 1062, 1126
SseBI AGGCCT 1 cut(s) 708
SsiI CCGC 2 cut(s) 405, 1206
StuI AGGCCT 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 617, 733, 1254
TaaI ACNGT 2 cut(s) 46, 389
TaiI ACGT 1 cut(s) 493
TaqI TCGA 6 cut(s) 230, 789, 951, 1011, 1105, 1249
TasI AATT 4 cut(s) 148, 725, 1062, 1126
TatI WGTACW 1 cut(s) 452
TfiI GAWTC 2 cut(s) 180, 953
Tru1I TTAA 4 cut(s) 315, 549, 711, 875
Tru9I TTAA 4 cut(s) 315, 549, 711, 875
TscAI CASTG 2 cut(s) 49, 1045
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 473, 524, 1109
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 8 cut(s) 87, 230, 312, 322, 612, 709, 737, 775
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 2 cut(s) 49, 1045
Vha464I CTTAAG 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 52, 1087
XapI RAATTY 1 cut(s) 148
XceI RCATGY 2 cut(s) 765, 927
XmiI GTMKAC 1 cut(s) 999
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.