Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Reverse (-)

52761739 .. 52774650

Gene Structure 12912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009434

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAAGAGATGGTAATCCATGGCTCACAAGTGTGAATAATCACATCGGGAGACAATTCTGGGAGTTCGACCCGAATCTCGAAACACCGGAAGAGCGAGCTCAAGTGGAAAAAGCACGTTTTGAGTTTCATAACAATCGTTTTCGATCCAAACATAGTTCCGATCTATTAATGAGACTTCAGTTTGCAAAGGAGAAAGAAAGTGATCGAATGGAGCTACCGGCTCAGGTGAAAATAAAAAGTGAAGAAGAAATTAATGAAGAAGTAGTAACAACCACATTGAGGAGGGGGTTGAGATTTTACTCGACTCTTCAGGCTGAGGATGGTTTCTGGCCTGGTGATTATGGCGGTCCTTTATTTCTTCTACCTAGCTTGGTTATTGGATTATCTGTAACAGGGGCCCTTGAGGCAATCTTGCCACACGAGTATTCAAAGGAGATCCGCCGCTATCTTTATAACCATCAGAACAAAGATGGAGGTTGGGGACTGCATATAGAAGGGCCAAGTACAATGTTTTGTACAGCTCTGTCCTATGTGAGTCTGAGATTGCTCGGAGAGGAAATGGACGGCGGTGATGGCGCCATGCAGAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCACCATCCCATCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATAAATGGGACGGTAACAATCCCCTGCCTCCTGAACTGTGGCTTTTACCGTACTTTCTCCCAGTACACCCAGGCCGAATGTGGTGTCACTGTAGGATGGTATACCTGCCCATGTCATATTTATACGGCAAGAGATTTGTTGGTCCCATCAACGGCCTTATTTTATCCCTCAGAACAGAGCTTTATACCTTTCCCTATCACCTGATTGATTGGGACCAAGCAAGAAATCTATGCGCCAAGGAGGATTTGTATTACCCACATCCCATTTTACAGGACGTGCTGTGGGGGTGTCTTCATAAGGTTGGGGAACCTCTTCTCATGAGATGGCCATTGTCTAACCTCAGGCAAAAAGCTCTCACCACTGTGATGGACCACATACACTATGAGGATGAAAACACTCAGTATATCTGCCTTGGATCTGTCAATAAGGTACTAAACATGATTTGCTGTTGGGTGGAGGATCAGAATTCAGAAGCATACAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATCGAATGGATCTCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTCTCTACCAACCTTGTGAATGAATATGGTTCCGCGCTTAAGAAGGCGCACTATTTCATCAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTGCGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCTAGACAATGGTTGGCCTGCTTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTACTATCACGTATGCCCTGCGAAATTGTGGGAGAAGCAATAGCAGCTGATCAACTGCTTGATGCTGTTAATGTAGTATTATCCCTACAGAACAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCATGACTGATTATCAGTATGTGGAATGCACATCATCAGCAGTTGAAGGCCTTAATGCATTCATGAAATTATATCCAGGGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCAAGAGCATACAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTGGTGGCAAGACTTATCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTCTCATGCCAAAACAAGGTATACACAAATCTCGAAGGGAACAAACCACATATAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATAAATTCACAGATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAGCAAAAATTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTGGAGAGTATCGAAACCGGGTATTGTGTATTAGTGGTTGTGCAAATTTGATCGTGTTTATCAAGCCCATTTTCAGGAATAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

779

Amino Acids

88.76

Weight (kDa)

7.51

Isoelectric Point (pI)

44.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	145 - 365	1.6e-36	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	418 - 756	1.7e-53	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	480
Acc36I	ACCTGC	1 cut(s)	810
Acc65I	GGTACC	1 cut(s)	1398
AccB1I	GGYRCC	2 cut(s)	602, 1398
AccI	GTMKAC	2 cut(s)	798, 2013
AccII	CGCG	1 cut(s)	1325
AciI	CCGC	7 cut(s)	372, 466, 469, 594, 1323, 1419, 2220
AclWI	GGATC	8 cut(s)	165, 457, 1120, 1164, 1249, 1258, 1617, 2101
AcoI	YGGCCR	1 cut(s)	1022
AcsI	RAATTY	3 cut(s)	1162, 2140, 2296
AcuI	CTGAAG	6 cut(s)	32, 188, 320, 1443, 1494, 2176
AcyI	GRCGYC	1 cut(s)	603
AfaI	GTAC	8 cut(s)	532, 544, 687, 749, 762, 1128, 1400, 1468
AfiI	CCNNNNNNNGG	7 cut(s)	306, 664, 665, 848, 1632, 2008, 2325
AflII	CTTAAG	1 cut(s)	1328
AgsI	TTSAA	6 cut(s)	456, 1191, 1283, 1483, 1718, 2081
AjiI	CACGTC	1 cut(s)	973
AjnI	CCWGG	4 cut(s)	358, 766, 1631, 1747
AleI	CACNNNNGTG	2 cut(s)	56, 1058
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	3 cut(s)	70, 193, 1365
AlwI	GGATC	8 cut(s)	165, 457, 1120, 1164, 1249, 1258, 1617, 2101
AoxI	GGCC	9 cut(s)	356, 423, 524, 769, 850, 1022, 1424, 1451, 1720
ApaI	GGGCCC	1 cut(s)	427
ApeKI	GCWGC	3 cut(s)	1487, 1538, 2123
ApoI	RAATTY	3 cut(s)	1162, 2140, 2296
ArsI	GACNNNNNNTTYG	2 cut(s)	766, 798
AseI	ATTAAT	2 cut(s)	194, 279
Asp700I	GAANNNNTTC	1 cut(s)	1008
Asp718I	GGTACC	1 cut(s)	1398
AspLEI	GCGC	4 cut(s)	605, 932, 1327, 1339
AspS9I	GGNCC	9 cut(s)	374, 423, 424, 524, 839, 910, 1066, 1425, 2101
AsuC2I	CCSGG	1 cut(s)	2270
AsuHPI	GGTGA	7 cut(s)	265, 374, 608, 626, 887, 1045, 2105
AvaII	GGWCC	5 cut(s)	374, 839, 910, 1066, 2101
AxyI	CCTNAGG	1 cut(s)	1037
BaeGI	GKGCMC	2 cut(s)	427, 2251
BalI	TGGCCA	1 cut(s)	1024
BanI	GGYRCC	2 cut(s)	602, 1398
BanII	GRGCYC	2 cut(s)	127, 427
BauI	CACGAG	1 cut(s)	446
BbsI	GAAGAC	2 cut(s)	980, 2182
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	342
BbvI	GCAGC	3 cut(s)	1499, 1550, 2135
BceAI	ACGGC	3 cut(s)	607, 838, 865
BcgI	CGANNNNNNTGC	2 cut(s)	2095, 2129
BciT130I	CCWGG	4 cut(s)	360, 768, 1633, 1749
BclI	TGATCA	2 cut(s)	1543, 1644
BcnI	CCSGG	1 cut(s)	2270
BcoDI	GTCTC	3 cut(s)	70, 193, 1365
BfaI	CTAG	2 cut(s)	393, 1439
BfmI	CTRYAG	3 cut(s)	787, 1580, 2224
BfoI	RGCGCY	1 cut(s)	606
BfrI	CTTAAG	1 cut(s)	1328
BfuAI	ACCTGC	1 cut(s)	810
BglI	GCCNNNNNGGC	1 cut(s)	431
BisI	GCNGC	4 cut(s)	469, 1488, 1539, 2124
BlsI	GCNGC	4 cut(s)	470, 1489, 1540, 2125
BmcAI	AGTACT	1 cut(s)	687
Bme1390I	CCNGG	5 cut(s)	360, 768, 1633, 1749, 2270
Bme18I	GGWCC	5 cut(s)	374, 839, 910, 1066, 2101
BmgBI	CACGTC	1 cut(s)	973
BmgT120I	GGNCC	9 cut(s)	374, 423, 424, 524, 839, 910, 1066, 1425, 2101
BmrFI	CCNGG	5 cut(s)	360, 768, 1633, 1749, 2270
BmrI	ACTGGG	1 cut(s)	752
BmsI	GCATC	4 cut(s)	1546, 1613, 1762, 2125
BmuI	ACTGGG	1 cut(s)	752
BpiI	GAAGAC	2 cut(s)	980, 2182
Bpu10I	CCTNAGC	2 cut(s)	249, 342
BpuEI	CTTGAG	2 cut(s)	111, 449
BpuMI	CCSGG	1 cut(s)	2270
BsaAI	YACGTR	1 cut(s)	1505
BsaBI	GATNNNNATC	1 cut(s)	39
BsaHI	GRCGYC	1 cut(s)	603
BsaJI	CCNNGG	6 cut(s)	44, 637, 766, 933, 1108, 1748
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	2 cut(s)	929, 959
Bsc4I	CCNNNNNNNGG	7 cut(s)	306, 664, 665, 848, 1632, 2008, 2325
Bse118I	RCCGGY	1 cut(s)	244
Bse1I	ACTGG	3 cut(s)	758, 1400, 2059
Bse21I	CCTNAGG	1 cut(s)	1037
Bse3DI	GCAATG	1 cut(s)	1597
Bse8I	GATNNNNATC	1 cut(s)	39
BseBI	CCWGG	4 cut(s)	360, 768, 1633, 1749
BseDI	CCNNGG	6 cut(s)	44, 637, 766, 933, 1108, 1748
BseGI	GGATG	8 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 2063
BseJI	GATNNNNATC	1 cut(s)	39
BseLI	CCNNNNNNNGG	7 cut(s)	306, 664, 665, 848, 1632, 2008, 2325
BseMI	GCAATG	1 cut(s)	1597
BseMII	CTCAG	7 cut(s)	263, 333, 557, 696, 880, 1051, 1109
BseNI	ACTGG	3 cut(s)	758, 1400, 2059
BseRI	GAGGAG	2 cut(s)	322, 657
BseSI	GKGCMC	2 cut(s)	427, 2251
BseXI	GCAGC	3 cut(s)	1499, 1550, 2135
BseYI	CCCAGC	2 cut(s)	1260, 1977
Bsh1236I	CGCG	1 cut(s)	1325
BshFI	GGCC	9 cut(s)	358, 425, 526, 771, 852, 1024, 1426, 1453, 1722
BshNI	GGYRCC	2 cut(s)	602, 1398
BsiHKAI	GWGCWC	1 cut(s)	127
BsiSI	CCGG	3 cut(s)	113, 245, 2269
BslFI	GGGAC	5 cut(s)	522, 719, 825, 923, 2114
BslI	CCNNNNNNNGG	7 cut(s)	306, 664, 665, 848, 1632, 2008, 2325
BsmAI	GTCTC	3 cut(s)	70, 193, 1365
BsmFI	GGGAC	5 cut(s)	522, 719, 825, 923, 2114
BsmI	GAATGC	2 cut(s)	1703, 1730
BsnI	GGCC	9 cut(s)	358, 425, 526, 771, 852, 1024, 1426, 1453, 1722
Bsp120I	GGGCCC	1 cut(s)	423
Bsp1286I	GDGCHC	3 cut(s)	127, 427, 2251
Bsp1407I	TGTACA	2 cut(s)	542, 1466
Bsp19I	CCATGG	1 cut(s)	44
BspACI	CCGC	7 cut(s)	372, 466, 469, 594, 1323, 1419, 2220
BspANI	GGCC	9 cut(s)	358, 425, 526, 771, 852, 1024, 1426, 1453, 1722
BspCNI	CTCAG	7 cut(s)	262, 334, 558, 695, 879, 1050, 1108
BspFNI	CGCG	1 cut(s)	1325
BspHI	TCATGA	3 cut(s)	1014, 1674, 1734
BspMI	ACCTGC	1 cut(s)	810
BspPI	GGATC	8 cut(s)	165, 457, 1120, 1164, 1249, 1258, 1617, 2101
BspQI	GCTCTTC	2 cut(s)	111, 1927
BspT107I	GGYRCC	2 cut(s)	602, 1398
BspTI	CTTAAG	1 cut(s)	1328
BsrDI	GCAATG	1 cut(s)	1597
BsrFI	RCCGGY	1 cut(s)	244
BsrGI	TGTACA	2 cut(s)	542, 1466
BsrI	ACTGG	3 cut(s)	758, 1400, 2059
BssAI	RCCGGY	1 cut(s)	244
BssECI	CCNNGG	6 cut(s)	44, 637, 766, 933, 1108, 1748
BssNAI	GTATAC	2 cut(s)	799, 2014
BssNI	GRCGYC	1 cut(s)	603
BssSI	CACGAG	1 cut(s)	446
BssT1I	CCWWGG	3 cut(s)	44, 933, 1108
Bst1107I	GTATAC	2 cut(s)	799, 2014
Bst2BI	CACGAG	1 cut(s)	446
Bst2UI	CCWGG	4 cut(s)	360, 768, 1633, 1749
Bst4CI	ACNGT	7 cut(s)	638, 710, 735, 747, 788, 1060, 1403
Bst6I	CTCTTC	5 cut(s)	25, 111, 339, 1014, 1927
BstACI	GRCGYC	1 cut(s)	603
BstAFI	CTTAAG	1 cut(s)	1328
BstAPI	GCANNNNNTGC	2 cut(s)	1781, 2202
BstAUI	TGTACA	2 cut(s)	542, 1466
BstBAI	YACGTR	1 cut(s)	1505
BstC8I	GCNNGC	2 cut(s)	123, 1455
BstDEI	CTNAG	9 cut(s)	249, 342, 566, 682, 866, 1037, 1095, 1413, 1956
BstDSI	CCRYGG	2 cut(s)	44, 637
BstF5I	GGATG	8 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 2063
BstFNI	CGCG	1 cut(s)	1325
BstH2I	RGCGCY	1 cut(s)	606
BstHHI	GCGC	4 cut(s)	605, 932, 1327, 1339
BstMAI	GTCTC	3 cut(s)	70, 193, 1365
BstMWI	GCNNNNNNNGC	8 cut(s)	431, 600, 1283, 1538, 1781, 1957, 2069, 2202
BstNI	CCWGG	4 cut(s)	360, 768, 1633, 1749
BstNSI	RCATGY	2 cut(s)	1779, 1941
BstSCI	CCNGG	5 cut(s)	358, 766, 1631, 1747, 2268
BstSFI	CTRYAG	3 cut(s)	787, 1580, 2224
BstSLI	GKGCMC	2 cut(s)	427, 2251
BstUI	CGCG	1 cut(s)	1325
BstV1I	GCAGC	3 cut(s)	1499, 1550, 2135
BstV2I	GAAGAC	2 cut(s)	980, 2182
BstX2I	RGATCY	4 cut(s)	462, 1112, 1250, 1622
BstXI	CCANNNNNNTGG	1 cut(s)	1063
BstYI	RGATCY	4 cut(s)	462, 1112, 1250, 1622
BstZ17I	GTATAC	2 cut(s)	799, 2014
Bsu36I	CCTNAGG	1 cut(s)	1037
BsuRI	GGCC	9 cut(s)	358, 425, 526, 771, 852, 1024, 1426, 1453, 1722
BtgI	CCRYGG	2 cut(s)	44, 637
BtrI	CACGTC	1 cut(s)	973
BtsCI	GGATG	8 cut(s)	352, 654, 798, 955, 1090, 1270, 1427, 2063
BtsIMutI	CAGTG	3 cut(s)	784, 1056, 2052
BveI	ACCTGC	1 cut(s)	810
Cac8I	GCNNGC	2 cut(s)	123, 1455
CciI	TCATGA	3 cut(s)	1014, 1674, 1734
CfoI	GCGC	4 cut(s)	605, 932, 1327, 1339
Cfr10I	RCCGGY	1 cut(s)	244
Cfr13I	GGNCC	9 cut(s)	374, 423, 424, 524, 839, 910, 1066, 1425, 2101
Csp6I	GTAC	8 cut(s)	531, 543, 686, 748, 761, 1127, 1399, 1467
CviQI	GTAC	8 cut(s)	531, 543, 686, 748, 761, 1127, 1399, 1467
DdeI	CTNAG	9 cut(s)	249, 342, 566, 682, 866, 1037, 1095, 1413, 1956
DinI	GGCGCC	1 cut(s)	604
EaeI	YGGCCR	1 cut(s)	1022
Eam1104I	CTCTTC	5 cut(s)	25, 111, 339, 1014, 1927
EarI	CTCTTC	5 cut(s)	25, 111, 339, 1014, 1927
EciI	GGCGGA	3 cut(s)	455, 1434, 2209
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	3 cut(s)	44, 933, 1108
Eco147I	AGGCCT	1 cut(s)	1722
Eco24I	GRGCYC	2 cut(s)	127, 427
Eco32I	GATATC	1 cut(s)	1672
Eco47I	GGWCC	5 cut(s)	374, 839, 910, 1066, 2101
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	6 cut(s)	32, 188, 320, 1443, 1494, 2176
Eco81I	CCTNAGG	1 cut(s)	1037
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	3 cut(s)	423, 424, 2101
EcoRI	GAATTC	1 cut(s)	1162
EcoRII	CCWGG	4 cut(s)	358, 766, 1631, 1747
EcoRV	GATATC	1 cut(s)	1672
EcoT14I	CCWWGG	3 cut(s)	44, 933, 1108
EcoT22I	ATGCAT	1 cut(s)	1732
EcoT38I	GRGCYC	2 cut(s)	127, 427
EgeI	GGCGCC	1 cut(s)	604
EheI	GGCGCC	1 cut(s)	604
ErhI	CCWWGG	3 cut(s)	44, 933, 1108
FaqI	GGGAC	5 cut(s)	522, 719, 825, 923, 2114
FauNDI	CATATG	1 cut(s)	1609
FbaI	TGATCA	2 cut(s)	1543, 1644
FblI	GTMKAC	2 cut(s)	798, 2013
Fnu4HI	GCNGC	4 cut(s)	469, 1488, 1539, 2124
FokI	GGATG	8 cut(s)	359, 641, 805, 942, 1097, 1277, 1434, 2070
FriOI	GRGCYC	2 cut(s)	127, 427
Fsp4HI	GCNGC	4 cut(s)	469, 1488, 1539, 2124
FspBI	CTAG	2 cut(s)	393, 1439
GlaI	GCGC	4 cut(s)	604, 931, 1326, 1338
GluI	GCNGC	4 cut(s)	469, 1488, 1539, 2124
GsaI	CCCAGC	2 cut(s)	1264, 1981
HaeII	RGCGCY	1 cut(s)	606
HaeIII	GGCC	9 cut(s)	358, 425, 526, 771, 852, 1024, 1426, 1453, 1722
HapII	CCGG	3 cut(s)	113, 245, 2269
HhaI	GCGC	4 cut(s)	605, 932, 1327, 1339
Hin1I	GRCGYC	1 cut(s)	603
Hin6I	GCGC	4 cut(s)	603, 930, 1325, 1337
HinP1I	GCGC	4 cut(s)	603, 930, 1325, 1337
HinfI	GANTC	7 cut(s)	100, 331, 562, 628, 1194, 1967, 2187
HpaII	CCGG	3 cut(s)	113, 245, 2269
HphI	GGTGA	7 cut(s)	265, 374, 608, 626, 887, 1045, 2105
Hpy166II	GTNNAC	4 cut(s)	763, 799, 2014, 2050
Hpy8I	GTNNAC	4 cut(s)	763, 799, 2014, 2050
HpyCH4III	ACNGT	7 cut(s)	638, 710, 735, 747, 788, 1060, 1403
HpyCH4IV	ACGT	3 cut(s)	142, 972, 1504
HpyF10VI	GCNNNNNNNGC	8 cut(s)	431, 600, 1283, 1538, 1781, 1957, 2069, 2202
HpyF3I	CTNAG	9 cut(s)	249, 342, 566, 682, 866, 1037, 1095, 1413, 1956
HpySE526I	ACGT	3 cut(s)	142, 972, 1504
Hsp92I	GRCGYC	1 cut(s)	603
HspAI	GCGC	4 cut(s)	603, 930, 1325, 1337
KasI	GGCGCC	1 cut(s)	602
KflI	GGGWCCC	1 cut(s)	2101
KpnI	GGTACC	1 cut(s)	1402
Ksp22I	TGATCA	2 cut(s)	1543, 1644
LguI	GCTCTTC	2 cut(s)	111, 1927
LmnI	GCTCC	1 cut(s)	238
Lsp1109I	GCAGC	3 cut(s)	1499, 1550, 2135
LweI	GCATC	4 cut(s)	1546, 1613, 1762, 2125
MaeI	CTAG	2 cut(s)	393, 1439
MaeII	ACGT	3 cut(s)	142, 972, 1504
MaeIII	GTNAC	8 cut(s)	292, 415, 646, 710, 782, 1391, 1403, 2213
MflI	RGATCY	4 cut(s)	462, 1112, 1250, 1622
MhlI	GDGCHC	3 cut(s)	127, 427, 2251
MlsI	TGGCCA	1 cut(s)	1024
MluCI	AATT	9 cut(s)	80, 276, 1162, 1518, 1739, 2076, 2140, 2200, 2296
MluNI	TGGCCA	1 cut(s)	1024
Mly113I	GGCGCC	1 cut(s)	603
MlyI	GAGTC	3 cut(s)	325, 571, 2196
MmeI	TCCRAC	1 cut(s)	1617
Mox20I	TGGCCA	1 cut(s)	1024
Mph1103I	ATGCAT	1 cut(s)	1732
MroXI	GAANNNNTTC	1 cut(s)	1008
MscI	TGGCCA	1 cut(s)	1024
MseI	TTAA	6 cut(s)	194, 279, 1329, 1563, 1725, 1889
MslI	CAYNNNNRTG	4 cut(s)	56, 1058, 1061, 1177
Msp20I	TGGCCA	1 cut(s)	1024
MspA1I	CMGCKG	3 cut(s)	1472, 1541, 1655
MspCI	CTTAAG	1 cut(s)	1328
MspI	CCGG	3 cut(s)	113, 245, 2269
MspR9I	CCNGG	5 cut(s)	360, 768, 1633, 1749, 2270
Mva1269I	GAATGC	2 cut(s)	1703, 1730
MvaI	CCWGG	4 cut(s)	360, 768, 1633, 1749
MvnI	CGCG	1 cut(s)	1325
MwoI	GCNNNNNNNGC	8 cut(s)	431, 600, 1283, 1538, 1781, 1957, 2069, 2202
NarI	GGCGCC	1 cut(s)	603
NciI	CCSGG	1 cut(s)	2270
NcoI	CCATGG	1 cut(s)	44
NdeI	CATATG	1 cut(s)	1609
NmuCI	GTSAC	2 cut(s)	782, 1403
NsiI	ATGCAT	1 cut(s)	1732
NspI	RCATGY	2 cut(s)	1779, 1941
OliI	CACNNNNGTG	2 cut(s)	56, 1058
PagI	TCATGA	3 cut(s)	1014, 1674, 1734
PceI	AGGCCT	1 cut(s)	1722
PciSI	GCTCTTC	2 cut(s)	111, 1927
PctI	GAATGC	2 cut(s)	1703, 1730
PdmI	GAANNNNTTC	1 cut(s)	1008
PfeI	GAWTC	4 cut(s)	100, 628, 1194, 1967
PkrI	GCNGC	4 cut(s)	470, 1489, 1540, 2125
PleI	GAGTC	3 cut(s)	325, 570, 2195
PluTI	GGCGCC	1 cut(s)	606
PpsI	GAGTC	3 cut(s)	325, 570, 2195
Ppu21I	YACGTR	1 cut(s)	1505
PpuMI	RGGWCCY	1 cut(s)	2101
PshBI	ATTAAT	2 cut(s)	194, 279
PsiI	TTATAA	1 cut(s)	480
Psp124BI	GAGCTC	1 cut(s)	127
Psp5II	RGGWCCY	1 cut(s)	2101
Psp6I	CCWGG	4 cut(s)	358, 766, 1631, 1747
PspFI	CCCAGC	2 cut(s)	1260, 1977
PspGI	CCWGG	4 cut(s)	358, 766, 1631, 1747
PspOMI	GGGCCC	1 cut(s)	423
PspPI	GGNCC	9 cut(s)	374, 423, 424, 524, 839, 910, 1066, 1425, 2101
PspPPI	RGGWCCY	1 cut(s)	2101
PsuI	RGATCY	4 cut(s)	462, 1112, 1250, 1622
PvuII	CAGCTG	3 cut(s)	1472, 1541, 1655
RsaI	GTAC	8 cut(s)	532, 544, 687, 749, 762, 1128, 1400, 1468
RsaNI	GTAC	8 cut(s)	531, 543, 686, 748, 761, 1127, 1399, 1467
RseI	CAYNNNNRTG	4 cut(s)	56, 1058, 1061, 1177
SacI	GAGCTC	1 cut(s)	127
SapI	GCTCTTC	2 cut(s)	111, 1927
SaqAI	TTAA	6 cut(s)	194, 279, 1329, 1563, 1725, 1889
SatI	GCNGC	4 cut(s)	469, 1488, 1539, 2124
Sau96I	GGNCC	9 cut(s)	374, 423, 424, 524, 839, 910, 1066, 1425, 2101
ScaI	AGTACT	1 cut(s)	687
SchI	GAGTC	3 cut(s)	325, 571, 2196
ScrFI	CCNGG	5 cut(s)	360, 768, 1633, 1749, 2270
SduI	GDGCHC	3 cut(s)	127, 427, 2251
SfaNI	GCATC	4 cut(s)	1546, 1613, 1762, 2125
SfcI	CTRYAG	3 cut(s)	787, 1580, 2224
SfoI	GGCGCC	1 cut(s)	604
SinI	GGWCC	5 cut(s)	374, 839, 910, 1066, 2101
SmiMI	CAYNNNNRTG	4 cut(s)	56, 1058, 1061, 1177
SmlI	CTYRAG	3 cut(s)	126, 428, 1328
SmoI	CTYRAG	3 cut(s)	126, 428, 1328
Sse9I	AATT	9 cut(s)	80, 276, 1162, 1518, 1739, 2076, 2140, 2200, 2296
SseBI	AGGCCT	1 cut(s)	1722
SsiI	CCGC	7 cut(s)	372, 466, 469, 594, 1323, 1419, 2220
SspDI	GGCGCC	1 cut(s)	602
SspMI	CTAG	2 cut(s)	393, 1439
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	1 cut(s)	1722
StyD4I	CCNGG	5 cut(s)	358, 766, 1631, 1747, 2268
StyI	CCWWGG	3 cut(s)	44, 933, 1108
TaaI	ACNGT	7 cut(s)	638, 710, 735, 747, 788, 1060, 1403
TaiI	ACGT	3 cut(s)	145, 975, 1507
TasI	AATT	9 cut(s)	80, 276, 1162, 1518, 1739, 2076, 2140, 2200, 2296
TatI	WGTACW	5 cut(s)	530, 542, 685, 760, 1466
TauI	GCSGC	1 cut(s)	471
TfiI	GAWTC	4 cut(s)	100, 628, 1194, 1967
Tru1I	TTAA	6 cut(s)	194, 279, 1329, 1563, 1725, 1889
Tru9I	TTAA	6 cut(s)	194, 279, 1329, 1563, 1725, 1889
TscAI	CASTG	3 cut(s)	791, 1063, 2059
TseFI	GTSAC	2 cut(s)	782, 1403
TseI	GCWGC	3 cut(s)	1487, 1538, 2123
Tsp45I	GTSAC	2 cut(s)	782, 1403
TspRI	CASTG	3 cut(s)	791, 1063, 2059
Vha464I	CTTAAG	1 cut(s)	1328
VpaK11BI	GGWCC	5 cut(s)	374, 839, 910, 1066, 2101
VspI	ATTAAT	2 cut(s)	194, 279
XapI	RAATTY	3 cut(s)	1162, 2140, 2296
XbaI	TCTAGA	1 cut(s)	1438
XceI	RCATGY	2 cut(s)	1779, 1941
XcmI	CCANNNNNNNNNTGG	1 cut(s)	774
XmiI	GTMKAC	2 cut(s)	798, 2013
XmnI	GAANNNNTTC	1 cut(s)	1008
XspI	CTAG	2 cut(s)	393, 1439
ZrmI	AGTACT	1 cut(s)	687
Zsp2I	ATGCAT	1 cut(s)	1732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.