Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

13592801 .. 13604075

Gene Structure 11275 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ36957

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGTGGAGGCTGAAGGTTTCAGAAGGAGGAAGAGATGGTAACCCATGGCTCACAAGTGTGAATAATCACGTCGGGAGACAATTTTGGGAGTTTGACCCAAATCTCGGAACACCGGAAGAGCGAGCTCAGGTGGAAAAAACACGTCTTGAGTTTCGTAATAATCGTTTTCAATCCAAACATAGTTCTGATCTATTAATGAGACTTCAGAAAGAAAGTGATCGAATGGAGCTACCAGCTCAGGTGAAAATAAAAAGCGAAGAAGAAATTAATGAAGAAGTAGTAGCAACCACATTGAGGAGGGGGTTGAGATTTTACTCGACTCTTCAGGCTGAGGATGGTTTCTGGCCTGGTGACTATGGCGGTCCTTTGTTTCTTCTACCTGGCTTGGTTATTGGATTATCCGTAACAGGGGCCCTTGATGCAATCTTGCCACACGAGCATTCAAAGGAGATCCGCCGCTATCTTTATAACCATCAGAACAAAGATGGAGGTTGGGGACTGCATATAGAAGGGCCAAGTACAATGTTTTGTACAGCTCTGTCCTACGTGAGTTTGAGATTGCTCGGAGAGGAGATGGATGGCGGTGAGGACGCCATGGACAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCACCATCCCGTCATGGGGAAAGATGTGGCTCTCAGTACTTGGTGTATATGAATGGGATGGTAACAATCCACTGCCTCCTGAATTGTGGCTTTTACCTTACTTTCTCCCAGTACACCCAGGTCGAATGTGGTGTCACTGCAGGATGGTATACCTGCCCATGTCATATTTATACGGCAAGAGATTTGTTGGTCCCATCAACGGCCTTATTTTATCCCTCAGAACAGAGCTTTATACCTTTCCCTATCACCTGATTGATTGGGACCAAGCAAGAAATCTATGCGCAAAGATGATGAACCATTTCAAATTTGTAAGTATGCCCTGGGATTTTGAAGAGCAAAGGCTTACGATGATAAAAAACATGCAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCATATAAGTGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCCCAATGCTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTTTCTACCAACCTTGTGAATGAATATGGTTCTGTGCTTAAGAAGGCGCACTATTTCATCAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTTAGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCCAGACAATGGTTGGCCTGTCTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTATTATCACATATGCCCTGTGAAATTGTGGGAGAAGCAATATCAGCTGATCAACTTCATGATGCTGTTAATGTAGTATTATCCCTACAGGTACAACTTGACCTTGTGACGTCTTATAGTACAATTACAAAGTATTTATGTAATCGCAAGAGCAATGACATGTATTTTATTTCTTTGACTCAACAGAACAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCCTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATATCGTTATTGATTATCAGTACGTGGAATGCACATCAGCAGCAGTTGAAGGCCTTAAAGCATTCATGAAATTATATCCAGGGCATCGGAAGAAAGAAATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATCCAACTTCCTGATGGCTCATGGTATGGGTCTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTAGTGGCAAGACCTATCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTGTCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTTTCATGCCAAAACAAGGTATACACAAATCTCGAAGGGAACAAACCACATGTAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAGATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAAAAATTGCATGATTAGTTACTCGGGCTACAGAAATATTTTTCCTATTTGGGCACTTGGAGAGTATCGAAACCGGGTGCTGGTACTTCACAAATGTATCAATCAAAGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

760

Amino Acids

86.96

Weight (kDa)

7.05

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	143 - 299	2.3e-35	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	516 - 747	7.1e-42	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	468
AatII	GACGTC	1 cut(s)	1484
Acc16I	TGCGCA	1 cut(s)	919
Acc36I	ACCTGC	2 cut(s)	798, 991
Acc65I	GGTACC	1 cut(s)	1275
AccB1I	GGYRCC	1 cut(s)	1275
AccB7I	CCANNNNNTGG	1 cut(s)	1322
AccI	GTMKAC	2 cut(s)	786, 1986
AciI	CCGC	5 cut(s)	360, 454, 457, 582, 1296
AclWI	GGATC	6 cut(s)	445, 1041, 1122, 1135, 1590, 2074
AcsI	RAATTY	2 cut(s)	941, 1039
AcuI	CTGAAG	4 cut(s)	32, 188, 308, 1371
AcyI	GRCGYC	2 cut(s)	591, 1481
AflII	CTTAAG	1 cut(s)	1205
AflIII	ACRYGT	3 cut(s)	140, 1530, 2014
AjiI	CACGTC	2 cut(s)	70, 143
AjnI	CCWGG	6 cut(s)	346, 379, 754, 956, 1604, 1720
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	4 cut(s)	70, 193, 1242, 1339
AlwI	GGATC	6 cut(s)	445, 1041, 1122, 1135, 1590, 2074
Ama87I	CYCGRG	1 cut(s)	2191
AoxI	GGCC	7 cut(s)	344, 411, 512, 838, 1301, 1328, 1693
ApaI	GGGCCC	1 cut(s)	415
ApeKI	GCWGC	3 cut(s)	1364, 1682, 2096
ApoI	RAATTY	2 cut(s)	941, 1039
ArsI	GACNNNNNNTTYG	2 cut(s)	754, 786
AseI	ATTAAT	2 cut(s)	194, 267
Asp700I	GAANNNNTTC	1 cut(s)	935
Asp718I	GGTACC	1 cut(s)	1275
AspLEI	GCGC	2 cut(s)	920, 1216
AspS9I	GGNCC	8 cut(s)	362, 411, 412, 512, 827, 898, 1302, 2074
AsuC2I	CCSGG	1 cut(s)	2243
AsuHPI	GGTGA	6 cut(s)	253, 362, 596, 614, 875, 2078
AvaI	CYCGRG	1 cut(s)	2191
AvaII	GGWCC	4 cut(s)	362, 827, 898, 2074
BaeGI	GKGCMC	2 cut(s)	415, 2224
BaeI	ACNNNNGTAYC	2 cut(s)	2248, 2281
BamHI	GGATCC	1 cut(s)	1127
BanI	GGYRCC	1 cut(s)	1275
BanII	GRGCYC	2 cut(s)	127, 415
BauI	CACGAG	1 cut(s)	434
BbsI	GAAGAC	1 cut(s)	2155
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	330
BbvI	GCAGC	3 cut(s)	1376, 1694, 2108
BceAI	ACGGC	2 cut(s)	826, 853
BcgI	CGANNNNNNTGC	2 cut(s)	2068, 2102
BciT130I	CCWGG	6 cut(s)	348, 381, 756, 958, 1606, 1722
BclI	TGATCA	3 cut(s)	1420, 1617, 2109
BcnI	CCSGG	1 cut(s)	2243
BcoDI	GTCTC	4 cut(s)	70, 193, 1242, 1339
BfaI	CTAG	1 cut(s)	1869
BfmI	CTRYAG	3 cut(s)	775, 1457, 2197
BfrI	CTTAAG	1 cut(s)	1205
BfuAI	ACCTGC	2 cut(s)	798, 991
BisI	GCNGC	4 cut(s)	457, 1365, 1683, 2097
BlsI	GCNGC	4 cut(s)	458, 1366, 1684, 2098
BmcAI	AGTACT	1 cut(s)	675
Bme1390I	CCNGG	7 cut(s)	348, 381, 756, 958, 1606, 1722, 2243
Bme18I	GGWCC	4 cut(s)	362, 827, 898, 2074
BmeT110I	CYCGRG	1 cut(s)	2191
BmgBI	CACGTC	2 cut(s)	70, 143
BmgT120I	GGNCC	8 cut(s)	362, 411, 412, 512, 827, 898, 1302, 2074
BmiI	GGNNCC	8 cut(s)	412, 413, 829, 899, 1129, 1277, 2075, 2076
BmrFI	CCNGG	7 cut(s)	348, 381, 756, 958, 1606, 1722, 2243
BmrI	ACTGGG	1 cut(s)	740
BmsI	GCATC	6 cut(s)	409, 1423, 1586, 1735, 1791, 2098
BmuI	ACTGGG	1 cut(s)	740
BpiI	GAAGAC	1 cut(s)	2155
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	3 cut(s)	126, 237, 330
BpuEI	CTTGAG	1 cut(s)	167
BpuMI	CCSGG	1 cut(s)	2243
BsaAI	YACGTR	2 cut(s)	547, 1666
BsaHI	GRCGYC	2 cut(s)	591, 1481
BsaJI	CCNNGG	7 cut(s)	44, 594, 625, 754, 956, 957, 1721
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	2 cut(s)	1632, 1662
Bse1I	ACTGG	3 cut(s)	746, 1277, 2032
Bse3DI	GCAATG	2 cut(s)	1531, 1570
BseBI	CCWGG	6 cut(s)	348, 381, 756, 958, 1606, 1722
BseDI	CCNNGG	7 cut(s)	44, 594, 625, 754, 956, 957, 1721
BseGI	GGATG	9 cut(s)	340, 583, 642, 700, 786, 1147, 1304, 1782, 2036
BseMI	GCAATG	2 cut(s)	1531, 1570
BseMII	CTCAG	6 cut(s)	140, 251, 321, 684, 868, 1350
BseNI	ACTGG	3 cut(s)	746, 1277, 2032
BseRI	GAGGAG	3 cut(s)	310, 584, 645
BseSI	GKGCMC	2 cut(s)	415, 2224
BseXI	GCAGC	3 cut(s)	1376, 1694, 2108
BseYI	CCCAGC	2 cut(s)	1137, 1950
BshFI	GGCC	7 cut(s)	346, 413, 514, 840, 1303, 1330, 1695
BshNI	GGYRCC	1 cut(s)	1275
BsiHKAI	GWGCWC	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	2191
BsiSI	CCGG	2 cut(s)	113, 2242
BslFI	GGGAC	4 cut(s)	510, 813, 911, 2087
BsmAI	GTCTC	4 cut(s)	70, 193, 1242, 1339
BsmFI	GGGAC	4 cut(s)	510, 813, 911, 2087
BsmI	GAATGC	3 cut(s)	439, 1676, 1703
BsnI	GGCC	7 cut(s)	346, 413, 514, 840, 1303, 1330, 1695
BsoBI	CYCGRG	1 cut(s)	2191
Bsp120I	GGGCCC	1 cut(s)	411
Bsp1286I	GDGCHC	3 cut(s)	127, 415, 2224
Bsp1407I	TGTACA	2 cut(s)	530, 1343
Bsp19I	CCATGG	2 cut(s)	44, 594
BspACI	CCGC	5 cut(s)	360, 454, 457, 582, 1296
BspANI	GGCC	7 cut(s)	346, 413, 514, 840, 1303, 1330, 1695
BspCNI	CTCAG	6 cut(s)	139, 250, 322, 683, 867, 1349
BspHI	TCATGA	2 cut(s)	1429, 1707
BspLI	GGNNCC	8 cut(s)	412, 413, 829, 899, 1129, 1277, 2075, 2076
BspMAI	CTGCAG	1 cut(s)	779
BspMI	ACCTGC	2 cut(s)	798, 991
BspPI	GGATC	6 cut(s)	445, 1041, 1122, 1135, 1590, 2074
BspQI	GCTCTTC	3 cut(s)	111, 963, 1900
BspT107I	GGYRCC	1 cut(s)	1275
BspTI	CTTAAG	1 cut(s)	1205
BsrDI	GCAATG	2 cut(s)	1531, 1570
BsrGI	TGTACA	2 cut(s)	530, 1343
BsrI	ACTGG	3 cut(s)	746, 1277, 2032
BssECI	CCNNGG	7 cut(s)	44, 594, 625, 754, 956, 957, 1721
BssNAI	GTATAC	2 cut(s)	787, 1987
BssNI	GRCGYC	2 cut(s)	591, 1481
BssSI	CACGAG	1 cut(s)	434
BssT1I	CCWWGG	2 cut(s)	44, 594
Bst1107I	GTATAC	2 cut(s)	787, 1987
Bst2BI	CACGAG	1 cut(s)	434
Bst2UI	CCWGG	6 cut(s)	348, 381, 756, 958, 1606, 1722
Bst4CI	ACNGT	2 cut(s)	626, 1280
Bst6I	CTCTTC	5 cut(s)	25, 111, 327, 963, 1900
BstACI	GRCGYC	2 cut(s)	591, 1481
BstAFI	CTTAAG	1 cut(s)	1205
BstAPI	GCANNNNNTGC	1 cut(s)	1754
BstAUI	TGTACA	2 cut(s)	530, 1343
BstBAI	YACGTR	2 cut(s)	547, 1666
BstC8I	GCNNGC	1 cut(s)	123
BstDEI	CTNAG	9 cut(s)	126, 237, 330, 670, 854, 1265, 1290, 1336, 1929
BstDSI	CCRYGG	3 cut(s)	44, 594, 625
BstEII	GGTNACC	1 cut(s)	38
BstF5I	GGATG	9 cut(s)	340, 583, 642, 700, 786, 1147, 1304, 1782, 2036
BstHHI	GCGC	2 cut(s)	920, 1216
BstMAI	GTCTC	4 cut(s)	70, 193, 1242, 1339
BstMWI	GCNNNNNNNGC	7 cut(s)	419, 436, 1160, 1415, 1754, 1930, 2042
BstNI	CCWGG	6 cut(s)	348, 381, 756, 958, 1606, 1722
BstNSI	RCATGY	5 cut(s)	1000, 1534, 1752, 1914, 2018
BstPI	GGTNACC	1 cut(s)	38
BstSCI	CCNGG	7 cut(s)	346, 379, 754, 956, 1604, 1720, 2241
BstSFI	CTRYAG	3 cut(s)	775, 1457, 2197
BstSLI	GKGCMC	2 cut(s)	415, 2224
BstV1I	GCAGC	3 cut(s)	1376, 1694, 2108
BstV2I	GAAGAC	1 cut(s)	2155
BstX2I	RGATCY	3 cut(s)	450, 1127, 1595
BstYI	RGATCY	3 cut(s)	450, 1127, 1595
BstZ17I	GTATAC	2 cut(s)	787, 1987
BsuRI	GGCC	7 cut(s)	346, 413, 514, 840, 1303, 1330, 1695
BtgI	CCRYGG	3 cut(s)	44, 594, 625
BtrI	CACGTC	2 cut(s)	70, 143
BtsCI	GGATG	9 cut(s)	340, 583, 642, 700, 786, 1147, 1304, 1782, 2036
BtsI	GCAGTG	2 cut(s)	707, 772
BtsIMutI	CAGTG	3 cut(s)	707, 772, 2025
BveI	ACCTGC	2 cut(s)	798, 991
Cac8I	GCNNGC	1 cut(s)	123
CciI	TCATGA	2 cut(s)	1429, 1707
CfoI	GCGC	2 cut(s)	920, 1216
Cfr13I	GGNCC	8 cut(s)	362, 411, 412, 512, 827, 898, 1302, 2074
CseI	GACGC	1 cut(s)	599
DdeI	CTNAG	9 cut(s)	126, 237, 330, 670, 854, 1265, 1290, 1336, 1929
Eam1104I	CTCTTC	5 cut(s)	25, 111, 327, 963, 1900
EarI	CTCTTC	5 cut(s)	25, 111, 327, 963, 1900
EciI	GGCGGA	2 cut(s)	443, 1311
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	2 cut(s)	44, 594
Eco147I	AGGCCT	1 cut(s)	1695
Eco24I	GRGCYC	2 cut(s)	127, 415
Eco32I	GATATC	1 cut(s)	1645
Eco47I	GGWCC	4 cut(s)	362, 827, 898, 2074
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	4 cut(s)	32, 188, 308, 1371
Eco88I	CYCGRG	1 cut(s)	2191
Eco91I	GGTNACC	1 cut(s)	38
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	3 cut(s)	411, 412, 2074
EcoO65I	GGTNACC	1 cut(s)	38
EcoRI	GAATTC	1 cut(s)	1039
EcoRII	CCWGG	6 cut(s)	346, 379, 754, 956, 1604, 1720
EcoRV	GATATC	1 cut(s)	1645
EcoT14I	CCWWGG	2 cut(s)	44, 594
EcoT38I	GRGCYC	2 cut(s)	127, 415
ErhI	CCWWGG	2 cut(s)	44, 594
FalI	AAGNNNNNCTT	2 cut(s)	1152, 1184
FaqI	GGGAC	4 cut(s)	510, 813, 911, 2087
FauNDI	CATATG	2 cut(s)	1383, 1582
FbaI	TGATCA	3 cut(s)	1420, 1617, 2109
FblI	GTMKAC	2 cut(s)	786, 1986
Fnu4HI	GCNGC	4 cut(s)	457, 1365, 1683, 2097
FokI	GGATG	9 cut(s)	347, 590, 629, 707, 793, 1154, 1311, 1769, 2043
FriOI	GRGCYC	2 cut(s)	127, 415
Fsp4HI	GCNGC	4 cut(s)	457, 1365, 1683, 2097
FspBI	CTAG	1 cut(s)	1869
FspI	TGCGCA	1 cut(s)	919
GlaI	GCGC	2 cut(s)	919, 1215
GluI	GCNGC	4 cut(s)	457, 1365, 1683, 2097
GsaI	CCCAGC	2 cut(s)	1141, 1954
GsuI	CTGGAG	1 cut(s)	1299
HaeIII	GGCC	7 cut(s)	346, 413, 514, 840, 1303, 1330, 1695
HapII	CCGG	2 cut(s)	113, 2242
HgaI	GACGC	1 cut(s)	599
HhaI	GCGC	2 cut(s)	920, 1216
Hin1I	GRCGYC	2 cut(s)	591, 1481
Hin6I	GCGC	2 cut(s)	918, 1214
HinP1I	GCGC	2 cut(s)	918, 1214
HinfI	GANTC	6 cut(s)	319, 616, 1071, 1549, 1940, 2160
HpaII	CCGG	2 cut(s)	113, 2242
HphI	GGTGA	6 cut(s)	253, 362, 596, 614, 875, 2078
Hpy166II	GTNNAC	4 cut(s)	751, 787, 1987, 2023
Hpy8I	GTNNAC	4 cut(s)	751, 787, 1987, 2023
Hpy99I	CGWCG	1 cut(s)	74
HpyAV	CCTTC	9 cut(s)	7, 17, 503, 1204, 1315, 1346, 1354, 1685, 1994
HpyCH4III	ACNGT	2 cut(s)	626, 1280
HpyCH4IV	ACGT	5 cut(s)	69, 142, 546, 1481, 1665
HpyF10VI	GCNNNNNNNGC	7 cut(s)	419, 436, 1160, 1415, 1754, 1930, 2042
HpyF3I	CTNAG	9 cut(s)	126, 237, 330, 670, 854, 1265, 1290, 1336, 1929
HpySE526I	ACGT	5 cut(s)	69, 142, 546, 1481, 1665
Hsp92I	GRCGYC	2 cut(s)	591, 1481
HspAI	GCGC	2 cut(s)	918, 1214
KflI	GGGWCCC	1 cut(s)	2074
KpnI	GGTACC	1 cut(s)	1279
Ksp22I	TGATCA	3 cut(s)	1420, 1617, 2109
LguI	GCTCTTC	3 cut(s)	111, 963, 1900
LmnI	GCTCC	1 cut(s)	226
Lsp1109I	GCAGC	3 cut(s)	1376, 1694, 2108
LweI	GCATC	6 cut(s)	409, 1423, 1586, 1735, 1791, 2098
MaeI	CTAG	1 cut(s)	1869
MaeII	ACGT	5 cut(s)	69, 142, 546, 1481, 1665
MflI	RGATCY	3 cut(s)	450, 1127, 1595
MhlI	GDGCHC	3 cut(s)	127, 415, 2224
MlyI	GAGTC	3 cut(s)	313, 1543, 2169
MmeI	TCCRAC	2 cut(s)	1590, 1810
MroXI	GAANNNNTTC	1 cut(s)	935
MseI	TTAA	6 cut(s)	194, 267, 1206, 1440, 1698, 1862
MslI	CAYNNNNRTG	2 cut(s)	56, 1054
MspA1I	CMGCKG	3 cut(s)	1349, 1418, 1628
MspCI	CTTAAG	1 cut(s)	1205
MspI	CCGG	2 cut(s)	113, 2242
MspR9I	CCNGG	7 cut(s)	348, 381, 756, 958, 1606, 1722, 2243
Mva1269I	GAATGC	3 cut(s)	439, 1676, 1703
MvaI	CCWGG	6 cut(s)	348, 381, 756, 958, 1606, 1722
MwoI	GCNNNNNNNGC	7 cut(s)	419, 436, 1160, 1415, 1754, 1930, 2042
NciI	CCSGG	1 cut(s)	2243
NcoI	CCATGG	2 cut(s)	44, 594
NdeI	CATATG	2 cut(s)	1383, 1582
NlaIV	GGNNCC	8 cut(s)	412, 413, 829, 899, 1129, 1277, 2075, 2076
NmuCI	GTSAC	4 cut(s)	350, 770, 1280, 1477
NsbI	TGCGCA	1 cut(s)	919
NspI	RCATGY	5 cut(s)	1000, 1534, 1752, 1914, 2018
OliI	CACNNNNGTG	1 cut(s)	56
PagI	TCATGA	2 cut(s)	1429, 1707
PasI	CCCWGGG	1 cut(s)	957
PceI	AGGCCT	1 cut(s)	1695
PciI	ACATGT	2 cut(s)	1530, 2014
PciSI	GCTCTTC	3 cut(s)	111, 963, 1900
PcsI	WCGNNNNNNNCGW	1 cut(s)	160
PctI	GAATGC	3 cut(s)	439, 1676, 1703
PdmI	GAANNNNTTC	1 cut(s)	935
PfeI	GAWTC	3 cut(s)	616, 1071, 1940
PflMI	CCANNNNNTGG	1 cut(s)	1322
PkrI	GCNGC	4 cut(s)	458, 1366, 1684, 2098
PleI	GAGTC	3 cut(s)	313, 1543, 2168
PpsI	GAGTC	3 cut(s)	313, 1543, 2168
Ppu21I	YACGTR	2 cut(s)	547, 1666
PpuMI	RGGWCCY	1 cut(s)	2074
PscI	ACATGT	2 cut(s)	1530, 2014
PshBI	ATTAAT	2 cut(s)	194, 267
PsiI	TTATAA	1 cut(s)	468
Psp124BI	GAGCTC	1 cut(s)	127
Psp5II	RGGWCCY	1 cut(s)	2074
Psp6I	CCWGG	6 cut(s)	346, 379, 754, 956, 1604, 1720
PspEI	GGTNACC	1 cut(s)	38
PspFI	CCCAGC	2 cut(s)	1137, 1950
PspGI	CCWGG	6 cut(s)	346, 379, 754, 956, 1604, 1720
PspN4I	GGNNCC	8 cut(s)	412, 413, 829, 899, 1129, 1277, 2075, 2076
PspOMI	GGGCCC	1 cut(s)	411
PspPI	GGNCC	8 cut(s)	362, 411, 412, 512, 827, 898, 1302, 2074
PspPPI	RGGWCCY	1 cut(s)	2074
PstI	CTGCAG	1 cut(s)	779
PsuI	RGATCY	3 cut(s)	450, 1127, 1595
PvuII	CAGCTG	3 cut(s)	1349, 1418, 1628
RseI	CAYNNNNRTG	2 cut(s)	56, 1054
SacI	GAGCTC	1 cut(s)	127
SapI	GCTCTTC	3 cut(s)	111, 963, 1900
SaqAI	TTAA	6 cut(s)	194, 267, 1206, 1440, 1698, 1862
SatI	GCNGC	4 cut(s)	457, 1365, 1683, 2097
Sau96I	GGNCC	8 cut(s)	362, 411, 412, 512, 827, 898, 1302, 2074
ScaI	AGTACT	1 cut(s)	675
SchI	GAGTC	3 cut(s)	313, 1543, 2169
ScrFI	CCNGG	7 cut(s)	348, 381, 756, 958, 1606, 1722, 2243
SduI	GDGCHC	3 cut(s)	127, 415, 2224
SfaNI	GCATC	6 cut(s)	409, 1423, 1586, 1735, 1791, 2098
SfcI	CTRYAG	3 cut(s)	775, 1457, 2197
SinI	GGWCC	4 cut(s)	362, 827, 898, 2074
SmiMI	CAYNNNNRTG	2 cut(s)	56, 1054
SmlI	CTYRAG	2 cut(s)	146, 1205
SmoI	CTYRAG	2 cut(s)	146, 1205
SseBI	AGGCCT	1 cut(s)	1695
SsiI	CCGC	5 cut(s)	360, 454, 457, 582, 1296
SspI	AATATT	1 cut(s)	2206
SspMI	CTAG	1 cut(s)	1869
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	1 cut(s)	1695
StyD4I	CCNGG	7 cut(s)	346, 379, 754, 956, 1604, 1720, 2241
StyI	CCWWGG	2 cut(s)	44, 594
TaaI	ACNGT	2 cut(s)	626, 1280
TaiI	ACGT	5 cut(s)	72, 145, 549, 1484, 1668
TaqI	TCGA	8 cut(s)	220, 317, 760, 1776, 1938, 1998, 2092, 2236
TatI	WGTACW	6 cut(s)	518, 530, 673, 748, 1343, 1490
TauI	GCSGC	1 cut(s)	459
TfiI	GAWTC	3 cut(s)	616, 1071, 1940
Tru1I	TTAA	6 cut(s)	194, 267, 1206, 1440, 1698, 1862
Tru9I	TTAA	6 cut(s)	194, 267, 1206, 1440, 1698, 1862
TscAI	CASTG	3 cut(s)	714, 779, 2032
TseFI	GTSAC	4 cut(s)	350, 770, 1280, 1477
TseI	GCWGC	3 cut(s)	1364, 1682, 2096
Tsp45I	GTSAC	4 cut(s)	350, 770, 1280, 1477
TspGWI	ACGGA	1 cut(s)	391
TspRI	CASTG	3 cut(s)	714, 779, 2032
Van91I	CCANNNNNTGG	1 cut(s)	1322
Vha464I	CTTAAG	1 cut(s)	1205
VpaK11BI	GGWCC	4 cut(s)	362, 827, 898, 2074
VspI	ATTAAT	2 cut(s)	194, 267
XapI	RAATTY	2 cut(s)	941, 1039
XceI	RCATGY	5 cut(s)	1000, 1534, 1752, 1914, 2018
XcmI	CCANNNNNNNNNTGG	3 cut(s)	762, 1323, 1793
XmiI	GTMKAC	2 cut(s)	786, 1986
XmnI	GAANNNNTTC	1 cut(s)	935
XspI	CTAG	1 cut(s)	1869
ZraI	GACGTC	1 cut(s)	1482
ZrmI	AGTACT	1 cut(s)	675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.