Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005243

Physical Location & Seq

Genomic Coordinates Forward (+)

25044584 .. 25058376

Gene Structure 13793 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1237700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGTGGAGGTTGAAGCTTTCAGAAGGCAGAGAGCCATGGCTTACAAGTGTGAACAATCACGTCGGAAGACAATATTGGGAGTTTGATCCAAACCTCGGAACACCGGAAGAGAGAGCTCAAGTGGACAAAGTCCGTCTTGAGTTTCACAAGAATCGTTTTCGCACAAAACATAGTTCCGATCTCCTAATGAGACTTCAGGAAGCAAGTGAAAAGAAGCAAGTAGTATCGGATCAGGTGAAAGTGAAAAGTGGTGACGAGGAGGAGGAAGTAATAAGCGAGGAAGCAGTGGAAATCACGTTGAGGAGGGCGTTGCGATATTACTCAACTCTGCAGGCTGACGATGGTTTCTGGCCTGGTGACTATGGTGGACCTTTGTTTCTTCTACCTGGCTTGGTTATTGGCTTATATGTAACTGGGGCCCTTCATACAATCTTGCCGCATGACCAGCATCAACAGGAGATCTGCCGCTATCTGTATAACCATCAGAATATAGATGGAGGTTGGGGACTACATATAGAAGGGCCCAGCACAATGTTTTGTACAGCTCTGTCCTATGTCACCTTGAGATTGCTTGGAGAGGAAATTGACGGTGGTGATGGCGCAATGGATAAGGCAAGAAAATGGATTCTTGATCGTGGTGGAGTTACCTTCATACCATCATGGGGAAAGATGTGGCTTTCAGTGCTTGGTGTATATGAATGGAGCGGAAACAATCCTCTGCCTCCAGAACTGTGGCTTTTACCTTACTCTCTCCCAATACACCCAGGTCGAATGTGGTGTCACTGTAGGATGGTTTATCTGCCCATGTCATATTTATACGGAAAGAGATTTGTAGGTCCTATCGACGGCCTTATTTTATCTCTCAGGAGAGAACTTTATACCTTTTCCTATCACCTGATTGATTGGGATCAAGCAAGAAATCTATGTGCTAAGGAGGATTTGTATTACCCACATCCCCTTTTACAGGACATGTTGTGGGGATCTCTTCATAAGGTTGGGGAGCCTCTTCTGAAGGGATGGCCATTCTCTAAACTAAGGCAAAAGGCTCTCCGCACTATAATGCAACACATACACTATGAGGATGAAAATACTCAATATATTTGCATTGGACCTGTCAATAAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCCAAATTCAAAACCATACAAGCATCATCTCTCAAGAATCAAAGATTATCTTTGGGTTGCTGAAGATGGAATGAAGATGCAGGGATATAATGGATCTCAATGTTGGGATGTTGCTTTGGCTGTTCAAGCAATCCTTGCAACCAACCTCTTTACTGAATCTGTTTCCATGCTTAAGAAGGCGCACGATTTTATAAAAAACACACAAGTTCGGACAAACAGCTCCAGAAACCCTAGTAACTGGTACCGTCACATTTCCAAGGGTGGATGGCCCTTCTCCACTCCAGACAATGGCTGGCCTGTCTCAGATTGTACGGCAGAAGGTTTGAAGGCAGCCCTCTTACTATCAAGTATACCGTCTGAAATAGTAGGAGAAGCCGTACAGACTGATCACCTATATGATGCTGTTAATGTGATATTATCCCTACAGAACAGTACTGGAGGATTTGCATCCTATGAACTCACAAGATCTTACCCCTGGTTGGAGATGATCAATCCAGCTGAAACATTCGGAGATATCATCATTGACTATCAGTACGTGGAATGCACATCAGCAGCAATACAAGGCCTTAAATCATTCATGAAGTTATATCCAGGACATAGGAGGAAAGAAATATTAGCATGTATTGCAAAGGCAGTCAAATTCATCGAGAGCATACAACAACCTGATGGCTCATGGTATGGGTCTTGGGGAGTCTGCTACACCTATGGAACATGGTTTGGGATAAAGGGGCTGGTGGCTGGCGGTAAGACATACCAAACAAGTCATAGCATTAGAAAAGCATGTGATTTCTTGCTGTCCAAACAACTTGATTCAGGCGGCTGGGGAGAGAGCTACCTTTCATGCCAAAACAAGGTATACACAAACCTGGAAGGGAACAAGCCACATATAGTGAACACTGGATGGGCAATGCTGGGTCTTATTGATGCAGGGCAGGCTGAGAGGGACCCTGCGGCCTTGCACCGTGCATCAAAGCTGTTGATTAATTCACAGATGGAGAATGGAGATTTCCCACAACAGCAAATCATGGGAGTGTTTAACAAGAACTGCATGATCAGTTATTCTGCGTACAGAAACATCTTCCCCATGTGGGCCCTTGGAGAGTATCGCAATCGCGTATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

759

Amino Acids

86.54

Weight (kDa)

6.67

Isoelectric Point (pI)

42.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	141 - 404	4.6e-36	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	417 - 755	4.5e-51	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45130 AT3G45130 AT3G45130 AT3G45130 AT3G45130
fragaria_vesca	FvH4_4g06000
malus_domestica	MD16G1237700.v1.1
prunus_persica	Prupe.1G063500_v2.0.a1 Prupe.1G063500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr0c45g0503831 RchiOBHm_Chr1g0332701 RchiOBHm_Chr4g0397241 RchiOBHm_Chr4g0397271 RchiOBHm_Chr4g0397301 RchiOBHm_Chr4g0397321
rosa_laevigata	RLG00000009434 RLG00000009437
rosa_multiflora	Rmu_sc0003239.1_g000001 Rmu_sc0003929.1_g000015 Rmu_sc0008546.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342180 Rroxscaffold_5G00342240 Rroxscaffold_5G00342320
rosa_rugosa	Rorug03G0343000 Rorug03G0343900 Rorug03G0343900
rosa_wichuraiana	Rw4G006330 Rw4G006340 Rw4G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1346
Acc65I	GGTACC	1 cut(s)	1395
AccB1I	GGYRCC	1 cut(s)	1395
AccB7I	CCANNNNNTGG	1 cut(s)	1442
AccBSI	CCGCTC	1 cut(s)	705
AccI	GTMKAC	2 cut(s)	1504, 2010
AccII	CGCG	1 cut(s)	2268
AciI	CCGC	7 cut(s)	437, 466, 705, 1051, 1896, 1971, 2105
AclWI	GGATC	7 cut(s)	80, 237, 915, 988, 1148, 1161, 1255
AcoI	YGGCCR	1 cut(s)	1019
AcsI	RAATTY	2 cut(s)	1159, 1793
AcuI	CTGAAG	3 cut(s)	179, 1031, 1236
AfaI	GTAC	8 cut(s)	541, 1125, 1397, 1465, 1533, 1588, 1688, 2222
AfiI	CCNNNNNNNGG	8 cut(s)	95, 662, 845, 964, 1442, 1633, 2005, 2242
AflII	CTTAAG	1 cut(s)	1325
AflIII	ACRYGT	1 cut(s)	969
AgsI	TTSAA	4 cut(s)	13, 1164, 1280, 1480
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
AjuI	GAANNNNNNNTTGG	2 cut(s)	58, 90
AluBI	AGCT	7 cut(s)	16, 116, 545, 1374, 1652, 1986, 2128
AluI	AGCT	7 cut(s)	16, 116, 545, 1374, 1652, 1986, 2128
Alw21I	GWGCWC	1 cut(s)	118
Alw26I	GTCTC	2 cut(s)	184, 1459
AlwI	GGATC	7 cut(s)	80, 237, 915, 988, 1148, 1161, 1255
ApaI	GGGCCC	3 cut(s)	421, 525, 2248
ApeKI	GCWGC	2 cut(s)	1484, 1706
ApoI	RAATTY	2 cut(s)	1159, 1793
ArsI	GACNNNNNNTTYG	2 cut(s)	763, 795
AseI	ATTAAT	1 cut(s)	2136
Asp718I	GGTACC	1 cut(s)	1395
AspLEI	GCGC	2 cut(s)	602, 1336
AsuHPI	GGTGA	7 cut(s)	247, 263, 368, 550, 605, 884, 1535
AvaII	GGWCC	4 cut(s)	368, 836, 1109, 2098
BaeGI	GKGCMC	3 cut(s)	421, 525, 2248
BalI	TGGCCA	1 cut(s)	1021
BamHI	GGATCC	1 cut(s)	1153
BanI	GGYRCC	1 cut(s)	1395
BanII	GRGCYC	4 cut(s)	118, 421, 525, 2248
BbsI	GAAGAC	1 cut(s)	73
Bbv12I	GWGCWC	1 cut(s)	118
BbvI	GCAGC	2 cut(s)	1496, 1718
BceAI	ACGGC	3 cut(s)	862, 1482, 1514
BciT130I	CCWGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
BclI	TGATCA	3 cut(s)	1540, 1641, 2205
BcoDI	GTCTC	2 cut(s)	184, 1459
BfaI	CTAG	1 cut(s)	1386
BfmI	CTRYAG	3 cut(s)	329, 784, 1577
BfrI	CTTAAG	1 cut(s)	1325
BglII	AGATCT	2 cut(s)	459, 1619
BisI	GCNGC	6 cut(s)	437, 466, 1485, 1707, 1972, 2106
BlsI	GCNGC	6 cut(s)	438, 467, 1486, 1708, 1973, 2107
BmcAI	AGTACT	1 cut(s)	1588
Bme1390I	CCNGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
Bme18I	GGWCC	4 cut(s)	368, 836, 1109, 2098
BmgBI	CACGTC	1 cut(s)	61
BmiI	GGNNCC	9 cut(s)	418, 419, 523, 1002, 1155, 1397, 2099, 2100, 2246
BmrFI	CCNGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
BmrI	ACTGGG	1 cut(s)	423
BmsI	GCATC	7 cut(s)	457, 1186, 1221, 1543, 1610, 2068, 2129
BmuI	ACTGGG	1 cut(s)	423
BpiI	GAAGAC	1 cut(s)	73
BpmI	CTGGAG	4 cut(s)	708, 1360, 1419, 1611
Bpu10I	CCTNAGC	1 cut(s)	930
BpuEI	CTTGAG	4 cut(s)	102, 158, 583, 1171
BsaAI	YACGTR	1 cut(s)	1690
BsaBI	GATNNNNATC	2 cut(s)	906, 1600
BsaJI	CCNNGG	6 cut(s)	35, 94, 763, 1410, 1628, 2248
BsaWI	WCCGGW	1 cut(s)	103
BsaXI	ACNNNNNCTCC	4 cut(s)	926, 956, 1515, 1545
Bsc4I	CCNNNNNNNGG	8 cut(s)	95, 662, 845, 964, 1442, 1633, 2005, 2242
Bse1I	ACTGG	4 cut(s)	418, 1397, 1594, 2056
Bse3DI	GCAATG	2 cut(s)	609, 2067
Bse8I	GATNNNNATC	2 cut(s)	906, 1600
BseBI	CCWGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
BseDI	CCNNGG	6 cut(s)	35, 94, 763, 1410, 1628, 2248
BseGI	GGATG	8 cut(s)	795, 952, 1022, 1087, 1267, 1424, 1601, 2060
BseJI	GATNNNNATC	2 cut(s)	906, 1600
BseLI	CCNNNNNNNGG	8 cut(s)	95, 662, 845, 964, 1442, 1633, 2005, 2242
BseMI	GCAATG	2 cut(s)	609, 2067
BseMII	CTCAG	3 cut(s)	877, 1470, 2082
BseNI	ACTGG	4 cut(s)	418, 1397, 1594, 2056
BseRI	GAGGAG	3 cut(s)	272, 275, 316
BseSI	GKGCMC	3 cut(s)	421, 525, 2248
BseXI	GCAGC	2 cut(s)	1496, 1718
BseYI	CCCAGC	3 cut(s)	524, 1974, 2065
Bsh1236I	CGCG	1 cut(s)	2268
BshNI	GGYRCC	1 cut(s)	1395
BsiHKAI	GWGCWC	1 cut(s)	118
BsiSI	CCGG	1 cut(s)	104
BslFI	GGGAC	2 cut(s)	519, 2111
BslI	CCNNNNNNNGG	8 cut(s)	95, 662, 845, 964, 1442, 1633, 2005, 2242
BsmAI	GTCTC	2 cut(s)	184, 1459
BsmFI	GGGAC	2 cut(s)	519, 2111
BsmI	GAATGC	1 cut(s)	1700
Bsp120I	GGGCCC	3 cut(s)	417, 521, 2244
Bsp1286I	GDGCHC	4 cut(s)	118, 421, 525, 2248
Bsp1407I	TGTACA	1 cut(s)	539
Bsp19I	CCATGG	1 cut(s)	35
BspACI	CCGC	7 cut(s)	437, 466, 705, 1051, 1896, 1971, 2105
BspCNI	CTCAG	3 cut(s)	876, 1469, 2083
BspFNI	CGCG	1 cut(s)	2268
BspHI	TCATGA	1 cut(s)	1731
BspLI	GGNNCC	9 cut(s)	418, 419, 523, 1002, 1155, 1397, 2099, 2100, 2246
BspMAI	CTGCAG	1 cut(s)	333
BspPI	GGATC	7 cut(s)	80, 237, 915, 988, 1148, 1161, 1255
BspT107I	GGYRCC	1 cut(s)	1395
BspTI	CTTAAG	1 cut(s)	1325
BsrBI	CCGCTC	1 cut(s)	705
BsrDI	GCAATG	2 cut(s)	609, 2067
BsrGI	TGTACA	1 cut(s)	539
BsrI	ACTGG	4 cut(s)	418, 1397, 1594, 2056
BssECI	CCNNGG	6 cut(s)	35, 94, 763, 1410, 1628, 2248
BssNAI	GTATAC	2 cut(s)	1505, 2011
BssT1I	CCWWGG	3 cut(s)	35, 1410, 2248
Bst1107I	GTATAC	2 cut(s)	1505, 2011
Bst2UI	CCWGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
Bst4CI	ACNGT	7 cut(s)	590, 732, 785, 1400, 1509, 1586, 2117
Bst6I	CTCTTC	3 cut(s)	102, 990, 1011
BstAFI	CTTAAG	1 cut(s)	1325
BstAPI	GCANNNNNTGC	2 cut(s)	1289, 1778
BstAUI	TGTACA	1 cut(s)	539
BstBAI	YACGTR	1 cut(s)	1690
BstC8I	GCNNGC	4 cut(s)	333, 1448, 1894, 2088
BstDEI	CTNAG	5 cut(s)	863, 930, 1034, 1456, 2091
BstDSI	CCRYGG	1 cut(s)	35
BstENI	CCTNNNNNAGG	1 cut(s)	962
BstF5I	GGATG	8 cut(s)	795, 952, 1022, 1087, 1267, 1424, 1601, 2060
BstFNI	CGCG	1 cut(s)	2268
BstHHI	GCGC	2 cut(s)	602, 1336
BstMAI	GTCTC	2 cut(s)	184, 1459
BstMWI	GCNNNNNNNGC	6 cut(s)	445, 682, 1280, 1289, 1778, 2087
BstNI	CCWGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
BstNSI	RCATGY	3 cut(s)	973, 1776, 1938
BstSCI	CCNGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
BstSFI	CTRYAG	3 cut(s)	329, 784, 1577
BstSLI	GKGCMC	3 cut(s)	421, 525, 2248
BstUI	CGCG	1 cut(s)	2268
BstV1I	GCAGC	2 cut(s)	1496, 1718
BstV2I	GAAGAC	1 cut(s)	73
BstX2I	RGATCY	5 cut(s)	459, 980, 1153, 1247, 1619
BstXI	CCANNNNNNTGG	1 cut(s)	732
BstYI	RGATCY	5 cut(s)	459, 980, 1153, 1247, 1619
BstZ17I	GTATAC	2 cut(s)	1505, 2011
BtgI	CCRYGG	1 cut(s)	35
BtrI	CACGTC	1 cut(s)	61
BtsCI	GGATG	8 cut(s)	795, 952, 1022, 1087, 1267, 1424, 1601, 2060
BtsI	GCAGTG	1 cut(s)	291
BtsIMutI	CAGTG	4 cut(s)	291, 687, 781, 2049
Cac8I	GCNNGC	4 cut(s)	333, 1448, 1894, 2088
CciI	TCATGA	1 cut(s)	1731
CfoI	GCGC	2 cut(s)	602, 1336
Csp6I	GTAC	8 cut(s)	540, 1124, 1396, 1464, 1532, 1587, 1687, 2221
CviQI	GTAC	8 cut(s)	540, 1124, 1396, 1464, 1532, 1587, 1687, 2221
DdeI	CTNAG	5 cut(s)	863, 930, 1034, 1456, 2091
EaeI	YGGCCR	1 cut(s)	1019
Eam1104I	CTCTTC	3 cut(s)	102, 990, 1011
EarI	CTCTTC	3 cut(s)	102, 990, 1011
Ecl136II	GAGCTC	1 cut(s)	116
Eco130I	CCWWGG	3 cut(s)	35, 1410, 2248
Eco147I	AGGCCT	1 cut(s)	1719
Eco24I	GRGCYC	4 cut(s)	118, 421, 525, 2248
Eco32I	GATATC	1 cut(s)	1669
Eco47I	GGWCC	4 cut(s)	368, 836, 1109, 2098
Eco53kI	GAGCTC	1 cut(s)	116
Eco57I	CTGAAG	3 cut(s)	179, 1031, 1236
EcoICRI	GAGCTC	1 cut(s)	116
EcoNI	CCTNNNNNAGG	1 cut(s)	962
EcoO109I	RGGNCCY	6 cut(s)	417, 418, 521, 836, 2098, 2245
EcoRII	CCWGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
EcoRV	GATATC	1 cut(s)	1669
EcoT14I	CCWWGG	3 cut(s)	35, 1410, 2248
EcoT38I	GRGCYC	4 cut(s)	118, 421, 525, 2248
ErhI	CCWWGG	3 cut(s)	35, 1410, 2248
FalI	AAGNNNNNCTT	6 cut(s)	120, 152, 1188, 1220, 1272, 1304
FaqI	GGGAC	2 cut(s)	519, 2111
FbaI	TGATCA	3 cut(s)	1540, 1641, 2205
FblI	GTMKAC	2 cut(s)	1504, 2010
Fnu4HI	GCNGC	6 cut(s)	437, 466, 1485, 1707, 1972, 2106
FokI	GGATG	8 cut(s)	802, 939, 1029, 1094, 1274, 1431, 1588, 2067
FriOI	GRGCYC	4 cut(s)	118, 421, 525, 2248
Fsp4HI	GCNGC	6 cut(s)	437, 466, 1485, 1707, 1972, 2106
FspBI	CTAG	1 cut(s)	1386
GlaI	GCGC	2 cut(s)	601, 1335
GluI	GCNGC	6 cut(s)	437, 466, 1485, 1707, 1972, 2106
GsaI	CCCAGC	3 cut(s)	528, 1978, 2069
GsuI	CTGGAG	4 cut(s)	708, 1360, 1419, 1611
HapII	CCGG	1 cut(s)	104
HhaI	GCGC	2 cut(s)	602, 1336
Hin6I	GCGC	2 cut(s)	600, 1334
HinP1I	GCGC	2 cut(s)	600, 1334
HindIII	AAGCTT	1 cut(s)	14
HinfI	GANTC	6 cut(s)	151, 625, 1191, 1310, 1845, 1964
HpaII	CCGG	1 cut(s)	104
HphI	GGTGA	7 cut(s)	247, 263, 368, 550, 605, 884, 1535
Hpy166II	GTNNAC	6 cut(s)	52, 124, 368, 1505, 2011, 2047
Hpy8I	GTNNAC	6 cut(s)	52, 124, 368, 1505, 2011, 2047
Hpy99I	CGWCG	2 cut(s)	65, 848
HpyCH4III	ACNGT	7 cut(s)	590, 732, 785, 1400, 1509, 1586, 2117
HpyCH4IV	ACGT	3 cut(s)	60, 296, 1689
HpyF10VI	GCNNNNNNNGC	6 cut(s)	445, 682, 1280, 1289, 1778, 2087
HpyF3I	CTNAG	5 cut(s)	863, 930, 1034, 1456, 2091
HpySE526I	ACGT	3 cut(s)	60, 296, 1689
HspAI	GCGC	2 cut(s)	600, 1334
KflI	GGGWCCC	1 cut(s)	2098
KpnI	GGTACC	1 cut(s)	1399
Ksp22I	TGATCA	3 cut(s)	1540, 1641, 2205
LmnI	GCTCC	3 cut(s)	702, 1000, 1379
Lsp1109I	GCAGC	2 cut(s)	1496, 1718
LweI	GCATC	7 cut(s)	457, 1186, 1221, 1543, 1610, 2068, 2129
MaeI	CTAG	1 cut(s)	1386
MaeII	ACGT	3 cut(s)	60, 296, 1689
MaeIII	GTNAC	8 cut(s)	251, 356, 409, 556, 643, 779, 1388, 1400
MbiI	CCGCTC	1 cut(s)	705
MboII	GAAGA	8 cut(s)	78, 119, 371, 977, 998, 1229, 1240, 2224
MflI	RGATCY	5 cut(s)	459, 980, 1153, 1247, 1619
MhlI	GDGCHC	4 cut(s)	118, 421, 525, 2248
MlsI	TGGCCA	1 cut(s)	1021
MluCI	AATT	4 cut(s)	582, 1159, 1793, 2137
MluNI	TGGCCA	1 cut(s)	1021
MlyI	GAGTC	1 cut(s)	1854
MmeI	TCCRAC	2 cut(s)	43, 1614
Mox20I	TGGCCA	1 cut(s)	1021
MscI	TGGCCA	1 cut(s)	1021
MseI	TTAA	5 cut(s)	1326, 1560, 1722, 2136, 2190
MslI	CAYNNNNRTG	3 cut(s)	1058, 1548, 2183
Msp20I	TGGCCA	1 cut(s)	1021
MspA1I	CMGCKG	1 cut(s)	1652
MspCI	CTTAAG	1 cut(s)	1325
MspI	CCGG	1 cut(s)	104
MspR9I	CCNGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
Mva1269I	GAATGC	1 cut(s)	1700
MvaI	CCWGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
MvnI	CGCG	1 cut(s)	2268
MwoI	GCNNNNNNNGC	6 cut(s)	445, 682, 1280, 1289, 1778, 2087
NcoI	CCATGG	1 cut(s)	35
NlaIV	GGNNCC	9 cut(s)	418, 419, 523, 1002, 1155, 1397, 2099, 2100, 2246
NmuCI	GTSAC	5 cut(s)	251, 356, 556, 779, 1400
NspI	RCATGY	3 cut(s)	973, 1776, 1938
PagI	TCATGA	1 cut(s)	1731
PceI	AGGCCT	1 cut(s)	1719
PciI	ACATGT	1 cut(s)	969
PctI	GAATGC	1 cut(s)	1700
PfeI	GAWTC	5 cut(s)	151, 625, 1191, 1310, 1964
PflFI	GACNNNGTC	1 cut(s)	128
PflMI	CCANNNNNTGG	1 cut(s)	1442
PfoI	TCCNGGA	1 cut(s)	1744
PkrI	GCNGC	6 cut(s)	438, 467, 1486, 1708, 1973, 2107
PleI	GAGTC	1 cut(s)	1853
PpsI	GAGTC	1 cut(s)	1853
Ppu21I	YACGTR	1 cut(s)	1690
PpuMI	RGGWCCY	2 cut(s)	836, 2098
PscI	ACATGT	1 cut(s)	969
PshBI	ATTAAT	1 cut(s)	2136
PsiI	TTATAA	1 cut(s)	1346
Psp124BI	GAGCTC	1 cut(s)	118
Psp5II	RGGWCCY	2 cut(s)	836, 2098
Psp6I	CCWGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
PspFI	CCCAGC	3 cut(s)	524, 1974, 2065
PspGI	CCWGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
PspN4I	GGNNCC	9 cut(s)	418, 419, 523, 1002, 1155, 1397, 2099, 2100, 2246
PspOMI	GGGCCC	3 cut(s)	417, 521, 2244
PspPPI	RGGWCCY	2 cut(s)	836, 2098
PstI	CTGCAG	1 cut(s)	333
PsuI	RGATCY	5 cut(s)	459, 980, 1153, 1247, 1619
PsyI	GACNNNGTC	1 cut(s)	128
PvuII	CAGCTG	1 cut(s)	1652
RsaI	GTAC	8 cut(s)	541, 1125, 1397, 1465, 1533, 1588, 1688, 2222
RsaNI	GTAC	8 cut(s)	540, 1124, 1396, 1464, 1532, 1587, 1687, 2221
RseI	CAYNNNNRTG	3 cut(s)	1058, 1548, 2183
SacI	GAGCTC	1 cut(s)	118
SaqAI	TTAA	5 cut(s)	1326, 1560, 1722, 2136, 2190
SatI	GCNGC	6 cut(s)	437, 466, 1485, 1707, 1972, 2106
ScaI	AGTACT	1 cut(s)	1588
SchI	GAGTC	1 cut(s)	1854
ScrFI	CCNGG	6 cut(s)	354, 387, 765, 1630, 1746, 2021
SduI	GDGCHC	4 cut(s)	118, 421, 525, 2248
SfaNI	GCATC	7 cut(s)	457, 1186, 1221, 1543, 1610, 2068, 2129
SfcI	CTRYAG	3 cut(s)	329, 784, 1577
SinI	GGWCC	4 cut(s)	368, 836, 1109, 2098
SmiMI	CAYNNNNRTG	3 cut(s)	1058, 1548, 2183
SmlI	CTYRAG	5 cut(s)	117, 137, 562, 1186, 1325
SmoI	CTYRAG	5 cut(s)	117, 137, 562, 1186, 1325
Sse9I	AATT	4 cut(s)	582, 1159, 1793, 2137
SseBI	AGGCCT	1 cut(s)	1719
SsiI	CCGC	7 cut(s)	437, 466, 705, 1051, 1896, 1971, 2105
SspI	AATATT	2 cut(s)	74, 1767
SspMI	CTAG	1 cut(s)	1386
SstI	GAGCTC	1 cut(s)	118
StuI	AGGCCT	1 cut(s)	1719
StyD4I	CCNGG	6 cut(s)	352, 385, 763, 1628, 1744, 2019
StyI	CCWWGG	3 cut(s)	35, 1410, 2248
TaaI	ACNGT	7 cut(s)	590, 732, 785, 1400, 1509, 1586, 2117
TaiI	ACGT	3 cut(s)	63, 299, 1692
TaqI	TCGA	3 cut(s)	769, 843, 1800
TasI	AATT	4 cut(s)	582, 1159, 1793, 2137
TatI	WGTACW	2 cut(s)	539, 1586
TauI	GCSGC	4 cut(s)	439, 468, 1974, 2108
TfiI	GAWTC	5 cut(s)	151, 625, 1191, 1310, 1964
Tru1I	TTAA	5 cut(s)	1326, 1560, 1722, 2136, 2190
Tru9I	TTAA	5 cut(s)	1326, 1560, 1722, 2136, 2190
TscAI	CASTG	4 cut(s)	291, 687, 788, 2056
TseFI	GTSAC	5 cut(s)	251, 356, 556, 779, 1400
TseI	GCWGC	2 cut(s)	1484, 1706
Tsp45I	GTSAC	5 cut(s)	251, 356, 556, 779, 1400
TspGWI	ACGGA	2 cut(s)	122, 834
TspRI	CASTG	4 cut(s)	291, 687, 788, 2056
Tth111I	GACNNNGTC	1 cut(s)	128
Van91I	CCANNNNNTGG	1 cut(s)	1442
Vha464I	CTTAAG	1 cut(s)	1325
VpaK11BI	GGWCC	4 cut(s)	368, 836, 1109, 2098
VspI	ATTAAT	1 cut(s)	2136
XagI	CCTNNNNNAGG	1 cut(s)	962
XapI	RAATTY	2 cut(s)	1159, 1793
XceI	RCATGY	3 cut(s)	973, 1776, 1938
XcmI	CCANNNNNNNNNTGG	2 cut(s)	771, 1443
XmiI	GTMKAC	2 cut(s)	1504, 2010
XspI	CTAG	1 cut(s)	1386
ZrmI	AGTACT	1 cut(s)	1588

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.