Rroxscaffold_5G00342240

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005243
Physical Location & Seq
Forward (+)
11043627 .. 11049045
5419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342240.1

Sequence Viewer

Length: 1461 bp
ATGGACGGTGGTGATGGCGCCATGGAGAAGGCAAGAAAATGGATTCTTCACCGTGGAGGAGTTACCACCATCCCGTCATGGGGAAAGATGTGGCTCTCAGACGTGCTGTGGGGGTGTCTTCATAAGGTTGGGGAACCACTTCTCATGAGATGGCCATTGTCTAACCTCAGGCAAAAAGCTCTCACCACTGTGATGGACCACATACACTATGAGGATGAAAACACTCAGTATATCTGCATTGGAGCTGTCAATAAGGTACTAAACATGATTTGTTGTTGGGTGGAGGATCAGAATTCAGAAGCGTACAAGCGTCATCTTTCAAGAATCAAAGATTATCTATGGGTTGCAGAAGATGGAATGAAAATGCAGGGATATAATGGATCTCAATGCTGGGACGTTGTTTTGGCTGTTCAAGCAATCCTTTCTACCAACCTTGTGAATGAATATGCTTCCTTGCTTAAGAAGGCGCACTATTTCATCAAAAATACACAAGTTAGGAGAGACAGCTATGGCAATCTGCGTTACTGGTACCGTCACATTTCTAAGGGCGGATGGCCCTTCTCCACTCCAGACAATGGTTGGCCTGCTTCAGATTGTACAGCTGAAGGTTTGAAGGCAGCACTCTTATTATCACGTATGCCCTGCGAAATTGTGGGAGAAGCAATAGCAGCTGATCAACTGCATGATGCTGTTAATGTAATATTATCCCTACAGAATAGCAATGGAGGGTTTGCATCATATGAACTCACAAGATCGTATGCCTGGTTGGAGATGATCAATCCAGCTGAAACATTTGGAGATGTCATGAATGATTATCAGTCTGTGGAATGCACATCATCAGCAGTAGAAGGCCTTAATGCATTCATGAAATTATATACAGGGCATCGGAAGAAAGATATAGAAGCATGTATTGCAAAGGCAGTTCAGTTCATCGAGAGCATACAACTTCCTGATGGCTCATGGTATGGGACTTGGGGAGTTTGCTACACCTATGGAACATGGTTTGGAATAAAGGGGTTAATGGCTGGTGGCAAGACCTATCAGACAAGTAATAGCATTAGAAGAGCATGTCATTTCTTGCTATCTAAGCAACTCGATTCAGGTGGCTGGGGAGAGAGCTTTCTCTCATGCCAAAACGAGGTATACACAAATCTCGAAGGGAACAAACCACACATAGTAAACACTGGATGGGCTATGTTGGCTCTAATTGAAGCAGGGCAGGGTGAAAGGGACCCGATCCCCTTGCATCGAGCAGCAAAGGTATTGATCAATTCACAGATGGAAAATGGAGATTTCCCACAACAGCAAATCATGGGAGTCTTCAACAAAAACTGCATGATTAGTTACTCCGCCTACAGAAATATCTTTCCCATCTGGGCACTTGGAGAGTATCGAAACCGGTGTATTAGTGGCTGTGCAAATTTGATCGTGTTTATCAAGACCATTTTCAGGAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

486

Amino Acids

54.86

Weight (kDa)

7.5

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 128 - 466 2.5e-53 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 2 cut(s) 17, 528
AccB7I CCANNNNNTGG 1 cut(s) 575
AccI GTMKAC 1 cut(s) 1143
AciI CCGC 2 cut(s) 549, 1350
AclWI GGATC 3 cut(s) 294, 388, 1231
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 2 cut(s) 292, 1420
AcuI CTGAAG 2 cut(s) 573, 624
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 4 cut(s) 258, 305, 530, 598
AfiI CCNNNNNNNGG 5 cut(s) 79, 80, 575, 1138, 1449
AflII CTTAAG 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 1398
AgsI TTSAA 5 cut(s) 321, 413, 613, 1211, 1324
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 761
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 7 cut(s) 179, 245, 507, 602, 671, 785, 1119
AluI AGCT 7 cut(s) 179, 245, 507, 602, 671, 785, 1119
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 3 cut(s) 294, 388, 1231
AoxI GGCC 4 cut(s) 152, 554, 581, 850
ApeKI GCWGC 3 cut(s) 617, 668, 1253
ApoI RAATTY 2 cut(s) 292, 1420
AsiGI ACCGGT 1 cut(s) 1398
Asp700I GAANNNNTTC 1 cut(s) 138
Asp718I GGTACC 1 cut(s) 528
AspLEI GCGC 2 cut(s) 20, 469
AspS9I GGNCC 3 cut(s) 196, 555, 1231
AsuHPI GGTGA 4 cut(s) 23, 41, 175, 1235
AvaII GGWCC 2 cut(s) 196, 1231
AxyI CCTNAGG 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 1381
BalI TGGCCA 1 cut(s) 154
BanI GGYRCC 2 cut(s) 17, 528
BbsI GAAGAC 2 cut(s) 110, 1312
BbvI GCAGC 3 cut(s) 629, 680, 1265
BcgI CGANNNNNNTGC 2 cut(s) 1225, 1259
BciT130I CCWGG 1 cut(s) 763
BclI TGATCA 3 cut(s) 673, 774, 1266
BcoDI GTCTC 1 cut(s) 495
BfmI CTRYAG 2 cut(s) 710, 1354
BfoI RGCGCY 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 458
BisI GCNGC 3 cut(s) 618, 669, 1254
BlsI GCNGC 3 cut(s) 619, 670, 1255
Bme1390I CCNGG 1 cut(s) 763
Bme18I GGWCC 2 cut(s) 196, 1231
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 196, 555, 1231
BmiI GGNNCC 5 cut(s) 19, 135, 530, 1232, 1233
BmrFI CCNGG 1 cut(s) 763
BmsI GCATC 4 cut(s) 676, 743, 892, 1255
BpiI GAAGAC 2 cut(s) 110, 1312
BpmI CTGGAG 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 21, 52
BsaWI WCCGGW 1 cut(s) 1398
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 80, 575, 1138, 1449
Bse118I RCCGGY 1 cut(s) 1398
Bse1I ACTGG 2 cut(s) 530, 1189
Bse21I CCTNAGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 727
BseBI CCWGG 1 cut(s) 763
BseDI CCNNGG 2 cut(s) 21, 52
BseGI GGATG 4 cut(s) 69, 220, 557, 1193
BseLI CCNNNNNNNGG 5 cut(s) 79, 80, 575, 1138, 1449
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 3 cut(s) 111, 181, 239
BseNI ACTGG 2 cut(s) 530, 1189
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 1381
BseXI GCAGC 3 cut(s) 629, 680, 1265
BseYI CCCAGC 2 cut(s) 390, 1107
BshFI GGCC 4 cut(s) 154, 556, 583, 852
BshNI GGYRCC 2 cut(s) 17, 528
BshTI ACCGGT 1 cut(s) 1398
BsiSI CCGG 1 cut(s) 1399
BslFI GGGAC 3 cut(s) 407, 982, 1244
BslI CCNNNNNNNGG 5 cut(s) 79, 80, 575, 1138, 1449
BsmAI GTCTC 1 cut(s) 495
BsmFI GGGAC 3 cut(s) 407, 982, 1244
BsmI GAATGC 2 cut(s) 833, 860
BsnI GGCC 4 cut(s) 154, 556, 583, 852
Bsp1286I GDGCHC 1 cut(s) 1381
Bsp1407I TGTACA 1 cut(s) 596
Bsp143I GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 2 cut(s) 549, 1350
BspANI GGCC 4 cut(s) 154, 556, 583, 852
BspCNI CTCAG 3 cut(s) 110, 180, 238
BspHI TCATGA 3 cut(s) 144, 804, 864
BspLI GGNNCC 5 cut(s) 19, 135, 530, 1232, 1233
BspPI GGATC 3 cut(s) 294, 388, 1231
BspQI GCTCTTC 1 cut(s) 1057
BspT107I GGYRCC 2 cut(s) 17, 528
BspTI CTTAAG 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 1398
BsrGI TGTACA 1 cut(s) 596
BsrI ACTGG 2 cut(s) 530, 1189
BssAI RCCGGY 1 cut(s) 1398
BssECI CCNNGG 2 cut(s) 21, 52
BssMI GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
BssNAI GTATAC 1 cut(s) 1144
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 21
Bst1107I GTATAC 1 cut(s) 1144
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 4 cut(s) 8, 53, 190, 533
Bst6I CTCTTC 1 cut(s) 1057
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 585
BstDEI CTNAG 5 cut(s) 97, 167, 225, 543, 1086
BstDSI CCRYGG 2 cut(s) 21, 52
BstF5I GGATG 4 cut(s) 69, 220, 557, 1193
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 469
BstKTI GATC 8 cut(s) 289, 383, 676, 755, 777, 1239, 1269, 1428
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
BstMWI GCNNNNNNNGC 5 cut(s) 413, 668, 911, 1087, 1199
BstNI CCWGG 1 cut(s) 763
BstNSI RCATGY 2 cut(s) 909, 1071
BstSCI CCNGG 1 cut(s) 761
BstSFI CTRYAG 2 cut(s) 710, 1354
BstSLI GKGCMC 1 cut(s) 1381
BstV1I GCAGC 3 cut(s) 629, 680, 1265
BstV2I GAAGAC 2 cut(s) 110, 1312
BstX2I RGATCY 1 cut(s) 380
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 1 cut(s) 380
BstZ17I GTATAC 1 cut(s) 1144
Bsu36I CCTNAGG 1 cut(s) 167
BsuRI GGCC 4 cut(s) 154, 556, 583, 852
BtgI CCRYGG 2 cut(s) 21, 52
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 4 cut(s) 69, 220, 557, 1193
BtsIMutI CAGTG 2 cut(s) 186, 1182
Cac8I GCNNGC 1 cut(s) 585
CciI TCATGA 3 cut(s) 144, 804, 864
CfoI GCGC 2 cut(s) 20, 469
Cfr10I RCCGGY 1 cut(s) 1398
Cfr13I GGNCC 3 cut(s) 196, 555, 1231
CseI GACGC 1 cut(s) 299
Csp6I GTAC 4 cut(s) 257, 304, 529, 597
CspAI ACCGGT 1 cut(s) 1398
CviQI GTAC 4 cut(s) 257, 304, 529, 597
DdeI CTNAG 5 cut(s) 97, 167, 225, 543, 1086
DinI GGCGCC 1 cut(s) 19
DpnI GATC 8 cut(s) 288, 382, 675, 754, 776, 1238, 1268, 1427
DpnII GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
EaeI YGGCCR 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 1057
EarI CTCTTC 1 cut(s) 1057
EciI GGCGGA 2 cut(s) 564, 1339
Eco130I CCWWGG 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 852
Eco47I GGWCC 2 cut(s) 196, 1231
Eco57I CTGAAG 2 cut(s) 573, 624
Eco81I CCTNAGG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 1231
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 761
EcoT14I CCWWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 862
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 21
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 3 cut(s) 407, 982, 1244
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 3 cut(s) 673, 774, 1266
FblI GTMKAC 1 cut(s) 1143
Fnu4HI GCNGC 3 cut(s) 618, 669, 1254
FokI GGATG 4 cut(s) 56, 227, 564, 1200
Fsp4HI GCNGC 3 cut(s) 618, 669, 1254
GlaI GCGC 2 cut(s) 19, 468
GluI GCNGC 3 cut(s) 618, 669, 1254
GsaI CCCAGC 2 cut(s) 394, 1111
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 4 cut(s) 154, 556, 583, 852
HapII CCGG 1 cut(s) 1399
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 20, 469
Hin1I GRCGYC 1 cut(s) 18
Hin6I GCGC 2 cut(s) 18, 467
HinP1I GCGC 2 cut(s) 18, 467
HinfI GANTC 4 cut(s) 43, 324, 1097, 1317
HpaII CCGG 1 cut(s) 1399
HphI GGTGA 4 cut(s) 23, 41, 175, 1235
Hpy166II GTNNAC 2 cut(s) 1144, 1180
Hpy188I TCNGA 6 cut(s) 100, 291, 298, 592, 888, 1044
Hpy8I GTNNAC 2 cut(s) 1144, 1180
HpyAV CCTTC 7 cut(s) 22, 457, 568, 599, 607, 842, 1151
HpyCH4III ACNGT 4 cut(s) 8, 53, 190, 533
HpyCH4IV ACGT 3 cut(s) 102, 396, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 413, 668, 911, 1087, 1199
HpyF3I CTNAG 5 cut(s) 97, 167, 225, 543, 1086
HpySE526I ACGT 3 cut(s) 102, 396, 634
Hsp92I GRCGYC 1 cut(s) 18
HspAI GCGC 2 cut(s) 18, 467
KasI GGCGCC 1 cut(s) 17
KflI GGGWCCC 1 cut(s) 1231
KpnI GGTACC 1 cut(s) 532
Ksp22I TGATCA 3 cut(s) 673, 774, 1266
Kzo9I GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
LguI GCTCTTC 1 cut(s) 1057
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 3 cut(s) 629, 680, 1265
LweI GCATC 4 cut(s) 676, 743, 892, 1255
MaeII ACGT 3 cut(s) 102, 396, 634
MaeIII GTNAC 4 cut(s) 61, 521, 533, 1343
MalI GATC 8 cut(s) 288, 382, 675, 754, 776, 1238, 1268, 1427
MboI GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
MboII GAAGA 6 cut(s) 38, 110, 362, 901, 1074, 1312
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 1 cut(s) 1381
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 6 cut(s) 292, 648, 869, 1206, 1270, 1420
MluNI TGGCCA 1 cut(s) 154
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 1 cut(s) 1326
MmeI TCCRAC 1 cut(s) 747
MnlI CCTC 6 cut(s) 50, 176, 205, 277, 719, 1132
Mox20I TGGCCA 1 cut(s) 154
Mph1103I ATGCAT 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 138
MscI TGGCCA 1 cut(s) 154
MseI TTAA 4 cut(s) 459, 693, 855, 1019
MslI CAYNNNNRTG 2 cut(s) 188, 191
Msp20I TGGCCA 1 cut(s) 154
MspA1I CMGCKG 3 cut(s) 602, 671, 785
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 1 cut(s) 1399
MspR9I CCNGG 1 cut(s) 763
Mva1269I GAATGC 2 cut(s) 833, 860
MvaI CCWGG 1 cut(s) 763
MwoI GCNNNNNNNGC 5 cut(s) 413, 668, 911, 1087, 1199
NarI GGCGCC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 739
NdeII GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
NlaIV GGNNCC 5 cut(s) 19, 135, 530, 1232, 1233
NmuCI GTSAC 1 cut(s) 533
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 2 cut(s) 909, 1071
OliI CACNNNNGTG 1 cut(s) 188
PagI TCATGA 3 cut(s) 144, 804, 864
PceI AGGCCT 1 cut(s) 852
PciSI GCTCTTC 1 cut(s) 1057
PctI GAATGC 2 cut(s) 833, 860
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 3 cut(s) 43, 324, 1097
PflMI CCANNNNNTGG 1 cut(s) 575
PinAI ACCGGT 1 cut(s) 1398
PkrI GCNGC 3 cut(s) 619, 670, 1255
PleI GAGTC 1 cut(s) 1325
PluTI GGCGCC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 1325
Ppu21I YACGTR 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 1231
Psp5II RGGWCCY 1 cut(s) 1231
Psp6I CCWGG 1 cut(s) 761
PspFI CCCAGC 2 cut(s) 390, 1107
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 5 cut(s) 19, 135, 530, 1232, 1233
PspPI GGNCC 3 cut(s) 196, 555, 1231
PspPPI RGGWCCY 1 cut(s) 1231
PsuI RGATCY 1 cut(s) 380
PvuII CAGCTG 3 cut(s) 602, 671, 785
RsaI GTAC 4 cut(s) 258, 305, 530, 598
RsaNI GTAC 4 cut(s) 257, 304, 529, 597
RseI CAYNNNNRTG 2 cut(s) 188, 191
SapI GCTCTTC 1 cut(s) 1057
SaqAI TTAA 4 cut(s) 459, 693, 855, 1019
SatI GCNGC 3 cut(s) 618, 669, 1254
Sau3AI GATC 8 cut(s) 286, 380, 673, 752, 774, 1236, 1266, 1425
Sau96I GGNCC 3 cut(s) 196, 555, 1231
SchI GAGTC 1 cut(s) 1326
ScrFI CCNGG 1 cut(s) 763
SduI GDGCHC 1 cut(s) 1381
SfaNI GCATC 4 cut(s) 676, 743, 892, 1255
SfcI CTRYAG 2 cut(s) 710, 1354
SfoI GGCGCC 1 cut(s) 19
SinI GGWCC 2 cut(s) 196, 1231
SmiMI CAYNNNNRTG 2 cut(s) 188, 191
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 6 cut(s) 292, 648, 869, 1206, 1270, 1420
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 2 cut(s) 549, 1350
SspDI GGCGCC 1 cut(s) 17
SspI AATATT 1 cut(s) 702
StuI AGGCCT 1 cut(s) 852
StyD4I CCNGG 1 cut(s) 761
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 4 cut(s) 8, 53, 190, 533
TaiI ACGT 3 cut(s) 105, 399, 637
TaqI TCGA 5 cut(s) 933, 1095, 1155, 1249, 1393
TasI AATT 6 cut(s) 292, 648, 869, 1206, 1270, 1420
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 3 cut(s) 43, 324, 1097
Tru1I TTAA 4 cut(s) 459, 693, 855, 1019
Tru9I TTAA 4 cut(s) 459, 693, 855, 1019
TscAI CASTG 2 cut(s) 193, 1189
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 3 cut(s) 617, 668, 1253
Tsp45I GTSAC 1 cut(s) 533
TspRI CASTG 2 cut(s) 193, 1189
Van91I CCANNNNNTGG 1 cut(s) 575
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 196, 1231
XapI RAATTY 2 cut(s) 292, 1420
XceI RCATGY 2 cut(s) 909, 1071
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XmiI GTMKAC 1 cut(s) 1143
XmnI GAANNNNTTC 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.