FvH4_4g12660

GPN-loop GTPase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008722
Physical Location & Seq
Forward (+)
16320249 .. 16323880
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12660.t2

Sequence Viewer

Length: 1215 bp
ATGGACATTGATTCCGATTTCCAGAAAATGAACATCGCGCCACAGCAACAACAAGTCGGAGCTTCATCGGTGCCAATGGATGCTGAGAAGGACGACTCTGCCGTCGTTAAAGGTAAAGAGACTGAGGACCTCAATGGTTCAATGGAGAAGCTAAATATTGAGGAGTCATCTTCCAGCTTCAAGAAGAAACCAGTTATTATCATTGTTGTTGGAATGGCAGGGAGTGGTAAAACAACATTTATGCATCGCTTGGTTGCGCACACTCATCAGAGGAATATTCGTGGTTATGTGATGAACCTCGACCCAGCTGTGCTGACTCTTCCATTTGGTGCAAATATAGATATCCGGGATACTGTTAAGTACAAGGAAGTTATGAAGCAGTTCAACCTTGGACCTAATGGTGGCATTTTGACCTCGCTCAACTTGTTTGCGACCAAGTTTGATGAGGTTATCAATGTTATCGAGAAGCGAGCAGATCAGCTTGATTATGTTCTTGTGGACACTCCTGGTCAAATTGAGATATTCACTTGGTCAGCATCTGGTGCTATCATCACTGAAGCATTTGCTTCAACATTTCCTACTGTTGTGACTTATGTAGTCGATACACCTCGCTCAGCTAGCCCAGTTACTTTTATGAGCAACATGCTTTATGCTTGTAGTATCCTATACAAGACACGGCTGCCCCTTGTGCTGGCATTCAACAAAACCGATGTGGCTCAACATGAATTTGCTTTGGAGTGGATGGAGGATTTTGAGACATTTCAAGCAGCAGTTAGTGAAGACCACTCATATACTTCAACTCTAACTGAGAGCCTCTCCCTTGTGTTGGATGAATTCTATAAGAACTTGCGGTCTGTTGGAGTGTCTGCGATTACTGGTGCTGGAATGGACGCCTTCTTTAAGGCCATTGAAGCAAGTGCTGAGGAATACATGGCCACCTATAAGGCTGATCTGGATCAGAGGCGTAAAGAGAAGGATCGTTTAGAGGAGGAACGCAGGAGGGAGACCATGGAGAAATTGAGAAAGGATATGGAGCAATCCAGGGGAGAAAATGTGGTCTTGAGCACTGGTTTGAAGGACAATGAGGAGAGGAGTAAAGCCATGATGGATGAGGATGAGGATGAGGAACTTGATGGGGATGAGGAGATAATAACTGAAGATGAGGATGTGATAGATGAAGATGAGGATGATGAAGATAGCAACTTCCCCTTTTAG

Protein Analysis

405

Amino Acids

45.49

Weight (kDa)

4.44

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 241 5.6e-80 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
Acc16I TGCGCA 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 70
AccII CGCG 1 cut(s) 38
AciI CCGC 1 cut(s) 850
AclWI GGATC 2 cut(s) 963, 984
AcoI YGGCCR 1 cut(s) 933
AcsI RAATTY 2 cut(s) 725, 833
AcuI CTGAAG 2 cut(s) 576, 1176
AcyI GRCGYC 1 cut(s) 891
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 401, 691, 826
AgsI TTSAA 9 cut(s) 141, 181, 385, 570, 700, 764, 798, 911, 1075
AjnI CCWGG 2 cut(s) 505, 1040
AluBI AGCT 6 cut(s) 62, 151, 177, 308, 481, 617
AluI AGCT 6 cut(s) 62, 151, 177, 308, 481, 617
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 3 cut(s) 113, 749, 998
AlwI GGATC 2 cut(s) 963, 984
AlwNI CAGNNNCTG 1 cut(s) 539
AoxI GGCC 2 cut(s) 903, 933
ApeKI GCWGC 2 cut(s) 679, 767
ApoI RAATTY 2 cut(s) 725, 833
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 40, 259
AspS9I GGNCC 2 cut(s) 127, 392
AsuC2I CCSGG 1 cut(s) 347
AsuNHI GCTAGC 1 cut(s) 617
AvaII GGWCC 2 cut(s) 127, 392
BalI TGGCCA 1 cut(s) 935
BanI GGYRCC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 1 cut(s) 1067
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 2 cut(s) 666, 779
BccI CCATC 3 cut(s) 736, 1099, 1127
BceAI ACGGC 2 cut(s) 86, 692
BciT130I CCWGG 2 cut(s) 507, 1042
BciVI GTATCC 2 cut(s) 343, 671
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 113, 749, 998
BfaI CTAG 1 cut(s) 618
BfuI GTATCC 2 cut(s) 343, 671
BisI GCNGC 2 cut(s) 680, 768
BlpI GCTNAGC 1 cut(s) 613
BlsI GCNGC 2 cut(s) 681, 769
Bme1390I CCNGG 3 cut(s) 347, 507, 1042
Bme18I GGWCC 2 cut(s) 127, 392
BmgT120I GGNCC 2 cut(s) 127, 392
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 3 cut(s) 347, 507, 1042
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 3 cut(s) 70, 253, 545
BmtI GCTAGC 1 cut(s) 621
BmuI ACTGGG 1 cut(s) 617
BpiI GAAGAC 1 cut(s) 786
BplI GAGNNNNNCTC 2 cut(s) 800, 832
Bpu10I CCTNAGC 1 cut(s) 921
Bpu1102I GCTNAGC 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 1081
BpuMI CCSGG 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 388, 1008, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 1038, 1068
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 691, 826
Bse1I ACTGG 4 cut(s) 191, 623, 880, 1072
Bse8I GATNNNNATC 1 cut(s) 954
BseBI CCWGG 2 cut(s) 507, 1042
BseDI CCNNGG 3 cut(s) 388, 1008, 1041
BseGI GGATG 9 cut(s) 85, 747, 835, 1114, 1120, 1126, 1144, 1171, 1192
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 3 cut(s) 401, 691, 826
BseMII CTCAG 5 cut(s) 75, 114, 627, 798, 912
BseNI ACTGG 4 cut(s) 191, 623, 880, 1072
BseRI GAGGAG 5 cut(s) 176, 1001, 1100, 1105, 1157
BseXI GCAGC 2 cut(s) 666, 779
BseYI CCCAGC 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 905, 935
BshNI GGYRCC 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 1067
BsiSI CCGG 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 401, 691, 826
BsmAI GTCTC 3 cut(s) 113, 749, 998
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 2 cut(s) 905, 935
Bso31I GGTCTC 1 cut(s) 998
Bsp1286I GDGCHC 1 cut(s) 1067
Bsp143I GATC 4 cut(s) 475, 949, 955, 976
Bsp1720I GCTNAGC 1 cut(s) 613
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 1 cut(s) 850
BspANI GGCC 2 cut(s) 905, 935
BspCNI CTCAG 5 cut(s) 76, 115, 626, 799, 913
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 72
BspOI GCTAGC 1 cut(s) 621
BspPI GGATC 2 cut(s) 963, 984
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 998
BsrI ACTGG 4 cut(s) 191, 623, 880, 1072
BssECI CCNNGG 3 cut(s) 388, 1008, 1041
BssMI GATC 4 cut(s) 475, 949, 955, 976
BssNI GRCGYC 1 cut(s) 891
BssT1I CCWWGG 2 cut(s) 388, 1008
Bst2UI CCWGG 2 cut(s) 507, 1042
Bst4CI ACNGT 2 cut(s) 355, 583
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 891
BstAPI GCANNNNNTGC 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 471, 619, 693
BstDEI CTNAG 5 cut(s) 84, 123, 613, 807, 921
BstDSI CCRYGG 1 cut(s) 1008
BstF5I GGATG 9 cut(s) 85, 747, 835, 1114, 1120, 1126, 1144, 1171, 1192
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 2 cut(s) 40, 259
BstKTI GATC 4 cut(s) 478, 952, 958, 979
BstMAI GTCTC 3 cut(s) 113, 749, 998
BstMBI GATC 4 cut(s) 475, 949, 955, 976
BstMWI GCNNNNNNNGC 4 cut(s) 542, 618, 688, 911
BstNI CCWGG 2 cut(s) 507, 1042
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 345, 505, 1040
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 666, 779
BstV2I GAAGAC 1 cut(s) 786
BsuI GTATCC 2 cut(s) 343, 671
BsuRI GGCC 2 cut(s) 905, 935
BtgI CCRYGG 1 cut(s) 1008
BtgZI GCGATG 2 cut(s) 19, 230
BtsCI GGATG 9 cut(s) 85, 747, 835, 1114, 1120, 1126, 1144, 1171, 1192
BtsIMutI CAGTG 2 cut(s) 552, 1065
Cac8I GCNNGC 3 cut(s) 471, 619, 693
CaiI CAGNNNCTG 1 cut(s) 539
CfoI GCGC 2 cut(s) 40, 259
Cfr13I GGNCC 2 cut(s) 127, 392
CseI GACGC 1 cut(s) 899
Csp6I GTAC 1 cut(s) 361
CviAII CATG 5 cut(s) 643, 722, 931, 1009, 1102
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 5 cut(s) 84, 123, 613, 807, 921
DpnI GATC 4 cut(s) 477, 951, 957, 978
DpnII GATC 4 cut(s) 475, 949, 955, 976
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 933
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 388, 1008
Eco31I GGTCTC 1 cut(s) 998
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 127, 392
Eco57I CTGAAG 2 cut(s) 576, 1176
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 2 cut(s) 505, 1040
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 388, 1008
EcoT22I ATGCAT 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 388, 1008
FaeI CATG 5 cut(s) 646, 725, 934, 1012, 1105
FatI CATG 5 cut(s) 642, 721, 930, 1008, 1101
Fnu4HI GCNGC 2 cut(s) 680, 768
FokI GGATG 9 cut(s) 92, 754, 842, 1121, 1127, 1133, 1151, 1178, 1199
Fsp4HI GCNGC 2 cut(s) 680, 768
FspBI CTAG 1 cut(s) 618
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 2 cut(s) 39, 258
GluI GCNGC 2 cut(s) 680, 768
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 905, 935
HapII CCGG 1 cut(s) 346
HgaI GACGC 1 cut(s) 899
HhaI GCGC 2 cut(s) 40, 259
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 5 cut(s) 646, 725, 934, 1012, 1105
Hin6I GCGC 2 cut(s) 38, 257
HinP1I GCGC 2 cut(s) 38, 257
HinfI GANTC 4 cut(s) 11, 95, 164, 316
HpaII CCGG 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 16, 59, 270, 960
Hpy188III TCNNGA 5 cut(s) 22, 181, 463, 953, 1060
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 82, 904, 967, 1069
HpyCH4III ACNGT 2 cut(s) 355, 583
HpyCH4V TGCA 2 cut(s) 244, 332
HpyF10VI GCNNNNNNNGC 4 cut(s) 542, 618, 688, 911
HpyF3I CTNAG 5 cut(s) 84, 123, 613, 807, 921
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 5 cut(s) 646, 725, 934, 1012, 1105
HspAI GCGC 2 cut(s) 38, 257
Kzo9I GATC 4 cut(s) 475, 949, 955, 976
LmnI GCTCC 2 cut(s) 59, 1033
Lsp1109I GCAGC 2 cut(s) 666, 779
LweI GCATC 3 cut(s) 70, 253, 545
MaeI CTAG 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 586, 625
MalI GATC 4 cut(s) 477, 951, 957, 978
MboI GATC 4 cut(s) 475, 949, 955, 976
MboII GAAGA 7 cut(s) 162, 196, 311, 791, 1169, 1190, 1205
MhlI GDGCHC 1 cut(s) 1067
MlsI TGGCCA 1 cut(s) 935
MluCI AATT 4 cut(s) 513, 725, 833, 1016
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 3 cut(s) 89, 173, 310
MmeI TCCRAC 4 cut(s) 37, 190, 807, 838
Mox20I TGGCCA 1 cut(s) 935
Mph1103I ATGCAT 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 935
MseI TTAA 3 cut(s) 108, 357, 900
Msp20I TGGCCA 1 cut(s) 935
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 3 cut(s) 347, 507, 1042
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 2 cut(s) 507, 1042
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 4 cut(s) 542, 618, 688, 911
NciI CCSGG 1 cut(s) 347
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 4 cut(s) 475, 949, 955, 976
NheI GCTAGC 1 cut(s) 617
NlaIII CATG 5 cut(s) 646, 725, 934, 1012, 1105
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 586
NsbI TGCGCA 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 246
NspI RCATGY 1 cut(s) 646
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 695
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 11
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 2 cut(s) 681, 769
PleI GAGTC 3 cut(s) 89, 172, 310
PpsI GAGTC 3 cut(s) 89, 172, 310
PpuMI RGGWCCY 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 505, 1040
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 2 cut(s) 505, 1040
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 127, 392
PspPPI RGGWCCY 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SaqAI TTAA 3 cut(s) 108, 357, 900
SatI GCNGC 2 cut(s) 680, 768
Sau3AI GATC 4 cut(s) 475, 949, 955, 976
Sau96I GGNCC 2 cut(s) 127, 392
SchI GAGTC 3 cut(s) 89, 173, 310
ScrFI CCNGG 3 cut(s) 347, 507, 1042
SduI GDGCHC 1 cut(s) 1067
SfaNI GCATC 3 cut(s) 70, 253, 545
SinI GGWCC 2 cut(s) 127, 392
SmlI CTYRAG 1 cut(s) 1060
SmoI CTYRAG 1 cut(s) 1060
Sse9I AATT 4 cut(s) 513, 725, 833, 1016
SsiI CCGC 1 cut(s) 850
SspI AATATT 2 cut(s) 157, 277
SspMI CTAG 1 cut(s) 618
StyD4I CCNGG 3 cut(s) 345, 505, 1040
StyI CCWWGG 2 cut(s) 388, 1008
TaaI ACNGT 2 cut(s) 355, 583
TaqI TCGA 3 cut(s) 300, 462, 600
TasI AATT 4 cut(s) 513, 725, 833, 1016
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 1 cut(s) 11
Tru1I TTAA 3 cut(s) 108, 357, 900
Tru9I TTAA 3 cut(s) 108, 357, 900
TscAI CASTG 2 cut(s) 559, 1072
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 679, 767
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 8 cut(s) 44, 54, 308, 389, 738, 846, 1191, 1206
TspRI CASTG 2 cut(s) 559, 1072
VpaK11BI GGWCC 2 cut(s) 127, 392
XapI RAATTY 2 cut(s) 725, 833
XceI RCATGY 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 618
Zsp2I ATGCAT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.