Rh4CG180200

GPN-loop GTPase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008722
Physical Location & Seq
Forward (+)
39107272 .. 39111026
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG180200.1

Sequence Viewer

Length: 924 bp
ATGAACCTTGACCCGGCTGTGTTGACTCTTCCATTCGGTGCAAACATTGACATCCGGGATACTGTTAAGTACAAGGAAGTGATGAAACAATTCAACCTTGGACCTAATGGTGGCATTTTGACCTCGCTTAATTTGTTTGCGACCAAGTTTGATGAGGTTATCAACGTTATCGAGAAGCGAGCAGATCAGCTTGATTATGTTCTTGTGGACACTCCTGGTCAGATTGAGATATTCACTTGGTCAGCATCTGGTGCTATCATCACAGAAGCATTTGCTTCAACATTTCCTACTGTTGTGGCTTATGTAGTCGATACACCTCGCTCAGCTAGCCCAGTTACTTTTATGAGCAACATGCTTTATGCTTGTAGTATCCTCTACAAGACAAGGCTCCCCCTTGTGCTGGCATTCAACAAAATTGACGTGGCTCAACATGAATTTGCTTTGGAGTGGATGCAGGATTTTGAGGCATTTCAAGCAGCAGTGAGTGAAGACCACTCATACACTTCAACTTTAACAGAGAGCCTCTCCCTTGTATTGGATGAATTCTATAAGAACTTGCGGTCTGTTGGAGTGTCTGCTATTTCTGGTGCTGGAATGGATGCCTTCTTTAAGGCTATTGAAGCAAGTGCTGAGGAGTACATGGCCACCTATAAGGCTGATCTGGACAAGAGACGCGCAGAAAAGGAGCGTTTAGAGGAGGAACATAGGAGGGAGAACATGGAGAAGTTGAGGAAGGATATGGAGCAATCCAGGGGAGAAAATGTGGTCTTGAGCACTGGTTTGAAGGACAATGGAGAGAGGAGCAAAGCCATGATGGATGAGGATGAGGATGAGGAACTGGACGAGGATGAGGAGATAATAACTGAAGATGAGGATGTCATAGATGAGGATGAAGATGATGAAGATAGCAAGTTCCCCTTTTAG

Protein Analysis

307

Amino Acids

34.74

Weight (kDa)

4.29

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 2 - 210 2.3e-69 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 675
AciI CCGC 1 cut(s) 559
AclI AACGTT 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 2 cut(s) 434, 542
AcuI CTGAAG 1 cut(s) 885
AfaI GTAC 2 cut(s) 71, 638
AfiI CCNNNNNNNGG 4 cut(s) 13, 110, 400, 535
AgsI TTSAA 7 cut(s) 94, 279, 409, 473, 507, 620, 784
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 214, 749
AluBI AGCT 2 cut(s) 190, 326
AluI AGCT 2 cut(s) 190, 326
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 1 cut(s) 664
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 1 cut(s) 642
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 2 cut(s) 434, 542
Asp700I GAANNNNTTC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 2 cut(s) 14, 56
AsuNHI GCTAGC 1 cut(s) 326
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 776
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 488
BccI CCATC 1 cut(s) 808
BciT130I CCWGG 2 cut(s) 216, 751
BciVI GTATCC 2 cut(s) 52, 380
BcnI CCSGG 2 cut(s) 14, 56
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 1 cut(s) 327
BfuI GTATCC 2 cut(s) 52, 380
BisI GCNGC 1 cut(s) 477
BlpI GCTNAGC 1 cut(s) 322
BlsI GCNGC 1 cut(s) 478
Bme1390I CCNGG 4 cut(s) 14, 56, 216, 751
Bme18I GGWCC 1 cut(s) 101
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 4 cut(s) 14, 56, 216, 751
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 3 cut(s) 254, 441, 589
BmtI GCTAGC 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 495
BplI GAGNNNNNCTC 2 cut(s) 509, 541
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 2 cut(s) 14, 56
BsaJI CCNNGG 2 cut(s) 97, 750
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 110, 400, 535
Bse1I ACTGG 3 cut(s) 332, 781, 843
BseBI CCWGG 2 cut(s) 216, 751
BseDI CCNNGG 2 cut(s) 97, 750
BseLI CCNNNNNNNGG 4 cut(s) 13, 110, 400, 535
BseMII CTCAG 2 cut(s) 336, 621
BseNI ACTGG 3 cut(s) 332, 781, 843
BseRI GAGGAG 4 cut(s) 647, 710, 814, 866
BseXI GCAGC 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 776
BsiSI CCGG 2 cut(s) 14, 55
BslI CCNNNNNNNGG 4 cut(s) 13, 110, 400, 535
BsmAI GTCTC 1 cut(s) 664
BsmBI CGTCTC 1 cut(s) 664
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 2 cut(s) 184, 658
Bsp1720I GCTNAGC 1 cut(s) 322
BspACI CCGC 1 cut(s) 559
BspANI GGCC 1 cut(s) 644
BspCNI CTCAG 2 cut(s) 335, 622
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 1 cut(s) 389
BspOI GCTAGC 1 cut(s) 330
BsrI ACTGG 3 cut(s) 332, 781, 843
BssECI CCNNGG 2 cut(s) 97, 750
BssMI GATC 2 cut(s) 184, 658
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 216, 751
Bst4CI ACNGT 2 cut(s) 64, 292
Bst6I CTCTTC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 180, 328, 402
BstDEI CTNAG 2 cut(s) 322, 630
BstFNI CGCG 1 cut(s) 675
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 2 cut(s) 187, 661
BstMAI GTCTC 1 cut(s) 664
BstMBI GATC 2 cut(s) 184, 658
BstMWI GCNNNNNNNGC 4 cut(s) 251, 327, 473, 620
BstNI CCWGG 2 cut(s) 216, 751
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 4 cut(s) 12, 54, 214, 749
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 1 cut(s) 488
BstV2I GAAGAC 1 cut(s) 495
BsuI GTATCC 2 cut(s) 52, 380
BsuRI GGCC 1 cut(s) 644
BtrI CACGTC 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 486, 774
Cac8I GCNNGC 3 cut(s) 180, 328, 402
CaiI CAGNNNCTG 1 cut(s) 248
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 681
Csp6I GTAC 2 cut(s) 70, 637
CviAII CATG 5 cut(s) 352, 431, 640, 718, 811
CviQI GTAC 2 cut(s) 70, 637
DdeI CTNAG 2 cut(s) 322, 630
DpnI GATC 2 cut(s) 186, 660
DpnII GATC 2 cut(s) 184, 658
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 885
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 214, 749
EcoT14I CCWWGG 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 664
FaeI CATG 5 cut(s) 355, 434, 643, 721, 814
FalI AAGNNNNNCTT 1 cut(s) 902
FatI CATG 5 cut(s) 351, 430, 639, 717, 810
Fnu4HI GCNGC 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 477
FspBI CTAG 1 cut(s) 327
GlaI GCGC 1 cut(s) 676
GluI GCNGC 1 cut(s) 477
HaeIII GGCC 1 cut(s) 644
HapII CCGG 2 cut(s) 14, 55
HgaI GACGC 1 cut(s) 681
HhaI GCGC 1 cut(s) 677
Hin1II CATG 5 cut(s) 355, 434, 643, 721, 814
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 1 cut(s) 25
HpaII CCGG 2 cut(s) 14, 55
Hpy166II GTNNAC 2 cut(s) 24, 208
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 3 cut(s) 172, 662, 769
Hpy8I GTNNAC 2 cut(s) 24, 208
HpyAV CCTTC 3 cut(s) 613, 727, 778
HpyCH4III ACNGT 2 cut(s) 64, 292
HpyCH4IV ACGT 2 cut(s) 165, 420
HpyCH4V TGCA 2 cut(s) 41, 454
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 327, 473, 620
HpyF3I CTNAG 2 cut(s) 322, 630
HpySE526I ACGT 2 cut(s) 165, 420
Hsp92II CATG 5 cut(s) 355, 434, 643, 721, 814
HspAI GCGC 1 cut(s) 675
Kzo9I GATC 2 cut(s) 184, 658
LmnI GCTCC 4 cut(s) 393, 685, 742, 801
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 3 cut(s) 254, 441, 589
MaeI CTAG 1 cut(s) 327
MaeII ACGT 2 cut(s) 165, 420
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 186, 660
MboI GATC 2 cut(s) 184, 658
MboII GAAGA 5 cut(s) 20, 500, 878, 905, 914
MhlI GDGCHC 1 cut(s) 776
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 5 cut(s) 89, 130, 414, 434, 542
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 1 cut(s) 19
MmeI TCCRAC 1 cut(s) 547
Mox20I TGGCCA 1 cut(s) 644
MroXI GAANNNNTTC 1 cut(s) 89
MscI TGGCCA 1 cut(s) 644
MseI TTAA 4 cut(s) 66, 129, 512, 609
Msp20I TGGCCA 1 cut(s) 644
MspI CCGG 2 cut(s) 14, 55
MspR9I CCNGG 4 cut(s) 14, 56, 216, 751
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 2 cut(s) 216, 751
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 4 cut(s) 251, 327, 473, 620
NciI CCSGG 2 cut(s) 14, 56
NdeII GATC 2 cut(s) 184, 658
NheI GCTAGC 1 cut(s) 326
NlaIII CATG 5 cut(s) 355, 434, 643, 721, 814
NlaIV GGNNCC 1 cut(s) 389
NspI RCATGY 1 cut(s) 355
PctI GAATGC 1 cut(s) 404
PdmI GAANNNNTTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 54
PkrI GCNGC 1 cut(s) 478
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 2 cut(s) 214, 749
PspGI CCWGG 2 cut(s) 214, 749
PspN4I GGNNCC 1 cut(s) 389
PspPI GGNCC 1 cut(s) 101
PstNI CAGNNNCTG 1 cut(s) 248
RsaI GTAC 2 cut(s) 71, 638
RsaNI GTAC 2 cut(s) 70, 637
SaqAI TTAA 4 cut(s) 66, 129, 512, 609
SatI GCNGC 1 cut(s) 477
Sau3AI GATC 2 cut(s) 184, 658
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 4 cut(s) 14, 56, 216, 751
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 3 cut(s) 254, 441, 589
SinI GGWCC 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
Sse9I AATT 5 cut(s) 89, 130, 414, 434, 542
SsiI CCGC 1 cut(s) 559
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 4 cut(s) 12, 54, 214, 749
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 2 cut(s) 64, 292
TaiI ACGT 2 cut(s) 168, 423
TaqI TCGA 2 cut(s) 171, 309
TasI AATT 5 cut(s) 89, 130, 414, 434, 542
TatI WGTACW 2 cut(s) 69, 636
Tru1I TTAA 4 cut(s) 66, 129, 512, 609
Tru9I TTAA 4 cut(s) 66, 129, 512, 609
TscAI CASTG 2 cut(s) 486, 781
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 6 cut(s) 17, 98, 447, 555, 906, 915
TspRI CASTG 2 cut(s) 486, 781
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 2 cut(s) 434, 542
XceI RCATGY 1 cut(s) 355
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.