Rw4G014020

GPN-loop GTPase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008722
Physical Location & Seq
Forward (+)
32729376 .. 32732734
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014020.1

Sequence Viewer

Length: 1077 bp
ATGCCGCCAGACGCAACCACAAGTGAACAGGCTGAGGACCTCCATGGTTCGATGGAGAAACTTAATATTGAGGAATCATCCACCAGCTTCAAGAAAAAACCTGTTATTATTATTGTTGTGGGCATGGCAGGGAGTGGTAAAACAACCTTTCTTCATCGTTTGGTTGCCCACACTCATCAATCCGGTATTCGTGGCTATGTGATGAACCTTGACCCGGCTGTGTTGACTCTTCCATTCGGTGCAAACATTGACATCCGGGATACTGTTAAGTACAAGGAAGTGATGAAACAATTCAACCTTGGACCTAATGGTGGCATTTTGACCTCGCTCAATTTGTTTGCGACCAAGTTTGATGAGGTTATCAACGTTATCGAGAAGCGAGCAGATCAGCTTGATTATGTTCTTGTGGACACTCCTGGTCAGATTGAGATATTCACTTGGTCAGCATCTGGTGCTATCATCACAGAAGCATTTGCTTCAACATTTCCTACTGTTGTGACTTATGTAGTCGATACACCTCGCTCAGCTAGCCCAACAAGGCTCCCCCTTGTGCTGGCATTCAATAAAATTGACGTGGCTCAACATGAATTTGCTTTGGAGTGGATGCAGGATTTTGAGGCATTTCAAGCAGCAGTGAGTGAAGACCACTCATACACTTCAACCTTAACAGAGAGCCTCTCCCTTGTGTTGGATGAATTCTATAAGAACTTGCGGTCTGTTGGAGTGTCTGCTATTTCTGGTGCTGGAATGGACGCCTTCTTTAAGGCTATTGAAGCAAGTGCTGAGGAGTACATGGCCACCTATAAGGCTGATCTGGACAAGAGACGTGCAGAAAAGGAGCGTTTAGAGGAGGAACATAGGAGGGAGAACATGGAGAAGTTGAGGAAGGATATGGAGCAATCCAGGGGAGAAAATGTGGTCTTGAGCACTGGTTTGAAGGACAATGGAGAGAGGAGCAAAGCCATGATGGATGAGGATGAGGATGAGGAACTGGACGAGGATGAGGAGATAATAACTGAAGATGAGGATGTCATAGATGAGGATGAAGATGATGAAGATAGCAAGTTCCCCTTTTAG

Protein Analysis

358

Amino Acids

40.15

Weight (kDa)

4.46

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 39 - 261 6.7e-68 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 712
AclI AACGTT 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 2 cut(s) 587, 695
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 753
AfaI GTAC 2 cut(s) 272, 791
AfiI CCNNNNNNNGG 4 cut(s) 214, 311, 553, 688
AgsI TTSAA 8 cut(s) 91, 295, 480, 562, 626, 660, 773, 937
AjiI CACGTC 2 cut(s) 574, 827
AjnI CCWGG 2 cut(s) 415, 902
AluBI AGCT 3 cut(s) 87, 391, 527
AluI AGCT 3 cut(s) 87, 391, 527
Alw21I GWGCWC 1 cut(s) 929
Alw26I GTCTC 1 cut(s) 817
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 1 cut(s) 795
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 2 cut(s) 587, 695
Asp700I GAANNNNTTC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 37, 302
AsuC2I CCSGG 2 cut(s) 215, 257
AsuNHI GCTAGC 1 cut(s) 527
AvaII GGWCC 2 cut(s) 37, 302
BalI TGGCCA 1 cut(s) 797
BbsI GAAGAC 1 cut(s) 648
Bbv12I GWGCWC 1 cut(s) 929
BbvCI CCTCAGC 2 cut(s) 33, 783
BbvI GCAGC 1 cut(s) 641
BccI CCATC 2 cut(s) 46, 961
BciT130I CCWGG 2 cut(s) 417, 904
BciVI GTATCC 1 cut(s) 253
BcnI CCSGG 2 cut(s) 215, 257
BcoDI GTCTC 1 cut(s) 817
BfaI CTAG 1 cut(s) 528
BfuI GTATCC 1 cut(s) 253
BisI GCNGC 2 cut(s) 5, 630
BlpI GCTNAGC 1 cut(s) 523
BlsI GCNGC 2 cut(s) 6, 631
Bme1390I CCNGG 4 cut(s) 215, 257, 417, 904
Bme18I GGWCC 2 cut(s) 37, 302
BmgBI CACGTC 2 cut(s) 574, 827
BmgT120I GGNCC 2 cut(s) 37, 302
BmiI GGNNCC 1 cut(s) 542
BmrFI CCNGG 4 cut(s) 215, 257, 417, 904
BmsI GCATC 2 cut(s) 455, 594
BmtI GCTAGC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 648
BplI GAGNNNNNCTC 2 cut(s) 662, 694
Bpu10I CCTNAGC 2 cut(s) 33, 783
Bpu1102I GCTNAGC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 943
BpuMI CCSGG 2 cut(s) 215, 257
BsaHI GRCGYC 1 cut(s) 753
BsaJI CCNNGG 3 cut(s) 43, 298, 903
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 900, 930
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 311, 553, 688
Bse1I ACTGG 2 cut(s) 934, 996
BseBI CCWGG 2 cut(s) 417, 904
BseDI CCNNGG 3 cut(s) 43, 298, 903
BseLI CCNNNNNNNGG 4 cut(s) 214, 311, 553, 688
BseMII CTCAG 3 cut(s) 24, 537, 774
BseNI ACTGG 2 cut(s) 934, 996
BseRI GAGGAG 4 cut(s) 800, 863, 967, 1019
BseXI GCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 849
BshFI GGCC 1 cut(s) 797
BsiHKAI GWGCWC 1 cut(s) 929
BsiSI CCGG 3 cut(s) 183, 215, 256
BslI CCNNNNNNNGG 4 cut(s) 214, 311, 553, 688
BsmAI GTCTC 1 cut(s) 817
BsmBI CGTCTC 1 cut(s) 817
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 929
Bsp143I GATC 2 cut(s) 385, 811
Bsp1720I GCTNAGC 1 cut(s) 523
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 2 cut(s) 5, 712
BspANI GGCC 1 cut(s) 797
BspCNI CTCAG 3 cut(s) 25, 536, 775
BspLI GGNNCC 1 cut(s) 542
BspOI GCTAGC 1 cut(s) 531
BsrI ACTGG 2 cut(s) 934, 996
BssECI CCNNGG 3 cut(s) 43, 298, 903
BssMI GATC 2 cut(s) 385, 811
BssNI GRCGYC 1 cut(s) 753
BssT1I CCWWGG 2 cut(s) 43, 298
Bst2UI CCWGG 2 cut(s) 417, 904
Bst4CI ACNGT 2 cut(s) 265, 493
Bst6I CTCTTC 1 cut(s) 234
BstACI GRCGYC 1 cut(s) 753
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 381, 529, 555
BstDEI CTNAG 3 cut(s) 33, 523, 783
BstDSI CCRYGG 1 cut(s) 43
BstKTI GATC 2 cut(s) 388, 814
BstMAI GTCTC 1 cut(s) 817
BstMBI GATC 2 cut(s) 385, 811
BstMWI GCNNNNNNNGC 4 cut(s) 452, 528, 626, 773
BstNI CCWGG 2 cut(s) 417, 904
BstSCI CCNGG 4 cut(s) 213, 255, 415, 902
BstV1I GCAGC 1 cut(s) 641
BstV2I GAAGAC 1 cut(s) 648
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 1 cut(s) 797
BtgI CCRYGG 1 cut(s) 43
BtrI CACGTC 2 cut(s) 574, 827
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 2 cut(s) 639, 927
Cac8I GCNNGC 3 cut(s) 381, 529, 555
CaiI CAGNNNCTG 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 37, 302
CseI GACGC 2 cut(s) 20, 761
Csp6I GTAC 2 cut(s) 271, 790
CviAII CATG 6 cut(s) 44, 124, 584, 793, 871, 964
CviQI GTAC 2 cut(s) 271, 790
DdeI CTNAG 3 cut(s) 33, 523, 783
DpnI GATC 2 cut(s) 387, 813
DpnII GATC 2 cut(s) 385, 811
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 43, 298
Eco47I GGWCC 2 cut(s) 37, 302
Eco57I CTGAAG 1 cut(s) 1038
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 415, 902
EcoT14I CCWWGG 2 cut(s) 43, 298
ErhI CCWWGG 2 cut(s) 43, 298
Esp3I CGTCTC 1 cut(s) 817
FaeI CATG 6 cut(s) 47, 127, 587, 796, 874, 967
FalI AAGNNNNNCTT 1 cut(s) 1055
FatI CATG 6 cut(s) 43, 123, 583, 792, 870, 963
Fnu4HI GCNGC 2 cut(s) 5, 630
Fsp4HI GCNGC 2 cut(s) 5, 630
FspBI CTAG 1 cut(s) 528
GluI GCNGC 2 cut(s) 5, 630
HaeIII GGCC 1 cut(s) 797
HapII CCGG 3 cut(s) 183, 215, 256
HgaI GACGC 2 cut(s) 20, 761
Hin1I GRCGYC 1 cut(s) 753
Hin1II CATG 6 cut(s) 47, 127, 587, 796, 874, 967
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 2 cut(s) 74, 226
HpaII CCGG 3 cut(s) 183, 215, 256
Hpy166II GTNNAC 3 cut(s) 26, 225, 409
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 4 cut(s) 91, 373, 815, 922
Hpy8I GTNNAC 3 cut(s) 26, 225, 409
HpyAV CCTTC 3 cut(s) 766, 880, 931
HpyCH4III ACNGT 2 cut(s) 265, 493
HpyCH4IV ACGT 3 cut(s) 366, 573, 826
HpyCH4V TGCA 3 cut(s) 242, 607, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 452, 528, 626, 773
HpyF3I CTNAG 3 cut(s) 33, 523, 783
HpySE526I ACGT 3 cut(s) 366, 573, 826
Hsp92I GRCGYC 1 cut(s) 753
Hsp92II CATG 6 cut(s) 47, 127, 587, 796, 874, 967
Kzo9I GATC 2 cut(s) 385, 811
LmnI GCTCC 4 cut(s) 546, 838, 895, 954
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 2 cut(s) 455, 594
MaeI CTAG 1 cut(s) 528
MaeII ACGT 3 cut(s) 366, 573, 826
MaeIII GTNAC 1 cut(s) 496
MalI GATC 2 cut(s) 387, 813
MboI GATC 2 cut(s) 385, 811
MboII GAAGA 6 cut(s) 143, 221, 653, 1031, 1058, 1067
MhlI GDGCHC 1 cut(s) 929
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 5 cut(s) 290, 331, 567, 587, 695
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 2 cut(s) 669, 700
Mox20I TGGCCA 1 cut(s) 797
MroXI GAANNNNTTC 1 cut(s) 290
MscI TGGCCA 1 cut(s) 797
MseI TTAA 4 cut(s) 63, 267, 665, 762
Msp20I TGGCCA 1 cut(s) 797
MspI CCGG 3 cut(s) 183, 215, 256
MspR9I CCNGG 4 cut(s) 215, 257, 417, 904
Mva1269I GAATGC 1 cut(s) 557
MvaI CCWGG 2 cut(s) 417, 904
MwoI GCNNNNNNNGC 4 cut(s) 452, 528, 626, 773
NciI CCSGG 2 cut(s) 215, 257
NcoI CCATGG 1 cut(s) 43
NdeII GATC 2 cut(s) 385, 811
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 6 cut(s) 47, 127, 587, 796, 874, 967
NlaIV GGNNCC 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 496
PctI GAATGC 1 cut(s) 557
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 2 cut(s) 6, 631
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 37
Psp1406I AACGTT 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 2 cut(s) 415, 902
PspGI CCWGG 2 cut(s) 415, 902
PspN4I GGNNCC 1 cut(s) 542
PspPI GGNCC 2 cut(s) 37, 302
PspPPI RGGWCCY 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 449
RsaI GTAC 2 cut(s) 272, 791
RsaNI GTAC 2 cut(s) 271, 790
SaqAI TTAA 4 cut(s) 63, 267, 665, 762
SatI GCNGC 2 cut(s) 5, 630
Sau3AI GATC 2 cut(s) 385, 811
Sau96I GGNCC 2 cut(s) 37, 302
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 4 cut(s) 215, 257, 417, 904
SduI GDGCHC 1 cut(s) 929
SfaNI GCATC 2 cut(s) 455, 594
SinI GGWCC 2 cut(s) 37, 302
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
Sse9I AATT 5 cut(s) 290, 331, 567, 587, 695
SsiI CCGC 2 cut(s) 5, 712
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 4 cut(s) 213, 255, 415, 902
StyI CCWWGG 2 cut(s) 43, 298
TaaI ACNGT 2 cut(s) 265, 493
TaiI ACGT 3 cut(s) 369, 576, 829
TaqI TCGA 3 cut(s) 50, 372, 510
TasI AATT 5 cut(s) 290, 331, 567, 587, 695
TatI WGTACW 2 cut(s) 270, 789
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 4 cut(s) 63, 267, 665, 762
Tru9I TTAA 4 cut(s) 63, 267, 665, 762
TscAI CASTG 2 cut(s) 639, 934
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 7 cut(s) 143, 218, 299, 600, 708, 1059, 1068
TspRI CASTG 2 cut(s) 639, 934
VpaK11BI GGWCC 2 cut(s) 37, 302
XapI RAATTY 2 cut(s) 587, 695
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.