Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0008711

Physical Location & Seq

Genomic Coordinates Forward (+)

16856653 .. 16858178

Gene Structure 1526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g13260.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 780 bp

ATGATGGCTAATCAGAATCTGAACGGGCGGGTAGCAATCGTGACCGGCGGTTCGCGTGGCATCGGCCGTGAAATAGTACTCCACCTTCACTCCCTGGGCGCAAGAGTTGTCATCAACTACGCCTCAAGTTCAAACCAGGCAGACGAACTGGCCTCCCAACTCAACACCAAGACCTCACCACAGGTATCAGCCATTGCTGCTAAAGCAGATGTCTCAGACCCAGATCAGGTAAAGCTCCTGTTCGACAAGGCCGAGCAAGAAATGGGTGCCCAGGTCCACATCCTGGTCAACTGCGCCGGCGTCTTGGATCCCAAGTACCCTTCTCTGGCCGACACCACTGTCGACGATTGGGACAACACCTTCAACATCAACACCAAAGGAGCATTCTTGATTTGCAGAGAGGCCACCAACAGACTCGCACGCGGCGCCGGCGGAAGAATCATCATGATCACCACCTCCGTCGTTGGGGGACTGATGCCTGGTTACGCCGCCTATGCTGCTTCCAAGGCCGCTGTGGAAACCATGACCAAAATAGTCGCCAAGGAGCTCAAGGGTAGCGGGATCACTGCCAACTGTGTTGCTCCGGGCCCTGTCGCCACCGAACTCTTCTTCGCCGGGAAAAGTGAGGAAACAGTTAACAGGATCACGGAAGCTTGCCCTCTCGGCCGCCTGGGTCAGCCCCACGATGTGTCCCAGGTTGTTGGGTTCTTGGCTGGAGATGCAGGGGAGTGGATCAATGGTCAGGTCCTTCGGGTTAATGGTGGAGCTCTCATCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212

Pfam Domains

Protein Families

adh_short_C2

Protein Analysis

260

Amino Acids

27.09

Weight (kDa)

6.09

Isoelectric Point (pI)

23.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SDR	PF23441	3 - 258	3.3e-09	SDR-like rossmann domain
adh_short	PF00106	10 - 203	1.2e-48	short chain dehydrogenase
KR	PF08659	13 - 179	6.8e-15	KR domain
adh_short_C2	PF13561	19 - 255	9.7e-56	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008711)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13180
fragaria_vesca	FvH4_4g13260
malus_domestica	MD03G1214200.v1.1 MD10G1132600.v1.1 MD14G1002100.v1.1
prunus_persica	Prupe.8G053700_v2.0.a1 Prupe.8G053800_v2.0.a1
pyrus_communis	pycom03g16290 pycom10g11380 pycom49g00170
rosa_chinensis	RchiOBHm_Chr3g0497441
rosa_laevigata	RLG00000022461 RLG00000022463
rosa_multiflora	Rmu_co8233681.1_g000001 Rmu_sc0008086.1_g000011
rosa_roxburghii	Rroxscaffold_166G00440680 Rroxscaffold_6G00388040
rosa_rugosa	Rorug03G0293000
rosa_samantha	Rh3AG336600 Rh3BG374900 Rh3CG370800 Rh3DG371700
rosa_wichuraiana	Rw3G029590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	338
AccB1I	GGYRCC	2 cut(s)	266, 425
AccB7I	CCANNNNNTGG	1 cut(s)	283
AccI	GTMKAC	1 cut(s)	342
AccII	CGCG	2 cut(s)	55, 423
AciI	CCGC	8 cut(s)	28, 48, 423, 432, 489, 510, 558, 667
AclWI	GGATC	5 cut(s)	302, 315, 569, 650, 740
AcoI	YGGCCR	3 cut(s)	64, 327, 664
AcyI	GRCGYC	2 cut(s)	300, 426
AdeI	CACNNNGTG	1 cut(s)	688
AfaI	GTAC	2 cut(s)	78, 317
AfiI	CCNNNNNNNGG	4 cut(s)	226, 283, 325, 465
AgsI	TTSAA	2 cut(s)	132, 364
AjnI	CCWGG	7 cut(s)	93, 135, 270, 282, 478, 669, 693
AjuI	GAANNNNNNNTTGG	2 cut(s)	368, 400
AluBI	AGCT	4 cut(s)	235, 547, 653, 767
AluI	AGCT	4 cut(s)	235, 547, 653, 767
Alw21I	GWGCWC	2 cut(s)	549, 769
Alw26I	GTCTC	1 cut(s)	217
AlwI	GGATC	5 cut(s)	302, 315, 569, 650, 740
AlwNI	CAGNNNCTG	1 cut(s)	19
AoxI	GGCC	8 cut(s)	64, 150, 249, 327, 402, 507, 586, 664
ApaI	GGGCCC	1 cut(s)	590
ApeKI	GCWGC	2 cut(s)	197, 497
ArsI	GACNNNNNNTTYG	2 cut(s)	34, 66
AspLEI	GCGC	3 cut(s)	101, 296, 428
AspS9I	GGNCC	4 cut(s)	274, 586, 587, 745
AsuC2I	CCSGG	2 cut(s)	585, 616
AsuHPI	GGTGA	2 cut(s)	168, 442
AvaII	GGWCC	2 cut(s)	274, 745
BaeGI	GKGCMC	2 cut(s)	271, 590
BamHI	GGATCC	1 cut(s)	307
BanI	GGYRCC	2 cut(s)	266, 425
BanII	GRGCYC	3 cut(s)	549, 590, 769
BarI	GAAGNNNNNNTAC	2 cut(s)	69, 101
Bbv12I	GWGCWC	2 cut(s)	549, 769
BbvI	GCAGC	2 cut(s)	184, 484
BceAI	ACGGC	1 cut(s)	51
BciT130I	CCWGG	7 cut(s)	95, 137, 272, 284, 480, 671, 695
BclI	TGATCA	1 cut(s)	447
BcnI	CCSGG	2 cut(s)	585, 616
BcoDI	GTCTC	1 cut(s)	217
BfoI	RGCGCY	1 cut(s)	429
BglI	GCCNNNNNGGC	1 cut(s)	663
BisI	GCNGC	6 cut(s)	198, 424, 489, 498, 510, 667
BlsI	GCNGC	6 cut(s)	199, 425, 490, 499, 511, 668
BmcAI	AGTACT	1 cut(s)	78
Bme1390I	CCNGG	9 cut(s)	95, 137, 272, 284, 480, 585, 616, 671, 695
Bme18I	GGWCC	2 cut(s)	274, 745
BmgT120I	GGNCC	4 cut(s)	274, 586, 587, 745
BmiI	GGNNCC	4 cut(s)	268, 309, 427, 588
BmrFI	CCNGG	9 cut(s)	95, 137, 272, 284, 480, 585, 616, 671, 695
BmsI	GCATC	3 cut(s)	69, 465, 709
BpmI	CTGGAG	1 cut(s)	735
BpuEI	CTTGAG	2 cut(s)	109, 533
BpuMI	CCSGG	2 cut(s)	585, 616
BsaHI	GRCGYC	2 cut(s)	300, 426
BsaJI	CCNNGG	7 cut(s)	93, 94, 270, 504, 540, 670, 693
BsaXI	ACNNNNNCTCC	2 cut(s)	74, 104
Bsc4I	CCNNNNNNNGG	4 cut(s)	226, 283, 325, 465
Bse118I	RCCGGY	3 cut(s)	44, 296, 428
Bse1I	ACTGG	1 cut(s)	153
Bse3DI	GCAATG	1 cut(s)	192
BseBI	CCWGG	7 cut(s)	95, 137, 272, 284, 480, 671, 695
BseDI	CCNNGG	7 cut(s)	93, 94, 270, 504, 540, 670, 693
BseGI	GGATG	1 cut(s)	279
BseLI	CCNNNNNNNGG	4 cut(s)	226, 283, 325, 465
BseMI	GCAATG	1 cut(s)	192
BseMII	CTCAG	1 cut(s)	228
BseNI	ACTGG	1 cut(s)	153
BseSI	GKGCMC	2 cut(s)	271, 590
BseX3I	CGGCCG	2 cut(s)	64, 664
BseXI	GCAGC	2 cut(s)	184, 484
Bsh1236I	CGCG	2 cut(s)	55, 423
Bsh1285I	CGRYCG	2 cut(s)	67, 667
BshFI	GGCC	8 cut(s)	66, 152, 251, 329, 404, 509, 588, 666
BshNI	GGYRCC	2 cut(s)	266, 425
BsiEI	CGRYCG	2 cut(s)	67, 667
BsiHKAI	GWGCWC	2 cut(s)	549, 769
BsiSI	CCGG	5 cut(s)	45, 297, 429, 584, 615
BslFI	GGGAC	3 cut(s)	365, 483, 676
BslI	CCNNNNNNNGG	4 cut(s)	226, 283, 325, 465
BsmAI	GTCTC	1 cut(s)	217
BsmFI	GGGAC	3 cut(s)	365, 483, 676
BsmI	GAATGC	1 cut(s)	383
BsnI	GGCC	8 cut(s)	66, 152, 251, 329, 404, 509, 588, 666
Bsp120I	GGGCCC	1 cut(s)	586
Bsp1286I	GDGCHC	4 cut(s)	271, 549, 590, 769
Bsp143I	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
BspACI	CCGC	8 cut(s)	28, 48, 423, 432, 489, 510, 558, 667
BspANI	GGCC	8 cut(s)	66, 152, 251, 329, 404, 509, 588, 666
BspCNI	CTCAG	1 cut(s)	227
BspFNI	CGCG	2 cut(s)	55, 423
BspHI	TCATGA	1 cut(s)	444
BspLI	GGNNCC	4 cut(s)	268, 309, 427, 588
BspPI	GGATC	5 cut(s)	302, 315, 569, 650, 740
BspT107I	GGYRCC	2 cut(s)	266, 425
BsrDI	GCAATG	1 cut(s)	192
BsrFI	RCCGGY	3 cut(s)	44, 296, 428
BsrI	ACTGG	1 cut(s)	153
BssAI	RCCGGY	3 cut(s)	44, 296, 428
BssECI	CCNNGG	7 cut(s)	93, 94, 270, 504, 540, 670, 693
BssMI	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
BssNI	GRCGYC	2 cut(s)	300, 426
BssT1I	CCWWGG	2 cut(s)	504, 540
Bst2UI	CCWGG	7 cut(s)	95, 137, 272, 284, 480, 671, 695
Bst4CI	ACNGT	3 cut(s)	340, 575, 634
Bst6I	CTCTTC	1 cut(s)	611
BstACI	GRCGYC	2 cut(s)	300, 426
BstC8I	GCNNGC	4 cut(s)	298, 421, 430, 655
BstDEI	CTNAG	1 cut(s)	214
BstF5I	GGATG	1 cut(s)	279
BstFNI	CGCG	2 cut(s)	55, 423
BstH2I	RGCGCY	1 cut(s)	429
BstHHI	GCGC	3 cut(s)	101, 296, 428
BstKTI	GATC	6 cut(s)	226, 310, 450, 564, 645, 735
BstMAI	GTCTC	1 cut(s)	217
BstMBI	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
BstMCI	CGRYCG	2 cut(s)	67, 667
BstMWI	GCNNNNNNNGC	9 cut(s)	197, 203, 425, 429, 494, 497, 506, 663, 719
BstNI	CCWGG	7 cut(s)	95, 137, 272, 284, 480, 671, 695
BstSCI	CCNGG	9 cut(s)	93, 135, 270, 282, 478, 583, 614, 669, 693
BstSLI	GKGCMC	2 cut(s)	271, 590
BstUI	CGCG	2 cut(s)	55, 423
BstV1I	GCAGC	2 cut(s)	184, 484
BstX2I	RGATCY	1 cut(s)	307
BstXI	CCANNNNNNTGG	1 cut(s)	701
BstYI	RGATCY	1 cut(s)	307
BstZI	CGGCCG	2 cut(s)	64, 664
BsuRI	GGCC	8 cut(s)	66, 152, 251, 329, 404, 509, 588, 666
BtsCI	GGATG	1 cut(s)	279
BtsI	GCAGTG	1 cut(s)	564
BtsIMutI	CAGTG	2 cut(s)	336, 564
Cac8I	GCNNGC	4 cut(s)	298, 421, 430, 655
CaiI	CAGNNNCTG	1 cut(s)	19
CciI	TCATGA	1 cut(s)	444
CfoI	GCGC	3 cut(s)	101, 296, 428
Cfr10I	RCCGGY	3 cut(s)	44, 296, 428
Cfr13I	GGNCC	4 cut(s)	274, 586, 587, 745
CseI	GACGC	1 cut(s)	289
Csp6I	GTAC	2 cut(s)	77, 316
CviAII	CATG	2 cut(s)	445, 523
CviQI	GTAC	2 cut(s)	77, 316
DdeI	CTNAG	1 cut(s)	214
DinI	GGCGCC	1 cut(s)	427
DpnI	GATC	6 cut(s)	225, 309, 449, 563, 644, 734
DpnII	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
DraIII	CACNNNGTG	1 cut(s)	688
DrdI	GACNNNNNNGTC	1 cut(s)	338
DseDI	GACNNNNNNGTC	1 cut(s)	338
EaeI	YGGCCR	3 cut(s)	64, 327, 664
EagI	CGGCCG	2 cut(s)	64, 664
Eam1104I	CTCTTC	1 cut(s)	611
EarI	CTCTTC	1 cut(s)	611
EciI	GGCGGA	1 cut(s)	447
Ecl136II	GAGCTC	2 cut(s)	547, 767
EclXI	CGGCCG	2 cut(s)	64, 664
Eco130I	CCWWGG	2 cut(s)	504, 540
Eco24I	GRGCYC	3 cut(s)	549, 590, 769
Eco47I	GGWCC	2 cut(s)	274, 745
Eco52I	CGGCCG	2 cut(s)	64, 664
Eco53kI	GAGCTC	2 cut(s)	547, 767
EcoICRI	GAGCTC	2 cut(s)	547, 767
EcoO109I	RGGNCCY	2 cut(s)	587, 745
EcoRII	CCWGG	7 cut(s)	93, 135, 270, 282, 478, 669, 693
EcoT14I	CCWWGG	2 cut(s)	504, 540
EcoT38I	GRGCYC	3 cut(s)	549, 590, 769
EgeI	GGCGCC	1 cut(s)	427
EheI	GGCGCC	1 cut(s)	427
ErhI	CCWWGG	2 cut(s)	504, 540
FaeI	CATG	2 cut(s)	448, 526
FaiI	YATR	3 cut(s)	446, 495, 524
FaqI	GGGAC	3 cut(s)	365, 483, 676
FatI	CATG	2 cut(s)	444, 522
FauI	CCCGC	2 cut(s)	21, 551
FbaI	TGATCA	1 cut(s)	447
FblI	GTMKAC	1 cut(s)	342
Fnu4HI	GCNGC	6 cut(s)	198, 424, 489, 498, 510, 667
FokI	GGATG	1 cut(s)	266
FriOI	GRGCYC	3 cut(s)	549, 590, 769
Fsp4HI	GCNGC	6 cut(s)	198, 424, 489, 498, 510, 667
GlaI	GCGC	3 cut(s)	100, 295, 427
GluI	GCNGC	6 cut(s)	198, 424, 489, 498, 510, 667
GsuI	CTGGAG	1 cut(s)	735
HaeII	RGCGCY	1 cut(s)	429
HaeIII	GGCC	8 cut(s)	66, 152, 251, 329, 404, 509, 588, 666
HapII	CCGG	5 cut(s)	45, 297, 429, 584, 615
HgaI	GACGC	1 cut(s)	289
HhaI	GCGC	3 cut(s)	101, 296, 428
Hin1I	GRCGYC	2 cut(s)	300, 426
Hin1II	CATG	2 cut(s)	448, 526
Hin6I	GCGC	3 cut(s)	99, 294, 426
HinP1I	GCGC	3 cut(s)	99, 294, 426
HincII	GTYRAC	3 cut(s)	289, 343, 637
HindII	GTYRAC	3 cut(s)	289, 343, 637
HindIII	AAGCTT	1 cut(s)	651
HinfI	GANTC	3 cut(s)	16, 414, 438
HpaI	GTTAAC	1 cut(s)	637
HpaII	CCGG	5 cut(s)	45, 297, 429, 584, 615
HphI	GGTGA	2 cut(s)	168, 442
Hpy166II	GTNNAC	4 cut(s)	277, 289, 343, 637
Hpy188I	TCNGA	4 cut(s)	15, 21, 217, 779
Hpy188III	TCNNGA	3 cut(s)	40, 388, 445
Hpy8I	GTNNAC	4 cut(s)	277, 289, 343, 637
Hpy99I	CGWCG	2 cut(s)	347, 464
HpyAV	CCTTC	4 cut(s)	95, 330, 370, 758
HpyCH4III	ACNGT	3 cut(s)	340, 575, 634
HpyCH4V	TGCA	2 cut(s)	396, 722
HpyF10VI	GCNNNNNNNGC	9 cut(s)	197, 203, 425, 429, 494, 497, 506, 663, 719
HpyF3I	CTNAG	1 cut(s)	214
Hsp92I	GRCGYC	2 cut(s)	300, 426
Hsp92II	CATG	2 cut(s)	448, 526
HspAI	GCGC	3 cut(s)	99, 294, 426
KasI	GGCGCC	1 cut(s)	425
KroI	GCCGGC	2 cut(s)	296, 428
KroNI	GCCGGC	2 cut(s)	298, 430
Ksp22I	TGATCA	1 cut(s)	447
KspAI	GTTAAC	1 cut(s)	637
Kzo9I	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
LmnI	GCTCC	5 cut(s)	240, 380, 544, 586, 764
Lsp1109I	GCAGC	2 cut(s)	184, 484
LweI	GCATC	3 cut(s)	69, 465, 709
MaeIII	GTNAC	2 cut(s)	40, 482
MalI	GATC	6 cut(s)	225, 309, 449, 563, 644, 734
MboI	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
MboII	GAAGA	3 cut(s)	447, 598, 601
MflI	RGATCY	1 cut(s)	307
MhlI	GDGCHC	4 cut(s)	271, 549, 590, 769
Mly113I	GGCGCC	1 cut(s)	426
MlyI	GAGTC	1 cut(s)	408
MnlI	CCTC	7 cut(s)	133, 163, 184, 394, 466, 619, 669
MreI	CGCCGGCG	2 cut(s)	296, 428
MroNI	GCCGGC	2 cut(s)	296, 428
MseI	TTAA	2 cut(s)	636, 756
MspA1I	CMGCKG	1 cut(s)	512
MspI	CCGG	5 cut(s)	45, 297, 429, 584, 615
MspR9I	CCNGG	9 cut(s)	95, 137, 272, 284, 480, 585, 616, 671, 695
Mva1269I	GAATGC	1 cut(s)	383
MvaI	CCWGG	7 cut(s)	95, 137, 272, 284, 480, 671, 695
MvnI	CGCG	2 cut(s)	55, 423
MwoI	GCNNNNNNNGC	9 cut(s)	197, 203, 425, 429, 494, 497, 506, 663, 719
NaeI	GCCGGC	2 cut(s)	298, 430
NarI	GGCGCC	1 cut(s)	426
NciI	CCSGG	2 cut(s)	585, 616
NdeII	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
NgoMIV	GCCGGC	2 cut(s)	296, 428
NlaIII	CATG	2 cut(s)	448, 526
NlaIV	GGNNCC	4 cut(s)	268, 309, 427, 588
NmeAIII	GCCGAG	2 cut(s)	277, 642
NmuCI	GTSAC	1 cut(s)	40
PagI	TCATGA	1 cut(s)	444
PasI	CCCWGGG	1 cut(s)	94
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PctI	GAATGC	1 cut(s)	383
PdiI	GCCGGC	2 cut(s)	298, 430
PfeI	GAWTC	2 cut(s)	16, 438
PflMI	CCANNNNNTGG	1 cut(s)	283
PkrI	GCNGC	6 cut(s)	199, 425, 490, 499, 511, 668
PleI	GAGTC	1 cut(s)	408
PluTI	GGCGCC	1 cut(s)	429
PpsI	GAGTC	1 cut(s)	408
PpuMI	RGGWCCY	1 cut(s)	745
Psp124BI	GAGCTC	2 cut(s)	549, 769
Psp5II	RGGWCCY	1 cut(s)	745
Psp6I	CCWGG	7 cut(s)	93, 135, 270, 282, 478, 669, 693
PspGI	CCWGG	7 cut(s)	93, 135, 270, 282, 478, 669, 693
PspN4I	GGNNCC	4 cut(s)	268, 309, 427, 588
PspOMI	GGGCCC	1 cut(s)	586
PspPI	GGNCC	4 cut(s)	274, 586, 587, 745
PspPPI	RGGWCCY	1 cut(s)	745
PstNI	CAGNNNCTG	1 cut(s)	19
PsuI	RGATCY	1 cut(s)	307
RsaI	GTAC	2 cut(s)	78, 317
RsaNI	GTAC	2 cut(s)	77, 316
SacI	GAGCTC	2 cut(s)	549, 769
SalI	GTCGAC	1 cut(s)	341
SaqAI	TTAA	2 cut(s)	636, 756
SatI	GCNGC	6 cut(s)	198, 424, 489, 498, 510, 667
Sau3AI	GATC	6 cut(s)	223, 307, 447, 561, 642, 732
Sau96I	GGNCC	4 cut(s)	274, 586, 587, 745
ScaI	AGTACT	1 cut(s)	78
SchI	GAGTC	1 cut(s)	408
ScrFI	CCNGG	9 cut(s)	95, 137, 272, 284, 480, 585, 616, 671, 695
SduI	GDGCHC	4 cut(s)	271, 549, 590, 769
SfaNI	GCATC	3 cut(s)	69, 465, 709
SfoI	GGCGCC	1 cut(s)	427
SgrAI	CRCCGGYG	2 cut(s)	296, 428
SinI	GGWCC	2 cut(s)	274, 745
SmlI	CTYRAG	2 cut(s)	124, 548
SmoI	CTYRAG	2 cut(s)	124, 548
SsiI	CCGC	8 cut(s)	28, 48, 423, 432, 489, 510, 558, 667
SspDI	GGCGCC	1 cut(s)	425
SstI	GAGCTC	2 cut(s)	549, 769
StyD4I	CCNGG	9 cut(s)	93, 135, 270, 282, 478, 583, 614, 669, 693
StyI	CCWWGG	2 cut(s)	504, 540
TaaI	ACNGT	3 cut(s)	340, 575, 634
TaqI	TCGA	2 cut(s)	243, 342
TatI	WGTACW	1 cut(s)	76
TauI	GCSGC	4 cut(s)	426, 491, 512, 669
TfiI	GAWTC	2 cut(s)	16, 438
Tru1I	TTAA	2 cut(s)	636, 756
Tru9I	TTAA	2 cut(s)	636, 756
TscAI	CASTG	2 cut(s)	343, 571
TseFI	GTSAC	1 cut(s)	40
TseI	GCWGC	2 cut(s)	197, 497
Tsp45I	GTSAC	1 cut(s)	40
TspGWI	ACGGA	2 cut(s)	448, 662
TspRI	CASTG	2 cut(s)	343, 571
Van91I	CCANNNNNTGG	1 cut(s)	283
VpaK11BI	GGWCC	2 cut(s)	274, 745
XcmI	CCANNNNNNNNNTGG	1 cut(s)	511
XmiI	GTMKAC	1 cut(s)	342
ZrmI	AGTACT	1 cut(s)	78

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.