pycom10g11380

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008711
Physical Location & Seq
Forward (+)
14938586 .. 14939368
783 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g11380.1

Sequence Viewer

Length: 783 bp
ATGGCTACTCAAACAACCCTTCCCCTCAACGGTCGCGTAGCAATCGTGACTGGCGGTTCGCGCGGAATTGGCCGCGCCATCGCAACCCAACTCCACTCTGCCGGTGCAAGGGTCGTGATCAATTATGCTTCATCAAACTCAACACAAGCAGATCAACTAGTCTCTGAGCTGAATCACGAGACTACCCAACTGCGTGCCATTGCAGTTCGAGCAGACGTTTCGGACCCCGATCAGGTAAAGCAACTATTTGAAAAATCGGAAGAAGAATTCGGGAGCCAAGTCCACATCGTTGTGAACTGCGCAGGAGTTTTGGATCCAAAGTATCCCAGTTTGGCCAACACGACAGTGGAAGAATGGGACAACACATTCAACGTGAACACCAAAGGCGCGTTCTTAGTTTCTAGAGAAGCGGCCAAGAGGCTGGCACGTGGTGGCGGCGGGAGAATAATCTTGATAACGACGTCCCTTGTAGGGGCGCTTATGCCGGGGTACGCAGCTTATGCGGCATCCAAGGCGGCGGTGGAGACGATGGCAAAGATTTTGGCTAAGGAGCTCAAGGGCACCGGAATAACTGTGAATTGCGTTGCGCCGGGGCCTGTGGCGACGGAGTTGTTCTTTGCCGGCAAAAGTGAGGAGACGATTAAGAGGATTGAGGATGCTTCACCTCTGGGTCGATTAGGTAAGCCCAAGGATGTATCTCAGCTTGTAGGGTTTTTGGCGGGAGATGCTGGGGAGTTGGTTAATGGCCAGGTCATTAGGGTCAATGGTGGATTTGTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

261

Amino Acids

27.26

Weight (kDa)

7.76

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 205 7.7e-50 short chain dehydrogenase
SDR PF23441 14 - 258 9.9e-11 SDR-like rossmann domain
KR PF08659 15 - 193 1.1e-19 KR domain
Epimerase PF01370 15 - 220 2.1e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 21 - 258 1.5e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 464
Acc16I TGCGCA 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 560
AccII CGCG 5 cut(s) 36, 61, 63, 75, 389
AclWI GGATC 2 cut(s) 308, 321
AcoI YGGCCR 4 cut(s) 70, 333, 411, 745
AcsI RAATTY 1 cut(s) 266
AcvI CACGTG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 461
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 5 cut(s) 29, 108, 232, 471, 472
AgsI TTSAA 2 cut(s) 251, 370
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 1 cut(s) 747
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 4 cut(s) 169, 497, 553, 703
AluI AGCT 4 cut(s) 169, 497, 553, 703
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 4 cut(s) 166, 173, 518, 629
AlwI GGATC 2 cut(s) 308, 321
AoxI GGCC 5 cut(s) 70, 333, 411, 593, 745
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspLEI GCGC 6 cut(s) 63, 77, 302, 389, 478, 589
AspS9I GGNCC 2 cut(s) 223, 593
AsuC2I CCSGG 2 cut(s) 486, 591
AsuHPI GGTGA 1 cut(s) 654
AvaII GGWCC 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 563
BalI TGGCCA 2 cut(s) 335, 747
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 555
BauI CACGAG 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 1 cut(s) 506
BccI CCATC 2 cut(s) 86, 523
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciT130I CCWGG 1 cut(s) 749
BciVI GTATCC 1 cut(s) 333
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 486, 591
BcoDI GTCTC 4 cut(s) 166, 173, 518, 629
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 2 cut(s) 158, 402
BfoI RGCGCY 1 cut(s) 479
BfuI GTATCC 1 cut(s) 333
BisI GCNGC 6 cut(s) 73, 411, 436, 495, 504, 516
BlsI GCNGC 6 cut(s) 74, 412, 437, 496, 505, 517
Bme1390I CCNGG 3 cut(s) 486, 591, 749
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 223, 593
BmiI GGNNCC 5 cut(s) 225, 275, 315, 562, 594
BmrFI CCNGG 3 cut(s) 486, 591, 749
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 3 cut(s) 515, 646, 715
BmuI ACTGGG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 2 cut(s) 486, 591
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 461
BsaJI CCNNGG 4 cut(s) 485, 510, 590, 687
BsaWI WCCGGW 1 cut(s) 563
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 108, 232, 471, 472
Bse118I RCCGGY 2 cut(s) 101, 620
Bse1I ACTGG 2 cut(s) 55, 327
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 749
BseDI CCNNGG 4 cut(s) 485, 510, 590, 687
BseGI GGATG 3 cut(s) 506, 661, 697
BseLI CCNNNNNNNGG 5 cut(s) 29, 108, 232, 471, 472
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 156, 713
BseNI ACTGG 2 cut(s) 55, 327
BseRI GAGGAG 1 cut(s) 647
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 1 cut(s) 506
BseYI CCCAGC 1 cut(s) 728
Bsh1236I CGCG 5 cut(s) 36, 61, 63, 75, 389
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 5 cut(s) 72, 335, 413, 595, 747
BshNI GGYRCC 1 cut(s) 560
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 5 cut(s) 102, 485, 564, 590, 621
BslFI GGGAC 2 cut(s) 371, 448
BslI CCNNNNNNNGG 5 cut(s) 29, 108, 232, 471, 472
BsmAI GTCTC 4 cut(s) 166, 173, 518, 629
BsmBI CGTCTC 2 cut(s) 518, 629
BsmFI GGGAC 2 cut(s) 371, 448
BsnI GGCC 5 cut(s) 72, 335, 413, 595, 747
Bsp1286I GDGCHC 2 cut(s) 555, 563
Bsp143I GATC 4 cut(s) 117, 151, 229, 313
BspANI GGCC 5 cut(s) 72, 335, 413, 595, 747
BspCNI CTCAG 2 cut(s) 157, 712
BspFNI CGCG 5 cut(s) 36, 61, 63, 75, 389
BspLI GGNNCC 5 cut(s) 225, 275, 315, 562, 594
BspPI GGATC 2 cut(s) 308, 321
BspT107I GGYRCC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 101, 620
BsrI ACTGG 2 cut(s) 55, 327
BssAI RCCGGY 2 cut(s) 101, 620
BssECI CCNNGG 4 cut(s) 485, 510, 590, 687
BssMI GATC 4 cut(s) 117, 151, 229, 313
BssNI GRCGYC 1 cut(s) 461
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 510, 687
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 749
Bst4CI ACNGT 3 cut(s) 32, 346, 574
BstACI GRCGYC 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 195, 423, 622
BstDEI CTNAG 4 cut(s) 165, 394, 546, 699
BstF5I GGATG 3 cut(s) 506, 661, 697
BstFNI CGCG 5 cut(s) 36, 61, 63, 75, 389
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 6 cut(s) 63, 77, 302, 389, 478, 589
BstKTI GATC 4 cut(s) 120, 154, 232, 316
BstMAI GTCTC 4 cut(s) 166, 173, 518, 629
BstMBI GATC 4 cut(s) 117, 151, 229, 313
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 7 cut(s) 60, 69, 209, 500, 503, 512, 725
BstNI CCWGG 1 cut(s) 749
BstSCI CCNGG 3 cut(s) 484, 589, 747
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 5 cut(s) 36, 61, 63, 75, 389
BstV1I GCAGC 1 cut(s) 506
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 5 cut(s) 72, 335, 413, 595, 747
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 506, 661, 697
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 3 cut(s) 195, 423, 622
CfoI GCGC 6 cut(s) 63, 77, 302, 389, 478, 589
Cfr10I RCCGGY 2 cut(s) 101, 620
Cfr13I GGNCC 2 cut(s) 223, 593
Csp6I GTAC 1 cut(s) 490
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 4 cut(s) 165, 394, 546, 699
DpnI GATC 4 cut(s) 119, 153, 231, 315
DpnII GATC 4 cut(s) 117, 151, 229, 313
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 4 cut(s) 70, 333, 411, 745
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 2 cut(s) 510, 687
Eco24I GRGCYC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 553
Eco72I CACGTG 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 553
EcoO109I RGGNCCY 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 747
EcoT14I CCWWGG 2 cut(s) 510, 687
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 2 cut(s) 510, 687
Esp3I CGTCTC 2 cut(s) 518, 629
FaiI YATR 5 cut(s) 126, 482, 501, 779, 781
FaqI GGGAC 2 cut(s) 371, 448
FauI CCCGC 2 cut(s) 431, 712
FauNDI CATATG 1 cut(s) 779
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 6 cut(s) 73, 411, 436, 495, 504, 516
FokI GGATG 3 cut(s) 493, 668, 704
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 6 cut(s) 73, 411, 436, 495, 504, 516
FspBI CTAG 2 cut(s) 158, 402
FspI TGCGCA 1 cut(s) 301
GlaI GCGC 6 cut(s) 62, 76, 301, 388, 477, 588
GluI GCNGC 6 cut(s) 73, 411, 436, 495, 504, 516
GsaI CCCAGC 1 cut(s) 732
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 5 cut(s) 72, 335, 413, 595, 747
HapII CCGG 5 cut(s) 102, 485, 564, 590, 621
HhaI GCGC 6 cut(s) 63, 77, 302, 389, 478, 589
Hin1I GRCGYC 1 cut(s) 461
Hin6I GCGC 6 cut(s) 61, 75, 300, 387, 476, 587
HinP1I GCGC 6 cut(s) 61, 75, 300, 387, 476, 587
HinfI GANTC 1 cut(s) 172
HpaII CCGG 5 cut(s) 102, 485, 564, 590, 621
HphI GGTGA 1 cut(s) 654
Hpy166II GTNNAC 3 cut(s) 283, 295, 376
Hpy188I TCNGA 3 cut(s) 166, 223, 259
Hpy188III TCNNGA 6 cut(s) 46, 115, 176, 271, 402, 451
Hpy8I GTNNAC 3 cut(s) 283, 295, 376
Hpy99I CGWCG 2 cut(s) 463, 607
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 3 cut(s) 32, 346, 574
HpyCH4IV ACGT 4 cut(s) 216, 372, 427, 461
HpyCH4V TGCA 2 cut(s) 107, 203
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 69, 209, 500, 503, 512, 725
HpyF3I CTNAG 4 cut(s) 165, 394, 546, 699
HpySE526I ACGT 4 cut(s) 216, 372, 427, 461
Hsp92I GRCGYC 1 cut(s) 461
HspAI GCGC 6 cut(s) 61, 75, 300, 387, 476, 587
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 4 cut(s) 117, 151, 229, 313
LmnI GCTCC 2 cut(s) 273, 550
Lsp1109I GCAGC 1 cut(s) 506
LweI GCATC 3 cut(s) 515, 646, 715
MaeI CTAG 2 cut(s) 158, 402
MaeII ACGT 4 cut(s) 216, 372, 427, 461
MaeIII GTNAC 1 cut(s) 46
MalI GATC 4 cut(s) 119, 153, 231, 315
MboI GATC 4 cut(s) 117, 151, 229, 313
MboII GAAGA 3 cut(s) 272, 275, 362
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 555, 563
MlsI TGGCCA 2 cut(s) 335, 747
MluCI AATT 4 cut(s) 66, 121, 266, 577
MluNI TGGCCA 2 cut(s) 335, 747
MnlI CCTC 6 cut(s) 35, 411, 625, 639, 646, 675
Mox20I TGGCCA 2 cut(s) 335, 747
MroNI GCCGGC 1 cut(s) 620
MscI TGGCCA 2 cut(s) 335, 747
MseI TTAA 2 cut(s) 642, 741
MslI CAYNNNNRTG 2 cut(s) 290, 344
Msp20I TGGCCA 2 cut(s) 335, 747
MspI CCGG 5 cut(s) 102, 485, 564, 590, 621
MspR9I CCNGG 3 cut(s) 486, 591, 749
MvaI CCWGG 1 cut(s) 749
MvnI CGCG 5 cut(s) 36, 61, 63, 75, 389
MwoI GCNNNNNNNGC 7 cut(s) 60, 69, 209, 500, 503, 512, 725
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 2 cut(s) 486, 591
NdeI CATATG 1 cut(s) 779
NdeII GATC 4 cut(s) 117, 151, 229, 313
NgoMIV GCCGGC 1 cut(s) 620
NlaIV GGNNCC 5 cut(s) 225, 275, 315, 562, 594
NmuCI GTSAC 1 cut(s) 46
NsbI TGCGCA 1 cut(s) 301
OliI CACNNNNGTG 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 172
PkrI GCNGC 6 cut(s) 74, 412, 437, 496, 505, 517
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 428
Psp124BI GAGCTC 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 747
PspCI CACGTG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 728
PspGI CCWGG 1 cut(s) 747
PspN4I GGNNCC 5 cut(s) 225, 275, 315, 562, 594
PspPI GGNCC 2 cut(s) 223, 593
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 2 cut(s) 290, 344
SacI GAGCTC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 642, 741
SatI GCNGC 6 cut(s) 73, 411, 436, 495, 504, 516
Sau3AI GATC 4 cut(s) 117, 151, 229, 313
Sau96I GGNCC 2 cut(s) 223, 593
ScrFI CCNGG 3 cut(s) 486, 591, 749
SduI GDGCHC 2 cut(s) 555, 563
SfaNI GCATC 3 cut(s) 515, 646, 715
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 290, 344
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 4 cut(s) 66, 121, 266, 577
SspMI CTAG 2 cut(s) 158, 402
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 3 cut(s) 484, 589, 747
StyI CCWWGG 2 cut(s) 510, 687
TaaI ACNGT 3 cut(s) 32, 346, 574
TaiI ACGT 4 cut(s) 219, 375, 430, 464
TaqI TCGA 2 cut(s) 208, 673
TasI AATT 4 cut(s) 66, 121, 266, 577
TauI GCSGC 5 cut(s) 75, 413, 438, 506, 518
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 2 cut(s) 642, 741
Tru9I TTAA 2 cut(s) 642, 741
TscAI CASTG 1 cut(s) 351
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 1 cut(s) 351
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 1 cut(s) 266
XbaI TCTAGA 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 2 cut(s) 343, 517
XspI CTAG 2 cut(s) 158, 402
ZraI GACGTC 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.