RLG00000022461

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008711
Physical Location & Seq
Forward (+)
781036 .. 783718
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022461

Sequence Viewer

Length: 651 bp
ATGGATAATCAGAATCTGAATGGTCGGGTGGCAATTGTCACCGGTGGTTCTCGTGGCATCGGCCGTGAAATTGTTGTCCACCTCCACTCCCTAGGCGCAAGACTTGTCATCAACTACGCTTCCAGTTCCAAGCAGGCAGACGAATTGGCCTCCCACCTCAACAGCAACAACAACAAGAGCTCACAGTTGGCAGCCATTGCAGTAAAAGCAAATGTTTCATATCCAGATCAGATGAAACTCCTCTTCGACAAAGCCGAGCAAGAATTGGGTGCCCAGGTCCAGATCCTGGTCAACTGCGCCGGCGTCTTGGATCCCAAGTACCCGTCTTTGGCCGACACCAGTGTCCAGGATTGGGATAACATATTCAATGTCAACACCAAAGGAGCCTTCTTGGCTTGTAGAGAAGCCGCCAACAGACTCCCGTTCACCGCCTATATTGCCTCCAAGGCCGCCATGGAGACCATGACCAAAATTGTCGCCAAGGAGCTCAAGGGCACCGGCATCACTGCCAACTATGTTGCTCCCGGCCCTGTTGCCATCGACCTCTTCTTCGCTGGTAAAAGTGAGGAGACCATCAACAGGATCCAAGATGCTTGCCCTCTTGGCCACCTTGGTCGGCCCCAGATGTTTCCCAGGTTGTCGAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

217

Amino Acids

23.28

Weight (kDa)

8.42

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 9 - 139 2.7e-24 short chain dehydrogenase
KR PF08659 12 - 131 1.5e-10 KR domain
adh_short_C2 PF13561 18 - 138 3.8e-17 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 142 - 181 2.7e-07 short chain dehydrogenase
adh_short_C2 PF13561 143 - 208 1.2e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 269, 494
AccB7I CCANNNNNTGG 1 cut(s) 286
AciI CCGC 3 cut(s) 408, 429, 450
AclWI GGATC 5 cut(s) 277, 305, 318, 577, 590
AcoI YGGCCR 3 cut(s) 61, 330, 604
AcsI RAATTY 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 4 cut(s) 286, 328, 352, 579
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 4 cut(s) 273, 285, 345, 632
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 2 cut(s) 180, 487
AluI AGCT 2 cut(s) 180, 487
Alw21I GWGCWC 2 cut(s) 182, 489
Alw26I GTCTC 2 cut(s) 452, 563
AlwI GGATC 5 cut(s) 277, 305, 318, 577, 590
AlwNI CAGNNNCTG 2 cut(s) 16, 286
AoxI GGCC 7 cut(s) 61, 147, 330, 447, 526, 604, 617
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 643
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AsiGI ACCGGT 1 cut(s) 41
AspA2I CCTAGG 1 cut(s) 91
AspLEI GCGC 2 cut(s) 98, 299
AspS9I GGNCC 3 cut(s) 277, 527, 618
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 31, 418
AvaII GGWCC 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 91
BaeGI GKGCMC 2 cut(s) 274, 497
BalI TGGCCA 1 cut(s) 606
BamHI GGATCC 2 cut(s) 310, 582
BanI GGYRCC 2 cut(s) 269, 494
BanII GRGCYC 2 cut(s) 182, 489
BauI CACGAG 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 182, 489
BbvI GCAGC 1 cut(s) 203
BccI CCATC 2 cut(s) 545, 581
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 4 cut(s) 275, 287, 347, 634
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 452, 563
BfaI CTAG 1 cut(s) 92
BglI GCCNNNNNGGC 3 cut(s) 392, 446, 603
BisI GCNGC 3 cut(s) 192, 408, 450
BlnI CCTAGG 1 cut(s) 91
BlsI GCNGC 3 cut(s) 193, 409, 451
Bme1390I CCNGG 5 cut(s) 275, 287, 347, 525, 634
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 3 cut(s) 277, 527, 618
BmiI GGNNCC 6 cut(s) 271, 312, 385, 496, 584, 620
BmrFI CCNGG 5 cut(s) 275, 287, 347, 525, 634
BmsI GCATC 3 cut(s) 66, 510, 580
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 2 cut(s) 452, 563
BsaJI CCNNGG 7 cut(s) 91, 273, 444, 453, 480, 610, 632
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 328, 352, 579
Bse118I RCCGGY 3 cut(s) 41, 299, 497
Bse1I ACTGG 2 cut(s) 123, 339
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 4 cut(s) 275, 287, 347, 634
BseDI CCNNGG 7 cut(s) 91, 273, 444, 453, 480, 610, 632
BseLI CCNNNNNNNGG 4 cut(s) 286, 328, 352, 579
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 123, 339
BseRI GAGGAG 2 cut(s) 230, 581
BseSI GKGCMC 2 cut(s) 274, 497
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 7 cut(s) 63, 149, 332, 449, 528, 606, 619
BshNI GGYRCC 2 cut(s) 269, 494
BshTI ACCGGT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 2 cut(s) 182, 489
BsiSI CCGG 4 cut(s) 42, 300, 498, 525
BslI CCNNNNNNNGG 4 cut(s) 286, 328, 352, 579
BsmAI GTCTC 2 cut(s) 452, 563
BsnI GGCC 7 cut(s) 63, 149, 332, 449, 528, 606, 619
Bso31I GGTCTC 2 cut(s) 452, 563
Bsp1286I GDGCHC 4 cut(s) 182, 274, 489, 497
Bsp143I GATC 4 cut(s) 226, 282, 310, 582
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 3 cut(s) 408, 429, 450
BspANI GGCC 7 cut(s) 63, 149, 332, 449, 528, 606, 619
BspLI GGNNCC 6 cut(s) 271, 312, 385, 496, 584, 620
BspPI GGATC 5 cut(s) 277, 305, 318, 577, 590
BspT107I GGYRCC 2 cut(s) 269, 494
BspTNI GGTCTC 2 cut(s) 452, 563
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 3 cut(s) 41, 299, 497
BsrI ACTGG 2 cut(s) 123, 339
BssAI RCCGGY 3 cut(s) 41, 299, 497
BssECI CCNNGG 7 cut(s) 91, 273, 444, 453, 480, 610, 632
BssMI GATC 4 cut(s) 226, 282, 310, 582
BssNI GRCGYC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 51
BssT1I CCWWGG 5 cut(s) 91, 444, 453, 480, 610
Bst2BI CACGAG 1 cut(s) 51
Bst2UI CCWGG 4 cut(s) 275, 287, 347, 634
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 2 cut(s) 248, 551
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 135, 301, 595
BstDSI CCRYGG 1 cut(s) 453
BstHHI GCGC 2 cut(s) 98, 299
BstKTI GATC 4 cut(s) 229, 285, 313, 585
BstMAI GTCTC 2 cut(s) 452, 563
BstMBI GATC 4 cut(s) 226, 282, 310, 582
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 6 cut(s) 197, 206, 392, 437, 446, 603
BstNI CCWGG 4 cut(s) 275, 287, 347, 634
BstSCI CCNGG 5 cut(s) 273, 285, 345, 523, 632
BstSLI GKGCMC 2 cut(s) 274, 497
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 3 cut(s) 282, 310, 582
BstYI RGATCY 3 cut(s) 282, 310, 582
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 7 cut(s) 63, 149, 332, 449, 528, 606, 619
BtgI CCRYGG 1 cut(s) 453
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 346, 504
Cac8I GCNNGC 3 cut(s) 135, 301, 595
CaiI CAGNNNCTG 2 cut(s) 16, 286
CfoI GCGC 2 cut(s) 98, 299
Cfr10I RCCGGY 3 cut(s) 41, 299, 497
Cfr13I GGNCC 3 cut(s) 277, 527, 618
CseI GACGC 1 cut(s) 292
Csp6I GTAC 1 cut(s) 319
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 2 cut(s) 454, 463
CviQI GTAC 1 cut(s) 319
DpnI GATC 4 cut(s) 228, 284, 312, 584
DpnII GATC 4 cut(s) 226, 282, 310, 582
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 3 cut(s) 61, 330, 604
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 248, 551
EarI CTCTTC 2 cut(s) 248, 551
Ecl136II GAGCTC 2 cut(s) 180, 487
EclXI CGGCCG 1 cut(s) 61
Eco130I CCWWGG 5 cut(s) 91, 444, 453, 480, 610
Eco24I GRGCYC 2 cut(s) 182, 489
Eco31I GGTCTC 2 cut(s) 452, 563
Eco47I GGWCC 1 cut(s) 277
Eco52I CGGCCG 1 cut(s) 61
Eco53kI GAGCTC 2 cut(s) 180, 487
EcoICRI GAGCTC 2 cut(s) 180, 487
EcoRII CCWGG 4 cut(s) 273, 285, 345, 632
EcoT14I CCWWGG 5 cut(s) 91, 444, 453, 480, 610
EcoT38I GRGCYC 2 cut(s) 182, 489
ErhI CCWWGG 5 cut(s) 91, 444, 453, 480, 610
FaeI CATG 2 cut(s) 457, 466
FaiI YATR 6 cut(s) 220, 362, 435, 455, 464, 516
FatI CATG 2 cut(s) 453, 462
Fnu4HI GCNGC 3 cut(s) 192, 408, 450
FriOI GRGCYC 2 cut(s) 182, 489
Fsp4HI GCNGC 3 cut(s) 192, 408, 450
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 97, 298
GluI GCNGC 3 cut(s) 192, 408, 450
HaeIII GGCC 7 cut(s) 63, 149, 332, 449, 528, 606, 619
HapII CCGG 4 cut(s) 42, 300, 498, 525
HgaI GACGC 1 cut(s) 292
HhaI GCGC 2 cut(s) 98, 299
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 457, 466
Hin6I GCGC 2 cut(s) 96, 297
HinP1I GCGC 2 cut(s) 96, 297
HincII GTYRAC 2 cut(s) 292, 373
HindII GTYRAC 2 cut(s) 292, 373
HinfI GANTC 2 cut(s) 13, 417
HpaII CCGG 4 cut(s) 42, 300, 498, 525
HphI GGTGA 2 cut(s) 31, 418
Hpy166II GTNNAC 4 cut(s) 79, 292, 373, 426
Hpy188I TCNGA 3 cut(s) 12, 18, 231
Hpy188III TCNNGA 2 cut(s) 224, 280
Hpy8I GTNNAC 4 cut(s) 79, 292, 373, 426
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 206, 392, 437, 446, 603
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 457, 466
HspAI GCGC 2 cut(s) 96, 297
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 4 cut(s) 226, 282, 310, 582
LmnI GCTCC 3 cut(s) 383, 484, 526
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 3 cut(s) 66, 510, 580
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 37
MalI GATC 4 cut(s) 228, 284, 312, 584
MboI GATC 4 cut(s) 226, 282, 310, 582
MboII GAAGA 3 cut(s) 235, 538, 541
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 3 cut(s) 282, 310, 582
MhlI GDGCHC 4 cut(s) 182, 274, 489, 497
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 6 cut(s) 33, 69, 143, 263, 471, 643
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 1 cut(s) 411
MnlI CCTC 8 cut(s) 92, 160, 167, 251, 451, 554, 559, 609
Mox20I TGGCCA 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 606
Msp20I TGGCCA 1 cut(s) 606
MspI CCGG 4 cut(s) 42, 300, 498, 525
MspR9I CCNGG 5 cut(s) 275, 287, 347, 525, 634
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 4 cut(s) 275, 287, 347, 634
MwoI GCNNNNNNNGC 6 cut(s) 197, 206, 392, 437, 446, 603
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 525
NcoI CCATGG 1 cut(s) 453
NdeII GATC 4 cut(s) 226, 282, 310, 582
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 2 cut(s) 457, 466
NlaIV GGNNCC 6 cut(s) 271, 312, 385, 496, 584, 620
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 345
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 3 cut(s) 193, 409, 451
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
Psp124BI GAGCTC 2 cut(s) 182, 489
Psp6I CCWGG 4 cut(s) 273, 285, 345, 632
PspGI CCWGG 4 cut(s) 273, 285, 345, 632
PspN4I GGNNCC 6 cut(s) 271, 312, 385, 496, 584, 620
PspPI GGNCC 3 cut(s) 277, 527, 618
PstNI CAGNNNCTG 2 cut(s) 16, 286
PsuI RGATCY 3 cut(s) 282, 310, 582
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 2 cut(s) 182, 489
SatI GCNGC 3 cut(s) 192, 408, 450
Sau3AI GATC 4 cut(s) 226, 282, 310, 582
Sau96I GGNCC 3 cut(s) 277, 527, 618
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 5 cut(s) 275, 287, 347, 525, 634
SduI GDGCHC 4 cut(s) 182, 274, 489, 497
SetI ASST 8 cut(s) 84, 159, 182, 279, 489, 546, 612, 638
SfaNI GCATC 3 cut(s) 66, 510, 580
SgrAI CRCCGGYG 2 cut(s) 41, 299
SinI GGWCC 1 cut(s) 277
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 6 cut(s) 33, 69, 143, 263, 471, 643
SsiI CCGC 3 cut(s) 408, 429, 450
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 2 cut(s) 182, 489
StyD4I CCNGG 5 cut(s) 273, 285, 345, 523, 632
StyI CCWWGG 5 cut(s) 91, 444, 453, 480, 610
TaaI ACNGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 246, 540, 641
TasI AATT 6 cut(s) 33, 69, 143, 263, 471, 643
TauI GCSGC 2 cut(s) 410, 452
TfiI GAWTC 1 cut(s) 13
TscAI CASTG 2 cut(s) 346, 511
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 207, 248
TspRI CASTG 2 cut(s) 346, 511
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 91
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.