MD03G1214200.v1.1

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008711
Physical Location & Seq
Reverse (-)
29388108 .. 29388937
830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1214200.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGCTGAACACTGCTCTTCCTCTTCCCTTTCCCCTTCCCTGCCCCTCAACGGCCGCGTAGCCATCGTAACCGGCGGTTCGCGCGGCATCGGCCGTGCCATTGCAGCTCACCTCCACTCCCTCGGCGCAAAGGTCGTTGTCAATTACGCTTCATCAAACTCAGCGCAAGCAGATCAATTAGTCTCGGAGCTCAACCAGACAACCCCAAAATCGCAAGCGATTGCGGTCCGAGCAGACGTCTCGGACCCGGATCAGGTGAAGCAGCTCTTCGACAAAGCCGAGCAAGAATTCGGGACCCAACTCGACATCGTCGTGAACAGCGCGGGGGTTTTGGATCCCAAATATCCCAGCGTGGCCAATACGGCGGTGAAGGACTGGGACAACACATTCAATGTCAACACCAAAGGGGCGTTTTTGGTTTCTAGGGAAGCAGCGAAGAGAGTCAAGCGCGGCGGCGGCGGGAGAATTATTATGATAACGTCGTCGGTCGTCGGGGGGCTTATGCCTGGGTACGCAGCCTATGCGGCCTCCAAGGCGGCGGTGGAGACGATGGTAAAGATTTTGGCCAAGGAGCTCAAGGGCACTGGAATAACTGCGAATTGCGTTGCGCCGGGGCCGGTGGCGACGGAGTTGTTCTTTGCCGGGAAGAGTGAGGAGATGGTTAAGAGGCTTGAGGATGCTTGCCCTCTGGGTCGATTGGGCGAGCCCAAGGATGTGTCTGGGATTGTGGGGTTTTTAGCGGCGGATGCAGGCGAGTGGGTCAATGGTCAGGTCATTCGGATCAACGGTGGATTTTTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

268

Amino Acids

27.82

Weight (kDa)

8.38

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 19 - 212 5.8e-51 short chain dehydrogenase
SDR PF23441 21 - 267 2.1e-13 SDR-like rossmann domain
KR PF08659 22 - 197 5.7e-18 KR domain
Epimerase PF01370 22 - 216 5.7e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 28 - 265 4e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AccII CGCG 5 cut(s) 57, 82, 84, 323, 450
AclWI GGATC 4 cut(s) 258, 329, 342, 788
AcoI YGGCCR 4 cut(s) 52, 91, 354, 564
AcsI RAATTY 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 50, 253
AgsI TTSAA 1 cut(s) 391
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 4 cut(s) 107, 190, 265, 574
AluI AGCT 4 cut(s) 107, 190, 265, 574
Alw21I GWGCWC 2 cut(s) 192, 576
Alw26I GTCTC 3 cut(s) 187, 244, 539
AlwI GGATC 4 cut(s) 258, 329, 342, 788
AoxI GGCC 6 cut(s) 52, 91, 354, 525, 564, 614
ApeKI GCWGC 4 cut(s) 104, 262, 431, 515
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 6 cut(s) 84, 128, 166, 323, 450, 610
AspS9I GGNCC 4 cut(s) 226, 244, 294, 614
AsuC2I CCSGG 3 cut(s) 248, 612, 643
AsuHPI GGTGA 3 cut(s) 101, 268, 379
AvaII GGWCC 3 cut(s) 226, 244, 294
BaeGI GKGCMC 1 cut(s) 584
BalI TGGCCA 2 cut(s) 356, 566
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 3 cut(s) 192, 576, 708
Bbv12I GWGCWC 2 cut(s) 192, 576
BbvI GCAGC 4 cut(s) 116, 274, 443, 527
BccI CCATC 3 cut(s) 71, 544, 652
BceAI ACGGC 3 cut(s) 67, 78, 378
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 1 cut(s) 507
BcnI CCSGG 3 cut(s) 248, 612, 643
BcoDI GTCTC 3 cut(s) 187, 244, 539
BfaI CTAG 1 cut(s) 423
BglI GCCNNNNNGGC 3 cut(s) 362, 524, 533
Bme1390I CCNGG 4 cut(s) 248, 507, 612, 643
Bme18I GGWCC 3 cut(s) 226, 244, 294
BmgT120I GGNCC 4 cut(s) 226, 244, 294, 614
BmiI GGNNCC 5 cut(s) 246, 295, 296, 336, 615
BmrFI CCNGG 4 cut(s) 248, 507, 612, 643
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 3 cut(s) 96, 667, 736
BmuI ACTGGG 1 cut(s) 385
BpuEI CTTGAG 2 cut(s) 560, 692
BpuMI CCSGG 3 cut(s) 248, 612, 643
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 6 cut(s) 121, 506, 531, 567, 611, 708
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 253
Bse118I RCCGGY 2 cut(s) 71, 616
Bse1I ACTGG 2 cut(s) 380, 589
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 6 cut(s) 121, 506, 531, 567, 611, 708
BseGI GGATG 3 cut(s) 682, 718, 751
BseLI CCNNNNNNNGG 2 cut(s) 50, 253
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 380, 589
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 1 cut(s) 584
BseX3I CGGCCG 2 cut(s) 52, 91
BseXI GCAGC 4 cut(s) 116, 274, 443, 527
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 5 cut(s) 57, 82, 84, 323, 450
Bsh1285I CGRYCG 3 cut(s) 55, 94, 489
BshFI GGCC 6 cut(s) 54, 93, 356, 527, 566, 616
BsiEI CGRYCG 3 cut(s) 55, 94, 489
BsiHKAI GWGCWC 2 cut(s) 192, 576
BsiSI CCGG 5 cut(s) 72, 248, 611, 617, 642
BslFI GGGAC 2 cut(s) 307, 392
BslI CCNNNNNNNGG 2 cut(s) 50, 253
BsmAI GTCTC 3 cut(s) 187, 244, 539
BsmBI CGTCTC 2 cut(s) 244, 539
BsmFI GGGAC 2 cut(s) 307, 392
BsnI GGCC 6 cut(s) 54, 93, 356, 527, 566, 616
Bsp1286I GDGCHC 4 cut(s) 192, 576, 584, 708
Bsp143I GATC 4 cut(s) 172, 250, 334, 780
BspANI GGCC 6 cut(s) 54, 93, 356, 527, 566, 616
BspCNI CTCAG 1 cut(s) 173
BspFNI CGCG 5 cut(s) 57, 82, 84, 323, 450
BspLI GGNNCC 5 cut(s) 246, 295, 296, 336, 615
BspPI GGATC 4 cut(s) 258, 329, 342, 788
BspQI GCTCTTC 2 cut(s) 22, 272
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 71, 616
BsrI ACTGG 2 cut(s) 380, 589
BssAI RCCGGY 2 cut(s) 71, 616
BssECI CCNNGG 6 cut(s) 121, 506, 531, 567, 611, 708
BssMI GATC 4 cut(s) 172, 250, 334, 780
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 3 cut(s) 531, 567, 708
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 1 cut(s) 788
Bst6I CTCTTC 5 cut(s) 22, 28, 272, 431, 641
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 5 cut(s) 168, 216, 682, 704, 751
BstDEI CTNAG 1 cut(s) 160
BstF5I GGATG 3 cut(s) 682, 718, 751
BstFNI CGCG 5 cut(s) 57, 82, 84, 323, 450
BstHHI GCGC 6 cut(s) 84, 128, 166, 323, 450, 610
BstKTI GATC 4 cut(s) 175, 253, 337, 783
BstMAI GTCTC 3 cut(s) 187, 244, 539
BstMBI GATC 4 cut(s) 172, 250, 334, 780
BstMCI CGRYCG 3 cut(s) 55, 94, 489
BstNI CCWGG 1 cut(s) 507
BstSCI CCNGG 4 cut(s) 246, 505, 610, 641
BstSLI GKGCMC 1 cut(s) 584
BstUI CGCG 5 cut(s) 57, 82, 84, 323, 450
BstV1I GCAGC 4 cut(s) 116, 274, 443, 527
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BstZI CGGCCG 2 cut(s) 52, 91
BsuRI GGCC 6 cut(s) 54, 93, 356, 527, 566, 616
BtsCI GGATG 3 cut(s) 682, 718, 751
BtsI GCAGTG 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 10, 582
Cac8I GCNNGC 5 cut(s) 168, 216, 682, 704, 751
CfoI GCGC 6 cut(s) 84, 128, 166, 323, 450, 610
Cfr10I RCCGGY 2 cut(s) 71, 616
Cfr13I GGNCC 4 cut(s) 226, 244, 294, 614
CpoI CGGWCCG 1 cut(s) 226
Csp6I GTAC 1 cut(s) 511
CspI CGGWCCG 1 cut(s) 226
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 1 cut(s) 160
DpnI GATC 4 cut(s) 174, 252, 336, 782
DpnII GATC 4 cut(s) 172, 250, 334, 780
EaeI YGGCCR 4 cut(s) 52, 91, 354, 564
EagI CGGCCG 2 cut(s) 52, 91
Eam1104I CTCTTC 5 cut(s) 22, 28, 272, 431, 641
EarI CTCTTC 5 cut(s) 22, 28, 272, 431, 641
EciI GGCGGA 1 cut(s) 758
Ecl136II GAGCTC 2 cut(s) 190, 574
EclXI CGGCCG 2 cut(s) 52, 91
Eco130I CCWWGG 3 cut(s) 531, 567, 708
Eco24I GRGCYC 3 cut(s) 192, 576, 708
Eco47I GGWCC 3 cut(s) 226, 244, 294
Eco52I CGGCCG 2 cut(s) 52, 91
Eco53kI GAGCTC 2 cut(s) 190, 574
EcoICRI GAGCTC 2 cut(s) 190, 574
EcoO109I RGGNCCY 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 3 cut(s) 531, 567, 708
EcoT38I GRGCYC 3 cut(s) 192, 576, 708
ErhI CCWWGG 3 cut(s) 531, 567, 708
Esp3I CGTCTC 2 cut(s) 244, 539
FaiI YATR 3 cut(s) 473, 503, 522
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 2 cut(s) 307, 392
FauI CCCGC 2 cut(s) 316, 452
FokI GGATG 3 cut(s) 689, 725, 758
FriOI GRGCYC 3 cut(s) 192, 576, 708
FspBI CTAG 1 cut(s) 423
GlaI GCGC 6 cut(s) 83, 127, 165, 322, 449, 609
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 6 cut(s) 54, 93, 356, 527, 566, 616
HapII CCGG 5 cut(s) 72, 248, 611, 617, 642
HhaI GCGC 6 cut(s) 84, 128, 166, 323, 450, 610
Hin1I GRCGYC 1 cut(s) 237
Hin6I GCGC 6 cut(s) 82, 126, 164, 321, 448, 608
HinP1I GCGC 6 cut(s) 82, 126, 164, 321, 448, 608
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 1 cut(s) 441
HpaII CCGG 5 cut(s) 72, 248, 611, 617, 642
HphI GGTGA 3 cut(s) 101, 268, 379
Hpy166II GTNNAC 2 cut(s) 316, 397
Hpy188I TCNGA 4 cut(s) 187, 230, 244, 780
Hpy188III TCNNGA 2 cut(s) 292, 313
Hpy8I GTNNAC 2 cut(s) 316, 397
Hpy99I CGWCG 5 cut(s) 314, 484, 487, 494, 628
HpyAV CCTTC 2 cut(s) 45, 364
HpyCH4III ACNGT 1 cut(s) 788
HpyCH4IV ACGT 2 cut(s) 237, 479
HpyCH4V TGCA 2 cut(s) 104, 749
HpyF3I CTNAG 1 cut(s) 160
HpySE526I ACGT 2 cut(s) 237, 479
Hsp92I GRCGYC 1 cut(s) 237
HspAI GCGC 6 cut(s) 82, 126, 164, 321, 448, 608
KflI GGGWCCC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 172, 250, 334, 780
LguI GCTCTTC 2 cut(s) 22, 272
LmnI GCTCC 2 cut(s) 187, 571
Lsp1109I GCAGC 4 cut(s) 116, 274, 443, 527
LweI GCATC 3 cut(s) 96, 667, 736
MaeI CTAG 1 cut(s) 423
MaeII ACGT 2 cut(s) 237, 479
MaeIII GTNAC 1 cut(s) 67
MalI GATC 4 cut(s) 174, 252, 336, 782
MboI GATC 4 cut(s) 172, 250, 334, 780
MboII GAAGA 5 cut(s) 9, 15, 259, 448, 658
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 4 cut(s) 192, 576, 584, 708
MlsI TGGCCA 2 cut(s) 356, 566
MluCI AATT 5 cut(s) 142, 176, 287, 465, 598
MluNI TGGCCA 2 cut(s) 356, 566
MlyI GAGTC 1 cut(s) 450
MnlI CCTC 9 cut(s) 31, 56, 122, 131, 538, 646, 660, 667, 696
Mox20I TGGCCA 2 cut(s) 356, 566
MscI TGGCCA 2 cut(s) 356, 566
MseI TTAA 1 cut(s) 663
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 2 cut(s) 356, 566
MspI CCGG 5 cut(s) 72, 248, 611, 617, 642
MspR9I CCNGG 4 cut(s) 248, 507, 612, 643
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 5 cut(s) 57, 82, 84, 323, 450
NciI CCSGG 3 cut(s) 248, 612, 643
NdeII GATC 4 cut(s) 172, 250, 334, 780
NlaIV GGNNCC 5 cut(s) 246, 295, 296, 336, 615
NmeAIII GCCGAG 2 cut(s) 102, 304
PciSI GCTCTTC 2 cut(s) 22, 272
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 309
PflFI GACNNNGTC 1 cut(s) 308
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 192, 576
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 5 cut(s) 246, 295, 296, 336, 615
PspPI GGNCC 4 cut(s) 226, 244, 294, 614
PspPPI RGGWCCY 1 cut(s) 294
PsuI RGATCY 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 311
Rsr2I CGGWCCG 1 cut(s) 226
RsrII CGGWCCG 1 cut(s) 226
SacI GAGCTC 2 cut(s) 192, 576
SapI GCTCTTC 2 cut(s) 22, 272
SaqAI TTAA 1 cut(s) 663
Sau3AI GATC 4 cut(s) 172, 250, 334, 780
Sau96I GGNCC 4 cut(s) 226, 244, 294, 614
SchI GAGTC 1 cut(s) 450
ScrFI CCNGG 4 cut(s) 248, 507, 612, 643
SduI GDGCHC 4 cut(s) 192, 576, 584, 708
SfaNI GCATC 3 cut(s) 96, 667, 736
SinI GGWCC 3 cut(s) 226, 244, 294
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 575, 671
SmoI CTYRAG 2 cut(s) 575, 671
Sse9I AATT 5 cut(s) 142, 176, 287, 465, 598
SspMI CTAG 1 cut(s) 423
SstI GAGCTC 2 cut(s) 192, 576
StyD4I CCNGG 4 cut(s) 246, 505, 610, 641
StyI CCWWGG 3 cut(s) 531, 567, 708
TaaI ACNGT 1 cut(s) 788
TaiI ACGT 2 cut(s) 240, 482
TaqI TCGA 3 cut(s) 270, 303, 694
TaqII GACCGA 1 cut(s) 475
TasI AATT 5 cut(s) 142, 176, 287, 465, 598
TauI GCSGC 8 cut(s) 57, 87, 453, 456, 459, 527, 539, 743
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 2 cut(s) 17, 589
TseI GCWGC 4 cut(s) 104, 262, 431, 515
TspDTI ATGAA 2 cut(s) 141, 787
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 2 cut(s) 17, 589
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 3 cut(s) 226, 244, 294
XapI RAATTY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XspI CTAG 1 cut(s) 423
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.