RLG00000022463

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008711
Physical Location & Seq
Forward (+)
798612 .. 799391
780 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022463

Sequence Viewer

Length: 780 bp
ATGGCTTCTCAGAATCTGAATGGGCGGGTGGCAATTGTCACCGGCGGTTCTCGTGGCATCGGCCGTGAAATTGTTGTCCACCTCCACTCCCTAGGCGCAAGACTCGTCATCAACTACGCTTCCAGTTCCAAGCAGGCAGACGAATTGGCCTCCCACCTCAACAGCAACAACAACAAGAGCTCACAGTTGGCGGCCATTGCAGTAAAAGCAGATGTTTCAGATCCAGATCAGGTGAAGCTCCTCTTCGACAAAGCCGAGCAAGAATTGGGTGCCCAGGTCCAGATCCTGGTCAACTGCGCCGGCGTCTTGGATCCCAAGTACCCGTCTCTGGCCGACACCAGTGTCCAGGATTGGGATAACACATTCAATATCAACACCAAAGGGGCCTTCTTGGCTTGCAGAGAGGCCCCCAACAGACTCCCACGCGGCGGCGGCGGAAGAATTATCATGATTACCACCTCCGTTGTTGGGGGGCTGATGCCCGGATACGCCGCCTATGCTGCCTCCAAGGCCGCCGTGGAGACCATGACCAAAATTGTCGCCAAGGAGCTCAAGGGCACCGGCATCACTGCCAACTGTGTTGCTCCCGGTCCTGTTGCCACCGACCTCTTCTTCGCCGGTAAAAGTGAGGAGACCATCAACAGGATCAAGGATGCTTGCCCTCTTGGCCGCCTTGGTCAGCCCCACGATGTGTCCCAGGTTGTCGGATTTTTAGCCGGCGATGCAGGGGAGTGGATCAACGGTCAGGTCATTAGGGTTAATGGTGGATCTCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

260

Amino Acids

27.06

Weight (kDa)

7.68

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 9 - 204 9.3e-47 short chain dehydrogenase
SDR PF23441 9 - 258 2.3e-09 SDR-like rossmann domain
KR PF08659 12 - 181 1.4e-12 KR domain
adh_short_C2 PF13561 18 - 256 1.8e-53 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 269, 557
AccB7I CCANNNNNTGG 1 cut(s) 286
AccII CGCG 1 cut(s) 426
AclWI GGATC 7 cut(s) 215, 277, 305, 318, 653, 743, 775
AcoI YGGCCR 4 cut(s) 61, 192, 330, 667
AcyI GRCGYC 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 6 cut(s) 286, 328, 352, 428, 468, 642
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 4 cut(s) 273, 285, 345, 696
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 3 cut(s) 180, 238, 550
AluI AGCT 3 cut(s) 180, 238, 550
Alw21I GWGCWC 2 cut(s) 182, 552
Alw26I GTCTC 3 cut(s) 330, 515, 626
AlwI GGATC 7 cut(s) 215, 277, 305, 318, 653, 743, 775
AlwNI CAGNNNCTG 2 cut(s) 16, 286
AoxI GGCC 8 cut(s) 61, 147, 192, 330, 384, 405, 510, 667
ApeKI GCWGC 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspA2I CCTAGG 1 cut(s) 91
AspLEI GCGC 2 cut(s) 98, 299
AspS9I GGNCC 4 cut(s) 277, 384, 406, 590
AsuC2I CCSGG 2 cut(s) 483, 588
AsuHPI GGTGA 2 cut(s) 31, 244
AvaII GGWCC 2 cut(s) 277, 590
AvrII CCTAGG 1 cut(s) 91
BaeGI GKGCMC 2 cut(s) 274, 560
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 269, 557
BanII GRGCYC 2 cut(s) 182, 552
BauI CACGAG 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 182, 552
BbvI GCAGC 1 cut(s) 487
BccI CCATC 1 cut(s) 644
BceAI ACGGC 2 cut(s) 48, 500
BciT130I CCWGG 4 cut(s) 275, 287, 347, 698
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 2 cut(s) 483, 588
BcoDI GTCTC 3 cut(s) 330, 515, 626
BfaI CTAG 1 cut(s) 92
BfuI GTATCC 1 cut(s) 479
BglI GCCNNNNNGGC 3 cut(s) 392, 509, 666
BisI GCNGC 8 cut(s) 192, 427, 430, 433, 492, 501, 513, 670
BlnI CCTAGG 1 cut(s) 91
BlsI GCNGC 8 cut(s) 193, 428, 431, 434, 493, 502, 514, 671
Bme1390I CCNGG 6 cut(s) 275, 287, 347, 483, 588, 698
Bme18I GGWCC 2 cut(s) 277, 590
BmgT120I GGNCC 4 cut(s) 277, 384, 406, 590
BmiI GGNNCC 5 cut(s) 271, 312, 385, 408, 559
BmrFI CCNGG 6 cut(s) 275, 287, 347, 483, 588, 698
BmsI GCATC 5 cut(s) 66, 468, 573, 643, 712
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 2 cut(s) 483, 588
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 2 cut(s) 515, 626
BsaJI CCNNGG 7 cut(s) 91, 273, 507, 516, 543, 673, 696
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 6 cut(s) 286, 328, 352, 428, 468, 642
Bse118I RCCGGY 5 cut(s) 41, 299, 560, 617, 716
Bse1I ACTGG 2 cut(s) 123, 339
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 4 cut(s) 275, 287, 347, 698
BseDI CCNNGG 7 cut(s) 91, 273, 507, 516, 543, 673, 696
BseGI GGATG 1 cut(s) 658
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 6 cut(s) 286, 328, 352, 428, 468, 642
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 123, 339
BseRI GAGGAG 2 cut(s) 230, 644
BseSI GKGCMC 2 cut(s) 274, 560
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 8 cut(s) 63, 149, 194, 332, 386, 407, 512, 669
BshNI GGYRCC 2 cut(s) 269, 557
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 2 cut(s) 182, 552
BsiSI CCGG 7 cut(s) 42, 300, 483, 561, 588, 618, 717
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 6 cut(s) 286, 328, 352, 428, 468, 642
BsmAI GTCTC 3 cut(s) 330, 515, 626
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 679
BsnI GGCC 8 cut(s) 63, 149, 194, 332, 386, 407, 512, 669
Bso31I GGTCTC 2 cut(s) 515, 626
Bsp1286I GDGCHC 4 cut(s) 182, 274, 552, 560
Bsp143I GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
BspANI GGCC 8 cut(s) 63, 149, 194, 332, 386, 407, 512, 669
BspCNI CTCAG 1 cut(s) 22
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 5 cut(s) 271, 312, 385, 408, 559
BspPI GGATC 7 cut(s) 215, 277, 305, 318, 653, 743, 775
BspT107I GGYRCC 2 cut(s) 269, 557
BspTNI GGTCTC 2 cut(s) 515, 626
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 5 cut(s) 41, 299, 560, 617, 716
BsrI ACTGG 2 cut(s) 123, 339
BssAI RCCGGY 5 cut(s) 41, 299, 560, 617, 716
BssECI CCNNGG 7 cut(s) 91, 273, 507, 516, 543, 673, 696
BssMI GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
BssNI GRCGYC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 51
BssT1I CCWWGG 4 cut(s) 91, 507, 543, 673
Bst2BI CACGAG 1 cut(s) 51
Bst2UI CCWGG 4 cut(s) 275, 287, 347, 698
Bst4CI ACNGT 3 cut(s) 186, 578, 743
Bst6I CTCTTC 2 cut(s) 248, 614
BstACI GRCGYC 1 cut(s) 303
BstC8I GCNNGC 5 cut(s) 135, 301, 397, 658, 718
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 1 cut(s) 658
BstFNI CGCG 1 cut(s) 426
BstHHI GCGC 2 cut(s) 98, 299
BstKTI GATC 7 cut(s) 223, 229, 285, 313, 648, 738, 770
BstMAI GTCTC 3 cut(s) 330, 515, 626
BstMBI GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 9 cut(s) 197, 206, 392, 432, 497, 500, 509, 666, 722
BstNI CCWGG 4 cut(s) 275, 287, 347, 698
BstSCI CCNGG 6 cut(s) 273, 285, 345, 481, 586, 696
BstSLI GKGCMC 2 cut(s) 274, 560
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 487
BstX2I RGATCY 4 cut(s) 220, 282, 310, 767
BstYI RGATCY 4 cut(s) 220, 282, 310, 767
BstZI CGGCCG 1 cut(s) 61
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 8 cut(s) 63, 149, 194, 332, 386, 407, 512, 669
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 1 cut(s) 658
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 346, 567
Cac8I GCNNGC 5 cut(s) 135, 301, 397, 658, 718
CaiI CAGNNNCTG 2 cut(s) 16, 286
CciI TCATGA 1 cut(s) 447
CfoI GCGC 2 cut(s) 98, 299
Cfr10I RCCGGY 5 cut(s) 41, 299, 560, 617, 716
Cfr13I GGNCC 4 cut(s) 277, 384, 406, 590
CseI GACGC 1 cut(s) 292
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 2 cut(s) 448, 526
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 9
DpnI GATC 7 cut(s) 222, 228, 284, 312, 647, 737, 769
DpnII GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
DraIII CACNNNGTG 1 cut(s) 691
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 4 cut(s) 61, 192, 330, 667
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 248, 614
EarI CTCTTC 2 cut(s) 248, 614
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 2 cut(s) 180, 550
EclXI CGGCCG 1 cut(s) 61
Eco130I CCWWGG 4 cut(s) 91, 507, 543, 673
Eco24I GRGCYC 2 cut(s) 182, 552
Eco31I GGTCTC 2 cut(s) 515, 626
Eco47I GGWCC 2 cut(s) 277, 590
Eco52I CGGCCG 1 cut(s) 61
Eco53kI GAGCTC 2 cut(s) 180, 550
EcoICRI GAGCTC 2 cut(s) 180, 550
EcoO109I RGGNCCY 2 cut(s) 384, 406
EcoRII CCWGG 4 cut(s) 273, 285, 345, 696
EcoT14I CCWWGG 4 cut(s) 91, 507, 543, 673
EcoT38I GRGCYC 2 cut(s) 182, 552
ErhI CCWWGG 4 cut(s) 91, 507, 543, 673
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 2 cut(s) 451, 529
FaiI YATR 3 cut(s) 449, 498, 527
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 1 cut(s) 679
FatI CATG 2 cut(s) 447, 525
FauI CCCGC 1 cut(s) 18
Fnu4HI GCNGC 8 cut(s) 192, 427, 430, 433, 492, 501, 513, 670
FokI GGATG 1 cut(s) 665
FriOI GRGCYC 2 cut(s) 182, 552
Fsp4HI GCNGC 8 cut(s) 192, 427, 430, 433, 492, 501, 513, 670
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 97, 298
GluI GCNGC 8 cut(s) 192, 427, 430, 433, 492, 501, 513, 670
HaeIII GGCC 8 cut(s) 63, 149, 194, 332, 386, 407, 512, 669
HapII CCGG 7 cut(s) 42, 300, 483, 561, 588, 618, 717
HgaI GACGC 1 cut(s) 292
HhaI GCGC 2 cut(s) 98, 299
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 451, 529
Hin6I GCGC 2 cut(s) 96, 297
HinP1I GCGC 2 cut(s) 96, 297
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 3 cut(s) 13, 102, 417
HpaII CCGG 7 cut(s) 42, 300, 483, 561, 588, 618, 717
HphI GGTGA 2 cut(s) 31, 244
Hpy166II GTNNAC 2 cut(s) 79, 292
Hpy188I TCNGA 5 cut(s) 12, 18, 220, 707, 779
Hpy188III TCNNGA 3 cut(s) 224, 280, 448
Hpy8I GTNNAC 2 cut(s) 79, 292
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 3 cut(s) 186, 578, 743
HpyCH4V TGCA 3 cut(s) 200, 399, 725
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 206, 392, 432, 497, 500, 509, 666, 722
HpyF3I CTNAG 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 451, 529
HspAI GCGC 2 cut(s) 96, 297
KroI GCCGGC 2 cut(s) 299, 716
KroNI GCCGGC 2 cut(s) 301, 718
Kzo9I GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
LmnI GCTCC 3 cut(s) 243, 547, 589
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 5 cut(s) 66, 468, 573, 643, 712
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 37
MalI GATC 7 cut(s) 222, 228, 284, 312, 647, 737, 769
MboI GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
MboII GAAGA 4 cut(s) 235, 450, 601, 604
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 4 cut(s) 220, 282, 310, 767
MhlI GDGCHC 4 cut(s) 182, 274, 552, 560
MluCI AATT 6 cut(s) 33, 69, 143, 263, 441, 534
MlyI GAGTC 2 cut(s) 96, 411
MmeI TCCRAC 1 cut(s) 685
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 2 cut(s) 299, 716
MseI TTAA 1 cut(s) 759
MspI CCGG 7 cut(s) 42, 300, 483, 561, 588, 618, 717
MspR9I CCNGG 6 cut(s) 275, 287, 347, 483, 588, 698
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 4 cut(s) 275, 287, 347, 698
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 9 cut(s) 197, 206, 392, 432, 497, 500, 509, 666, 722
NaeI GCCGGC 2 cut(s) 301, 718
NciI CCSGG 2 cut(s) 483, 588
NdeII GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
NgoMIV GCCGGC 2 cut(s) 299, 716
NlaIII CATG 2 cut(s) 451, 529
NlaIV GGNNCC 5 cut(s) 271, 312, 385, 408, 559
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 37
PagI TCATGA 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PdiI GCCGGC 2 cut(s) 301, 718
PfeI GAWTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 8 cut(s) 193, 428, 431, 434, 493, 502, 514, 671
PleI GAGTC 2 cut(s) 96, 411
PpsI GAGTC 2 cut(s) 96, 411
Psp124BI GAGCTC 2 cut(s) 182, 552
Psp6I CCWGG 4 cut(s) 273, 285, 345, 696
PspGI CCWGG 4 cut(s) 273, 285, 345, 696
PspN4I GGNNCC 5 cut(s) 271, 312, 385, 408, 559
PspPI GGNCC 4 cut(s) 277, 384, 406, 590
PstNI CAGNNNCTG 2 cut(s) 16, 286
PsuI RGATCY 4 cut(s) 220, 282, 310, 767
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 2 cut(s) 182, 552
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 8 cut(s) 192, 427, 430, 433, 492, 501, 513, 670
Sau3AI GATC 7 cut(s) 220, 226, 282, 310, 645, 735, 767
Sau96I GGNCC 4 cut(s) 277, 384, 406, 590
SchI GAGTC 2 cut(s) 96, 411
ScrFI CCNGG 6 cut(s) 275, 287, 347, 483, 588, 698
SduI GDGCHC 4 cut(s) 182, 274, 552, 560
SfaNI GCATC 5 cut(s) 66, 468, 573, 643, 712
SgrAI CRCCGGYG 2 cut(s) 41, 299
SinI GGWCC 2 cut(s) 277, 590
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 6 cut(s) 33, 69, 143, 263, 441, 534
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 2 cut(s) 182, 552
StyD4I CCNGG 6 cut(s) 273, 285, 345, 481, 586, 696
StyI CCWWGG 4 cut(s) 91, 507, 543, 673
TaaI ACNGT 3 cut(s) 186, 578, 743
TaqI TCGA 1 cut(s) 246
TasI AATT 6 cut(s) 33, 69, 143, 263, 441, 534
TauI GCSGC 7 cut(s) 194, 429, 432, 435, 494, 515, 672
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 2 cut(s) 346, 574
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 2 cut(s) 346, 574
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 277, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmaJI CCTAGG 1 cut(s) 91
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.