MD14G1002100.v1.1

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008711
Physical Location & Seq
Reverse (-)
351884 .. 352711
828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1002100.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGCTGCACAAACTCCTACCAGAGAAGCTGAACACTACTCTTCTTCTTCCCCTTCCCCTTCCCTGCCCCTCAACGGCCGCGTAGCCATCGTAACCGGCGGTTCGCGCGGTATCGGCCGTGCCATTGCAGCTCACCTCCACTCCCTCGGCGCAAAGGTCGTCGTCAATTACGCTTCATCAAACTCAGCGCAAGCAGATCAATTAGTCTCCGAGCTCAACCAGACAACCCCAAAATCGCAAGCGATTGCCGTCCGAGCAGACATCTCGGACCCGGACCAGGTGAAGCAGCTCTTCGACAAAGCCGAGCAAGAATTCGGGACCCAACTCCACATCGTCGTGAACAGCGCGGGCGCTTTGGATCCCAAATATCCTAGCGTGGCCAATACGGCGGTGGAGGACTGGGACAACACATTCAATGTCAACACCAAGGGGGCGTTTTTGGTTTCTAGGGAAGCGGCGAATAGGGTTACGCGCGGCGGCGGTGGGAGAATTATTATGATTACATCGTCCGTCGTCGGGCGGCTTCCGCCTGGGTACGCAGCCTACGCGGCCTCCAAGACGGCGGTGGAGACGATGGTAAAGATTTTGGCCAAGGAGCTCAAGGGCACCGGAATAACTGCCAATTGCGTTGCGCCGGGGCCGGTGGCGACGGAGATGTTCTTTGCCGGGAAGAGTGAGGAGATGGTTAAGGGGATTGAGGATGCTTGCCCTCTGGGTCGATTGGGCGAGCCCAAGGATGTGTGTGGGATTGTGGGGTTTTTAGCGGCGGATGCTGGGGAGTGGGTCAACGGCCAGGTCATTCGGATCAACGGTGGATTTGTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

276

Amino Acids

28.67

Weight (kDa)

6.44

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 27 - 220 2.6e-49 short chain dehydrogenase
SDR PF23441 29 - 273 5.1e-11 SDR-like rossmann domain
KR PF08659 30 - 205 4.9e-18 KR domain
Epimerase PF01370 30 - 207 2.6e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 36 - 273 2.1e-57 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AccII CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
AclWI GGATC 3 cut(s) 353, 366, 812
AcoI YGGCCR 5 cut(s) 76, 115, 378, 588, 790
AcsI RAATTY 1 cut(s) 311
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 3 cut(s) 74, 277, 516
AgsI TTSAA 1 cut(s) 415
AjnI CCWGG 3 cut(s) 276, 529, 792
AluBI AGCT 5 cut(s) 29, 131, 214, 289, 598
AluI AGCT 5 cut(s) 29, 131, 214, 289, 598
Alw21I GWGCWC 2 cut(s) 216, 600
Alw26I GTCTC 2 cut(s) 211, 563
AlwI GGATC 3 cut(s) 353, 366, 812
AoxI GGCC 7 cut(s) 76, 115, 378, 549, 588, 638, 790
ApeKI GCWGC 4 cut(s) 5, 128, 286, 539
ApoI RAATTY 1 cut(s) 311
AspLEI GCGC 7 cut(s) 108, 152, 190, 347, 353, 474, 634
AspS9I GGNCC 4 cut(s) 268, 274, 318, 638
AsuC2I CCSGG 3 cut(s) 272, 636, 667
AsuHPI GGTGA 2 cut(s) 125, 292
AvaII GGWCC 3 cut(s) 268, 274, 318
BaeGI GKGCMC 1 cut(s) 608
BalI TGGCCA 2 cut(s) 380, 590
BamHI GGATCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 3 cut(s) 216, 600, 732
Bbv12I GWGCWC 2 cut(s) 216, 600
BbvI GCAGC 3 cut(s) 140, 298, 551
BccI CCATC 3 cut(s) 95, 568, 676
BceAI ACGGC 6 cut(s) 91, 102, 233, 402, 576, 805
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 3 cut(s) 278, 531, 794
BcnI CCSGG 3 cut(s) 272, 636, 667
BcoDI GTCTC 2 cut(s) 211, 563
BfaI CTAG 2 cut(s) 372, 447
BfoI RGCGCY 1 cut(s) 354
BglI GCCNNNNNGGC 2 cut(s) 386, 548
Bme1390I CCNGG 6 cut(s) 272, 278, 531, 636, 667, 794
Bme18I GGWCC 3 cut(s) 268, 274, 318
BmgT120I GGNCC 4 cut(s) 268, 274, 318, 638
BmiI GGNNCC 6 cut(s) 270, 319, 320, 360, 607, 639
BmrFI CCNGG 6 cut(s) 272, 278, 531, 636, 667, 794
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 2 cut(s) 691, 760
BmuI ACTGGG 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 3 cut(s) 272, 636, 667
BsaJI CCNNGG 6 cut(s) 145, 426, 530, 591, 635, 732
BsaWI WCCGGW 1 cut(s) 608
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 536, 566
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 277, 516
Bse118I RCCGGY 2 cut(s) 95, 640
Bse1I ACTGG 1 cut(s) 404
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 3 cut(s) 278, 531, 794
BseDI CCNNGG 6 cut(s) 145, 426, 530, 591, 635, 732
BseGI GGATG 3 cut(s) 706, 742, 775
BseLI CCNNNNNNNGG 3 cut(s) 74, 277, 516
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 608
BseX3I CGGCCG 2 cut(s) 76, 115
BseXI GCAGC 3 cut(s) 140, 298, 551
BseYI CCCAGC 1 cut(s) 773
Bsh1236I CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
Bsh1285I CGRYCG 2 cut(s) 79, 118
BshFI GGCC 7 cut(s) 78, 117, 380, 551, 590, 640, 792
BshNI GGYRCC 1 cut(s) 605
BsiEI CGRYCG 2 cut(s) 79, 118
BsiHKAI GWGCWC 2 cut(s) 216, 600
BsiSI CCGG 6 cut(s) 96, 272, 609, 635, 641, 666
BslFI GGGAC 2 cut(s) 331, 416
BslI CCNNNNNNNGG 3 cut(s) 74, 277, 516
BsmAI GTCTC 2 cut(s) 211, 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 331, 416
BsnI GGCC 7 cut(s) 78, 117, 380, 551, 590, 640, 792
Bsp1286I GDGCHC 4 cut(s) 216, 600, 608, 732
Bsp143I GATC 3 cut(s) 196, 358, 804
BspANI GGCC 7 cut(s) 78, 117, 380, 551, 590, 640, 792
BspCNI CTCAG 1 cut(s) 197
BspFNI CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
BspLI GGNNCC 6 cut(s) 270, 319, 320, 360, 607, 639
BspPI GGATC 3 cut(s) 353, 366, 812
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 95, 640
BsrI ACTGG 1 cut(s) 404
BssAI RCCGGY 2 cut(s) 95, 640
BssECI CCNNGG 6 cut(s) 145, 426, 530, 591, 635, 732
BssMI GATC 3 cut(s) 196, 358, 804
BssT1I CCWWGG 3 cut(s) 426, 591, 732
Bst2UI CCWGG 3 cut(s) 278, 531, 794
Bst4CI ACNGT 1 cut(s) 812
Bst6I CTCTTC 3 cut(s) 46, 296, 665
BstC8I GCNNGC 5 cut(s) 192, 240, 349, 706, 728
BstDEI CTNAG 1 cut(s) 184
BstF5I GGATG 3 cut(s) 706, 742, 775
BstFNI CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 7 cut(s) 108, 152, 190, 347, 353, 474, 634
BstKTI GATC 3 cut(s) 199, 361, 807
BstMAI GTCTC 2 cut(s) 211, 563
BstMBI GATC 3 cut(s) 196, 358, 804
BstMCI CGRYCG 2 cut(s) 79, 118
BstMWI GCNNNNNNNGC 9 cut(s) 105, 114, 128, 254, 386, 526, 545, 548, 770
BstNI CCWGG 3 cut(s) 278, 531, 794
BstSCI CCNGG 6 cut(s) 270, 276, 529, 634, 665, 792
BstSLI GKGCMC 1 cut(s) 608
BstUI CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
BstV1I GCAGC 3 cut(s) 140, 298, 551
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BstZI CGGCCG 2 cut(s) 76, 115
BsuRI GGCC 7 cut(s) 78, 117, 380, 551, 590, 640, 792
BtsCI GGATG 3 cut(s) 706, 742, 775
Cac8I GCNNGC 5 cut(s) 192, 240, 349, 706, 728
CfoI GCGC 7 cut(s) 108, 152, 190, 347, 353, 474, 634
Cfr10I RCCGGY 2 cut(s) 95, 640
Cfr13I GGNCC 4 cut(s) 268, 274, 318, 638
CsiI ACCWGGT 1 cut(s) 276
Csp6I GTAC 1 cut(s) 535
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 1 cut(s) 184
DpnI GATC 3 cut(s) 198, 360, 806
DpnII GATC 3 cut(s) 196, 358, 804
EaeI YGGCCR 5 cut(s) 76, 115, 378, 588, 790
EagI CGGCCG 2 cut(s) 76, 115
Eam1104I CTCTTC 3 cut(s) 46, 296, 665
EarI CTCTTC 3 cut(s) 46, 296, 665
EciI GGCGGA 2 cut(s) 516, 782
Ecl136II GAGCTC 2 cut(s) 214, 598
EclXI CGGCCG 2 cut(s) 76, 115
Eco130I CCWWGG 3 cut(s) 426, 591, 732
Eco24I GRGCYC 3 cut(s) 216, 600, 732
Eco47I GGWCC 3 cut(s) 268, 274, 318
Eco52I CGGCCG 2 cut(s) 76, 115
Eco53kI GAGCTC 2 cut(s) 214, 598
EcoICRI GAGCTC 2 cut(s) 214, 598
EcoO109I RGGNCCY 1 cut(s) 318
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 3 cut(s) 276, 529, 792
EcoT14I CCWWGG 3 cut(s) 426, 591, 732
EcoT38I GRGCYC 3 cut(s) 216, 600, 732
ErhI CCWWGG 3 cut(s) 426, 591, 732
Esp3I CGTCTC 1 cut(s) 563
FaiI YATR 1 cut(s) 497
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FaqI GGGAC 2 cut(s) 331, 416
FauI CCCGC 1 cut(s) 340
FokI GGATG 3 cut(s) 713, 749, 782
FriOI GRGCYC 3 cut(s) 216, 600, 732
FspBI CTAG 2 cut(s) 372, 447
GlaI GCGC 7 cut(s) 107, 151, 189, 346, 352, 473, 633
GsaI CCCAGC 1 cut(s) 777
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 7 cut(s) 78, 117, 380, 551, 590, 640, 792
HapII CCGG 6 cut(s) 96, 272, 609, 635, 641, 666
HhaI GCGC 7 cut(s) 108, 152, 190, 347, 353, 474, 634
Hin6I GCGC 7 cut(s) 106, 150, 188, 345, 351, 472, 632
HinP1I GCGC 7 cut(s) 106, 150, 188, 345, 351, 472, 632
HincII GTYRAC 2 cut(s) 421, 787
HindII GTYRAC 2 cut(s) 421, 787
HpaII CCGG 6 cut(s) 96, 272, 609, 635, 641, 666
HphI GGTGA 2 cut(s) 125, 292
Hpy166II GTNNAC 3 cut(s) 340, 421, 787
Hpy188I TCNGA 4 cut(s) 211, 254, 268, 804
Hpy188III TCNNGA 2 cut(s) 316, 337
Hpy8I GTNNAC 3 cut(s) 340, 421, 787
Hpy99I CGWCG 5 cut(s) 164, 338, 515, 518, 652
HpyAV CCTTC 2 cut(s) 63, 69
HpyCH4III ACNGT 1 cut(s) 812
HpyCH4V TGCA 2 cut(s) 8, 128
HpyF10VI GCNNNNNNNGC 9 cut(s) 105, 114, 128, 254, 386, 526, 545, 548, 770
HpyF3I CTNAG 1 cut(s) 184
HspAI GCGC 7 cut(s) 106, 150, 188, 345, 351, 472, 632
KflI GGGWCCC 1 cut(s) 318
Kzo9I GATC 3 cut(s) 196, 358, 804
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 3 cut(s) 140, 298, 551
LweI GCATC 2 cut(s) 691, 760
MabI ACCWGGT 1 cut(s) 276
MaeI CTAG 2 cut(s) 372, 447
MaeIII GTNAC 2 cut(s) 91, 466
MalI GATC 3 cut(s) 198, 360, 806
MboI GATC 3 cut(s) 196, 358, 804
MboII GAAGA 5 cut(s) 33, 36, 39, 283, 682
MfeI CAATTG 1 cut(s) 622
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 4 cut(s) 216, 600, 608, 732
MlsI TGGCCA 2 cut(s) 380, 590
MluCI AATT 5 cut(s) 166, 200, 311, 489, 622
MluNI TGGCCA 2 cut(s) 380, 590
MnlI CCTC 8 cut(s) 80, 146, 155, 388, 562, 670, 691, 720
Mox20I TGGCCA 2 cut(s) 380, 590
MscI TGGCCA 2 cut(s) 380, 590
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 2 cut(s) 380, 590
MspI CCGG 6 cut(s) 96, 272, 609, 635, 641, 666
MspR9I CCNGG 6 cut(s) 272, 278, 531, 636, 667, 794
MunI CAATTG 1 cut(s) 622
MvaI CCWGG 3 cut(s) 278, 531, 794
MvnI CGCG 7 cut(s) 81, 106, 108, 347, 472, 474, 548
MwoI GCNNNNNNNGC 9 cut(s) 105, 114, 128, 254, 386, 526, 545, 548, 770
NciI CCSGG 3 cut(s) 272, 636, 667
NdeII GATC 3 cut(s) 196, 358, 804
NlaIV GGNNCC 6 cut(s) 270, 319, 320, 360, 607, 639
NmeAIII GCCGAG 2 cut(s) 126, 328
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 318
Psp124BI GAGCTC 2 cut(s) 216, 600
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 3 cut(s) 276, 529, 792
PspFI CCCAGC 1 cut(s) 773
PspGI CCWGG 3 cut(s) 276, 529, 792
PspN4I GGNNCC 6 cut(s) 270, 319, 320, 360, 607, 639
PspPI GGNCC 4 cut(s) 268, 274, 318, 638
PspPPI RGGWCCY 1 cut(s) 318
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 216, 600
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 687
Sau3AI GATC 3 cut(s) 196, 358, 804
Sau96I GGNCC 4 cut(s) 268, 274, 318, 638
ScrFI CCNGG 6 cut(s) 272, 278, 531, 636, 667, 794
SduI GDGCHC 4 cut(s) 216, 600, 608, 732
SetI ASST 9 cut(s) 31, 133, 138, 159, 216, 282, 291, 600, 798
SexAI ACCWGGT 1 cut(s) 276
SfaNI GCATC 2 cut(s) 691, 760
SinI GGWCC 3 cut(s) 268, 274, 318
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 5 cut(s) 166, 200, 311, 489, 622
SspMI CTAG 2 cut(s) 372, 447
SstI GAGCTC 2 cut(s) 216, 600
StyD4I CCNGG 6 cut(s) 270, 276, 529, 634, 665, 792
StyI CCWWGG 3 cut(s) 426, 591, 732
TaaI ACNGT 1 cut(s) 812
TaqI TCGA 2 cut(s) 294, 718
TasI AATT 5 cut(s) 166, 200, 311, 489, 622
TauI GCSGC 7 cut(s) 81, 458, 477, 480, 523, 551, 767
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TseI GCWGC 4 cut(s) 5, 128, 286, 539
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 2 cut(s) 499, 665
VpaK11BI GGWCC 3 cut(s) 268, 274, 318
XapI RAATTY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 2 cut(s) 388, 562
XspI CTAG 2 cut(s) 372, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.