pycom03g16290

Short-chain type dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008711
Physical Location & Seq
Reverse (-)
17452330 .. 17453139
810 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16290.1

Sequence Viewer

Length: 810 bp
ATGGCTGCGCAAACTACTACCAGCGAAGCTGAGCACTGCTCTTCCTCTTCCCTGCCCCTCAACGGCCGCGTAGCCATCGTAACCGGCGGTTCGCGCGGCATCGGCCGTGCCATTGCAGCTCACCTCCACTCCCTTGGCGCAAAGGTCGTTGTCAATTACGCTTCATCAAACTCGGCGCAAGCAGATCAATTAGTCTCAGAGCTCAACCAGACAACCCCAAAATCGCAAGCGATTGCGGTCCGAGCAGACGTCTCGGACCCGGATCAGGTGAAGCAGCTCTTCGACAAAGCCGAGCAAGAATTCGGGACCCAACTCGACATCGTCGTGAACAGCGCGGGGGTTTTGGATCCCAAATATCCCAGCGTGGCCAATACGGCGGTGGAGGACTGGGACAACACATTCAATGTCAACACCAAGGGGGCGTTTTTGGTTTCTAGGGAAGCGGCGAATAGAGTCAAGCGCGGCGGCGGCGGGAGAATTATTATGATAACGTCGTCGGTCGTCGGGGGGCTTATGCCTGGGTACGCAGCCTATGCGGCCTCCAAGGCGGCGGTGGAGACGATGGTAAAGATTTTGGCCAAGGAGCTCAAGGGCACCGGAATAACTGCGAATTGCGTTGCGCCGGGGCCGGTGGCGACGGAGTTGTTCTTTGCCGGGAAGAGTGAGGAGACGGTTAAGAGGCTTGAGGATGCTTGCCCTCTGGGTCGGTTGGGCGAGCCCAAGGATGTGTCTGGGATTGTGGGGTTTTTAGCGGCGGATGCAGGCGAGTGGGTCAATGGTCAGGTCATTCGGATCAACGGTGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

270

Amino Acids

27.92

Weight (kDa)

5.97

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 23 - 216 7.2e-51 short chain dehydrogenase
SDR PF23441 25 - 268 1.4e-12 SDR-like rossmann domain
KR PF08659 26 - 201 2.2e-18 KR domain
Epimerase PF01370 26 - 197 2.6e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 32 - 269 1.1e-59 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
Acc16I TGCGCA 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 593
AccII CGCG 5 cut(s) 69, 94, 96, 335, 462
AclWI GGATC 4 cut(s) 270, 341, 354, 800
AcoI YGGCCR 4 cut(s) 64, 103, 366, 576
AcsI RAATTY 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 2 cut(s) 62, 265
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 5 cut(s) 29, 119, 202, 277, 586
AluI AGCT 5 cut(s) 29, 119, 202, 277, 586
Alw21I GWGCWC 3 cut(s) 36, 204, 588
Alw26I GTCTC 4 cut(s) 199, 256, 551, 662
AlwI GGATC 4 cut(s) 270, 341, 354, 800
AoxI GGCC 6 cut(s) 64, 103, 366, 537, 576, 626
ApeKI GCWGC 4 cut(s) 5, 116, 274, 527
ApoI RAATTY 1 cut(s) 299
AspLEI GCGC 7 cut(s) 10, 96, 140, 178, 335, 462, 622
AspS9I GGNCC 4 cut(s) 238, 256, 306, 626
AsuC2I CCSGG 3 cut(s) 260, 624, 655
AsuHPI GGTGA 2 cut(s) 113, 280
AvaII GGWCC 3 cut(s) 238, 256, 306
BaeGI GKGCMC 1 cut(s) 596
BalI TGGCCA 2 cut(s) 368, 578
BamHI GGATCC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 593
BanII GRGCYC 3 cut(s) 204, 588, 720
Bbv12I GWGCWC 3 cut(s) 36, 204, 588
BbvI GCAGC 3 cut(s) 128, 286, 539
BccI CCATC 2 cut(s) 83, 556
BceAI ACGGC 3 cut(s) 79, 90, 390
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 519
BcnI CCSGG 3 cut(s) 260, 624, 655
BcoDI GTCTC 4 cut(s) 199, 256, 551, 662
BfaI CTAG 1 cut(s) 435
BglI GCCNNNNNGGC 3 cut(s) 374, 536, 545
BlpI GCTNAGC 1 cut(s) 30
Bme1390I CCNGG 4 cut(s) 260, 519, 624, 655
Bme18I GGWCC 3 cut(s) 238, 256, 306
BmgT120I GGNCC 4 cut(s) 238, 256, 306, 626
BmiI GGNNCC 6 cut(s) 258, 307, 308, 348, 595, 627
BmrFI CCNGG 4 cut(s) 260, 519, 624, 655
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 3 cut(s) 108, 679, 748
BmuI ACTGGG 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 572, 704
BpuMI CCSGG 3 cut(s) 260, 624, 655
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 7 cut(s) 133, 414, 518, 543, 579, 623, 720
BsaWI WCCGGW 1 cut(s) 596
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 265
Bse118I RCCGGY 2 cut(s) 83, 628
Bse1I ACTGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 7 cut(s) 133, 414, 518, 543, 579, 623, 720
BseGI GGATG 3 cut(s) 694, 730, 763
BseLI CCNNNNNNNGG 2 cut(s) 62, 265
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 21, 210
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 596
BseX3I CGGCCG 2 cut(s) 64, 103
BseXI GCAGC 3 cut(s) 128, 286, 539
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 5 cut(s) 69, 94, 96, 335, 462
Bsh1285I CGRYCG 3 cut(s) 67, 106, 501
BshFI GGCC 6 cut(s) 66, 105, 368, 539, 578, 628
BshNI GGYRCC 1 cut(s) 593
BsiEI CGRYCG 3 cut(s) 67, 106, 501
BsiHKAI GWGCWC 3 cut(s) 36, 204, 588
BsiSI CCGG 6 cut(s) 84, 260, 597, 623, 629, 654
BslFI GGGAC 2 cut(s) 319, 404
BslI CCNNNNNNNGG 2 cut(s) 62, 265
BsmAI GTCTC 4 cut(s) 199, 256, 551, 662
BsmBI CGTCTC 3 cut(s) 256, 551, 662
BsmFI GGGAC 2 cut(s) 319, 404
BsnI GGCC 6 cut(s) 66, 105, 368, 539, 578, 628
Bsp1286I GDGCHC 5 cut(s) 36, 204, 588, 596, 720
Bsp143I GATC 4 cut(s) 184, 262, 346, 792
Bsp1720I GCTNAGC 1 cut(s) 30
BspANI GGCC 6 cut(s) 66, 105, 368, 539, 578, 628
BspCNI CTCAG 2 cut(s) 22, 209
BspFNI CGCG 5 cut(s) 69, 94, 96, 335, 462
BspLI GGNNCC 6 cut(s) 258, 307, 308, 348, 595, 627
BspPI GGATC 4 cut(s) 270, 341, 354, 800
BspQI GCTCTTC 2 cut(s) 46, 284
BspT107I GGYRCC 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 2 cut(s) 83, 628
BsrI ACTGG 1 cut(s) 392
BssAI RCCGGY 2 cut(s) 83, 628
BssECI CCNNGG 7 cut(s) 133, 414, 518, 543, 579, 623, 720
BssMI GATC 4 cut(s) 184, 262, 346, 792
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 5 cut(s) 133, 414, 543, 579, 720
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 2 cut(s) 673, 800
Bst6I CTCTTC 4 cut(s) 46, 52, 284, 653
BstACI GRCGYC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 5 cut(s) 180, 228, 694, 716, 763
BstDEI CTNAG 2 cut(s) 30, 196
BstF5I GGATG 3 cut(s) 694, 730, 763
BstFNI CGCG 5 cut(s) 69, 94, 96, 335, 462
BstHHI GCGC 7 cut(s) 10, 96, 140, 178, 335, 462, 622
BstKTI GATC 4 cut(s) 187, 265, 349, 795
BstMAI GTCTC 4 cut(s) 199, 256, 551, 662
BstMBI GATC 4 cut(s) 184, 262, 346, 792
BstMCI CGRYCG 3 cut(s) 67, 106, 501
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 4 cut(s) 258, 517, 622, 653
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 5 cut(s) 69, 94, 96, 335, 462
BstV1I GCAGC 3 cut(s) 128, 286, 539
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 134
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 2 cut(s) 64, 103
BsuRI GGCC 6 cut(s) 66, 105, 368, 539, 578, 628
BtsCI GGATG 3 cut(s) 694, 730, 763
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 5 cut(s) 180, 228, 694, 716, 763
CfoI GCGC 7 cut(s) 10, 96, 140, 178, 335, 462, 622
Cfr10I RCCGGY 2 cut(s) 83, 628
Cfr13I GGNCC 4 cut(s) 238, 256, 306, 626
CpoI CGGWCCG 1 cut(s) 238
Csp6I GTAC 1 cut(s) 523
CspI CGGWCCG 1 cut(s) 238
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 2 cut(s) 30, 196
DpnI GATC 4 cut(s) 186, 264, 348, 794
DpnII GATC 4 cut(s) 184, 262, 346, 792
EaeI YGGCCR 4 cut(s) 64, 103, 366, 576
EagI CGGCCG 2 cut(s) 64, 103
Eam1104I CTCTTC 4 cut(s) 46, 52, 284, 653
EarI CTCTTC 4 cut(s) 46, 52, 284, 653
EciI GGCGGA 1 cut(s) 770
Ecl136II GAGCTC 2 cut(s) 202, 586
EclXI CGGCCG 2 cut(s) 64, 103
Eco130I CCWWGG 5 cut(s) 133, 414, 543, 579, 720
Eco24I GRGCYC 3 cut(s) 204, 588, 720
Eco47I GGWCC 3 cut(s) 238, 256, 306
Eco52I CGGCCG 2 cut(s) 64, 103
Eco53kI GAGCTC 2 cut(s) 202, 586
EcoICRI GAGCTC 2 cut(s) 202, 586
EcoO109I RGGNCCY 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 517
EcoT14I CCWWGG 5 cut(s) 133, 414, 543, 579, 720
EcoT38I GRGCYC 3 cut(s) 204, 588, 720
ErhI CCWWGG 5 cut(s) 133, 414, 543, 579, 720
Esp3I CGTCTC 3 cut(s) 256, 551, 662
FaiI YATR 3 cut(s) 485, 515, 534
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 319, 404
FauI CCCGC 2 cut(s) 328, 464
FokI GGATG 3 cut(s) 701, 737, 770
FriOI GRGCYC 3 cut(s) 204, 588, 720
FspBI CTAG 1 cut(s) 435
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 7 cut(s) 9, 95, 139, 177, 334, 461, 621
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 6 cut(s) 66, 105, 368, 539, 578, 628
HapII CCGG 6 cut(s) 84, 260, 597, 623, 629, 654
HhaI GCGC 7 cut(s) 10, 96, 140, 178, 335, 462, 622
Hin1I GRCGYC 1 cut(s) 249
Hin6I GCGC 7 cut(s) 8, 94, 138, 176, 333, 460, 620
HinP1I GCGC 7 cut(s) 8, 94, 138, 176, 333, 460, 620
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 1 cut(s) 453
HpaII CCGG 6 cut(s) 84, 260, 597, 623, 629, 654
HphI GGTGA 2 cut(s) 113, 280
Hpy166II GTNNAC 2 cut(s) 328, 409
Hpy188I TCNGA 4 cut(s) 199, 242, 256, 792
Hpy188III TCNNGA 2 cut(s) 304, 325
Hpy8I GTNNAC 2 cut(s) 328, 409
Hpy99I CGWCG 5 cut(s) 326, 496, 499, 506, 640
HpyCH4III ACNGT 2 cut(s) 673, 800
HpyCH4IV ACGT 2 cut(s) 249, 491
HpyCH4V TGCA 2 cut(s) 116, 761
HpyF3I CTNAG 2 cut(s) 30, 196
HpySE526I ACGT 2 cut(s) 249, 491
Hsp92I GRCGYC 1 cut(s) 249
HspAI GCGC 7 cut(s) 8, 94, 138, 176, 333, 460, 620
KflI GGGWCCC 1 cut(s) 306
Kzo9I GATC 4 cut(s) 184, 262, 346, 792
LguI GCTCTTC 2 cut(s) 46, 284
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 3 cut(s) 128, 286, 539
LweI GCATC 3 cut(s) 108, 679, 748
MaeI CTAG 1 cut(s) 435
MaeII ACGT 2 cut(s) 249, 491
MaeIII GTNAC 1 cut(s) 79
MalI GATC 4 cut(s) 186, 264, 348, 794
MboI GATC 4 cut(s) 184, 262, 346, 792
MboII GAAGA 4 cut(s) 33, 39, 271, 670
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 5 cut(s) 36, 204, 588, 596, 720
MlsI TGGCCA 2 cut(s) 368, 578
MluCI AATT 5 cut(s) 154, 188, 299, 477, 610
MluNI TGGCCA 2 cut(s) 368, 578
MlyI GAGTC 1 cut(s) 462
MnlI CCTC 9 cut(s) 55, 68, 134, 376, 550, 658, 672, 679, 708
Mox20I TGGCCA 2 cut(s) 368, 578
MscI TGGCCA 2 cut(s) 368, 578
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 323
Msp20I TGGCCA 2 cut(s) 368, 578
MspI CCGG 6 cut(s) 84, 260, 597, 623, 629, 654
MspR9I CCNGG 4 cut(s) 260, 519, 624, 655
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 5 cut(s) 69, 94, 96, 335, 462
NciI CCSGG 3 cut(s) 260, 624, 655
NdeII GATC 4 cut(s) 184, 262, 346, 792
NlaIV GGNNCC 6 cut(s) 258, 307, 308, 348, 595, 627
NmeAIII GCCGAG 2 cut(s) 152, 316
NsbI TGCGCA 1 cut(s) 9
PciSI GCTCTTC 2 cut(s) 46, 284
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 321
PflFI GACNNNGTC 1 cut(s) 320
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PpuMI RGGWCCY 1 cut(s) 306
Psp124BI GAGCTC 2 cut(s) 204, 588
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 6 cut(s) 258, 307, 308, 348, 595, 627
PspPI GGNCC 4 cut(s) 238, 256, 306, 626
PspPPI RGGWCCY 1 cut(s) 306
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 1 cut(s) 323
Rsr2I CGGWCCG 1 cut(s) 238
RsrII CGGWCCG 1 cut(s) 238
SacI GAGCTC 2 cut(s) 204, 588
SapI GCTCTTC 2 cut(s) 46, 284
SaqAI TTAA 1 cut(s) 675
Sau3AI GATC 4 cut(s) 184, 262, 346, 792
Sau96I GGNCC 4 cut(s) 238, 256, 306, 626
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 4 cut(s) 260, 519, 624, 655
SduI GDGCHC 5 cut(s) 36, 204, 588, 596, 720
SfaNI GCATC 3 cut(s) 108, 679, 748
SinI GGWCC 3 cut(s) 238, 256, 306
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 587, 683
SmoI CTYRAG 2 cut(s) 587, 683
Sse9I AATT 5 cut(s) 154, 188, 299, 477, 610
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 2 cut(s) 204, 588
StyD4I CCNGG 4 cut(s) 258, 517, 622, 653
StyI CCWWGG 5 cut(s) 133, 414, 543, 579, 720
TaaI ACNGT 2 cut(s) 673, 800
TaiI ACGT 2 cut(s) 252, 494
TaqI TCGA 2 cut(s) 282, 315
TaqII GACCGA 1 cut(s) 487
TasI AATT 5 cut(s) 154, 188, 299, 477, 610
TauI GCSGC 9 cut(s) 69, 99, 446, 465, 468, 471, 539, 551, 755
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TscAI CASTG 1 cut(s) 41
TseI GCWGC 4 cut(s) 5, 116, 274, 527
TspDTI ATGAA 1 cut(s) 153
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 1 cut(s) 41
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 3 cut(s) 238, 256, 306
XapI RAATTY 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 550
XspI CTAG 1 cut(s) 435
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.