FvH4_4g27170

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004080
Physical Location & Seq
Forward (+)
28291655 .. 28292963
1309 bp
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UTR
Exon/CDS
Intron
FvH4_4g27170.t1

Sequence Viewer

Length: 456 bp
ATGGAGCAGCAGGGAGTCGAGAAGATGAAGCAGAAGGTGATGTTGGCAATTGATGAGAGCGAGCTCAGCCACTACGCCCTCGAATGGACCCTCGAAAACCTTGGCAACACCATTCACAATTCTGAGCTACTCGTCTTCACTGCACAACCCATCACCATTGATTTTGCTCCTGAGTTGGTAGCAGCTCTACAAGAAAACTACAAGAAGGTTGCATCGGGTTTGCTGGACAAAGCTAAAAACATCTGCTCCAATTATGGGATTGTTGCAAGAACAGTAACAGAGATTGGTGATCCTAAAAAGGCAATATGTGAAGCAGTAGAAAAATTCAACATCGAGTTACTAGTATTAGGCAGTCATGGTCGAGGCATTGTGCAAAGGGCTTTCCTGGGAAGCGTCAGCAATTACTGCATTCACAATGCCAGATGTACTGTTCTTGTTGTGAGGAAACCAATGTGA

Protein Analysis

152

Amino Acids

16.73

Weight (kDa)

6.42

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 11 - 148 1.5e-28 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 84, 255
AgsI TTSAA 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 340
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AluBI AGCT 4 cut(s) 64, 127, 185, 233
AluI AGCT 4 cut(s) 64, 127, 185, 233
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 7, 182
ApoI RAATTY 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 49, 145, 299
AvaII GGWCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 19, 194
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 386
BcuI ACTAGT 1 cut(s) 340
BfaI CTAG 1 cut(s) 341
BisI GCNGC 2 cut(s) 8, 183
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 2 cut(s) 9, 184
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 127
Bpu1102I GCTNAGC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 100, 385
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 255
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 100, 385
BseLI CCNNNNNNNGG 2 cut(s) 84, 255
BseMII CTCAG 3 cut(s) 79, 114, 162
BseXI GCAGC 2 cut(s) 19, 194
BsgI GTGCAG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 66
BslI CCNNNNNNNGG 2 cut(s) 84, 255
BsmI GAATGC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 289
Bsp1720I GCTNAGC 1 cut(s) 65
BspCNI CTCAG 3 cut(s) 78, 115, 163
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 100, 385
BssMI GATC 1 cut(s) 289
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 274, 430
BstAPI GCANNNNNTGC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 65, 123, 171
BstKTI GATC 1 cut(s) 292
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 2 cut(s) 66, 405
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 19, 194
BstV2I GAAGAC 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 426
CviAII CATG 1 cut(s) 356
CviJI RGCY 6 cut(s) 64, 69, 127, 185, 233, 380
CviKI_1 RGCY 6 cut(s) 64, 69, 127, 185, 233, 380
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 3 cut(s) 65, 123, 171
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 359
FaiI YATR 3 cut(s) 255, 307, 357
FatI CATG 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 8, 183
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 8, 183
FspBI CTAG 1 cut(s) 341
GluI GCNGC 2 cut(s) 8, 183
HgaI GACGC 1 cut(s) 382
Hin1II CATG 1 cut(s) 359
HinfI GANTC 1 cut(s) 15
HphI GGTGA 3 cut(s) 49, 145, 299
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 19, 170
HpyAV CCTTC 2 cut(s) 28, 199
HpyCH4III ACNGT 2 cut(s) 274, 430
HpyCH4V TGCA 5 cut(s) 143, 212, 266, 373, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 405
HpyF3I CTNAG 3 cut(s) 65, 123, 171
Hsp92II CATG 1 cut(s) 359
Kzo9I GATC 1 cut(s) 289
LmnI GCTCC 3 cut(s) 4, 172, 251
LpnPI CCDG 5 cut(s) 183, 209, 371, 398, 433
Lsp1109I GCAGC 2 cut(s) 19, 194
LweI GCATC 1 cut(s) 221
MaeI CTAG 1 cut(s) 341
MaeIII GTNAC 2 cut(s) 274, 336
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 2 cut(s) 34, 127
MfeI CAATTG 1 cut(s) 48
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 5 cut(s) 48, 118, 250, 323, 400
MlyI GAGTC 1 cut(s) 24
MnlI CCTC 4 cut(s) 89, 101, 356, 435
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 48
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 66, 405
NdeII GATC 1 cut(s) 289
NlaIII CATG 1 cut(s) 359
NlaIV GGNNCC 1 cut(s) 89
PctI GAATGC 1 cut(s) 408
PkrI GCNGC 2 cut(s) 9, 184
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 87
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SacI GAGCTC 1 cut(s) 66
SatI GCNGC 2 cut(s) 8, 183
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 66
SetI ASST 7 cut(s) 39, 66, 102, 129, 187, 210, 235
SfaNI GCATC 1 cut(s) 221
SinI GGWCC 1 cut(s) 87
SpeI ACTAGT 1 cut(s) 340
Sse9I AATT 5 cut(s) 48, 118, 250, 323, 400
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 2 cut(s) 274, 430
TaqI TCGA 5 cut(s) 18, 81, 93, 333, 361
TasI AATT 5 cut(s) 48, 118, 250, 323, 400
TatI WGTACW 1 cut(s) 425
TscAI CASTG 1 cut(s) 145
TseI GCWGC 2 cut(s) 7, 182
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 323
XspI CTAG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.