Rw4G029350

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004080
Physical Location & Seq
Forward (+)
56024514 .. 56025964
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029350.1

Sequence Viewer

Length: 462 bp
ATGAAGAAGAAGGTGATGGTGGCAATTGATGAGAGCGAGCTTAGCCACTACGCGCTAGAATGGACCCTCGAAAATCTTGGCAACACCATTCATAATTCTGAGCTACTCGTCTTCACTGCACAGCCCACCACCATTGATTTTGGTTACACAAACAATGTTCTCGATCCATTTTCTCCTGAGCTGGTAGCAGCTATACAAGAAAACTACAAGAAGGTGGCATTGGTTTTGCTGGATAAAGCTAAAGACATCTGCGCCAACCATGGGATTGTTGCAGAGACAGTAACAGAGGTTGGTGATCCTAAAAAGGCAATATGTGAAGCGGTAGAAAAATTCAACATCGAGTTACTAATACTAGGCAGTCATGGTCGAGGAACTGTACAAAGGGCTTTCCTGGGAAGTGTCAGCAATTACTGCATTCATAATGCCAGATGTACTGTTCTTGTTGTGAGGAAACCAATTTGA

Protein Analysis

153

Amino Acids

16.88

Weight (kDa)

5.94

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 2 - 150 1.5e-30 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 53
AciI CCGC 1 cut(s) 320
AclWI GGATC 2 cut(s) 158, 290
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 2 cut(s) 378, 433
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 1 cut(s) 334
AjnI CCWGG 1 cut(s) 390
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 5 cut(s) 40, 103, 181, 191, 239
AluI AGCT 5 cut(s) 40, 103, 181, 191, 239
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 2 cut(s) 158, 290
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 329
AspLEI GCGC 2 cut(s) 55, 254
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 25, 305
AvaII GGWCC 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 200
BccI CCATC 1 cut(s) 10
BciT130I CCWGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 56, 353
BisI GCNGC 1 cut(s) 189
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 259, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 259, 391
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 90, 168
BseXI GCAGC 1 cut(s) 200
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 414
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 2 cut(s) 163, 295
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 91, 169
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 2 cut(s) 158, 290
BsrGI TGTACA 1 cut(s) 376
BssECI CCNNGG 2 cut(s) 259, 391
BssMI GATC 2 cut(s) 163, 295
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 3 cut(s) 280, 376, 436
BstAPI GCANNNNNTGC 1 cut(s) 411
BstAUI TGTACA 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 3 cut(s) 41, 99, 177
BstDSI CCRYGG 1 cut(s) 259
BstFNI CGCG 1 cut(s) 53
BstHHI GCGC 2 cut(s) 55, 254
BstKTI GATC 2 cut(s) 166, 298
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 2 cut(s) 163, 295
BstMWI GCNNNNNNNGC 2 cut(s) 42, 411
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 390
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 200
BstV2I GAAGAC 1 cut(s) 103
BtgI CCRYGG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 2 cut(s) 55, 254
Cfr13I GGNCC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 377, 432
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 2 cut(s) 260, 362
CviJI RGCY 8 cut(s) 40, 45, 103, 124, 181, 191, 239, 386
CviKI_1 RGCY 8 cut(s) 40, 45, 103, 124, 181, 191, 239, 386
CviQI GTAC 2 cut(s) 377, 432
DdeI CTNAG 3 cut(s) 41, 99, 177
DpnI GATC 2 cut(s) 165, 297
DpnII GATC 2 cut(s) 163, 295
Eco130I CCWWGG 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 263, 365
FaiI YATR 6 cut(s) 93, 194, 261, 313, 363, 420
FatI CATG 2 cut(s) 259, 361
Fnu4HI GCNGC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 2 cut(s) 56, 353
GlaI GCGC 2 cut(s) 54, 253
GluI GCNGC 1 cut(s) 189
HhaI GCGC 2 cut(s) 55, 254
Hin1II CATG 2 cut(s) 263, 365
Hin6I GCGC 2 cut(s) 53, 252
HinP1I GCGC 2 cut(s) 53, 252
HphI GGTGA 2 cut(s) 25, 305
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 2 cut(s) 161, 176
HpyAV CCTTC 2 cut(s) 4, 205
HpyCH4III ACNGT 3 cut(s) 280, 376, 436
HpyCH4V TGCA 3 cut(s) 119, 272, 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 411
HpyF3I CTNAG 3 cut(s) 41, 99, 177
Hsp92II CATG 2 cut(s) 263, 365
HspAI GCGC 2 cut(s) 53, 252
Kzo9I GATC 2 cut(s) 163, 295
LpnPI CCDG 6 cut(s) 167, 189, 215, 377, 404, 439
Lsp1109I GCAGC 1 cut(s) 200
MaeI CTAG 2 cut(s) 56, 353
MaeIII GTNAC 3 cut(s) 143, 280, 342
MalI GATC 2 cut(s) 165, 297
MboI GATC 2 cut(s) 163, 295
MboII GAAGA 3 cut(s) 16, 19, 103
MfeI CAATTG 1 cut(s) 24
MluCI AATT 5 cut(s) 24, 94, 329, 406, 456
MnlI CCTC 4 cut(s) 77, 280, 362, 441
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 42, 411
NcoI CCATGG 1 cut(s) 259
NdeII GATC 2 cut(s) 163, 295
NlaIII CATG 2 cut(s) 263, 365
NlaIV GGNNCC 1 cut(s) 65
PctI GAATGC 1 cut(s) 414
PkrI GCNGC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 63
RsaI GTAC 2 cut(s) 378, 433
RsaNI GTAC 2 cut(s) 377, 432
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 2 cut(s) 163, 295
Sau96I GGNCC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 392
SetI ASST 8 cut(s) 15, 42, 105, 183, 193, 216, 241, 291
SinI GGWCC 1 cut(s) 63
Sse9I AATT 5 cut(s) 24, 94, 329, 406, 456
SsiI CCGC 1 cut(s) 320
SspMI CTAG 2 cut(s) 56, 353
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 3 cut(s) 280, 376, 436
TaqI TCGA 4 cut(s) 69, 162, 339, 367
TasI AATT 5 cut(s) 24, 94, 329, 406, 456
TatI WGTACW 2 cut(s) 376, 431
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 3 cut(s) 17, 80, 407
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 329
XspI CTAG 2 cut(s) 56, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.