FvH4_4g27180

Universal stress protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004080
Physical Location & Seq
Forward (+)
28294617 .. 28296097
1481 bp
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UTR
Exon/CDS
Intron
FvH4_4g27180.t1

Sequence Viewer

Length: 528 bp
ATGGGTGAGGTAGTAGACAATGGAGGAGCAGCTGAGAGTGGTGAGAAGCCAGTTATGGTGGCAGTTGATGAAAGTGAGTGCAGTCACTATGCTCTCATGTGGGTTATTGACAACCTCAAGGAGTCCATAAATACTAATTCGCCGCTGCTCATCTTCATGGCACAGCCTCCTCCGGCCAACAACATCACATTCGCGGCGCCTCTCGGGTCTGCTCGCATGTATTGTCCTCCCACTCCGGAGTTTACCAACAATATGCAGGAAAATCACAGGAAGCTTACAACGGCTCTGTTGGAGAGAGCTAAAGACATCTGTGCTAGTCATGGGGTGACTGCAAAGACAGTTACAGAGATAGGGGATGCTAAAACAGCCATATGTGCTGCAGTTCTAAAACACAATGTCAAGCTGCTTGTTCTTGGCGAGCGTGGACTTGGAAAAATCAAAAGGGCTATCCTTGGGAGTGTTAGCAACTACTGCGTTCTGAATGCAAAGTGCCCTGTTCTTGTTGTGAAGAAACCACAGGTTGTTTGA

Protein Analysis

176

Amino Acids

18.74

Weight (kDa)

8.21

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 16 - 170 1.7e-23 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 2 cut(s) 143, 194
AcoI YGGCCR 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 197
AluBI AGCT 4 cut(s) 32, 274, 299, 403
AluI AGCT 4 cut(s) 32, 274, 299, 403
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 4 cut(s) 29, 145, 377, 403
AspLEI GCGC 1 cut(s) 199
AsuHPI GGTGA 3 cut(s) 17, 53, 337
AvaI CYCGRG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 196
BbvI GCAGC 4 cut(s) 41, 132, 364, 390
BceAI ACGGC 1 cut(s) 297
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 200
BisI GCNGC 6 cut(s) 30, 143, 146, 195, 378, 404
BlsI GCNGC 6 cut(s) 31, 144, 147, 196, 379, 405
BmeT110I CYCGRG 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 198
BmsI GCATC 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 39, 159
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 4 cut(s) 41, 132, 364, 390
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 176
BshNI GGYRCC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 2 cut(s) 173, 236
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 494
Bsp13I TCCGGA 1 cut(s) 235
BspACI CCGC 2 cut(s) 143, 194
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 25
BspEI TCCGGA 1 cut(s) 235
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 451
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 451
Bst4CI ACNGT 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 214, 419
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 361
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 365, 374, 471
BstNSI RCATGY 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 378
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 4 cut(s) 41, 132, 364, 390
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 214, 419
CfoI GCGC 1 cut(s) 199
CviAII CATG 4 cut(s) 97, 157, 217, 320
DdeI CTNAG 1 cut(s) 33
DinI GGCGCC 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 451
Eco88I CYCGRG 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 451
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 4 cut(s) 100, 160, 220, 323
FatI CATG 4 cut(s) 96, 156, 216, 319
FauNDI CATATG 1 cut(s) 371
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 6 cut(s) 30, 143, 146, 195, 378, 404
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 6 cut(s) 30, 143, 146, 195, 378, 404
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 198
GluI GCNGC 6 cut(s) 30, 143, 146, 195, 378, 404
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 173, 236
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 4 cut(s) 100, 160, 220, 323
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 1 cut(s) 122
HpaII CCGG 2 cut(s) 173, 236
HphI GGTGA 3 cut(s) 17, 53, 337
Hpy166II GTNNAC 3 cut(s) 16, 243, 425
Hpy188I TCNGA 1 cut(s) 480
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 3 cut(s) 16, 243, 425
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4V TGCA 5 cut(s) 81, 256, 332, 380, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 374, 471
HpyF3I CTNAG 1 cut(s) 33
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 4 cut(s) 100, 160, 220, 323
HspAI GCGC 1 cut(s) 197
KasI GGCGCC 1 cut(s) 196
Kpn2I TCCGGA 1 cut(s) 235
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 7 cut(s) 63, 186, 242, 249, 253, 503, 507
Lsp1109I GCAGC 4 cut(s) 41, 132, 364, 390
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 3 cut(s) 83, 325, 340
MboII GAAGA 2 cut(s) 145, 520
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 1 cut(s) 136
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 6 cut(s) 17, 125, 177, 180, 210, 237
MroI TCCGGA 1 cut(s) 235
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 32, 145
MspI CCGG 2 cut(s) 173, 236
Mva1269I GAATGC 1 cut(s) 487
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 3 cut(s) 365, 374, 471
NarI GGCGCC 1 cut(s) 197
NdeI CATATG 1 cut(s) 371
NlaIII CATG 4 cut(s) 100, 160, 220, 323
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 2 cut(s) 83, 325
NspI RCATGY 1 cut(s) 220
PctI GAATGC 1 cut(s) 487
PkrI GCNGC 6 cut(s) 31, 144, 147, 196, 379, 405
PleI GAGTC 1 cut(s) 130
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 155
SatI GCNGC 6 cut(s) 30, 143, 146, 195, 378, 404
SchI GAGTC 1 cut(s) 131
SduI GDGCHC 1 cut(s) 494
SetI ASST 7 cut(s) 12, 34, 117, 276, 301, 405, 522
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 136
SsiI CCGC 2 cut(s) 143, 194
SspDI GGCGCC 1 cut(s) 196
SspMI CTAG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 340
TasI AATT 1 cut(s) 136
TauI GCSGC 2 cut(s) 145, 197
TseFI GTSAC 2 cut(s) 83, 325
TseI GCWGC 4 cut(s) 29, 145, 377, 403
Tsp45I GTSAC 2 cut(s) 83, 325
TspDTI ATGAA 2 cut(s) 84, 145
XceI RCATGY 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.