MD16G1041100.v1.1

Universal stress protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004080
Physical Location & Seq
Forward (+)
2909313 .. 2910948
1636 bp
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UTR
Exon/CDS
Intron
MD16G1041100.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGGGGAAGAAGCGATTGCGAGAGGAGAAGAAAGAATGGGAGAAGCGATTGCAAGAGGAGCTGAGAAGAGGGTGATGGTGGCCATTGACGAGAGCGATTGCAGTCATTATGCGCTCATGTGGGTTCTCGACAATCTCAAAGATTCCATAACCAACTCGCCCTTGATCATCTTCACGGCTCAGACTCCCTCCGCCGGCAACGTCATCTTCTCAGCGTCGCTCGGCAATGCTCGCATGTACTATCCTATGTCTTCCAATCAGGAGTTTGTTAACTCCTCCCAGGAAAATCACAGGAAGTTAGCGTTGGGACTGTTGGAGAAAGCTAAAGAGATCTGTGCTAATCATGGGGTAAATGCAGAGACAGTCACAGAAGTAGGAGATCCGAAAGCAGCCATATGTGACGCTGTCCAAAAACACAACATCAATCTGCTAATTCTAGGCGAGCGTGGGCTGGGAAAAATCAAAAGGGCAATTCTTGGTAGTGTCAGCAACTACTGTGTTCAGAACGCCAAGTGCCCTGTCCTCGTCGTGAAGAAATCACAAGCTGAAGGTGAAGTTGCTTGA

Protein Analysis

188

Amino Acids

20.17

Weight (kDa)

6.1

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 23 - 178 1.5e-28 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 81
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 194
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 3 cut(s) 62, 323, 545
AluI AGCT 3 cut(s) 62, 323, 545
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 1 cut(s) 374
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 85, 563
BaeGI GKGCMC 1 cut(s) 518
BalI TGGCCA 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 401
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 281
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 1 cut(s) 437
BglII AGATCT 1 cut(s) 330
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 232
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 3 cut(s) 54, 194, 225
BseRI GAGGAG 3 cut(s) 39, 72, 265
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 1 cut(s) 401
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 3 cut(s) 165, 330, 379
BspACI CCGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 3 cut(s) 55, 193, 224
BspPI GGATC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 3 cut(s) 165, 330, 379
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 3 cut(s) 312, 364, 497
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 3 cut(s) 196, 232, 443
BstDEI CTNAG 3 cut(s) 63, 180, 211
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 3 cut(s) 168, 333, 382
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 3 cut(s) 165, 330, 379
BstMWI GCNNNNNNNGC 2 cut(s) 59, 231
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 518
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 2 cut(s) 330, 379
BstYI RGATCY 2 cut(s) 330, 379
BsuRI GGCC 1 cut(s) 83
Cac8I GCNNGC 3 cut(s) 196, 232, 443
CfoI GCGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 194
CseI GACGC 2 cut(s) 204, 410
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 118, 235, 344
CviJI RGCY 7 cut(s) 62, 83, 179, 323, 392, 451, 545
CviKI_1 RGCY 7 cut(s) 62, 83, 179, 323, 392, 451, 545
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 3 cut(s) 63, 180, 211
DpnI GATC 3 cut(s) 167, 332, 381
DpnII GATC 3 cut(s) 165, 330, 379
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 279
FaeI CATG 3 cut(s) 121, 238, 347
FaiI YATR 8 cut(s) 111, 119, 149, 236, 248, 345, 395, 397
FaqI GGGAC 1 cut(s) 321
FatI CATG 3 cut(s) 117, 234, 343
FauNDI CATATG 1 cut(s) 395
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 1 cut(s) 437
GlaI GCGC 1 cut(s) 114
GluI GCNGC 1 cut(s) 390
GsaI CCCAGC 1 cut(s) 455
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 195
HgaI GACGC 2 cut(s) 204, 410
HhaI GCGC 1 cut(s) 115
Hin1II CATG 3 cut(s) 121, 238, 347
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 143, 184
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 85, 563
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 3 cut(s) 183, 384, 504
Hpy188III TCNNGA 3 cut(s) 128, 260, 529
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 2 cut(s) 220, 530
HpyAV CCTTC 1 cut(s) 542
HpyCH4III ACNGT 3 cut(s) 312, 364, 497
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 53, 102, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 231
HpyF3I CTNAG 3 cut(s) 63, 180, 211
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 3 cut(s) 121, 238, 347
HspAI GCGC 1 cut(s) 113
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 165
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 3 cut(s) 165, 330, 379
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 7 cut(s) 208, 245, 266, 277, 293, 437, 531
Lsp1109I GCAGC 1 cut(s) 401
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 364, 398
MalI GATC 3 cut(s) 167, 332, 381
MboI GATC 3 cut(s) 165, 330, 379
MboII GAAGA 7 cut(s) 20, 41, 79, 163, 199, 243, 544
MflI RGATCY 2 cut(s) 330, 379
MhlI GDGCHC 1 cut(s) 518
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 2 cut(s) 432, 471
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 6 cut(s) 17, 50, 63, 199, 286, 533
Mox20I TGGCCA 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 194
MscI TGGCCA 1 cut(s) 83
MseI TTAA 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 83
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 59, 231
NaeI GCCGGC 1 cut(s) 196
NdeI CATATG 1 cut(s) 395
NdeII GATC 3 cut(s) 165, 330, 379
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 3 cut(s) 121, 238, 347
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 364, 398
NspI RCATGY 1 cut(s) 238
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 143
PflFI GACNNNGTC 1 cut(s) 404
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 279
PsuI RGATCY 2 cut(s) 330, 379
PsyI GACNNNGTC 1 cut(s) 404
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 3 cut(s) 165, 330, 379
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 518
SetI ASST 5 cut(s) 64, 204, 325, 547, 553
Sse9I AATT 2 cut(s) 432, 471
SsiI CCGC 1 cut(s) 192
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 3 cut(s) 312, 364, 497
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 129
TasI AATT 2 cut(s) 432, 471
TatI WGTACW 1 cut(s) 237
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TseFI GTSAC 2 cut(s) 364, 398
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 2 cut(s) 364, 398
Tth111I GACNNNGTC 1 cut(s) 404
XceI RCATGY 1 cut(s) 238
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.