MD13G1040200.v1.1

Universal stress protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004080
Physical Location & Seq
Forward (+)
2735573 .. 2737214
1642 bp
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UTR
Exon/CDS
Intron
MD13G1040200.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGGAGAGGCGATTCCGAGAGGAGAAGAAAGAATGGGAGAAGTGGTTGCAAAGGGAGCCGAGAAGAAGGTGATGGTGGCCATTGACGAGAGCGAGTGCAGTCATTACGCTCTCATGTGGGTTCTCGACAACCTCAAAGACTCCATAACCAACTCGCCCTTGATCATCTTCATGGCTCAGACTCCCTCCAACGGCAACATCACCTTCTCGGCGTCGCTCGGCGCTGCTCGCATGTACTGTCCTATGTCTTCCAGTCAGGAGTTTGTTAACTCTTCCCAAGAAAATCAAAGGAAGTTTGCGTTGGCACTGTTGGAGAAAGCTAAACAGATCTGTGCTAGTCGTGGGGTAAATGCTGAGACAGTCACAGAAGTTGGAGATCCGAAAGAAGCCATATGTGATGCTGTCAAAAAACACGGCATCAAGCTGCTAGTTGTAGGCGAGCGTGGGCTCGGAAAAATCAAAAGGGCAATTCTTGGTAGTGTCAGCGACTACTGTGTTCAGCACGCCAATTGCCCTGTCCTCGTCGTGAAGAAATCACAAGCTGATGCTCTTGCTTGA

Protein Analysis

186

Amino Acids

19.9

Weight (kDa)

7.59

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 22 - 177 1.3e-28 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 191
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 3 cut(s) 320, 424, 542
AluI AGCT 3 cut(s) 320, 424, 542
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 1 cut(s) 371
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 224, 424
AspLEI GCGC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 82, 193
BalI TGGCCA 1 cut(s) 80
BanII GRGCYC 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 211, 411
BccI CCATC 1 cut(s) 67
BceAI ACGGC 2 cut(s) 208, 430
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 2 cut(s) 336, 428
BfoI RGCGCY 1 cut(s) 225
BglII AGATCT 1 cut(s) 327
BisI GCNGC 2 cut(s) 225, 425
BlsI GCNGC 2 cut(s) 226, 426
BmiI GGNNCC 1 cut(s) 58
BmsI GCATC 3 cut(s) 388, 426, 535
BpiI GAAGAC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMII CTCAG 2 cut(s) 191, 345
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 211, 411
BsgI GTGCAG 1 cut(s) 118
BshFI GGCC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 1 cut(s) 350
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 3 cut(s) 162, 327, 376
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 190, 346
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 371
BsrI ACTGG 1 cut(s) 252
BssMI GATC 3 cut(s) 162, 327, 376
BssNI GRCGYC 1 cut(s) 212
Bst4CI ACNGT 4 cut(s) 239, 309, 361, 494
Bst6I CTCTTC 1 cut(s) 277
BstACI GRCGYC 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 229, 440, 504
BstDEI CTNAG 2 cut(s) 177, 354
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 3 cut(s) 165, 330, 379
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 3 cut(s) 162, 327, 376
BstMWI GCNNNNNNNGC 2 cut(s) 56, 228
BstNSI RCATGY 1 cut(s) 235
BstV1I GCAGC 2 cut(s) 211, 411
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 2 cut(s) 327, 376
BstYI RGATCY 2 cut(s) 327, 376
BsuRI GGCC 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 3 cut(s) 229, 440, 504
CfoI GCGC 1 cut(s) 224
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 235
CviAII CATG 3 cut(s) 115, 172, 232
CviJI RGCY 8 cut(s) 59, 80, 176, 320, 389, 424, 448, 542
CviKI_1 RGCY 8 cut(s) 59, 80, 176, 320, 389, 424, 448, 542
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 177, 354
DpnI GATC 3 cut(s) 164, 329, 378
DpnII GATC 3 cut(s) 162, 327, 376
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 277
EarI CTCTTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 450
FaeI CATG 3 cut(s) 118, 175, 235
FaiI YATR 7 cut(s) 116, 146, 173, 233, 245, 392, 394
FatI CATG 3 cut(s) 114, 171, 231
FauNDI CATATG 1 cut(s) 392
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 2 cut(s) 225, 425
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 2 cut(s) 225, 425
FspBI CTAG 2 cut(s) 336, 428
GlaI GCGC 1 cut(s) 223
GluI GCNGC 2 cut(s) 225, 425
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 80
HgaI GACGC 1 cut(s) 201
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 3 cut(s) 118, 175, 235
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 3 cut(s) 13, 140, 181
HpaI GTTAAC 1 cut(s) 268
HphI GGTGA 2 cut(s) 82, 193
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 4 cut(s) 18, 180, 381, 452
Hpy188III TCNNGA 3 cut(s) 125, 257, 526
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 2 cut(s) 217, 527
HpyAV CCTTC 2 cut(s) 61, 214
HpyCH4III ACNGT 4 cut(s) 239, 309, 361, 494
HpyCH4V TGCA 2 cut(s) 50, 99
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 228
HpyF3I CTNAG 2 cut(s) 177, 354
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 3 cut(s) 118, 175, 235
HspAI GCGC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 162, 327, 376
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 3 cut(s) 242, 265, 528
Lsp1109I GCAGC 2 cut(s) 211, 411
LweI GCATC 3 cut(s) 388, 426, 535
MaeI CTAG 2 cut(s) 336, 428
MaeIII GTNAC 1 cut(s) 361
MalI GATC 3 cut(s) 164, 329, 378
MboI GATC 3 cut(s) 162, 327, 376
MboII GAAGA 6 cut(s) 38, 76, 160, 240, 264, 541
MfeI CAATTG 1 cut(s) 508
MflI RGATCY 2 cut(s) 327, 376
MhlI GDGCHC 1 cut(s) 450
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 2 cut(s) 468, 508
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 2 cut(s) 134, 175
MmeI TCCRAC 3 cut(s) 213, 291, 352
MnlI CCTC 4 cut(s) 14, 143, 196, 530
Mox20I TGGCCA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 80
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 170
Msp20I TGGCCA 1 cut(s) 80
MunI CAATTG 1 cut(s) 508
MwoI GCNNNNNNNGC 2 cut(s) 56, 228
NdeI CATATG 1 cut(s) 392
NdeII GATC 3 cut(s) 162, 327, 376
NlaIII CATG 3 cut(s) 118, 175, 235
NlaIV GGNNCC 1 cut(s) 58
NmeAIII GCCGAG 3 cut(s) 85, 188, 198
NmuCI GTSAC 1 cut(s) 361
NspI RCATGY 1 cut(s) 235
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 226, 426
PleI GAGTC 2 cut(s) 134, 175
PpsI GAGTC 2 cut(s) 134, 175
PspN4I GGNNCC 1 cut(s) 58
PsuI RGATCY 2 cut(s) 327, 376
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 225, 425
Sau3AI GATC 3 cut(s) 162, 327, 376
SchI GAGTC 2 cut(s) 134, 175
SduI GDGCHC 1 cut(s) 450
SetI ASST 6 cut(s) 72, 135, 206, 322, 426, 544
SfaNI GCATC 3 cut(s) 388, 426, 535
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 2 cut(s) 468, 508
SspMI CTAG 2 cut(s) 336, 428
TaaI ACNGT 4 cut(s) 239, 309, 361, 494
TaqI TCGA 1 cut(s) 126
TasI AATT 2 cut(s) 468, 508
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 2 cut(s) 224, 424
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 1 cut(s) 160
TspRI CASTG 1 cut(s) 312
XceI RCATGY 1 cut(s) 235
XspI CTAG 2 cut(s) 336, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.