FvH4_4g31110

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002644
Physical Location & Seq
Reverse (-)
30393779 .. 30395213
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31110.t1

Sequence Viewer

Length: 573 bp
ATGAAGACAGCAGCAGCATCAGGACTAGTACTCGAGGGTAGCGAAGCCCACAAATCAATCCCAAAGGTGAGGGTGAGCAGAGTGCTATCTGTTCTCGATTTCATTCTGCGAGTAGTTGCAGCCCTCGGAACACTAGCAAGTGCCATTGCCATGGGAACCAGCAGGCAAACACTTCCCTTTGTCTCACAGTTTGCTCGATTCAAGGCTAATTACAAGGATCTTCCCACATTTACGTTCTTTGTGATTGTCAATTCAATTGTATGTGTCTACCTTGTGCTTTCACTTCCGGTGTCTATTGTCCACATAATCTGGACTGGAGCAGTGAAAAGTAGGATCATGTTGGTGATCTTCGACACGGCAATGATGGGTCTTCTAACTGCTGGAGCATCCTCAGCAACAGCAATAGTGTTCCTGGCACACTATGGCAGCTCAAGCGCCAATTGGTTTCCATTTTGCCGCCAATTTAACAACTTCTGTGAGCGCATTTCAGGTTCTCTCATCGGCTCTTTCGTCGCCGTTGTTGTGATTATACTGCTAATCATATTGTCTTCTGTCGCGATCTCTCGCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

191

Amino Acids

20.48

Weight (kDa)

10.29

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 27 - 175 4.5e-44 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 557
AciI CCGC 2 cut(s) 457, 568
AclWI GGATC 2 cut(s) 225, 341
AfaI GTAC 1 cut(s) 30
AgsI TTSAA 2 cut(s) 202, 255
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 1 cut(s) 429
AluI AGCT 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 2 cut(s) 225, 341
Ama87I CYCGRG 1 cut(s) 32
ApeKI GCWGC 4 cut(s) 11, 14, 119, 426
AspLEI GCGC 2 cut(s) 437, 483
AsuHPI GGTGA 3 cut(s) 79, 85, 355
AvaI CYCGRG 1 cut(s) 32
BbsI GAAGAC 3 cut(s) 11, 362, 540
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 4 cut(s) 23, 26, 131, 438
BccI CCATC 1 cut(s) 358
BceAI ACGGC 2 cut(s) 372, 500
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 187
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 3 cut(s) 26, 134, 571
BfoI RGCGCY 1 cut(s) 438
BisI GCNGC 6 cut(s) 12, 15, 120, 427, 457, 568
BlsI GCNGC 6 cut(s) 13, 16, 121, 428, 458, 569
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 26, 395
BpiI GAAGAC 3 cut(s) 11, 362, 540
BpmI CTGGAG 2 cut(s) 336, 402
Bpu10I CCTNAGC 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 415
BsaJI CCNNGG 2 cut(s) 124, 150
BsaWI WCCGGW 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 2 cut(s) 144, 366
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 124, 150
BseGI GGATG 1 cut(s) 386
BseMI GCAATG 2 cut(s) 144, 366
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 319
BseXI GCAGC 4 cut(s) 23, 26, 131, 438
Bsh1236I CGCG 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 4 cut(s) 217, 333, 345, 558
Bsp19I CCATGG 1 cut(s) 150
Bsp68I TCGCGA 1 cut(s) 557
BspACI CCGC 2 cut(s) 457, 568
BspCNI CTCAG 1 cut(s) 404
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 2 cut(s) 225, 341
BsrDI GCAATG 2 cut(s) 144, 366
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 124, 150
BssMI GATC 4 cut(s) 217, 333, 345, 558
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 1 cut(s) 391
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 386
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 2 cut(s) 437, 483
BstKTI GATC 4 cut(s) 220, 336, 348, 561
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 4 cut(s) 217, 333, 345, 558
BstMWI GCNNNNNNNGC 2 cut(s) 392, 432
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 411
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 4 cut(s) 23, 26, 131, 438
BstV2I GAAGAC 3 cut(s) 11, 362, 540
BstX2I RGATCY 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
BtuMI TCGCGA 1 cut(s) 557
Cac8I GCNNGC 1 cut(s) 164
CfoI GCGC 2 cut(s) 437, 483
Csp6I GTAC 1 cut(s) 29
CviAII CATG 2 cut(s) 151, 337
CviJI RGCY 5 cut(s) 47, 122, 206, 429, 504
CviKI_1 RGCY 5 cut(s) 47, 122, 206, 429, 504
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 1 cut(s) 391
DpnI GATC 4 cut(s) 219, 335, 347, 560
DpnII GATC 4 cut(s) 217, 333, 345, 558
Eco130I CCWWGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 154, 340
FaiI YATR 7 cut(s) 152, 262, 305, 338, 423, 530, 542
FatI CATG 2 cut(s) 150, 336
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 6 cut(s) 12, 15, 120, 427, 457, 568
FokI GGATG 1 cut(s) 373
Fsp4HI GCNGC 6 cut(s) 12, 15, 120, 427, 457, 568
FspBI CTAG 3 cut(s) 26, 134, 571
GlaI GCGC 2 cut(s) 436, 482
GluI GCNGC 6 cut(s) 12, 15, 120, 427, 457, 568
GsuI CTGGAG 2 cut(s) 336, 402
HaeII RGCGCY 1 cut(s) 438
HapII CCGG 1 cut(s) 287
HhaI GCGC 2 cut(s) 437, 483
Hin1II CATG 2 cut(s) 154, 340
Hin6I GCGC 2 cut(s) 435, 481
HinP1I GCGC 2 cut(s) 435, 481
HinfI GANTC 1 cut(s) 198
HpaII CCGG 1 cut(s) 287
HphI GGTGA 3 cut(s) 79, 85, 355
Hpy166II GTNNAC 2 cut(s) 268, 301
Hpy188I TCNGA 1 cut(s) 128
Hpy188III TCNNGA 4 cut(s) 21, 95, 310, 556
Hpy8I GTNNAC 2 cut(s) 268, 301
Hpy99I CGWCG 1 cut(s) 515
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 1 cut(s) 119
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 432
HpyF3I CTNAG 1 cut(s) 391
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 2 cut(s) 154, 340
HspAI GCGC 2 cut(s) 435, 481
Kzo9I GATC 4 cut(s) 217, 333, 345, 558
LmnI GCTCC 2 cut(s) 317, 383
Lsp1109I GCAGC 4 cut(s) 23, 26, 131, 438
LweI GCATC 2 cut(s) 26, 395
MaeI CTAG 3 cut(s) 26, 134, 571
MaeII ACGT 1 cut(s) 233
MalI GATC 4 cut(s) 219, 335, 347, 560
MboI GATC 4 cut(s) 217, 333, 345, 558
MboII GAAGA 5 cut(s) 16, 212, 340, 362, 540
MfeI CAATTG 2 cut(s) 255, 439
MflI RGATCY 1 cut(s) 217
MluCI AATT 5 cut(s) 208, 250, 255, 439, 461
MnlI CCTC 4 cut(s) 28, 63, 134, 400
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 3 cut(s) 149, 341, 359
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 413
MunI CAATTG 2 cut(s) 255, 439
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 2 cut(s) 392, 432
NcoI CCATGG 1 cut(s) 150
NdeII GATC 4 cut(s) 217, 333, 345, 558
NlaIII CATG 2 cut(s) 154, 340
NlaIV GGNNCC 1 cut(s) 157
NruI TCGCGA 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 32
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 507
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 6 cut(s) 13, 16, 121, 428, 458, 569
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 157
PspXI VCTCGAGB 1 cut(s) 32
PsuI RGATCY 1 cut(s) 217
RruI TCGCGA 1 cut(s) 557
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 3 cut(s) 149, 341, 359
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 6 cut(s) 12, 15, 120, 427, 457, 568
Sau3AI GATC 4 cut(s) 217, 333, 345, 558
ScaI AGTACT 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 413
SetI ASST 5 cut(s) 69, 236, 273, 431, 493
SfaNI GCATC 2 cut(s) 26, 395
Sfr274I CTCGAG 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 3 cut(s) 149, 341, 359
SmlI CTYRAG 2 cut(s) 32, 430
SmoI CTYRAG 2 cut(s) 32, 430
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 5 cut(s) 208, 250, 255, 439, 461
SsiI CCGC 2 cut(s) 457, 568
SspMI CTAG 3 cut(s) 26, 134, 571
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 189
TaiI ACGT 1 cut(s) 236
TaqI TCGA 4 cut(s) 33, 96, 196, 351
TasI AATT 5 cut(s) 208, 250, 255, 439, 461
TatI WGTACW 1 cut(s) 28
TauI GCSGC 2 cut(s) 459, 570
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 327
TseI GCWGC 4 cut(s) 11, 14, 119, 426
TspDTI ATGAA 2 cut(s) 17, 91
TspRI CASTG 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 3 cut(s) 26, 134, 571
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.