Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G15290 |
| fragaria_vesca | FvH4_4g31110 FvH4_6g48140 |
| malus_domestica | MD09G1053500.v1.1 MD13G1069600.v1.1 MD13G1069700.v1.1 MD16G1070000.v1.1 |
| prunus_persica | Prupe.1G277100_v2.0.a1 Prupe.1G277100_v2.0.a1 Prupe.3G264800_v2.0.a1 Prupe.3G264900_v2.0.a1 |
| pyrus_communis | pycom13g06250 pycom16g06100 |
| rosa_chinensis | RchiOBHm_Chr2g0167481 RchiOBHm_Chr4g0439491 |
| rosa_laevigata | RLG00000006266 RLG00000021723 |
| rosa_multiflora | Rmu_sc0001206.1_g000035 Rmu_sc0007276.1_g000008 Rmu_sc0043018.1_g000002 |
| rosa_roxburghii | Rroxscaffold_2G00083450 Rroxscaffold_2G00083460 Rroxscaffold_5G00380400 |
| rosa_rugosa | Rorug02G0530500 Rorug04G0318700 Rorug04G0318800 |
| rosa_samantha | Rh2AG597700 Rh2AG597800 Rh2BG609200 Rh2BG609300 Rh2CG580100 Rh2CG580200 Rh2DG620500 Rh2DG620600 Rh4AG370000 Rh4BG382000 Rh4CG396600 Rh4DG376700 |
| rosa_wichuraiana | Rw2G049740 Rw4G031550 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 267 |
| AccII | CGCG | 1 cut(s) | 185 |
| AciI | CCGC | 2 cut(s) | 109, 183 |
| AclI | AACGTT | 1 cut(s) | 233 |
| AclWI | GGATC | 2 cut(s) | 225, 341 |
| AcuI | CTGAAG | 1 cut(s) | 372 |
| AfaI | GTAC | 3 cut(s) | 410, 525, 531 |
| AgsI | TTSAA | 1 cut(s) | 466 |
| AjnI | CCWGG | 1 cut(s) | 411 |
| AluBI | AGCT | 3 cut(s) | 149, 386, 471 |
| AluI | AGCT | 3 cut(s) | 149, 386, 471 |
| Alw21I | GWGCWC | 1 cut(s) | 87 |
| Alw26I | GTCTC | 1 cut(s) | 498 |
| AlwI | GGATC | 2 cut(s) | 225, 341 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 474 |
| ApeKI | GCWGC | 5 cut(s) | 119, 317, 392, 395, 398 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 171 |
| AspLEI | GCGC | 2 cut(s) | 187, 483 |
| AsuC2I | CCSGG | 1 cut(s) | 164 |
| AsuHPI | GGTGA | 3 cut(s) | 85, 172, 370 |
| BanII | GRGCYC | 1 cut(s) | 208 |
| Bbv12I | GWGCWC | 1 cut(s) | 87 |
| BbvI | GCAGC | 5 cut(s) | 131, 329, 404, 407, 410 |
| BccI | CCATC | 2 cut(s) | 59, 358 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 176, 210 |
| BciT130I | CCWGG | 1 cut(s) | 413 |
| BclI | TGATCA | 1 cut(s) | 342 |
| BcnI | CCSGG | 1 cut(s) | 164 |
| BcoDI | GTCTC | 1 cut(s) | 498 |
| BfaI | CTAG | 1 cut(s) | 95 |
| BfmI | CTRYAG | 1 cut(s) | 315 |
| BisI | GCNGC | 5 cut(s) | 120, 318, 393, 396, 399 |
| BlsI | GCNGC | 5 cut(s) | 121, 319, 394, 397, 400 |
| Bme1390I | CCNGG | 2 cut(s) | 164, 413 |
| BmiI | GGNNCC | 2 cut(s) | 157, 505 |
| BmrFI | CCNGG | 2 cut(s) | 164, 413 |
| BmsI | GCATC | 1 cut(s) | 13 |
| BpmI | CTGGAG | 2 cut(s) | 402, 415 |
| BpuMI | CCSGG | 1 cut(s) | 164 |
| BsaI | GGTCTC | 1 cut(s) | 498 |
| BsaJI | CCNNGG | 1 cut(s) | 124 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 33, 516, 546 |
| Bse118I | RCCGGY | 1 cut(s) | 456 |
| Bse1I | ACTGG | 1 cut(s) | 446 |
| BseBI | CCWGG | 1 cut(s) | 413 |
| BseDI | CCNNGG | 1 cut(s) | 124 |
| BseGI | GGATG | 1 cut(s) | 51 |
| BseNI | ACTGG | 1 cut(s) | 446 |
| BseRI | GAGGAG | 1 cut(s) | 27 |
| BseXI | GCAGC | 5 cut(s) | 131, 329, 404, 407, 410 |
| Bsh1236I | CGCG | 1 cut(s) | 185 |
| BsiHKAI | GWGCWC | 1 cut(s) | 87 |
| BsiSI | CCGG | 2 cut(s) | 163, 457 |
| BsmAI | GTCTC | 1 cut(s) | 498 |
| Bso31I | GGTCTC | 1 cut(s) | 498 |
| Bsp1286I | GDGCHC | 2 cut(s) | 87, 208 |
| Bsp143I | GATC | 3 cut(s) | 217, 333, 342 |
| BspACI | CCGC | 2 cut(s) | 109, 183 |
| BspFNI | CGCG | 1 cut(s) | 185 |
| BspLI | GGNNCC | 2 cut(s) | 157, 505 |
| BspMAI | CTGCAG | 1 cut(s) | 319 |
| BspPI | GGATC | 2 cut(s) | 225, 341 |
| BspTNI | GGTCTC | 1 cut(s) | 498 |
| BsrFI | RCCGGY | 1 cut(s) | 456 |
| BsrI | ACTGG | 1 cut(s) | 446 |
| BssAI | RCCGGY | 1 cut(s) | 456 |
| BssECI | CCNNGG | 1 cut(s) | 124 |
| BssMI | GATC | 3 cut(s) | 217, 333, 342 |
| BssT1I | CCWWGG | 1 cut(s) | 124 |
| Bst2UI | CCWGG | 1 cut(s) | 413 |
| Bst4CI | ACNGT | 2 cut(s) | 358, 523 |
| Bst6I | CTCTTC | 1 cut(s) | 288 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 23 |
| BstC8I | GCNNGC | 2 cut(s) | 458, 479 |
| BstF5I | GGATG | 1 cut(s) | 51 |
| BstFNI | CGCG | 1 cut(s) | 185 |
| BstHHI | GCGC | 2 cut(s) | 187, 483 |
| BstKTI | GATC | 3 cut(s) | 220, 336, 345 |
| BstMAI | GTCTC | 1 cut(s) | 498 |
| BstMBI | GATC | 3 cut(s) | 217, 333, 342 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 14, 23, 392, 398, 432 |
| BstNI | CCWGG | 1 cut(s) | 413 |
| BstSCI | CCNGG | 2 cut(s) | 162, 411 |
| BstSFI | CTRYAG | 1 cut(s) | 315 |
| BstUI | CGCG | 1 cut(s) | 185 |
| BstV1I | GCAGC | 5 cut(s) | 131, 329, 404, 407, 410 |
| BstX2I | RGATCY | 1 cut(s) | 217 |
| BstYI | RGATCY | 1 cut(s) | 217 |
| BtsCI | GGATG | 1 cut(s) | 51 |
| Cac8I | GCNNGC | 2 cut(s) | 458, 479 |
| CaiI | CAGNNNCTG | 1 cut(s) | 474 |
| CfoI | GCGC | 2 cut(s) | 187, 483 |
| Cfr10I | RCCGGY | 1 cut(s) | 456 |
| Csp6I | GTAC | 3 cut(s) | 409, 524, 530 |
| CviAII | CATG | 1 cut(s) | 544 |
| CviQI | GTAC | 3 cut(s) | 409, 524, 530 |
| DpnI | GATC | 3 cut(s) | 219, 335, 344 |
| DpnII | GATC | 3 cut(s) | 217, 333, 342 |
| Eam1104I | CTCTTC | 1 cut(s) | 288 |
| EarI | CTCTTC | 1 cut(s) | 288 |
| Eco130I | CCWWGG | 1 cut(s) | 124 |
| Eco24I | GRGCYC | 1 cut(s) | 208 |
| Eco31I | GGTCTC | 1 cut(s) | 498 |
| Eco57I | CTGAAG | 1 cut(s) | 372 |
| EcoRII | CCWGG | 1 cut(s) | 411 |
| EcoT14I | CCWWGG | 1 cut(s) | 124 |
| EcoT38I | GRGCYC | 1 cut(s) | 208 |
| ErhI | CCWWGG | 1 cut(s) | 124 |
| FaeI | CATG | 1 cut(s) | 547 |
| FaiI | YATR | 7 cut(s) | 152, 262, 303, 404, 423, 518, 545 |
| FatI | CATG | 1 cut(s) | 543 |
| FauI | CCCGC | 1 cut(s) | 102 |
| FbaI | TGATCA | 1 cut(s) | 342 |
| FblI | GTMKAC | 1 cut(s) | 267 |
| Fnu4HI | GCNGC | 5 cut(s) | 120, 318, 393, 396, 399 |
| FokI | GGATG | 1 cut(s) | 38 |
| FriOI | GRGCYC | 1 cut(s) | 208 |
| Fsp4HI | GCNGC | 5 cut(s) | 120, 318, 393, 396, 399 |
| FspBI | CTAG | 1 cut(s) | 95 |
| GlaI | GCGC | 2 cut(s) | 186, 482 |
| GluI | GCNGC | 5 cut(s) | 120, 318, 393, 396, 399 |
| GsuI | CTGGAG | 2 cut(s) | 402, 415 |
| HapII | CCGG | 2 cut(s) | 163, 457 |
| HhaI | GCGC | 2 cut(s) | 187, 483 |
| Hin1II | CATG | 1 cut(s) | 547 |
| Hin6I | GCGC | 2 cut(s) | 185, 481 |
| HinP1I | GCGC | 2 cut(s) | 185, 481 |
| HincII | GTYRAC | 1 cut(s) | 231 |
| HindII | GTYRAC | 1 cut(s) | 231 |
| HinfI | GANTC | 1 cut(s) | 198 |
| HpaI | GTTAAC | 1 cut(s) | 231 |
| HpaII | CCGG | 2 cut(s) | 163, 457 |
| HphI | GGTGA | 3 cut(s) | 85, 172, 370 |
| Hpy166II | GTNNAC | 3 cut(s) | 231, 268, 409 |
| Hpy188I | TCNGA | 1 cut(s) | 352 |
| Hpy188III | TCNNGA | 4 cut(s) | 95, 310, 340, 564 |
| Hpy8I | GTNNAC | 3 cut(s) | 231, 268, 409 |
| Hpy99I | CGWCG | 1 cut(s) | 570 |
| HpyAV | CCTTC | 1 cut(s) | 187 |
| HpyCH4III | ACNGT | 2 cut(s) | 358, 523 |
| HpyCH4IV | ACGT | 3 cut(s) | 227, 233, 568 |
| HpyCH4V | TGCA | 4 cut(s) | 119, 143, 317, 449 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 14, 23, 392, 398, 432 |
| HpySE526I | ACGT | 3 cut(s) | 227, 233, 568 |
| Hsp92II | CATG | 1 cut(s) | 547 |
| HspAI | GCGC | 2 cut(s) | 185, 481 |
| KroI | GCCGGC | 1 cut(s) | 456 |
| KroNI | GCCGGC | 1 cut(s) | 458 |
| Ksp22I | TGATCA | 1 cut(s) | 342 |
| KspAI | GTTAAC | 1 cut(s) | 231 |
| Kzo9I | GATC | 3 cut(s) | 217, 333, 342 |
| LmnI | GCTCC | 3 cut(s) | 14, 383, 509 |
| Lsp1109I | GCAGC | 5 cut(s) | 131, 329, 404, 407, 410 |
| LweI | GCATC | 1 cut(s) | 13 |
| MaeI | CTAG | 1 cut(s) | 95 |
| MaeII | ACGT | 3 cut(s) | 227, 233, 568 |
| MaeIII | GTNAC | 1 cut(s) | 209 |
| MalI | GATC | 3 cut(s) | 219, 335, 344 |
| MboI | GATC | 3 cut(s) | 217, 333, 342 |
| MboII | GAAGA | 2 cut(s) | 212, 275 |
| MfeI | CAATTG | 1 cut(s) | 255 |
| MflI | RGATCY | 1 cut(s) | 217 |
| MhlI | GDGCHC | 2 cut(s) | 87, 208 |
| MluCI | AATT | 2 cut(s) | 255, 461 |
| MnlI | CCTC | 2 cut(s) | 5, 52 |
| MroNI | GCCGGC | 1 cut(s) | 456 |
| MroXI | GAANNNNTTC | 1 cut(s) | 171 |
| MseI | TTAA | 2 cut(s) | 230, 374 |
| MslI | CAYNNNNRTG | 1 cut(s) | 359 |
| MspI | CCGG | 2 cut(s) | 163, 457 |
| MspR9I | CCNGG | 2 cut(s) | 164, 413 |
| MunI | CAATTG | 1 cut(s) | 255 |
| MvaI | CCWGG | 1 cut(s) | 413 |
| MvnI | CGCG | 1 cut(s) | 185 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 14, 23, 392, 398, 432 |
| NaeI | GCCGGC | 1 cut(s) | 458 |
| NciI | CCSGG | 1 cut(s) | 164 |
| NdeII | GATC | 3 cut(s) | 217, 333, 342 |
| NgoMIV | GCCGGC | 1 cut(s) | 456 |
| NlaIII | CATG | 1 cut(s) | 547 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 505 |
| PdiI | GCCGGC | 1 cut(s) | 458 |
| PdmI | GAANNNNTTC | 1 cut(s) | 171 |
| PfeI | GAWTC | 1 cut(s) | 198 |
| PkrI | GCNGC | 5 cut(s) | 121, 319, 394, 397, 400 |
| Psp1406I | AACGTT | 1 cut(s) | 233 |
| Psp6I | CCWGG | 1 cut(s) | 411 |
| PspGI | CCWGG | 1 cut(s) | 411 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 505 |
| PstI | CTGCAG | 1 cut(s) | 319 |
| PstNI | CAGNNNCTG | 1 cut(s) | 474 |
| PsuI | RGATCY | 1 cut(s) | 217 |
| RsaI | GTAC | 3 cut(s) | 410, 525, 531 |
| RsaNI | GTAC | 3 cut(s) | 409, 524, 530 |
| RseI | CAYNNNNRTG | 1 cut(s) | 359 |
| SaqAI | TTAA | 2 cut(s) | 230, 374 |
| SatI | GCNGC | 5 cut(s) | 120, 318, 393, 396, 399 |
| Sau3AI | GATC | 3 cut(s) | 217, 333, 342 |
| ScrFI | CCNGG | 2 cut(s) | 164, 413 |
| SduI | GDGCHC | 2 cut(s) | 87, 208 |
| SetI | ASST | 8 cut(s) | 151, 230, 236, 273, 388, 414, 473, 571 |
| SfaNI | GCATC | 1 cut(s) | 13 |
| SfcI | CTRYAG | 1 cut(s) | 315 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 359 |
| Sse9I | AATT | 2 cut(s) | 255, 461 |
| SsiI | CCGC | 2 cut(s) | 109, 183 |
| SspMI | CTAG | 1 cut(s) | 95 |
| StyD4I | CCNGG | 2 cut(s) | 162, 411 |
| StyI | CCWWGG | 1 cut(s) | 124 |
| TaaI | ACNGT | 2 cut(s) | 358, 523 |
| TaiI | ACGT | 3 cut(s) | 230, 236, 571 |
| TaqI | TCGA | 2 cut(s) | 196, 565 |
| TasI | AATT | 2 cut(s) | 255, 461 |
| TatI | WGTACW | 2 cut(s) | 523, 529 |
| TfiI | GAWTC | 1 cut(s) | 198 |
| Tru1I | TTAA | 2 cut(s) | 230, 374 |
| Tru9I | TTAA | 2 cut(s) | 230, 374 |
| TseI | GCWGC | 5 cut(s) | 119, 317, 392, 395, 398 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XbaI | TCTAGA | 1 cut(s) | 94 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 409 |
| XmiI | GTMKAC | 1 cut(s) | 267 |
| XmnI | GAANNNNTTC | 1 cut(s) | 171 |
| XspI | CTAG | 1 cut(s) | 95 |