MD13G1069600.v1.1

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002644
Physical Location & Seq
Reverse (-)
4881720 .. 4882167
448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1069600.v1.1.491

Sequence Viewer

Length: 282 bp
ATGTCCATTGATTCACCTTTTATTCAATCTAAAGGGCAGAAAAAAAAAAAGGGAAAGAGTAAAGAGGAGAGAAGTGGCATGTTGAAATCACTTTTCTCCTGCTGTACAGCCATAGTGTACCTGGCGCACTACGGGAACCCCTCCACCAACTGGTTCGCATTCTGTCAGCAGTTCAACAGCTTCTGTGGGCGCATTTCCGGGTCTCTTATTGGCTCCTTCGTCGCCATAACTGTACTTGTACTGCTAATCATCATGTCATCCGCAGCAATTTCTCGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

94

Amino Acids

10.22

Weight (kDa)

9.9

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 32 - 77 9.1e-12 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 150
AciI CCGC 1 cut(s) 261
AfaI GTAC 4 cut(s) 106, 119, 234, 240
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 3 cut(s) 26, 85, 175
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 1 cut(s) 180
AluI AGCT 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 207
AlwNI CAGNNNCTG 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 127, 192
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 6
BbvI GCAGC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 122
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 207
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 122, 199
BmiI GGNNCC 2 cut(s) 137, 214
BmrFI CCNGG 2 cut(s) 122, 199
BpuMI CCSGG 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 207
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 1 cut(s) 207
BsmI GAATGC 1 cut(s) 158
Bso31I GGTCTC 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 104
BspACI CCGC 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 137, 214
BspTNI GGTCTC 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 104
BsrI ACTGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 232
BstAUI TGTACA 1 cut(s) 104
BstF5I GGATG 1 cut(s) 257
BstHHI GCGC 2 cut(s) 127, 192
BstMAI GTCTC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 120, 197
BstV1I GCAGC 1 cut(s) 275
BtsCI GGATG 1 cut(s) 257
CaiI CAGNNNCTG 1 cut(s) 183
CfoI GCGC 2 cut(s) 127, 192
Csp6I GTAC 4 cut(s) 105, 118, 233, 239
CviAII CATG 2 cut(s) 79, 253
CviJI RGCY 3 cut(s) 110, 180, 213
CviKI_1 RGCY 3 cut(s) 110, 180, 213
CviQI GTAC 4 cut(s) 105, 118, 233, 239
Eco31I GGTCTC 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 120
FaeI CATG 2 cut(s) 82, 256
FaiI YATR 4 cut(s) 80, 113, 227, 254
FatI CATG 2 cut(s) 78, 252
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 264
GlaI GCGC 2 cut(s) 126, 191
GluI GCNGC 1 cut(s) 264
HapII CCGG 1 cut(s) 198
HhaI GCGC 2 cut(s) 127, 192
Hin1II CATG 2 cut(s) 82, 256
Hin6I GCGC 2 cut(s) 125, 190
HinP1I GCGC 2 cut(s) 125, 190
HinfI GANTC 1 cut(s) 11
HpaII CCGG 1 cut(s) 198
HphI GGTGA 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 2 cut(s) 224, 279
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 277
HpySE526I ACGT 1 cut(s) 277
Hsp92II CATG 2 cut(s) 82, 256
HspAI GCGC 2 cut(s) 125, 190
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 5 cut(s) 107, 112, 134, 136, 211
Lsp1109I GCAGC 1 cut(s) 275
MaeII ACGT 1 cut(s) 277
MluCI AATT 1 cut(s) 267
MnlI CCTC 2 cut(s) 58, 151
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 2 cut(s) 122, 199
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 122
NciI CCSGG 1 cut(s) 199
NlaIII CATG 2 cut(s) 82, 256
NlaIV GGNNCC 2 cut(s) 137, 214
NspI RCATGY 1 cut(s) 82
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 11
PflMI CCANNNNNTGG 1 cut(s) 150
PkrI GCNGC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 137, 214
PstNI CAGNNNCTG 1 cut(s) 183
RsaI GTAC 4 cut(s) 106, 119, 234, 240
RsaNI GTAC 4 cut(s) 105, 118, 233, 239
SatI GCNGC 1 cut(s) 264
ScrFI CCNGG 2 cut(s) 122, 199
SetI ASST 4 cut(s) 19, 123, 182, 280
Sse9I AATT 1 cut(s) 267
SsiI CCGC 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 120, 197
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 274
TasI AATT 1 cut(s) 267
TatI WGTACW 3 cut(s) 104, 232, 238
TfiI GAWTC 1 cut(s) 11
TseI GCWGC 1 cut(s) 263
Van91I CCANNNNNTGG 1 cut(s) 150
XceI RCATGY 1 cut(s) 82
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.