Rmu_sc0007276.1_g000008

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007276.1
OG0002644
Physical Location & Seq
Reverse (-)
28298 .. 29056
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007276.1_g000008.1.cds

Sequence Viewer

Length: 579 bp
atgaaggcagcaacagtagcagcaggaccagaactacccgagggtagcgaagcccacaaaccaatcccaaaagtgagggtgagcagagtgctctctgttatcgatttcattctgcgagtagttgcagccgtcggaacactagcaagtgccatagccatgggaaccagcaggcaaacacttccctttgtcacacggtttgctcgattcaaggctaattacaaggatcttcccacgttcacgttctttgtgattgtcaattcaattgtatgtgtctaccttgtgctttcacttccggtgtctattgtccacataatctggactggagcagtgaaaagtaggatcaccttggtcatcttagacacggcaatgctgggtcttttaactgctggtgcatcctcagcaacagcaatagtggccctggcacactatggcagcaccgctgtcaattggtttccattctgccgccaattcaacaacttctgtcagcgcatttcaggctctctgatcggctcgttcgtcgccattgttgtgatcatactgctaatcatcttgtcctctgtcgcaatctctcgccgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

192

Amino Acids

20.59

Weight (kDa)

10.48

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AciI CCGC 3 cut(s) 438, 463, 574
AclWI GGATC 2 cut(s) 231, 347
AgsI TTSAA 3 cut(s) 208, 261, 472
AjnI CCWGG 1 cut(s) 417
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 2 cut(s) 231, 347
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 4 cut(s) 8, 20, 125, 432
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 2 cut(s) 26, 415
AsuHPI GGTGA 2 cut(s) 91, 334
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 397
BbvI GCAGC 4 cut(s) 20, 32, 137, 444
BceAI ACGGC 2 cut(s) 113, 378
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 1 cut(s) 531
BfaI CTAG 2 cut(s) 140, 577
BisI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
BlsI GCNGC 6 cut(s) 10, 22, 127, 434, 464, 575
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 26, 415
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 397
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 39, 156, 345, 417
BsaWI WCCGGW 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 4 cut(s) 39, 156, 345, 417
BseGI GGATG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 325
BseXI GCAGC 4 cut(s) 20, 32, 137, 444
BseYI CCCAGC 1 cut(s) 370
BshFI GGCC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 293
BsnI GGCC 1 cut(s) 416
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 4 cut(s) 223, 339, 504, 531
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 3 cut(s) 438, 463, 574
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 1 cut(s) 410
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 2 cut(s) 231, 347
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 39, 156, 345, 417
BssMI GATC 4 cut(s) 223, 339, 504, 531
BssT1I CCWWGG 2 cut(s) 156, 345
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 16, 195
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 355, 397
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 4 cut(s) 226, 342, 507, 534
BstMBI GATC 4 cut(s) 223, 339, 504, 531
BstMWI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 417
BstV1I GCAGC 4 cut(s) 20, 32, 137, 444
BstX2I RGATCY 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 1 cut(s) 416
BsuTUI ATCGAT 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 392
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 170
CfoI GCGC 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 26, 415
ClaI ATCGAT 1 cut(s) 102
CviAII CATG 1 cut(s) 157
CviJI RGCY 7 cut(s) 53, 128, 155, 212, 416, 498, 510
CviKI_1 RGCY 7 cut(s) 53, 128, 155, 212, 416, 498, 510
DdeI CTNAG 2 cut(s) 355, 397
DpnI GATC 4 cut(s) 225, 341, 506, 533
DpnII GATC 4 cut(s) 223, 339, 504, 531
Eco130I CCWWGG 2 cut(s) 156, 345
Eco47I GGWCC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 2 cut(s) 156, 345
ErhI CCWWGG 2 cut(s) 156, 345
FaeI CATG 1 cut(s) 160
FaiI YATR 6 cut(s) 152, 158, 268, 311, 429, 536
FatI CATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 531
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
FokI GGATG 1 cut(s) 379
Fsp4HI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
FspBI CTAG 2 cut(s) 140, 577
GlaI GCGC 1 cut(s) 488
GluI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
GsaI CCCAGC 1 cut(s) 374
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 489
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HinfI GANTC 1 cut(s) 204
HpaII CCGG 1 cut(s) 293
HphI GGTGA 2 cut(s) 91, 334
Hpy166II GTNNAC 3 cut(s) 237, 274, 307
Hpy188I TCNGA 2 cut(s) 134, 504
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 3 cut(s) 237, 274, 307
Hpy99I CGWCG 2 cut(s) 134, 521
HpyCH4III ACNGT 2 cut(s) 16, 195
HpyCH4IV ACGT 2 cut(s) 233, 239
HpyCH4V TGCA 2 cut(s) 125, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
HpyF3I CTNAG 2 cut(s) 355, 397
HpySE526I ACGT 2 cut(s) 233, 239
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 487
Ksp22I TGATCA 1 cut(s) 531
Kzo9I GATC 4 cut(s) 223, 339, 504, 531
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 4 cut(s) 20, 32, 137, 444
LweI GCATC 1 cut(s) 401
MaeI CTAG 2 cut(s) 140, 577
MaeII ACGT 2 cut(s) 233, 239
MaeIII GTNAC 1 cut(s) 187
MalI GATC 4 cut(s) 225, 341, 506, 533
MboI GATC 4 cut(s) 223, 339, 504, 531
MboII GAAGA 1 cut(s) 218
MfeI CAATTG 2 cut(s) 261, 445
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 5 cut(s) 214, 256, 261, 445, 467
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 4 cut(s) 34, 69, 406, 565
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 3 cut(s) 155, 365, 527
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 2 cut(s) 261, 445
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 4 cut(s) 17, 398, 413, 495
NcoI CCATGG 1 cut(s) 156
NdeII GATC 4 cut(s) 223, 339, 504, 531
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 187
PcsI WCGNNNNNNNCGW 2 cut(s) 199, 513
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 6 cut(s) 10, 22, 127, 434, 464, 575
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 370
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 2 cut(s) 26, 415
PsuI RGATCY 1 cut(s) 223
RseI CAYNNNNRTG 3 cut(s) 155, 365, 527
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 6 cut(s) 9, 21, 126, 433, 463, 574
Sau3AI GATC 4 cut(s) 223, 339, 504, 531
Sau96I GGNCC 2 cut(s) 26, 415
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 1 cut(s) 93
SetI ASST 4 cut(s) 236, 242, 279, 347
SfaNI GCATC 1 cut(s) 401
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 3 cut(s) 155, 365, 527
Sse9I AATT 5 cut(s) 214, 256, 261, 445, 467
SsiI CCGC 3 cut(s) 438, 463, 574
SspMI CTAG 2 cut(s) 140, 577
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 156, 345
TaaI ACNGT 2 cut(s) 16, 195
TaiI ACGT 2 cut(s) 236, 242
TaqI TCGA 2 cut(s) 102, 202
TasI AATT 5 cut(s) 214, 256, 261, 445, 467
TauI GCSGC 2 cut(s) 465, 576
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 4 cut(s) 8, 20, 125, 432
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 17, 97
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 2 cut(s) 140, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.