pycom13g06250

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002644
Physical Location & Seq
Reverse (-)
4052676 .. 4052888
213 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06250.1

Sequence Viewer

Length: 213 bp
ATGATGGGGCTTGTAACTGCCGGAGCTTCAGCTGCTACAGCCATAGTGTACCTGGCGCACTACGGGAACCCCTCCACCAACTGGTTCGCTTTCTGTCAGCAGTTCAACAGCTTCTGTGAACGCATTTCCGGGTCTCTCATTGGCTCCTTCGTCGCCATAACTGTACTTGTATTGCTAATCATCATGTCATCCGCGGCAATTTCTCGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

71

Amino Acids

7.45

Weight (kDa)

8.89

Isoelectric Point (pI)

26.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 1 - 54 1.1e-17 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 81
AccII CGCG 1 cut(s) 194
AciI CCGC 2 cut(s) 192, 194
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 2 cut(s) 50, 165
AfiI CCNNNNNNNGG 1 cut(s) 81
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 3 cut(s) 26, 32, 111
AluI AGCT 3 cut(s) 26, 32, 111
Alw26I GTCTC 1 cut(s) 138
AlwNI CAGNNNCTG 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 58
AsuC2I CCSGG 1 cut(s) 130
BbvI GCAGC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 2 cut(s) 33, 195
BlsI GCNGC 2 cut(s) 34, 196
Bme1390I CCNGG 2 cut(s) 53, 130
BmiI GGNNCC 2 cut(s) 68, 145
BmrFI CCNGG 2 cut(s) 53, 130
BpuMI CCSGG 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse1I ACTGG 1 cut(s) 86
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 194
BsiSI CCGG 2 cut(s) 21, 129
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 138
BspACI CCGC 2 cut(s) 192, 194
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 68, 145
BspTNI GGTCTC 1 cut(s) 138
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 163
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 188
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 58
BstMAI GTCTC 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 32, 38
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 51, 128
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 19
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 188
CaiI CAGNNNCTG 1 cut(s) 114
CfoI GCGC 1 cut(s) 58
Cfr42I CCGCGG 1 cut(s) 195
Csp6I GTAC 2 cut(s) 49, 164
CviAII CATG 1 cut(s) 184
CviJI RGCY 6 cut(s) 10, 26, 32, 41, 111, 144
CviKI_1 RGCY 6 cut(s) 10, 26, 32, 41, 111, 144
CviQI GTAC 2 cut(s) 49, 164
Eco31I GGTCTC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 51
FaeI CATG 1 cut(s) 187
FaiI YATR 3 cut(s) 44, 158, 185
FatI CATG 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 33, 195
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 33, 195
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 33, 195
HapII CCGG 2 cut(s) 21, 129
HhaI GCGC 1 cut(s) 58
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HpaII CCGG 2 cut(s) 21, 129
Hpy166II GTNNAC 2 cut(s) 49, 119
Hpy188III TCNNGA 1 cut(s) 204
Hpy8I GTNNAC 2 cut(s) 49, 119
Hpy99I CGWCG 2 cut(s) 155, 210
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 208
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 38
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 56
KspI CCGCGG 1 cut(s) 195
LmnI GCTCC 2 cut(s) 23, 149
LpnPI CCDG 5 cut(s) 34, 38, 65, 67, 142
Lsp1109I GCAGC 1 cut(s) 19
MaeII ACGT 1 cut(s) 208
MaeIII GTNAC 1 cut(s) 13
MluCI AATT 1 cut(s) 198
MnlI CCTC 1 cut(s) 82
MspA1I CMGCKG 2 cut(s) 32, 194
MspI CCGG 2 cut(s) 21, 129
MspR9I CCNGG 2 cut(s) 53, 130
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 2 cut(s) 32, 38
NciI CCSGG 1 cut(s) 130
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 68, 145
PflMI CCANNNNNTGG 1 cut(s) 81
PkrI GCNGC 2 cut(s) 34, 196
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 68, 145
PstNI CAGNNNCTG 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 2 cut(s) 50, 165
RsaNI GTAC 2 cut(s) 49, 164
SacII CCGCGG 1 cut(s) 195
SatI GCNGC 2 cut(s) 33, 195
ScrFI CCNGG 2 cut(s) 53, 130
SetI ASST 5 cut(s) 28, 34, 54, 113, 211
SfcI CTRYAG 1 cut(s) 36
Sfr303I CCGCGG 1 cut(s) 195
SgrBI CCGCGG 1 cut(s) 195
Sse9I AATT 1 cut(s) 198
SsiI CCGC 2 cut(s) 192, 194
StyD4I CCNGG 2 cut(s) 51, 128
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 205
TasI AATT 1 cut(s) 198
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 197
TseI GCWGC 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.