Rw2G049740

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002644
Physical Location & Seq
Reverse (-)
77356580 .. 77357872
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049740.1

Sequence Viewer

Length: 567 bp
ATGAAGGGAGCTGCAGCAGCTATTGAGCTTGGTGAGTCCAAAATCGGTTCAACACCAAGAAGCGGGGTGAACAGAGGGGCAGCCATCCTCGACTTCATTCTAAGGATAATAGCGTTTCTTGGTACCTTAGTGAGTGCTATAGCTATGGGAACAACCAGGGAAAGACTTCCTTTTTTCACACAGTTCCTTCAGTTTAGGGCTGAGTATAATGATCTTCCAACATTTACGTTCTTTGTGGTTGCCAATTCCATCGTATGTGCCTATCTGATGTTTTCGCTTGCTCTTTCTATCTTCCACATCATAAGAAGTAATGCAAAGAACAGTAGAATAATCTTGATCTTCTTCGACACGGGAATGCTGGCTCTTCTGACAGCGGGTGCCTCGGCCGCCACAGCTATTGTGTACTTAGCACACAAGGGGAATGCCAAGGCAAACTGGTTCCCAATCTGTCAACAATTCAATTCCTTCTGCGAACGAATCTCTGGCTCTTTGATTGGATCTTTCGTGGCAATTGTTATTTTCGTTCTCTTGATCTTAATGTCAGCTGCCGCCCTTTCTCGGCGTTGA

Protein Analysis

188

Amino Acids

20.4

Weight (kDa)

10.03

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 25 - 172 5.6e-48 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 122, 377
AciI CCGC 4 cut(s) 63, 374, 387, 549
AclWI GGATC 1 cut(s) 505
AcoI YGGCCR 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 2 cut(s) 124, 404
AfiI CCNNNNNNNGG 2 cut(s) 62, 558
AgsI TTSAA 2 cut(s) 51, 460
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 6 cut(s) 11, 20, 28, 143, 395, 545
AluI AGCT 6 cut(s) 11, 20, 28, 143, 395, 545
AlwI GGATC 1 cut(s) 505
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 5 cut(s) 11, 14, 17, 80, 545
Asp700I GAANNNNTTC 1 cut(s) 165
Asp718I GGTACC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 44, 79
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BanI GGYRCC 2 cut(s) 122, 377
BbvI GCAGC 4 cut(s) 26, 29, 92, 532
BccI CCATC 2 cut(s) 92, 257
BciT130I CCWGG 1 cut(s) 157
BfmI CTRYAG 2 cut(s) 12, 138
BisI GCNGC 7 cut(s) 12, 15, 18, 81, 387, 546, 549
BlsI GCNGC 7 cut(s) 13, 16, 19, 82, 388, 547, 550
Bme1390I CCNGG 1 cut(s) 157
BmiI GGNNCC 3 cut(s) 124, 379, 440
BmrFI CCNGG 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 156, 381, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 558
Bse1I ACTGG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 3 cut(s) 156, 381, 426
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 2 cut(s) 62, 558
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 440
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 26, 29, 92, 532
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 1 cut(s) 386
BshNI GGYRCC 2 cut(s) 122, 377
BsiEI CGRYCG 1 cut(s) 387
BslI CCNNNNNNNGG 2 cut(s) 62, 558
BsmI GAATGC 2 cut(s) 360, 427
BsnI GGCC 1 cut(s) 386
Bsp143I GATC 4 cut(s) 211, 336, 497, 531
BspACI CCGC 4 cut(s) 63, 374, 387, 549
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 3 cut(s) 124, 379, 440
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 505
BspQI GCTCTTC 1 cut(s) 369
BspT107I GGYRCC 2 cut(s) 122, 377
BsrI ACTGG 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 156, 381, 426
BssMI GATC 4 cut(s) 211, 336, 497, 531
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 2 cut(s) 183, 323
Bst6I CTCTTC 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 279, 360
BstDEI CTNAG 4 cut(s) 101, 127, 201, 406
BstF5I GGATG 1 cut(s) 84
BstKTI GATC 4 cut(s) 214, 339, 500, 534
BstMBI GATC 4 cut(s) 211, 336, 497, 531
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 3 cut(s) 17, 386, 392
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 2 cut(s) 12, 138
BstV1I GCAGC 4 cut(s) 26, 29, 92, 532
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BstZI CGGCCG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 386
BtsCI GGATG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 279, 360
Csp6I GTAC 2 cut(s) 123, 403
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviQI GTAC 2 cut(s) 123, 403
DdeI CTNAG 4 cut(s) 101, 127, 201, 406
DpnI GATC 4 cut(s) 213, 338, 499, 533
DpnII GATC 4 cut(s) 211, 336, 497, 531
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 426
Eco52I CGGCCG 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
FaiI YATR 5 cut(s) 140, 146, 207, 256, 302
FalI AAGNNNNNCTT 2 cut(s) 154, 186
FauI CCCGC 2 cut(s) 56, 367
Fnu4HI GCNGC 7 cut(s) 12, 15, 18, 81, 387, 546, 549
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 7 cut(s) 12, 15, 18, 81, 387, 546, 549
GluI GCNGC 7 cut(s) 12, 15, 18, 81, 387, 546, 549
HaeIII GGCC 1 cut(s) 386
HincII GTYRAC 1 cut(s) 452
HindII GTYRAC 1 cut(s) 452
HinfI GANTC 2 cut(s) 35, 477
HphI GGTGA 2 cut(s) 44, 79
Hpy166II GTNNAC 3 cut(s) 70, 403, 452
Hpy188I TCNGA 2 cut(s) 267, 369
Hpy188III TCNNGA 2 cut(s) 334, 529
Hpy8I GTNNAC 3 cut(s) 70, 403, 452
HpyAV CCTTC 2 cut(s) 197, 475
HpyCH4III ACNGT 2 cut(s) 183, 323
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 14, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 386, 392
HpyF3I CTNAG 4 cut(s) 101, 127, 201, 406
HpySE526I ACGT 1 cut(s) 227
KpnI GGTACC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 211, 336, 497, 531
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 8
LpnPI CCDG 5 cut(s) 142, 169, 344, 421, 468
Lsp1109I GCAGC 4 cut(s) 26, 29, 92, 532
MaeII ACGT 1 cut(s) 227
MalI GATC 4 cut(s) 213, 338, 499, 533
MboI GATC 4 cut(s) 211, 336, 497, 531
MboII GAAGA 5 cut(s) 206, 283, 331, 334, 356
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 497
MluCI AATT 4 cut(s) 244, 455, 460, 510
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 3 cut(s) 68, 98, 391
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 536
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 2 cut(s) 374, 545
MspR9I CCNGG 1 cut(s) 157
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 2 cut(s) 360, 427
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 3 cut(s) 17, 386, 392
NdeII GATC 4 cut(s) 211, 336, 497, 531
NlaIV GGNNCC 3 cut(s) 124, 379, 440
NmeAIII GCCGAG 2 cut(s) 362, 538
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 2 cut(s) 360, 427
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 7 cut(s) 13, 16, 19, 82, 388, 547, 550
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 3 cut(s) 124, 379, 440
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 124, 404
RsaNI GTAC 2 cut(s) 123, 403
RseI CAYNNNNRTG 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 7 cut(s) 12, 15, 18, 81, 387, 546, 549
Sau3AI GATC 4 cut(s) 211, 336, 497, 531
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 157
SetI ASST 8 cut(s) 13, 22, 30, 128, 145, 230, 397, 547
SfcI CTRYAG 2 cut(s) 12, 138
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 4 cut(s) 244, 455, 460, 510
SsiI CCGC 4 cut(s) 63, 374, 387, 549
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 183, 323
TaiI ACGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 90, 345
TasI AATT 4 cut(s) 244, 455, 460, 510
TatI WGTACW 1 cut(s) 402
TauI GCSGC 2 cut(s) 389, 551
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TseI GCWGC 5 cut(s) 11, 14, 17, 80, 545
TspDTI ATGAA 2 cut(s) 17, 85
XmnI GAANNNNTTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.