FvH4_4g31111

Belongs to the endosulfine family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006518
Physical Location & Seq
Forward (+)
30395845 .. 30399183
3339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31111.t1

Sequence Viewer

Length: 705 bp
ATGAGGCTCGAAACAATTCGGGCTGAATTAGCAGAAGCCTATGTTGCACGGACACGGACTCGGCGACATGACACTGCACGGCGCGACACGCCGATATGGCAAAACTCAAAAACTGAATATCCGATATGTCTCCAGGCTGCAGCTTCGTTCTCATATCTCTGCTCTGCAATTGGATTGGATCACCCCGTTCCGGCTCCGGTTTTCCCAGGCGGATCTTTCATCAGCTGTCACCCGTGCCTAGAGCAAAATATGTCGGATACAAACATGGAGGACGTGAAGGACCAAGAGCTTGTTCAAGGTCATGGTGACAATGCCGAGCAAGACGTGAAAGCTTCAGATGAAAGTCATGATGATCATCCCATGCCTTCAGTCCAGAAGGAGGAGGAAACAATTAAGAAAAAATATGGAGGCATTATTCCTAAGAAGCCTCCACTAATATCCAAGGACCATGAGCGGGCTTATTTTGATTCTGCTGATTGGGCATTGGGAAAGGGCACACATAAGCCGAAAGGACCACTCGAAGCACTCCGCCCAAAGCTGCAGCCCACACCACATCAGCAAGTGCGTTCCAGACGTGCCTCCTATGCGAGGGCAGATGATGTCGAAGATGGTGTCAATAACAACACTTCAGAGGAGGATATCCAAAGTGGCGTAACCGATGGCAGCACAAACACCGTGACTGAGGATCAGAGCCGTCATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

235

Amino Acids

26.12

Weight (kDa)

5.49

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 122 - 193 1.3e-23 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 454
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 210, 454, 529
AclWI GGATC 3 cut(s) 186, 220, 693
AcuI CTGAAG 3 cut(s) 318, 351, 612
AfiI CCNNNNNNNGG 4 cut(s) 190, 379, 454, 588
AgsI TTSAA 1 cut(s) 296
AjiI CACGTC 3 cut(s) 274, 325, 575
AjnI CCWGG 2 cut(s) 132, 205
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 5 cut(s) 143, 225, 289, 332, 538
AluI AGCT 5 cut(s) 143, 225, 289, 332, 538
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 3 cut(s) 186, 220, 693
ApeKI GCWGC 5 cut(s) 137, 140, 538, 541, 663
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 3 cut(s) 280, 445, 512
AsuHPI GGTGA 3 cut(s) 173, 221, 317
AvaII GGWCC 3 cut(s) 280, 445, 512
BaeGI GKGCMC 1 cut(s) 497
BbvI GCAGC 5 cut(s) 124, 152, 525, 553, 675
BccI CCATC 2 cut(s) 602, 653
BceAI ACGGC 2 cut(s) 95, 678
BciT130I CCWGG 2 cut(s) 134, 207
BciVI GTATCC 1 cut(s) 250
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 2 cut(s) 138, 539
BfuI GTATCC 1 cut(s) 250
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 5 cut(s) 138, 141, 539, 542, 664
BlsI GCNGC 5 cut(s) 139, 142, 540, 543, 665
Bme1390I CCNGG 2 cut(s) 134, 207
Bme18I GGWCC 3 cut(s) 280, 445, 512
BmgBI CACGTC 3 cut(s) 274, 325, 575
BmgT120I GGNCC 3 cut(s) 280, 445, 512
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 2 cut(s) 134, 207
BpmI CTGGAG 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 205, 441
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 379, 454, 588
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 2 cut(s) 134, 207
BseDI CCNNGG 2 cut(s) 205, 441
BseGI GGATG 1 cut(s) 355
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 4 cut(s) 190, 379, 454, 588
BseMII CTCAG 1 cut(s) 672
BseRI GAGGAG 2 cut(s) 395, 647
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 5 cut(s) 124, 152, 525, 553, 675
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 84
BsiSI CCGG 2 cut(s) 191, 197
BslI CCNNNNNNNGG 4 cut(s) 190, 379, 454, 588
BsmAI GTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 497
Bsp143I GATC 4 cut(s) 178, 212, 352, 685
BspACI CCGC 3 cut(s) 210, 454, 529
BspCNI CTCAG 1 cut(s) 673
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 2 cut(s) 346, 697
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 2 cut(s) 142, 543
BspPI GGATC 3 cut(s) 186, 220, 693
BsrBI CCGCTC 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 205, 441
BssMI GATC 4 cut(s) 178, 212, 352, 685
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 134, 207
Bst4CI ACNGT 1 cut(s) 676
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 420, 681
BstENI CCTNNNNNAGG 1 cut(s) 586
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 4 cut(s) 181, 215, 355, 688
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 4 cut(s) 178, 212, 352, 685
BstMWI GCNNNNNNNGC 6 cut(s) 29, 44, 88, 97, 479, 584
BstNI CCWGG 2 cut(s) 134, 207
BstSCI CCNGG 2 cut(s) 132, 205
BstSFI CTRYAG 2 cut(s) 138, 539
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 124, 152, 525, 553, 675
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuI GTATCC 1 cut(s) 250
BtrI CACGTC 3 cut(s) 274, 325, 575
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 456
CciI TCATGA 2 cut(s) 346, 697
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 3 cut(s) 280, 445, 512
CviAII CATG 7 cut(s) 68, 265, 302, 347, 361, 449, 698
DdeI CTNAG 2 cut(s) 420, 681
DpnI GATC 4 cut(s) 180, 214, 354, 687
DpnII GATC 4 cut(s) 178, 212, 352, 685
EciI GGCGGA 2 cut(s) 225, 518
Eco130I CCWWGG 1 cut(s) 441
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 3 cut(s) 280, 445, 512
Eco57I CTGAAG 3 cut(s) 318, 351, 612
EcoNI CCTNNNNNAGG 1 cut(s) 586
EcoRII CCWGG 2 cut(s) 132, 205
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 7 cut(s) 71, 268, 305, 350, 364, 452, 701
FatI CATG 7 cut(s) 67, 264, 301, 346, 360, 448, 697
FauI CCCGC 1 cut(s) 447
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 5 cut(s) 138, 141, 539, 542, 664
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 138, 141, 539, 542, 664
FspBI CTAG 1 cut(s) 239
GlaI GCGC 1 cut(s) 83
GluI GCNGC 5 cut(s) 138, 141, 539, 542, 664
GsuI CTGGAG 1 cut(s) 116
HapII CCGG 2 cut(s) 191, 197
HhaI GCGC 1 cut(s) 84
Hin1II CATG 7 cut(s) 71, 268, 305, 350, 364, 452, 701
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 2 cut(s) 58, 467
HpaII CCGG 2 cut(s) 191, 197
HphI GGTGA 3 cut(s) 173, 221, 317
Hpy188I TCNGA 5 cut(s) 123, 256, 337, 631, 690
Hpy188III TCNNGA 4 cut(s) 347, 373, 570, 698
HpyAV CCTTC 3 cut(s) 271, 370, 375
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 3 cut(s) 273, 324, 574
HpyCH4V TGCA 5 cut(s) 47, 77, 140, 167, 541
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 44, 88, 97, 479, 584
HpyF3I CTNAG 2 cut(s) 420, 681
HpySE526I ACGT 3 cut(s) 273, 324, 574
Hsp92II CATG 7 cut(s) 71, 268, 305, 350, 364, 452, 701
HspAI GCGC 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 4 cut(s) 178, 212, 352, 685
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 8 cut(s) 119, 146, 192, 204, 210, 219, 386, 583
Lsp1109I GCAGC 5 cut(s) 124, 152, 525, 553, 675
MaeI CTAG 1 cut(s) 239
MaeII ACGT 3 cut(s) 273, 324, 574
MaeIII GTNAC 4 cut(s) 227, 305, 652, 676
MalI GATC 4 cut(s) 180, 214, 354, 687
MbiI CCGCTC 1 cut(s) 454
MboI GATC 4 cut(s) 178, 212, 352, 685
MboII GAAGA 1 cut(s) 617
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 497
MluCI AATT 4 cut(s) 15, 26, 168, 390
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 2 cut(s) 191, 197
MspR9I CCNGG 2 cut(s) 134, 207
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 2 cut(s) 134, 207
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 6 cut(s) 29, 44, 88, 97, 479, 584
NdeII GATC 4 cut(s) 178, 212, 352, 685
NlaIII CATG 7 cut(s) 71, 268, 305, 350, 364, 452, 701
NlaIV GGNNCC 1 cut(s) 195
NmeAIII GCCGAG 2 cut(s) 40, 340
NmuCI GTSAC 3 cut(s) 227, 305, 676
PagI TCATGA 2 cut(s) 346, 697
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 5 cut(s) 139, 142, 540, 543, 665
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 132, 205
PspGI CCWGG 2 cut(s) 132, 205
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 3 cut(s) 280, 445, 512
PstI CTGCAG 2 cut(s) 142, 543
PsuI RGATCY 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 5 cut(s) 138, 141, 539, 542, 664
Sau3AI GATC 4 cut(s) 178, 212, 352, 685
Sau96I GGNCC 3 cut(s) 280, 445, 512
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 134, 207
SduI GDGCHC 1 cut(s) 497
SetI ASST 9 cut(s) 145, 227, 276, 291, 301, 327, 334, 540, 577
SfcI CTRYAG 2 cut(s) 138, 539
SinI GGWCC 3 cut(s) 280, 445, 512
Sse9I AATT 4 cut(s) 15, 26, 168, 390
SsiI CCGC 3 cut(s) 210, 454, 529
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 132, 205
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 3 cut(s) 276, 327, 577
TaqI TCGA 3 cut(s) 9, 519, 603
TasI AATT 4 cut(s) 15, 26, 168, 390
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 3 cut(s) 227, 305, 676
TseI GCWGC 5 cut(s) 137, 140, 538, 541, 663
Tsp45I GTSAC 3 cut(s) 227, 305, 676
TspDTI ATGAA 2 cut(s) 208, 354
TspGWI ACGGA 2 cut(s) 64, 70
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 280, 445, 512
XagI CCTNNNNNAGG 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.