MD13G1069900.v1.1

Belongs to the endosulfine family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006518
Physical Location & Seq
Forward (+)
4884636 .. 4887109
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1069900.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTCCGGTGCAAACATGGACGAGGTTAAGGAGCAAGAGGTTGCTGATAGCACTCCTATGGATCTAGGTGATGGTGACGATGCCGGGGGAGACCAAAAAGCTTCAAATGAAAATCATGGAAATCCCATGCCTTCAGCCCAACAGGAGGAGGCAGCAATTAAGAAAAAGTATGGAGGCATACTACCAAAGAAGACTCCCCTAATATCCAAGGACCATGAGCGCGCTTATTTTGATTCTGCTGATTGGGCATTGGGAAAGCAAGGAGCAAAGCCAAAAGGGCCACTTGAAGCACTCCGCCCAAAGCTGCAGCCCACACCACACCAGCAGGGTCGTTCAAGGCGCTCAGCTTATTCTCGTGGAGGCGAAGGTGATGATGGCGGAAATATGAACATTTCCTCAGAGGATCAACTAGATAGTGGCAGCGGTAACAACACTGGCTATGAGGATCAGATCCACCACCACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

156

Amino Acids

16.67

Weight (kDa)

5.1

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 44 - 116 3.5e-26 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 222
AciI CCGC 3 cut(s) 295, 378, 423
AclWI GGATC 4 cut(s) 69, 411, 445, 453
AcuI CTGAAG 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 3 cut(s) 105, 287, 336
AluBI AGCT 3 cut(s) 101, 304, 347
AluI AGCT 3 cut(s) 101, 304, 347
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 4 cut(s) 69, 411, 445, 453
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 4 cut(s) 152, 304, 307, 420
AspLEI GCGC 3 cut(s) 222, 224, 342
AspS9I GGNCC 2 cut(s) 211, 278
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 80, 86, 380
AvaII GGWCC 1 cut(s) 211
BauI CACGAG 2 cut(s) 354, 461
BbsI GAAGAC 1 cut(s) 197
BbvI GCAGC 4 cut(s) 164, 291, 319, 432
BccI CCATC 2 cut(s) 65, 368
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 2 cut(s) 65, 410
BfmI CTRYAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 277
BisI GCNGC 4 cut(s) 153, 305, 308, 421
BlpI GCTNAGC 1 cut(s) 343
BlsI GCNGC 4 cut(s) 154, 306, 309, 422
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 278
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 197
Bpu1102I GCTNAGC 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 84, 207
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 84, 207
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 357, 411
BseNI ACTGG 1 cut(s) 439
BsePI GCGCGC 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 164, 291, 319, 432
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 6, 84
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 84
BsnI GGCC 1 cut(s) 280
Bso31I GGTCTC 1 cut(s) 84
Bsp143I GATC 4 cut(s) 61, 403, 445, 450
Bsp1720I GCTNAGC 1 cut(s) 343
BspACI CCGC 3 cut(s) 295, 378, 423
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 2 cut(s) 356, 410
BspFNI CGCG 1 cut(s) 222
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 4 cut(s) 69, 411, 445, 453
BspTNI GGTCTC 1 cut(s) 84
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 84, 207
BssHII GCGCGC 1 cut(s) 220
BssMI GATC 4 cut(s) 61, 403, 445, 450
BssSI CACGAG 2 cut(s) 354, 461
BssT1I CCWWGG 1 cut(s) 207
Bst2BI CACGAG 2 cut(s) 354, 461
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 343, 397
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 3 cut(s) 222, 224, 342
BstKTI GATC 4 cut(s) 64, 406, 448, 453
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 4 cut(s) 61, 403, 445, 450
BstMWI GCNNNNNNNGC 2 cut(s) 245, 277
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 305
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 164, 291, 319, 432
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 2 cut(s) 61, 450
BstYI RGATCY 2 cut(s) 61, 450
BsuRI GGCC 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 3 cut(s) 222, 224, 342
Cfr13I GGNCC 2 cut(s) 211, 278
CviAII CATG 4 cut(s) 16, 116, 127, 215
CviJI RGCY 8 cut(s) 101, 137, 271, 280, 304, 310, 347, 438
CviKI_1 RGCY 8 cut(s) 101, 137, 271, 280, 304, 310, 347, 438
DdeI CTNAG 2 cut(s) 343, 397
DpnI GATC 4 cut(s) 63, 405, 447, 452
DpnII GATC 4 cut(s) 61, 403, 445, 450
EciI GGCGGA 2 cut(s) 284, 393
Eco130I CCWWGG 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 4 cut(s) 19, 119, 130, 218
FaiI YATR 9 cut(s) 17, 59, 117, 128, 171, 179, 216, 386, 441
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FatI CATG 4 cut(s) 15, 115, 126, 214
Fnu4HI GCNGC 4 cut(s) 153, 305, 308, 421
Fsp4HI GCNGC 4 cut(s) 153, 305, 308, 421
FspBI CTAG 2 cut(s) 65, 410
GlaI GCGC 3 cut(s) 221, 223, 341
GluI GCNGC 4 cut(s) 153, 305, 308, 421
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 1 cut(s) 280
HapII CCGG 2 cut(s) 6, 84
HhaI GCGC 3 cut(s) 222, 224, 342
Hin1II CATG 4 cut(s) 19, 119, 130, 218
Hin6I GCGC 3 cut(s) 220, 222, 340
HinP1I GCGC 3 cut(s) 220, 222, 340
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 2 cut(s) 193, 233
HpaII CCGG 2 cut(s) 6, 84
HphI GGTGA 3 cut(s) 80, 86, 380
Hpy188I TCNGA 2 cut(s) 400, 450
HpyAV CCTTC 2 cut(s) 141, 359
HpyCH4V TGCA 2 cut(s) 11, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 277
HpyF3I CTNAG 2 cut(s) 343, 397
Hsp92II CATG 4 cut(s) 19, 119, 130, 218
HspAI GCGC 3 cut(s) 220, 222, 340
Kzo9I GATC 4 cut(s) 61, 403, 445, 450
LmnI GCTCC 2 cut(s) 31, 263
LpnPI CCDG 6 cut(s) 19, 97, 128, 311, 335, 420
Lsp1109I GCAGC 4 cut(s) 164, 291, 319, 432
LweI GCATC 1 cut(s) 70
MaeI CTAG 2 cut(s) 65, 410
MaeIII GTNAC 2 cut(s) 74, 425
MalI GATC 4 cut(s) 63, 405, 447, 452
MboI GATC 4 cut(s) 61, 403, 445, 450
MboII GAAGA 1 cut(s) 202
MflI RGATCY 2 cut(s) 61, 450
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 9 cut(s) 16, 31, 139, 142, 167, 353, 394, 406, 436
MseI TTAA 2 cut(s) 27, 159
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 423
MspI CCGG 2 cut(s) 6, 84
MspR9I CCNGG 1 cut(s) 85
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 245, 277
NciI CCSGG 1 cut(s) 85
NdeII GATC 4 cut(s) 61, 403, 445, 450
NlaIII CATG 4 cut(s) 19, 119, 130, 218
NmuCI GTSAC 1 cut(s) 74
PauI GCGCGC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 4 cut(s) 154, 306, 309, 422
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 211, 278
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 2 cut(s) 61, 450
PteI GCGCGC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 2 cut(s) 27, 159
SatI GCNGC 4 cut(s) 153, 305, 308, 421
Sau3AI GATC 4 cut(s) 61, 403, 445, 450
Sau96I GGNCC 2 cut(s) 211, 278
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 85
SetI ASST 7 cut(s) 27, 42, 70, 103, 306, 349, 370
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 305
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 156
SsiI CCGC 3 cut(s) 295, 378, 423
SspMI CTAG 2 cut(s) 65, 410
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 207
TasI AATT 1 cut(s) 156
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 27, 159
Tru9I TTAA 2 cut(s) 27, 159
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 4 cut(s) 152, 304, 307, 420
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 2 cut(s) 123, 401
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 211
XspI CTAG 2 cut(s) 65, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.