MD16G1070200.v1.1

Belongs to the endosulfine family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006518
Physical Location & Seq
Forward (+)
4930940 .. 4933283
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1070200.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTCTGGTACAAACATGGAGGAGGTTAAGGACCAAGAGGTTGCTGATAGCACTCCAATGGATCTAGGTGATGGTGACAATGCCGGGGTAGACCCAAAAGCTTCAAATGAAAATCATGGAAATCCCATGCCTTCAGCCCAGCAGGAGGAGGCAGCAATTAAGAAAAAGTATGGAGGCATACTGCCAAGGAAGACTCCCCTAATATCCAAGGACCATGAGCGTGCTTATTTTGATTCTGCTGATTGGGCACTTGGAAAGCAAGGTGCAAAGCCGAAAGGGCCGCTGGAAGCACTCCGCCCAAAGCTGCAGCCCACACCGCACCAGCAAGGGCGTTCAAGGCGCTCAGCTTATTCTTCTGGAGGCGAAGGTGATGATGGCGGAAATATGAACATTTCATCGGAGGACCAACTAGATGGTGGCAGCGGTGACAACACTGGCGATGAGGATCAGACCCGCCACCACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

156

Amino Acids

16.6

Weight (kDa)

4.91

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 44 - 116 9.6e-26 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AciI CCGC 6 cut(s) 281, 295, 317, 378, 423, 454
AclWI GGATC 2 cut(s) 69, 453
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 105, 336
AluBI AGCT 3 cut(s) 101, 304, 347
AluI AGCT 3 cut(s) 101, 304, 347
AlwI GGATC 2 cut(s) 69, 453
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 4 cut(s) 152, 304, 307, 420
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 4 cut(s) 31, 211, 278, 403
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 4 cut(s) 80, 86, 380, 437
AvaII GGWCC 3 cut(s) 31, 211, 403
BaeGI GKGCMC 1 cut(s) 250
BauI CACGAG 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 197
BbvI GCAGC 4 cut(s) 164, 291, 319, 432
BccI CCATC 3 cut(s) 65, 368, 407
BcnI CCSGG 1 cut(s) 85
BfaI CTAG 2 cut(s) 65, 410
BfmI CTRYAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 277
BisI GCNGC 5 cut(s) 153, 281, 305, 308, 421
BlpI GCTNAGC 1 cut(s) 343
BlsI GCNGC 5 cut(s) 154, 282, 306, 309, 422
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 3 cut(s) 31, 211, 403
BmgT120I GGNCC 4 cut(s) 31, 211, 278, 403
BmrFI CCNGG 1 cut(s) 85
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 1 cut(s) 378
Bpu1102I GCTNAGC 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 444
BsaJI CCNNGG 3 cut(s) 84, 185, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 439
Bse8I GATNNNNATC 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 84, 185, 207
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 2 cut(s) 35, 161
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 4 cut(s) 164, 291, 319, 432
BseYI CCCAGC 1 cut(s) 138
BshFI GGCC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 145
BsnI GGCC 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 2 cut(s) 61, 445
Bsp1720I GCTNAGC 1 cut(s) 343
BspACI CCGC 6 cut(s) 281, 295, 317, 378, 423, 454
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 1 cut(s) 356
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 2 cut(s) 69, 453
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 84, 185, 207
BssMI GATC 2 cut(s) 61, 445
BssSI CACGAG 1 cut(s) 461
BssT1I CCWWGG 2 cut(s) 185, 207
Bst2BI CACGAG 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 343
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 2 cut(s) 64, 448
BstMBI GATC 2 cut(s) 61, 445
BstMWI GCNNNNNNNGC 4 cut(s) 245, 277, 316, 337
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 4 cut(s) 164, 291, 319, 432
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 4 cut(s) 31, 211, 278, 403
Csp6I GTAC 1 cut(s) 9
CviAII CATG 4 cut(s) 16, 116, 127, 215
CviJI RGCY 7 cut(s) 101, 137, 271, 280, 304, 310, 347
CviKI_1 RGCY 7 cut(s) 101, 137, 271, 280, 304, 310, 347
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 1 cut(s) 343
DpnI GATC 2 cut(s) 63, 447
DpnII GATC 2 cut(s) 61, 445
EciI GGCGGA 2 cut(s) 284, 393
Eco130I CCWWGG 2 cut(s) 185, 207
Eco47I GGWCC 3 cut(s) 31, 211, 403
Eco57I CTGAAG 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 185, 207
ErhI CCWWGG 2 cut(s) 185, 207
FaeI CATG 4 cut(s) 19, 119, 130, 218
FaiI YATR 7 cut(s) 17, 117, 128, 171, 179, 216, 386
FatI CATG 4 cut(s) 15, 115, 126, 214
FauI CCCGC 1 cut(s) 461
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 5 cut(s) 153, 281, 305, 308, 421
Fsp4HI GCNGC 5 cut(s) 153, 281, 305, 308, 421
FspBI CTAG 2 cut(s) 65, 410
GlaI GCGC 1 cut(s) 341
GluI GCNGC 5 cut(s) 153, 281, 305, 308, 421
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 378
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 1 cut(s) 280
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 342
Hin1II CATG 4 cut(s) 19, 119, 130, 218
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 2 cut(s) 193, 233
HpaII CCGG 1 cut(s) 84
HphI GGTGA 4 cut(s) 80, 86, 380, 437
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 400, 450
Hpy188III TCNNGA 1 cut(s) 357
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 141, 359
HpyCH4V TGCA 2 cut(s) 266, 307
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 277, 316, 337
HpyF3I CTNAG 1 cut(s) 343
Hsp92II CATG 4 cut(s) 19, 119, 130, 218
HspAI GCGC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 61, 445
LpnPI CCDG 7 cut(s) 97, 128, 152, 269, 335, 342, 420
Lsp1109I GCAGC 4 cut(s) 164, 291, 319, 432
MaeI CTAG 2 cut(s) 65, 410
MaeIII GTNAC 2 cut(s) 74, 425
MalI GATC 2 cut(s) 63, 447
MboI GATC 2 cut(s) 61, 445
MboII GAAGA 2 cut(s) 202, 345
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 9 cut(s) 13, 16, 31, 139, 142, 167, 353, 394, 436
MseI TTAA 2 cut(s) 27, 159
MslI CAYNNNNRTG 2 cut(s) 56, 219
MspA1I CMGCKG 2 cut(s) 283, 423
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 85
MwoI GCNNNNNNNGC 4 cut(s) 245, 277, 316, 337
NciI CCSGG 1 cut(s) 85
NdeII GATC 2 cut(s) 61, 445
NlaIII CATG 4 cut(s) 19, 119, 130, 218
NmuCI GTSAC 2 cut(s) 74, 425
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 154, 282, 306, 309, 422
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 138
PspPI GGNCC 4 cut(s) 31, 211, 278, 403
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 1 cut(s) 61
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 2 cut(s) 56, 219
SaqAI TTAA 2 cut(s) 27, 159
SatI GCNGC 5 cut(s) 153, 281, 305, 308, 421
Sau3AI GATC 2 cut(s) 61, 445
Sau96I GGNCC 4 cut(s) 31, 211, 278, 403
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 1 cut(s) 250
SetI ASST 8 cut(s) 27, 42, 70, 103, 265, 306, 349, 370
SfcI CTRYAG 1 cut(s) 305
SinI GGWCC 3 cut(s) 31, 211, 403
SmiMI CAYNNNNRTG 2 cut(s) 56, 219
Sse9I AATT 1 cut(s) 156
SsiI CCGC 6 cut(s) 281, 295, 317, 378, 423, 454
SspMI CTAG 2 cut(s) 65, 410
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 2 cut(s) 185, 207
TasI AATT 1 cut(s) 156
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 27, 159
Tru9I TTAA 2 cut(s) 27, 159
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 2 cut(s) 74, 425
TseI GCWGC 4 cut(s) 152, 304, 307, 420
Tsp45I GTSAC 2 cut(s) 74, 425
TspDTI ATGAA 3 cut(s) 123, 384, 401
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 3 cut(s) 31, 211, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 2 cut(s) 65, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.