FvH4_4g33012

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008208
Physical Location & Seq
Forward (+)
31399256 .. 31403621
4366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33012.t2

Sequence Viewer

Length: 1017 bp
ATGCAGGTTTTCTCCTTCCGACGTCAGTTCATGATCCTCACGGCCTGCTTCTCCCTTTGTTTGGTTGCTAAAGCTGAGTTTCAACGGTTTCAACTGCAAACAAACGTCGAAGACTCTCTGAAATTCTTCGTTGTTGGAGACTGGGGCAGACGAGGACTCTACAATCAATCTCAAGTTGCAGCCCAGATGGGATTAGTTGCGAACAAAGAAAACATAGACATTGTCTTCTCCACCGGTGACAATTTCTACGAAGATGGTTTGAAAGGCGTAGATGATCCGGCTTTTAAAGAATCATTCTCCAACATATACAATGCCCAAAGCTTACAGAAACCATATTGGTTCAATGTATTAGGTAACCATGACTATCGTGGTGACGTTCTAGCACAACTGAATCCAGTCCTCAAAGGAATTGACAGTAGATGGTTTTGCTATAGATCATTCCATATCGGTTATGCAGATTTCCTGGAAATTTTCTCTGTCGACACGACGCCATTCGTAGATGACTACTTCACAGATCCAAAGGAGCATGTCTATGACTGGAGAGGTGTGCTTCCTCGAGAGAATTACCTTTCAAATGTCTTGAAGGACCTGGATAATGCACTGAAAAATTCCTCTGCAAAATGGAAAATTGTGATCGGTCACCATACAATTAGAAGCGCAGGACATCATGGTAACACTGTTGAGCTTGTAACCAAACTTCTTCCAGTCCTTAAGGCAAACAATATAGATTTTTATATGAACGGACATGACCATTGCTTGCAGCACATCAGTGATCCTGACAGTAAAATTGAGTTTTTTACCAGTGGAGGTGGCTCCAAGGCATGGAGAGGTGATATAAAGCCCTGGGATCCGAAAGAATTGAAGCTGTACTACGACGGCCAAGGTTTTTTATCAGCAAAAATAAATAAAACCACTGCTGATATACAATTTTACGATGTCTCTGGCAATGTTTTGCACAATTGGAGCAAATCCAAGGACCTAGACTCAGTCACGCATAGAAAGTTTATCTATGCTTAA

Protein Analysis

339

Amino Acids

38.82

Weight (kDa)

6.45

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 40 - 252 4.5e-15 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 822
AccI GTMKAC 1 cut(s) 480
AclWI GGATC 6 cut(s) 28, 269, 509, 767, 842, 855
AcoI YGGCCR 1 cut(s) 877
AcsI RAATTY 3 cut(s) 122, 468, 607
AcyI GRCGYC 2 cut(s) 22, 488
AfaI GTAC 1 cut(s) 869
AfiI CCNNNNNNNGG 2 cut(s) 61, 822
AflII CTTAAG 1 cut(s) 710
AgeI ACCGGT 1 cut(s) 233
AgsI TTSAA 7 cut(s) 83, 92, 262, 343, 573, 583, 862
AjnI CCWGG 3 cut(s) 462, 588, 842
AleI CACNNNNGTG 1 cut(s) 768
AluBI AGCT 4 cut(s) 74, 321, 685, 865
AluI AGCT 4 cut(s) 74, 321, 685, 865
Alw26I GTCTC 2 cut(s) 132, 943
AlwI GGATC 6 cut(s) 28, 269, 509, 767, 842, 855
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 2 cut(s) 42, 877
ApeKI GCWGC 2 cut(s) 179, 760
ApoI RAATTY 3 cut(s) 122, 468, 607
AsiGI ACCGGT 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 586, 976
AsuHPI GGTGA 4 cut(s) 248, 383, 632, 842
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 2 cut(s) 586, 976
BamHI GGATCC 1 cut(s) 847
BarI GAAGNNNNNNTAC 4 cut(s) 681, 713, 854, 886
BbsI GAAGAC 2 cut(s) 117, 217
BbvI GCAGC 2 cut(s) 191, 772
BccI CCATC 3 cut(s) 181, 248, 414
BceAI ACGGC 2 cut(s) 57, 892
BciT130I CCWGG 3 cut(s) 464, 590, 844
BcoDI GTCTC 2 cut(s) 132, 943
BfaI CTAG 2 cut(s) 380, 980
BfmI CTRYAG 1 cut(s) 430
BfrI CTTAAG 1 cut(s) 710
BisI GCNGC 2 cut(s) 180, 761
BlsI GCNGC 2 cut(s) 181, 762
Bme1390I CCNGG 3 cut(s) 464, 590, 844
Bme18I GGWCC 2 cut(s) 586, 976
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 2 cut(s) 586, 976
BmiI GGNNCC 2 cut(s) 814, 849
BmrFI CCNGG 3 cut(s) 464, 590, 844
BmrI ACTGGG 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 2 cut(s) 117, 217
BpmI CTGGAG 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 156
BsaHI GRCGYC 2 cut(s) 22, 488
BsaJI CCNNGG 5 cut(s) 816, 842, 843, 880, 972
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 822
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 5 cut(s) 146, 395, 542, 704, 801
Bse3DI GCAATG 2 cut(s) 751, 952
BseBI CCWGG 3 cut(s) 464, 590, 844
BseDI CCNNGG 5 cut(s) 816, 842, 843, 880, 972
BseLI CCNNNNNNNGG 2 cut(s) 61, 822
BseMI GCAATG 2 cut(s) 751, 952
BseMII CTCAG 2 cut(s) 66, 999
BseNI ACTGG 5 cut(s) 146, 395, 542, 704, 801
BseXI GCAGC 2 cut(s) 191, 772
BshFI GGCC 2 cut(s) 44, 879
BshTI ACCGGT 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 2 cut(s) 234, 278
BslI CCNNNNNNNGG 2 cut(s) 61, 822
BsmAI GTCTC 2 cut(s) 132, 943
BsnI GGCC 2 cut(s) 44, 879
BsoBI CYCGRG 1 cut(s) 555
Bsp143I GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
BspANI GGCC 2 cut(s) 44, 879
BspCNI CTCAG 2 cut(s) 67, 998
BspHI TCATGA 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 814, 849
BspPI GGATC 6 cut(s) 28, 269, 509, 767, 842, 855
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 2 cut(s) 751, 952
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 5 cut(s) 146, 395, 542, 704, 801
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 816, 842, 843, 880, 972
BssMI GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
BssNI GRCGYC 2 cut(s) 22, 488
BssT1I CCWWGG 3 cut(s) 816, 880, 972
Bst2UI CCWGG 3 cut(s) 464, 590, 844
Bst4CI ACNGT 4 cut(s) 87, 416, 679, 782
BstACI GRCGYC 2 cut(s) 22, 488
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 46, 758
BstDEI CTNAG 2 cut(s) 75, 985
BstEII GGTNACC 2 cut(s) 353, 638
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 7 cut(s) 36, 277, 437, 517, 636, 775, 850
BstMAI GTCTC 2 cut(s) 132, 943
BstMBI GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
BstNI CCWGG 3 cut(s) 464, 590, 844
BstNSI RCATGY 1 cut(s) 530
BstPI GGTNACC 2 cut(s) 353, 638
BstSCI CCNGG 3 cut(s) 462, 588, 842
BstSFI CTRYAG 1 cut(s) 430
BstV1I GCAGC 2 cut(s) 191, 772
BstV2I GAAGAC 2 cut(s) 117, 217
BstX2I RGATCY 2 cut(s) 514, 847
BstYI RGATCY 2 cut(s) 514, 847
BsuRI GGCC 2 cut(s) 44, 879
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 5 cut(s) 599, 675, 775, 808, 912
Cac8I GCNNGC 2 cut(s) 46, 758
CciI TCATGA 1 cut(s) 30
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 586, 976
CseI GACGC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 868
CspAI ACCGGT 1 cut(s) 233
CviAII CATG 6 cut(s) 31, 359, 527, 668, 746, 822
CviQI GTAC 1 cut(s) 868
DdeI CTNAG 2 cut(s) 75, 985
DpnI GATC 7 cut(s) 35, 276, 436, 516, 635, 774, 849
DpnII GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 877
Eco130I CCWWGG 3 cut(s) 816, 880, 972
Eco47I GGWCC 2 cut(s) 586, 976
Eco88I CYCGRG 1 cut(s) 555
Eco91I GGTNACC 2 cut(s) 353, 638
EcoO109I RGGNCCY 2 cut(s) 586, 976
EcoO65I GGTNACC 2 cut(s) 353, 638
EcoRII CCWGG 3 cut(s) 462, 588, 842
EcoT14I CCWWGG 3 cut(s) 816, 880, 972
ErhI CCWWGG 3 cut(s) 816, 880, 972
FaeI CATG 6 cut(s) 34, 362, 530, 671, 749, 825
FatI CATG 6 cut(s) 30, 358, 526, 667, 745, 821
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 2 cut(s) 180, 761
Fsp4HI GCNGC 2 cut(s) 180, 761
FspBI CTAG 2 cut(s) 380, 980
GlaI GCGC 1 cut(s) 658
GluI GCNGC 2 cut(s) 180, 761
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 2 cut(s) 44, 879
HapII CCGG 2 cut(s) 234, 278
HgaI GACGC 1 cut(s) 496
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 2 cut(s) 22, 488
Hin1II CATG 6 cut(s) 34, 362, 530, 671, 749, 825
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 5 cut(s) 113, 156, 290, 391, 983
HpaII CCGG 2 cut(s) 234, 278
HphI GGTGA 4 cut(s) 248, 383, 632, 842
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 3 cut(s) 20, 120, 852
Hpy188III TCNNGA 4 cut(s) 31, 557, 580, 776
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 4 cut(s) 24, 110, 490, 878
HpyAV CCTTC 2 cut(s) 25, 577
HpyCH4III ACNGT 4 cut(s) 87, 416, 679, 782
HpyCH4IV ACGT 3 cut(s) 22, 105, 375
HpyCH4V TGCA 8 cut(s) 4, 97, 179, 455, 599, 617, 760, 955
HpyF3I CTNAG 2 cut(s) 75, 985
HpySE526I ACGT 3 cut(s) 22, 105, 375
Hsp92I GRCGYC 2 cut(s) 22, 488
Hsp92II CATG 6 cut(s) 34, 362, 530, 671, 749, 825
HspAI GCGC 1 cut(s) 657
Kzo9I GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
LmnI GCTCC 3 cut(s) 523, 818, 963
Lsp1109I GCAGC 2 cut(s) 191, 772
MaeI CTAG 2 cut(s) 380, 980
MaeII ACGT 3 cut(s) 22, 105, 375
MaeIII GTNAC 7 cut(s) 236, 353, 371, 638, 671, 688, 988
MalI GATC 7 cut(s) 35, 276, 436, 516, 635, 774, 849
MboI GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
MboII GAAGA 5 cut(s) 118, 122, 217, 263, 692
MfeI CAATTG 1 cut(s) 958
MflI RGATCY 2 cut(s) 514, 847
MlyI GAGTC 3 cut(s) 107, 150, 977
MmeI TCCRAC 3 cut(s) 43, 115, 324
MnlI CCTC 8 cut(s) 47, 146, 410, 536, 564, 622, 800, 821
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 285, 711, 1015
MslI CAYNNNNRTG 2 cut(s) 531, 768
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 2 cut(s) 234, 278
MspR9I CCNGG 3 cut(s) 464, 590, 844
MunI CAATTG 1 cut(s) 958
MvaI CCWGG 3 cut(s) 464, 590, 844
NdeII GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
NlaIII CATG 6 cut(s) 34, 362, 530, 671, 749, 825
NlaIV GGNNCC 2 cut(s) 814, 849
NmuCI GTSAC 4 cut(s) 236, 371, 638, 988
NspI RCATGY 1 cut(s) 530
OliI CACNNNNGTG 1 cut(s) 768
PaeR7I CTCGAG 1 cut(s) 555
PagI TCATGA 1 cut(s) 30
PasI CCCWGGG 1 cut(s) 843
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 290, 391
PflFI GACNNNGTC 2 cut(s) 221, 986
PflMI CCANNNNNTGG 1 cut(s) 822
PfoI TCCNGGA 1 cut(s) 462
PinAI ACCGGT 1 cut(s) 233
PkrI GCNGC 2 cut(s) 181, 762
PleI GAGTC 3 cut(s) 107, 150, 977
PpsI GAGTC 3 cut(s) 107, 150, 977
PpuMI RGGWCCY 2 cut(s) 586, 976
Psp5II RGGWCCY 2 cut(s) 586, 976
Psp6I CCWGG 3 cut(s) 462, 588, 842
PspEI GGTNACC 2 cut(s) 353, 638
PspGI CCWGG 3 cut(s) 462, 588, 842
PspN4I GGNNCC 2 cut(s) 814, 849
PspPI GGNCC 2 cut(s) 586, 976
PspPPI RGGWCCY 2 cut(s) 586, 976
PsuI RGATCY 2 cut(s) 514, 847
PsyI GACNNNGTC 2 cut(s) 221, 986
RsaI GTAC 1 cut(s) 869
RsaNI GTAC 1 cut(s) 868
RseI CAYNNNNRTG 2 cut(s) 531, 768
SalI GTCGAC 1 cut(s) 479
SaqAI TTAA 3 cut(s) 285, 711, 1015
SatI GCNGC 2 cut(s) 180, 761
Sau3AI GATC 7 cut(s) 33, 274, 434, 514, 633, 772, 847
Sau96I GGNCC 2 cut(s) 586, 976
SchI GAGTC 3 cut(s) 107, 150, 977
ScrFI CCNGG 3 cut(s) 464, 590, 844
SfcI CTRYAG 1 cut(s) 430
Sfr274I CTCGAG 1 cut(s) 555
SgrAI CRCCGGYG 1 cut(s) 233
SinI GGWCC 2 cut(s) 586, 976
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 2 cut(s) 531, 768
SmlI CTYRAG 3 cut(s) 171, 555, 710
SmoI CTYRAG 3 cut(s) 171, 555, 710
SspMI CTAG 2 cut(s) 380, 980
StyD4I CCNGG 3 cut(s) 462, 588, 842
StyI CCWWGG 3 cut(s) 816, 880, 972
TaaI ACNGT 4 cut(s) 87, 416, 679, 782
TaiI ACGT 3 cut(s) 25, 108, 378
TaqI TCGA 3 cut(s) 108, 480, 556
TaqII GACCGA 1 cut(s) 626
TatI WGTACW 1 cut(s) 867
TfiI GAWTC 2 cut(s) 290, 391
Tru1I TTAA 3 cut(s) 285, 711, 1015
Tru9I TTAA 3 cut(s) 285, 711, 1015
TscAI CASTG 5 cut(s) 606, 682, 775, 808, 919
TseFI GTSAC 4 cut(s) 236, 371, 638, 988
TseI GCWGC 2 cut(s) 179, 760
Tsp45I GTSAC 4 cut(s) 236, 371, 638, 988
TspDTI ATGAA 2 cut(s) 19, 752
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 5 cut(s) 606, 682, 775, 808, 919
Tth111I GACNNNGTC 2 cut(s) 221, 986
Van91I CCANNNNNTGG 1 cut(s) 822
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 586, 976
XapI RAATTY 3 cut(s) 122, 468, 607
XceI RCATGY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XhoI CTCGAG 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 380, 980
ZraI GACGTC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.