Rh4CG418700

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008208
Physical Location & Seq
Forward (+)
66339672 .. 66340755
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG418700.1

Sequence Viewer

Length: 705 bp
ATGCAGCGTTTCTCATTTCCTCAATTCTTGATCCTCACGGCTTGCCTCTCACTTTGTTTGGTTGCTAAAGCTGATCTTCAACCTTTTCAACAGCAAGCAAATGCAGATGGGTCTTTGAGTTTCTTGGTCGTTGGAGATTGGGGAAGGCGAGGACTCTACAACCAATCTAAAGTCGCTACCCAGATGGGAATAATTGGAGAGAAAGCACAGACAAACTTCGTCATCTCCACCGGCGACAATTTCTACGATGATGGCTTGACAGGGCCGGATGATCCAGCCTTCAATGAGTCATTTTCCAACATATACACTGCCCCAAGCTTAAAGATACCTTGGTACATTGTGTTGGGCAACCATGACTATAGGGGTGATGCTTTAGCACAACTAAAAATTGACCGTAGATGGTTTTGCTCCAGGCCATTGTTCATTGTATATTCAGCAATCGCAGAGTTTTTCTTCGTGGACACAACCCCATTTGTGGATGACTACTTTACAAATCCAAAGGAGCATGTCTATGACTGGAGAGGTGTGCTTCCTCGAGAGGATTACCTTTCAAAACTCCTGAAGGATCTGGATGTAACACTGAAAAATTCCCGTGCAAAATGGAAAATTGTGGTCGGTCACCATACAATTAGAAGTGCAGGACATCATGGTAACACCACTGATCTTGTAACCCACCTTCTCCCAGTCCTTAAGGTAATCCCTTAA

Protein Analysis

234

Amino Acids

26.52

Weight (kDa)

6.64

Isoelectric Point (pI)

25.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 40 - 221 1.5e-14 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 25, 266, 573
AcsI RAATTY 1 cut(s) 586
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 475
AflII CTTAAG 1 cut(s) 689
AgsI TTSAA 4 cut(s) 80, 89, 283, 552
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 2 cut(s) 71, 318
AluI AGCT 2 cut(s) 71, 318
AlwI GGATC 3 cut(s) 25, 266, 573
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 2 cut(s) 263, 413
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 377, 611
AvaI CYCGRG 1 cut(s) 534
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BbvI GCAGC 1 cut(s) 16
BccI CCATC 4 cut(s) 101, 178, 245, 393
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 412
BfmI CTRYAG 1 cut(s) 358
BfrI CTTAAG 1 cut(s) 689
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 412
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 677
BpmI CTGGAG 2 cut(s) 394, 538
BsaJI CCNNGG 1 cut(s) 329
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 521, 683
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 3 cut(s) 274, 484, 577
BseLI CCNNNNNNNGG 1 cut(s) 475
BseNI ACTGG 2 cut(s) 521, 683
BseXI GCAGC 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 2 cut(s) 265, 415
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 2 cut(s) 231, 266
BslI CCNNNNNNNGG 1 cut(s) 475
BsnI GGCC 2 cut(s) 265, 415
BsoBI CYCGRG 1 cut(s) 534
Bsp143I GATC 5 cut(s) 30, 73, 271, 565, 661
BspANI GGCC 2 cut(s) 265, 415
BspPI GGATC 3 cut(s) 25, 266, 573
BspTI CTTAAG 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 2 cut(s) 521, 683
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 5 cut(s) 30, 73, 271, 565, 661
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 395
BstAFI CTTAAG 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 43, 96
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 3 cut(s) 274, 484, 577
BstKTI GATC 5 cut(s) 33, 76, 274, 568, 664
BstMBI GATC 5 cut(s) 30, 73, 271, 565, 661
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 2 cut(s) 265, 415
BtsCI GGATG 3 cut(s) 274, 484, 577
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 306, 578, 657
Cac8I GCNNGC 2 cut(s) 43, 96
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 334
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 3 cut(s) 353, 506, 647
CviJI RGCY 7 cut(s) 41, 71, 255, 265, 278, 318, 415
CviKI_1 RGCY 7 cut(s) 41, 71, 255, 265, 278, 318, 415
CviQI GTAC 1 cut(s) 334
DpnI GATC 5 cut(s) 32, 75, 273, 567, 663
DpnII GATC 5 cut(s) 30, 73, 271, 565, 661
Eco130I CCWWGG 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 581
Eco88I CYCGRG 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 617
EcoO65I GGTNACC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 3 cut(s) 356, 509, 650
FaiI YATR 9 cut(s) 302, 304, 354, 360, 430, 507, 513, 624, 648
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 3 cut(s) 352, 505, 646
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 281, 491, 584
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 2 cut(s) 394, 538
HaeIII GGCC 2 cut(s) 265, 415
HapII CCGG 2 cut(s) 231, 266
Hin1II CATG 3 cut(s) 356, 509, 650
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 2 cut(s) 153, 287
HpaII CCGG 2 cut(s) 231, 266
HphI GGTGA 2 cut(s) 377, 611
Hpy166II GTNNAC 1 cut(s) 460
Hpy188III TCNNGA 4 cut(s) 28, 536, 559, 569
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 4 cut(s) 138, 289, 556, 686
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4V TGCA 4 cut(s) 4, 104, 596, 638
Hsp92II CATG 3 cut(s) 356, 509, 650
Kzo9I GATC 5 cut(s) 30, 73, 271, 565, 661
LmnI GCTCC 2 cut(s) 413, 502
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 358
MaeIII GTNAC 4 cut(s) 574, 617, 650, 667
MalI GATC 5 cut(s) 32, 75, 273, 567, 663
MboI GATC 5 cut(s) 30, 73, 271, 565, 661
MboII GAAGA 2 cut(s) 68, 445
MflI RGATCY 1 cut(s) 565
MluCI AATT 7 cut(s) 23, 192, 238, 387, 586, 606, 627
MlyI GAGTC 2 cut(s) 147, 296
MmeI TCCRAC 2 cut(s) 112, 321
MnlI CCTC 7 cut(s) 30, 44, 56, 143, 515, 532, 543
MseI TTAA 3 cut(s) 320, 690, 703
MslI CAYNNNNRTG 1 cut(s) 510
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 2 cut(s) 231, 266
MspR9I CCNGG 1 cut(s) 412
MvaI CCWGG 1 cut(s) 412
NdeII GATC 5 cut(s) 30, 73, 271, 565, 661
NlaIII CATG 3 cut(s) 356, 509, 650
NmuCI GTSAC 1 cut(s) 617
NspI RCATGY 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 534
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 2 cut(s) 147, 295
PpsI GAGTC 2 cut(s) 147, 295
Psp6I CCWGG 1 cut(s) 410
PspEI GGTNACC 1 cut(s) 617
PspGI CCWGG 1 cut(s) 410
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 3 cut(s) 320, 690, 703
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 5 cut(s) 30, 73, 271, 565, 661
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 147, 296
ScrFI CCNGG 1 cut(s) 412
SetI ASST 8 cut(s) 73, 85, 320, 331, 526, 549, 678, 696
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 534
SgrAI CRCCGGYG 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 534
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 2 cut(s) 534, 689
SmoI CTYRAG 2 cut(s) 534, 689
Sse9I AATT 7 cut(s) 23, 192, 238, 387, 586, 606, 627
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 535
TaqII GACCGA 1 cut(s) 605
TasI AATT 7 cut(s) 23, 192, 238, 387, 586, 606, 627
Tru1I TTAA 3 cut(s) 320, 690, 703
Tru9I TTAA 3 cut(s) 320, 690, 703
TscAI CASTG 3 cut(s) 313, 585, 664
TseFI GTSAC 1 cut(s) 617
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 617
TspDTI ATGAA 1 cut(s) 412
TspRI CASTG 3 cut(s) 313, 585, 664
Vha464I CTTAAG 1 cut(s) 689
XapI RAATTY 1 cut(s) 586
XceI RCATGY 1 cut(s) 509
XhoI CTCGAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.