Rh4BG403500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008208
Physical Location & Seq
Forward (+)
55428007 .. 55430001
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG403500.1

Sequence Viewer

Length: 1053 bp
ATGGCTTCTTTGTACTCTTCGAATTCCAACAAAACCATGCAGCTTTTATCCTTCCGACGTCAGTTCATGATCCTCACGGCTTGTCTCTCACTTTGTTTGGTTGCTAAAGCTGAGTTTCAACGTTTGCAACAGCAAACAAACGACGAAGGGTCTCTGAAATTCTTCGTTGTTGGAGATTGGGGCAGACGAGGACTCTACAACCAATCTCAAGTTGCAGCCCAGATGGGATTAATCGCGAACAAAGAAAACATAGACATTGTTTTCTCCACCGGTGACAATTTCTACGACGATGGTTTGAAAGGCGTTTATGATCCAGCTTTTAAAGAATCATTCTCCAACATCTACAATGCCCAAAGCTTAAAGAAACCATATTGGTTCAGTGTATTGGGTAACCATGACTATCGTGGTAACGTTCTAGCACAACTGAATCCACTCCTCAAACGCATTGACAGTAGATGGTTTTGCTATAGATCATTCCATATCGGTTATGCAGATTTCGTAGAGATTTTCTCTGTGGACACAACCCCATTTGTAGATGAGTACTTCACGGATCCAAAGGAGCATGTCTATGACTGGAGAGGTGTGCTTCCTCGGGAGGATTACCTTTCAAATGTCTTGAAGGGTCTGGATCATGCACTGGAAAATTCCTCTGCAAAATGGAAAATTGTGATCGGTCACCATACAATTAGAAGTGCAGGACATCATGGTAACACCGCTGAGCTTGTAACCAAACTTCTCCCAATCCGTAAGGCAAACAATATAGATTTTTATATAAACGGACATGACCATTGCTTGCAGCACATCAGTGATACTGACAGTAAAATTGAGTTTTTTACCAGTGGAGGTGGCTCCAAGGCATGGAGAGGTGATATAGATCCCTGGGATCCAAGAGAATTGAAGCTGTACTACGACGGCCAAGGTTTTTTGTCGGCCAAAATAACTAAAACCAACGCTGATATACAATTTTACGATGTCTCTGGCAATGTTTTGCACAATTGGAGCAAATCCAAGGTCCTAGACTCGGTCACGCATAGAAAGTTTATCTATGCTTAA

Protein Analysis

350

Amino Acids

40.11

Weight (kDa)

7.22

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 52 - 264 2.6e-16 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 61
AccB7I CCANNNNNTGG 1 cut(s) 858
AccII CGCG 1 cut(s) 236
AciI CCGC 1 cut(s) 714
AclI AACGTT 2 cut(s) 121, 411
AclWI GGATC 8 cut(s) 64, 305, 545, 558, 636, 869, 878, 891
AcoI YGGCCR 2 cut(s) 913, 930
AcsI RAATTY 3 cut(s) 22, 158, 643
AcyI GRCGYC 1 cut(s) 58
AfaI GTAC 3 cut(s) 14, 542, 905
AfiI CCNNNNNNNGG 2 cut(s) 858, 1021
AgeI ACCGGT 1 cut(s) 269
AgsI TTSAA 5 cut(s) 119, 298, 609, 619, 898
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 878
AleI CACNNNNGTG 1 cut(s) 804
AluBI AGCT 6 cut(s) 43, 110, 317, 357, 721, 901
AluI AGCT 6 cut(s) 43, 110, 317, 357, 721, 901
Alw26I GTCTC 3 cut(s) 89, 156, 979
AlwI GGATC 8 cut(s) 64, 305, 545, 558, 636, 869, 878, 891
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 2 cut(s) 913, 930
ApeKI GCWGC 3 cut(s) 40, 215, 796
ApoI RAATTY 3 cut(s) 22, 158, 643
ArsI GACNNNNNNTTYG 2 cut(s) 996, 1028
AseI ATTAAT 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 1012
AsuHPI GGTGA 3 cut(s) 284, 668, 878
AsuII TTCGAA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 1 cut(s) 1012
BamHI GGATCC 2 cut(s) 550, 883
BarI GAAGNNNNNNTAC 4 cut(s) 717, 749, 890, 922
BbvI GCAGC 3 cut(s) 52, 227, 808
BccI CCATC 3 cut(s) 217, 284, 450
BceAI ACGGC 2 cut(s) 93, 928
BciT130I CCWGG 1 cut(s) 880
BcoDI GTCTC 3 cut(s) 89, 156, 979
BfaI CTAG 2 cut(s) 416, 1016
BfmI CTRYAG 1 cut(s) 466
BisI GCNGC 3 cut(s) 41, 216, 797
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 3 cut(s) 42, 217, 798
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 1 cut(s) 880
Bme18I GGWCC 1 cut(s) 1012
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 1012
BmiI GGNNCC 3 cut(s) 552, 850, 885
BmrFI CCNGG 1 cut(s) 880
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 595
Bpu1102I GCTNAGC 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 873
BsaHI GRCGYC 1 cut(s) 58
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 6 cut(s) 590, 852, 878, 879, 916, 1008
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 2 cut(s) 858, 1021
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 3 cut(s) 578, 642, 837
Bse3DI GCAATG 2 cut(s) 787, 988
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 6 cut(s) 590, 852, 878, 879, 916, 1008
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 2 cut(s) 858, 1021
BseMI GCAATG 2 cut(s) 787, 988
BseMII CTCAG 2 cut(s) 102, 708
BseNI ACTGG 3 cut(s) 578, 642, 837
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 52, 227, 808
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 915, 932
BshTI ACCGGT 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 1 cut(s) 270
BslI CCNNNNNNNGG 2 cut(s) 858, 1021
BsmAI GTCTC 3 cut(s) 89, 156, 979
BsnI GGCC 2 cut(s) 915, 932
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 591
Bsp119I TTCGAA 1 cut(s) 20
Bsp143I GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
Bsp1720I GCTNAGC 1 cut(s) 717
Bsp68I TCGCGA 1 cut(s) 236
BspACI CCGC 1 cut(s) 714
BspANI GGCC 2 cut(s) 915, 932
BspCNI CTCAG 2 cut(s) 103, 709
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 552, 850, 885
BspPI GGATC 8 cut(s) 64, 305, 545, 558, 636, 869, 878, 891
BspT104I TTCGAA 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 787, 988
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 3 cut(s) 578, 642, 837
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 590, 852, 878, 879, 916, 1008
BssMI GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
BssNI GRCGYC 1 cut(s) 58
BssT1I CCWWGG 3 cut(s) 852, 916, 1008
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 2 cut(s) 452, 818
Bst6I CTCTTC 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 58
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 794
BstDEI CTNAG 2 cut(s) 111, 717
BstEII GGTNACC 2 cut(s) 389, 674
BstFNI CGCG 1 cut(s) 236
BstKTI GATC 8 cut(s) 72, 313, 473, 553, 631, 672, 877, 886
BstMAI GTCTC 3 cut(s) 89, 156, 979
BstMBI GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
BstNI CCWGG 1 cut(s) 880
BstNSI RCATGY 1 cut(s) 566
BstPI GGTNACC 2 cut(s) 389, 674
BstSCI CCNGG 1 cut(s) 878
BstSFI CTRYAG 1 cut(s) 466
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 3 cut(s) 52, 227, 808
BstX2I RGATCY 3 cut(s) 550, 874, 883
BstYI RGATCY 3 cut(s) 550, 874, 883
BsuRI GGCC 2 cut(s) 915, 932
BtsIMutI CAGTG 4 cut(s) 385, 635, 811, 844
BtuMI TCGCGA 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 794
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 1012
Csp6I GTAC 3 cut(s) 13, 541, 904
CspAI ACCGGT 1 cut(s) 269
CviAII CATG 8 cut(s) 37, 67, 395, 563, 632, 704, 782, 858
CviQI GTAC 3 cut(s) 13, 541, 904
DdeI CTNAG 2 cut(s) 111, 717
DpnI GATC 8 cut(s) 71, 312, 472, 552, 630, 671, 876, 885
DpnII GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
DraI TTTAAA 1 cut(s) 322
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 913, 930
Eam1104I CTCTTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 852, 916, 1008
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 1012
Eco88I CYCGRG 1 cut(s) 591
Eco91I GGTNACC 2 cut(s) 389, 674
EcoO109I RGGNCCY 1 cut(s) 1012
EcoO65I GGTNACC 2 cut(s) 389, 674
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 878
EcoT14I CCWWGG 3 cut(s) 852, 916, 1008
ErhI CCWWGG 3 cut(s) 852, 916, 1008
FaeI CATG 8 cut(s) 40, 70, 398, 566, 635, 707, 785, 861
FatI CATG 8 cut(s) 36, 66, 394, 562, 631, 703, 781, 857
Fnu4HI GCNGC 3 cut(s) 41, 216, 797
Fsp4HI GCNGC 3 cut(s) 41, 216, 797
FspBI CTAG 2 cut(s) 416, 1016
GluI GCNGC 3 cut(s) 41, 216, 797
GsuI CTGGAG 1 cut(s) 595
HaeIII GGCC 2 cut(s) 915, 932
HapII CCGG 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 8 cut(s) 40, 70, 398, 566, 635, 707, 785, 861
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 4 cut(s) 192, 326, 427, 1019
HpaII CCGG 1 cut(s) 270
HphI GGTGA 3 cut(s) 284, 668, 878
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 56, 156
Hpy188III TCNNGA 5 cut(s) 67, 235, 593, 616, 626
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 4 cut(s) 60, 146, 290, 914
HpyAV CCTTC 3 cut(s) 61, 140, 613
HpyCH4III ACNGT 2 cut(s) 452, 818
HpyCH4IV ACGT 3 cut(s) 58, 121, 411
HpyCH4V TGCA 9 cut(s) 40, 127, 215, 491, 635, 653, 695, 796, 991
HpyF3I CTNAG 2 cut(s) 111, 717
HpySE526I ACGT 3 cut(s) 58, 121, 411
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 8 cut(s) 40, 70, 398, 566, 635, 707, 785, 861
Kzo9I GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
LmnI GCTCC 3 cut(s) 559, 854, 999
Lsp1109I GCAGC 3 cut(s) 52, 227, 808
MaeI CTAG 2 cut(s) 416, 1016
MaeII ACGT 3 cut(s) 58, 121, 411
MaeIII GTNAC 7 cut(s) 272, 389, 407, 674, 707, 724, 1024
MalI GATC 8 cut(s) 71, 312, 472, 552, 630, 671, 876, 885
MboI GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
MboII GAAGA 2 cut(s) 9, 154
MfeI CAATTG 1 cut(s) 994
MflI RGATCY 3 cut(s) 550, 874, 883
MlyI GAGTC 2 cut(s) 186, 1013
MmeI TCCRAC 4 cut(s) 51, 79, 151, 360
MnlI CCTC 9 cut(s) 83, 182, 446, 572, 589, 600, 658, 836, 857
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 4 cut(s) 230, 321, 359, 1051
MslI CAYNNNNRTG 2 cut(s) 567, 804
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 880
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 1 cut(s) 880
MvnI CGCG 1 cut(s) 236
NdeII GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
NlaIII CATG 8 cut(s) 40, 70, 398, 566, 635, 707, 785, 861
NlaIV GGNNCC 3 cut(s) 552, 850, 885
NmuCI GTSAC 3 cut(s) 272, 674, 1024
NruI TCGCGA 1 cut(s) 236
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 20
OliI CACNNNNGTG 1 cut(s) 804
PagI TCATGA 1 cut(s) 66
PasI CCCWGGG 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 326, 427
PflFI GACNNNGTC 1 cut(s) 1022
PflMI CCANNNNNTGG 1 cut(s) 858
PinAI ACCGGT 1 cut(s) 269
PkrI GCNGC 3 cut(s) 42, 217, 798
PleI GAGTC 2 cut(s) 186, 1013
PpsI GAGTC 2 cut(s) 186, 1013
PpuMI RGGWCCY 1 cut(s) 1012
PshBI ATTAAT 1 cut(s) 230
Psp1406I AACGTT 2 cut(s) 121, 411
Psp5II RGGWCCY 1 cut(s) 1012
Psp6I CCWGG 1 cut(s) 878
PspEI GGTNACC 2 cut(s) 389, 674
PspGI CCWGG 1 cut(s) 878
PspN4I GGNNCC 3 cut(s) 552, 850, 885
PspPI GGNCC 1 cut(s) 1012
PspPPI RGGWCCY 1 cut(s) 1012
PsuI RGATCY 3 cut(s) 550, 874, 883
PsyI GACNNNGTC 1 cut(s) 1022
RruI TCGCGA 1 cut(s) 236
RsaI GTAC 3 cut(s) 14, 542, 905
RsaNI GTAC 3 cut(s) 13, 541, 904
RseI CAYNNNNRTG 2 cut(s) 567, 804
SaqAI TTAA 4 cut(s) 230, 321, 359, 1051
SatI GCNGC 3 cut(s) 41, 216, 797
Sau3AI GATC 8 cut(s) 69, 310, 470, 550, 628, 669, 874, 883
Sau96I GGNCC 1 cut(s) 1012
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 2 cut(s) 186, 1013
ScrFI CCNGG 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 466
SfuI TTCGAA 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 1 cut(s) 1012
SmiMI CAYNNNNRTG 2 cut(s) 567, 804
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
SsiI CCGC 1 cut(s) 714
SspMI CTAG 2 cut(s) 416, 1016
StyD4I CCNGG 1 cut(s) 878
StyI CCWWGG 3 cut(s) 852, 916, 1008
TaaI ACNGT 2 cut(s) 452, 818
TaiI ACGT 3 cut(s) 61, 124, 414
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 2 cut(s) 662, 1012
TatI WGTACW 3 cut(s) 12, 540, 903
TfiI GAWTC 2 cut(s) 326, 427
Tru1I TTAA 4 cut(s) 230, 321, 359, 1051
Tru9I TTAA 4 cut(s) 230, 321, 359, 1051
TscAI CASTG 4 cut(s) 385, 642, 811, 844
TseFI GTSAC 3 cut(s) 272, 674, 1024
TseI GCWGC 3 cut(s) 40, 215, 796
Tsp45I GTSAC 3 cut(s) 272, 674, 1024
TspDTI ATGAA 1 cut(s) 55
TspGWI ACGGA 3 cut(s) 563, 734, 792
TspRI CASTG 4 cut(s) 385, 642, 811, 844
Tth111I GACNNNGTC 1 cut(s) 1022
Van91I CCANNNNNTGG 1 cut(s) 858
VpaK11BI GGWCC 1 cut(s) 1012
VspI ATTAAT 1 cut(s) 230
XapI RAATTY 3 cut(s) 22, 158, 643
XceI RCATGY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 416, 1016
ZraI GACGTC 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.