Rh4BG403600

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008208
Physical Location & Seq
Forward (+)
55438156 .. 55439726
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG403600.1

Sequence Viewer

Length: 819 bp
ATGCAGCGTTTCTCATTTCCTCAATTCTTGATCCTCACGGCTTGCCTCTCACTTTGTTTGGTTGCTAAAGCTGATCTTCAACCTTTTCAACAGCAAGCAAATGCAGATGGGTCTTTGAGTTTCTTGGTCGTTGGAGATTGGGGAAGGCGAGGACTCTACAACCAATCTAAAGTCGCTACCCAGATGGGAATAATTGGAGAGAAAGCACAGACAAACTTCGTCATCTCCACCGGCGACAATTTCTACGATGATGGCTTGACAGGGCCGGATGATCCAGCCTTCAATGAGTCATTTTCCAACATATACACTGCCCCAAGCTTAAAGATACCTTGGTACATTGTGTTGGGCAACCATGACTATAGGGGTGATGCTTTAGCACAACTAAAAATTGACCGTAGATGGTTTTGCTCCAGGCCATTGTTCATTGTATATTCAGCAATCGCAGAGTTTTTCTTCGTGGACACAACCCCATTTGTGGATGACTACTTTACAAATCCAAAGGAGCATGTCTATGACTGGAGAGGTGTGCTTCCTCGAGAGGATTACCTTTCAAAACTCCTGAAGCACATCAGTGATCCTGACAGTAAAATTGAGTTTTTTACCAGTGGAGGTGGCTCCAAGGCATGGAGAGGTGATATCAAGTGGTGGGATCCAAAAGAATTGAAGCTGTACTATGACGGCCAAGGTTTTATGTCGGTACAAATGACTAAAACCCGTGCTGACTTACAATTCTATGATGTCTTTGGCAATGTTTTGCACAAATGGAGCAAAACCAAAGAGCTCAACTCAGTCAGGCATAGGAAAGCTCTGATTGTATGA

Protein Analysis

272

Amino Acids

31.26

Weight (kDa)

6.96

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 40 - 222 1.8e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 624
AclWI GGATC 5 cut(s) 25, 266, 569, 644, 657
AcoI YGGCCR 1 cut(s) 679
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 3 cut(s) 335, 671, 699
AfiI CCNNNNNNNGG 2 cut(s) 475, 624
AgsI TTSAA 5 cut(s) 80, 89, 283, 552, 664
AjnI CCWGG 1 cut(s) 410
AleI CACNNNNGTG 1 cut(s) 570
AluBI AGCT 5 cut(s) 71, 318, 667, 781, 806
AluI AGCT 5 cut(s) 71, 318, 667, 781, 806
Alw21I GWGCWC 1 cut(s) 783
AlwI GGATC 5 cut(s) 25, 266, 569, 644, 657
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 3 cut(s) 263, 413, 679
ApeKI GCWGC 1 cut(s) 4
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 377, 644
AvaI CYCGRG 1 cut(s) 534
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BamHI GGATCC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 1 cut(s) 16
BccI CCATC 4 cut(s) 101, 178, 245, 393
BceAI ACGGC 2 cut(s) 54, 694
BciT130I CCWGG 1 cut(s) 412
BfmI CTRYAG 1 cut(s) 358
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 616, 651
BmrFI CCNGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 358
BplI GAGNNNNNCTC 2 cut(s) 770, 802
BpmI CTGGAG 2 cut(s) 394, 538
BsaJI CCNNGG 3 cut(s) 329, 618, 682
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 624
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 521, 603
Bse3DI GCAATG 1 cut(s) 754
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 329, 618, 682
BseGI GGATG 2 cut(s) 274, 484
BseLI CCNNNNNNNGG 2 cut(s) 475, 624
BseMI GCAATG 1 cut(s) 754
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 2 cut(s) 521, 603
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 3 cut(s) 265, 415, 681
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 2 cut(s) 231, 266
BslI CCNNNNNNNGG 2 cut(s) 475, 624
BsnI GGCC 3 cut(s) 265, 415, 681
BsoBI CYCGRG 1 cut(s) 534
Bsp1286I GDGCHC 1 cut(s) 783
Bsp143I GATC 5 cut(s) 30, 73, 271, 574, 649
BspANI GGCC 3 cut(s) 265, 415, 681
BspCNI CTCAG 1 cut(s) 800
BspLI GGNNCC 2 cut(s) 616, 651
BspPI GGATC 5 cut(s) 25, 266, 569, 644, 657
BsrDI GCAATG 1 cut(s) 754
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 2 cut(s) 521, 603
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 329, 618, 682
BssMI GATC 5 cut(s) 30, 73, 271, 574, 649
BssT1I CCWWGG 3 cut(s) 329, 618, 682
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 395, 584
BstC8I GCNNGC 2 cut(s) 43, 96
BstDEI CTNAG 1 cut(s) 787
BstF5I GGATG 2 cut(s) 274, 484
BstKTI GATC 5 cut(s) 33, 76, 274, 577, 652
BstMBI GATC 5 cut(s) 30, 73, 271, 574, 649
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 3 cut(s) 265, 415, 681
BtsCI GGATG 2 cut(s) 274, 484
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 306, 577, 610
Cac8I GCNNGC 2 cut(s) 43, 96
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 3 cut(s) 334, 670, 698
CviAII CATG 3 cut(s) 353, 506, 624
CviQI GTAC 3 cut(s) 334, 670, 698
DdeI CTNAG 1 cut(s) 787
DpnI GATC 5 cut(s) 32, 75, 273, 576, 651
DpnII GATC 5 cut(s) 30, 73, 271, 574, 649
EaeI YGGCCR 1 cut(s) 679
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 3 cut(s) 329, 618, 682
Eco24I GRGCYC 1 cut(s) 783
Eco32I GATATC 1 cut(s) 637
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 581
Eco88I CYCGRG 1 cut(s) 534
EcoICRI GAGCTC 1 cut(s) 781
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 3 cut(s) 329, 618, 682
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 3 cut(s) 329, 618, 682
FaeI CATG 3 cut(s) 356, 509, 627
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 3 cut(s) 352, 505, 623
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 281, 491
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 2 cut(s) 394, 538
HaeIII GGCC 3 cut(s) 265, 415, 681
HapII CCGG 2 cut(s) 231, 266
Hin1II CATG 3 cut(s) 356, 509, 627
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 2 cut(s) 153, 287
HpaII CCGG 2 cut(s) 231, 266
HphI GGTGA 2 cut(s) 377, 644
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 1 cut(s) 810
Hpy188III TCNNGA 4 cut(s) 28, 536, 559, 578
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 2 cut(s) 138, 289
HpyCH4III ACNGT 2 cut(s) 395, 584
HpyCH4V TGCA 3 cut(s) 4, 104, 757
HpyF3I CTNAG 1 cut(s) 787
Hsp92II CATG 3 cut(s) 356, 509, 627
Kzo9I GATC 5 cut(s) 30, 73, 271, 574, 649
LmnI GCTCC 4 cut(s) 413, 502, 620, 765
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 358
MalI GATC 5 cut(s) 32, 75, 273, 576, 651
MboI GATC 5 cut(s) 30, 73, 271, 574, 649
MboII GAAGA 2 cut(s) 68, 445
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 7 cut(s) 23, 192, 238, 387, 588, 659, 728
MlyI GAGTC 2 cut(s) 147, 296
MmeI TCCRAC 2 cut(s) 112, 321
MnlI CCTC 9 cut(s) 30, 44, 56, 143, 515, 532, 543, 602, 623
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 2 cut(s) 510, 570
MspI CCGG 2 cut(s) 231, 266
MspR9I CCNGG 1 cut(s) 412
MvaI CCWGG 1 cut(s) 412
NdeII GATC 5 cut(s) 30, 73, 271, 574, 649
NlaIII CATG 3 cut(s) 356, 509, 627
NlaIV GGNNCC 2 cut(s) 616, 651
NspI RCATGY 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 570
PaeR7I CTCGAG 1 cut(s) 534
PflMI CCANNNNNTGG 1 cut(s) 624
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 2 cut(s) 147, 295
PpsI GAGTC 2 cut(s) 147, 295
Psp124BI GAGCTC 1 cut(s) 783
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 616, 651
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 3 cut(s) 335, 671, 699
RsaNI GTAC 3 cut(s) 334, 670, 698
RseI CAYNNNNRTG 2 cut(s) 510, 570
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 5 cut(s) 30, 73, 271, 574, 649
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 147, 296
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 783
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 534
SgrAI CRCCGGYG 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 510, 570
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
Sse9I AATT 7 cut(s) 23, 192, 238, 387, 588, 659, 728
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 3 cut(s) 329, 618, 682
TaaI ACNGT 2 cut(s) 395, 584
TaqI TCGA 1 cut(s) 535
TasI AATT 7 cut(s) 23, 192, 238, 387, 588, 659, 728
TatI WGTACW 1 cut(s) 669
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 3 cut(s) 313, 577, 610
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 412
TspRI CASTG 3 cut(s) 313, 577, 610
Van91I CCANNNNNTGG 1 cut(s) 624
XceI RCATGY 1 cut(s) 509
XhoI CTCGAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.