MD13G1016100.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008208
Physical Location & Seq
Forward (+)
1028992 .. 1031649
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1016100.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGAGGGTTCTTTTAGAAAAGCTGATACTATTGGGAACTTCAGGTCGTATATATATAGCTCTTCATCAAAGCCACTTGACTCAATTCTCTCAGAGTTTTCTGCGGAAAACTCAAACTCTCGTGGCTTCTTTGTCTAATACTTCTAATAATCATAAAGCCATGATTTCCTCCTTTCACTTCAAGGGATGCCTCCTGTCACTTTGTTTGGTTGCTTGCGCAGCTGAGCTTCAACGTTTTAAGCAGGCACCAAAACCAGATGGCTCTCTCAGCTTCCTGGTTGTCGGAGATTGGGGAAGACGGGGACAATACAACCAATCTCAAGTTGCTCTCCAGATGGGGTTAATTGGAGAGAAATCAAACATAGACTTTGTCATCTCCACTGGTGATAATTTCTACGAAGATGGTTTGACAGGCGTCGACGATCCAGCATTTAACGAATCGTTTTCCAATATTTATACTGCTCCCAGCTTAAAGAAGCAATGGTACAATGTGTTGGGCAACCATGACTATCGGGGTGATGTTGAAGCACAATTGAGTCCCGTTCTCAGGGCAATTGACAGTAGATGGTTTTGCTTAAGATCATTCATTTTAGATGCAGAAATCGTAGATTTTTTCTTTGTGGACACAACCCCATTTGTGGATGATTACTTTACAAATCCAAAGGAGCATACTTATGATTGGAGAGGTGTGACTCCTCGGGCGGAATACCTTTCAAATCTCTTGAAGGATGTGGATTCAGCACTAAAAAATTCCACAGCAAAATGGAAAATTGTGGTCGGTCACCATACTATTAAAAGCGCAGGACATCATGGTGTCACCGAAGAACTTGTAAACCAACTTCTCCCAATCCTTAAGTCAAATAATATCGATTTTTACGTAAATGGACATGACCATTGCTTGGAGCACATAAGTGATTCTGATAGTCAAATTCAGTTCTTTACAAGTGGAGGTGGGTCCAAGGCATGGAGAGGTGATATAAAGTCGTGGAGTCCAGAGGAGTTGAAGTTGTATTACGACGGACAAGGTTTCATGTCGTTTCACATGTCCAAGGCCAGTGCTGATGTTGCATACTATGATGTCTCTGGCAATGTCTTACACAAATGGAGCCTTTCCAAAGAGATCGACTCAGCCACTCACAGAACATTCACCTACTCTTAA

Protein Analysis

386

Amino Acids

43.41

Weight (kDa)

6.23

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 90 - 299 3.6e-18 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 244
AccB7I CCANNNNNTGG 2 cut(s) 898, 963
AccI GTMKAC 1 cut(s) 417
AciI CCGC 2 cut(s) 103, 701
AclI AACGTT 1 cut(s) 232
AclWI GGATC 1 cut(s) 416
AcsI RAATTY 2 cut(s) 748, 927
AcuI CTGAAG 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 4 cut(s) 546, 637, 898, 963
AflII CTTAAG 2 cut(s) 574, 851
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 6 cut(s) 181, 230, 524, 714, 724, 1003
AjnI CCWGG 1 cut(s) 273
AleI CACNNNNGTG 1 cut(s) 909
AluBI AGCT 6 cut(s) 22, 59, 221, 226, 270, 468
AluI AGCT 6 cut(s) 22, 59, 221, 226, 270, 468
Alw21I GWGCWC 1 cut(s) 906
Alw26I GTCTC 1 cut(s) 1084
AlwI GGATC 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 696
AoxI GGCC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 2 cut(s) 748, 927
AspLEI GCGC 2 cut(s) 218, 800
AspS9I GGNCC 1 cut(s) 954
AsuHPI GGTGA 6 cut(s) 395, 527, 773, 808, 983, 1138
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 1 cut(s) 954
BanI GGYRCC 1 cut(s) 244
BarI GAAGNNNNNNTAC 6 cut(s) 467, 499, 822, 854, 995, 1027
BauI CACGAG 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 906
BbvI GCAGC 1 cut(s) 230
BccI CCATC 4 cut(s) 251, 328, 395, 558
BciT130I CCWGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 1084
BfrI CTTAAG 2 cut(s) 574, 851
BisI GCNGC 1 cut(s) 219
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 1 cut(s) 954
BmeT110I CYCGRG 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 954
BmiI GGNNCC 3 cut(s) 246, 955, 1106
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 2 cut(s) 176, 583
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 301
BplI GAGNNNNNCTC 2 cut(s) 1109, 1141
BpmI CTGGAG 1 cut(s) 314
Bpu1102I GCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 877
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 3 cut(s) 695, 957, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 989, 1019
Bsc4I CCNNNNNNNGG 4 cut(s) 546, 637, 898, 963
Bse1I ACTGG 2 cut(s) 385, 1053
Bse3DI GCAATG 3 cut(s) 485, 892, 1093
BseBI CCWGG 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 867
BseDI CCNNGG 3 cut(s) 695, 957, 1047
BseGI GGATG 3 cut(s) 191, 646, 733
BseLI CCNNNNNNNGG 4 cut(s) 546, 637, 898, 963
BseMI GCAATG 3 cut(s) 485, 892, 1093
BseMII CTCAG 5 cut(s) 104, 213, 280, 559, 1140
BseNI ACTGG 2 cut(s) 385, 1053
BseRI GAGGAG 2 cut(s) 684, 1010
BseXI GCAGC 1 cut(s) 230
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 1 cut(s) 1052
BshNI GGYRCC 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 867
BsiHKAI GWGCWC 1 cut(s) 906
BsiHKCI CYCGRG 1 cut(s) 696
BslFI GGGAC 2 cut(s) 315, 522
BslI CCNNNNNNNGG 4 cut(s) 546, 637, 898, 963
BsmAI GTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 315, 522
BsnI GGCC 1 cut(s) 1052
BsoBI CYCGRG 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 906
Bsp143I GATC 3 cut(s) 421, 578, 1119
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 2 cut(s) 103, 701
BspANI GGCC 1 cut(s) 1052
BspCNI CTCAG 5 cut(s) 103, 214, 279, 558, 1139
BspDI ATCGAT 1 cut(s) 867
BspLI GGNNCC 3 cut(s) 246, 955, 1106
BspPI GGATC 1 cut(s) 416
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 244
BspTI CTTAAG 2 cut(s) 574, 851
BsrDI GCAATG 3 cut(s) 485, 892, 1093
BsrI ACTGG 2 cut(s) 385, 1053
BssECI CCNNGG 3 cut(s) 695, 957, 1047
BssMI GATC 3 cut(s) 421, 578, 1119
BssNI GRCGYC 1 cut(s) 414
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 2 cut(s) 957, 1047
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 560
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 414
BstAFI CTTAAG 2 cut(s) 574, 851
BstBAI YACGTR 1 cut(s) 877
BstC8I GCNNGC 2 cut(s) 214, 243
BstDEI CTNAG 5 cut(s) 90, 222, 266, 545, 1126
BstEII GGTNACC 1 cut(s) 779
BstF5I GGATG 3 cut(s) 191, 646, 733
BstHHI GCGC 2 cut(s) 218, 800
BstKTI GATC 3 cut(s) 424, 581, 1122
BstMAI GTCTC 1 cut(s) 1084
BstMBI GATC 3 cut(s) 421, 578, 1119
BstMWI GCNNNNNNNGC 3 cut(s) 218, 267, 1064
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 1045
BstPAI GACNNNNGTC 1 cut(s) 413
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 1 cut(s) 273
BstSNI TACGTA 1 cut(s) 877
BstV1I GCAGC 1 cut(s) 230
BstV2I GAAGAC 1 cut(s) 301
Bsu15I ATCGAT 1 cut(s) 867
BsuRI GGCC 1 cut(s) 1052
BsuTUI ATCGAT 1 cut(s) 867
BtsCI GGATG 3 cut(s) 191, 646, 733
BtsIMutI CAGTG 2 cut(s) 378, 1060
Cac8I GCNNGC 2 cut(s) 214, 243
CfoI GCGC 2 cut(s) 218, 800
Cfr13I GGNCC 1 cut(s) 954
ClaI ATCGAT 1 cut(s) 867
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 484
CviAII CATG 7 cut(s) 160, 503, 809, 887, 963, 1030, 1042
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 5 cut(s) 90, 222, 266, 545, 1126
DpnI GATC 3 cut(s) 423, 580, 1121
DpnII GATC 3 cut(s) 421, 578, 1119
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EciI GGCGGA 1 cut(s) 716
Eco105I TACGTA 1 cut(s) 877
Eco130I CCWWGG 2 cut(s) 957, 1047
Eco47I GGWCC 1 cut(s) 954
Eco57I CTGAAG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 696
Eco91I GGTNACC 1 cut(s) 779
EcoO65I GGTNACC 1 cut(s) 779
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 2 cut(s) 957, 1047
ErhI CCWWGG 2 cut(s) 957, 1047
FaeI CATG 7 cut(s) 163, 506, 812, 890, 966, 1033, 1045
FaqI GGGAC 2 cut(s) 315, 522
FatI CATG 7 cut(s) 159, 502, 808, 886, 962, 1029, 1041
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 3 cut(s) 198, 653, 740
Fsp4HI GCNGC 1 cut(s) 219
FspI TGCGCA 1 cut(s) 217
GlaI GCGC 2 cut(s) 217, 799
GluI GCNGC 1 cut(s) 219
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 1 cut(s) 1052
HgaI GACGC 1 cut(s) 403
HhaI GCGC 2 cut(s) 218, 800
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 7 cut(s) 163, 506, 812, 890, 966, 1033, 1045
Hin6I GCGC 2 cut(s) 216, 798
HinP1I GCGC 2 cut(s) 216, 798
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 8 cut(s) 79, 437, 535, 691, 734, 914, 988, 1124
HphI GGTGA 6 cut(s) 395, 527, 773, 808, 983, 1138
Hpy166II GTNNAC 3 cut(s) 418, 622, 832
Hpy188I TCNGA 3 cut(s) 93, 284, 919
Hpy188III TCNNGA 3 cut(s) 331, 721, 992
Hpy8I GTNNAC 3 cut(s) 418, 622, 832
Hpy99I CGWCG 3 cut(s) 419, 422, 1019
HpyAV CCTTC 1 cut(s) 718
HpyCH4III ACNGT 1 cut(s) 560
HpyCH4IV ACGT 2 cut(s) 232, 876
HpyCH4V TGCA 2 cut(s) 596, 1067
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 267, 1064
HpyF3I CTNAG 5 cut(s) 90, 222, 266, 545, 1126
HpySE526I ACGT 2 cut(s) 232, 876
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 7 cut(s) 163, 506, 812, 890, 966, 1033, 1045
HspAI GCGC 2 cut(s) 216, 798
Kzo9I GATC 3 cut(s) 421, 578, 1119
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 4 cut(s) 466, 664, 901, 1104
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 2 cut(s) 176, 583
MaeII ACGT 2 cut(s) 232, 876
MaeIII GTNAC 4 cut(s) 195, 688, 779, 814
MalI GATC 3 cut(s) 423, 580, 1121
MboI GATC 3 cut(s) 421, 578, 1119
MboII GAAGA 4 cut(s) 53, 306, 410, 833
MfeI CAATTG 2 cut(s) 530, 552
MhlI GDGCHC 1 cut(s) 906
MluCI AATT 8 cut(s) 83, 342, 388, 530, 552, 748, 768, 927
MlyI GAGTC 5 cut(s) 73, 544, 685, 997, 1118
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 7 cut(s) 178, 200, 677, 705, 941, 962, 988
MseI TTAA 8 cut(s) 237, 341, 432, 470, 575, 792, 852, 1156
MslI CAYNNNNRTG 3 cut(s) 672, 810, 909
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 2 cut(s) 574, 851
MspR9I CCNGG 1 cut(s) 275
MunI CAATTG 2 cut(s) 530, 552
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 218, 267, 1064
NdeII GATC 3 cut(s) 421, 578, 1119
NlaIII CATG 7 cut(s) 163, 506, 812, 890, 966, 1033, 1045
NlaIV GGNNCC 3 cut(s) 246, 955, 1106
NmuCI GTSAC 4 cut(s) 195, 688, 779, 814
NsbI TGCGCA 1 cut(s) 217
NspI RCATGY 1 cut(s) 1045
OliI CACNNNNGTG 1 cut(s) 909
PciI ACATGT 1 cut(s) 1041
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 873
PfeI GAWTC 3 cut(s) 437, 734, 914
PflFI GACNNNGTC 1 cut(s) 368
PflMI CCANNNNNTGG 2 cut(s) 898, 963
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 5 cut(s) 73, 543, 685, 996, 1118
PpsI GAGTC 5 cut(s) 73, 543, 685, 996, 1118
Ppu21I YACGTR 1 cut(s) 877
PscI ACATGT 1 cut(s) 1041
PshAI GACNNNNGTC 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 273
PspEI GGTNACC 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 3 cut(s) 246, 955, 1106
PspPI GGNCC 1 cut(s) 954
PsyI GACNNNGTC 1 cut(s) 368
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 3 cut(s) 672, 810, 909
SalI GTCGAC 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 8 cut(s) 237, 341, 432, 470, 575, 792, 852, 1156
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 3 cut(s) 421, 578, 1119
Sau96I GGNCC 1 cut(s) 954
SchI GAGTC 5 cut(s) 73, 544, 685, 997, 1118
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 906
SfaNI GCATC 2 cut(s) 176, 583
SgrDI CGTCGACG 1 cut(s) 416
SinI GGWCC 1 cut(s) 954
SmiMI CAYNNNNRTG 3 cut(s) 672, 810, 909
SmlI CTYRAG 3 cut(s) 318, 574, 851
SmoI CTYRAG 3 cut(s) 318, 574, 851
SnaBI TACGTA 1 cut(s) 877
Sse9I AATT 8 cut(s) 83, 342, 388, 530, 552, 748, 768, 927
SsiI CCGC 2 cut(s) 103, 701
SspI AATATT 1 cut(s) 451
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 2 cut(s) 957, 1047
TaaI ACNGT 1 cut(s) 560
TaiI ACGT 2 cut(s) 235, 879
TaqI TCGA 3 cut(s) 417, 867, 1122
TaqII GACCGA 1 cut(s) 767
TasI AATT 8 cut(s) 83, 342, 388, 530, 552, 748, 768, 927
TfiI GAWTC 3 cut(s) 437, 734, 914
Tru1I TTAA 8 cut(s) 237, 341, 432, 470, 575, 792, 852, 1156
Tru9I TTAA 8 cut(s) 237, 341, 432, 470, 575, 792, 852, 1156
TscAI CASTG 2 cut(s) 385, 1060
TseFI GTSAC 4 cut(s) 195, 688, 779, 814
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 4 cut(s) 195, 688, 779, 814
TspDTI ATGAA 3 cut(s) 53, 574, 1018
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 2 cut(s) 385, 1060
Tth111I GACNNNGTC 1 cut(s) 368
Van91I CCANNNNNTGG 2 cut(s) 898, 963
Vha464I CTTAAG 2 cut(s) 574, 851
VpaK11BI GGWCC 1 cut(s) 954
XapI RAATTY 2 cut(s) 748, 927
XceI RCATGY 1 cut(s) 1045
XmiI GTMKAC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.