RLG00000006047

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008208
Physical Location & Seq
Reverse (-)
3859530 .. 3861976
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006047

Sequence Viewer

Length: 1056 bp
ATGGCTTCTTTGTACTCTTCGAATTCCAACAAAACCATGCAGCTTTTATCCTTCCGACGTCAGTTCATGATCCTCAAGGCTTGTCTCTCACTTTGTTTGGTTGCTAAAGCTGAGTTTCAACGTTTGCAACAGCAAACAAACGACGAAGGGTCTCTGAAATTCTTCGTTGTTGGAGATTGGGGCAGACGAGGACTCTACAACCAATCTCAAGTTGCAGCCCAGATGGGATTAATCGCGACCAAAGAAAACATAGACATTGTTTTCTCCACCGGTGACAATTTCTACGACGATGGTTTGAAAGGCGTTGATGATCCAGCTTTTAAAGAATCATTCTCCAACATCTACAATGCCCAAAGCTTAAAGAAACCATATTGGTTCAGTGTATTGGGTAACCATGACTATCGTGGTAACGTTCTAGCACAACTGAATCCACTCCTCAAACGCATTGACAGTAGATGGTTTTGCTATAGATCATTCCATATCGGTTATGCAGATTTCGTAGAGATTTTCTCTGTGGACACAACCCCATTTGTAGATGGGTACTTCACGGATCCAAAGGAGCATGTCTATGACTGGAGAGGTGTGCTTCCTCGGGAGGATTACCTTTCAAATGTCTTGAAGGTAAGGTCTGGATCATGCACTGAAAAATTCCTCTGCAAAATGGAAATTGTGATCGGTCACCATACAATTAGAAGTGCAGGACATCATGGTAACACCGCTGAGCTTGTAACCAAACTTCTCCCAATCCTTAAGGCAAACGATATAGATTTTTATATAAACGGACATGACCATTGCTTGCAGCACATCAGTGATACCGACAGTAAAATTGAGTTTTTTACCAGTGGAGGTGGCTCCAAGGCATGGAGAGGTGATATAGATCCCTGGGATCCAAGAGAATTGAAGCTGTACTACGACGGCCAAGGTTTTTTGTCGGCCAAAATAACTAAAACCAACGCTGATATACAATTTTACGATGTCTCTGGCAATGTTTTGCACAATTGGAGCAAATCCAAGGTCCTAGACTCGGTCACGCATAGAAAGTTTGTCTATGCTTAA

Protein Analysis

352

Amino Acids

40.11

Weight (kDa)

7.18

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 52 - 265 6.5e-14 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 61
AccB7I CCANNNNNTGG 1 cut(s) 861
AccII CGCG 1 cut(s) 236
AciI CCGC 1 cut(s) 717
AclI AACGTT 2 cut(s) 121, 411
AclWI GGATC 8 cut(s) 64, 305, 545, 558, 640, 872, 881, 894
AcoI YGGCCR 2 cut(s) 916, 933
AcsI RAATTY 3 cut(s) 22, 158, 647
AcyI GRCGYC 1 cut(s) 58
AfaI GTAC 3 cut(s) 14, 542, 908
AfiI CCNNNNNNNGG 2 cut(s) 861, 1024
AflII CTTAAG 1 cut(s) 749
AgeI ACCGGT 1 cut(s) 269
AgsI TTSAA 5 cut(s) 119, 298, 609, 619, 901
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 881
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 6 cut(s) 43, 110, 317, 357, 724, 904
AluI AGCT 6 cut(s) 43, 110, 317, 357, 724, 904
Alw26I GTCTC 3 cut(s) 89, 156, 982
AlwI GGATC 8 cut(s) 64, 305, 545, 558, 640, 872, 881, 894
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 2 cut(s) 916, 933
ApeKI GCWGC 3 cut(s) 40, 215, 799
ApoI RAATTY 3 cut(s) 22, 158, 647
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
AseI ATTAAT 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 1015
AsuHPI GGTGA 3 cut(s) 284, 671, 881
AsuII TTCGAA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 1 cut(s) 1015
BamHI GGATCC 2 cut(s) 550, 886
BarI GAAGNNNNNNTAC 4 cut(s) 720, 752, 893, 925
BbvI GCAGC 3 cut(s) 52, 227, 811
BccI CCATC 4 cut(s) 217, 284, 450, 530
BceAI ACGGC 1 cut(s) 931
BciT130I CCWGG 1 cut(s) 883
BcoDI GTCTC 3 cut(s) 89, 156, 982
BfaI CTAG 2 cut(s) 416, 1019
BfmI CTRYAG 1 cut(s) 466
BfrI CTTAAG 1 cut(s) 749
BisI GCNGC 3 cut(s) 41, 216, 800
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 3 cut(s) 42, 217, 801
Bme1390I CCNGG 1 cut(s) 883
Bme18I GGWCC 1 cut(s) 1015
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 1015
BmiI GGNNCC 3 cut(s) 552, 853, 888
BmrFI CCNGG 1 cut(s) 883
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 595
Bpu1102I GCTNAGC 1 cut(s) 720
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 59, 192
BsaBI GATNNNNATC 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 58
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 6 cut(s) 590, 855, 881, 882, 919, 1011
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 2 cut(s) 861, 1024
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 578, 840
Bse3DI GCAATG 2 cut(s) 790, 991
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 6 cut(s) 590, 855, 881, 882, 919, 1011
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 2 cut(s) 861, 1024
BseMI GCAATG 2 cut(s) 790, 991
BseMII CTCAG 2 cut(s) 102, 711
BseNI ACTGG 2 cut(s) 578, 840
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 52, 227, 811
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 918, 935
BshTI ACCGGT 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 1 cut(s) 270
BslI CCNNNNNNNGG 2 cut(s) 861, 1024
BsmAI GTCTC 3 cut(s) 89, 156, 982
BsnI GGCC 2 cut(s) 918, 935
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 591
Bsp119I TTCGAA 1 cut(s) 20
Bsp143I GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
Bsp1720I GCTNAGC 1 cut(s) 720
Bsp68I TCGCGA 1 cut(s) 236
BspACI CCGC 1 cut(s) 717
BspANI GGCC 2 cut(s) 918, 935
BspCNI CTCAG 2 cut(s) 103, 712
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 552, 853, 888
BspPI GGATC 8 cut(s) 64, 305, 545, 558, 640, 872, 881, 894
BspT104I TTCGAA 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 790, 991
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 2 cut(s) 578, 840
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 590, 855, 881, 882, 919, 1011
BssMI GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
BssNI GRCGYC 1 cut(s) 58
BssT1I CCWWGG 3 cut(s) 855, 919, 1011
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 2 cut(s) 452, 821
Bst6I CTCTTC 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 58
BstAFI CTTAAG 1 cut(s) 749
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 797
BstDEI CTNAG 2 cut(s) 111, 720
BstEII GGTNACC 2 cut(s) 389, 677
BstFNI CGCG 1 cut(s) 236
BstKTI GATC 8 cut(s) 72, 313, 473, 553, 635, 675, 880, 889
BstMAI GTCTC 3 cut(s) 89, 156, 982
BstMBI GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 1 cut(s) 566
BstPI GGTNACC 2 cut(s) 389, 677
BstSCI CCNGG 1 cut(s) 881
BstSFI CTRYAG 1 cut(s) 466
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 3 cut(s) 52, 227, 811
BstX2I RGATCY 3 cut(s) 550, 877, 886
BstYI RGATCY 3 cut(s) 550, 877, 886
BsuRI GGCC 2 cut(s) 918, 935
BtsIMutI CAGTG 4 cut(s) 385, 639, 814, 847
BtuMI TCGCGA 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 797
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 1015
Csp6I GTAC 3 cut(s) 13, 541, 907
CspAI ACCGGT 1 cut(s) 269
CviAII CATG 8 cut(s) 37, 67, 395, 563, 636, 707, 785, 861
CviQI GTAC 3 cut(s) 13, 541, 907
DdeI CTNAG 2 cut(s) 111, 720
DpnI GATC 8 cut(s) 71, 312, 472, 552, 634, 674, 879, 888
DpnII GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
DraI TTTAAA 1 cut(s) 322
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 916, 933
Eam1104I CTCTTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 855, 919, 1011
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 1015
Eco88I CYCGRG 1 cut(s) 591
Eco91I GGTNACC 2 cut(s) 389, 677
EcoO109I RGGNCCY 1 cut(s) 1015
EcoO65I GGTNACC 2 cut(s) 389, 677
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 881
EcoT14I CCWWGG 3 cut(s) 855, 919, 1011
ErhI CCWWGG 3 cut(s) 855, 919, 1011
FaeI CATG 8 cut(s) 40, 70, 398, 566, 639, 710, 788, 864
FatI CATG 8 cut(s) 36, 66, 394, 562, 635, 706, 784, 860
Fnu4HI GCNGC 3 cut(s) 41, 216, 800
Fsp4HI GCNGC 3 cut(s) 41, 216, 800
FspBI CTAG 2 cut(s) 416, 1019
GluI GCNGC 3 cut(s) 41, 216, 800
GsuI CTGGAG 1 cut(s) 595
HaeIII GGCC 2 cut(s) 918, 935
HapII CCGG 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 8 cut(s) 40, 70, 398, 566, 639, 710, 788, 864
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 4 cut(s) 192, 326, 427, 1022
HpaII CCGG 1 cut(s) 270
HphI GGTGA 3 cut(s) 284, 671, 881
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 56, 156
Hpy188III TCNNGA 5 cut(s) 67, 235, 593, 616, 630
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 4 cut(s) 60, 146, 290, 917
HpyAV CCTTC 3 cut(s) 61, 140, 613
HpyCH4III ACNGT 2 cut(s) 452, 821
HpyCH4IV ACGT 3 cut(s) 58, 121, 411
HpyCH4V TGCA 9 cut(s) 40, 127, 215, 491, 639, 657, 698, 799, 994
HpyF3I CTNAG 2 cut(s) 111, 720
HpySE526I ACGT 3 cut(s) 58, 121, 411
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 8 cut(s) 40, 70, 398, 566, 639, 710, 788, 864
Kzo9I GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
LmnI GCTCC 3 cut(s) 559, 857, 1002
Lsp1109I GCAGC 3 cut(s) 52, 227, 811
MaeI CTAG 2 cut(s) 416, 1019
MaeII ACGT 3 cut(s) 58, 121, 411
MaeIII GTNAC 7 cut(s) 272, 389, 407, 677, 710, 727, 1027
MalI GATC 8 cut(s) 71, 312, 472, 552, 634, 674, 879, 888
MboI GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
MboII GAAGA 2 cut(s) 9, 154
MfeI CAATTG 1 cut(s) 997
MflI RGATCY 3 cut(s) 550, 877, 886
MlyI GAGTC 2 cut(s) 186, 1016
MmeI TCCRAC 4 cut(s) 51, 79, 151, 360
MnlI CCTC 9 cut(s) 83, 182, 446, 572, 589, 600, 662, 839, 860
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 5 cut(s) 230, 321, 359, 750, 1054
MslI CAYNNNNRTG 2 cut(s) 567, 807
MspA1I CMGCKG 1 cut(s) 719
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 883
MunI CAATTG 1 cut(s) 997
MvaI CCWGG 1 cut(s) 883
MvnI CGCG 1 cut(s) 236
NdeII GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
NlaIII CATG 8 cut(s) 40, 70, 398, 566, 639, 710, 788, 864
NlaIV GGNNCC 3 cut(s) 552, 853, 888
NmuCI GTSAC 3 cut(s) 272, 677, 1027
NruI TCGCGA 1 cut(s) 236
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 20
OliI CACNNNNGTG 1 cut(s) 807
PagI TCATGA 1 cut(s) 66
PasI CCCWGGG 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 326, 427
PflFI GACNNNGTC 1 cut(s) 1025
PflMI CCANNNNNTGG 1 cut(s) 861
PinAI ACCGGT 1 cut(s) 269
PkrI GCNGC 3 cut(s) 42, 217, 801
PleI GAGTC 2 cut(s) 186, 1016
PpsI GAGTC 2 cut(s) 186, 1016
PpuMI RGGWCCY 1 cut(s) 1015
PshBI ATTAAT 1 cut(s) 230
Psp1406I AACGTT 2 cut(s) 121, 411
Psp5II RGGWCCY 1 cut(s) 1015
Psp6I CCWGG 1 cut(s) 881
PspEI GGTNACC 2 cut(s) 389, 677
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 3 cut(s) 552, 853, 888
PspPI GGNCC 1 cut(s) 1015
PspPPI RGGWCCY 1 cut(s) 1015
PsuI RGATCY 3 cut(s) 550, 877, 886
PsyI GACNNNGTC 1 cut(s) 1025
RruI TCGCGA 1 cut(s) 236
RsaI GTAC 3 cut(s) 14, 542, 908
RsaNI GTAC 3 cut(s) 13, 541, 907
RseI CAYNNNNRTG 2 cut(s) 567, 807
SaqAI TTAA 5 cut(s) 230, 321, 359, 750, 1054
SatI GCNGC 3 cut(s) 41, 216, 800
Sau3AI GATC 8 cut(s) 69, 310, 470, 550, 632, 672, 877, 886
Sau96I GGNCC 1 cut(s) 1015
SchI GAGTC 2 cut(s) 186, 1016
ScrFI CCNGG 1 cut(s) 883
SfcI CTRYAG 1 cut(s) 466
SfuI TTCGAA 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 1 cut(s) 1015
SmiMI CAYNNNNRTG 2 cut(s) 567, 807
SmlI CTYRAG 3 cut(s) 74, 207, 749
SmoI CTYRAG 3 cut(s) 74, 207, 749
SsiI CCGC 1 cut(s) 717
SspMI CTAG 2 cut(s) 416, 1019
StyD4I CCNGG 1 cut(s) 881
StyI CCWWGG 3 cut(s) 855, 919, 1011
TaaI ACNGT 2 cut(s) 452, 821
TaiI ACGT 3 cut(s) 61, 124, 414
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 2 cut(s) 665, 1015
TatI WGTACW 2 cut(s) 12, 906
TfiI GAWTC 2 cut(s) 326, 427
Tru1I TTAA 5 cut(s) 230, 321, 359, 750, 1054
Tru9I TTAA 5 cut(s) 230, 321, 359, 750, 1054
TscAI CASTG 4 cut(s) 385, 646, 814, 847
TseFI GTSAC 3 cut(s) 272, 677, 1027
TseI GCWGC 3 cut(s) 40, 215, 799
Tsp45I GTSAC 3 cut(s) 272, 677, 1027
TspDTI ATGAA 1 cut(s) 55
TspGWI ACGGA 2 cut(s) 563, 795
TspRI CASTG 4 cut(s) 385, 646, 814, 847
Tth111I GACNNNGTC 1 cut(s) 1025
Van91I CCANNNNNTGG 1 cut(s) 861
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 1015
VspI ATTAAT 1 cut(s) 230
XapI RAATTY 3 cut(s) 22, 158, 647
XceI RCATGY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 416, 1019
ZraI GACGTC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.