Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003211

Physical Location & Seq

Genomic Coordinates Forward (+)

32601257 .. 32604857

Gene Structure 3601 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g35240.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGGCCGGCGCTTTACATCTCATCCTTAACCGGCCATGGCTTCCTCTTCTTCTTCTGTTTTGTATTTTCCGTTTTTGTTATGCTCAACCACAAAACATCGAGACTTTCTTTCCTTTTCCACCAGTGCCGTCCCTGGCACCAGCGCCGTCCCCGGCGCCGATCTCTCCGTATTTTCCTCCGGTGGAGTCGCCTCCACCGGAGCCGCAACCTCCTCTGCCGCTGCCGTTACCGAATAAGAAGTCCAATGACAACAGAAACATTGCAAAGGCGGCAGCGGCAACCGCAGCCAGCACACTGGTGTTGTGTGGGGTGGTGTTCTTTTTCGTGCAAAGGTGCATTGTTGCTAAGCGGAGGAGGAACAAGATAGGCGGTGGCGCTGTTATTGGTGGTGGTGGTGCTTATAACAATACTAGCTCATCACGTCGTGGTCAACCGGCTGCAGTGGTCAATCGTAATGAGTTTGAGAGGTATGATGGGAATCTCAAAGGGTTCATTGTGGACGAGGATGGTCTTGATGTGTTGTATTGGCGAAAACTTGAGGCCAAGAACTCGAAGAAGAAGAGTTTCAAGAAAGAGGCTATGCGAAACCGGGATGCAGGAGAAGAAGAAATTGATGAAGATCATAGTGGAGAAGGGACTAGCAGAGAGAATGAGCTTCCTCTGTTTCGAGGGAGGTCTTCCACGTCTAACATGGATTTAGTGCCCGAAGGAAATGAGCAGATTCGAATGGGAAGTAGTAGAATGATGGCTCCCAAGGCTGTTGAGAATCGAGAGCAAGTAGCAGAGCTGACGTTTCAGTCTTCGGGTTCTCCTCCTCCACCTCATTCTCCACCACCAACCCCACCTCCTCTGCCAATGAAAGTCCCTGCAGTGCCGCTACCGCCGCCTCCACCGCCTGTGAAGCGCCCGGGACTGCCGCCGGCTCCGCCACCGAAGCCTAAGTCAAGAAGTTTAAATGCAATGGCAAAACCAGAGCCAAAGGGGGTGAAGCTGAAGCCACTACATTGGGACAAGGTGAACACTAATCATGAACATTCCATGGTGTGGGACAAAATTGATAATGGCTCCTTCAGATTTGATGGTGACCTTATGGAAGCTCTTTTTGGAACTGTTGCAACTAATCGGAAATCTCCTGAAAGAGTAACTGGTTCATCATCGTCACAAGTTTGTATCTTAGATGAGAGGAAGTCTCAGAATTTTGCAATTGTTATAAAGTCATTGACCATTTCTATTGAAGATATTCTTGATGTGCTTAATGAGGGCAGAGGTTTAGGCGCTGAAAATCTCGAAAAGCTTGCTAGAATTGCACCAACTGAAGAGGAAATAGCCAAAATTCTTGAGTACAATGAAGACCCTGCAAGGCTTGCTGATGCTGAAAGCTTCCTCTACCACATTCTGAAAGCTGTTCCATCGGCTTTTATTCGACTCAATGCCATGCATTTCAGACAGCACTATGAGCAAGAGATTGTGCAGCTCAAGGAGTCTTTACAGACGATTGAATTGGGATGTAAGGAGCTTCGAACTAGAGGACTGTTTATGAAGCTGCTAGAAGCGATTCTCAAGGCTGGAAACCGAATGAATGCTGGAACTTCGAGAGGGAATGCTCAAGCTTTCAAGCTCAGTTCTCTTCGAAAACTATCTGATGTTCGAAGTAGTGATGGGAAGACTACTCTTCTACATTTTGTTGTGGAAGAAGTAGTGCGGTCTGAGGGAAAACGGGGTGTTTTGAACAGGAACCATAGCTTGAACCGGAGCGGCAGCCAGAGAAGTAGTAACAGCAACATGAGTTCTGAGCAGAATGTTAAATCGAAAGAGGATAGAGAAAAGGAGTACATGATGTTGGGATTGCCTGTGGTAGGAGGCATCAGTTCTGAGTTCTCTAACGTGAAGAAGGCAGCAACTATAGACTATGACAGCTTTGCAATCACTTGCTCAGCTCTCACTAAACGTGTTGTGGAAACTAGAGAACTTATACTCCAATGTGAGAAGGATGGTGGTGGAAGATTTGTTTTCGAGATGAATGATTTTCTTGAAGAAGCCGAAGAAGAGCTAAAGGAGATGACAAAAGAGCAGAATAGGGTGATGGAGTTTCTGAAGGCTACTACGGAATACTACCAAGGAGGGGCTTCAAAGGAAAAAGGATCACACCCATTTCAACTGTTTGTTGTGGTTAAAGATTTTCTAGGCATGGTTGATCAAGCATGCGTGGAAATTTCTAGAAATGTGCAGAGGAGGAAGGCAGTGACGGCAAGTCTAGAAACATCATCGTCACCGGACTCACCGCCATCAAGAAACCCCGTAAGGTTTCCAGTCTTGCCTAAAAATTTCATGTCAGGAAGCTCTAGAAGTAATTCTAGTGGATCAGACAATGATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005522 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0010638 GO:0016020 GO:0016043 GO:0016049 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0033043 GO:0040007 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048522 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0060560 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2

Protein Analysis

793

Amino Acids

87.64

Weight (kDa)

8.91

Isoelectric Point (pI)

63.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	323 - 731	5.8e-101	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24150 AT1G59910 AT1G70140
fragaria_vesca	FvH4_4g35240 FvH4_5g16231
malus_domestica	MD06G1182500.v1.1 MD13G1085100.v1.1 MD14G1188500.v1.1 MD16G1084300.v1.1
prunus_persica	Prupe.1G261700_v2.0.a1 Prupe.5G180600_v2.0.a1
pyrus_communis	pycom06g16140 pycom13g07360
rosa_chinensis	RchiOBHm_Chr4g0444841 RchiOBHm_Chr7g0179861
rosa_laevigata	RLG00000005317 RLG00000005796
rosa_multiflora	Rmu_sc0000096.1_g000028 Rmu_sc0001754.1_g000001 Rmu_sc0006845.1_g000009 Rmu_sc0031375.1_g000001 Rmu_sc0034891.1_g000002 Rmu_sc0034892.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273390 Rroxscaffold_5G00385330
rosa_rugosa	Rorug04G0355500 Rorug06G0428800
rosa_samantha	Rh4AG417000 Rh4BG428800 Rh4CG443600 Rh4DG424800 Rh7AG028700 Rh7BG027900 Rh7CG029600 Rh7DG029000
rosa_wichuraiana	Rw4G035740 Rw7G002300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	402, 1211
AasI	GACNNNNNNGTC	2 cut(s)	796, 2372
AccB1I	GGYRCC	2 cut(s)	136, 154
AccB7I	CCANNNNNTGG	1 cut(s)	1044
AccBSI	CCGCTC	1 cut(s)	1755
AclWI	GGATC	2 cut(s)	2149, 2368
AcoI	YGGCCR	2 cut(s)	3, 32
AcsI	RAATTY	4 cut(s)	1195, 1332, 2211, 2323
AcuI	CTGAAG	4 cut(s)	1013, 1054, 1335, 2114
AcyI	GRCGYC	1 cut(s)	155
AdeI	CACNNNGTG	1 cut(s)	425
AfaI	GTAC	2 cut(s)	1343, 1832
AfiI	CCNNNNNNNGG	3 cut(s)	1044, 1856, 2122
AflIII	ACRYGT	1 cut(s)	1948
AgsI	TTSAA	9 cut(s)	568, 1235, 1499, 1615, 1729, 1747, 2033, 2130, 2156
AhdI	GACNNNNNGTC	1 cut(s)	2250
AjiI	CACGTC	2 cut(s)	422, 684
AjnI	CCWGG	1 cut(s)	132
AjuI	GAANNNNNNNTTGG	2 cut(s)	1086, 1118
AleI	CACNNNNGTG	1 cut(s)	296
AloI	GAACNNNNNNTCC	2 cut(s)	1959, 1991
Alw26I	GTCTC	2 cut(s)	95, 1194
AlwI	GGATC	2 cut(s)	2149, 2368
Ama87I	CYCGRG	1 cut(s)	907
AoxI	GGCC	3 cut(s)	3, 32, 540
ApeKI	GCWGC	8 cut(s)	220, 272, 284, 437, 1471, 1543, 1758, 1895
ApoI	RAATTY	4 cut(s)	1195, 1332, 2211, 2323
ArsI	GACNNNNNNTTYG	4 cut(s)	224, 256, 926, 958
Asp700I	GAANNNNTTC	4 cut(s)	563, 1239, 1554, 2350
AspLEI	GCGC	6 cut(s)	11, 145, 157, 377, 906, 1277
AsuC2I	CCSGG	4 cut(s)	152, 590, 908, 909
AsuHPI	GGTGA	6 cut(s)	997, 1027, 1094, 2092, 2262, 2271
AsuII	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
AvaI	CYCGRG	1 cut(s)	907
BaeGI	GKGCMC	1 cut(s)	705
BanI	GGYRCC	2 cut(s)	136, 154
BbsI	GAAGAC	4 cut(s)	669, 792, 1356, 1670
BbvI	GCAGC	8 cut(s)	207, 284, 296, 424, 1483, 1530, 1770, 1907
BccI	CCATC	9 cut(s)	467, 500, 739, 1073, 1417, 1652, 1985, 2077, 2293
BceAI	ACGGC	4 cut(s)	112, 130, 208, 2262
BcgI	CGANNNNNNTGC	2 cut(s)	1277, 1311
BciT130I	CCWGG	1 cut(s)	134
BclI	TGATCA	1 cut(s)	2194
BcnI	CCSGG	4 cut(s)	152, 590, 908, 909
BcoDI	GTCTC	2 cut(s)	95, 1194
BfmI	CTRYAG	3 cut(s)	438, 867, 1902
BfoI	RGCGCY	6 cut(s)	12, 146, 158, 378, 907, 1278
BlpI	GCTNAGC	2 cut(s)	345, 1933
Bme1390I	CCNGG	5 cut(s)	134, 152, 590, 908, 909
BmeRI	GACNNNNNGTC	1 cut(s)	2250
BmeT110I	CYCGRG	1 cut(s)	907
BmgBI	CACGTC	2 cut(s)	422, 684
BmiI	GGNNCC	7 cut(s)	138, 156, 201, 750, 924, 1066, 1736
BmrFI	CCNGG	5 cut(s)	134, 152, 590, 908, 909
BmsI	GCATC	3 cut(s)	583, 1360, 1872
BpiI	GAAGAC	4 cut(s)	669, 792, 1356, 1670
BplI	GAGNNNNNCTC	2 cut(s)	1174, 1206
Bpu1102I	GCTNAGC	2 cut(s)	345, 1933
Bpu14I	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
BpuEI	CTTGAG	5 cut(s)	557, 1358, 1460, 1544, 1590
BpuMI	CCSGG	4 cut(s)	152, 590, 908, 909
BsaBI	GATNNNNATC	2 cut(s)	477, 618
BsaHI	GRCGYC	1 cut(s)	155
BsaJI	CCNNGG	7 cut(s)	35, 132, 150, 753, 907, 1038, 2116
BsaWI	WCCGGW	4 cut(s)	178, 196, 1749, 2272
BsaXI	ACNNNNNCTCC	4 cut(s)	829, 859, 1959, 1989
Bsc4I	CCNNNNNNNGG	3 cut(s)	1044, 1856, 2122
Bse118I	RCCGGY	4 cut(s)	5, 30, 433, 919
Bse1I	ACTGG	4 cut(s)	122, 300, 1150, 2309
Bse3DI	GCAATG	2 cut(s)	258, 966
Bse8I	GATNNNNATC	2 cut(s)	477, 618
BseBI	CCWGG	1 cut(s)	134
BseDI	CCNNGG	7 cut(s)	35, 132, 150, 753, 907, 1038, 2116
BseGI	GGATG	5 cut(s)	21, 511, 598, 1511, 1996
BseJI	GATNNNNATC	2 cut(s)	477, 618
BseLI	CCNNNNNNNGG	3 cut(s)	1044, 1856, 2122
BseMI	GCAATG	2 cut(s)	258, 966
BseMII	CTCAG	6 cut(s)	1205, 1633, 1698, 1782, 1863, 1947
BseNI	ACTGG	4 cut(s)	122, 300, 1150, 2309
BseRI	GAGGAG	6 cut(s)	201, 367, 801, 804, 837, 2245
BseSI	GKGCMC	1 cut(s)	705
BseXI	GCAGC	8 cut(s)	207, 284, 296, 424, 1483, 1530, 1770, 1907
BsgI	GTGCAG	2 cut(s)	1490, 2246
BshFI	GGCC	3 cut(s)	5, 34, 542
BshNI	GGYRCC	2 cut(s)	136, 154
BsiHKCI	CYCGRG	1 cut(s)	907
BslFI	GGGAC	7 cut(s)	115, 133, 649, 848, 924, 1022, 1061
BslI	CCNNNNNNNGG	3 cut(s)	1044, 1856, 2122
BsmAI	GTCTC	2 cut(s)	95, 1194
BsmFI	GGGAC	7 cut(s)	115, 133, 649, 848, 924, 1022, 1061
BsmI	GAATGC	2 cut(s)	1585, 1606
BsnI	GGCC	3 cut(s)	5, 34, 542
BsoBI	CYCGRG	1 cut(s)	907
Bsp119I	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
Bsp1286I	GDGCHC	1 cut(s)	705
Bsp143I	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
Bsp1720I	GCTNAGC	2 cut(s)	345, 1933
Bsp19I	CCATGG	2 cut(s)	35, 1038
BspANI	GGCC	3 cut(s)	5, 34, 542
BspCNI	CTCAG	6 cut(s)	1204, 1632, 1699, 1783, 1864, 1946
BspHI	TCATGA	1 cut(s)	1027
BspLI	GGNNCC	7 cut(s)	138, 156, 201, 750, 924, 1066, 1736
BspMAI	CTGCAG	2 cut(s)	442, 871
BspPI	GGATC	2 cut(s)	2149, 2368
BspQI	GCTCTTC	1 cut(s)	2040
BspT104I	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
BspT107I	GGYRCC	2 cut(s)	136, 154
BsrBI	CCGCTC	1 cut(s)	1755
BsrDI	GCAATG	2 cut(s)	258, 966
BsrFI	RCCGGY	4 cut(s)	5, 30, 433, 919
BsrI	ACTGG	4 cut(s)	122, 300, 1150, 2309
BssAI	RCCGGY	4 cut(s)	5, 30, 433, 919
BssECI	CCNNGG	7 cut(s)	35, 132, 150, 753, 907, 1038, 2116
BssMI	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
BssNI	GRCGYC	1 cut(s)	155
BssT1I	CCWWGG	4 cut(s)	35, 753, 1038, 2116
Bst2UI	CCWGG	1 cut(s)	134
Bst4CI	ACNGT	3 cut(s)	1111, 1533, 2160
Bst6I	CTCTTC	6 cut(s)	51, 554, 1311, 1632, 1677, 2040
BstACI	GRCGYC	1 cut(s)	155
BstAPI	GCANNNNNTGC	1 cut(s)	1364
BstBI	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
BstC8I	GCNNGC	6 cut(s)	7, 289, 921, 1296, 1365, 2203
BstDEI	CTNAG	9 cut(s)	345, 939, 1174, 1191, 1619, 1707, 1791, 1872, 1933
BstDSI	CCRYGG	2 cut(s)	35, 1038
BstEII	GGTNACC	1 cut(s)	1082
BstENI	CCTNNNNNAGG	1 cut(s)	1854
BstF5I	GGATG	5 cut(s)	21, 511, 598, 1511, 1996
BstH2I	RGCGCY	6 cut(s)	12, 146, 158, 378, 907, 1278
BstHHI	GCGC	6 cut(s)	11, 145, 157, 377, 906, 1277
BstKTI	GATC	5 cut(s)	162, 622, 2144, 2197, 2363
BstMAI	GTCTC	2 cut(s)	95, 1194
BstMBI	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
BstNI	CCWGG	1 cut(s)	134
BstNSI	RCATGY	1 cut(s)	2205
BstPI	GGTNACC	1 cut(s)	1082
BstSCI	CCNGG	5 cut(s)	132, 150, 588, 906, 907
BstSFI	CTRYAG	3 cut(s)	438, 867, 1902
BstSLI	GKGCMC	1 cut(s)	705
BstV1I	GCAGC	8 cut(s)	207, 284, 296, 424, 1483, 1530, 1770, 1907
BstV2I	GAAGAC	4 cut(s)	669, 792, 1356, 1670
BstXI	CCANNNNNNTGG	2 cut(s)	295, 1005
BsuRI	GGCC	3 cut(s)	5, 34, 542
BtgI	CCRYGG	2 cut(s)	35, 1038
BtrI	CACGTC	2 cut(s)	422, 684
BtsCI	GGATG	5 cut(s)	21, 511, 598, 1511, 1996
BtsI	GCAGTG	3 cut(s)	447, 876, 2247
BtsIMutI	CAGTG	5 cut(s)	129, 293, 447, 876, 2247
Cac8I	GCNNGC	6 cut(s)	7, 289, 921, 1296, 1365, 2203
CciI	TCATGA	1 cut(s)	1027
CfoI	GCGC	6 cut(s)	11, 145, 157, 377, 906, 1277
Cfr10I	RCCGGY	4 cut(s)	5, 30, 433, 919
Cfr9I	CCCGGG	1 cut(s)	907
Csp6I	GTAC	2 cut(s)	1342, 1831
CviQI	GTAC	2 cut(s)	1342, 1831
DdeI	CTNAG	9 cut(s)	345, 939, 1174, 1191, 1619, 1707, 1791, 1872, 1933
DinI	GGCGCC	1 cut(s)	156
DpnI	GATC	5 cut(s)	161, 621, 2143, 2196, 2362
DpnII	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
DraI	TTTAAA	1 cut(s)	954
DraIII	CACNNNGTG	1 cut(s)	425
DrdI	GACNNNNNNGTC	2 cut(s)	796, 2372
DriI	GACNNNNNGTC	1 cut(s)	2250
DseDI	GACNNNNNNGTC	2 cut(s)	796, 2372
EaeI	YGGCCR	2 cut(s)	3, 32
Eam1104I	CTCTTC	6 cut(s)	51, 554, 1311, 1632, 1677, 2040
Eam1105I	GACNNNNNGTC	1 cut(s)	2250
EarI	CTCTTC	6 cut(s)	51, 554, 1311, 1632, 1677, 2040
EciI	GGCGGA	1 cut(s)	915
Eco130I	CCWWGG	4 cut(s)	35, 753, 1038, 2116
Eco57I	CTGAAG	4 cut(s)	1013, 1054, 1335, 2114
Eco88I	CYCGRG	1 cut(s)	907
Eco91I	GGTNACC	1 cut(s)	1082
EcoNI	CCTNNNNNAGG	1 cut(s)	1854
EcoO65I	GGTNACC	1 cut(s)	1082
EcoRII	CCWGG	1 cut(s)	132
EcoT14I	CCWWGG	4 cut(s)	35, 753, 1038, 2116
EcoT22I	ATGCAT	1 cut(s)	1440
EgeI	GGCGCC	1 cut(s)	156
EheI	GGCGCC	1 cut(s)	156
ErhI	CCWWGG	4 cut(s)	35, 753, 1038, 2116
FalI	AAGNNNNNCTT	2 cut(s)	1227, 1259
FaqI	GGGAC	7 cut(s)	115, 133, 649, 848, 924, 1022, 1061
FbaI	TGATCA	1 cut(s)	2194
FokI	GGATG	5 cut(s)	8, 518, 605, 1518, 2003
GlaI	GCGC	6 cut(s)	10, 144, 156, 376, 905, 1276
HaeII	RGCGCY	6 cut(s)	12, 146, 158, 378, 907, 1278
HaeIII	GGCC	3 cut(s)	5, 34, 542
HhaI	GCGC	6 cut(s)	11, 145, 157, 377, 906, 1277
Hin1I	GRCGYC	1 cut(s)	155
Hin6I	GCGC	6 cut(s)	9, 143, 155, 375, 904, 1275
HinP1I	GCGC	6 cut(s)	9, 143, 155, 375, 904, 1275
HincII	GTYRAC	1 cut(s)	431
HindII	GTYRAC	1 cut(s)	431
HindIII	AAGCTT	3 cut(s)	1292, 1378, 1608
HinfI	GANTC	8 cut(s)	185, 478, 721, 766, 1425, 1481, 1555, 2276
HphI	GGTGA	6 cut(s)	997, 1027, 1094, 2092, 2262, 2271
Hpy166II	GTNNAC	3 cut(s)	431, 499, 1018
Hpy8I	GTNNAC	3 cut(s)	431, 499, 1018
Hpy99I	CGWCG	1 cut(s)	426
HpyAV	CCTTC	7 cut(s)	626, 701, 1078, 1885, 1981, 2089, 2230
HpyCH4III	ACNGT	3 cut(s)	1111, 1533, 2160
HpyCH4IV	ACGT	5 cut(s)	421, 683, 791, 1884, 1948
HpyF3I	CTNAG	9 cut(s)	345, 939, 1174, 1191, 1619, 1707, 1791, 1872, 1933
HpySE526I	ACGT	5 cut(s)	421, 683, 791, 1884, 1948
Hsp92I	GRCGYC	1 cut(s)	155
HspAI	GCGC	6 cut(s)	9, 143, 155, 375, 904, 1275
KasI	GGCGCC	1 cut(s)	154
KroI	GCCGGC	2 cut(s)	5, 919
KroNI	GCCGGC	2 cut(s)	7, 921
Ksp22I	TGATCA	1 cut(s)	2194
Kzo9I	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
LguI	GCTCTTC	1 cut(s)	2040
LmnI	GCTCC	6 cut(s)	199, 754, 928, 1070, 1513, 1752
Lsp1109I	GCAGC	8 cut(s)	207, 284, 296, 424, 1483, 1530, 1770, 1907
LweI	GCATC	3 cut(s)	583, 1360, 1872
MaeII	ACGT	5 cut(s)	421, 683, 791, 1884, 1948
MaeIII	GTNAC	7 cut(s)	225, 1082, 1141, 1158, 1772, 2242, 2268
MalI	GATC	5 cut(s)	161, 621, 2143, 2196, 2362
MbiI	CCGCTC	1 cut(s)	1755
MboI	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
MfeI	CAATTG	1 cut(s)	1203
MhlI	GDGCHC	1 cut(s)	705
Mly113I	GGCGCC	1 cut(s)	155
MlyI	GAGTC	4 cut(s)	194, 1419, 1490, 2270
Mph1103I	ATGCAT	1 cut(s)	1440
MroNI	GCCGGC	2 cut(s)	5, 919
MroXI	GAANNNNTTC	4 cut(s)	563, 1239, 1554, 2350
MseI	TTAA	5 cut(s)	27, 953, 1254, 1803, 2172
MslI	CAYNNNNRTG	1 cut(s)	296
MspA1I	CMGCKG	2 cut(s)	220, 275
MspR9I	CCNGG	5 cut(s)	134, 152, 590, 908, 909
MunI	CAATTG	1 cut(s)	1203
Mva1269I	GAATGC	2 cut(s)	1585, 1606
MvaI	CCWGG	1 cut(s)	134
NaeI	GCCGGC	2 cut(s)	7, 921
NarI	GGCGCC	1 cut(s)	155
NciI	CCSGG	4 cut(s)	152, 590, 908, 909
NcoI	CCATGG	2 cut(s)	35, 1038
NdeII	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
NgoMIV	GCCGGC	2 cut(s)	5, 919
NlaIV	GGNNCC	7 cut(s)	138, 156, 201, 750, 924, 1066, 1736
NmuCI	GTSAC	4 cut(s)	1082, 1158, 2242, 2268
NsiI	ATGCAT	1 cut(s)	1440
NspI	RCATGY	1 cut(s)	2205
NspV	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
OliI	CACNNNNGTG	1 cut(s)	296
PaeI	GCATGC	1 cut(s)	2205
PagI	TCATGA	1 cut(s)	1027
PciSI	GCTCTTC	1 cut(s)	2040
PctI	GAATGC	2 cut(s)	1585, 1606
PdiI	GCCGGC	2 cut(s)	7, 921
PdmI	GAANNNNTTC	4 cut(s)	563, 1239, 1554, 2350
PfeI	GAWTC	4 cut(s)	478, 721, 766, 1555
PflMI	CCANNNNNTGG	1 cut(s)	1044
PleI	GAGTC	4 cut(s)	193, 1419, 1489, 2270
PluTI	GGCGCC	1 cut(s)	158
PpsI	GAGTC	4 cut(s)	193, 1419, 1489, 2270
PsiI	TTATAA	2 cut(s)	402, 1211
Psp6I	CCWGG	1 cut(s)	132
PspEI	GGTNACC	1 cut(s)	1082
PspGI	CCWGG	1 cut(s)	132
PspN4I	GGNNCC	7 cut(s)	138, 156, 201, 750, 924, 1066, 1736
PstI	CTGCAG	2 cut(s)	442, 871
RsaI	GTAC	2 cut(s)	1343, 1832
RsaNI	GTAC	2 cut(s)	1342, 1831
RseI	CAYNNNNRTG	1 cut(s)	296
SapI	GCTCTTC	1 cut(s)	2040
SaqAI	TTAA	5 cut(s)	27, 953, 1254, 1803, 2172
Sau3AI	GATC	5 cut(s)	159, 619, 2141, 2194, 2360
SchI	GAGTC	4 cut(s)	194, 1419, 1490, 2270
ScrFI	CCNGG	5 cut(s)	134, 152, 590, 908, 909
SduI	GDGCHC	1 cut(s)	705
SfaNI	GCATC	3 cut(s)	583, 1360, 1872
SfcI	CTRYAG	3 cut(s)	438, 867, 1902
SfoI	GGCGCC	1 cut(s)	156
SfuI	TTCGAA	4 cut(s)	724, 1519, 1630, 1648
SmaI	CCCGGG	1 cut(s)	909
SmiMI	CAYNNNNRTG	1 cut(s)	296
SmlI	CTYRAG	5 cut(s)	536, 1337, 1475, 1559, 1605
SmoI	CTYRAG	5 cut(s)	536, 1337, 1475, 1559, 1605
SphI	GCATGC	1 cut(s)	2205
SspDI	GGCGCC	1 cut(s)	154
StyD4I	CCNGG	5 cut(s)	132, 150, 588, 906, 907
StyI	CCWWGG	4 cut(s)	35, 753, 1038, 2116
TaaI	ACNGT	3 cut(s)	1111, 1533, 2160
TaiI	ACGT	5 cut(s)	424, 686, 794, 1887, 1951
TatI	WGTACW	2 cut(s)	1341, 1830
TauI	GCSGC	8 cut(s)	205, 220, 272, 278, 877, 886, 919, 1758
TfiI	GAWTC	4 cut(s)	478, 721, 766, 1555
Tru1I	TTAA	5 cut(s)	27, 953, 1254, 1803, 2172
Tru9I	TTAA	5 cut(s)	27, 953, 1254, 1803, 2172
TscAI	CASTG	5 cut(s)	129, 300, 447, 876, 2247
TseFI	GTSAC	4 cut(s)	1082, 1158, 2242, 2268
TseI	GCWGC	8 cut(s)	220, 272, 284, 437, 1471, 1543, 1758, 1895
Tsp45I	GTSAC	4 cut(s)	1082, 1158, 2242, 2268
TspGWI	ACGGA	3 cut(s)	59, 156, 2120
TspMI	CCCGGG	1 cut(s)	907
TspRI	CASTG	5 cut(s)	129, 300, 447, 876, 2247
Van91I	CCANNNNNTGG	1 cut(s)	1044
XagI	CCTNNNNNAGG	1 cut(s)	1854
XapI	RAATTY	4 cut(s)	1195, 1332, 2211, 2323
XbaI	TCTAGA	3 cut(s)	2216, 2254, 2342
XceI	RCATGY	1 cut(s)	2205
XcmI	CCANNNNNNNNNTGG	1 cut(s)	688
XmaI	CCCGGG	1 cut(s)	907
XmnI	GAANNNNTTC	4 cut(s)	563, 1239, 1554, 2350
Zsp2I	ATGCAT	1 cut(s)	1440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.