Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003211

Physical Location & Seq

Genomic Coordinates Forward (+)

63106768 .. 63109739

Gene Structure 2972 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG424800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGCCGGCGCTTTACATCTAATCATTATCCGGCCATGGCTTCTTCTTCTTCTTCTTCTGTTTTGTATTCTCAGTGGTAATTTTTTTGGTACTTGCTACGGTCAATCACAAAACATTGAGACTTTCTTTCCTTTTCCATTAACTCCAACCCCGGCGCCGTCTCCGATTATTCCGATCTCCCCTTCTTTACCTCCAGTAGAGTCCCCTCCGCCGGAGCCTCAACAGCCTCTGCCGCTGCCGCTGCCTCTGCCGGAAAAGAAGTCTAATGACAACAGCAACATTGCAAAGGCGGCAGCGGCAACCGCAGCCAGCACTCTGGTGGTGTGTGCGGTGGTCTTCTTTTTTGTGCAACGGTGCATTGTTGCTAAGCGGAGGAGGAAGATCAGCGGCGGTGTTATTGGTGCTGGTGCTAATAACAATTCCGGTTCACGAGGAAGCCGTCGTGGTCAACCGGCTGCAGTCAATGGCAATGAGTTTGAAAGGTACGATGGGAATCTCAAGGGGTTTATTGTGGACGAGGATGGTCTTGATGTGCTGTATTGGAGAAAACTCGAGGCCAAAAACTCGAAAAAGAAGAGTTTCAAGAAGGAGGCGGTGCGAATTCGCAATATTGGAGACGAAATTGATGAGTATGATGATGGAAATGGAGTAGGGACTAGCAGAGAGAATGAGCTTCCTCTGTTTCGAGGCAGGTCTTCGACGTCTGACATTGATTTAGTGCCGGAAGGAAATGATCGGGTTCGGATTGGAAGTAGTAGTAGAAGGATGGCTGCTAATAAGGGTGTTGAGATTAATCCAGAGCCAGTGGTGGAGCTGTCGCTTCAGTCTTCGGGTTCCTCGCCTCCACCTCATTCTCCACCACCAACACCACCTCCTCTGCCAATAAAAGCCCCTGCAGTGCCACTACCGCCGCCTCCTCCACCAGTTCCGGCTATGAAGGGCCCGGGACTGCCGCCTCCTCCACCACCAAAGCCTAAGTCAAGAAGTTTGAACGCCTTATCAAAACCAGAGCCAAAGGGGGTGAAGCTGAAGCCACTACATTGGGACAAGGTGAACACTAATCATGAGCATTCCATGGTGTGGGACAAAATTGATAAGGGTTCGTTCAGATTTGATGGAGACCTTATGGAAGCTCTTTTTGGAACTGTTGCCACTAATCGGAAATCTCCTGAAAGAGGCACTGGTTCATCGTCACAAATTTGCATCTTAGATGAGAGGAAGTCTCAGAACTTTGCAATTGTTATAAAATCATTGACCATTTCTATCGAAGATATTCTTGATGTGCTAAATGAGGGCAGAGGTTTAAGTGCTGAAAATCTCGAAAAGCTTGCTAGGATTGCCCCAACTGAAGAGGAAATAACCCAAATTCTTGCGTACAATGAAGACCCTAAAAGGCTTGCTGATGCTGAATGCTTCCTCTACCACATTTTGAAAGCTGTTCCATCAGCTTTTACTCGACTCAATGCCATGCATTTCAGACACAACTATGAGCAAGAGATTGCCCAACTCAAAGAGTCTTTACAGACAATTGAATTGGGATGCAAGGAACTTCGAACTAGAGGACTGTTTATGAAGCTGCTAGAAGCGATTCTCAAGGCTGGAAACCGAATGAATGCAGGAACTTCGAGAGGGAATGCGCAAGCTTTCAAGCTCAGTTCTCTTCGAAAACTATCTGATGTTCGAAGTAGTGATGGGAAGACTACTCTACTTCATTTTGTTGTGGAAGAAGTGGTGCGGTCTGAGGGAAAACGGGGTGTTTTGAACAGGAACCATAGCTTGAGCCGCAGCGGCAGCCAGAGCAGTAACGGCAACATGAGCTCTGAGCAGAATGTAAAATCGAAAGAGGATAGAGAGAAGGAGTATATGATGTTAGGATTGCCAGTGGTTGGAGGCATCAGTTCTGAGTTTTCAAATGTGAAGAAGGCAGCAACTATAGACTATGACAGCTTTGCAATCGCTTGCTCGGCTCTCACTACACGAGCTGCGGAAACTAGAGAACTAGTACTCCAATGTGAGAAGGATGGTGGTGGAAGATTTGTTTTTGAGATGAACGATTTTCTTGAAGATGCCGAAGAAGAGCTAAAGGCGATGGCAAAAGAGCAGAGTAGGGTGATGGAATTTGTGAAGACCACTACGGAATATTACCAAGGAGGAGCTTCAAAGGAAAAGGGATCACACCCATTTCAACTATTTGTTGTGGTTAAGGATTTTCTAGGCATGGTTGATCAAGCATGCGTGGAAATATCTCGAAATGTGCAGAGGAGGAAGGCAGTGACGGCAAGTTCAGGAACATCAACGTCACCAGACTCACCGCCATCAAGGAACCCTGTAAGGTTTCCAGTCTTACCAAAAAATTTCATGTCAGGAACCTCTAGAAGTAATTCTAGTGGATCAGATAATGAAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005522 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0010638 GO:0016020 GO:0016043 GO:0016049 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0033043 GO:0040007 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048522 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0060560 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2

Protein Analysis

802

Amino Acids

87.72

Weight (kDa)

8.89

Isoelectric Point (pI)

60.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	335 - 741	2.9e-101	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24150 AT1G59910 AT1G70140
fragaria_vesca	FvH4_4g35240 FvH4_5g16231
malus_domestica	MD06G1182500.v1.1 MD13G1085100.v1.1 MD14G1188500.v1.1 MD16G1084300.v1.1
prunus_persica	Prupe.1G261700_v2.0.a1 Prupe.5G180600_v2.0.a1
pyrus_communis	pycom06g16140 pycom13g07360
rosa_chinensis	RchiOBHm_Chr4g0444841 RchiOBHm_Chr7g0179861
rosa_laevigata	RLG00000005317 RLG00000005796
rosa_multiflora	Rmu_sc0000096.1_g000028 Rmu_sc0001754.1_g000001 Rmu_sc0006845.1_g000009 Rmu_sc0031375.1_g000001 Rmu_sc0034891.1_g000002 Rmu_sc0034892.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273390 Rroxscaffold_5G00385330
rosa_rugosa	Rorug04G0355500 Rorug06G0428800
rosa_samantha	Rh4AG417000 Rh4BG428800 Rh4CG443600 Rh4DG424800 Rh7AG028700 Rh7BG027900 Rh7CG029600 Rh7DG029000
rosa_wichuraiana	Rw4G035740 Rw7G002300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1244
AatII	GACGTC	1 cut(s)	704
Acc16I	TGCGCA	1 cut(s)	1638
Acc36I	ACCTGC	1 cut(s)	681
AccB1I	GGYRCC	1 cut(s)	154
AccB7I	CCANNNNNTGG	2 cut(s)	1080, 1886
AclWI	GGATC	2 cut(s)	2179, 2398
AcoI	YGGCCR	2 cut(s)	3, 32
AcsI	RAATTY	5 cut(s)	600, 1197, 1365, 2117, 2353
AcuI	CTGAAG	3 cut(s)	806, 1049, 1368
AcyI	GRCGYC	2 cut(s)	155, 701
AfaI	GTAC	4 cut(s)	91, 485, 1376, 2004
AfiI	CCNNNNNNNGG	5 cut(s)	211, 1080, 1158, 1175, 1886
AhlI	ACTAGT	1 cut(s)	1999
AjuI	GAANNNNNNNTTGG	2 cut(s)	1122, 1154
AleI	CACNNNNGTG	1 cut(s)	317
AloI	GAACNNNNNNTCC	2 cut(s)	1989, 2021
Alw21I	GWGCWC	1 cut(s)	1820
Alw26I	GTCTC	5 cut(s)	113, 165, 609, 1113, 1227
AlwI	GGATC	2 cut(s)	2179, 2398
AlwNI	CAGNNNCTG	1 cut(s)	229
Ama87I	CYCGRG	2 cut(s)	551, 943
AoxI	GGCC	4 cut(s)	3, 32, 555, 940
ApaI	GGGCCC	1 cut(s)	944
ApoI	RAATTY	5 cut(s)	600, 1197, 1365, 2117, 2353
ArsI	GACNNNNNNTTYG	2 cut(s)	962, 994
AseI	ATTAAT	1 cut(s)	792
Asp700I	GAANNNNTTC	4 cut(s)	578, 1272, 1587, 2380
AspLEI	GCGC	3 cut(s)	11, 157, 1639
AspS9I	GGNCC	2 cut(s)	940, 941
AsuC2I	CCSGG	3 cut(s)	152, 944, 945
AsuHPI	GGTGA	5 cut(s)	1033, 1063, 2122, 2292, 2301
AsuII	TTCGAA	3 cut(s)	1552, 1663, 1681
AvaI	CYCGRG	2 cut(s)	551, 943
BaeGI	GKGCMC	1 cut(s)	944
BanI	GGYRCC	1 cut(s)	154
BanII	GRGCYC	2 cut(s)	944, 1820
BauI	CACGAG	2 cut(s)	429, 1977
BbsI	GAAGAC	6 cut(s)	328, 687, 819, 1389, 1703, 2132
Bbv12I	GWGCWC	1 cut(s)	1820
BceAI	ACGGC	4 cut(s)	142, 423, 1822, 2292
BcgI	CGANNNNNNTGC	4 cut(s)	1310, 1344, 1605, 1639
BclI	TGATCA	1 cut(s)	2224
BcnI	CCSGG	3 cut(s)	152, 944, 945
BcoDI	GTCTC	5 cut(s)	113, 165, 609, 1113, 1227
BcuI	ACTAGT	1 cut(s)	1999
BfaI	CTAG	9 cut(s)	657, 1332, 1557, 1580, 1992, 2000, 2213, 2373, 2385
BfmI	CTRYAG	3 cut(s)	456, 894, 1932
BfoI	RGCGCY	2 cut(s)	12, 158
BfuAI	ACCTGC	1 cut(s)	681
BglI	GCCNNNNNGGC	1 cut(s)	1788
BlpI	GCTNAGC	1 cut(s)	366
BmcAI	AGTACT	1 cut(s)	2004
Bme1390I	CCNGG	3 cut(s)	152, 944, 945
BmeT110I	CYCGRG	2 cut(s)	551, 943
BmgT120I	GGNCC	2 cut(s)	940, 941
BmiI	GGNNCC	7 cut(s)	156, 216, 835, 942, 1769, 2324, 2368
BmrFI	CCNGG	3 cut(s)	152, 944, 945
BmsI	GCATC	5 cut(s)	1212, 1393, 1529, 1902, 2056
BpiI	GAAGAC	6 cut(s)	328, 687, 819, 1389, 1703, 2132
BplI	GAGNNNNNCTC	2 cut(s)	1207, 1239
BpmI	CTGGAG	1 cut(s)	177
Bpu1102I	GCTNAGC	1 cut(s)	366
Bpu14I	TTCGAA	3 cut(s)	1552, 1663, 1681
BpuEI	CTTGAG	3 cut(s)	482, 1577, 1798
BpuMI	CCSGG	3 cut(s)	152, 944, 945
BsaBI	GATNNNNATC	1 cut(s)	492
BsaHI	GRCGYC	2 cut(s)	155, 701
BsaI	GGTCTC	1 cut(s)	1113
BsaJI	CCNNGG	5 cut(s)	35, 150, 943, 1074, 2146
BsaWI	WCCGGW	1 cut(s)	422
BsaXI	ACNNNNNCTCC	4 cut(s)	856, 886, 1989, 2019
Bsc4I	CCNNNNNNNGG	5 cut(s)	211, 1080, 1158, 1175, 1886
Bse118I	RCCGGY	2 cut(s)	5, 451
Bse1I	ACTGG	6 cut(s)	194, 803, 923, 1186, 1880, 2339
Bse3DI	GCAATG	2 cut(s)	279, 475
Bse8I	GATNNNNATC	1 cut(s)	492
BseDI	CCNNGG	5 cut(s)	35, 150, 943, 1074, 2146
BseGI	GGATG	4 cut(s)	526, 771, 1544, 2026
BseJI	GATNNNNATC	1 cut(s)	492
BseLI	CCNNNNNNNGG	5 cut(s)	211, 1080, 1158, 1175, 1886
BseMI	GCAATG	2 cut(s)	279, 475
BseMII	CTCAG	6 cut(s)	85, 1238, 1666, 1731, 1812, 1893
BseNI	ACTGG	6 cut(s)	194, 803, 923, 1186, 1880, 2339
BseRI	GAGGAG	6 cut(s)	388, 864, 906, 948, 2166, 2275
BseSI	GKGCMC	1 cut(s)	944
BsgI	GTGCAG	1 cut(s)	2276
BshFI	GGCC	4 cut(s)	5, 34, 557, 942
BshNI	GGYRCC	1 cut(s)	154
BsiHKAI	GWGCWC	1 cut(s)	1820
BsiHKCI	CYCGRG	2 cut(s)	551, 943
BslFI	GGGAC	5 cut(s)	187, 667, 960, 1058, 1097
BslI	CCNNNNNNNGG	5 cut(s)	211, 1080, 1158, 1175, 1886
BsmAI	GTCTC	5 cut(s)	113, 165, 609, 1113, 1227
BsmBI	CGTCTC	2 cut(s)	165, 609
BsmFI	GGGAC	5 cut(s)	187, 667, 960, 1058, 1097
BsmI	GAATGC	4 cut(s)	1069, 1415, 1618, 1639
BsnI	GGCC	4 cut(s)	5, 34, 557, 942
Bso31I	GGTCTC	1 cut(s)	1113
BsoBI	CYCGRG	2 cut(s)	551, 943
Bsp119I	TTCGAA	3 cut(s)	1552, 1663, 1681
Bsp120I	GGGCCC	1 cut(s)	940
Bsp1286I	GDGCHC	2 cut(s)	944, 1820
Bsp143I	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
Bsp1720I	GCTNAGC	1 cut(s)	366
Bsp19I	CCATGG	2 cut(s)	35, 1074
BspANI	GGCC	4 cut(s)	5, 34, 557, 942
BspCNI	CTCAG	6 cut(s)	84, 1237, 1665, 1732, 1813, 1894
BspHI	TCATGA	1 cut(s)	1063
BspLI	GGNNCC	7 cut(s)	156, 216, 835, 942, 1769, 2324, 2368
BspMAI	CTGCAG	2 cut(s)	460, 898
BspMI	ACCTGC	1 cut(s)	681
BspPI	GGATC	2 cut(s)	2179, 2398
BspQI	GCTCTTC	1 cut(s)	2070
BspT104I	TTCGAA	3 cut(s)	1552, 1663, 1681
BspT107I	GGYRCC	1 cut(s)	154
BspTNI	GGTCTC	1 cut(s)	1113
BsrDI	GCAATG	2 cut(s)	279, 475
BsrFI	RCCGGY	2 cut(s)	5, 451
BsrI	ACTGG	6 cut(s)	194, 803, 923, 1186, 1880, 2339
BssAI	RCCGGY	2 cut(s)	5, 451
BssECI	CCNNGG	5 cut(s)	35, 150, 943, 1074, 2146
BssMI	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
BssNI	GRCGYC	2 cut(s)	155, 701
BssSI	CACGAG	2 cut(s)	429, 1977
BssT1I	CCWWGG	3 cut(s)	35, 1074, 2146
Bst2BI	CACGAG	2 cut(s)	429, 1977
Bst4CI	ACNGT	4 cut(s)	101, 354, 1147, 1566
Bst6I	CTCTTC	4 cut(s)	569, 1344, 1665, 2070
BstACI	GRCGYC	2 cut(s)	155, 701
BstBI	TTCGAA	3 cut(s)	1552, 1663, 1681
BstC8I	GCNNGC	7 cut(s)	7, 310, 1329, 1398, 1641, 1960, 2233
BstDEI	CTNAG	9 cut(s)	71, 366, 975, 1207, 1224, 1652, 1740, 1821, 1902
BstDSI	CCRYGG	2 cut(s)	35, 1074
BstENI	CCTNNNNNAGG	1 cut(s)	1173
BstF5I	GGATG	4 cut(s)	526, 771, 1544, 2026
BstH2I	RGCGCY	2 cut(s)	12, 158
BstHHI	GCGC	3 cut(s)	11, 157, 1639
BstKTI	GATC	6 cut(s)	177, 384, 736, 2174, 2227, 2393
BstMAI	GTCTC	5 cut(s)	113, 165, 609, 1113, 1227
BstMBI	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
BstNSI	RCATGY	1 cut(s)	2235
BstSCI	CCNGG	3 cut(s)	150, 942, 943
BstSFI	CTRYAG	3 cut(s)	456, 894, 1932
BstSLI	GKGCMC	1 cut(s)	944
BstV2I	GAAGAC	6 cut(s)	328, 687, 819, 1389, 1703, 2132
BstXI	CCANNNNNNTGG	2 cut(s)	316, 1041
BsuRI	GGCC	4 cut(s)	5, 34, 557, 942
BtgI	CCRYGG	2 cut(s)	35, 1074
BtgZI	GCGATG	1 cut(s)	2102
BtsCI	GGATG	4 cut(s)	526, 771, 1544, 2026
BtsI	GCAGTG	2 cut(s)	903, 2277
BtsIMutI	CAGTG	6 cut(s)	79, 810, 903, 1179, 1887, 2277
BveI	ACCTGC	1 cut(s)	681
Cac8I	GCNNGC	7 cut(s)	7, 310, 1329, 1398, 1641, 1960, 2233
CaiI	CAGNNNCTG	1 cut(s)	229
CciI	TCATGA	1 cut(s)	1063
CfoI	GCGC	3 cut(s)	11, 157, 1639
Cfr10I	RCCGGY	2 cut(s)	5, 451
Cfr13I	GGNCC	2 cut(s)	940, 941
Cfr9I	CCCGGG	1 cut(s)	943
Csp6I	GTAC	4 cut(s)	90, 484, 1375, 2003
CviAII	CATG	8 cut(s)	36, 1064, 1075, 1468, 1813, 2218, 2232, 2359
CviQI	GTAC	4 cut(s)	90, 484, 1375, 2003
DdeI	CTNAG	9 cut(s)	71, 366, 975, 1207, 1224, 1652, 1740, 1821, 1902
DinI	GGCGCC	1 cut(s)	156
DpnI	GATC	6 cut(s)	176, 383, 735, 2173, 2226, 2392
DpnII	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
EaeI	YGGCCR	2 cut(s)	3, 32
Eam1104I	CTCTTC	4 cut(s)	569, 1344, 1665, 2070
EarI	CTCTTC	4 cut(s)	569, 1344, 1665, 2070
EciI	GGCGGA	1 cut(s)	198
Ecl136II	GAGCTC	1 cut(s)	1818
Eco130I	CCWWGG	3 cut(s)	35, 1074, 2146
Eco24I	GRGCYC	2 cut(s)	944, 1820
Eco31I	GGTCTC	1 cut(s)	1113
Eco53kI	GAGCTC	1 cut(s)	1818
Eco57I	CTGAAG	3 cut(s)	806, 1049, 1368
Eco88I	CYCGRG	2 cut(s)	551, 943
EcoICRI	GAGCTC	1 cut(s)	1818
EcoNI	CCTNNNNNAGG	1 cut(s)	1173
EcoO109I	RGGNCCY	1 cut(s)	940
EcoRI	GAATTC	1 cut(s)	600
EcoT14I	CCWWGG	3 cut(s)	35, 1074, 2146
EcoT22I	ATGCAT	1 cut(s)	1473
EcoT38I	GRGCYC	2 cut(s)	944, 1820
EgeI	GGCGCC	1 cut(s)	156
EheI	GGCGCC	1 cut(s)	156
ErhI	CCWWGG	3 cut(s)	35, 1074, 2146
Esp3I	CGTCTC	2 cut(s)	165, 609
FaeI	CATG	8 cut(s)	39, 1067, 1078, 1471, 1816, 2221, 2235, 2362
FalI	AAGNNNNNCTT	2 cut(s)	1260, 1292
FaqI	GGGAC	5 cut(s)	187, 667, 960, 1058, 1097
FatI	CATG	8 cut(s)	35, 1063, 1074, 1467, 1812, 2217, 2231, 2358
FbaI	TGATCA	1 cut(s)	2224
FokI	GGATG	4 cut(s)	533, 778, 1551, 2033
FriOI	GRGCYC	2 cut(s)	944, 1820
FspBI	CTAG	9 cut(s)	657, 1332, 1557, 1580, 1992, 2000, 2213, 2373, 2385
FspI	TGCGCA	1 cut(s)	1638
GlaI	GCGC	3 cut(s)	10, 156, 1638
GsuI	CTGGAG	1 cut(s)	177
HaeII	RGCGCY	2 cut(s)	12, 158
HaeIII	GGCC	4 cut(s)	5, 34, 557, 942
HhaI	GCGC	3 cut(s)	11, 157, 1639
Hin1I	GRCGYC	2 cut(s)	155, 701
Hin1II	CATG	8 cut(s)	39, 1067, 1078, 1471, 1816, 2221, 2235, 2362
Hin6I	GCGC	3 cut(s)	9, 155, 1637
HinP1I	GCGC	3 cut(s)	9, 155, 1637
HincII	GTYRAC	1 cut(s)	449
HindII	GTYRAC	1 cut(s)	449
HindIII	AAGCTT	2 cut(s)	1325, 1641
HinfI	GANTC	6 cut(s)	200, 493, 1458, 1514, 1588, 2306
HphI	GGTGA	5 cut(s)	1033, 1063, 2122, 2292, 2301
Hpy166II	GTNNAC	4 cut(s)	428, 449, 514, 1054
Hpy8I	GTNNAC	4 cut(s)	428, 449, 514, 1054
Hpy99I	CGWCG	2 cut(s)	444, 703
HpyAV	CCTTC	9 cut(s)	192, 580, 719, 756, 931, 1849, 1915, 2011, 2260
HpyCH4III	ACNGT	4 cut(s)	101, 354, 1147, 1566
HpyCH4IV	ACGT	2 cut(s)	701, 2297
HpyF3I	CTNAG	9 cut(s)	71, 366, 975, 1207, 1224, 1652, 1740, 1821, 1902
HpySE526I	ACGT	2 cut(s)	701, 2297
Hsp92I	GRCGYC	2 cut(s)	155, 701
Hsp92II	CATG	8 cut(s)	39, 1067, 1078, 1471, 1816, 2221, 2235, 2362
HspAI	GCGC	3 cut(s)	9, 155, 1637
KasI	GGCGCC	1 cut(s)	154
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	2224
Kzo9I	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
LguI	GCTCTTC	1 cut(s)	2070
LmnI	GCTCC	3 cut(s)	214, 811, 2153
LweI	GCATC	5 cut(s)	1212, 1393, 1529, 1902, 2056
MaeI	CTAG	9 cut(s)	657, 1332, 1557, 1580, 1992, 2000, 2213, 2373, 2385
MaeII	ACGT	2 cut(s)	701, 2297
MaeIII	GTNAC	4 cut(s)	1191, 1802, 2272, 2298
MalI	GATC	6 cut(s)	176, 383, 735, 2173, 2226, 2392
MboI	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
MfeI	CAATTG	2 cut(s)	1236, 1527
MhlI	GDGCHC	2 cut(s)	944, 1820
Mly113I	GGCGCC	1 cut(s)	155
MlyI	GAGTC	4 cut(s)	209, 1452, 1523, 2300
MmeI	TCCRAC	2 cut(s)	170, 1867
Mph1103I	ATGCAT	1 cut(s)	1473
MroNI	GCCGGC	1 cut(s)	5
MroXI	GAANNNNTTC	4 cut(s)	578, 1272, 1587, 2380
MseI	TTAA	4 cut(s)	140, 792, 1304, 2202
MslI	CAYNNNNRTG	2 cut(s)	317, 1485
MspA1I	CMGCKG	5 cut(s)	235, 241, 296, 387, 1788
MspR9I	CCNGG	3 cut(s)	152, 944, 945
MunI	CAATTG	2 cut(s)	1236, 1527
Mva1269I	GAATGC	4 cut(s)	1069, 1415, 1618, 1639
NaeI	GCCGGC	1 cut(s)	7
NarI	GGCGCC	1 cut(s)	155
NciI	CCSGG	3 cut(s)	152, 944, 945
NcoI	CCATGG	2 cut(s)	35, 1074
NdeII	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	8 cut(s)	39, 1067, 1078, 1471, 1816, 2221, 2235, 2362
NlaIV	GGNNCC	7 cut(s)	156, 216, 835, 942, 1769, 2324, 2368
NmeAIII	GCCGAG	1 cut(s)	1943
NmuCI	GTSAC	3 cut(s)	1191, 2272, 2298
NsbI	TGCGCA	1 cut(s)	1638
NsiI	ATGCAT	1 cut(s)	1473
NspI	RCATGY	1 cut(s)	2235
NspV	TTCGAA	3 cut(s)	1552, 1663, 1681
OliI	CACNNNNGTG	1 cut(s)	317
PaeI	GCATGC	1 cut(s)	2235
PaeR7I	CTCGAG	1 cut(s)	551
PagI	TCATGA	1 cut(s)	1063
PciSI	GCTCTTC	1 cut(s)	2070
PcsI	WCGNNNNNNNCGW	1 cut(s)	436
PctI	GAATGC	4 cut(s)	1069, 1415, 1618, 1639
PdiI	GCCGGC	1 cut(s)	7
PdmI	GAANNNNTTC	4 cut(s)	578, 1272, 1587, 2380
PfeI	GAWTC	2 cut(s)	493, 1588
PflMI	CCANNNNNTGG	2 cut(s)	1080, 1886
PleI	GAGTC	4 cut(s)	208, 1452, 1522, 2300
PluTI	GGCGCC	1 cut(s)	158
PpsI	GAGTC	4 cut(s)	208, 1452, 1522, 2300
PshBI	ATTAAT	1 cut(s)	792
PsiI	TTATAA	1 cut(s)	1244
Psp124BI	GAGCTC	1 cut(s)	1820
PspN4I	GGNNCC	7 cut(s)	156, 216, 835, 942, 1769, 2324, 2368
PspOMI	GGGCCC	1 cut(s)	940
PspPI	GGNCC	2 cut(s)	940, 941
PspXI	VCTCGAGB	1 cut(s)	551
PstI	CTGCAG	2 cut(s)	460, 898
PstNI	CAGNNNCTG	1 cut(s)	229
RsaI	GTAC	4 cut(s)	91, 485, 1376, 2004
RsaNI	GTAC	4 cut(s)	90, 484, 1375, 2003
RseI	CAYNNNNRTG	2 cut(s)	317, 1485
SacI	GAGCTC	1 cut(s)	1820
SapI	GCTCTTC	1 cut(s)	2070
SaqAI	TTAA	4 cut(s)	140, 792, 1304, 2202
Sau3AI	GATC	6 cut(s)	174, 381, 733, 2171, 2224, 2390
Sau96I	GGNCC	2 cut(s)	940, 941
ScaI	AGTACT	1 cut(s)	2004
SchI	GAGTC	4 cut(s)	209, 1452, 1523, 2300
ScrFI	CCNGG	3 cut(s)	152, 944, 945
SduI	GDGCHC	2 cut(s)	944, 1820
SfaNI	GCATC	5 cut(s)	1212, 1393, 1529, 1902, 2056
SfcI	CTRYAG	3 cut(s)	456, 894, 1932
SfoI	GGCGCC	1 cut(s)	156
Sfr274I	CTCGAG	1 cut(s)	551
SfuI	TTCGAA	3 cut(s)	1552, 1663, 1681
SlaI	CTCGAG	1 cut(s)	551
SmaI	CCCGGG	1 cut(s)	945
SmiMI	CAYNNNNRTG	2 cut(s)	317, 1485
SmlI	CTYRAG	4 cut(s)	497, 551, 1592, 1777
SmoI	CTYRAG	4 cut(s)	497, 551, 1592, 1777
SpeI	ACTAGT	1 cut(s)	1999
SphI	GCATGC	1 cut(s)	2235
SspDI	GGCGCC	1 cut(s)	154
SspI	AATATT	2 cut(s)	610, 2141
SspMI	CTAG	9 cut(s)	657, 1332, 1557, 1580, 1992, 2000, 2213, 2373, 2385
SstI	GAGCTC	1 cut(s)	1820
StyD4I	CCNGG	3 cut(s)	150, 942, 943
StyI	CCWWGG	3 cut(s)	35, 1074, 2146
TaaI	ACNGT	4 cut(s)	101, 354, 1147, 1566
TaiI	ACGT	2 cut(s)	704, 2300
TatI	WGTACW	1 cut(s)	2002
TauI	GCSGC	9 cut(s)	235, 241, 293, 299, 390, 913, 955, 1785, 1791
TfiI	GAWTC	2 cut(s)	493, 1588
Tru1I	TTAA	4 cut(s)	140, 792, 1304, 2202
Tru9I	TTAA	4 cut(s)	140, 792, 1304, 2202
TscAI	CASTG	6 cut(s)	79, 810, 903, 1186, 1887, 2277
TseFI	GTSAC	3 cut(s)	1191, 2272, 2298
Tsp45I	GTSAC	3 cut(s)	1191, 2272, 2298
TspDTI	ATGAA	8 cut(s)	950, 1176, 1395, 1586, 1625, 1700, 2063, 2347
TspGWI	ACGGA	1 cut(s)	2150
TspMI	CCCGGG	1 cut(s)	943
TspRI	CASTG	6 cut(s)	79, 810, 903, 1186, 1887, 2277
Van91I	CCANNNNNTGG	2 cut(s)	1080, 1886
VspI	ATTAAT	1 cut(s)	792
XagI	CCTNNNNNAGG	1 cut(s)	1173
XapI	RAATTY	5 cut(s)	600, 1197, 1365, 2117, 2353
XbaI	TCTAGA	1 cut(s)	2372
XceI	RCATGY	1 cut(s)	2235
XcmI	CCANNNNNNNNNTGG	1 cut(s)	316
XhoI	CTCGAG	1 cut(s)	551
XmaI	CCCGGG	1 cut(s)	943
XmnI	GAANNNNTTC	4 cut(s)	578, 1272, 1587, 2380
XspI	CTAG	9 cut(s)	657, 1332, 1557, 1580, 1992, 2000, 2213, 2373, 2385
ZraI	GACGTC	1 cut(s)	702
ZrmI	AGTACT	1 cut(s)	2004
Zsp2I	ATGCAT	1 cut(s)	1473

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.