Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003211

Physical Location & Seq

Genomic Coordinates Forward (+)

1934162 .. 1936505

Gene Structure 2344 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG027900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGACAACCTCAAATGGAATAATGCTGGCCAAGATCATTGCAGCCACGGCTGCAACTACTCTTGTTATTGCCGGAATCTTCTTCTTCGTCTACAGATTTCTAGCTGCTCGGCGCCACCCAAAAGACAAGAGCAATGGAAGCTTTCGTAGGGAGGAAGTTAGAATGAGTCGTGAAGATCAGCTCAGGCAATGCGGTGGAAATGTGAAGGGATTGATTGTCGATGAGAATGGTGTGGATGTTATCTACTTGAGGAAACTAGAAGTTGGCCAGGTTGAAACTCATTTTCCCAAGGTAGTTTTCAACCCAAGTTATGAAGACGATGTAGAAGAAGAAGAAAAGAGAGTGGATGCTAAAGGAGAAAGAGCCAAAATGCCCAAGCCTAAAGCGGTTCGTTCTTTTCATAAGTCATCTGATTTGATCAATCATGGAAAATTAGCAAAACCATTCATACAAAATCCAACTCCAGAAGTTGTGCTTTCATTGCCAAAGCCGCAACAGCGGCTTCCTCTAAAATCTCCTGCACCTCCATTTAGGATAACACCACCAAAGATGTTGGAAAAGCAAGCACTAGAATCACTGCCTCCTCTACCACCCCCACCATCAAGTAATATATCTCCACCACCATCAGTTCCTCCACCACCACCTCCTCCACCACCACCCCCGCCACCACAGCTACCTAAGATAGTAAGAAGAAAATCTATACCACCCACTCCACCTAGGCTAGGTGGTTCTTTTTTAGCGCCAAAACCACCACCTACACCTAAAGGCATACCAAACAACAAAAGTAGGGCTGAGGCCTCAGCTGGGGAGAGCTCAAAAGTGAGTGTCATTAGGCAAACGAAGCTGAAGCCATTGCACTGGGACAAGGTTATGGCTGCTGTTGATCATTCCATGGTCTGGGATCAAATCACTGATGGAACATTCAGATTTGATGATGAGCTTATGGAAAACTTGTTTGGATATAACATCACCAAAAACCAAACCAATGAAAGAAATAACAATTTGTCAGCTTCAGCCAAGTCTAACTCTGCCCCAACAGCTCAAGTTTTCATTCTAGACCCCAGAAAGTCTCAAAACACAGCAATAGTACTAAAGTCTCTCCCAATCTCTCGTAAAGAAATCATAAATTCTCTTCTTGATGGTCAAGGACTCGGTTCAGAAACACTCGAAAAACTCACCAAAATTTCTCCAACCAAAGAAGAAGAGATCAAGATCCTCCAATTCAATGGCAATCCAAATAAGCTTGCAGATGCTGAGTCTTTCCTCTACCACATCCTCAAAGCTGTCCCTTCGGCATTTACACGATTCAATGCAATGCTTTTCCGGGAAAATTACGACACCGAAATCCTCCACCTCAAGGAGTCACTACAAACACTTGAACTTGGGTGCAAGGAGCTAAGAGCTCGAGGTTTTTTCCTTAAACTTCTTGAAGCGATTCTCAAAGCTGGAAACCAAATGAATGCTGGAACTCCTCGAGGAAATGCACAAGGTTTCAACCTTAGTGCTCTTCTGAATCTATCTGATGTCAAAAGCACTGATGCAAAGACCACTCTGCTTCACTTTGTGGTTGAACAAGTAGCTCAATCGGAGGGTAGAGACTTAGTGATCAATCAGAGAAGCCAAACTAGTACACCAAATTCGGATAATCTGACAGCCGAAGAGAGAAGAAAGGAGTACTTAATCCAAGGGTTACCAAAGTTGGGAGGCTTGAGTATTGAGCTATCTAATGTGAAGAAAGCAGCTACAATAGACTATGACAGCTTCATTCGTGTGAGCTCCACTCTCACTAGCCGTGTAGATGAAATTAGAAAGGTTTTGACACATTGTTGCAATGGTGAGACAGGAGGGTTTGCAAGAGAAATGAAAGGGTTTTTGGAGGAATGTGAGGAGCTTAAGGTTGTGAGAGAAGAGCAGATTAGGGTTATGGAACTTGTGAAGAGAACGACGGAGTATTACCAAGCCAGATCTGCGAAAGAAAAAGGATCTCAACCACTTCAGCTCTTTGTGATTGTTAGAGGTTTTCTAGACATGGTTGATCTTGTTTGTGCAGAAGTTTCAAGAAAGGTACAGAGGAAGAGTACTTCTACAACCAATGTGGGATCAGCATCTCCTCCATTGTCACCTACAGTCCTAACACCAAAGAAGTTACAGAACTTCCAGTCACATTTCAATTCACATGTGTCTTGGTCATCTTCCAGTGATTCAGAAGATGATTTCAGAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005522 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0010638 GO:0016020 GO:0016043 GO:0016049 GO:0030029 GO:0030036 GO:0030054 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0033043 GO:0040007 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048522 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0060560 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2

Protein Analysis

741

Amino Acids

82.35

Weight (kDa)

9.4

Isoelectric Point (pI)

47.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	278 - 678	1.9e-96	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24150 AT1G59910 AT1G70140
fragaria_vesca	FvH4_4g35240 FvH4_5g16231
malus_domestica	MD06G1182500.v1.1 MD13G1085100.v1.1 MD14G1188500.v1.1 MD16G1084300.v1.1
prunus_persica	Prupe.1G261700_v2.0.a1 Prupe.5G180600_v2.0.a1
pyrus_communis	pycom06g16140 pycom13g07360
rosa_chinensis	RchiOBHm_Chr4g0444841 RchiOBHm_Chr7g0179861
rosa_laevigata	RLG00000005317 RLG00000005796
rosa_multiflora	Rmu_sc0000096.1_g000028 Rmu_sc0001754.1_g000001 Rmu_sc0006845.1_g000009 Rmu_sc0031375.1_g000001 Rmu_sc0034891.1_g000002 Rmu_sc0034892.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273390 Rroxscaffold_5G00385330
rosa_rugosa	Rorug04G0355500 Rorug06G0428800
rosa_samantha	Rh4AG417000 Rh4BG428800 Rh4CG443600 Rh4DG424800 Rh7AG028700 Rh7BG027900 Rh7CG029600 Rh7DG029000
rosa_wichuraiana	Rw4G035740 Rw7G002300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1473
AccB1I	GGYRCC	1 cut(s)	111
AccB7I	CCANNNNNTGG	1 cut(s)	897
AccI	GTMKAC	1 cut(s)	90
AciI	CCGC	5 cut(s)	192, 386, 491, 499, 662
AclWI	GGATC	4 cut(s)	909, 1207, 1990, 2107
AcoI	YGGCCR	2 cut(s)	27, 265
AcsI	RAATTY	3 cut(s)	1126, 1182, 1636
AcuI	CTGAAG	3 cut(s)	866, 996, 1979
AcyI	GRCGYC	1 cut(s)	112
AdeI	CACNNNGTG	1 cut(s)	1564
AfaI	GTAC	5 cut(s)	1089, 1630, 1676, 2067, 2080
AfiI	CCNNNNNNNGG	5 cut(s)	722, 804, 897, 1357, 1700
AflII	CTTAAG	1 cut(s)	1892
AflIII	ACRYGT	1 cut(s)	2176
AhlI	ACTAGT	1 cut(s)	1625
AjnI	CCWGG	1 cut(s)	267
AjuI	GAANNNNNNNTTGG	6 cut(s)	281, 313, 939, 971, 1856, 1888
Alw21I	GWGCWC	4 cut(s)	815, 1405, 1507, 1778
Alw26I	GTCTC	4 cut(s)	1074, 1101, 1590, 1832
AlwI	GGATC	4 cut(s)	909, 1207, 1990, 2107
AlwNI	CAGNNNCTG	1 cut(s)	1253
Ama87I	CYCGRG	2 cut(s)	1404, 1473
AoxI	GGCC	3 cut(s)	27, 265, 795
ApeKI	GCWGC	5 cut(s)	41, 50, 104, 875, 1739
ApoI	RAATTY	3 cut(s)	1126, 1182, 1636
Asp700I	GAANNNNTTC	1 cut(s)	1434
AspA2I	CCTAGG	1 cut(s)	716
AspLEI	GCGC	2 cut(s)	114, 742
AsuC2I	CCSGG	1 cut(s)	1325
AsuHPI	GGTGA	4 cut(s)	961, 1168, 1847, 2112
AvaI	CYCGRG	2 cut(s)	1404, 1473
AvrII	CCTAGG	1 cut(s)	716
BalI	TGGCCA	2 cut(s)	29, 267
BanI	GGYRCC	1 cut(s)	111
BanII	GRGCYC	3 cut(s)	815, 1405, 1778
BbsI	GAAGAC	1 cut(s)	321
Bbv12I	GWGCWC	4 cut(s)	815, 1405, 1507, 1778
BbvCI	CCTCAGC	2 cut(s)	792, 799
BbvI	GCAGC	5 cut(s)	37, 53, 91, 862, 1751
BccI	CCATC	4 cut(s)	607, 631, 908, 1133
BceAI	ACGGC	2 cut(s)	63, 1776
BciT130I	CCWGG	1 cut(s)	269
BclI	TGATCA	3 cut(s)	417, 883, 1605
BcnI	CCSGG	1 cut(s)	1325
BcoDI	GTCTC	4 cut(s)	1074, 1101, 1590, 1832
BcuI	ACTAGT	1 cut(s)	1625
BfaI	CTAG	9 cut(s)	101, 257, 569, 717, 722, 1055, 1626, 1788, 2024
BfmI	CTRYAG	2 cut(s)	91, 2124
BfoI	RGCGCY	2 cut(s)	115, 743
BfrI	CTTAAG	1 cut(s)	1892
BglII	AGATCT	1 cut(s)	1964
BisI	GCNGC	7 cut(s)	42, 51, 105, 491, 500, 876, 1740
BlnI	CCTAGG	1 cut(s)	716
BlsI	GCNGC	7 cut(s)	43, 52, 106, 492, 501, 877, 1741
BmcAI	AGTACT	3 cut(s)	1089, 1676, 2080
Bme1390I	CCNGG	2 cut(s)	269, 1325
BmeT110I	CYCGRG	2 cut(s)	1404, 1473
BmiI	GGNNCC	1 cut(s)	113
BmrFI	CCNGG	2 cut(s)	269, 1325
BmrI	ACTGGG	1 cut(s)	868
BmsI	GCATC	4 cut(s)	337, 1240, 1528, 2114
BmuI	ACTGGG	1 cut(s)	868
BpiI	GAAGAC	1 cut(s)	321
BplI	GAGNNNNNCTC	2 cut(s)	1765, 1797
BpmI	CTGGAG	1 cut(s)	447
Bpu10I	CCTNAGC	3 cut(s)	182, 792, 799
BpuEI	CTTGAG	4 cut(s)	268, 1026, 1340, 1729
BpuMI	CCSGG	1 cut(s)	1325
BsaBI	GATNNNNATC	2 cut(s)	1211, 2104
BsaHI	GRCGYC	1 cut(s)	112
BsaJI	CCNNGG	5 cut(s)	45, 288, 716, 891, 1684
Bsc4I	CCNNNNNNNGG	5 cut(s)	722, 804, 897, 1357, 1700
Bse1I	ACTGG	3 cut(s)	863, 2158, 2196
Bse3DI	GCAATG	7 cut(s)	36, 139, 194, 479, 851, 1320, 1837
Bse8I	GATNNNNATC	2 cut(s)	1211, 2104
BseBI	CCWGG	1 cut(s)	269
BseDI	CCNNGG	5 cut(s)	45, 288, 716, 891, 1684
BseGI	GGATG	3 cut(s)	241, 352, 1272
BseJI	GATNNNNATC	2 cut(s)	1211, 2104
BseLI	CCNNNNNNNGG	5 cut(s)	722, 804, 897, 1357, 1700
BseMI	GCAATG	7 cut(s)	36, 139, 194, 479, 851, 1320, 1837
BseMII	CTCAG	4 cut(s)	196, 783, 813, 1245
BseNI	ACTGG	3 cut(s)	863, 2158, 2196
BseRI	GAGGAG	5 cut(s)	573, 636, 1461, 1901, 2100
BseXI	GCAGC	5 cut(s)	37, 53, 91, 862, 1751
BseYI	CCCAGC	1 cut(s)	803
BsgI	GTGCAG	2 cut(s)	504, 2067
BshFI	GGCC	3 cut(s)	29, 267, 797
BshNI	GGYRCC	1 cut(s)	111
BsiHKAI	GWGCWC	4 cut(s)	815, 1405, 1507, 1778
BsiHKCI	CYCGRG	2 cut(s)	1404, 1473
BsiSI	CCGG	2 cut(s)	72, 1324
BslFI	GGGAC	2 cut(s)	875, 1271
BslI	CCNNNNNNNGG	5 cut(s)	722, 804, 897, 1357, 1700
BsmAI	GTCTC	4 cut(s)	1074, 1101, 1590, 1832
BsmFI	GGGAC	2 cut(s)	875, 1271
BsmI	GAATGC	1 cut(s)	1465
BsnI	GGCC	3 cut(s)	29, 267, 797
BsoBI	CYCGRG	2 cut(s)	1404, 1473
Bsp1286I	GDGCHC	4 cut(s)	815, 1405, 1507, 1778
Bsp19I	CCATGG	1 cut(s)	891
BspACI	CCGC	5 cut(s)	192, 386, 491, 499, 662
BspANI	GGCC	3 cut(s)	29, 267, 797
BspCNI	CTCAG	4 cut(s)	195, 784, 812, 1246
BspLI	GGNNCC	1 cut(s)	113
BspPI	GGATC	4 cut(s)	909, 1207, 1990, 2107
BspQI	GCTCTTC	2 cut(s)	1512, 1902
BspT107I	GGYRCC	1 cut(s)	111
BspTI	CTTAAG	1 cut(s)	1892
BsrDI	GCAATG	7 cut(s)	36, 139, 194, 479, 851, 1320, 1837
BsrI	ACTGG	3 cut(s)	863, 2158, 2196
BssECI	CCNNGG	5 cut(s)	45, 288, 716, 891, 1684
BssNI	GRCGYC	1 cut(s)	112
BssT1I	CCWWGG	4 cut(s)	288, 716, 891, 1684
Bst2UI	CCWGG	1 cut(s)	269
Bst4CI	ACNGT	1 cut(s)	2128
Bst6I	CTCTTC	7 cut(s)	1137, 1197, 1512, 1653, 1902, 1931, 2069
BstACI	GRCGYC	1 cut(s)	112
BstAFI	CTTAAG	1 cut(s)	1892
BstC8I	GCNNGC	3 cut(s)	27, 564, 1245
BstDEI	CTNAG	8 cut(s)	182, 678, 792, 799, 1254, 1397, 1499, 1600
BstDSI	CCRYGG	2 cut(s)	45, 891
BstEII	GGTNACC	1 cut(s)	1689
BstENI	CCTNNNNNAGG	1 cut(s)	720
BstF5I	GGATG	3 cut(s)	241, 352, 1272
BstH2I	RGCGCY	2 cut(s)	115, 743
BstHHI	GCGC	2 cut(s)	114, 742
BstMAI	GTCTC	4 cut(s)	1074, 1101, 1590, 1832
BstNI	CCWGG	1 cut(s)	269
BstNSI	RCATGY	1 cut(s)	2180
BstPI	GGTNACC	1 cut(s)	1689
BstSCI	CCNGG	2 cut(s)	267, 1323
BstSFI	CTRYAG	2 cut(s)	91, 2124
BstV1I	GCAGC	5 cut(s)	37, 53, 91, 862, 1751
BstV2I	GAAGAC	1 cut(s)	321
BstX2I	RGATCY	3 cut(s)	1212, 1964, 1982
BstXI	CCANNNNNNTGG	2 cut(s)	858, 1226
BstYI	RGATCY	3 cut(s)	1212, 1964, 1982
BsuRI	GGCC	3 cut(s)	29, 267, 797
BtgI	CCRYGG	2 cut(s)	45, 891
BtsCI	GGATG	3 cut(s)	241, 352, 1272
BtsI	GCAGTG	1 cut(s)	575
BtsIMutI	CAGTG	5 cut(s)	575, 856, 909, 1533, 2203
Cac8I	GCNNGC	3 cut(s)	27, 564, 1245
CaiI	CAGNNNCTG	1 cut(s)	1253
CfoI	GCGC	2 cut(s)	114, 742
Csp6I	GTAC	5 cut(s)	1088, 1629, 1675, 2066, 2079
CspCI	CAANNNNNGTGG	2 cut(s)	2076, 2111
CviAII	CATG	4 cut(s)	425, 892, 2029, 2177
CviQI	GTAC	5 cut(s)	1088, 1629, 1675, 2066, 2079
DdeI	CTNAG	8 cut(s)	182, 678, 792, 799, 1254, 1397, 1499, 1600
DinI	GGCGCC	1 cut(s)	113
DraIII	CACNNNGTG	1 cut(s)	1564
EaeI	YGGCCR	2 cut(s)	27, 265
Eam1104I	CTCTTC	7 cut(s)	1137, 1197, 1512, 1653, 1902, 1931, 2069
EarI	CTCTTC	7 cut(s)	1137, 1197, 1512, 1653, 1902, 1931, 2069
Ecl136II	GAGCTC	3 cut(s)	813, 1403, 1776
Eco130I	CCWWGG	4 cut(s)	288, 716, 891, 1684
Eco147I	AGGCCT	1 cut(s)	797
Eco24I	GRGCYC	3 cut(s)	815, 1405, 1778
Eco53kI	GAGCTC	3 cut(s)	813, 1403, 1776
Eco57I	CTGAAG	3 cut(s)	866, 996, 1979
Eco88I	CYCGRG	2 cut(s)	1404, 1473
Eco91I	GGTNACC	1 cut(s)	1689
EcoICRI	GAGCTC	3 cut(s)	813, 1403, 1776
EcoNI	CCTNNNNNAGG	1 cut(s)	720
EcoO65I	GGTNACC	1 cut(s)	1689
EcoRII	CCWGG	1 cut(s)	267
EcoT14I	CCWWGG	4 cut(s)	288, 716, 891, 1684
EcoT38I	GRGCYC	3 cut(s)	815, 1405, 1778
EgeI	GGCGCC	1 cut(s)	113
EheI	GGCGCC	1 cut(s)	113
ErhI	CCWWGG	4 cut(s)	288, 716, 891, 1684
FaeI	CATG	4 cut(s)	428, 895, 2032, 2180
FalI	AAGNNNNNCTT	4 cut(s)	459, 491, 1661, 1693
FaqI	GGGAC	2 cut(s)	875, 1271
FatI	CATG	4 cut(s)	424, 891, 2028, 2176
FauI	CCCGC	1 cut(s)	669
FbaI	TGATCA	3 cut(s)	417, 883, 1605
FblI	GTMKAC	1 cut(s)	90
Fnu4HI	GCNGC	7 cut(s)	42, 51, 105, 491, 500, 876, 1740
FokI	GGATG	3 cut(s)	248, 359, 1259
FriOI	GRGCYC	3 cut(s)	815, 1405, 1778
Fsp4HI	GCNGC	7 cut(s)	42, 51, 105, 491, 500, 876, 1740
FspBI	CTAG	9 cut(s)	101, 257, 569, 717, 722, 1055, 1626, 1788, 2024
GlaI	GCGC	2 cut(s)	113, 741
GluI	GCNGC	7 cut(s)	42, 51, 105, 491, 500, 876, 1740
GsaI	CCCAGC	1 cut(s)	807
GsuI	CTGGAG	1 cut(s)	447
HaeII	RGCGCY	2 cut(s)	115, 743
HaeIII	GGCC	3 cut(s)	29, 267, 797
HapII	CCGG	2 cut(s)	72, 1324
HhaI	GCGC	2 cut(s)	114, 742
Hin1I	GRCGYC	1 cut(s)	112
Hin1II	CATG	4 cut(s)	428, 895, 2032, 2180
Hin6I	GCGC	2 cut(s)	112, 740
HinP1I	GCGC	2 cut(s)	112, 740
HindIII	AAGCTT	2 cut(s)	139, 1241
HpaII	CCGG	2 cut(s)	72, 1324
HphI	GGTGA	4 cut(s)	961, 1168, 1847, 2112
Hpy166II	GTNNAC	2 cut(s)	91, 1631
Hpy188III	TCNNGA	8 cut(s)	170, 464, 1055, 1136, 1210, 1427, 2024, 2058
Hpy8I	GTNNAC	2 cut(s)	91, 1631
Hpy99I	CGWCG	1 cut(s)	1948
HpyAV	CCTTC	2 cut(s)	199, 1299
HpyCH4III	ACNGT	1 cut(s)	2128
HpyF3I	CTNAG	8 cut(s)	182, 678, 792, 799, 1254, 1397, 1499, 1600
Hsp92I	GRCGYC	1 cut(s)	112
Hsp92II	CATG	4 cut(s)	428, 895, 2032, 2180
HspAI	GCGC	2 cut(s)	112, 740
KasI	GGCGCC	1 cut(s)	111
Ksp22I	TGATCA	3 cut(s)	417, 883, 1605
LguI	GCTCTTC	2 cut(s)	1512, 1902
LmnI	GCTCC	3 cut(s)	1393, 1781, 1888
Lsp1109I	GCAGC	5 cut(s)	37, 53, 91, 862, 1751
LweI	GCATC	4 cut(s)	337, 1240, 1528, 2114
MaeI	CTAG	9 cut(s)	101, 257, 569, 717, 722, 1055, 1626, 1788, 2024
MaeIII	GTNAC	5 cut(s)	1362, 1689, 2118, 2145, 2160
MflI	RGATCY	3 cut(s)	1212, 1964, 1982
MhlI	GDGCHC	4 cut(s)	815, 1405, 1507, 1778
MlsI	TGGCCA	2 cut(s)	29, 267
MluCI	AATT	9 cut(s)	431, 1000, 1126, 1182, 1220, 1330, 1636, 1803, 2170
MluNI	TGGCCA	2 cut(s)	29, 267
Mly113I	GGCGCC	1 cut(s)	112
MlyI	GAGTC	4 cut(s)	175, 1143, 1265, 1370
MmeI	TCCRAC	3 cut(s)	482, 534, 1214
Mox20I	TGGCCA	2 cut(s)	29, 267
MroXI	GAANNNNTTC	1 cut(s)	1434
MscI	TGGCCA	2 cut(s)	29, 267
MseI	TTAA	3 cut(s)	1419, 1679, 1893
MslI	CAYNNNNRTG	1 cut(s)	1769
Msp20I	TGGCCA	2 cut(s)	29, 267
MspA1I	CMGCKG	2 cut(s)	499, 803
MspCI	CTTAAG	1 cut(s)	1892
MspI	CCGG	2 cut(s)	72, 1324
MspR9I	CCNGG	2 cut(s)	269, 1325
Mva1269I	GAATGC	1 cut(s)	1465
MvaI	CCWGG	1 cut(s)	269
NarI	GGCGCC	1 cut(s)	112
NciI	CCSGG	1 cut(s)	1325
NcoI	CCATGG	1 cut(s)	891
NlaIII	CATG	4 cut(s)	428, 895, 2032, 2180
NlaIV	GGNNCC	1 cut(s)	113
NmeAIII	GCCGAG	1 cut(s)	88
NmuCI	GTSAC	3 cut(s)	1362, 2118, 2160
NspI	RCATGY	1 cut(s)	2180
PaeR7I	CTCGAG	2 cut(s)	1404, 1473
PceI	AGGCCT	1 cut(s)	797
PciI	ACATGT	1 cut(s)	2176
PciSI	GCTCTTC	2 cut(s)	1512, 1902
PctI	GAATGC	1 cut(s)	1465
PdmI	GAANNNNTTC	1 cut(s)	1434
PfeI	GAWTC	6 cut(s)	75, 572, 1305, 1435, 1513, 2201
PflMI	CCANNNNNTGG	1 cut(s)	897
PfoI	TCCNGGA	1 cut(s)	1323
PkrI	GCNGC	7 cut(s)	43, 52, 106, 492, 501, 877, 1741
PleI	GAGTC	4 cut(s)	174, 1143, 1264, 1369
PluTI	GGCGCC	1 cut(s)	115
PpsI	GAGTC	4 cut(s)	174, 1143, 1264, 1369
PscI	ACATGT	1 cut(s)	2176
Psp124BI	GAGCTC	3 cut(s)	815, 1405, 1778
Psp6I	CCWGG	1 cut(s)	267
PspEI	GGTNACC	1 cut(s)	1689
PspFI	CCCAGC	1 cut(s)	803
PspGI	CCWGG	1 cut(s)	267
PspN4I	GGNNCC	1 cut(s)	113
PspXI	VCTCGAGB	2 cut(s)	1404, 1473
PstNI	CAGNNNCTG	1 cut(s)	1253
PsuI	RGATCY	3 cut(s)	1212, 1964, 1982
PvuII	CAGCTG	1 cut(s)	803
RsaI	GTAC	5 cut(s)	1089, 1630, 1676, 2067, 2080
RsaNI	GTAC	5 cut(s)	1088, 1629, 1675, 2066, 2079
RseI	CAYNNNNRTG	1 cut(s)	1769
SacI	GAGCTC	3 cut(s)	815, 1405, 1778
SapI	GCTCTTC	2 cut(s)	1512, 1902
SaqAI	TTAA	3 cut(s)	1419, 1679, 1893
SatI	GCNGC	7 cut(s)	42, 51, 105, 491, 500, 876, 1740
ScaI	AGTACT	3 cut(s)	1089, 1676, 2080
SchI	GAGTC	4 cut(s)	175, 1143, 1265, 1370
ScrFI	CCNGG	2 cut(s)	269, 1325
SduI	GDGCHC	4 cut(s)	815, 1405, 1507, 1778
SfaNI	GCATC	4 cut(s)	337, 1240, 1528, 2114
SfcI	CTRYAG	2 cut(s)	91, 2124
SfoI	GGCGCC	1 cut(s)	113
Sfr274I	CTCGAG	2 cut(s)	1404, 1473
SlaI	CTCGAG	2 cut(s)	1404, 1473
SmiMI	CAYNNNNRTG	1 cut(s)	1769
SmlI	CTYRAG	7 cut(s)	247, 1041, 1355, 1404, 1473, 1708, 1892
SmoI	CTYRAG	7 cut(s)	247, 1041, 1355, 1404, 1473, 1708, 1892
SpeI	ACTAGT	1 cut(s)	1625
Sse9I	AATT	9 cut(s)	431, 1000, 1126, 1182, 1220, 1330, 1636, 1803, 2170
SseBI	AGGCCT	1 cut(s)	797
SsiI	CCGC	5 cut(s)	192, 386, 491, 499, 662
SspDI	GGCGCC	1 cut(s)	111
SspMI	CTAG	9 cut(s)	101, 257, 569, 717, 722, 1055, 1626, 1788, 2024
SstI	GAGCTC	3 cut(s)	815, 1405, 1778
StuI	AGGCCT	1 cut(s)	797
StyD4I	CCNGG	2 cut(s)	267, 1323
StyI	CCWWGG	4 cut(s)	288, 716, 891, 1684
TaaI	ACNGT	1 cut(s)	2128
TaqI	TCGA	4 cut(s)	219, 1167, 1405, 1474
TasI	AATT	9 cut(s)	431, 1000, 1126, 1182, 1220, 1330, 1636, 1803, 2170
TatI	WGTACW	4 cut(s)	1087, 1628, 1674, 2078
TauI	GCSGC	2 cut(s)	493, 502
TfiI	GAWTC	6 cut(s)	75, 572, 1305, 1435, 1513, 2201
Tru1I	TTAA	3 cut(s)	1419, 1679, 1893
Tru9I	TTAA	3 cut(s)	1419, 1679, 1893
TscAI	CASTG	5 cut(s)	582, 863, 916, 1540, 2203
TseFI	GTSAC	3 cut(s)	1362, 2118, 2160
TseI	GCWGC	5 cut(s)	41, 50, 104, 875, 1739
Tsp45I	GTSAC	3 cut(s)	1362, 2118, 2160
TspGWI	ACGGA	1 cut(s)	1961
TspRI	CASTG	5 cut(s)	582, 863, 916, 1540, 2203
Van91I	CCANNNNNTGG	1 cut(s)	897
Vha464I	CTTAAG	1 cut(s)	1892
XagI	CCTNNNNNAGG	1 cut(s)	720
XapI	RAATTY	3 cut(s)	1126, 1182, 1636
XbaI	TCTAGA	2 cut(s)	1054, 2023
XceI	RCATGY	1 cut(s)	2180
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1460
XhoI	CTCGAG	2 cut(s)	1404, 1473
XmaJI	CCTAGG	1 cut(s)	716
XmiI	GTMKAC	1 cut(s)	90
XmnI	GAANNNNTTC	1 cut(s)	1434
XspI	CTAG	9 cut(s)	101, 257, 569, 717, 722, 1055, 1626, 1788, 2024
ZrmI	AGTACT	3 cut(s)	1089, 1676, 2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.