FvH4_5g02310

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004720
Physical Location & Seq
Reverse (-)
1408579 .. 1421236
12658 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02310.t3

Sequence Viewer

Length: 5517 bp
ATGGGTAAAGGAAGACCTAGGGCTGTGGAGAAGGGGGTGGTGGGCCCCAATTTGAGCTGTGCTTCGTCTTCTTCGTTGACCATACCGTCTGCGCCGGTGTATTATCCGAGTGAGGATGAGTTTAGGGACCCTTTGGAGTATATATGCAAGATCAGGGCTGAGGCTGAGCCGTACGGGATTTGTAGGATAGTTCCGCCGGAGAGCTGGAAGCCTCCTTTTGCTTTGGACTTGGAGAAGTTCACGTTTCCGACAAAGACGCAGGCGATTCATCAGTTGCAGGTGAGGCCTGCTTCGTGTGATTCCAAGACTTTTGAGCTGGAGTATAATAGGTTTTTGGAGGATCATTGCGGGAAGAGGCTGAGGAGGAAGGTGGTGTTTGAGGGGGAGGAGTTGGATTTGTGTAAGCTGTTTAATGCGGCCAAGAGGTATGGGGGGTATGATAAGGTTGTGAAGGAGAAGAAGTGGGGGGAGGTTGTGAGGTTTGTGAGGTCGGCCAGGAAGGTGTCCGAGTGTTCAAAGCATGTTTTGCATCAGTTGTATTTGGAGCATTTGTTTGAGTACGAGGAGTATTATAATAAGCTGAATAAAGAGGGGGCAAGAGGTTGTAAGAGGGGTTTGCAGGAAGAGAAGAATGGTGAATGTTCTAGCTCCAAAAGGAGGCGAACGAGTAATAACGATGGTGAGAGGGCTAAGGTTCGTAAGGTAAAGAAGGAAGAGGAGGAGCATGATCAGATATGTGAGCAATGCAGGAGTGGTCTGCACGGGGAAGTGATGCTTTTGTGCGATAGGTGTGATAAAGGGTGGCACATTTACTGTCTCTCTCCGCCGTTGAAACAGATCCCGTCCGGGAATTGGTACTGCTTAGATTGCTTGAATTCTGATGAAGATTGTTTCGGTTTTGTGCCTGGAAAGCGTTTCTCACTGGAGGCTTTTAGGCGTGTGGCTGATCGGGCCAAGAAGAAATGGTTTGGGTCAGGACCTGCATCTCGGGTGCAAATAGAGAAAAAGTTCTGGGAAATTGTGGAGGGTTCCATCGGTGAAGTTGAAGTTATGTATGGCAGTGACCTGGATACTTCCATTTATGGGAGCGGTTTTCCGCGAGTAAATGACCTGAAACAAGAGTCTGTTGATGCCAAGATATGGGATGAATATTGCGGTAGCCCATGGAATCTCAATAACTTGCCCAAGTTGAAAGGATCAGTTCTTAGAGCTGTTCATAATAACATTACTGGTGTTATGGTGCCTTGGCTCTATATGGGGATGCTGTTTTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTATTCAATGAATTATCATCACTGGGGAGAGCCCAAATGTTGGTACAGTGTCCCTGGTAGTGAAGCCGGTGCTTTTGAGAAGGTGATGCGTAACAGTCTTCCTGATCTTTTTGATGCACAACCTGATTTGCTCTTTCAGCTTGTCACCATGTTAAATCCCTCTGTTTTGCAAGAAAATGGAGTTCCTGTATACAGTGTCCTACAGGAGCCTGGAAATTTTGTCATCACCTTCCCCAGGTCTTACCATGGAGGTTTTAATCTTGGTCTGAATTGTGCAGAGGCTGTCAATTTTGCCCCTGCTGACTGGCTACCTCATGGTGGGTTTGGAGCGGGGCTCTATCAGCTTTATCACAAAACTGCAGTTCTATCTCACGAGGAGCTTGTTTGTGTGCTAGCCAAGAGTGACTGTGACAGCAGAGTATCACCGTATTTGAAGAAAGAATTGATAAGAATATATAACAAGGAGAAAACCTGGAGGGAGCGCCTTTGGAGAAAAGGAATCGTCAAATCATCTCTTATGTCCTCTCGAAAATTCCCTGAATATGTTGGTACTGAGGAGGATCCTACATGCATTATATGCCAGCAATATCTTTACCTCTCTGGTGTGGTTTGTCGTTGTCGGCCATCCACATTTGTATGTCTGGAGCACTCAGAACGTCTTTGTGAATGCAAATCCAGCAGACTTCGTCTTCATTATCGCCATACACTAGCAGAATTGCACGATATGGTGCTTGCAATGGATAAACATGATTGTGAGGAGACAACACAAAGTAGAACAAAGAAAAGACAACTCCAATGCTCTAATGAACCAACTGCGTTAACAAAAAAGGTCAAGGGTGGTCATGCCAGTTTCGCTCAACTTGCGGACCAATGGCTTTTGCGTGCGTGCAAGATTTTTAAGAGTCTGTTTTCTAGAGAAGACTATGTTAATGTACTGAAAGAGGCTGAACAATTTGTCTGGGCCGGTTCAGAGATGAATAATGTTCGGGAAACGGCAAATAATTTGAAAGAAGCTCGGAAATGGGCAGAGGGTGTGAGGAAGTCGGTATCTAAAATTGAAAGTTGGTCATCTAATCACGACAAGGATATAGAGAAGGTTCGTGTGGAATATATCAATGAGTTGCTGAGTTTTGATTCGTTGCCCTGTGACGAGCCTGGGCATCTTATTTTGAAGGGTTACGCTGAAAAGGCAAGGATGTTGATAGAGGAAATCAATACCGCAATGTCTTCATGCTCAAAAGTCCCTGAATTGGAATTGCTGTACAACAGAGTTTGTGAGTTTCCCGTTTATGTAACAGAAAGCGAAGGGTTGCAACAGAAAATTTTGTCTGCAAAGGTTTGGATAGAAGGCATAACAAAATGCATCTCTGAGAAACAGCCTGCTGCAATTGAGCTGGATGTCCTGTACAAGTTGAAATTGGAGATCCCAGAAGTGGAAGTTCAACTCCCACAGATAGAAGTGCTTTCTGATCTAGTAAGAAAAGCTGAATCATGTCAAGCTCAATGCGTCGAAATTTTGAAAGGTCCTATTACCCTCAAGGATGTTGAGGCGCTGCTTCTAGAATGGGATACTTTTTCTGTCAACGTACCGGAGTTGAAGCTTCTGAGGCAGTACCACACTGATGTTGTTTCTTGGAATGCTCGATTGAAAGCTGTTCTTACGAAAATTCATGAAAGAGAGGATCAAGATACTGTAGTTGATGAGTTAGAGCACATTCTAAAGGATGGGGCATCTTTGAAAATCCAAGTTAATCAGATGCCTGCGGTTGAGTTTGAGTTGAAAAAGGCTAGGTGCAGGGAAAGGGCTTTGAGGATGCGAGAAACTATAGTGTCTCTGGACTTCATTCAAGAAGTGATGGTGGATGCACAAGGTTTACATATTGACGGAGAACAAATATTTGTTAATATGTCGAAAGTACTTGATGCTGCCATCCAATGGGAGGAAAGAGCAAAATATATTCTTGCACATGGAGCTCAGATATCTGACTTCGAGGATGTACTTAGGAGTTCGGAAAACATACATGTGACTTTACCTTCTCTCCTTGATGTTAAGGAGGCACTATCAAAGGCTATGGCCTGGTTGAGTAGATCTGAGCCGTTTCTTCTGCATTGTTCTTCATTGGAATCTGCCTCGAGTTCTTTGCTGAAAGTTGACACGTTGAAGGCGTTGATTTCTGAGTCGAAAGATCTAAAAGTATCTATGAAAGAAATCAAGATTCTTGAAACTGTTTTGAGGAACTGTGAGGAATGGAAGCATGATGCTTGCTCTCTACTGCAAGATACAAGGTGCCTATTGGATATGGCGACCAACGGTGAGGGGATAAGCGAGGGGCTTATTTCCAAGATAGAACATGTACTTGCAAGGATAGGATCCATGGAAAATACTGGTTTATCTCTTACGTTTGACTTTGTTGAGCTTGCAAAACTCAAGGATGCTTGTTCTCTGCTGCAATGGTGTAAGAAGGCTATCTCTTTCTGCTTTGCTGTTCCTACTCTCGAGGATATTGAGAGTTTGATAAGTGATGCAGAGACTTCATGTTGTACAGATTCATCTGGTGCACTCTTTGACTCGTTGTTTGAAGGAGTCAAATGGCTTAAGCAAGCAACAAAGATTATTTCTGCACCTAGCAATTCTACAAGTTGCAAGTTGAGTGAAGCTGAAGAAGTTCTTGCTGATTGTCAGAGTATTAATATCTCCTTCCCTCTGACAGTTACTCAAATTGAAGGTGTCATTGATAAACATAAGTCTTGGCTTGAGCAAGTGCATCAATTTTTCAGCCTAAGGGTGGCGGAACGATCTTGGTCTCTTATACTACAGCTAAAGGAGCTTGGGATAGCTGGTGCTTTCAATTGCGCAGAACTAGATTCAATAATATCTGAAGTTGAAAGGGTTCAGAAGTGGAAGAGACAATGCATGGATATTTTCAGAATTGCAGAAGAGAATTCACTGCTCTGTGCTCTAGAAAAGTTACAACAGACTCTGGATAGATCTATGCAAATATATGACAAGGCAAATGGGTTGAGTGAGAAAGGTTCTTATGCATGCTGCTCCGTTGGTTCTCTGGATCAGGAGTTTGTTACCTGTTCTTCGTGCAAGGAATGCTACCATTTGCGGTGCTTAGGATCTTTGACAGTATATGGGAAACACTCGGAGTATGTGTGCTTGTGTTGCCAGTATCTGGTATCTGGAACATTGCAAAATGAAGGAAATCCTCGGGGTTTTGGAGGAGTTCGTCTAGCACTTCAAAAGATTGTTGAACTTTTATCTGAAGAGGATTTCTGTGTTTGTATGGAAGAAAGAGATATATTGAAAGAGGTTTTGAAGAAAGCTCGGGTATGCAAGACACACCTGGAAGCACTAGTGGACTTTGCATTAGCTTATTTGGACAAGGATCTCAGTGTCATATTTGCAAAGCTAGCCACTGCTTTAAAGGCTGTTGAATTAGAAGGCTTGTATGATGATGAAGGTTATTGCAACCTTACATTGGCACTATCAAGATACTCATGGAAGGTCAGAGTTGAAAGATTACTGGAGGGTTCAAAAAAGCCCACAATCTACCAGATTCAACAGCATTTAAAAGAGAGAGTGGCCGTGAACATTCCCCCTGAAGATTACTTTAAGCAAAAACTTACAGAACTGAAGTGCAGTGGCTTGCAGTGGGCAGATAAGGCTAAGAAGGTTGCTGCTGATTCTGGGGCTCTACCCCTGGACAAAGTTTTTGAACTTATTTCTGAGGGTGAAAACTTGCCTGTTCTTGTAGAAAAGGAACTTAAGCTATTGAAAGATCGGAGTATGCTTTATTGCATATGTAGAAAGCCTTATGATCAGAGAGCAATGATTGCATGTGACAAATGTGATGAGTGGTATCACTTTGGTTGTATGAAACTACGTTCCACACCCAAGGTTTACATATGCCCTGCATGTGAGCCTCTAGCAGAAACGTTGCCTACATCATCAGTAGTTCCCTGTACTGATGCCAAATTTGTAGAACCGAAAACGCCCTCCCCCAAGCACACAAAGCCCAGGATGAGCCCAAACAAAGAAGAATTCATTGCAACACAAAAGGTTGCAAGTACAGATGACGCCAACGTGTTTAGGTGTTCCAGTGGGATAGACCGTCTATGGTGGCGAAATCGGAAACCTTTCAGAAGGGTGGCCAAAAAACGAGCTGAGCTTGATTGTCTATCTCTATTTTCTCATGTGCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1839

Amino Acids

209.25

Weight (kDa)

6.28

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 32 - 65 1.8e-16 jmjN domain
ARID PF01388 103 - 188 1.7e-10 ARID/BRIGHT DNA binding domain
PHD PF00628 246 - 290 8.2e-09 PHD-finger
JmjC PF02373 416 - 532 2.2e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 625 - 677 5.4e-14 C5HC2 zinc finger
PLU-1 PF08429 745 - 1031 3e-14 PLU-1-like protein
PLU-1 PF08429 945 - 1192 4.3e-24 PLU-1-like protein
PLU-1 PF08429 1143 - 1411 2.5e-16 PLU-1-like protein
PHD PF00628 1703 - 1743 6.3e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AarI CACCTGC 1 cut(s) 268
AasI GACNNNNNNGTC 1 cut(s) 85
Acc16I TGCGCA 1 cut(s) 4159
Acc36I ACCTGC 2 cut(s) 268, 988
AccB1I GGYRCC 2 cut(s) 1240, 3591
AccB7I CCANNNNNTGG 4 cut(s) 1140, 1344, 3240, 4483
AccBSI CCGCTC 2 cut(s) 1089, 1634
AccI GTMKAC 1 cut(s) 1494
AccII CGCG 1 cut(s) 1099
AclI AACGTT 1 cut(s) 5246
AcoI YGGCCR 5 cut(s) 417, 492, 1925, 4893, 5460
AcsI RAATTY 9 cut(s) 874, 1519, 1835, 2625, 2817, 2970, 4246, 5285, 5351
AcuI CTGAAG 5 cut(s) 3984, 4203, 4593, 4932, 4964
AcyI GRCGYC 1 cut(s) 5388
AflII CTTAAG 2 cut(s) 3899, 5075
AflIII ACRYGT 4 cut(s) 3325, 3459, 3655, 5394
AhlI ACTAGT 1 cut(s) 4663
Alw21I GWGCWC 5 cut(s) 1953, 3018, 3280, 3865, 4264
Alw26I GTCTC 6 cut(s) 821, 2057, 3141, 3827, 4113, 4204
Alw44I GTGCAC 1 cut(s) 3861
AlwNI CAGNNNCTG 4 cut(s) 980, 1586, 4285, 4483
Ama87I CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
ApaI GGGCCC 1 cut(s) 47
ApaLI GTGCAC 1 cut(s) 3861
ApeKI GCWGC 6 cut(s) 2687, 2857, 3230, 3751, 4350, 4988
ApoI RAATTY 9 cut(s) 874, 1519, 1835, 2625, 2817, 2970, 4246, 5285, 5351
ArsI GACNNNNNNTTYG 4 cut(s) 3863, 3895, 5006, 5038
AseI ATTAAT 1 cut(s) 3993
Asp700I GAANNNNTTC 4 cut(s) 1007, 3969, 4194, 5447
AspA2I CCTAGG 1 cut(s) 17
AspLEI GCGC 4 cut(s) 94, 1788, 2857, 4160
AspS9I GGNCC 8 cut(s) 43, 44, 127, 951, 977, 2170, 2265, 2828
AsuC2I CCSGG 1 cut(s) 847
AsuNHI GCTAGC 2 cut(s) 1696, 4720
AvaI CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
AvaII GGWCC 4 cut(s) 127, 977, 2170, 2828
AvrII CCTAGG 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 4085
BaeGI GKGCMC 2 cut(s) 47, 3865
BaeI ACNNNNGTAYC 2 cut(s) 2985, 3018
BalI TGGCCA 1 cut(s) 5462
BamHI GGATCC 2 cut(s) 1864, 3674
BanI GGYRCC 2 cut(s) 1240, 3591
BanII GRGCYC 6 cut(s) 47, 1338, 1641, 3280, 5005, 5339
BauI CACGAG 1 cut(s) 1676
BbsI GAAGAC 6 cut(s) 19, 60, 1394, 1985, 2229, 2523
Bbv12I GWGCWC 5 cut(s) 1953, 3018, 3280, 3865, 4264
BbvCI CCTCAGC 2 cut(s) 159, 359
BbvI GCAGC 6 cut(s) 2674, 2844, 3217, 3738, 4337, 4975
BccI CCATC 6 cut(s) 671, 1040, 1936, 3023, 3154, 3242
BceAI ACGGC 5 cut(s) 154, 811, 2313, 3385, 4880
BcgI CGANNNNNNTGC 2 cut(s) 3427, 3461
BciVI GTATCC 2 cut(s) 1063, 2866
BclI TGATCA 2 cut(s) 727, 5128
BcnI CCSGG 1 cut(s) 847
BcoDI GTCTC 6 cut(s) 821, 2057, 3141, 3827, 4113, 4204
BcuI ACTAGT 1 cut(s) 4663
BfmI CTRYAG 5 cut(s) 1505, 1662, 2997, 3129, 4118
BfoI RGCGCY 2 cut(s) 1789, 2858
BfrI CTTAAG 2 cut(s) 3899, 5075
BfuAI ACCTGC 2 cut(s) 268, 988
BfuI GTATCC 2 cut(s) 1063, 2866
BglII AGATCT 3 cut(s) 3392, 3490, 4292
BisI GCNGC 7 cut(s) 417, 2688, 2858, 3231, 3752, 4351, 4989
BlnI CCTAGG 1 cut(s) 17
BlpI GCTNAGC 2 cut(s) 165, 5475
BlsI GCNGC 7 cut(s) 418, 2689, 2859, 3232, 3753, 4352, 4990
BmcAI AGTACT 1 cut(s) 3222
Bme18I GGWCC 4 cut(s) 127, 977, 2170, 2828
BmeT110I CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
BmgT120I GGNCC 8 cut(s) 43, 44, 127, 951, 977, 2170, 2265, 2828
BmrI ACTGGG 1 cut(s) 1336
BmtI GCTAGC 2 cut(s) 1700, 4724
BmuI ACTGGG 1 cut(s) 1336
BpiI GAAGAC 6 cut(s) 19, 60, 1394, 1985, 2229, 2523
BplI GAGNNNNNCTC 2 cut(s) 1623, 1655
BpmI CTGGAG 5 cut(s) 338, 944, 1798, 1967, 4856
Bpu10I CCTNAGC 4 cut(s) 159, 359, 690, 4423
Bpu1102I GCTNAGC 2 cut(s) 165, 5475
BpuEI CTTGAG 3 cut(s) 2825, 3716, 4079
BpuMI CCSGG 1 cut(s) 847
BsaHI GRCGYC 1 cut(s) 5388
BsaI GGTCTC 1 cut(s) 4113
BsaWI WCCGGW 1 cut(s) 2893
Bse118I RCCGGY 3 cut(s) 94, 1370, 2267
Bse1I ACTGG 9 cut(s) 927, 1234, 1331, 1613, 2151, 3694, 4477, 4839, 5409
Bse21I CCTNAGG 1 cut(s) 4085
Bse3DI GCAATG 8 cut(s) 343, 749, 2046, 2532, 3761, 4496, 5145, 5355
BseMI GCAATG 8 cut(s) 343, 749, 2046, 2532, 3761, 4496, 5145, 5355
BseNI ACTGG 9 cut(s) 927, 1234, 1331, 1613, 2151, 3694, 4477, 4839, 5409
BseRI GAGGAG 9 cut(s) 376, 401, 578, 731, 734, 1694, 1874, 2075, 4545
BseSI GKGCMC 2 cut(s) 47, 3865
BseXI GCAGC 6 cut(s) 2674, 2844, 3217, 3738, 4337, 4975
BsgI GTGCAG 5 cut(s) 743, 1599, 3118, 3909, 4969
Bsh1236I CGCG 1 cut(s) 1099
BshNI GGYRCC 2 cut(s) 1240, 3591
BsiHKAI GWGCWC 5 cut(s) 1953, 3018, 3280, 3865, 4264
BsiHKCI CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
BsiSI CCGG 6 cut(s) 95, 197, 846, 1371, 2268, 2894
BsiWI CGTACG 1 cut(s) 171
BslFI GGGAC 3 cut(s) 140, 1340, 2531
BsmAI GTCTC 6 cut(s) 821, 2057, 3141, 3827, 4113, 4204
BsmFI GGGAC 3 cut(s) 140, 1340, 2531
BsmI GAATGC 3 cut(s) 1976, 2947, 4409
Bso31I GGTCTC 1 cut(s) 4113
BsoBI CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
Bsp120I GGGCCC 1 cut(s) 43
Bsp1407I TGTACA 3 cut(s) 2565, 2709, 3845
Bsp1720I GCTNAGC 2 cut(s) 165, 5475
Bsp19I CCATGG 3 cut(s) 1163, 1549, 3678
BspFNI CGCG 1 cut(s) 1099
BspHI TCATGA 1 cut(s) 2974
BspMAI CTGCAG 1 cut(s) 1666
BspMI ACCTGC 2 cut(s) 268, 988
BspOI GCTAGC 2 cut(s) 1700, 4724
BspT107I GGYRCC 2 cut(s) 1240, 3591
BspTI CTTAAG 2 cut(s) 3899, 5075
BspTNI GGTCTC 1 cut(s) 4113
BsrBI CCGCTC 2 cut(s) 1089, 1634
BsrDI GCAATG 8 cut(s) 343, 749, 2046, 2532, 3761, 4496, 5145, 5355
BsrFI RCCGGY 3 cut(s) 94, 1370, 2267
BsrGI TGTACA 3 cut(s) 2565, 2709, 3845
BsrI ACTGG 9 cut(s) 927, 1234, 1331, 1613, 2151, 3694, 4477, 4839, 5409
BssAI RCCGGY 3 cut(s) 94, 1370, 2267
BssNAI GTATAC 1 cut(s) 1495
BssNI GRCGYC 1 cut(s) 5388
BssSI CACGAG 1 cut(s) 1676
BssT1I CCWWGG 6 cut(s) 17, 1163, 1244, 1549, 3678, 5205
Bst1107I GTATAC 1 cut(s) 1495
Bst2BI CACGAG 1 cut(s) 1676
Bst6I CTCTTC 6 cut(s) 347, 618, 708, 4202, 4236, 4569
BstACI GRCGYC 1 cut(s) 5388
BstAFI CTTAAG 2 cut(s) 3899, 5075
BstAPI GCANNNNNTGC 4 cut(s) 526, 1881, 4404, 5144
BstAUI TGTACA 3 cut(s) 2565, 2709, 3845
BstDSI CCRYGG 3 cut(s) 1163, 1549, 3678
BstENI CCTNNNNNAGG 1 cut(s) 1537
BstFNI CGCG 1 cut(s) 1099
BstH2I RGCGCY 2 cut(s) 1789, 2858
BstHHI GCGC 4 cut(s) 94, 1788, 2857, 4160
BstMAI GTCTC 6 cut(s) 821, 2057, 3141, 3827, 4113, 4204
BstNSI RCATGY 7 cut(s) 524, 1875, 3329, 3659, 4350, 5151, 5229
BstSFI CTRYAG 5 cut(s) 1505, 1662, 2997, 3129, 4118
BstSLI GKGCMC 2 cut(s) 47, 3865
BstUI CGCG 1 cut(s) 1099
BstV1I GCAGC 6 cut(s) 2674, 2844, 3217, 3738, 4337, 4975
BstV2I GAAGAC 6 cut(s) 19, 60, 1394, 1985, 2229, 2523
BstX2I RGATCY 9 cut(s) 837, 1864, 2727, 3392, 3490, 3674, 4292, 4427, 4696
BstYI RGATCY 9 cut(s) 837, 1864, 2727, 3392, 3490, 3674, 4292, 4427, 4696
BstZ17I GTATAC 1 cut(s) 1495
Bsu36I CCTNAGG 1 cut(s) 4085
BsuI GTATCC 2 cut(s) 1063, 2866
BtgI CCRYGG 3 cut(s) 1163, 1549, 3678
BtsI GCAGTG 5 cut(s) 1066, 4250, 4725, 4957, 4967
BveI ACCTGC 2 cut(s) 268, 988
CaiI CAGNNNCTG 4 cut(s) 980, 1586, 4285, 4483
CciI TCATGA 1 cut(s) 2974
CfoI GCGC 4 cut(s) 94, 1788, 2857, 4160
Cfr10I RCCGGY 3 cut(s) 94, 1370, 2267
Cfr13I GGNCC 8 cut(s) 43, 44, 127, 951, 977, 2170, 2265, 2828
CseI GACGC 3 cut(s) 265, 2800, 5396
DraI TTTAAA 2 cut(s) 4734, 4881
DrdI GACNNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 5 cut(s) 417, 492, 1925, 4893, 5460
Eam1104I CTCTTC 6 cut(s) 347, 618, 708, 4202, 4236, 4569
EarI CTCTTC 6 cut(s) 347, 618, 708, 4202, 4236, 4569
EciI GGCGGA 3 cut(s) 183, 813, 4109
Ecl136II GAGCTC 1 cut(s) 3278
Eco130I CCWWGG 6 cut(s) 17, 1163, 1244, 1549, 3678, 5205
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 6 cut(s) 47, 1338, 1641, 3280, 5005, 5339
Eco31I GGTCTC 1 cut(s) 4113
Eco32I GATATC 1 cut(s) 3285
Eco47I GGWCC 4 cut(s) 127, 977, 2170, 2828
Eco53kI GAGCTC 1 cut(s) 3278
Eco57I CTGAAG 5 cut(s) 3984, 4203, 4593, 4932, 4964
Eco81I CCTNAGG 1 cut(s) 4085
Eco88I CYCGRG 5 cut(s) 987, 3436, 3800, 4518, 4635
EcoICRI GAGCTC 1 cut(s) 3278
EcoNI CCTNNNNNAGG 1 cut(s) 1537
EcoO109I RGGNCCY 4 cut(s) 44, 127, 977, 2828
EcoRI GAATTC 3 cut(s) 874, 4246, 5351
EcoRV GATATC 1 cut(s) 3285
EcoT14I CCWWGG 6 cut(s) 17, 1163, 1244, 1549, 3678, 5205
EcoT22I ATGCAT 4 cut(s) 1877, 2669, 4220, 4348
EcoT38I GRGCYC 6 cut(s) 47, 1338, 1641, 3280, 5005, 5339
ErhI CCWWGG 6 cut(s) 17, 1163, 1244, 1549, 3678, 5205
FalI AAGNNNNNCTT 6 cut(s) 759, 791, 2946, 2978, 3957, 3989
FaqI GGGAC 3 cut(s) 140, 1340, 2531
FauI CCCGC 2 cut(s) 341, 1627
FauNDI CATATG 2 cut(s) 5111, 5216
FbaI TGATCA 2 cut(s) 727, 5128
FblI GTMKAC 1 cut(s) 1494
Fnu4HI GCNGC 7 cut(s) 417, 2688, 2858, 3231, 3752, 4351, 4989
FriOI GRGCYC 6 cut(s) 47, 1338, 1641, 3280, 5005, 5339
Fsp4HI GCNGC 7 cut(s) 417, 2688, 2858, 3231, 3752, 4351, 4989
FspI TGCGCA 1 cut(s) 4159
GlaI GCGC 4 cut(s) 93, 1787, 2856, 4159
GluI GCNGC 7 cut(s) 417, 2688, 2858, 3231, 3752, 4351, 4989
GsuI CTGGAG 5 cut(s) 338, 944, 1798, 1967, 4856
HaeII RGCGCY 2 cut(s) 1789, 2858
HapII CCGG 6 cut(s) 95, 197, 846, 1371, 2268, 2894
HgaI GACGC 3 cut(s) 265, 2800, 5396
HhaI GCGC 4 cut(s) 94, 1788, 2857, 4160
Hin1I GRCGYC 1 cut(s) 5388
Hin6I GCGC 4 cut(s) 92, 1786, 2855, 4158
HinP1I GCGC 4 cut(s) 92, 1786, 2855, 4158
HincII GTYRAC 4 cut(s) 78, 2124, 2887, 3457
HindII GTYRAC 4 cut(s) 78, 2124, 2887, 3457
HindIII AAGCTT 1 cut(s) 2903
HpaI GTTAAC 1 cut(s) 2124
HpaII CCGG 6 cut(s) 95, 197, 846, 1371, 2268, 2894
Hpy99I CGWCG 1 cut(s) 2816
HpyCH4IV ACGT 8 cut(s) 242, 1960, 2889, 3461, 3704, 5194, 5246, 5394
HpySE526I ACGT 8 cut(s) 242, 1960, 2889, 3461, 3704, 5194, 5246, 5394
Hsp92I GRCGYC 1 cut(s) 5388
HspAI GCGC 4 cut(s) 92, 1786, 2855, 4158
KflI GGGWCCC 1 cut(s) 127
Ksp22I TGATCA 2 cut(s) 727, 5128
KspAI GTTAAC 1 cut(s) 2124
Lsp1109I GCAGC 6 cut(s) 2674, 2844, 3217, 3738, 4337, 4975
MaeII ACGT 8 cut(s) 242, 1960, 2889, 3461, 3704, 5194, 5246, 5394
MbiI CCGCTC 2 cut(s) 1089, 1634
MfeI CAATTG 2 cut(s) 2691, 4153
MflI RGATCY 9 cut(s) 837, 1864, 2727, 3392, 3490, 3674, 4292, 4427, 4696
MlsI TGGCCA 1 cut(s) 5462
MluNI TGGCCA 1 cut(s) 5462
MlyI GAGTC 6 cut(s) 1130, 2215, 3491, 3866, 3897, 4276
MmeI TCCRAC 2 cut(s) 272, 372
Mox20I TGGCCA 1 cut(s) 5462
Mph1103I ATGCAT 4 cut(s) 1877, 2669, 4220, 4348
MroXI GAANNNNTTC 4 cut(s) 1007, 3969, 4194, 5447
MscI TGGCCA 1 cut(s) 5462
MslI CAYNNNNRTG 4 cut(s) 1939, 2055, 2925, 3326
Msp20I TGGCCA 1 cut(s) 5462
MspCI CTTAAG 2 cut(s) 3899, 5075
MspI CCGG 6 cut(s) 95, 197, 846, 1371, 2268, 2894
MunI CAATTG 2 cut(s) 2691, 4153
Mva1269I GAATGC 3 cut(s) 1976, 2947, 4409
MvnI CGCG 1 cut(s) 1099
NciI CCSGG 1 cut(s) 847
NcoI CCATGG 3 cut(s) 1163, 1549, 3678
NdeI CATATG 2 cut(s) 5111, 5216
NheI GCTAGC 2 cut(s) 1696, 4720
NmuCI GTSAC 7 cut(s) 1061, 1447, 1706, 1712, 2450, 3328, 5150
NsbI TGCGCA 1 cut(s) 4159
NsiI ATGCAT 4 cut(s) 1877, 2669, 4220, 4348
NspI RCATGY 7 cut(s) 524, 1875, 3329, 3659, 4350, 5151, 5229
PaeI GCATGC 1 cut(s) 4350
PaeR7I CTCGAG 2 cut(s) 3436, 3800
PagI TCATGA 1 cut(s) 2974
PaqCI CACCTGC 1 cut(s) 268
PceI AGGCCT 1 cut(s) 286
PciI ACATGT 2 cut(s) 3325, 3655
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 3467
PctI GAATGC 3 cut(s) 1976, 2947, 4409
PdmI GAANNNNTTC 4 cut(s) 1007, 3969, 4194, 5447
Pfl23II CGTACG 1 cut(s) 171
PflFI GACNNNGTC 1 cut(s) 1989
PflMI CCANNNNNTGG 4 cut(s) 1140, 1344, 3240, 4483
PfoI TCCNGGA 1 cut(s) 845
PkrI GCNGC 7 cut(s) 418, 2689, 2859, 3232, 3753, 4352, 4990
PleI GAGTC 6 cut(s) 1129, 2214, 3490, 3866, 3896, 4276
PpsI GAGTC 6 cut(s) 1129, 2214, 3490, 3866, 3896, 4276
PpuMI RGGWCCY 3 cut(s) 127, 977, 2828
PscI ACATGT 2 cut(s) 3325, 3655
PshBI ATTAAT 1 cut(s) 3993
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 3280
Psp1406I AACGTT 1 cut(s) 5246
Psp5II RGGWCCY 3 cut(s) 127, 977, 2828
PspLI CGTACG 1 cut(s) 171
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 8 cut(s) 43, 44, 127, 951, 977, 2170, 2265, 2828
PspPPI RGGWCCY 3 cut(s) 127, 977, 2828
PspXI VCTCGAGB 1 cut(s) 3436
PstI CTGCAG 1 cut(s) 1666
PstNI CAGNNNCTG 4 cut(s) 980, 1586, 4285, 4483
PsuI RGATCY 9 cut(s) 837, 1864, 2727, 3392, 3490, 3674, 4292, 4427, 4696
PsyI GACNNNGTC 1 cut(s) 1989
RseI CAYNNNNRTG 4 cut(s) 1939, 2055, 2925, 3326
SacI GAGCTC 1 cut(s) 3280
SatI GCNGC 7 cut(s) 417, 2688, 2858, 3231, 3752, 4351, 4989
Sau96I GGNCC 8 cut(s) 43, 44, 127, 951, 977, 2170, 2265, 2828
ScaI AGTACT 1 cut(s) 3222
SchI GAGTC 6 cut(s) 1130, 2215, 3491, 3866, 3897, 4276
SfcI CTRYAG 5 cut(s) 1505, 1662, 2997, 3129, 4118
Sfr274I CTCGAG 2 cut(s) 3436, 3800
SgrAI CRCCGGYG 1 cut(s) 94
SinI GGWCC 4 cut(s) 127, 977, 2170, 2828
SlaI CTCGAG 2 cut(s) 3436, 3800
SmiMI CAYNNNNRTG 4 cut(s) 1939, 2055, 2925, 3326
SmlI CTYRAG 7 cut(s) 2840, 3436, 3731, 3800, 3899, 4058, 5075
SmoI CTYRAG 7 cut(s) 2840, 3436, 3731, 3800, 3899, 4058, 5075
SpeI ACTAGT 1 cut(s) 4663
SphI GCATGC 1 cut(s) 4350
SseBI AGGCCT 1 cut(s) 286
SspI AATATT 2 cut(s) 1151, 3201
SstI GAGCTC 1 cut(s) 3280
StuI AGGCCT 1 cut(s) 286
StyI CCWWGG 6 cut(s) 17, 1163, 1244, 1549, 3678, 5205
TaiI ACGT 8 cut(s) 245, 1963, 2892, 3464, 3707, 5197, 5249, 5397
TaqI TCGA 8 cut(s) 1831, 2814, 2947, 3215, 3294, 3437, 3485, 3801
TatI WGTACW 9 cut(s) 2236, 2565, 2709, 3220, 3301, 3658, 3845, 5273, 5378
TauI GCSGC 1 cut(s) 419
TseFI GTSAC 7 cut(s) 1061, 1447, 1706, 1712, 2450, 3328, 5150
TseI GCWGC 6 cut(s) 2687, 2857, 3230, 3751, 4350, 4988
Tsp45I GTSAC 7 cut(s) 1061, 1447, 1706, 1712, 2450, 3328, 5150
TspGWI ACGGA 2 cut(s) 3204, 4345
Tth111I GACNNNGTC 1 cut(s) 1989
Van91I CCANNNNNTGG 4 cut(s) 1140, 1344, 3240, 4483
Vha464I CTTAAG 2 cut(s) 3899, 5075
VneI GTGCAC 1 cut(s) 3861
VpaK11BI GGWCC 4 cut(s) 127, 977, 2170, 2828
VspI ATTAAT 1 cut(s) 3993
XagI CCTNNNNNAGG 1 cut(s) 1537
XapI RAATTY 9 cut(s) 874, 1519, 1835, 2625, 2817, 2970, 4246, 5285, 5351
XbaI TCTAGA 3 cut(s) 2216, 2863, 4264
XceI RCATGY 7 cut(s) 524, 1875, 3329, 3659, 4350, 5151, 5229
XhoI CTCGAG 2 cut(s) 3436, 3800
XmaJI CCTAGG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 1494
XmnI GAANNNNTTC 4 cut(s) 1007, 3969, 4194, 5447
ZrmI AGTACT 1 cut(s) 3222
Zsp2I ATGCAT 4 cut(s) 1877, 2669, 4220, 4348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.