MD14G1103700.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004720
Physical Location & Seq
Reverse (-)
15999174 .. 16014561
15388 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1103700.v1.1.491

Sequence Viewer

Length: 5529 bp
ATGGGAAAGGGAAAACCCAGGGCTGTGGAGAAGGGAGTGGTAGGTCAAAATTTGAGTGTTACAGCATCTGGATCATTGAATATTCCATCTGCACCTGTGTATTACCCTAGTGAGGATGAATTTAGAGACCCTTTGGAGTATATTTATAAGATCAGGCCAGAGGCCGAGGCGTATGGGATTTGTAGGATAGTTCCGCCGAAGTGCTGGAAACCCCCATTTGCGTTGGATTTGGATTTGTTTACGTTTCCTACAAAGACGCAGGCCATTCACCAGCTTCAGGTGCGGCCTGCGTCTTGCGATTCCAAGACTTTTGAGTTGGAATACAGTAGGTTTTGGGAGGACCACTGTGGGAAGAAGTTGCGGAAGAGGGTGGTTTTTGAAGGGGAGGAGTTGGATTTGTGTAAATTATTTAATGCTGTGAAGCGGTATGGAGGATATGATAAGGTTGTCAAGGGGAAGAAGTGGGCGGAGGTTGCTCGTTTTGTGACGCCGCTGATAAAGATTTCGGAGTGCTCGAAGCACGTATTGTGTCAACTGTATCGAGACCATTTGTTTGATTATGAAAAGTATTATAACAAGCTGAACAAAGAGGGGGCTAGGAGATGTAAGAGGGTTATGCCTGTGGAAAAGAGTGGACAGAGTTTTGAATGTTCCAGCTCCAAAAGGAGGCGAGTCAATAATGGTGGTGAGAAAGTTAAGGTCGGTAAGGTAGAGAAGGAGGATGAGGAGCATGATCAGATATGTGAACAATGCAGAAGTGGTCTGCACGGGGAAGTGATGCTTTTGTGTGATAGGTGTAATAAGGGGTGGCACATTTATTGCCTGTCTCCTCCATTGAAACAGGTTCCGCCAGGGAATTGGTATTGTTTGGATTGCTTGAATTCTGACAAGGATAGTTTTGGCTTTGTTCCTGGTAAGCAGTTCTCGCTGGAAGTTTTTAGGCGTATGGCTAATCGCTCCAAGAGGAAATGGTTTGGGGCAGGATCTGCATCAAGGGTGCAAATAGAGAAAAAGTTTTGGGAGATTGTGGAGGGTTCAGTCGGTGAGGTGGAAGTTAAGTATGGCAGTGACTTGGATACTTCCATATATGGGAGTGGGTTTCCACGTGTAAATGACCAGAAACCAGAATCAGCTGAGGCCAAGATATGGGATGAGTACTGCAGTAGCCCATGGAATCTCAATAACTTGCCCAAATTGAAAGGATCAGTACTTCAAGCTGTTCATCATAACATTGCTGGTGTTATGGTTCCTTGGCTGTATGTTGGGATGTTGTTCTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTACTCAATGAATTATCACCACTGGGGAGAGCCAAAATGTTGGTACAGCGTCCCTGGTAGTGAAGCCAGTGCATTTGAGAAGGTGATGCGCAACAGTCTTCCTGATCTTTTTGATGCACAACCCGATTTACTCTTTCAGCTTGTCACCATGTTAAATCCATCTGTCTTGCAAGAGAATGGAGTTCCTGTATATAGTGTGCTACAGGAGCCTGGGAATTTTGTCATCACCTTCCCCAGATCTTACCATGGAGGCTTTAATCTTGGTTTGAACTGTGCAGAAGCTGTCAATTTTGCTCCTGCGGACTGGCTACCTCATGGTGGGTTTGGAGCAGGGCTGTATCAGCTTTATCACAAAACTGCCGTTCTGTCTCACGAGGAGCTTGTTTGTGTGCTAGCCAAGAGTGACTGTGACAGCAGAGTATCAACTTATTTGAAGAAAGAATTGACTAGAATCTACAATAAGGAAAAAACCTGGAGGGAGCGCCTTTGGAGGAAAGGGATCATCAGATCGTCCCTTATGTCCTCTCGGACATGCCCTGAGTATGTTGGTACCGAAGAGGATCCTACATGCATTATATGCCAACAGTATCTTTTTCTTTCTGCTGTTGTTTGTCGTTGTAGGCCATCTGCATTTGTATGTCTGGAGCATTGGGAACACCTTTGCGAATGCAAATCCAGAAGACTCCGTCTTTTATATCGTCATTCTCTGGCAGAGTTGCATGAATTGGTCCTCGAAATGGATAAACATGGTTTTGAGGAGACAACACAAAGTAGGACTATAAGAAGGCATATCTCATGCACTGATGAACCAACGGCTTTGACAAAAAAGGTCAAAGGGGGCCATGCCACTTTTGCTCAACTTGCAGAACAATGGCTTTTGCGCTCATGCAAGATTTCTCAGAATCCATTTTCAAGAGATGAGTATGTGTCTTTATTAAAAGAAGCTGAACAGTTTCTTTGGGCTGGCTCAGAGATGAATCCTGTGCGGGAAACAACCAAGAATTTGATCAGATTTCAGAAATGGGCTGAAGGTGTAAGAGATTGTCTATCTAAACTTGAAACTTGGTCATCTCATCATGGAAACAATATCGAGAAAGTTCACTTGGATTATATCAATGAGTTGCTGAGTTTTGATGCGGTGCCCTGTTACGAGACTGGGCATCATAATTTGAAGGGCTATGCAGAAAAGGCAAAAATGTTGATACAGGAAATTGAATCTGCTATGTCATCATGCCTAAAGATATCGGAGTTGAAATTGTTGTATTCTAGAGCCTGTGAGCTTCCAGTCTATGTGAAAGAAAGTGAAAATTTACTACAAAAAATTTCATCTGCAGAGGTCTTGATGGAAGGCATAAGAAAATGTATCTCAGAGACACGTACTGCAGCAATTGGGATTGATGTTGTTTACAATTTGAAATCAGAGATTTCAGAGCTCCAAGTTGAACTCCCAGACATGGATAGGCTTTCAGATCTATTAAGGACGGCTGAATCATGGCGTGCTCGATGTGGTGAAATTTTGAAAGGTCCTATTAGCCTTGAGGATGTTGAGGCGCTTCTGAAACAATTGGATGGTTTTACTGTAAACACACCAGAGCTGAAGCTTCTGAAGCAGTACCACATTGATACAGTTTCTTGGATTTCTCGTCTTAATGCTGTTCTTGTGAACATTCACAAAAGGGAGGATCAAACTAATGTAGTTAATGAGTTAATGCTCATATTAACAGATGGAGCATCTTTGAAAATTAAAGTTGATCAGTTGTCTATCGTAGAGTTTGAGTTGAAGAAGGCTCAGTGCAGGGAAAAGGCGCTGAGGATGCGAGATACTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGATGGAGGCAAGAATGTTACATGTTGAGGGGGAGAAGTTATTTGTTGATATGTCTGAAGTGTTAGCTGTTGCCATGCTGTGGGAGGAAAGAGCGAAATATATCCTTGCACACAAGGCTCAGATATCTGACTTTGAGGATGTTATCAGGAGTTCGGAAGACATACATGTGCATTTATCTTCTCTCCATGATGTCAAGGATGCACTATCAAAGGCTAAGATCTGGTTGAGGAAGTCAAAGCCATTTTTAATGACTTCTCCTGTGGTATCTGTTTCAAGTTCTTTGCTGAATGTCGACACCTTGAAGGAATTAGTTTCTGAGTCAAAGCCTCTGAAGGTTTCTTTGAAAGAAATAAGGATGCTTGAGACTGTTTTGATGAACTGTAAGGAATGGGAGAATGGCGCTTGCTGTCTACTGCAAGATACAAGGTGCCTATTTGATATGAGGATTGTTGATGATGGAACAAGAGATGGTCTTCTTTCTAAGATTGAACATCTAATTGCAAGAATCAAATCCATGGGAAGCACTGGTTTATCTCTTTCCTTTGACTTTGGCGAGCTTGTGAAACTTAAGGAGGCTTGTTCTGTGCTGCAATGGTGTAAGAAGGCTCTTTCTTTCAGCATTGGAGTTCCTCCCCTTGAGGATGTTGAAAATTTGGCGAGTGCTGCAGAAAATTTATGTGGTACATTTGCCCCTAGTGCATTGTGGGGCTCGTTGGTTGAAGGGCTGAAATGGCTTGAGCATGCTACAAAGGTTATTTCTGCATCATGCAATTCTAAAAGATGCACTTTAAGTGAAGCTGAAGAAGTTCTTTCTAAATCTCAGAGTATCAGTGTCTCCTTCCCATTGATGGTTGGTCAACTTGAATGTGCTATTCAGAAACACATGTCCTGGCTTGAGCAAGTGCATCAACTTTTCAGCCTAAGGCCAGGAGAACGATCTTGGTCTCTAACATTACAGCTAAAGGAACTTGGAGTTTCTGCTGCTTTCAGTTGCACAGAACTAGATTTGATTGTATCTGAAGTTGAAAGGGTTGAGAGTTGGAAACAACAGTGCATGGATATTTTCAGGATAGTAGTTGGAGATGATAATTCATTGCTTGGCGGTTTAGAAAAGATAGGACAGACTCTAAAGAGATGTATACACATATATGAGAAACCACATGGCTTGAAAGAGAGTAGTTCTTTTGCTTGCTGCTCCAGTGGTTCTCTGGATCAGGAATTTCTCACTTGTTCTTCATGCAAGGACTGCTACCATTTGCGGTGTCTAGGGTCTTCCATGGTAGGTGGAAAACATGCAGAATATGAATGCCCATGTTGCCGATATCTGGTATCTGGAACTTCTTCTCGAAGTGGGCTCAGTACTCTGAAACTTGGTGGGATGCGTCCAGAACTTCAAAAAATTATTGAACATTTATCTGGTGAAGAAGACTTCTGTGTTTGTATTAAAGAAAGAGATGTACTGAAAGAAGTTTTGAAGCAAGCTCTAGCATGCAAATCTCGCTTGACAGAATTAGTGGATTTTGCATTAGCTTATTCTGATAAGGATCTCAGTGTGCTCGTTGAAAAACTAACAACAGCTTTAAAGGCCAGAGAAATGCAGGGTGTGCATGATCATGAAGGTGATATCAACCTTATGTTGGCTTTATCAAGATACTCATGGAAAGTCAGAGTCAATAGGTTATTGGAGGGTTCACAAAAGCCCACAATCCAACAGGTTCAGCAGCAGTTAAAAGAGGGAGCTGCCTTGAACATTCCGCCTTCGGATTATTATTGGCAGAAACTTACAGAAGTGAGGATTATTGGTTTGCAGTGGGCAGATACTGCTAAGAAGGTTGCTGCCGATTCTGGGGCTCTCCCCTTGGATAAAGTTTTTGAACTTGTTTCGGAGGGTGAAAATTTGCCTGTTCGTCTAGAGAAGGAACTTAAGTTGTTGAAAGCTCGGAGTATGCTTTATTGCATTTGCCGAAAGCCGTATGATCAACGAGCAATGATTGCGTGTGACCAATGTAATGAGTGGTATCACTTTGATTGCATTAAATTACGTTCCACACCAAAGGTTTACATATGCCCTGCATGTCAGCCTCTAGGAGAAGAGTTGTCTTGTGAACCCGCGGTGGATCAAGAGAGATGTACTGATGCGAAATCTGTAGAGCCCAAAACGCCCTCTCCTACGCATGCAAAGTGCAGGACGAACCCAAAGAAGGCGGAGTCCGGTCTAGCACAAAAGAACCCCAACAATGTTTATAGGTGTTCTAGCGGGATAGAACGTTTATGGTGGCGAAATCGGAAACCTTTCAGAAGGGCGGCTAAACGACGTGCCAAGCTTGATAGTCTCTCTCTGTTTTCTCATTTGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1843

Amino Acids

209.35

Weight (kDa)

7.67

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 32 - 65 1.1e-15 jmjN domain
ARID PF01388 102 - 188 3.1e-12 ARID/BRIGHT DNA binding domain
PHD PF00628 248 - 292 1.9e-08 PHD-finger
JmjC PF02373 418 - 534 3.9e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 627 - 679 3.1e-14 C5HC2 zinc finger
PLU-1 PF08429 897 - 989 1.3e-07 PLU-1-like protein
PLU-1 PF08429 997 - 1189 1.3e-15 PLU-1-like protein
PLU-1 PF08429 1149 - 1411 8.5e-13 PLU-1-like protein
PLU-1 PF08429 1600 - 1702 2.9e-06 PLU-1-like protein
PHD PF00628 1708 - 1748 3.5e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 573
Acc16I TGCGCA 1 cut(s) 1400
Acc65I GGTACC 1 cut(s) 1859
AccB1I GGYRCC 3 cut(s) 1859, 2449, 3594
AccB7I CCANNNNNTGG 2 cut(s) 1146, 3246
AccI GTMKAC 3 cut(s) 3459, 3577, 4306
AccII CGCG 1 cut(s) 5279
AclI AACGTT 1 cut(s) 5434
AcuI CTGAAG 8 cut(s) 260, 2358, 2927, 2936, 3243, 3518, 3987, 4206
AcvI CACGTG 1 cut(s) 1106
AcyI GRCGYC 1 cut(s) 488
AflII CTTAAG 2 cut(s) 3734, 5090
AflIII ACRYGT 5 cut(s) 1105, 2684, 3187, 3331, 4050
AjiI CACGTC 1 cut(s) 5483
AjnI CCWGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
Alw21I GWGCWC 4 cut(s) 515, 2745, 2812, 4725
AlwNI CAGNNNCTG 4 cut(s) 68, 986, 1592, 4988
AoxI GGCC 9 cut(s) 155, 162, 261, 284, 1137, 1931, 2149, 4091, 4752
ApeKI GCWGC 8 cut(s) 2693, 3754, 3830, 4148, 4359, 4888, 4907, 5003
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
Asp700I GAANNNNTTC 4 cut(s) 2262, 3972, 4507, 5459
Asp718I GGTACC 1 cut(s) 1859
AspLEI GCGC 6 cut(s) 1401, 1794, 2193, 2863, 3118, 3569
AspS9I GGNCC 4 cut(s) 340, 2038, 2149, 2834
AsuNHI GCTAGC 1 cut(s) 1702
AvaII GGWCC 3 cut(s) 340, 2038, 2834
AxyI CCTNAGG 1 cut(s) 4088
BaeGI GKGCMC 1 cut(s) 2454
BamHI GGATCC 1 cut(s) 1870
BanI GGYRCC 3 cut(s) 1859, 2449, 3594
BanII GRGCYC 5 cut(s) 2745, 3878, 4524, 5020, 5322
BauI CACGAG 1 cut(s) 1682
BbrPI CACGTG 1 cut(s) 1106
BbsI GAAGAC 6 cut(s) 1400, 1996, 3330, 3632, 4431, 4599
Bbv12I GWGCWC 4 cut(s) 515, 2745, 2812, 4725
BbvCI CCTCAGC 2 cut(s) 1134, 3119
BbvI GCAGC 8 cut(s) 2705, 3741, 3817, 4135, 4346, 4894, 4900, 4990
BceAI ACGGC 4 cut(s) 1655, 2139, 2808, 5122
BciT130I CCWGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
BciVI GTATCC 1 cut(s) 1069
BclI TGATCA 5 cut(s) 733, 2316, 3061, 4777, 5143
BfmI CTRYAG 6 cut(s) 1159, 1511, 2640, 2691, 3831, 5313
BfoI RGCGCY 4 cut(s) 1795, 2864, 3119, 3570
BfrI CTTAAG 2 cut(s) 3734, 5090
BfuI GTATCC 1 cut(s) 1069
BglII AGATCT 3 cut(s) 1547, 2779, 3384
BmcAI AGTACT 3 cut(s) 1157, 1209, 4528
Bme1390I CCNGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
Bme18I GGWCC 3 cut(s) 340, 2038, 2834
BmgBI CACGTC 1 cut(s) 5483
BmgT120I GGNCC 4 cut(s) 340, 2038, 2149, 2834
BmiI GGNNCC 8 cut(s) 846, 1248, 1518, 1861, 1872, 2150, 2451, 3596
BmrFI CCNGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
BmrI ACTGGG 2 cut(s) 1342, 2475
BmtI GCTAGC 1 cut(s) 1706
BmuI ACTGGG 2 cut(s) 1342, 2475
BpiI GAAGAC 6 cut(s) 1400, 1996, 3330, 3632, 4431, 4599
BpmI CTGGAG 3 cut(s) 1804, 1973, 4348
Bpu10I CCTNAGC 2 cut(s) 1134, 3119
BpuEI CTTGAG 5 cut(s) 2867, 3548, 3824, 3923, 4082
BsaAI YACGTR 3 cut(s) 523, 1106, 2687
BsaBI GATNNNNATC 2 cut(s) 988, 4710
BsaHI GRCGYC 1 cut(s) 488
BsaI GGTCTC 3 cut(s) 120, 537, 4116
BsaWI WCCGGW 1 cut(s) 5378
BsaXI ACNNNNNCTCC 4 cut(s) 1482, 1512, 5317, 5347
Bse1I ACTGG 7 cut(s) 1337, 1377, 1619, 2470, 2594, 3697, 4365
Bse21I CCTNAGG 1 cut(s) 4088
Bse3DI GCAATG 4 cut(s) 1230, 3764, 4259, 5160
Bse8I GATNNNNATC 2 cut(s) 988, 4710
BseBI CCWGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
BseJI GATNNNNATC 2 cut(s) 988, 4710
BseMI GCAATG 4 cut(s) 1230, 3764, 4259, 5160
BseNI ACTGG 7 cut(s) 1337, 1377, 1619, 2470, 2594, 3697, 4365
BseRI GAGGAG 5 cut(s) 401, 740, 819, 1700, 2081
BseSI GKGCMC 1 cut(s) 2454
BseXI GCAGC 8 cut(s) 2705, 3741, 3817, 4135, 4346, 4894, 4900, 4990
BsgI GTGCAG 5 cut(s) 75, 749, 1605, 3124, 5371
Bsh1236I CGCG 1 cut(s) 5279
BshFI GGCC 9 cut(s) 157, 164, 263, 286, 1139, 1933, 2151, 4093, 4754
BshNI GGYRCC 3 cut(s) 1859, 2449, 3594
BsiHKAI GWGCWC 4 cut(s) 515, 2745, 2812, 4725
BsiSI CCGG 1 cut(s) 5379
BslFI GGGAC 2 cut(s) 1346, 1807
BsmFI GGGAC 2 cut(s) 1346, 1807
BsmI GAATGC 2 cut(s) 1982, 4478
BsnI GGCC 9 cut(s) 157, 164, 263, 286, 1139, 1933, 2151, 4093, 4754
Bso31I GGTCTC 3 cut(s) 120, 537, 4116
Bsp1286I GDGCHC 9 cut(s) 515, 2454, 2745, 2812, 3878, 4524, 4725, 5020, 5322
Bsp19I CCATGG 4 cut(s) 1169, 1555, 3681, 4443
BspANI GGCC 9 cut(s) 157, 164, 263, 286, 1139, 1933, 2151, 4093, 4754
BspFNI CGCG 1 cut(s) 5279
BspHI TCATGA 1 cut(s) 4780
BspLI GGNNCC 8 cut(s) 846, 1248, 1518, 1861, 1872, 2150, 2451, 3596
BspMAI CTGCAG 4 cut(s) 1163, 2644, 2695, 3835
BspOI GCTAGC 1 cut(s) 1706
BspT107I GGYRCC 3 cut(s) 1859, 2449, 3594
BspTI CTTAAG 2 cut(s) 3734, 5090
BspTNI GGTCTC 3 cut(s) 120, 537, 4116
BsrDI GCAATG 4 cut(s) 1230, 3764, 4259, 5160
BsrI ACTGG 7 cut(s) 1337, 1377, 1619, 2470, 2594, 3697, 4365
BssNAI GTATAC 1 cut(s) 4307
BssNI GRCGYC 1 cut(s) 488
BssSI CACGAG 1 cut(s) 1682
BssT1I CCWWGG 6 cut(s) 1169, 1250, 1555, 3681, 4443, 5025
Bst1107I GTATAC 1 cut(s) 4307
Bst2BI CACGAG 1 cut(s) 1682
Bst2UI CCWGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
Bst6I CTCTTC 3 cut(s) 359, 1860, 5253
BstACI GRCGYC 1 cut(s) 488
BstAFI CTTAAG 2 cut(s) 3734, 5090
BstAPI GCANNNNNTGC 6 cut(s) 986, 1887, 4413, 4771, 4988, 5159
BstBAI YACGTR 3 cut(s) 523, 1106, 2687
BstDSI CCRYGG 5 cut(s) 1169, 1555, 3681, 4443, 5277
BstFNI CGCG 1 cut(s) 5279
BstH2I RGCGCY 4 cut(s) 1795, 2864, 3119, 3570
BstHHI GCGC 6 cut(s) 1401, 1794, 2193, 2863, 3118, 3569
BstNI CCWGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
BstSCI CCNGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
BstSFI CTRYAG 6 cut(s) 1159, 1511, 2640, 2691, 3831, 5313
BstSLI GKGCMC 1 cut(s) 2454
BstUI CGCG 1 cut(s) 5279
BstV1I GCAGC 8 cut(s) 2705, 3741, 3817, 4135, 4346, 4894, 4900, 4990
BstV2I GAAGAC 6 cut(s) 1400, 1996, 3330, 3632, 4431, 4599
BstX2I RGATCY 6 cut(s) 983, 1547, 1870, 2779, 3384, 4711
BstXI CCANNNNNNTGG 3 cut(s) 25, 858, 1350
BstYI RGATCY 6 cut(s) 983, 1547, 1870, 2779, 3384, 4711
BstZ17I GTATAC 1 cut(s) 4307
Bsu36I CCTNAGG 1 cut(s) 4088
BsuI GTATCC 1 cut(s) 1069
BsuRI GGCC 9 cut(s) 157, 164, 263, 286, 1139, 1933, 2151, 4093, 4754
BtgI CCRYGG 5 cut(s) 1169, 1555, 3681, 4443, 5277
BtrI CACGTC 1 cut(s) 5483
BtsI GCAGTG 2 cut(s) 1072, 4982
CaiI CAGNNNCTG 4 cut(s) 68, 986, 1592, 4988
CciI TCATGA 1 cut(s) 4780
CfoI GCGC 6 cut(s) 1401, 1794, 2193, 2863, 3118, 3569
Cfr13I GGNCC 4 cut(s) 340, 2038, 2149, 2834
Cfr42I CCGCGG 1 cut(s) 5280
CseI GACGC 5 cut(s) 265, 279, 496, 1348, 4538
CspCI CAANNNNNGTGG 4 cut(s) 664, 699, 4314, 4349
DraI TTTAAA 1 cut(s) 4749
Eam1104I CTCTTC 3 cut(s) 359, 1860, 5253
EarI CTCTTC 3 cut(s) 359, 1860, 5253
EciI GGCGGA 5 cut(s) 183, 482, 837, 4911, 5387
Ecl136II GAGCTC 1 cut(s) 2743
Eco130I CCWWGG 6 cut(s) 1169, 1250, 1555, 3681, 4443, 5025
Eco24I GRGCYC 5 cut(s) 2745, 3878, 4524, 5020, 5322
Eco31I GGTCTC 3 cut(s) 120, 537, 4116
Eco32I GATATC 4 cut(s) 2553, 3291, 4490, 4792
Eco47I GGWCC 3 cut(s) 340, 2038, 2834
Eco53kI GAGCTC 1 cut(s) 2743
Eco57I CTGAAG 8 cut(s) 260, 2358, 2927, 2936, 3243, 3518, 3987, 4206
Eco72I CACGTG 1 cut(s) 1106
Eco81I CCTNAGG 1 cut(s) 4088
EcoICRI GAGCTC 1 cut(s) 2743
EcoO109I RGGNCCY 1 cut(s) 2834
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
EcoRV GATATC 4 cut(s) 2553, 3291, 4490, 4792
EcoT14I CCWWGG 6 cut(s) 1169, 1250, 1555, 3681, 4443, 5025
EcoT22I ATGCAT 1 cut(s) 1883
EcoT38I GRGCYC 5 cut(s) 2745, 3878, 4524, 5020, 5322
ErhI CCWWGG 6 cut(s) 1169, 1250, 1555, 3681, 4443, 5025
FalI AAGNNNNNCTT 6 cut(s) 765, 797, 3937, 3969, 3960, 3992
FaqI GGGAC 2 cut(s) 1346, 1807
FauI CCCGC 3 cut(s) 2289, 5284, 5417
FauNDI CATATG 1 cut(s) 5231
FbaI TGATCA 5 cut(s) 733, 2316, 3061, 4777, 5143
FblI GTMKAC 3 cut(s) 3459, 3577, 4306
FriOI GRGCYC 5 cut(s) 2745, 3878, 4524, 5020, 5322
FspI TGCGCA 1 cut(s) 1400
GlaI GCGC 6 cut(s) 1400, 1793, 2192, 2862, 3117, 3568
GsuI CTGGAG 3 cut(s) 1804, 1973, 4348
HaeII RGCGCY 4 cut(s) 1795, 2864, 3119, 3570
HaeIII GGCC 9 cut(s) 157, 164, 263, 286, 1139, 1933, 2151, 4093, 4754
HapII CCGG 1 cut(s) 5379
HgaI GACGC 5 cut(s) 265, 279, 496, 1348, 4538
HhaI GCGC 6 cut(s) 1401, 1794, 2193, 2863, 3118, 3569
Hin1I GRCGYC 1 cut(s) 488
Hin6I GCGC 6 cut(s) 1399, 1792, 2191, 2861, 3116, 3567
HinP1I GCGC 6 cut(s) 1399, 1792, 2191, 2861, 3116, 3567
HincII GTYRAC 3 cut(s) 533, 3460, 4025
HindII GTYRAC 3 cut(s) 533, 3460, 4025
HindIII AAGCTT 2 cut(s) 2909, 5489
HpaII CCGG 1 cut(s) 5379
Hpy99I CGWCG 1 cut(s) 5484
HpyCH4IV ACGT 7 cut(s) 242, 522, 1105, 2686, 5209, 5434, 5482
HpySE526I ACGT 7 cut(s) 242, 522, 1105, 2686, 5209, 5434, 5482
Hsp92I GRCGYC 1 cut(s) 488
HspAI GCGC 6 cut(s) 1399, 1792, 2191, 2861, 3116, 3567
KpnI GGTACC 1 cut(s) 1863
Ksp22I TGATCA 5 cut(s) 733, 2316, 3061, 4777, 5143
KspI CCGCGG 1 cut(s) 5280
Lsp1109I GCAGC 8 cut(s) 2705, 3741, 3817, 4135, 4346, 4894, 4900, 4990
MaeII ACGT 7 cut(s) 242, 522, 1105, 2686, 5209, 5434, 5482
MaeIII GTNAC 9 cut(s) 58, 484, 1067, 1453, 1712, 1718, 2456, 3183, 5165
MfeI CAATTG 2 cut(s) 2697, 2873
MflI RGATCY 6 cut(s) 983, 1547, 1870, 2779, 3384, 4711
MhlI GDGCHC 9 cut(s) 515, 2454, 2745, 2812, 3878, 4524, 4725, 5020, 5322
MlyI GAGTC 6 cut(s) 681, 1986, 3494, 4285, 4845, 5384
MmeI TCCRAC 6 cut(s) 204, 297, 372, 4187, 4225, 4900
Mph1103I ATGCAT 1 cut(s) 1883
MroXI GAANNNNTTC 4 cut(s) 2262, 3972, 4507, 5459
MslI CAYNNNNRTG 5 cut(s) 1945, 3332, 4314, 4779, 4785
MspA1I CMGCKG 3 cut(s) 493, 1133, 5279
MspCI CTTAAG 2 cut(s) 3734, 5090
MspI CCGG 1 cut(s) 5379
MspR9I CCNGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
MunI CAATTG 2 cut(s) 2697, 2873
Mva1269I GAATGC 2 cut(s) 1982, 4478
MvaI CCWGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
MvnI CGCG 1 cut(s) 5279
NcoI CCATGG 4 cut(s) 1169, 1555, 3681, 4443
NdeI CATATG 1 cut(s) 5231
NheI GCTAGC 1 cut(s) 1702
NlaIV GGNNCC 8 cut(s) 846, 1248, 1518, 1861, 1872, 2150, 2451, 3596
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 6 cut(s) 484, 1067, 1453, 1712, 1718, 5165
NsbI TGCGCA 1 cut(s) 1400
NsiI ATGCAT 1 cut(s) 1883
PaeI GCATGC 3 cut(s) 3911, 4659, 5345
PagI TCATGA 1 cut(s) 4780
PasI CCCWGGG 1 cut(s) 18
PciI ACATGT 3 cut(s) 3187, 3331, 4050
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PctI GAATGC 2 cut(s) 1982, 4478
PdmI GAANNNNTTC 4 cut(s) 2262, 3972, 4507, 5459
PflFI GACNNNGTC 1 cut(s) 1995
PflMI CCANNNNNTGG 2 cut(s) 1146, 3246
PleI GAGTC 6 cut(s) 680, 1986, 3493, 4285, 4844, 5383
PmaCI CACGTG 1 cut(s) 1106
PmlI CACGTG 1 cut(s) 1106
PpsI GAGTC 6 cut(s) 680, 1986, 3493, 4285, 4844, 5383
Ppu21I YACGTR 3 cut(s) 523, 1106, 2687
PpuMI RGGWCCY 1 cut(s) 2834
PscI ACATGT 3 cut(s) 3187, 3331, 4050
PsiI TTATAA 2 cut(s) 147, 573
Psp124BI GAGCTC 1 cut(s) 2745
Psp1406I AACGTT 1 cut(s) 5434
Psp5II RGGWCCY 1 cut(s) 2834
Psp6I CCWGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
PspCI CACGTG 1 cut(s) 1106
PspGI CCWGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
PspN4I GGNNCC 8 cut(s) 846, 1248, 1518, 1861, 1872, 2150, 2451, 3596
PspPI GGNCC 4 cut(s) 340, 2038, 2149, 2834
PspPPI RGGWCCY 1 cut(s) 2834
PstI CTGCAG 4 cut(s) 1163, 2644, 2695, 3835
PstNI CAGNNNCTG 4 cut(s) 68, 986, 1592, 4988
PsuI RGATCY 6 cut(s) 983, 1547, 1870, 2779, 3384, 4711
PsyI GACNNNGTC 1 cut(s) 1995
PvuII CAGCTG 1 cut(s) 1133
RseI CAYNNNNRTG 5 cut(s) 1945, 3332, 4314, 4779, 4785
SacI GAGCTC 1 cut(s) 2745
SacII CCGCGG 1 cut(s) 5280
SalI GTCGAC 1 cut(s) 3458
Sau96I GGNCC 4 cut(s) 340, 2038, 2149, 2834
ScaI AGTACT 3 cut(s) 1157, 1209, 4528
SchI GAGTC 6 cut(s) 681, 1986, 3494, 4285, 4845, 5384
ScrFI CCNGG 8 cut(s) 19, 852, 912, 1365, 1521, 1783, 4057, 4095
SduI GDGCHC 9 cut(s) 515, 2454, 2745, 2812, 3878, 4524, 4725, 5020, 5322
SfcI CTRYAG 6 cut(s) 1159, 1511, 2640, 2691, 3831, 5313
Sfr303I CCGCGG 1 cut(s) 5280
SgrBI CCGCGG 1 cut(s) 5280
SinI GGWCC 3 cut(s) 340, 2038, 2834
SmiMI CAYNNNNRTG 5 cut(s) 1945, 3332, 4314, 4779, 4785
SmlI CTYRAG 7 cut(s) 2846, 3527, 3734, 3803, 3902, 4061, 5090
SmoI CTYRAG 7 cut(s) 2846, 3527, 3734, 3803, 3902, 4061, 5090
SphI GCATGC 3 cut(s) 3911, 4659, 5345
SspI AATATT 1 cut(s) 82
SstI GAGCTC 1 cut(s) 2745
StyD4I CCNGG 8 cut(s) 17, 850, 910, 1363, 1519, 1781, 4055, 4093
StyI CCWWGG 6 cut(s) 1169, 1250, 1555, 3681, 4443, 5025
TaiI ACGT 7 cut(s) 245, 525, 1108, 2689, 5212, 5437, 5485
TaqI TCGA 7 cut(s) 515, 541, 2043, 2400, 2812, 3459, 4513
TatI WGTACW 5 cut(s) 1155, 1207, 4526, 4624, 5297
TauI GCSGC 3 cut(s) 286, 493, 5474
TseFI GTSAC 6 cut(s) 484, 1067, 1453, 1712, 1718, 5165
TseI GCWGC 8 cut(s) 2693, 3754, 3830, 4148, 4359, 4888, 4907, 5003
Tsp45I GTSAC 6 cut(s) 484, 1067, 1453, 1712, 1718, 5165
TspGWI ACGGA 1 cut(s) 1985
Tth111I GACNNNGTC 1 cut(s) 1995
Van91I CCANNNNNTGG 2 cut(s) 1146, 3246
Vha464I CTTAAG 2 cut(s) 3734, 5090
VpaK11BI GGWCC 3 cut(s) 340, 2038, 2834
XbaI TCTAGA 2 cut(s) 2576, 5077
XcmI CCANNNNNNNNNTGG 1 cut(s) 4372
XmiI GTMKAC 3 cut(s) 3459, 3577, 4306
XmnI GAANNNNTTC 4 cut(s) 2262, 3972, 4507, 5459
ZrmI AGTACT 3 cut(s) 1157, 1209, 4528
Zsp2I ATGCAT 1 cut(s) 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.