RLG00000003906

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004720
Physical Location & Seq
Reverse (-)
54132666 .. 54146382
13717 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003906

Sequence Viewer

Length: 5538 bp
ATGGGTAAAGGAAGACCTAGAGCTGTGGAGAAGGGGGTGGTGGGGCCCAATTTGAGTGCTTCATCGTCTTCTTCGTCTGGGTCGTCGTTGACCATACCATCTGCACCCGTGTATTACCCTAGTGAGGATGAGTTTAGGGATCCTTTGGAGTATATATGTAAGATCAGGGCTGAGGCTGAGCCCTATGGGATTTGTAGGATAGTTCCGCCGAGTAGCTGGAAGCCCCCATTTGCTTTGGACTTGGATTCGTTTACATTTCCGACGAAGACGCAGGCCATTCACCAGTTGCAGGTGAGGCCTGCGTCGTGTGATTCAAAGACTTTTGAGTTGGAGTATAATAGGTTTTTGGAGGATCATTGTGGGAAGAGGTTGAGGAGGAAGGTGGTTTTTGAAGGGGAGGAGTTGGATTTGTGTAAGCTGTTCAATGCTGCAAAGAGGTATGGGGGGTATGATAAGGTTGTGAAGGAGAAGAAGTGGGGGGAGGTTGTGAGGTTTGTGAGGCCGGCAAGGAAGGTTTCTGAGTGTTCGAAGCATGTGTTGCATCAGTTGTATCTGGAGCATTTGTTTGAGTATGAGGAGTATTATAATAAGCTGAATAAAGAAGGGGGTAGGGGTTGTAAGAGGGGTTTGCAGGAGGAGAAGAAGGGTGAACATAATGCTGAATGTTCTAGCTCCAAAAGGAGGCGAACGAATAATAACGATGGTGAGAGGGTTAGGATTCGAAAGGTAAAGAAGAAAGAGGAGGAACATGATCAGATATGTGAGCAATGCAGAAGTGGGTTGCATGGGGAAGTGATGCTTTTGTGTGATAGGTGTGATAAGGGGTGGCACATTTACTGTCTCTCTCCACCGTTGAAACAGATCCCGTCAGGGAATTGGTATTGCCTAGATTGCTTGAATTCTGATGAAGATTGTTTTGGATTTGTGCCTGGAAAGCGTTTCTCACTGGAAGCTTTTAGGCGCGTGGCCGATCGGGCCAAGAAGAAATGGTTTGGGTCAGGACCTGCGTCTCGGGTGCAAATAGAGAAAAAGTTCTGGGAAATTGTGGAGGGTTCCATCGGTGAGGTTGAAGTTATGTATGGCAGCGACCTGGATACTTCCATTTATGGGAGCGGTTTTCCGCGTGTAAATGACCAGAGACAAGAGTCAGTTGAGGCCAAGATATGGGATGAATATTGCGGTAGCCCATGGAATCTCAATAACTTGCCCAAGTTGAAAGGATCAGTTCTTAGAGCTGTTCATAATAACATTACTGGTGTTATGGTGCCTTGGCTCTATATTGGGATGCTGTTTTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTATTCAATGAATTATCATCACTGGGGAGAGCCCAAATGCTGGTACAGCGTCCCTGGTAGTGAAGCCAGTGCCTTTGAAAAGGTGATGCGTAACAGTCTTCCTGATCTTTTTGATGCACAACCTGATTTGCTCTTTCAGCTTGTCACTATGTTAAATCCATCTGTTTTGCAAGAGAATGGAGTTCCTGTATACAGCGTCCTACAGGAGCCTGGAAATTTTGTCATCACCTTCCCCAGGTCTTACCATGGAGGTTTTAATCTTGGTTTGAATTGTGCAGAGGCTGTCAATTTTGCCCCTGCTGACTGGTTACCTCATGGTGGGTTTGGAGCAGGGCTCTATCAGCTTTATCATAAAACTGCAGTTCTGTCTCACGAGGAGCTCGTTTGTGTGCTAGCCAAGAGTGACTGTGACAGCAGAGTATCACCTTATTTGAAGGAAGAATTGATAAGAATATATAACAAAGAGAAAACCTGGAGAGAGCGCCTTTGGAAAAAAGGAATCACCAAATCATCTCTTATGTCTTCTCGGAAGTTCCCTGAATATGTTGGTACTGAGGAGGATCCCACGTGCATTATATGCCAGCAATATCTTTACCTCTCTGGTGTGGTTTGTCGTTGTCGACCATCCACATTTGTATGTCTGGAGCACTCGGAACGTCTTTGTGAATGCAAATCCAGCAGACTCCGTCTTCATTATCGCCATACACTTGCAGAATTGCATGACTTGGTGCTTGCAATGGGTAAACATTGTGGTGAGGAGACAACACAAAGTAGAACAAAAAAAAGACAACTCCAATGCTCCAATGAACCTGCTTTAACAAAAAAGGTCAAGGGTGGTCATGTTAATTTTGCTCAACTTGCGGACAAATGGCTTTTGCGCTCATCCAAGATTTTCCATGGTCGGTTTTCCAGAGAAGACTATGTTACTGTATTGAAAGAAGCTGAACAATTTGTTTGGGCTGGTTCAGAGATGAATAATGTTCGGGAAATGGCAAATAATTTGAAAGAAGCTCGGAAATGGGCAGAGGGTGTGAGAAAGTCTGTATCTAAAATTGAAAGTTGGTCATCTCATTGCGACAAAGATATAGAGAAGATTCACTTGGAACGTATCAATGAGTTGCTGAGTTTTGATTTGTTGCCCTGTGATGAGCCTGGGCATCTTATTTTGAAGGATTATGCTGAAAAGGCAAGGATACTGATAGAGGAAATCAATACTGCTATGTCTTCATGCCCAAAAATCCCTGAGTTGGAATTGTTGTACAACAGAGTTTGTGAGTTCCCAATTTATGTAAAAGAAAGTGAGAGGTTACAACACAAAATTTCATCTGCAAAGGTTTGGATAGAAGGCATAACAAAATGCATCTCGGAGAAACAGCCTGCTGCAATTGAGCTTGATGTTCTTTACAAGTTGAAATTGGAGATCCCAGAAGTCGAAGTTCAACTCCCACAGATAGAAATGCTTTCTGATATAGTAGGGAAAGCGGAATCATGTCGAGCTCAATGTGTTGAAATTCTGAAAGGTCCAATTAGCCTCAAGGATATTGAGGTGCTTCTTCTAGAATGGGATGCTTTTACTGTCAACATACCAGAATTGAAGCTTTTGAGGCAGTACCACACTGATGCTGTTTCTTGGCATGCTCTCTTTGAAGCTGTTCTTATGAAAGTTCATGAAAGAGAGGATCAACATAATGTAGTTGATGAGTTAGAGCACATTCTAACGGATGGGGCAACTTTGAAAATCCAAGTTGATCAGATGCCTTTGGTTGAGTTTGAGTTGAAAAAGGCTCGGTGCAGGGAAAGGGCTTTGAGGACACGAGAATCTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGTTGGATGCACAAGGTTTACATATTGAGGGAGAACAAATATATGTTGATATGTCCAAAGTACTTGATGCTGCCATCCAATGGGAGGAAAGAGCAAAATATATTCTTGCACATGGAGCTCAAATTTCTGACTTTGAGGATGTTCTTAGGAGTTCGGAAAACATACATGTGACTTTACCTTCTCTCCTTGATGTCAAGGAGGCACTATCAAAGGCTATGGTCTGGTTGAGTAGATCTGAGCCGTTTCTTGTGCAATGTTCTTCGTTGGAATCTGGCTCAAGTTCTTTGCTGAAAGTTGACACCTTGAAGGAGTTGATTTCTGAGTCGAAGAATCTTAAAGTATCTATGAAAGAAATTAAGATTCTTGAGACTGTTTTGATGAACTGTGAGGAATGGGAGCATGATGCTTGCTCTCTACTGCAAGATTCAAGATGCCTATTGGATGAGGGGATAAGTGAGGGACTTATTTCCAAGATTGAAAATGTACTTGCAAGAATAGGATCCATGGAAAACACTGGTTTATCTCTTACTTTTGACTTTGTTGAGCTTGCAAAACTCAAGGATGCTTGTTCTCTGCTGCAATGGTGTAAGAAGGCTATCTCTTTCTGCTCTGCTGCTCCTACTCTTGAGGATGTTGAGAGTTTGATGAGTGATGCAGAGACTTCATGTTGTACAAATGCCTCTGGTGCACTCTGTGACTTGTTGTTTGAAGGAGTCAAATGGCTTAGGCAAGCTACAAAGATTATTTCTGCATCTAGCAATTCTACAAGATGCAAGTTGAGTGAAGCTGAAGAAGTTCTTATTGATTCCCAGAGTATTAGTATCTCCTTCCCTCTGACAGTCACTCAAATTGAAACTGCCATTGAGAAACATAAGTCTTGGCTAGAGCAAGTGCATCAATTTTTCAGCCTAAGGCTGGGGGAACGATCTTGGTCTGTTGTATTACAGCTAAAGGAGCTTGGGATTGCTGGTGCTTTCTATTGCCCAGAACTAGATTCAATATTATCTGAAGTTGAAAGGGTTCAGAAGTGGAAGCGACAATGCATGGATATTTTCAGGATTGTAGAGGAGAATTCACTGCTTGGTTCTCTAGAAAAGATACGACAGAATCTGGATAGATCTATGCATATATATGACAAAGCACATGGTTTGAAAGAGGAAGGTTCTTTTGCGTGCTGCTCCATTGGTTCTCTGGATCAGGAGTTTCTTACCTGTTCTTTGTGCAAGGAATGCTACCATTTGCGGTGCTTAGGATCTTTGTCAGTAGATGGGAAACAGTCAGCATATGTGTGCCTGTGTTGCCAGTATCTAGTATCTGGAACTTTGCAAAATGGAGGAAGTCTTCGGGGATTTGGAGGAGTTCGTCCAGCACTGCAAAAGATTACTGAACTCTTATCTGATGAAGAGGATTTCTGTGTTTGTATGGAAGAAAGAGAGATGTTGAAAGAGGTTTTGAAGCAAGCTCGGGTATGCAAGACACGCTTGGAAGGAATAGTGGATTTTGCATTAGCTTATTCGGACAAAGATCTCTGTGTCCTCTTCAAAAAACTAGGCACTGCTTTAAAGGCCATAGAGCTGGGAGGTTTTTATGACGATGAAGGTTATTGCAACCTTACATTGGCAATATCAAGATACTCATGGAAAGTCAAAGTTAATAGATTACTCGAGGGTTCACAAAAGCCCACAATCCAACAGATTCAACAACATTTAAAAGAGAGAGTGGCCGTGAACATTCCACCTGAAGATTACTTTAAGCAAAAACTTACAGAACTGAAGTGCAGTGGCTTGCAGTGGGCAGATAAGGCTAAGAAGGTTGCTGCTGATTCTGGGGCTCTCCCTTTGGAGAATGTTTTTGAACTTATTTCTGAGGGTGAAAACTTACCTGTTCTTGTAGAGAAGGAACTTAAGCTATTGAAAGATCGGAGTATGCTTTATTGCATATGTAGAAAGCCTTCTGATCAGAGAGCAATGATTGCATGTGACAAGTGTGATGAGTGGTATCACTTTGGTTGTGTGAAATTACGTTCCACACCAAAGGTTTACATTTGCGCTGCATGTGAGCCTCTAGCAGAAACATTGTCTTCATCAGCAGTAGTTGATAGTGAGAGATGTGCTGATGCCAAATTTGTAGAACCAAAAACACCCTCTCCTAAGCACACAAAGCCCAGGATGAGCCCAAACAAAGATGAGTTTGTTGCAACACAAAAGATGGTTGCAAGAACAGATGACGCCAATGTTTTTAGGTCATCTAGTGGGATAGACTGTTTATGGTGGCGAAATCGGAAGCCTTTCAGAAGGGCGGCAAAAAAACGAGCTGAGCTTGATAGTCTGTCTCTATTTTCTCATTTGCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1846

Amino Acids

210.03

Weight (kDa)

6.29

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 36 - 69 1.8e-16 jmjN domain
ARID PF01388 107 - 192 1.5e-10 ARID/BRIGHT DNA binding domain
PHD PF00628 254 - 298 8.3e-09 PHD-finger
JmjC PF02373 424 - 540 3.7e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 633 - 685 7.4e-14 C5HC2 zinc finger
PLU-1 PF08429 995 - 1192 2.1e-16 PLU-1-like protein
PLU-1 PF08429 1151 - 1412 9.6e-17 PLU-1-like protein
PHD PF00628 1706 - 1746 6.5e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 3 cut(s) 280, 1012, 2146
AccB1I GGYRCC 1 cut(s) 1264
AccB7I CCANNNNNTGG 3 cut(s) 1164, 1368, 3261
AccBSI CCGCTC 1 cut(s) 1113
AccI GTMKAC 2 cut(s) 1518, 1948
AccII CGCG 2 cut(s) 963, 1123
AciI CCGC 8 cut(s) 206, 1113, 1121, 1179, 2189, 2810, 4423, 5480
AcoI YGGCCR 2 cut(s) 966, 4902
AcsI RAATTY 7 cut(s) 898, 1543, 2646, 2838, 3303, 4252, 5303
AcuI CTGAAG 4 cut(s) 3990, 4209, 4941, 4973
AcvI CACGTG 1 cut(s) 1896
AcyI GRCGYC 1 cut(s) 5409
AdeI CACNNNGTG 1 cut(s) 3875
AfaI GTAC 7 cut(s) 1373, 1879, 2588, 2939, 3243, 3666, 3853
AflII CTTAAG 1 cut(s) 5084
AflIII ACRYGT 1 cut(s) 3346
AjnI CCWGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
AjuI GAANNNNNNNTTGG 4 cut(s) 900, 932, 2411, 2443
Alw21I GWGCWC 6 cut(s) 1710, 1977, 2827, 3039, 3301, 3871
Alw26I GTCTC 9 cut(s) 845, 1014, 1132, 1701, 2081, 3162, 3542, 3833, 5517
Alw44I GTGCAC 1 cut(s) 3867
AlwNI CAGNNNCTG 3 cut(s) 1004, 1610, 4291
Ama87I CYCGRG 3 cut(s) 1011, 4644, 4844
AoxI GGCC 9 cut(s) 44, 273, 296, 500, 966, 975, 1155, 4746, 4902
ApaI GGGCCC 1 cut(s) 48
ApaLI GTGCAC 1 cut(s) 3867
ApeKI GCWGC 9 cut(s) 428, 1083, 2708, 3251, 3757, 3794, 4356, 4997, 5231
ApoI RAATTY 7 cut(s) 898, 1543, 2646, 2838, 3303, 4252, 5303
ArsI GACNNNNNNTTYG 2 cut(s) 3869, 3901
Asp700I GAANNNNTTC 6 cut(s) 1031, 2979, 3975, 4200, 5131, 5468
AspLEI GCGC 4 cut(s) 963, 1812, 2208, 5231
AspS9I GGNCC 5 cut(s) 44, 45, 975, 1001, 2849
AsuII TTCGAA 2 cut(s) 527, 721
AsuNHI GCTAGC 1 cut(s) 1720
AvaI CYCGRG 3 cut(s) 1011, 4644, 4844
AvaII GGWCC 2 cut(s) 1001, 2849
AxyI CCTNAGG 1 cut(s) 4091
BaeGI GKGCMC 2 cut(s) 48, 3871
BamHI GGATCC 3 cut(s) 139, 1888, 3680
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 9 cut(s) 48, 183, 1362, 1665, 1710, 2827, 3301, 5014, 5357
BarI GAAGNNNNNNTAC 2 cut(s) 5116, 5148
BauI CACGAG 2 cut(s) 1700, 3141
BbrPI CACGTG 1 cut(s) 1896
Bbv12I GWGCWC 6 cut(s) 1710, 1977, 2827, 3039, 3301, 3871
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 9 cut(s) 415, 1095, 2695, 3238, 3744, 3781, 4343, 4984, 5218
BccI CCATC 9 cut(s) 106, 695, 1064, 1495, 1960, 3044, 3263, 4442, 5383
BceAI ACGGC 2 cut(s) 3406, 4889
BciT130I CCWGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
BciVI GTATCC 2 cut(s) 1087, 2514
BclI TGATCA 3 cut(s) 751, 3076, 5137
BcoDI GTCTC 9 cut(s) 845, 1014, 1132, 1701, 2081, 3162, 3542, 3833, 5517
BfmI CTRYAG 2 cut(s) 1529, 1686
BfoI RGCGCY 1 cut(s) 1813
BfrI CTTAAG 1 cut(s) 5084
BfuAI ACCTGC 3 cut(s) 280, 1012, 2146
BfuI GTATCC 2 cut(s) 1087, 2514
BglI GCCNNNNNGGC 1 cut(s) 974
BglII AGATCT 3 cut(s) 3413, 4298, 4705
BlpI GCTNAGC 2 cut(s) 177, 5496
BmcAI AGTACT 1 cut(s) 3243
Bme1390I CCNGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
Bme18I GGWCC 2 cut(s) 1001, 2849
BmeT110I CYCGRG 3 cut(s) 1011, 4644, 4844
BmgT120I GGNCC 5 cut(s) 44, 45, 975, 1001, 2849
BmiI GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 1890, 3682
BmrFI CCNGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
BmrI ACTGGG 1 cut(s) 1360
BmtI GCTAGC 1 cut(s) 1724
BmuI ACTGGG 1 cut(s) 1360
BoxI GACNNNNGTC 2 cut(s) 1006, 1144
BplI GAGNNNNNCTC 2 cut(s) 1647, 1679
BpmI CTGGAG 3 cut(s) 575, 1822, 1991
Bpu10I CCTNAGC 4 cut(s) 171, 3905, 4429, 5331
Bpu1102I GCTNAGC 2 cut(s) 177, 5496
Bpu14I TTCGAA 2 cut(s) 527, 721
BpuEI CTTGAG 5 cut(s) 2846, 3442, 3566, 3722, 3827
BsaAI YACGTR 1 cut(s) 1896
BsaHI GRCGYC 1 cut(s) 5409
BsaJI CCNNGG 9 cut(s) 1187, 1268, 1381, 1562, 1573, 2224, 2480, 3684, 5345
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3700, 4483
Bse21I CCTNAGG 1 cut(s) 4091
Bse3DI GCAATG 6 cut(s) 773, 2070, 2398, 3440, 3767, 5154
BseBI CCWGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
BseDI CCNNGG 9 cut(s) 1187, 1268, 1381, 1562, 1573, 2224, 2480, 3684, 5345
BseMI GCAATG 6 cut(s) 773, 2070, 2398, 3440, 3767, 5154
BseNI ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3700, 4483
BseSI GKGCMC 2 cut(s) 48, 3871
BseXI GCAGC 9 cut(s) 415, 1095, 2695, 3238, 3744, 3781, 4343, 4984, 5218
BseYI CCCAGC 2 cut(s) 4096, 4756
BsgI GTGCAG 4 cut(s) 87, 1623, 3139, 4978
Bsh1236I CGCG 2 cut(s) 963, 1123
Bsh1285I CGRYCG 1 cut(s) 973
BshFI GGCC 9 cut(s) 46, 275, 298, 502, 968, 977, 1157, 4748, 4904
BshNI GGYRCC 1 cut(s) 1264
BsiEI CGRYCG 1 cut(s) 973
BsiHKAI GWGCWC 6 cut(s) 1710, 1977, 2827, 3039, 3301, 3871
BsiHKCI CYCGRG 3 cut(s) 1011, 4644, 4844
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 1364, 3654
BsmAI GTCTC 9 cut(s) 845, 1014, 1132, 1701, 2081, 3162, 3542, 3833, 5517
BsmBI CGTCTC 1 cut(s) 1014
BsmFI GGGAC 2 cut(s) 1364, 3654
BsmI GAATGC 2 cut(s) 2000, 4415
BsnI GGCC 9 cut(s) 46, 275, 298, 502, 968, 977, 1157, 4748, 4904
BsoBI CYCGRG 3 cut(s) 1011, 4644, 4844
Bsp119I TTCGAA 2 cut(s) 527, 721
Bsp120I GGGCCC 1 cut(s) 44
Bsp1407I TGTACA 2 cut(s) 2586, 3851
Bsp1720I GCTNAGC 2 cut(s) 177, 5496
Bsp19I CCATGG 4 cut(s) 1187, 1573, 2224, 3684
BspACI CCGC 8 cut(s) 206, 1113, 1121, 1179, 2189, 2810, 4423, 5480
BspANI GGCC 9 cut(s) 46, 275, 298, 502, 968, 977, 1157, 4748, 4904
BspFNI CGCG 2 cut(s) 963, 1123
BspHI TCATGA 1 cut(s) 2995
BspLI GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 1890, 3682
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 3 cut(s) 280, 1012, 2146
BspOI GCTAGC 1 cut(s) 1724
BspT104I TTCGAA 2 cut(s) 527, 721
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 5084
BsrBI CCGCTC 1 cut(s) 1113
BsrDI GCAATG 6 cut(s) 773, 2070, 2398, 3440, 3767, 5154
BsrFI RCCGGY 1 cut(s) 502
BsrGI TGTACA 2 cut(s) 2586, 3851
BsrI ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3700, 4483
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 9 cut(s) 1187, 1268, 1381, 1562, 1573, 2224, 2480, 3684, 5345
BssNAI GTATAC 1 cut(s) 1519
BssNI GRCGYC 1 cut(s) 5409
BssSI CACGAG 2 cut(s) 1700, 3141
BssT1I CCWWGG 5 cut(s) 1187, 1268, 1573, 2224, 3684
Bst1107I GTATAC 1 cut(s) 1519
Bst2BI CACGAG 2 cut(s) 1700, 3141
Bst2UI CCWGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
Bst6I CTCTTC 3 cut(s) 359, 4578, 4724
BstACI GRCGYC 1 cut(s) 5409
BstAFI CTTAAG 1 cut(s) 5084
BstAPI GCANNNNNTGC 4 cut(s) 538, 1905, 4410, 5153
BstAUI TGTACA 2 cut(s) 2586, 3851
BstBAI YACGTR 1 cut(s) 1896
BstBI TTCGAA 2 cut(s) 527, 721
BstDSI CCRYGG 4 cut(s) 1187, 1573, 2224, 3684
BstEII GGTNACC 1 cut(s) 1635
BstENI CCTNNNNNAGG 1 cut(s) 1561
BstFNI CGCG 2 cut(s) 963, 1123
BstH2I RGCGCY 1 cut(s) 1813
BstHHI GCGC 4 cut(s) 963, 1812, 2208, 5231
BstMAI GTCTC 9 cut(s) 845, 1014, 1132, 1701, 2081, 3162, 3542, 3833, 5517
BstMCI CGRYCG 1 cut(s) 973
BstNI CCWGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
BstNSI RCATGY 5 cut(s) 536, 2966, 3350, 5160, 5238
BstPAI GACNNNNGTC 2 cut(s) 1006, 1144
BstPI GGTNACC 1 cut(s) 1635
BstSCI CCNGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
BstSFI CTRYAG 2 cut(s) 1529, 1686
BstSLI GKGCMC 2 cut(s) 48, 3871
BstUI CGCG 2 cut(s) 963, 1123
BstV1I GCAGC 9 cut(s) 415, 1095, 2695, 3238, 3744, 3781, 4343, 4984, 5218
BstX2I RGATCY 9 cut(s) 139, 861, 1888, 2748, 3413, 3680, 4298, 4433, 4705
BstXI CCANNNNNNTGG 1 cut(s) 4756
BstYI RGATCY 9 cut(s) 139, 861, 1888, 2748, 3413, 3680, 4298, 4433, 4705
BstZ17I GTATAC 1 cut(s) 1519
Bsu36I CCTNAGG 1 cut(s) 4091
BsuI GTATCC 2 cut(s) 1087, 2514
BsuRI GGCC 9 cut(s) 46, 275, 298, 502, 968, 977, 1157, 4748, 4904
BtgI CCRYGG 4 cut(s) 1187, 1573, 2224, 3684
BtsI GCAGTG 5 cut(s) 4256, 4550, 4734, 4966, 4976
BveI ACCTGC 3 cut(s) 280, 1012, 2146
CaiI CAGNNNCTG 3 cut(s) 1004, 1610, 4291
CciI TCATGA 1 cut(s) 2995
CfoI GCGC 4 cut(s) 963, 1812, 2208, 5231
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 5 cut(s) 44, 45, 975, 1001, 2849
CseI GACGC 6 cut(s) 277, 291, 996, 1366, 1513, 5417
Csp6I GTAC 7 cut(s) 1372, 1878, 2587, 2938, 3242, 3665, 3852
CviQI GTAC 7 cut(s) 1372, 1878, 2587, 2938, 3242, 3665, 3852
DraI TTTAAA 2 cut(s) 4743, 4890
DraIII CACNNNGTG 1 cut(s) 3875
EaeI YGGCCR 2 cut(s) 966, 4902
Eam1104I CTCTTC 3 cut(s) 359, 4578, 4724
EarI CTCTTC 3 cut(s) 359, 4578, 4724
EciI GGCGGA 1 cut(s) 195
Ecl136II GAGCTC 3 cut(s) 1708, 2825, 3299
Eco130I CCWWGG 5 cut(s) 1187, 1268, 1573, 2224, 3684
Eco147I AGGCCT 1 cut(s) 298
Eco24I GRGCYC 9 cut(s) 48, 183, 1362, 1665, 1710, 2827, 3301, 5014, 5357
Eco47I GGWCC 2 cut(s) 1001, 2849
Eco53kI GAGCTC 3 cut(s) 1708, 2825, 3299
Eco57I CTGAAG 4 cut(s) 3990, 4209, 4941, 4973
Eco72I CACGTG 1 cut(s) 1896
Eco81I CCTNAGG 1 cut(s) 4091
Eco88I CYCGRG 3 cut(s) 1011, 4644, 4844
Eco91I GGTNACC 1 cut(s) 1635
EcoICRI GAGCTC 3 cut(s) 1708, 2825, 3299
EcoNI CCTNNNNNAGG 1 cut(s) 1561
EcoO109I RGGNCCY 2 cut(s) 44, 1001
EcoO65I GGTNACC 1 cut(s) 1635
EcoRI GAATTC 2 cut(s) 898, 4252
EcoRII CCWGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
EcoT14I CCWWGG 5 cut(s) 1187, 1268, 1573, 2224, 3684
EcoT22I ATGCAT 3 cut(s) 2690, 4226, 4308
EcoT38I GRGCYC 9 cut(s) 48, 183, 1362, 1665, 1710, 2827, 3301, 5014, 5357
ErhI CCWWGG 5 cut(s) 1187, 1268, 1573, 2224, 3684
Esp3I CGTCTC 1 cut(s) 1014
FaqI GGGAC 2 cut(s) 1364, 3654
FauNDI CATATG 2 cut(s) 4465, 5120
FbaI TGATCA 3 cut(s) 751, 3076, 5137
FblI GTMKAC 2 cut(s) 1518, 1948
FriOI GRGCYC 9 cut(s) 48, 183, 1362, 1665, 1710, 2827, 3301, 5014, 5357
GlaI GCGC 4 cut(s) 962, 1811, 2207, 5230
GsaI CCCAGC 2 cut(s) 4100, 4760
GsuI CTGGAG 3 cut(s) 575, 1822, 1991
HaeII RGCGCY 1 cut(s) 1813
HaeIII GGCC 9 cut(s) 46, 275, 298, 502, 968, 977, 1157, 4748, 4904
HapII CCGG 1 cut(s) 503
HgaI GACGC 6 cut(s) 277, 291, 996, 1366, 1513, 5417
HhaI GCGC 4 cut(s) 963, 1812, 2208, 5231
Hin1I GRCGYC 1 cut(s) 5409
Hin6I GCGC 4 cut(s) 961, 1810, 2206, 5229
HinP1I GCGC 4 cut(s) 961, 1810, 2206, 5229
HincII GTYRAC 4 cut(s) 90, 1949, 2908, 3478
HindII GTYRAC 4 cut(s) 90, 1949, 2908, 3478
HindIII AAGCTT 2 cut(s) 951, 2924
HpaII CCGG 1 cut(s) 503
Hpy99I CGWCG 3 cut(s) 88, 265, 307
HpyCH4IV ACGT 4 cut(s) 1895, 1984, 2434, 5203
HpySE526I ACGT 4 cut(s) 1895, 1984, 2434, 5203
Hsp92I GRCGYC 1 cut(s) 5409
HspAI GCGC 4 cut(s) 961, 1810, 2206, 5229
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Ksp22I TGATCA 3 cut(s) 751, 3076, 5137
Lsp1109I GCAGC 9 cut(s) 415, 1095, 2695, 3238, 3744, 3781, 4343, 4984, 5218
MaeII ACGT 4 cut(s) 1895, 1984, 2434, 5203
MbiI CCGCTC 1 cut(s) 1113
MfeI CAATTG 1 cut(s) 2712
MflI RGATCY 9 cut(s) 139, 861, 1888, 2748, 3413, 3680, 4298, 4433, 4705
MlyI GAGTC 4 cut(s) 1154, 2004, 3512, 3903
MmeI TCCRAC 7 cut(s) 284, 309, 384, 2556, 3165, 3426, 4894
Mph1103I ATGCAT 3 cut(s) 2690, 4226, 4308
MroNI GCCGGC 1 cut(s) 502
MroXI GAANNNNTTC 6 cut(s) 1031, 2979, 3975, 4200, 5131, 5468
MslI CAYNNNNRTG 6 cut(s) 1963, 2079, 2946, 3347, 4311, 4468
MspCI CTTAAG 1 cut(s) 5084
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
MunI CAATTG 1 cut(s) 2712
Mva1269I GAATGC 2 cut(s) 2000, 4415
MvaI CCWGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
MvnI CGCG 2 cut(s) 963, 1123
NaeI GCCGGC 1 cut(s) 504
NcoI CCATGG 4 cut(s) 1187, 1573, 2224, 3684
NdeI CATATG 2 cut(s) 4465, 5120
NgoMIV GCCGGC 1 cut(s) 502
NheI GCTAGC 1 cut(s) 1720
NlaIV GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 1890, 3682
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 7 cut(s) 1471, 1730, 1736, 3349, 3875, 4021, 5159
NsiI ATGCAT 3 cut(s) 2690, 4226, 4308
NspI RCATGY 5 cut(s) 536, 2966, 3350, 5160, 5238
NspV TTCGAA 2 cut(s) 527, 721
PaeI GCATGC 1 cut(s) 2966
PaeR7I CTCGAG 1 cut(s) 4844
PagI TCATGA 1 cut(s) 2995
PaqCI CACCTGC 1 cut(s) 280
PceI AGGCCT 1 cut(s) 298
PciI ACATGT 1 cut(s) 3346
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 80, 1707
PctI GAATGC 2 cut(s) 2000, 4415
PdiI GCCGGC 1 cut(s) 504
PdmI GAANNNNTTC 6 cut(s) 1031, 2979, 3975, 4200, 5131, 5468
PflFI GACNNNGTC 1 cut(s) 2013
PflMI CCANNNNNTGG 3 cut(s) 1164, 1368, 3261
Ple19I CGATCG 1 cut(s) 973
PleI GAGTC 4 cut(s) 1153, 2004, 3511, 3902
PmaCI CACGTG 1 cut(s) 1896
PmlI CACGTG 1 cut(s) 1896
PpsI GAGTC 4 cut(s) 1153, 2004, 3511, 3902
Ppu21I YACGTR 1 cut(s) 1896
PpuMI RGGWCCY 1 cut(s) 1001
PscI ACATGT 1 cut(s) 3346
PshAI GACNNNNGTC 2 cut(s) 1006, 1144
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 3 cut(s) 1710, 2827, 3301
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
PspCI CACGTG 1 cut(s) 1896
PspEI GGTNACC 1 cut(s) 1635
PspFI CCCAGC 2 cut(s) 4096, 4756
PspGI CCWGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
PspN4I GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 1890, 3682
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 5 cut(s) 44, 45, 975, 1001, 2849
PspPPI RGGWCCY 1 cut(s) 1001
PspXI VCTCGAGB 1 cut(s) 4844
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 3 cut(s) 1004, 1610, 4291
PsuI RGATCY 9 cut(s) 139, 861, 1888, 2748, 3413, 3680, 4298, 4433, 4705
PsyI GACNNNGTC 1 cut(s) 2013
PvuI CGATCG 1 cut(s) 973
RsaI GTAC 7 cut(s) 1373, 1879, 2588, 2939, 3243, 3666, 3853
RsaNI GTAC 7 cut(s) 1372, 1878, 2587, 2938, 3242, 3665, 3852
RseI CAYNNNNRTG 6 cut(s) 1963, 2079, 2946, 3347, 4311, 4468
SacI GAGCTC 3 cut(s) 1710, 2827, 3301
SalI GTCGAC 1 cut(s) 1947
Sau96I GGNCC 5 cut(s) 44, 45, 975, 1001, 2849
ScaI AGTACT 1 cut(s) 3243
SchI GAGTC 4 cut(s) 1154, 2004, 3512, 3903
ScrFI CCNGG 8 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2481, 5347
SfcI CTRYAG 2 cut(s) 1529, 1686
SfiI GGCCNNNNNGGCC 1 cut(s) 974
Sfr274I CTCGAG 1 cut(s) 4844
SfuI TTCGAA 2 cut(s) 527, 721
SinI GGWCC 2 cut(s) 1001, 2849
SlaI CTCGAG 1 cut(s) 4844
SmiMI CAYNNNNRTG 6 cut(s) 1963, 2079, 2946, 3347, 4311, 4468
SmlI CTYRAG 7 cut(s) 2861, 3457, 3545, 3737, 3806, 4844, 5084
SmoI CTYRAG 7 cut(s) 2861, 3457, 3545, 3737, 3806, 4844, 5084
SphI GCATGC 1 cut(s) 2966
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 8 cut(s) 206, 1113, 1121, 1179, 2189, 2810, 4423, 5480
SspI AATATT 2 cut(s) 1175, 4182
SstI GAGCTC 3 cut(s) 1710, 2827, 3301
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 8 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2479, 5345
StyI CCWWGG 5 cut(s) 1187, 1268, 1573, 2224, 3684
TaiI ACGT 4 cut(s) 1898, 1987, 2437, 5206
TaqI TCGA 7 cut(s) 527, 721, 1948, 2760, 2821, 3506, 4845
TatI WGTACW 4 cut(s) 2586, 3241, 3664, 3851
TauI GCSGC 1 cut(s) 5483
TseFI GTSAC 7 cut(s) 1471, 1730, 1736, 3349, 3875, 4021, 5159
TseI GCWGC 9 cut(s) 428, 1083, 2708, 3251, 3757, 3794, 4356, 4997, 5231
Tsp45I GTSAC 7 cut(s) 1471, 1730, 1736, 3349, 3875, 4021, 5159
TspGWI ACGGA 2 cut(s) 2003, 3062
Tth111I GACNNNGTC 1 cut(s) 2013
Van91I CCANNNNNTGG 3 cut(s) 1164, 1368, 3261
Vha464I CTTAAG 1 cut(s) 5084
VneI GTGCAC 1 cut(s) 3867
VpaK11BI GGWCC 2 cut(s) 1001, 2849
XagI CCTNNNNNAGG 1 cut(s) 1561
XapI RAATTY 7 cut(s) 898, 1543, 2646, 2838, 3303, 4252, 5303
XbaI TCTAGA 2 cut(s) 2884, 4270
XceI RCATGY 5 cut(s) 536, 2966, 3350, 5160, 5238
XcmI CCANNNNNNNNNTGG 1 cut(s) 4369
XhoI CTCGAG 1 cut(s) 4844
XmiI GTMKAC 2 cut(s) 1518, 1948
XmnI GAANNNNTTC 6 cut(s) 1031, 2979, 3975, 4200, 5131, 5468
ZrmI AGTACT 1 cut(s) 3243
Zsp2I ATGCAT 3 cut(s) 2690, 4226, 4308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.